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| 1 | Knockdown of ecdysis-triggering hormone gene with a binary UAS/GAL4 RNA interference system leads to lethal ecdysis deficiency in silkworm显示文摘被触发蜕皮的荷尔蒙(ETH ) 是在大多数昆虫的蜕皮过程的一个集成因素,包括 Bombyx 粗腐殖质 i (蚕) 。在蚕理解 ETH 基因的函数,我们开发了一条有效途径到击倒在 vivo 的 ETH 的表达式成功地在另外的昆虫种类基于 RNA 干扰(RNAi ) 和是的一个二进制 UAS/GAL4 表达式系统使用了。二种转基因的蚕与这个方法被建立:有在 UAS 的控制下面的 ETH RNAi 顺序的受动器紧张和有编码的 GAL4 的使活跃之物紧张在 Bombyx 粗腐殖质 i 的控制下面定序细胞质的 actin3。由穿过二紧张,双 positive 转基因的蚕被获得,并且他们的 ETH 表示被发现比每个单身的积极转基因的父母的戏剧性地低。证明致命到双 positive 的严重蜕皮缺乏在 pharate 秒中间形态幼虫的阶段的转基因的蚕,当单个积极的转基因或野类型的蚕有正常蜕皮时。这条 UAS/GAL4 RNAi 途径提供一个方法在不同发展阶段学习内长的蚕基因的功能。 | Hongjiu Dai Li Ma Jue Wang Rongjing Jiang Zhugang Wang Jian Fei | 2008 | Acta Biochimica et Biophysica Sinica2008，40，9： | 12 |
| 2 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 3 | In situ generated thrombin in the protein corona of zeolites: Relevance of the functional proteins to its biological impact显示文摘到 nanomaterial 表面的血浆蛋白质的吸附在他们的简历功能上有大影响。然而,有在在蛋白质日冕和材料的生物身份的功能的蛋白质之间的关系的有限理解。这里,我们证明在里面 situ 在 Ca 沸石表面的蛋白质日冕产生了凝血酵素显示一个钙依赖者,不平常地高(3,000 NIH U/mg ) procoagulant 活动,它对 antithrombin 释放是甚至稳定的。移开包含的 Ca < 啜 class= “ a-plus-plus ” > 在沸石的 2+ 由 antithrombin 导致释放。我们的观察建议凝血酵素活动能被无机的表面和阳离子调整。最重要地,我们的发现在蛋白质日冕和材料的 procoagulant 活动显示在 biomolecules 之间的连接,提供为沸石 hemostatics 的 procoagulant 机制的一个新分子的基础。 | Yunlong Li Xiaofeng Liao Xiaoxi Zhang Guicen Ma Shuai Zuo Liping Xiao Galen D. Stucky Zhugang Wang Xian Chen Xiaoqiang Shang Jie Fan | 2014 | Nano Research2014，7，10： | 5 |
| 4 | Enhanced learning and memory in GAT1 heterozygous mice显示文摘-Aminobutyric 酸(伽马氨基丁酸) 是在中央神经系统的主要禁止的 neurotransmitter。伽马氨基丁酸传播的结束通过膜蛋白质的一个家庭的行动,叫的伽马氨基丁酸 transporters (GAT14 ) 。伽马氨基丁酸系统涉及存储器的调整,这很好被建立。我们的以前的学习显示出老鼠展出了损害马头鱼尾的怪兽依赖者学习和记忆的那同型结合的 GAT1/ 。在鼠标上评估内长的减少的伽马氨基丁酸举起的影响认知行为,学习和异质接合的 GAT1+/ 鼠标的存储器的能力被被动回避范例和莫利斯舞水迷宫检测。洞板范例也被用来在如此的老鼠在焦虑相关的行为或探索行为测量变化。作为 synaptic 粘性的一种形式,长期的 potentiation 在老鼠被记录海马趾的 CA1 区域。我们发现了显示的老鼠增加了的那 GAT1+/ 学习并且记忆,减少的像焦虑的行为,和与野类型、同型结合的 GAT1/ 老鼠相比的最高的 synaptic 粘性。我们的结果建议在 GAT1 活动的中等减小引起在鼠标学习和记忆的改进。 | Jun Shi Youqing Cai Guoxiang Liu Neng Gong Zhenze Liu Tianle Xu Zhugang Wang Jian Fei | 2012 | Acta Biochimica et Biophysica Sinica2012，44，4： | 3 |
| 5 | Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice显示文摘Recombinant 熔化蛋白质广泛地被用作抗原对目标蛋白质的 epitope 提起抗体。然而,在产生抗血清的伴随物反搬运人抗体减少需要的抗体的生产并且引起讨厌的非特定的有免疫力的反应。这被建议搬运人蛋白质转基因的动物能被用来解决这个问题。验证这个假设,提高的绿荧光灯的蛋白质(EGFP ) 转基因的老鼠被生产。由与熔化蛋白质 His6HAtag-EGFP 使老鼠免疫,我们证明从转基因的老鼠的抗血清从野类型的老鼠与那相比对 EGFP 对 His6HA 标签和更低的 titer 抗体有更高的 titer 抗体。因此,这份报告描述一个改进方法用能在抗体准备有申请潜力的 EGFP 转基因的老鼠提起高 titer 反肽 polyclonal 抗体。 | Jianke Ren Long Wang Guoxiang Liu Wen Zhang Zhejin Sheng Zhugang Wang Jian Fei | 2008 | Acta Biochimica et Biophysica Sinica2008，40，2： | 3 |
| 6 | Efficient and stable genera-tion of transgenic silkworm, Bombyx mori with the lepi-dopteran derived transposon piggyBac显示文摘The piggyBac transposable element was suc-cessfully used for generation of the transgenic silkworm, Bombyx mori. The EGFP was adopted in this study as a screening marker in transgenic vector under the control of an artificial promoter containing Pax-6 binding sites that can drive eye-specific genes expression in various insect species including B. mori and Drosophila. 111 independent trans-genic lines were obtained among 700 fertile G0 moths. Most of the transgenic lines contained two or more chromosomal insertions of the EGFP marker, which were stably inherited over more than six generations during the time of this pro-ject. PiggyBac-mediated transposition was confirmed by identifying the characteristic TTAA duplication sequence at the insertion sites. The stable and high efficient transmission of a genetic marker into B. mori confirms the usage of this vector-marker system for industrial production and theo-retical research. | DAI Hongjiu XU Guojiang Thomas Jean-luc WANG Zhugang JIANG Rongjing FEI Jian | 2005 | Chinese Science Bulletin2005，50，15： | 2 |
| 7 | PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome显示文摘Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Ran- dom integration of transgene often results in inser- tional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However,the utilization of these potentially valuable resources is hampered due to lack of efficient ap- proaches for rapid identification of multi-copy trans- gene integration sites. Here we propose two PCR-based approaches to identifying trans- gene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them,the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient ap- proaches will greatly facilitate the study of insertional mutation due to transgene integration. | ZHAO Xudong DANG Suying LIANG Bin LEI Xia CHEN Zheng WANG Long YAN Lanzhen SUN Hantang FU Jiliang FEI Jian WANG Zhugang | 2006 | Chinese Science Bulletin2006，51，18： | 2 |
| 8 | A C-terminal truncated mutation of spr-3 gene extends lifespan in Caenorhabditis elegans显示文摘Caenorhabditis elegans 的 lifespan 被各种各样的基因、环境的因素决定。在这篇论文, spr-3, C。elegans 哺乳动物的神经限制 silencing 因素(NRSF/REST ) 的相应基因,被报导是调整 C 的 lifespan 的重要基因。elegans。spr-3, spr-3 (ok2525 ) ,或 spr-3 表示的 RNAi 抑制的一个删除变化在 C 导致了短 lifespan 显型。elegans。然而, spr-3 的一个胡说八道变化, spr-3 (by108 ) ,增加了 lifespan 26% 什么时候与野类型的线虫的相比。spr-3 (by108 ) 也显示出增加的抵抗到环境应力。spr-3 (by108 ) 变异基因编码 C 终端有与 REST4 可比较的结构的截断的蛋白质, NRSF/REST 在的 splice 变体哺乳动物。spr-3 (by108 ) 的长 lifespan 显型异种在 daf-16 和 glp-1 的正常功能上作为功能和依赖者的获得被证实。spr-3 (by108 ) 的 lifespan 能被在 daf-2 导致一个变化 synergistically 提高。量的聚合酶链反应结果证明 daf-16 以及它的目标基因 sod-3, mtl-1,和 sip-1 的表示在 spr-3 (by108 ) 是起来调整的变异。这些结果将是有用的进一步理解 NRSF/REST 基因的复杂功能在哺乳动物,特别在变老的过程和长寿决心。 | Ping Yang Ruilin Sun Minghui Yao Weidong Chen Zhugang Wang Jian Fei | 2013 | Acta Biochimica et Biophysica Sinica2013，45，7： | 2 |
| 9 | MicroRNA-449 and MicroRNA-34b/c Function Redundantly in Murine Testes by Targeting E2F Transcription Factor-Retinoblastoma Protein (E2F-pRb) Pathway显示文摘 | Jianqiang Bao Ding Li Li Wang Jingwen Wu Yanqin Hu Zhugang Wang Yan Chen Xinkai Cao Cizhong Jiang Wei Yan Chen Xu | 2012 | Journal of Biological Chemistry2012，，26： | 2 |
| 10 | NRSF/REST is required for gastrulation and neurogenesis during zebrafish development显示文摘抑压者元素 1 沉默抄写因素(休息) 作为调整神经系统区别的抄写 suppressor 被认出。然而,休息的角色没在早开发期间被澄清。我们克隆人的休息的 zebrafish 相当或相同的事物。实时聚合酶链反应结果证明 zebrafish 休息 mRNA 是母亲的并且有到迟了的分割时期的从胞胚的更高的表示水平的 zygotic。在杂交显示出的 situ 整个山自从屋顶舞台,那休息强烈在胚盘被表示并且动态地主要在开发大脑表示了,特别在在早分割时期的大脑分的边阶。用堵住 morpholino (MO-tra ) 的翻译的休息击倒技术导致了 gastrulation 延期或甚至阻塞,并且随后与缺乏的神经发生由早分割时期导致了胚胎致命性。然而,拼接为休息堵住 morpholino 没显示出显然反常的显型直到 48 hpf (小时授精以后) ,显示那母亲的休息是为 gastrulation 的一个重要管理者。进一步的学习表明在 MO-tra morphants 的反常发展是至少部分由于到胚盘的从蛋黄的蛋白质交通的机能障碍。我们的结果证明那休息(特别母亲的供应休息) 早在 zebrafish 期间为 gastrulation 和神经发生被要求 embryogenesis。 | Xuesong Wang Jianke Ren Zhugang Wang Jihua Yao Jian Fei | 2012 | Acta Biochimica et Biophysica Sinica2012，44，5： | 1 |
| 11 | STAT3 and AKT signaling pathways mediate oncogenic role of NRSF in hepatocellular carcinoma显示文摘Neuron-restrictive silencer factor(NRSF)is a zinc finger protein that acts as a negative transcriptional regulator by recruiting histone deacetylases and other co-factors.It plays a crucial role in nervous system development and is recently reported to be involved in tumorigenesis in a tumor type-dependent manner;however,the role of NRSF in hepatocellular carcinoma(HCC)tumorigenesis remains unclear.Here,we found that NRSF expression was up-regulated in 27 of 49 human HCC tissue samples examined.Additionally,mice with conditional NRSF-knockout in the liver exhibited a higher tolerance against diethylnitrosamine(DEN)-induced acute liver injury and were less sensitive to DEN-induced HCC initiation.Our results showed that silencing NRSF in HepG2 cells using RNAi technology significantly inhibited HepG2 cell proliferation and severely hindered their migration and invasion potentials.Our results demonstrated that NRSF plays a pivotal role in promoting DEN-induced HCC initiation via a mechanism related to the STAT3 and AKT signaling pathways.Thus,NRSF could be a potential therapeutic target for treating human HCC. | Ming Ma Yunhe Zhou Ruilin Sun Jiahao Shi Yutong Tan Hua Yang Mengjie Zhang Ruling Shen Leon Xu Zhugang Wang Jian Fei | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 1 |
| 12 | Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spotl4 and Cyp2e1 expression levels显示文摘Mitochondrion-localized retinol dehydrogenase 13 (Rdh13) is a short-chain dehydrogenase/reductase involved in vitamin A metabolism in both humans and mice. We previously generated Rdh13 knockout mice and showed that Rdh13 deficiency causes severe acute retinal light damage. In this study, considering that Rdh13 is highly expressed in mouse liver, we further evaluated the potential effect of Rdh13 on liver injury induced by carbon tetrachloride (CC14). Although Rdh13 deficiency showed no significant effect on liver histology and physiological functions under regular culture, the Rdh13^-/- mice displayed an attenuated response to CCl4-induced liver injury. Their livers also exhibited less histological changes and contained lower levels of liver-related metabolism enzymes compared with the livers of wild-type (WT) mice. Furthermore, the Rdhl3 1 mice had Rdh13 deficiency and thus their liver cells were protected from apoptosis, and the quantity of their proliferative cells became lower than that in WT after CC14 exposure. The ablation of Rdhl3 gene decreased the expression levels of thyroid hormone-inducible nuclear protein 14 (Spot14) and cytochrome P450 (Cyp2el) in the liver, especially after CC14 treatment for 48 h. These data suggested that the alleviated liver damage induced by CC14 in Rdh13^-/- mice was caused by Cyp2el enzymes, which promoted reductive CC14 metabolism by altering the status of thyroxine metabolism. This result further implicated Rdhl3 as a potential drug target in preventing chemically induced liver injury. | Xiaofang Cui Benting Ma Yan Wang Yan Chen Chunling Shen Ying Kuang Jian Fei Lungen Lu Zhugang Wang | 2019 | Frontiers of Medicine2019，13，1： | 1 |
| 13 | A Blind Frequency Offset Estimator for Coherent M-PSK System in Autonomous Radio显示文摘 | Le Wang Zhugang Wang Weiming Xiong | 2013 | Circuits Systems and Signal Processing2013，，3： | 1 |
| 14 | Knockout of Abi3bp in mice does not affect their olfactory function, mental state and NNK-induced lung tumorigenesis显示文摘Abi3bp 原来作为 Abi3-Src 相同 3 被发现(SH3 ) 有约束力的蛋白质并且被证明了在成年纸巾有宽广表示侧面。尽管以前的研究显示了 Abi3bp 可以与癌症抑制,房间老朽,树枝状的精炼和心理混乱被联系,完成的大多数结论在 vitro 基于模型或染色体宽的协会学习。在这个工作,我们构造了一个 Abi3bp 缺乏的老鼠模型并且观察了 phenotypic 变化。产生 Abi3bp 大美人鼠标可行、肥沃,通常发展并且不展出焦虑或像消沉的行为地的重要差别,嗅觉的函数和肿瘤发生。这些数据建议 Abi3bp 在的功能在模型不在未经触动的动物翻译到一个类似的角色的 vitro。它的弄空可以被另外的基因补偿,它需要在未来研究被探讨。 | Hua Yang Weidong Chen Jiahao Shi Dandan Huang Jun Li Binyang Hu Mengjie Zhang Zhugang Wang Jian Fei | 2016 | Acta Biochimica et Biophysica Sinica2016，48，9： | 0 |
| 15 | Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes显示文摘Monocyte chemoattractant protein-1(MCP-1)plays a crucial role in various inflammatory diseases.To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents,we establish a transgenic mouse line.The firefly luciferase gene is incorporated into the mouse genome and driven by the endogenous MCP-1 promoter.A bioluminescence photographing system is applied to monitor luciferase levels in live mice during inflammation,including lipopolysaccharide-induced sepsis,concanavalin A-induced T cell-dependent liver injury,CCl_(4)-induced acute hepatitis,and liver fibrosis.The results demonstrate that the luciferase signal induced in inflammatory processes is correlated with endogenous MCP-1 expression in mice.Furthermore,the expressions of MCP-1 and the luciferase gene are dramatically inhibited by administration of the anti-inflammatory drug dexamethasone in a septicemia model.Our results suggest that the transgenic MCP-1-Luc mouse is a useful model to study MCP-1 expression in inflammation and disease and to evaluate the efficiency of anti-inflammatory drugs in vivo. | Fangyang Shao Lei Ci Jiahao Shi Fei Fang Bowen Yan Xijun Liu Xiangyu Yao Mengjie Zhang Hua Yang Zhugang Wang Jian Fei | 2022 | Acta Biochimica et Biophysica Sinica2022，54，10： | 0 |
| 16 | Establishment, characterization, and application of pAcr-SP-NTP-EGFP transgenic mice in visualizing the oviduct-migrating ability of sperm from Prss37-null mice显示文摘 | Chunling Shen Jinjin Wang Hua Zhuang Jianbing Liu Xiyi Wang Xuejiao Chen Zhuanbin Wu Wenting Wu Youbing Wu Yanwen Sun Huimin Yan Jian Fei Ying Kuang Zhugang Wang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，6： | 0 |
| 17 | Gpr97/Adgrg3 ameliorates experimental autoimmune encephalomyelitis by regulating cytokine expression显示文摘 | Jinjin Wang Xiyi Wang Xuejiao Chen Shunyuan Lu Ying Kuang Jian Fei Zhugang Wang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，7： | 0 |
| 18 | Tafa-2 plays an essential role in neuronal survival and neurobiological function in mice显示文摘 | Xiyi Wang Chunling Shen Xuejiao Chen Jinjin Wang Xiaofang Cui Yicheng Wang Hongxin Zhang Lingyun Tang Shunyuan Lu Jian Fei Zhugang Wang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，10： | 0 |
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