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| 1 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 2 | METHOD FOR PRECISE CALCULATION OF ROOT STRESS OF SPIRAL BEVEL GEARS显示文摘METHODFORPRECISECALCULATIONOFROOTSTRESSOFSPIRALBEVELGEARSMETHODFORPRECISECALCULATIONOFROOTSTRESSOFSPIRALBEVELGEARSChenLiangyu... | Chen Liangyu Wang Yanzhong Zheng Xijian E Zhongkai Cai Chunyuan(Northeastern University) | 1994 | Chinese Journal of Mechanical Engineering1994，7，4： | 5 |
| 3 | Effect of controlled low central venous pressure on renal function in major liver resection显示文摘Objective: To investigate the effects of low central venous pressure (LVCP) on blood loss and evaluate its influ- ence on renal function in patients undergoing hepatectomy. Methods: Forty-six patients, ASA classification I–III, undergoing liver resection were randomized into LCVP group (n = 23) and control group (n = 23). In LCVP group, CVP was maintained at 2–4 mmHg and MBP above 60 mmHg during hepatectomy, while in control group hepatectomy was performed routinely without lowering CVP. Volume of blood loss during hepatectomy, volume of blood transfusion, and changes of renal functions were compared between the two groups. Results: There were no significant differences in demographics, ASA score, type of hepatectomy, duration of inflow occlusion, operation time, weight of resected liver tissues, and renal functions between the two groups. LCVP group had a significantly lower volume of total intraoperative blood loss (P < 0.01) and RBC transfusion (P < 0.05). Conclusion: Lowering the CVP to less than 5 mmHg is a simple and effective technique to reduce blood loss and blood infusion during liver resection, and has no detrimental effects on renal functions. | Yuyong Liu Mingxue Cai Shan'e Duan Xuemei Peng Yong Lai Yalan Li | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，1： | 5 |
| 4 | Chaperone-mediated autophagy:roles in neuroprotection显示文摘Chaperone-mediated autophagy(CMA), one of the main pathways of lysosomal proteolysis, is characterized by the selective targeting and direct translocation into the lysosomal lumen of substrate proteins containing a targeting motif biochemically related to the pentapeptide KFERQ. Along with the other two lysosomal pathways, macro- and micro-autophagy, CMA is essential for maintaining cellular homeostasis and survival by selectively degrading misfolded, oxidized, or damaged cytosolic proteins. CMA plays an important role in pathologies such as cancer, kidney disorders, and neurodegenerative diseases. Neurons are post-mitotic and highly susceptible to dysfunction of cellular quality-control systems. Maintaining a balance between protein synthesis and degradation is critical for neuronal functions and homeostasis. Recent studies have revealed several new mechanisms by which CMA protects neurons through regulating factors critical for their viability and homeostasis. In the current review, we summarize recent advances in the understanding of the regulation and physiology of CMA with a specific focus on its possible roles in neuroprotection. | Zhibiao Cai Weijun Zeng Kai Tao Zhen E Bao Wang Qian Yang | 2015 | Neuroscience Bulletin2015，31，4： | 4 |
| 5 | CCAR1 5' UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance显示文摘 | Gaopeng Li Xiaoli Wu Wenchang Qian Huayong Cai Xinbao Sun Weijie Zhang Sheng Tan Zhengsheng Wu Pengxu Qian Keshuo Ding Xuefei Lu Xiao Zhang Hong Yan Haifeng Song Shouhong Guang Qingfa Wu Peter E Lobie Ge Shan Tao Zhu | 2016 | Cell Research2016，26，6： | 3 |
| 6 | Efficacy and Safety of Yanggan Jian in Hepatitis B Virusrelated Decompensated Cirrhosis:A Randomized,Doubleblind,Controlled Trial显示文摘Background and Aims:The aim was to evaluate the efficacy and safety of Yanggan Jian(YGJ)in HBV-infected patients with decompensated cirrhosis.Methods:This randomized,double-blind controlled trial enrolled 160 patients with HBV-related decompensated cirrhosis who were already receiving or about to start antiviral therapy.Patients were randomly assigned to receive YGJ or placebo for 24 weeks,and were followed-up to 36 weeks.The primary outcome was the proportion of patients with a≥2 point reduction in Child-Turcotte-Pugh(CTP)score from baseline at week 24.Secondary outcomes were CTP class and score,serum liver function indices,mortality,incidence of hepatocellular carcinoma and variceal bleeding.Results:The proportion of patients with a CTP score reduction≥2 was significantly greater in the YGJ than in the placebo group(p=0.009);the percentage of patients with CTP class C was significantly less than that in the placebo group(p<0.05),and the YGJ group had a significantly greater mean change from baseline in CTP score at week 24(p=0.034).The improvement in measured values and change from baseline of prothrombin time,serum albumin,platelets,cholinesterase,international normalized ratio,and activated partial thromboplastin time were significantly better with YGJ than with placebo.Between-group differences in cumulative rates of variceal bleeding,hepatocellular carcinoma,death,or the frequency of any adverse event(AE),AEs related to treatment,or discontinuation because of AEs were not significant.Conclusions:YGJ significantly improved CTP scores and hepatic synthetic and reserve function in patients with HBV-related decompensated cirrhosis,and was safe and well tolerated. | Long Chen Chaoqun Zhao Weili Yao Wei Liu Hua Zhang Yongping Mu Hong Cai Dongying Xue Chengbao Wang Wan’e Wang Yuehong Lin Jiamei Chen Ping Liu | 2023 | Journal of Clinical and Translational Hepatology2023，11，1： | 3 |
| 7 | DNA electrochemical biosensors for environmental monitoring 显示文摘 | Wang J Rivas Cai G Palecek E Nielsem | 1997 | A Review Analytical Chemica Acta1997，347，12： | 1 |
| 8 | Minor H antigen HA-l-specific regulator and effector CD8+ T cells,and HA-1microchimerism,in allograft tolerance显示文摘 | Cai J Lee J Jankowska-Gan E | 2004 | J Exp Med2004，199，7： | 1 |
| 9 | A fem that hyperaccumulates arsenic显示文摘 | Ma L Q Komar K M Tu C Zhang W H Cai Y Kennelley E D | 2001 | Nature2001，409，： | 1 |
| 10 | New isomers of poly(vinyl fluoride) with controlled regiosequence microstructure显示文摘 | Cais R E Kometani J M | 1988 | Polymer1988，29，1： | 1 |
| 11 | Modern tidal rhythmites deposited in a deep-water estuaty显示文摘 | Cowan E A Cai J Powell R D | 1998 | Geo-marine Letters1998，18，： | 1 |
| 12 | Cancer chemopreventive activity of resveratrol, a natural product derived from grapes 显示文摘 | JANG M S CAI E N UDEANI G O | 1997 | Science1997，275，： | 1 |
| 13 | Food as a form of destination identity: A tourism destination brand perspective 显示文摘 | Lin Y C Pearson T E Cai L P | 2011 | Tourism and Hospitality Research2011，1，1： | 1 |
| 14 | Studies on the Automated Determination of Total Rare Earths in Al-Re Alloys by Automated Flow Injection Device with DBC-CPA Reagent显示文摘 | Chen Danhua Cai Ruxiu and Zeng Yun’e(Department of Chemistry Wuhan University Wuchang) | 1988 | Chemical Journal of Chinese Universities1988，，02： | 1 |
| 15 | Large-area synthesis of high-quality and uniform graphene films on copper foils显示文摘 | LI Xue-song CAI Wei-wei AN Jin-ho KIM S NAH J YANG Dong-xing P1NER R VELAMAKANNI A JUNG I TUTUC E BANERJEE S K COLOMBO L RUOFF R S | 2009 | Science2009，324，5932： | 1 |
| 16 | A singular value thresh-olding algorithm for matrix completion 显示文摘 | CAI J CANDES E SHEN Z | 2010 | SIAM Journal on Optimization2010，20，4： | 1 |
| 17 | The Glu298Asp (894G/T)mutation at exon 7 of the endothelial nitric oxide synthase gene and coronary artery disease显示文摘 | Cai H Wilcken D E Wang X L | 1999 | J Mol Med1999，77，6： | 1 |
| 18 | PET imaging and optical imaging with D-lueiferin methyl es- ter and D-luciferin methyl ether of luciferase gene expression in tumor xenografts of living mice 显示文摘 | Wang J-Q Pollok K E Cai S | 2006 | Bioorganic & Medicinal Chemistry Letters2006，16，2： | 1 |
| 19 | Optimization of poly- saccharides extraction from Gynostemma pentaphyllum Makino using uniform design 显示文摘 | Wang ZJ Luo DH Cai E'N | 2007 | Carbohydrate Polymers2007，69，2： | 1 |
| 20 | Charged nanoparticles as protein delivery systems: a feasibility study using lysozyme as model protein 显示文摘 | Cai CF Bakowsky U Rytting E | 2008 | Eur J Pharm Biopharm2008，69，： | 1 |
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