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| 1 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 2 | Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors显示文摘Multipotent 神经干细胞为房间治疗保持大诺言。到导致的 pluripotent 干细胞以及成熟神经原的成纤维细胞的 reprogramming 建议可能性没有首先建立 pluripotency,把一个严重地区分的体的房间变换成一个 multipotent 状态。这里,我们证明房间从中层导出的那 Sertoli 能直接被变换成拥有神经干细胞性质的一个 multipotent 状态。导致的神经干细胞(iNSCs ) 表示多重 NSC 特定的标记,展出类似于正常 NSC 的全球基因表示侧面,并且能够自强并且区分功能的神经原进神经胶质并且 electrophysiologically。为酷氨酸 hydroxylase (TH ) 积极的导出 iNSC 的神经原污点, γ -aminobutyric 酸,和胆碱 acetyltransferase。另外, iNSCs 能熬过并且产生跟随移植进有牙齿的回转的触处。iNSCs 的产生可以为疾病建模和再生药有重要含意。 | Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Libin Wang Wei Li Haijiang Zhang Xiao-Yang Zhao Lei Liu Ziwei Wang Changlong Guo Hua-Jun Wu Zhonghua Liu Liu Wang Shigang He Xiu-Jie Wang Zhiguo Chen Qi Zhou | 2012 | Cell Research2012，22，1： | 31 |
| 3 | Genetic correction of p-thalassemia patient-specific iPS cells and its use in improving hemoglobin production in irradiated SCID mice显示文摘 | Yixuan Wang Chen-Guang Zheng Yonghua Jiang Jiqin Zhang Jiayu Chen Chao Yao Qingguo Zhao Sheng Liu Ke Chen Juan Du Ze Yang Shaorong Gao | 2012 | Cell Research2012，22，4： | 27 |
| 4 | Construction, expression and characterization of the engineered antibody against tumor surface antigen, p185^(c-erbB-2)显示文摘The c-erbB-2 proto-oncogene encodes a 185kDa protein p185, which belongs to epidermal growth factorreceptor family. Amplification of this gene has been shown to correlate with poor clinical prognosis forcertain cancer patients. The monoclonal antibody A21 which directed against p185 specifically inhibitsproliferation of tumor cells overexpressing p185, hence allows it to be a candidate for targeted therapy. Inorder to overcome several drawbacks of murine MAb, we cloned its VH and VL genes and constructed thesingle-chain Fv (scFv) through a peptide linker. The recombinant scFvA21 was expressed in Escherichiacoli and purified by the affinity column. Subsequently it was characterized by ELISA, Western blot, cellimmunohistochemistry and FACS. All these assays showed the binding activity to extracellular domain(ECD) of p185. Based on those properties of scFvA21, we further constructed the scFv-Fc fusion moleculewith a homodimer form and the recombinant product was expressed in mammalian cells. In a series ofsubsequent analysis this fusion protein showed identical antigen binding site and activity with the parentantibody. These anti-p185 engineered antibodies have promised to be further modified as a tumor targetingdrugs, with a view of application in the diagnosis and treatment of human breast cancer. | LIAN SHENG CHENG, AI PING LIU, JIA HONG YANG, YAN QIU DONG, LIANG WEI LI, JING WANG, CHAO CHEN WANG, JING LIUSchool of Life Science, University of Science and Technology of China, Hefei 230027, China | 2003 | Cell Research2003，13，1： | 24 |
| 5 | Impact of social isolation and resident intruder stress on aggressive behavior in the male rat显示文摘Stress studies frequently utilize physical stressors to establish animal models of stress.In the majority of cases,these models are not consistent with human circumstances.The present study simulated a social isolation plus resident intruder stress model in the rat.The rats were subjected to daily social isolation and resident intruder stress for 2 weeks.Behaviors were then tested.Rats subjected to social stress exhibited different aggressive behavior styles;some rats had greater scores on composite aggression behaviors,as well as locomotor and exploratory activity,but lower scores on latency than others.The high-aggressive group exhibited predominantly anger-out,while the low-aggressive group exhibited anger-in.Results suggest that social isolation plus resident intruder may serve as an appropriate model for anger-in and anger-out emotion modeling in the rat. | Sheng Wei Huiyun Zhang Jie Gao Ling Xue Peng Sun Yubin Chao Gang Xue Mingqi Qiao | 2010 | Neural Regeneration Research2010，5，15： | 23 |
| 6 | Overexpression of P53 and its risk factors in esophageal cancer in urban areas of Xi′an显示文摘OverexpressionofP53anditsriskfactorsinesophagealcancerinurbanareasofXi′anQIAOGuiBin1,HANChengLong2,JIANGRenChao1,SUNChang?.. | QIAO GuiBin1, HAN ChengLong2, JIANG RenChao1, SUN ChangSheng3, WANG Yan3 and WANG YunJie3 | 1998 | World Journal of Gastroenterology1998，4，1： | 21 |
| 7 | Generation of dopaminergic neurons directly from mouse fibroblasts and fibroblast-derived neural progenitors显示文摘 | Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Lisi Sang Libin Wang Changlong Guo Wanwan Zhu Man Tong Lei Liu Wei Li Zhong-Hua Liu Xiao-Yang Zhao Liu Wang Zhiguo Chen Qi Zhou | 2012 | Cell Research2012，22，4： | 12 |
| 8 | Microporous bamboo biochar for lithium-sulfur batteries显示文摘简单、便宜、可伸缩、环境地友好,多微孔的生物资源 biochars 在锂硫(Li-S ) 为申请一直在吸引热心注意电池。此处,多孔的竹子 biochar 经由创造多微孔的结构的一个 KOH/annealing 过程被激活,增加表面区域并且提高电子电导率。对待的样品被用来包含硫准备多微孔的竹子碳硫(英国计算机学会) 用作为 Li-S 电池的阴极的 nanocomposite 第一次。有 50 wt.% 硫内容的英国计算机学会 nanocomposite 交付 1,295 妈的一个高起始的能力 ??? ?? | Xingxing Gu Yazhou Wang Chao Lai Jingxia Qiu Sheng Li Yanglong Hou Wayde Martens Nasir Mahmood Shanqing Zhang | 2015 | Nano Research2015，8，1： | 11 |
| 9 | A Facile Synthesis of Monodisperse Au Nanoparticles and Their Catalysis of CO Oxidation显示文摘Monodisperse Au nanoparticles(NPs)have been synthesized at room temperature via a burst nucleation of Au upon injection of the reducing agent t-butylamine-borane complex into a 1,2,3,4-tetrahydronaphthalene solution of HAuCl4·3H2O in the presence of oleylamine.The as-synthesized Au NPs show size-dependent surface plasmonic properties between 520 and 530 nm.They adopt an icosahedral shape and are polycrystalline with multiple-twinned structures.When deposited on a graphitized porous carbon support,the NPs are highly active for CO oxidation,showing 100%CO conversion at-45°C. | Sheng Peng Youngmin Lee Chao Wang Hongfeng Yin Sheng Dai Shouheng Sun | 2008 | Nano Research2008，1，3： | 11 |
| 10 | Analysis of a Propeller Wake Flow Field Using Viscous Fluid Mechanics显示文摘计算液体动力学(CFD ) 方法被用来数字地模仿一个推进器在开的水里弄醒流动地。一个子域混血儿网孔方法在这篇论文被采用。计算领域被分开成二子域,有四面的元素在内部领域在被使用匹配推进器的复杂几何学,当具有六面的元素在外部领域被使用时。网孔局部地在推进器表面上并且在 wake 流动域附近被精制,并且一个尺寸函数被用来控制格子的生长率。在不同轴的地点的节被用来学习推进器的空间进化在下游地从圆盘到一推进器直径(D) 的区域醒来。数字结果证明轴的速度沿着 wake 流动波动;光线的速度,它是仔细与旋涡有关,强烈稀释。后面的旋涡在落后于边的片与尖端旋涡交往然后分开。旋涡的力量很快缩小,并且半径减少 20% 在一条直径下游。 | Miao He Chao Wang Xin Chang Sheng Huang | 2012 | Journal of Marine Science and Application2012，11，3： | 10 |
| 11 | Rhynchophylla total alkaloid rescues autophagy, decreases oxidative stress and improves endothelial vasodilation in spontaneous hypertensive rats显示文摘Autophagy 在减轻氧化应力并且稳定动脉粥样硬化患者匾起一个重要作用。然而,在 endothelial vasodilation 功能的 autophagy 的潜在的角色很少被学习了。这研究试图调查 rhynchophylla 总数碱(RTA ) 是否在通过减少的氧化应力提高 autophagy,并且改进 endothelial vasodilation 有一个积极角色。在氧化低密度的脂蛋白(ox-LDL ) 对待人的脐的静脉 endothelial 房间(HUVEC ) , RTA (200 mg/L ) 显著地压制了 ox-LDL-induced 通过 rescuing autophagy 的氧化应力,和减少的房间 apoptosis。在自发的高血压的老鼠(SHR ) , RTA 的管理(50 mgy 设备。测试配置包括变化倾斜角度,热有效性控制,改变的管方面比率和改变的空气卷流动率和对国内规模除去湿气系统适用的潮湿的空气条件。研究调查结果表演一谦虚用 P 倾斜多达 20 的角度。感染 falciparum 的正常红血球,我们证明 API 的增加的集中禁止了增长 chloroquine 易受影响(3D7 ) 并且抵抗(Dd2 ) 在文化与的寄生虫类似 50% 禁止的集中(IC 50) 价值(36.02 到赼?赼?? | Chao LI Feng JIANG Yun-lun LI Yue-hua JIANG Wen-qing YANG Jie SHENG Wen-juan XU Qing-jun ZHU | 2018 | Acta Pharmacologica Sinica2018，39，3： | 10 |
| 12 | Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation显示文摘Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding. | CHAO Shufen CAI Yicong FENG Baobing JIAO Guiai SHENG Zhonghua LUO Ju TANG Shaoqing WANG Jianlong HU Peisong WEI Xiangjin | 2019 | Rice science2019，26，2： | 10 |
| 13 | Smart City Architecture: A Technology Guide for Implementation and Design Challenges显示文摘Urban development is becoming increasingly reliant on effective use of intelligent services.In the process of providing better services to all citizens and improving the efficiency of administration processes,the concept of a smart city has been lauded as a promising solution for the coming challenge of global urbanization.However,due to the broad scope of smart cities their definition has not been standardized,thus proposed architectures are diverse.This work proposes a new architecture from the perspective of the data that underpins all the functionality of smart cities.The proposed architecture is discussed,outlining design challenges and offering insight into the development of smart cities. | RONG Wenge XIONG Zhang COOPER Dave LI Chao SHENG Hao | 2014 | China Communications2014，11，3： | 9 |
| 14 | Comparison of Xpert MTB/RIF, Real Amp, and CPA Tests in Detecting Mycobacterium tuberculosis显示文摘Today, tuberculosis (TB) remains a global public health threat associated with significantly high rates of morbidity and mortality. The World Health Organization's (WHO) Global Tuberculosis Report 2018[1] has reported that in 2017, 10.0 million people across the world had developed TB diseases that resulted in an estimated 1.6 million deaths, and 889, 000 people developed TB in China that led to 39, 000 TB-related deaths. Therefore, rapid and accurate detection of Mycobacterium tuberculosis (MTB) is important for initiating early treatment and reducing mortality. Traditional diagnostic methods for pulmonary TB incorporate chest radiography and sputum smear microscopy;however, several cases of tuberculosis go undiagnosed because of the low sensitivity of smear microscopy[2]. | OU Xi Chao LI Hui LIU Dong Xin XIA Hui MA Xiao Guang WANG Shao Hua WANG Sheng Fen ZHU Yan Kun ZHAO Bing ZHAO Yan Lin | 2019 | Biomedical and Environmental Sciences2019，32，3： | 9 |
| 15 | Deep Learning Based Attack Detection for Cyber-Physical System Cybersecurity:A Survey显示文摘With the booming of cyber attacks and cyber criminals against cyber-physical systems(CPSs),detecting these attacks remains challenging.It might be the worst of times,but it might be the best of times because of opportunities brought by machine learning(ML),in particular deep learning(DL).In general,DL delivers superior performance to ML because of its layered setting and its effective algorithm for extract useful information from training data.DL models are adopted quickly to cyber attacks against CPS systems.In this survey,a holistic view of recently proposed DL solutions is provided to cyber attack detection in the CPS context.A six-step DL driven methodology is provided to summarize and analyze the surveyed literature for applying DL methods to detect cyber attacks against CPS systems.The methodology includes CPS scenario analysis,cyber attack identification,ML problem formulation,DL model customization,data acquisition for training,and performance evaluation.The reviewed works indicate great potential to detect cyber attacks against CPS through DL modules.Moreover,excellent performance is achieved partly because of several highquality datasets that are readily available for public use.Furthermore,challenges,opportunities,and research trends are pointed out for future research. | Jun Zhang Lei Pan Qing-Long Han Chao Chen Sheng Wen Yang Xiang | 2022 | IEEE/CAA Journal of Automatica Sinica2022，9，3： | 9 |
| 16 | 6-Benzylaminopurine treatment maintains the quality of Chinese chive(Allium tuberosum Rottler ex Spreng.) by enhancing antioxidant enzyme activity显示文摘Chinese chive usually develops an off-flavor after a short storage time. To explore effective ways to maintain the postharvest quality of Chinese chive, the effect of exogenous application of 6-benzylaminopurine(6-BA) on postharvest quality and antioxidant activity of chive was evaluated, and the mechanism of the physiological responses of chive to 6-BA treatment was explored. Chives were sprayed for 10 min with 100, 300, or 500 mg L–1 6-BA or with alkaline solution as the control, then stored at(2±1)°C with a relative humidity(RH) of 80–85%. We found that 300 mg L–1 6-BA significantly delayed yellowing and chlorophyll degradation, maintained the total phenolic and flavonoid content, and improved the activities of antioxidant enzymes, including superoxide dismutase(SOD), catalase(CAT) and peroxidase(POD). In conclusion, we identified exogenous application of 6-BA as an effective method for maintaining postharvest quality of Chinese chive. In addition, our finding that the activities of antioxidant enzymes increase in response to exogenous 6-BA provides new insights into the mechanism of cytokinin-based postharvest fresh-keeping. | JIA Li-e LIU Sheng DUAN Xiao-ming ZHANG Chao WU Zhan-hui LIU Ming-chi GUO Shao-gui ZUO Jin-hua WANG Li-bin | 2017 | Journal of Integrative Agriculture2017，16，9： | 8 |
| 17 | Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet显示文摘Objective To investigate activation of brown adipose tissue(BAT) stimulated by medium-chain triglyceride(MCT). Methods 30 Male C57BL/6J obese mice induced by fed high fat diet(HFD) were divided into 2 groups, and fed another HFD with 2% MCT or long-chain triglyceride(LCT) respectively for 12 weeks. Body weight, blood biochemical variables, interscapular brown fat tissue(IBAT) mass, expressions of m RNA and protein of beta 3-adrenergic receptors(β3-AR), uncoupling protein-1(UCP1), hormone sensitive lipase(HSL), protein kinase A(PKA), and adipose triglyceride lipase(ATGL) in IBAT were measured. Results Significant decrease in body weight and body fat mass was observed in MCT group as compared with LCT group(P<0.05) after 12 weeks. Greater increases in IBAT mass was observed in MCT group than in LCT group(P<0.05). Blood TG, TC, LDL-C in MCT group were decreased significantly, meanwhile blood HDL-C, ratio of HDL-C/LDL-C and norepinephrine were increased markedly. Expressions of m RNA and protein of β3-AR, UCP1, PKA, HSL, ATGL in BAT were greater in MCT group than in LCT group(P<0.05). Conclusion Our results suggest that MCT stimulated the activation of BAT, possible via norepinephrine pathway, which might partially contribute to reduction of the body fat mass in obese mice fed high fat diet. | ZHANG Yong XU Qing LIU Ying Hua ZHANG Xin Sheng WANG Jin YU Xiao Ming ZHANG Rong Xin XUE Chao YANG Xue Yan XUE Chang Yong | 2015 | Biomedical and Environmental Sciences2015，28，2： | 8 |
| 18 | Behavioral Abnormality along with NMDAR-related CREB Suppression in Rat Hippocampus after Shortwave Exposure显示文摘Objective To estimate the detrimental effects of shortwave exposure on rat hippocampal structure and function and explore the underlying mechanisms. Methods One hundred Wistar rats were randomly divided into four groups(25 rats per group) and exposed to 27 MHz continuous shortwave at a power density of 5, 10, or 30 m W/cm^2 for 6 min once only or underwent sham exposure for the control. The spatial learning and memory, electroencephalogram(EEG), hippocampal structure and Nissl bodies were analysed. Furthermore, the expressions of N-methyl-D-aspartate receptor(NMDAR) subunits(NR1, NR2 A, and NR2 B), c AMP responsive element-binding protein(CREB) and phosphorylated CREB(p-CREB) in hippocampal tissue were analysed on 1, 7, and 14 days after exposure. Results The rats in the 10 and 30 m W/cm^2 groups had poor learning and memory, disrupted EEG oscillations, and injured hippocampal structures, including hippocampal neurons degeneration, mitochondria cavitation and blood capillaries swelling. The Nissl body content was also reduced in the exposure groups. Moreover, the hippocampal tissue in the 30 m W/cm^2 group had increased expressions of NR2 A and NR2 B and decreased levels of CREB and p-CREB. Conclusion Shortwave exposure(27 MHz, with an average power density of 10 and 30 m W/cm^2) impaired rats' spatial learning and memory and caused a series of dose-dependent pathophysiological changes. Moreover, NMDAR-related CREB pathway suppression might be involved in shortwave-induced structural and functional impairments in the rat hippocampus. | YU Chao BAI Yan Xin XU Xin Ping GAO Ya Bing HAO Yan Hui WANG Hui TAN Sheng Zhi LI Wen Chao ZHANG Jing YAO Bin Wei DONG Ji ZHAO Li PENG Rui Yun | 2019 | Biomedical and Environmental Sciences2019，32，3： | 7 |
| 19 | Simultaneous broadband generation of second and third harmonics from chirped nonlinear photonic crystals显示文摘Ultrabroadband laser sources are highly desirable in a wide variety of modern science disciplines ranging from physics,chemistry and materials science to information communications and processing.Here we present the design and fabrication of a chirped periodically poled lithium niobate(CPPLN)nonlinear photonic crystal that supports multiple orders of quasiphase matching with finite bandwidth and allows for the simultaneous broadband generation of second and third harmonics with high conversion efficiency.Moreover,the chirp rate has a significant influence on the conversion efficiency and bandwidth.The CPPLN scheme offers a promising approach for the construction of short-wavelength laser sources and enables the generation of the three primary colors—red,green and blue—from a single crystal,which may have potential applications in large-screen laser displays. | Bao-Qin Chen Ming-Liang Ren Rong-Juan Liu Chao Zhang Yan Sheng Bo-Qin Ma Zhi-Yuan Li | 2014 | Light(Science & Applications)2014，3，1： | 7 |
| 20 | Systematic review of laparoscopy-assisted versus open gastrectomy for advanced gastric cancer显示文摘Objective:The study compared laparoscopy-assisted gastrectomy(LAG) with open gastrectomy(OG) in the management of advanced gastric cancer(AGC).Methods:Literature search was performed in the Medline,Embase,and Cochrane Library databases to identify control studies that compared LAG and OG for AGC.A meta-analysis was conducted to examine the surgical safety and oncologic adequacy,using the random-effect model.Results:Seven eligible studies including 815 patients were analyzed.LAG was associated with less blood loss,less use of analgesics,shorter time of flatus and periods of hospital stay,but longer time of operation.The incidence of most complications was similar between the two groups.However,LAG was associated with a lower rate of pulmonary infection(odds ratio(OR) 0.19;95% confidence interval(CI) 0.05 to 0.68;P<0.05).No significant differences were noted in terms of the number of harvested lymph nodes(weighted mean difference(WMD) 1.165;95% CI-2.000 to 4.311;P>0.05),overall mortality(OR 0.65;95% CI 0.39 to 1.10;P>0.05),cancer-related mortality(OR 0.64;95% CI 0.32 to 1.25;P>0.05),or recurrence(OR 0.62;95% CI 0.33 to 1.16;P>0.05).Conclusions:LAG could be performed safely for AGC with adequate lymphadenectomy and has several short-term advantages compared with conventional OG.No differences were found in long-term outcomes.However,these results should be validated in large randomized controlled studies(RCTs) with sufficient follow-up. | Long-yun YE Da-ren LIU Chao LI Xiao-wen LI Ling-na HUANG Sheng YE Yi-xiong ZHENG Li CHEN | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，6： | 6 |
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