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| 1 | Study of Cracking Mechanism and Wear Resistance in Laser Cladding Coating of Ni-based Alloy显示文摘Nickel-based alloy coatings were widely used for the remanufacturing of dies and moulds by laser cladding,but the crack sensitivity would be increase due to the higher strength and hardness,which reduced the wear resistance of Ni-based alloys.In this paper,Ni-based coatings with the addition of a plastic phase(an austenitic stainless net)were prepared using laser cladding technology,and the CeO_(2)was added in cladding layers.The cracking mechanism,microhardness,microstructure,phase composition,and wear properties were investigated.The relationship between thermal stress and the elastic and plastic fracture had been developed from the standpoint of fracture mechan-ics and thermal elastic fracture mechanics.The fracture criterion of the nickel-based coating was obtained,and the study has shown that the crack sensitivity could be reduced by decreasing the thermal expansion coefficientΔα.Thus,a new method was proposed,which the stainless steel nets were prefabricated on the substrate.It was found that the number of cracks reduced significantly with the addition of stainless steel net.When the stainless steel net with 14 mesh was added in Ni-based coatings,the average microhardness of nickel composite coating was 565 HV_(0.2),which was 2.6 times higher than that of the 45 steel substrate.Although the rare earth oxide 4 wt.%CeO_(2)and stainless steel net were added in the Ni-based coating reducing the microhardness(the average microhardness is 425 HV_(0.2)),the wear resistance of it improved substantially.The wear volume of Ni-based composite coating was 0.56×10^(−5) mm^(3)·N^(−1)·m^(−1),which was 85.1%lower than that of 45 steel.The experiment results have shown that the Nickel-based composite coating is equipped with low crack sensitivity and high abrasive resistance with austenitic stainless net and the rare earth oxide 4 wt.%CeO2.This research offers an efficient solution to produce components with low crack susceptibility and high wear-resistance coatings fabricated by laser cladding. | Zhenglei Yu Lunxiang Li Deqiang Zhang Guangfeng Shi Guang Yang Zezhou Xu Zhihui Zhang | 2021 | Chinese Journal of Mechanical Engineering2021，34，5： | 2 |
| 2 | Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica显示文摘Antarctic ice microalga can survive and thrive in channels or pores containing high salinity in Antarctic ice layer. In this study, it was found that cell membrane permeability of green microalga Chlamydomonas sp. L4 from Antarctic sea ice was high in cells treated with hypersalinity due to the induction of active oxygen and radicals. However, increased super oxide dismutase (SOD) scavenged harmful free radicals effectively to keep cell membrane integrity. Also, the analysis of membrane fatty acids demonstrated the content of saturated fatty acids and monounsaturated fatty acids increased and polyunsaturated fatty acids decreased under the high-salt treatment for 14 d, which effectively reduced the membrane fluidity and minimized the injury to cell membrane. The morpho- logical changes showed that hypersalinity induced the increase of cell volume and the consumption of starch granules. However, because of the increase in detoxification of vacuoles, electron-dense deposits and SOD activity under high-salt stress, the complete noninterference thylakoids, mitochondria and cell nucleus maintained cellular fundamental metabolism. Global-expression profiling of proteins showed eight protein spots disappeared, 18 protein spots decreased and 18 protein spots were enhanced after the high-salt shock obviously (P <0.05). One new peptide (pI 6.90; MW 51 kDa) was primarily confirmed as the processor of light reaction center protein CP43 in photosystem II, which increased photosynthesis ability of Chlamydomonas sp. L4 treated with high salinity. | KAN Guangfeng SHI Cuijuan WANG Xiaofei XIE Qiuju WANG Min WANG Xinlei MIAO Jinlai | 2012 | Acta Oceanologica Sinica2012，31，1： | 2 |
| 3 | The influence of core–shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi Huixuan Zhang | 2004 | European Polymer Journal2004，，11： | 1 |
| 4 | A randomized, multi-central, controlled study of patients with hepatitis B e antigen-positive chronic hepatitis B treated by adefovir dipivoxil or adefovir dipivoxil plus bicyclol显示文摘 | Wen Xie Guangfeng Shi Hongfei Zhang Guiming Zhao Zujiang Yu Zhenwei Lang Hong Zhao Jie Yan Jun Cheng | 2012 | Hepatology International2012，，2： | 1 |
| 5 | Strategy for the treatment of acute myelogenous leukemia based on folatereceptor-targeted liposomal doxorubicin combined with receptor induction using all-trans retinoic acid显示文摘 | Xuan Zheng Guangfeng Shi | 2002 | Blood2002，6，15： | 1 |
| 6 | Scutel-larein inhibits cancer cell metastasis in vitro and attenu-ates the development of fibrosarcoma in vivo显示文摘 | Shi Xiujuan Chen Guangfeng Liu Xiaoqiang | 2015 | Int J MolMed2015，，： | 1 |
| 7 | The influence of core–shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi Huixuan Zhang | 2004 | European Polymer Journal2004，，11： | 1 |
| 8 | Changes of proteins in the Antarctic ice microalga Chlamydomonas sp. cultured under UV-B radiation stress显示文摘Antarctic ice microalga Chlamydomonas sp. can thrive undisturbed under high UV radiation in the Antarctic ice layer. However, it is unknown that the initial adaptation mechanisms in protein level occurring in response to high UV radiation. Global-expression profiling of proteins in response to stress was analyzed by two-dimensional electrophoresis (2-DE) and image analysis. In the 2-DE analysis, protein preparation is the key step. Three different protein extract methods were compared, and the results showed that the trichloroacetic acid (TCA)-acetone fractional precipitation method was the fittest one. At the same time, the proteins in Chlamydomonas sp. were compared in 2-DE way, and the synthesis of seven protein spots was found disappeared and 18 decreased after exposed to UV-B radiation. In addition, 14 protein spots were enhanced or induced, among which two new peptides (20 and 21 kDa) appeared whose isoelectric point (pI) was 7.05 and 4.60 respectively. These changed proteins might act as key role in the acclimation of Antarctic ice microalga to UV-B | KAN Guangfeng MIAO Jinlai SHI Cuijuan LI Guangyou | 2006 | Acta Oceanologica Sinica2006，25，2： | 1 |
| 9 | Randomized, three‐arm study to optimize lamivudine efficacy in hepatitis B e antigen‐positive chronic hepatitis B patients显示文摘 | Xieer Liang Jun Cheng Yongtao Sun Xinyue Chen Tong Li Hao Wang Jianning Jiang Xiaoping Chen Hui Long Hong Tang Yanyan Yu Jifang Sheng Shijun Chen Junqi Niu Hong Ren Junping Shi Xiaoguang Dou Mobin Wan Jiaji Jiang Qing Xie Guangfeng Shi Qin Ning Chengwei C | 2015 | J Gastroenterol Hepatol2015，，4： | 1 |
| 10 | The influence of core-shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | WU Guangfeng ZHAO Junfeng SHI Haitao | 2004 | Eur Polym J2004，40，11： | 1 |
| 11 | Modeling,simulation and analysis of mechanical ruling echelle grating显示文摘 | SHI Guangfeng SHI Guoquan SONG Linsen | 2012 | Advanced Material Research2012，424，： | 1 |
| 12 | Epidemiology and vector efficiency during a dengue fever outbreak in Cixi, Zhejiang Province, China显示文摘 | Yang Tianci Lu Liang Fu Guiming Zhong Shi Ding Gangqiang Xu Rong Zhu Guangfeng Shi Nanfeng Fan Feilong Liu Qiyong | 2009 | Journal of Vector Ecology2009，，1： | 1 |
| 13 | The influence of core- shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi | 2004 | European Polymer Journal2004，40，： | 1 |
| 14 | lncRNA NEAT1 is required for splenic erythroid differentiation显示文摘The erythropoiesis process consists of differentiation stages,starting with erythroid progenitors,including erythroid burstforming unit cells(BFU-Es),erythroid colony-forming unit cells(CFU-Es),followed by terminal erythroid differentiation,which,in turn,gives rise to basophilic erythroblasts,polychromatic erythroblasts,orthochromatic erythroblasts,reticulocytes(RETs),and mature erythrocytes(Hu et al.,2013). | Xiaoru Zhang Yingnan Zhang Guangfeng Geng Jie Gao Jingyuan Tong Lihong Shi Jinhua Liu | 2023 | Journal of Genetics and Genomics2023，50，6： | 0 |
| 15 | Room Temperature 1.06-0.53 μm Second Harmonic Generation with MgO:LiNbO_(3)显示文摘Second harmonic generation conversion efficiency of 45%was obtained with a 12 mm long specially grown and cut MgO:LiNbO_(3) crystal and fundamental peak power density of 140MW/cm^(2). | YAO Jianquan SHI weiqiang J.E.Millerd XU Guangfeng E.Garmire M.Birnbaum | 1991 | Chinese Physics Letters1991，8，6： | 0 |
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