|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Schisandra sphenanthera extract(Wuzhi Tablet)protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway in mice显示文摘Alcohol abuse leads to alcoholic liver disease and no effective therapy is currently available.Wuzhi Tablet(WZ), a preparation of extract from Schisandra sphenanthera that is a traditional hepatoprotective herb, exerted a significant protective effect against acetaminophen-induced liver injury in our recent studies, but whether WZ can alleviate alcohol-induced toxicity remains unclear. This study aimed to investigate the contribution of WZ to alcohol-induced liver injury by using chronic-binge and acute models of alcohol feeding. The activities of ALT and AST in serum were assessed as well as the level of GSH and the activity of SOD in the liver. The expression of CYP2E1 and proteins in the NRF2-ARE signaling pathway including NRF2, GCLC, GCLM, HO-1 were measured, and the effect of WZ on NRF2 transcriptional activity was determined. We found that both models resulted in liver steatosis accompanied by increased transaminase activities, but that liver injury was significantly attenuated by WZ. WZ administration also inhibited CYP2E1 expression induced by alcohol, and elevated the level of GSH and the activity of SOD in the liver. Moreover, the NRF2-ARE signaling pathway was activated by WZ andthe target genes were all upregulated. Furthermore, WZ significantly activated NRF2 transcriptional activity. Collectively, our study demonstrates that WZ protected against alcohol-induced liver injury by reducing oxidative stress and improving antioxidant defense, possibly by activating the NRF2-ARE pathway. | Xuezhen Zeng Xi Li Chenshu Xu Fulin Jiang Yufei Mo Xiaomei Fan Yaoting Li Yiming Jiang Dongshun Li Min Huang Huichang Bi | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 13 |
| 2 | 3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration显示文摘Hydrogel scaffolds are attractive for tissue defect repair and reorganization because of their human tissue-like characteristics.However,most hydrogels offer limited cell growth and tissue formation ability due to their submicron-or nano-sized gel networks,which restrict the supply of oxygen,nutrients and inhibit the proliferation and differentiation of encapsulated cells.In recent years,3D printed hydrogels have shown great potential to overcome this problem by introducing macro-pores within scaffolds.In this study,we fabricated a macroporous hydrogel scaffold through horseradish peroxidase(HRP)-mediated crosslinking of silk fibroin(SF)and tyramine-substituted gelatin(GT)by extrusion-based low-temperature 3D printing.Through physicochemical characterization,we found that this hydrogel has excellent structural stability,suitable mechanical properties,and an adjustable degradation rate,thus satisfying the requirements for cartilage reconstruction.Cell suspension and aggregate seeding methods were developed to assess the inoculation efficiency of the hydrogel.Moreover,the chondrogenic differentiation of stem cells was explored.Stem cells in the hydrogel differentiated into hyaline cartilage when the cell aggregate seeding method was used and into fibrocartilage when the cell suspension was used.Finally,the effect of the hydrogel and stem cells were investigated in a rabbit cartilage defect model.After implantation for 12 and 16 weeks,histological evaluation of the sections was performed.We found that the enzymatic cross-linked and methanol treatment SF5GT15 hydrogel combined with cell aggregates promoted articular cartilage regeneration.In summary,this 3D printed macroporous SF-GT hydrogel combined with stem cell aggregates possesses excellent potential for application in cartilage tissue repair and regeneration. | Qingtao Li Sheng Xu Qi Feng Qiyuan Dai Longtao Yao Yichen Zhang Huichang Gao Hua Dong Dafu Chen Xiaodong Cao | 2021 | Bioactive Materials2021，6，10： | 6 |
| 3 | Lipidomics reveals carnitine palmitoyltransferase 1C protects cancer cells from lipotoxicity and senescence显示文摘Lipotoxicity, caused by intracellular lipid accumulation, accelerates the degenerative process of cellular senescence, which has implications in cancer development and therapy. Previously, carnitine palmitoyltransferase 1C(CPT1C), a mitochondrial enzyme that catalyzes carnitinylation of fatty acids, was found to be a critical regulator of cancer cell senescence. However, whether loss of CPT1C could induce senescence as a result of lipotoxicity remains unknown. An LC/MS-based lipidomic analysis of PANC-1,MDA-MB-231, HCT-116 and A549 cancer cells was conducted after siRNA depletion of CPT1C. Cellular lipotoxicity was further confirmed by lipotoxicity assays. Significant changes were found in the lipidome of CPT1C-depleted cells, including major alterations in fatty acid, diacylglycerol, triacylglycerol, oxidative lipids, cardiolipin, phosphatidylglycerol, phosphatidylcholine/phosphatidylethanolamine ratio and sphingomyelin. This was coincident with changes in expressions of mRNAs involved in lipogenesis.Histological and biochemical analyses revealed higher lipid accumulation and increased malondialdehyde and reactive oxygen species, signatures of lipid peroxidation and oxidative stress. Reduction of ATP synthesis, loss of mitochondrial transmembrane potential and down-regulation of expression of mitochondriogenesis gene m RNAs indicated mitochondrial dysfunction induced by lipotoxicity, which could further result in cellular senescence. Taken together, this study demonstrated CPT1C plays a critical role in the regulation of cancer cell lipotoxicity and cell senescence, suggesting that inhibition of CPT1C may serve as a new therapeutic strategy through induction of tumor lipotoxicity and senescence. | Huizhen Zhang Yongtao Wang Lihuan Guan Yixin Chen Panpan Chen Jiahong Sun Frank J.Gonzalez Min Huang Huichang Bi | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 3 |
| 4 | Hydrothermal growth of whitlockite coating on β-tricalcium phosphate surfaces for enhancing bone repair potential显示文摘In this study, we developed a simple approach for the controllable growth of whitlockite(WH) on a β-tricalcium phosphate surface and investigated its cell viability via CCK-8, its live-dead staining and its alkaline phosphatase activity. Herein, WH with controllable morphologies was prepared by regulating the hydrothermal reaction conditions. The results of scanning electron microscopy, X-ray diffraction and Xray photoelectron spectroscopy indicated that pure hexagonal plates of WH were prepared successfully.In vitro cell experiments showed that WH possessed excellent biocompatibility and effectively promoted the adhesion and proliferation of mouse bone mesenchymal stem cells. The osteogenesis of the WH was also enhanced. The obtained WH was expected to be utilized for promising applications as implantable block materials for bone repair. | Xiaoheng Guo Xiao Liu Huichang Gao Xuetao Shi Naru Zhao Yingjun Wang | 2018 | Journal of Materials Science & Technology2018，34，6： | 2 |
| 5 | Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation显示文摘The constitutive androstane receptor(CAR, NR3 I1) belongs to nuclear receptor superfamily.It was reported that CAR agonist TCPOBOP induces hepatomegaly but the underlying mechanism remains largely unknown. Yes-associated protein(YAP) is a potent regulator of organ size. The aim of this study is to explore the role of YAP in CAR activation-induced hepatomegaly and liver regeneration.TCPOBOP-induced CAR activation on hepatomegaly and liver regeneration was evaluated in wildtype(WT) mice, liver-specific YAP-deficient mice, and partial hepatectomy(PHx) mice. The results demonstrate that TCPOBOP can increase the liver-to-body weight ratio in wild-type mice and PHx mice.Hepatocytes enlargement around central vein(CV) area was observed, meanwhile hepatocytesproliferation was promoted as evidenced by the increased number of KI67+cells around portal vein(PV)area. The protein levels of YAP and its downstream targets were upregulated in TCPOBOP-treated mice and YAP translocation can be induced by CAR activation. Co-immunoprecipitation results suggested a potential proteineprotein interaction of CAR and YAP. However, CAR activation-induced hepatomegaly can still be observed in liver-specific YAP-deficient(Yape/e) mice. In summary, CAR activation promotes hepatomegaly and liver regeneration partially by inducing YAP translocation and interaction with YAP signaling pathway, which provides new insights to further understand the physiological functions of CAR. | Yue Gao Shicheng Fan Hua Li Yiming Jiang Xinpeng Yao Shuguang Zhu Xiao Yang Ruimin Wang Jianing Tian Frank JGonzalez Min Huang Huichang Bi | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 2 |
| 6 | Light weight, mechanically strong and biocompatible α-chitin aerogels from different aqueous alkali hydroxide/urea solutions显示文摘Light weight and mechanically strong α-chitin aerogels were fabricated using the sol-gel/self-assembly method from α-chitin in different aqueous alkali hydroxide(KOH, Na OH and Li OH)/urea solutions. All of the α-chitin solutions exhibited temperature-induced rapid gelation behavior. 13 C nuclear magnetic resonance(NMR) spectra revealed that the aqueous alkali hydroxide/urea solutions are non-derivatizing solvents for α-chitin. Fourier transform infrared(FT-IR), X-ray diffraction(XRD) and cross-polarization magic angle spinning(CP/MAS) 13 C NMR confirmed that α-chitin has a stable aggregate structure after undergoing dissolution and regeneration. Subsequently, nanostructured α-chitin aerogels were fabricated by regeneration from the chitin solutions in ethanol and then freeze-drying from t-Bu OH. These α-chitin aerogels exhibited high porosity(87% to 94%), low density(0.09 to 0.19 g/cm^3), high specific surface area(419 to 535 m^2/g) and large pore volume(2.7 to 3.8 cm^3/g). Moreover, the α-chitin aerogels exhibited good mechanical properties under compression and tension models. In vitro studies showed that m BMSCs cultured on chitin hydrogels have good biocompatibility. These nanostructured α-chitin aerogels may be useful for various applications, such as catalyst supports, carbon aerogel precursors and biomedical materials. | Beibei Ding Dan Zhao Jianhui Song Huichang Gao Duoduo Xu Min Xu Xiaodong Cao Lina Zhang Jie Cai | 2016 | Science China Chemistry2016，59，11： | 2 |
| 7 | Influence of 3D Microgrooves on C2C12 Cell Proliferation,Migration,Alignment,F-actin Protein Expression and Gene Expression显示文摘In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly(lactic-co-glycolic acid)(PLGA) substrate via combination of soft-lithography and melt-casting methods,and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstructure could affect cell behaviors in a much more extensive manner than what we had understood before. | Huichang Gao | 2016 | Journal of Materials Science & Technology2016，32，9： | 2 |
| 8 | Engineering topography: Effects on corneal cell behavior and integration into corneal tissue engineering显示文摘Cell-material interactions are important to tissue engineering.Inspired by the natural topographic structures on the extracellular matrix,a growing number of studies have integrated engineering topography into investigations of cell behavior on biomaterials.Engineering topography has a significant influence on cell behaviors.These cell-topography interactions play an important role in regenerative medicine and tissue engineering.Similarly,cell-topography interactions are important to corneal reconstruction and regeneration.In this review,we primarily summarized the effects of topographic cues on the behaviors of corneal cells,including cell morphology,adhesion,migration,and proliferation.Furthermore,the integration of engineering surface topography into corneal tissue engineering was also discussed. | Sijia Xiong HuiChang Gao Lanfeng Qin Yong-Guang Jia Li Ren | 2019 | Bioactive Materials2019，4，1： | 2 |
| 9 | Effects of flavonoids derived from Taxus yunnanensis on p-glycoprotein and cytochrome P450 3A4显示文摘The intestinal uptake of paclitaxel is hampered by trans-membrane efflux transporters such as P-glycoprotein(P-gp),and paclitaxel is mainly metabolized by cytochrome P4503A4(CYP3A4)presented in the liver.Our previous results demonstrated that flavonoids extracted from Taxus yunnanensis could improve the oral absorption of paclitaxel.The current study was purposed to investigate the effects of the flavonoid extracts on P-gp and CYP3A4 in vitro.The expression and activity of P-gp were detected by western blotting and intracellular rhodamine 123 accumulation assay in Caco-2 cells treated with the flavonoids extract.The expression of CYP3A4 was investigated by western blotting in mouse primary hepatocytes and the activity of CYP3A4 was detected by LC-MS/MS method using rat liver microsomes.Our results showed that the flavonoid extracts from T.yunnanensis could inhibit P-gp activity and concurrently decrease the expression and activity of CYP3A4.In conclusion,activity of P-gp and CYP3A4 could be inhibited by flavonoids extracted from T.yunnanensis which might be potential candidates for development of oral formulation of paclitaxel. | Jifu Li Dake Cai Huichang Bi Jing Jin Min Huang | 2013 | Asian Journal of Pharmaceutical Sciences2013，8，3： | 2 |
| 10 | Induction of Differentiation and apoptosis by Ligands of poroxisone proliferartion-activated receptor in nonsmall cell lung cancer显示文摘 | Szabo EVa | 2000 | Cancer Researeh2000，15，2： | 1 |
| 11 | The Role of Parents' Control in Early Adolescents' Psychological Functioning: a Longitudinal Investigation in the United States and China 显示文摘 | Qian Wang Eva M Pomerantz Huichang Chen | 2007 | Child Development2007，78，5： | 1 |
| 12 | The modeling and analysis for autonomous navigation system based on tightly coupled GPS/1NS显示文摘 | WANG Lijun YANG Xiaoniu ZHAO Huichang | 2006 | International Conference on Microwave and Millimeter Wave Technology2006，，： | 1 |
| 13 | Optimizing the drying parameters of a fixed bed with reversing ventilation for peanut using computer simulation显示文摘To obtain the optimal operation parameters of fixed-bed reversing ventilation drying of peanuts,a set of partial differential equations indicating the heat and mass transfer relationships between the peanut pods and air during drying was proposed.Then,a series of discretized models were established for simulation,and the time consumed,unevenness,and energy consumption for batch drying were calculated.The results showed that reversing ventilation and segmented drying was helpful to these issues for high drying ability.The optimal operation parameters were determined by uniform design experimentation of mathematical simulation.The result showed that when the moisture content(wet basis)was above 22%,a ventilation velocity of 0.46 m/s was optimal;when the moisture content was between 8%and 22%,a ventilation velocity of 0.20 m/s was optimal.Using the optimal parameters,the computer simulating result was compared with the experimental results.The correlation coefficients between the simulating and the experimental values for the temperature and moisture content were all above 0.98 and the quality of dried peanuts was close to that of natural sun-dried ones,which indicates that the optimization results of the drying parameters are highly reliable. | Jianchun Yan Huanxiong Xie Hai Wei Huichang Wu Zhaoyan You | 2021 | International Journal of Agricultural and Biological Engineering2021，14，5： | 1 |
| 14 | YAP regulates the liver size during the fasting-refeeding transition in mice显示文摘Liver is the central hub regulating energy metabolism during feeding-fasting transition.Evidence suggests that fasting and refeeding induce dynamic changes in liver size,but the underlying mechanisms remain unclear.Yes-associated protein(YAP)is a key regulator of organ size.This study aims to explore the role of YAP in fasting-and refeeding-induced changes in liver size.Here,fasting significantly reduced liver size,which was recovered to the normal level after refeeding.Moreover,hepatocyte size was decreased and hepatocyte proliferation was inhibited after fasting.Conversely,refeeding promoted hepatocyte enlargement and proliferation compared to fasted state.Mechanistically,fasting or refeeding regulated the expression of YAP and its downstream targets,as well as the proliferation-related protein cyclin D1(CCND1).Furthermore,fasting significantly reduced the liver size in AAV-control mice,which was mitigated in AAV Yap(5SA)mice.Yap overexpression also prevented the effect of fasting on hepatocyte size and proliferation.Besides,the recovery of liver size after refeeding was delayed in AAV Yap shRNA mice.Yap knockdown attenuated refeeding-induced hepatocyte enlargement and proliferation.In summary,this study demonstrated that YAP plays an important role in dynamic changes of liver size during fasting-refeeding transition,which provides new evidence for YAP in regulating liver size under energy stress. | Xuan Li Shicheng Fan Chenghui Cai Yue Gao Xinhui Wang Yifei Zhang Hangfei Liang Huilin Li Jie Yang Min Huang Huichang Bi | 2023 | Acta Pharmaceutica Sinica B2023，13，4： | 1 |
| 15 | A comparative investigation on urban radio noise at several specific measured areas and its applications for communications显示文摘 | Ming Huichang Ken Huanglin | 2004 | IEEE Transactions on Broadcasting2004，50，3： | 1 |
| 16 | Origami meets electrospinning:a new strategy for 3D nanofiber scaffolds显示文摘Inspired by the constitution of things in the natural world,three-dimensional (3D)nanofiber scaffold/cells complex was constructed via the combination of electrospinning technology and origami techniques.The nanofiber boxes prepared by origami provided a limited space for the layer-by-layer nanofiber films,and the human fetal osteoblasts (hFOBs)seeded on the both sides of the nanofiber films were expected to facilitate the bonding,of the adjacent nanofiber films through the secretion of extracellular matrix.Specifically,the hFOBs presented 3D distribution in the nanofiber scaffold,and they can stretch across the gaps between the adjacent nanofiber films,forming the cell layers and filling the whole 3D nanofiber scaffold.Eventually,a 3D block composed of electrospun nanofiber scaffold and cells was obtained',which possesses potential applications in bone tissue engineering.Interestingly,we also created 3D nanofiber structures that range from simple forms to intricate architectures via origami,indicating that the combination of electrospinning technology and origami techniques is a feasible method for the 3D construction of tissue engineering scaffolds. | Juqing Song Guanglin Zhu Huichang Gao Lin Wang Nanying Li Xuetao Shi Yingjun Wang | 2018 | Bio-Design and Manufacturing2018，1，4： | 1 |
| 17 | Experiment and parameter optimization of an automatic row following system for the traction beet combine harvester显示文摘To improve the automation level and operation quality of China's beet harvester and reduce the loss due to damaged and missed excavation,this study used a self-developed sugar beet combine harvester and field simulation experiment platform,based on the single-factor bench test of the automatic row following system in the early stage,taking hydraulic flow A,spring preload B,and forward speed C which have significant influence on performance indices as test factors,and taking the missed excavation rate,breakage rate and reaction time as performance indices,the orthogonal experimental study on the parameter optimization of the three-factor and three-level automatic row following system with the first-order interaction of various factors was carried out.The results of the orthogonal experiments were analyzed using range analysis and variance analysis.The results showed that there were differences in the influence degree,factor priority order and first-order interaction,and the optimal parameter combination on each performance index.A weighted comprehensive scoring method was used to optimize and analyze each index.The optimal parameter combination of the overall operating performance of the automatic row following system was A 2B 2C 1,that is,the hydraulic flow was 25 L/min,the forward speed was 0.8 m/s,and the spring preload was 198 N.Under this combination,the response time was 0.496 s,the missed excavation rate was 2.35%,the breakage rate was 3.65%,and the operation quality was relatively good,which can meet the harvest requirements.The comprehensive optimization results were verified by field experiments with different ridge shapes and different planting patterns.The results showed that the mean values of the missed excavation rate of different planting patterns of conventional straight ridges and extremely large'S'ridges were 2.23%and 2.69%,respectively,and the maximum values were 2.39%and 2.98%,respectively;the average damage rates were 3.38%and 4.14%,and the maximum values were 3.58%and 4.48%,which meet the industry standards of sugar beet harvester operation quality.The overall adaptability of the automatic row following system is good.This study can provide a reference for research on automatic row following harvesting systems of sugar beets and other subsoil crop harvesters. | Shenying Wang Xuemei Gao Zhaoyan You Baoliang Peng Huichang Wu Zhichao Hu Yongwei Wang | 2023 | International Journal of Agricultural and Biological Engineering2023，16，1： | 1 |
| 18 | Hepato-protective effects of six schisandra lignans on acetaminophen-induced liver injury are partially associated with the inhibition of CYP-mediated bioactivation显示文摘 | Yiming Jiang Xiaomei Fan Ying Wang Huasen Tan Pan Chen Hang Zeng Min Huang Huichang Bi | 2015 | Chemico-Biological Interactions2015，，： | 1 |
| 19 | Small molecule-based immunomodulators for cancer therapy显示文摘Immunotherapy has led to a paradigm shift in the treatment of cancer.Current cancer immunotherapies are mostly antibody-based,thus possessing advantages in regard to pharmacodynamics(e.g.,specificity and efficacy).However,they have limitations in terms of pharmacokinetics including long half-lives,poor tissue/tumor penetration,and little/no oral bioavailability.In addition,therapeutic antibodies are immunogenic,thus may cause unwanted adverse effects.Therefore,researchers have shifted their efforts towards the development of small molecule-based cancer immunotherapy,as small molecules may overcome the above disadvantages associated with antibodies.Further,small molecule-based immunomodulators and therapeutic antibodies are complementary modalities for cancer treatment,and may be combined to elicit synergistic effects.Recent years have witnessed the rapid development of small molecule-based cancer immunotherapy.In this review,we describe the current progress in small molecule-based immunomodulators(inhibitors/agonists/degraders)for cancer therapy,including those targeting PD-1/PD-L1,chemokine receptors,stimulator of interferon genes(STING),Toll-like receptor(TLR),etc.The tumorigenesis mechanism of various targets and their respective modulators that have entered clinical trials are also summarized. | Yinrong Wu Zichao Yang Kui Cheng Huichang Bi Jianjun Chen | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 1 |
| 20 | 'One-flask' transformation of isocyanates and isothiocyanates to guanidines hydrochloride by using sodium bis(trimethylsilyl) amide显示文摘 | Chen Chunyen Lin Huichang Huang Yuying | 2010 | Tetrahedron2010，66，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员