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| 1 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 2 | Mini-review of application of IoT technology in monitoring agricultural products quality and safety显示文摘Internet of Things(IoT)technology has been identified as one of the emerging technologies in information technology(IT),which is widely used in all walks of life in the world.The key technologies of IoT technology,such as radio frequency identification(RFID),wireless sensor network(WSN),sensors and global positioning system(GPS),had been widely used in farmland and greenhouse management,environmental monitoring,cold chain monitoring and tracking,and traceability,etc.As advancements in science and technology,IoT technology has been widely applied in agricultural products quality and safety,which brought a number of potential benefits for detecting agricultural products easier and automatically,improving the efficiency and speed of operations,decreasing the labor cost,promoting the agricultural products quality and safety.This paper intended to introduce the concept of IoT technology,the key technologies used at present and review the recent applications of IoT technology in monitoring agricultural products quality and safety in the whole industry chain,such as production,processing,circulation,sales and traceability.Challenges and trends of agricultural products quality and safety in the future were also discussed. | Hua Ping Jihua Wang Zhihong Ma Yuanfang Du | 2018 | International Journal of Agricultural and Biological Engineering2018，11，5： | 4 |
| 3 | cAMP Modulates Macrophage Development by Suppressing M-CSF-Induced MAPKs Activation显示文摘M-CSF is a key cytokine in macrophage development by inducing MAPKs activation,and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development,the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay,and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis. Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK,JNK and p38 in both mature and immature macrophages,and cAMP inhibited M-CSF-induced ERK,JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion,cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation. | Ning Zhu Jian Cui Chunxia Qiao Yan Li Yuanfang Ma Jiyan Zhang Beifen Shen | 2008 | Cellular & Molecular Immunology2008，5，2： | 4 |
| 4 | Characterization of a Novel Anti-DR5 Monoclonal Antibody WD1 with the Potential to Induce Tumor Cell Apoptosis显示文摘TNF-related apoptosis-inducing ligand (TRAIL) is a TNF family member capable of inducing apoptosis. Death receptor 5 (DR 5) is a key receptor of TRAIL and plays an important role in TRAIL-induced apoptosis. To prepare monoclonal antibodies (mAbs) against DR5,cDNA encoding soluble DR5 (sDR5) was firstly amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with specific primers,and then inserted into a prokaryotic expression vector pET-30a. The recombinant plasmid was expressed in Escherichia coli strain BL21 (DE3),and sDR5 was purified by nickel affinity chromatography. As an antigen,sDR5 was used to immunize mice. Hybridomas secreting antibodies against sDR5 were identified. One positive clone was selected to produce antibody,WD1. ELISA and immunofluorescence demonstrated that WD1 could bind recombinant sDR5 and membrane-bound DR5 (mDR5) on Jurkat and Molt-4 cells. ATPLite assays showed that Jurkat and Molt-4 cells were sensitive to the antibody in a dose dependent manner. The Annexin V/PI assays and Giemsa's staining both showed that WD1 could induce Jurkat cell apoptosis efficiently. Transient transfection of 293T cells and indirect immunofluorescence assay demonstrated that mAb (WD1) couldn't cross-react with DR4. Our findings indicated that the novel antibody,WD1 could act as a direct agonist,bind DR5 characteristically,and initiate efficient apoptotic signaling and tumor regression. Thus,WD1 would be a leading candidate for potential cancer therapeutics. | Jing Wang Zhou Lin Chunxia Qiao Ming Lv Ming Yu He Xiao Qingyang Wang Liyan Wang Jiannan Feng Beifen Shen Yuanfang Ma Yan Li | 2008 | Cellular & Molecular Immunology2008，5，1： | 4 |
| 5 | Ginger-indirect moxibustion plus acupuncture versus acupuncture alone for chronic fatigue syndrome:a randomized controlled trial显示文摘OBJECTIVE:To assess the efficacy and safety of gingerindirect moxibustion for chronic fatigue syndrome(CFS).METHODS:In this central randomized,controlled trial,290 CFS participants were recruited and randomly allocated to group A(ginger-indirect moxibustion plus acupuncture)or group B(acupuncture alone).The study consisted of a treatment period of 8 weeks with a total of 24 treatments(3 sessions per week,every other day),and a follow-up period of 12 weeks.The outcome was measured by Fatigue Severity Scale(FSS),Psychological Health Report(SPHERE),the Self-rating depression scale(SDS)and the Hamilton anxiety scale(HAMA)at baseline,2,4,6,8,12 and 20 weeks.RESULTS:With the treatment undergoing,the changes of FSS,SPHERE,SDS and HAMA scores in both groups increased gradually,and the effect maintained at the 12th week.Between groups,significantly higher score changes were seen in group A in FSS after 4 weeks treatment(11.94 vs 9.12,95%CI:0.94,4.7)and in SPHERE after 2 weeks treatment(3.7 vs 2.27,95%CI:0.56,2.31).But for SDS and HAMA,the improvement did not differ significantly between groups.No severe adverse events were reported.CONCLUSION:Ginger-indirect moxibustion is a safe and effective intervention to relieve fatigue and accompanying physical symptoms of CFS. | MA Tingting WU Jie YANG Lijie FENG Fen YANG Huilin ZHANG Jinhua ZHONG Yanjin NING Qing HUANG Lirong LIN Youbing YAN Jue CHEN Guiquan HOU Tianshu WANG Li REN Yuanfang TAN Jing | 2022 | Journal of Traditional Chinese Medicine2022，42，2： | 2 |
| 6 | Analysis of TRAIL receptor expression using a novel anti - TRAIL death receptor 5 monoclonal antibody 显示文摘 | Ma Yuanfang Chen Yiguang Chen Youhai | 2003 | CMJ2003，16，： | 1 |
| 7 | Analysis of TRAIL receptor expression using a novel anti-TRAIL death receptor5 monoclonal antibody 显示文摘 | Ma Yuanfang Chen Yiguang Chen Youhai | 2003 | CMJ2003，16，： | 1 |
| 8 | Serum proteomic-based analysis for the identification of a potential serological marker for autoimmune hepatitis显示文摘 | LU Feng XIA Qing MA Yuanfang | 2008 | Biochem Biophys Res Commun2008，367，2： | 1 |
| 9 | Structural Model of Ni/Graphite/BaTiO3 Composites显示文摘 | Juan Du Yuanfang Qu Weibing Ma | 2004 | Journal of Electroceramics2004，，3： | 1 |
| 10 | High-Affinity Decoy PD-1 Mutant Screened from an Epitope-Specific Cell Library显示文摘Immunotherapy with anti-programmed cell death protein-1(PD-1)/programmed cell death ligand-1(PDL1)monoclonal antibodies has become routine in the treatment of many kinds of human cancers,such as lung cancer,intestinal cancer,and melanoma.The PD-1/PD-L1 pathway inhibits T cell activation in the micro-environment,making it an attractive target against cancer.Wild-type(WT)PD-1 ectodomain has been shown to have difficulty blocking PD-1/PD-L1 mixture formation due to its low affinity.The present work uses three-dimensional(3D)crystal complex structures to analyze the interaction by which PD-1 binds to PD-L1 or PD-L2.It also reports on a theoretical study of the binding mode between PD-1 and its clinical antibody Opdivo.Based on the theoretical binding analysis of PD-1 and its ligands(i.e.,PD-L1 and PD-L2)or antibody(Opdivo),a small-content,epitope-oriented mammalian cell library was established for PD-1.After three rounds of cell sorting,the decoy PD-1 mutant 463,which presented a higher affinity than WT PD-1 to the PD-L1(the affinity has increased by almost three orders of magnitude)was screened out.It exhibited an inhibitory effect against PD-1 to prevent it from forming mixtures with PD-L1,which was similar to the effect of the commercial anti-PD-L1 antibody atezolizumab(ATE).The median effective concentration(EC50)value of the decoy mutant was 0.031 μg·mL^(-1) in comparison with 0.063 μg·mL^(-1) for ATE;both values were much lower than that of WT PD-1,at 2.571 μg·mL^(-1).The 463 decoy mutant reversed the inhibitory function of PD-1 in T cell activation;furthermore,10 mg·kg^(-1) of 463 inhibited about 75%of tumor growth in vivo in a MC38 transgenic xenograft mice model,which was similar to the activity of ATE.More interestingly,an even lower dose of 463(2 mg·kg^(-1))showed a better effect than 10 mg·kg^(-1) of WT PD-1.This work offers the decoy 463 with an improved curative effect,which holds potential to become a good option against PD-1/PD-L1-related cancers. | Hao Liu Chunxia Qiao Naijing Hu Zhihong Wang Jing Wang Jiannan Feng Beifen Shen Yuanfang Ma Longlong Luo | 2021 | Engineering2021，7，11： | 0 |
| 11 | Effects of transient temperature gradient on frost heave of saturated silty clay in an open system显示文摘Frost heave in seasonally frozen regions is a one-dimensional process that could severely damage infrastructure subgrades.Stress state,temperature and water migration are important factors for frost heave.This work investigated the effects of soil temperature and volumetric water content on the transient frost heave ratio during the freezing of saturated silty clay in an open system and analyzed the relationships between the transient frost heave ratio and freezing rate and between temperature gradient and frost heave rate.The results show that the frost heave ratio,frost heave rate,and freezing rate are positively correlated with the temperature gradient since the temperature gradient drives the water migration during freezing,indicating the transient temperature gradient could be used to evaluate the frost heave of saturated silty clay.The transient freezing rate and transient frost heave ratio are logarithmically related to the transient frost heave ratio and transient temperature gradient,respectively.The effects of transient temperature gradient on frost heave are the principal mechanism responsible for different frost heave characteristics and uneven frost heave along a subgrade of the same soil type. | HongYan Ma YuanFang Cui JianQiao Zhang Song Li Song Xu | 2023 | Research in Cold and Arid Regions2023，15，6： | 0 |
| 12 | 维生素E琥珀酸酯增强DR5抗体对Raji和K562细胞的凋亡作用(英文)显示文摘Objective: The aim of our study was to detect whether Vitamin E Succinic Acid (VES) could regulate the expression level of DR5 in the cells and further elucidate the potential mechanisms involving that VES enhances the effect of mDRA-6 to eradicate leukemia Raji and K562 cells. Methods: MTT method was used to detect the growth inhibition of VES and mDRA-6 to Raji and K562 cells. Annexin V-FITC/PI double staining assay was used to analysis the apoptosis of leukemia cell. Flow cytometry was used to detect the cell surface DR5 expression. Immunoblotting technique was used to detect the DR5 protein expression. Results: MTT detection showed that 10 μmol/L mDRA-6 on the cell death rates of Raji and K562 cells were 21.98% and 5.23%, respectively. While increasing concentration of VES (5 μmol/L, 10 μmol/L, 20 μmol/L) and mDRA-6 both on the cell viability of Raji or K562 cells, the mortality of Raji cells elevated to 24.67%, 35.65% (P<0.01) and 40.22% (P<0.01), respectively. Similarly, the mortality of K562 cells increase to 6%, 7.89% (P<0.01) and 8.67% (P<0.01), respectively. To further specify the increased cell death rate induced by mDRA-6 and VES, the treated cells were analyzed by Annexin-V/PI staining assay. As shown in Fig. 1, the apoptosis rates of Raji and K562 cells treated with 2 μg/mL mDRA-6 for 12 h were 20.79% and 7.74%. Compared with this, the proportion of apoptotic cells increased upon exposure to 2 μg/mL mDRA-6 combination with 10 μmol/L VES, the apoptosis rates of Raji and K562 cells were 43.18% and 16.99%, respectively. To examine the anticancer effects of a combination strategy based on mDRA-6 and VES. We analyzed whether VES could elevated the expression level of DR5 on Raji and K562 cytomembrane by FACS. Interestingly, after treated with 10 μmol/L VES for 12 h, the expression level of DR5 on Raji and K562 cell surface increased from 50.66% to 70.08%, and 15.02% to 16.38%, respectively. Immune imprinting technology test showed that, different concentrations of VES could increase Raji and K562 cell DR5 protein expression. Conclusion: VES enhances the effect of mDRA-6 to eradicate leukemia Raji and K562 cells. The proper mechanism is VES could enhance the Raji and K562 cell membrane expression of DR5, and VES can also enhance the DR5 protein expression of cells. | Jun Zhang Shulian Li Jingfang Du Yuanfang Ma | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，4： | 0 |
| 13 | mDRA-6与尼美舒利对人肝癌细胞系SMMC-7721细胞的协同杀伤效应(英文)显示文摘Objective: The aim of this work was to evaluate anti-tumor effects of mDRA-6 plus nimesulide on a human hepatocellular cancer cell line, SMMC-7721, and study the main mechanisms. Methods: The DR5 receptor of SMMC-7721 cells was detected by flow cytometry (FCM). For further experimental application, SMMC-7721 cells were treated with proper dose of mDRA-6, nimesulide, or mDRA-6 plus 200 μmol/L nimesulide; untreated SMMC-7721 cells were comparably set as control. Cytotoxicity was tested by MTT assay; cell morphology was examined using Hoechst 33258 staining; and apoptosis was determined by FCM. Results: The positive rate of DR5 on SMMC-7721 was 95.0%. Either mDRA-6 or nimesulide alone induces SMMC-7721 cell death in a dose-dependent manner. Treatment of 1,600 ng/mL mDRA-6 for 12h led to a cell-death rate of 35.0%, while an increased cell-death rate (91.1%) was found under the same condition of mDRA-6 treatment supple- mented with 200 μmol/L nimesulide. Hoechst 33258 and Annexin V/PI staining confirmed apoptosis as the main cause of this anti-tumor response. Conclusion: Both mDRA-6 and nimesulide can induce apoptosis of SMMC-7721 cells, and they have synergistic anti-tumor activities against SMMC-7721. | Jun Zhang Yingjie Liu Zengyi Ma Jing wang Shulian Li Huiling Bai Yuanfang Ma | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，12： | 0 |
| 14 | Functional Identification of the Stable Transfection C5aR Cell Line Molt-4显示文摘The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Gαi and Gα16.C5aR is mostly expressed on neutrophils,macrophages and endothelial cells.C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage.Considering the limitation of collection of human peripheral blood neutrophils and their short half life,the stably transfected cell line for studying the biological effects of C5aR is needed.In this study,we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR.Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERK1/2 phosphorylation,Ca ++ influx.This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR. | Chunmei Zhang Ruonan Xu Jianan Wang Gencheng Han Guojiang Chen Renxi Wang Huawei Wei Beifen Shen Yuanfang Ma Yan Li | 2007 | Cellular & Molecular Immunology2007，4，6： | 0 |
| 15 | Preface显示文摘 | Tao Xie Yuanfang Cai Xuanzhe Liu Xiaoyin Wang Mithun P. Acharya Marcelo d'Amorim Xiaoxing Ma | 2017 | Journal of Computer Science & Technology2017，32，6： | 0 |
| 16 | Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its mechanism显示文摘The aim of this study is to explore inhibitory activity of Bifidobacterium adolescent combined with cis-platin on the growth of melanoma(B16)in mice and the underlying mechanism.C57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated with bifidobacterium adolescent combined with cisplatin.Ratios of inhibitory activity on the growth of melanoma(B16)were calculated.Pathology changes of the tumor were observed by HE staining.B16 cell cycles were examined on a flow cytometer.Lymphocyte prolif-eration was measured with MTT assay and the T-cell sub-set was measured by double marked fluorescence.When bifidobacterium of 1010 cfu/L was injected,the ratio of inhibitory activity on the growth of melanoma(B16)reached 54%,which was similar to that of cisplatin group.The ratio of inhibitory activity reached 74.45%when the mice were treated by bifidobacterium combined with cis-platin.HE staining shows that bifidobacterium inhibited B16 cell proliferation and enhanced the cisplatin`s killing activity on B16 cells.The results of flow cytometry demonstrated that B16 cell proliferation was arrested at G1 stage after treatment with bifidobacterium.The B16 cell proliferation was arrested at S stage after treatment with cisplatin.The CD4+percentage increased and the difference was significant compared with the normal group after treatment with bifidobacterium,indicating that T-cell immune activity was enhanced.Treatment with bifidobacterium combined with cisplatin can enhance the inhibitory activity on the growth of melanoma(B16)of cisplatin.The mechanism of the inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice. | Hongying HUANG Guangchao LIU Yijun QI Yaowu DU Jugao CHEN Yuanfang MA | 2008 | Frontiers of Medicine2008，2，2： | 0 |
| 17 | Author Correction:Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prog nosis in esophageal squamous cell carcinoma显示文摘Correction to:Cell Research https://doi.org/10.1038/s41422-020-0333-6,published online 12 May 2020 In the initial published version of this article,there was an error in the Supplementary information,Table S1.Row 463 and row 80 have the same sample id BDESCC-01-838.After checking the original id of these two samples,we found that they were actually two different samples.This error was caused by Excel random numbering when we assigned the external id to each sample.Thus,we re-assigned a new id BDESCC-01-839 to the row 4P3 sample to fix this issue. | Yongping Cui Hongyan Chen Ruibin Xie Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qi min Zhan Yanhong Li Zhihua Liu | 2022 | Cell Research2022，32，4： | 0 |
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