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| 1 | Wx^lv、the Ancestral Allele of Rice Waxy Gene显示文摘In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. | Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu | 2019 | Molecular Plant2019，12，8： | 22 |
| 2 | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency显示文摘In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs. | Changquan Zhang Yong Yang Shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan Shuzhu Tang Minghong Gu Qiaoquan Liu | 2021 | Journal of Integrative Plant Biology2021，63，5： | 18 |
| 3 | The X-linked mental retardation gene PHF8 is a histone demethylase involved in neuronal differentiation显示文摘最近的研究在 PHF8 识别了变化,编码 JmjC 的 X 连接基因包含域的蛋白质,为 X 连接智力迟钝(XLMR ) 的一个原因的因素并且劈开 lip/cleft 腭。然而,内在的机制是未知的。这里,我们证明 PHF8 为 retinoic 酸受体(RAR ) 是 histone demethylase 和激活剂。尽管为 H3K4me3/2/1 和 H3K9me2/1 demethylation 的活动在细胞底的试金被检测, recombinant PHF8 在 vitro 展出了仅仅 H3K9me2/1 demethylase 活动,建议 PHF8 是其特性可以在 vivo 被调制的 H3K9me2/1 demethylase。重要地,在 XLMR 病人识别的变异的 PHF8 (在到丝氨酸的位置 279 点的本氨基丙酸) 在酶的活动是有缺点的,显示 histone demethylase 活动的损失有原因地与疾病的发作被连接。另外,我们证明 PHF8 经由一个 N 终端哲学博士手指领域明确地绑在 H3K4me3/2 肽。与为在 neuronal 区别的 PHF8 的一个角色一致,在鼠标的 PHF8 击倒胚胎的癌 P19 房间损害导致 RA 的 neuronal 区别,而 overexpression 野类型然而并非向 neuronal 区别的 F279S 变异的 PHF8 驱动器 P19 房间。而且,我们证明 PHF8 为 RAR 伪作为激活剂与 RAR 伪和功能交往。一起拿,我们的结果建议 PHF8 调制的 histone methylation 在 neuronal 区别起一个关键作用。 | Jihui Qiu Guang Shi Yuanhui Jia Jing Li Meng Wu Jiwen Li Shuo Dong Jiemin Wong | 2010 | Cell Research2010，20，8： | 16 |
| 4 | Structure of the YTH domain of human YTHDF2 in complex with an m6A mononucleotide reveals an aromatic cage for m6A recognition显示文摘 | Fudong Li Debiao Zhao Jihui Wu Yunyu Shi | 2014 | Cell Research2014，24，12： | 16 |
| 5 | Self-microemulsifying drug delivery system for improving the bioavailability of huperzine A by lymphatic uptake显示文摘Huperzine A(Hup-A) is a poorly water-soluble drug with low oral bioavailability. A selfmicroemulsifying drug delivery system(SMEDDS) was used to enhance the oral bioavailability and lymphatic uptake and transport of Hup-A. A single-pass intestinal perfusion(SPIP) technique and a chylomicron flow-blocking approach were used to study its intestinal absorption, mesenteric lymph node distribution and intestinal lymphatic uptake. The value of the area under the plasma concentration–time curve(AUC) of Hup-A SMEDDS was significantly higher than that of a Hup-A suspension(P <0.01).The absorption rate constant(K_a) and the apparent permeability coefficient(P_(app)) for Hup-A in different parts of the intestine suggested a passive transport mechanism, and the values of K_a and P_(app) of Hup-A SMEDDS in the ileum were much higher than those in other intestinal segments. The determination of Hup-A concentration in mesenteric lymph nodes can be used to explain the intestinal lymphatic absorption of Hup-A SMEDDS. For Hup-A SMEDDS, the values of AUC and maximum plasma concentration(C_(max)) of the blocking model were significantly lower than those of the control model(P<0.05). The proportion of lymphatic transport of Hup-A SMEDDS and Hup-A suspension were about 40% and 5%,respectively, suggesting that SMEDDS can significantly improve the intestinal lymphatic uptake and transport of Hup-A. | Fang Li Rongfeng Hu Bin Wang Yun Gui Gang Cheng Song Gao Lei Ye Jihui Tang | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 15 |
| 6 | PGC-1α induces apoptosis in human epithelial ovarian cancer cells through a PPARy-dependent pathway显示文摘Peroxisome 激活 proliferator 的受体鲸鱼群妈(PPAR γ) coactivator-1 高山哈(PGC-1 α) coactivates 多重抄写因素并且调整几个代谢过程。Thecurrent 学习在人的上皮的卵巢的癌症房间在 apoptosis 的正式就职调查了 PGC-1 α的角色。在人的卵巢和人的卵巢的上皮的肿瘤之间的 PGC-1 α信使 rna 水平被量的 RT-PCR 检验。更少的 PGC-1 α表示与正常卵巢相比在恶意的肿瘤的表面上皮被发现。在人的上皮的卵巢的癌症房间线 Ho-8910 的 PGC-1 α的 Overexpression 通过 Bcl-2 和 Bax 表示的协调规定导致了房间 apoptosis。Microarray 分析证实 PGC-1 α戏剧性地在 Ho-8910 房间影响了 apoptosis 相关的基因。 Mitochondrial 功能的试金证明 apoptosis 的正式就职通过由细胞色素 c.Furthermore 的版本的终端阶段,导致的 apoptosis 部分是,然而并非完全,由 PPAR γa ntagonist ( GW9662 )堵住了的 PGC-1 α-,并且由 siRNA 的 PPAR γ 表示的抑制也在 Ho-8910 房间禁止了 PGC-1 α- inducedapoptosis 。这些数据建议 PGC-1 α通过 aPPAR γ - 依赖者小径施加了它的效果。我们的调查结果显示 PGC-1 α涉及 apoptotic signaltransduction 小径, PGC-1 α的 down 规定可以是在支持上皮的卵巢的癌症生长和前进的一个关键点。 | Yan Zhang Yi Ba Chang Liu Guoxun Sun Li Ding Songyuan Gao Jihui Hao Zhentao Yu Junfeng Zhang Ke Zen Zhongsheng Tong Yang Xiang Chen-Yu Zhang | 2007 | Cell Research2007，17，4： | 14 |
| 7 | Association of biochar properties with changes in soil bacterial,fungal and fauna communities and nutrient cycling processes显示文摘Soil microorganisms play crucial roles in soil nutrient cycling,carbon sequestration,fertility maintenance and crop health and production.To date,the responses of microorganisms,such as microbial activity,diversity,community structure and nutrient cycling processes,to biochar addition have been widely reported.However,the relationships between soil microbial groups(bacteria,fungi and microscopic fauna)and biochar physicochemical properties have not been summarized.In this review,we conclude that biochar affects soil microbial growth,diversity and community compositions by directly provid-ing growth promoters for soil biota or indirectly changing soil basic properties.The porous structure,labile C,high pH and electrochemical properties of biochar play an important role in determining soil microbial abundance and communities,and their mediated N and P cycling processes,while the effects and underlying mechanisms vary with biochar types that are affected by pyrolysis temperature and feedstock type.Finally,we highlight some issues related to research methodology and subjects that are still poorly understood or controversial,and the perspectives for further research in microbial responses to biochar addition. | Zhongmin Dai Xinquan Xiong Hang Zhu Haojie Xu Peng Leng Jihui Li CTang Jianming Xu | 2021 | Biochar2021，3，3： | 12 |
| 8 | PD-L1 is a direct target of cancer-FOXP3 in pancreatic ductal adenocarcinoma(PDAC),and combined immunotherapy with antibodies against PD-L1 and CCL5 is effective in the treatment of PDAC显示文摘High expression of PD-L1 marks the poor prognosis of pancreatic ductal adenocarcinomas(PDAC).However,the regulatory mechanism of PD-L1 remains elusive.We recently reported that cancer Forkhead box protein 3(Cancer-FOXP3 or C-FOXP3)promoted immune evasion of PDAC by recruiting Treg cells into PDAC via upregulation of CCL5.In this study,we confirmed that PD-L1 was overexpressed in PDAC samples from two independent cohorts of patients with radical resection.Moreover,C-FOXP3 was colocalized and correlated with the expression of PD-L1 in tumor cells at the mRNA and protein levels,and this finding was confirmed by the The Cancer Genome Atlas(TCGA)database.Chromatin immunoprecipitation(ChIP)revealed that C-FOXP3 directly bound to the promoter region of PD-L1 in pancreatic cancer cells.Furthermore,overexpression of C-FOXP3 activated the luciferase reporter gene under the control of the PD-L1 promoter.However,mutation of the binding motif-a completely reversed the luciferase activity.In addition,C-FOXP3-induced upregulation of PD-L1 effectively inhibited the activity of CD8+T cells.Based on our recent finding that the CCL-5 antibody achieved a better response to PDAC models with high C-FOXP3 levels,we further demonstrated that the PD-L1 antibody strengthened the antitumor effect of CCL-5 blockade in xenograft and orthotopic mouse models with high C-FOXP3 levels.In conclusion,C-FOXP3 directly activates PD-L1 and represents a core transcription factor that mediates the immune escape of PDAC.Combined blockade of PD-L1 and CCL-5 may provide an effective therapy for patients with PDAC that have high C-FOXP3 levels. | Xiuchao Wang Xin Li Xunbin Wei Haiping Jiang Chungen Lan Shengyu Yang Han Wang Yanhui Yang Caijuan Tian Zanmei Xu Jiangyan Zhang Jihui Hao He Ren | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 11 |
| 9 | Artificial intelligence in drug design显示文摘Thanks to the fast improvement of the computing power and the rapid development of the computational chemistry and biology,the computer-aided drug design techniques have been successfully applied in almost every stage of the drug discovery and development pipeline to speed up the process of research and reduce the cost and risk related to preclinical and clinical trials.Owing to the development of machine learning theory and the accumulation of pharmacological data, the artificial intelligence(AI) technology, as a powerful data mining tool, has cut a figure in various fields of the drug design, such as virtual screening,activity scoring, quantitative structure-activity relationship(QSAR) analysis, de novo drug design, and in silico evaluation of absorption, distribution, metabolism, excretion and toxicity(ADME/T) properties. Although it is still challenging to provide a physical explanation of the AI-based models, it indeed has been acting as a great power to help manipulating the drug discovery through the versatile frameworks. Recently, due to the strong generalization ability and powerful feature extraction capability,deep learning methods have been employed in predicting the molecular properties as well as generating the desired molecules,which will further promote the application of AI technologies in the field of drug design. | Feisheng Zhong Jing Xing Xutong Li Xiaohong Liu Zunyun Fu Zhaoping Xiong Dong Lu Xiaolong Wu Jihui Zhao Xiaoqin Tan Fei Li Xiaomin Luo Zhaojun Li Kaixian Chen Mingyue Zheng Hualiang Jiang | 2018 | Science China(Life Sciences)2018，61，10： | 10 |
| 10 | Important role of a LAL regulator Sta R in the staurosporine biosynthesis and high-production of Streptomyces fradiae CGMCC 4.576显示文摘Staurosporine, belonging to indolocarbazole compounds, is regarded as an excellent lead compound for synthesizing antitumor agents as a potent inhibitor against various protein kinases. In this study, two separate clusters(cluster A and cluster B),corresponding to biosyntheses of K-252 c(staurosporine aglycone) and sugar moiety, were identified in Streptomyces fradiae CGMCC 4.576 and heterologously expressed in Streptomyces coelicolor M1146 separately or together. Sta R, a cluster-situated LAL family regulator, activates staurosporine biosynthesis by binding to the promoter regions of sta O-sta C and sta G-sta N. The conserved sequences GGGGG and GCGCG were found through gradually truncating promoters of sta O and sta G, and further determined by mutational experiments. Overexpression of sta R with the supplementation of 0.01 g L^–1 Fe SO4 increased staurosporine production to 5.2-fold compared with that of the parental strain Streptomyces fradiae CGMCC 4.576 in GYM medium. Our results provided an approach for improvement of staurosporine production mediated by a positive regulator and established the basis for dissecting the regulatory mechanisms of other indolocarbazole compounds with clinical application value. | Hanye Guan Yue Li Jiazhen Zheng Ning Liu Jihui Zhang Huarong Tan | 2019 | Science China(Life Sciences)2019，62，12： | 6 |
| 11 | Quantitative nanoscale mapping of three-phase thermal conductivities in filled skutterudites via scanning thermal microscopy显示文摘In the last two decades, a nano structuring paradigm has been successfully applied in a wide range of thermoelectric materials, resulting in significant reduction in thermal conductivity and superior thermoelectric performance. These advances, however, have been accomplished without directly investigating the local thermoelectric properties, even though local electric current can be mapped with high spatial resolution. In fact, there still lacks an effective method that links the macroscopic thermoelectric performance to the local micro structures and properties. Here, we show that local thermal conductivity can be mapped quantitatively with good accuracy, nanometer resolution and one-to-one correspondence to the micro structure using a three-phase skutterudite as a model system. Scanning thermal microscopy combined with finite element simulations demonstrate close correlation between sample conductivity and probe resistance,enabling us to distinguish thermal conductivities spanning orders of magnitude, yet resolving thermal variation across a phase interface with small contrast. The technique thus provides a powerful tool to correlate local thermal conductivities, micro structures and macroscopic properties for nano structured materials in general and nano structured thermo electrics in particular. | ehsan nasr esfahani feiyue ma shanyu wang yun ou jihui yang jiangyu li | 2018 | National Science Review2018，5，1： | 6 |
| 12 | A new combined criterion to better predict malignant lesions in patients with pancreatic cystic neoplasms显示文摘Objective:Cystic lesions of the pancreas have been increasingly recognized.Some lesions exhibit benign behavior,while others have unequivocal malignant potential.Thus,accurate identification of malignancy in patients diagnosed with pancreatic cystic neoplasms(PCNs)remains a major challenge.The aim of this study was to define a combined criterion to better predict malignant lesions in patients with PCNs.Methods:We retrospectively analyzed 165 patients who underwent resection of PCNs from October 2011 to May 2017.The relationship among malignancy and serum carbohydrate antigen 19-9(CA19-9),preoperative neutrophil-to-lymphocyte ratio(NLR),and the presence of enhanced solid component on imaging was analyzed.Results:NLR before surgery in patients with malignant PCNs(2.81±2.14)was significantly higher than that in patients diagnosed with pancreatic neuroendocrine tumor(1.90±0.69,P=0.013)or healthy volunteers(1.40±0.48;P<0.001).Serum CA19-9≥39U/m L,NLR>1.976 and presence of enhanced solid component were independent predictors of PCN malignancy.A combined criterion meeting any two or more of the three elements including CA19-9≥39 U/m L,NLR>1.976,and presence of enhanced solid component on computed tomography imaging is an indicator with a high positive predictive value of 80.5%and a high negative predictive value of 87.9%,and thus,represents a highly accurate test(86.1%).Conclusions:The new combined criterion is an effective predictor of tumor malignancy in patients with PCNs. | Chungen Lan Xin Li Xiuchao Wang Jihui Hao He Ren | 2018 | Cancer Biology & Medicine2018，15，1： | 6 |
| 13 | Enhancement of salinomycin production by ribosome engineering in Streptomyces albus显示文摘Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bacteria are spreading at alarming rates. | Dong Li Jihui Zhang Yuqing Tian Huarong Tan | 2019 | Science China(Life Sciences)2019，62，2： | 5 |
| 14 | Efficacy of moxibustion by stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) on hyperplasia of mammary gland in rats显示文摘OBJECTIVE: To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) in rats with hyperplasia of mammary gland(HMG) which induced by estrogen and progestogen.METHODS: Thirty female Sprague-Dawley rats were randomly divided into saline control group,HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progesto-gen with moxibustion at the same time. The Changes of nipple diameter and height were measured.The rats' skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.RESULTS: The diameter and height of the nipples in model group were prominently bigger and higher than that in control group(P < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group(P < 0.01), and there was no significant difference between moxibustion group and control group.Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong(CV 17) and Ganshu(BL 18) decreased prominently in model group(P < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently(P <0.05).CONCLUSION: Treatment with moxibustion can effectively decrease the HMG rats' nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia. | Yang Jiamin Wu Jihui Shen Xiaoyu Zhang Mei Liu Zhenzhen Qi Dandan Zhou Jin Cui Xiao Zhang Lufen Li Yuhang | 2018 | Journal of Traditional Chinese Medicine2018，38，1： | 3 |
| 15 | Possibility of women treated with fertility-sparing surgery fornon-epithelial ovarian tumors to safely and successfully becomepregnant--a Chinese retrospective cohort study among 148cases显示文摘This study was performed to evaluate the oncological and reproductive outcomes of childbearing-agewomen treated with fertility-sparing surgery (FSS) for non-epithelial ovarian tumors in China. One hundred andforty eight non-epithelial ovarian tumor women treated with FSS between January I, 2000 and August 31, 2015from two medical centers in China were identified. Progression-free survival (PFS) was 88.5%, whereas overallsurvival (OS) was 93.9%. Univariate analysis suggested that delivery after treatment is related to PFS (P = 0.023),whereas histology significantly influenced OS. Cox regression analysis suggested that only histology was associatedwith PFS and OS (P 〈 0.05). Among the 129 women who completed adjuvant chemotherapy (ACT), nonedeveloped amenorrhea. Among the 44 women who desired pregnancy, 35 (79.5%) successfully had 51 gestationsincluding 35 live births without birth defects. Non-epithelial ovarian tumors can achieve fulfilling prognosis afterFSS and chemotherapy. Histology might be the only independent prognostic factor for PFS and OS. FSS followedby ACT appeared to have little or no effect on fertility. Meanwhile, postoperative pregnancy did not increase thePFS or OS. Use of gonadotropin-releasing hormone agonist was not beneficial for fertility. | Bin Yang Yan Yu Jing Chen Yan Zhang Ye Yin Nan Yu Ge Chen Shifei Zhu Haiyan Huang Yongqun Yuan Jihui Ai Xinyu Wang Kezhen Li | 2018 | Frontiers of Medicine2018，12，5： | 3 |
| 16 | Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality显示文摘在小囊的液体的自由激进分子的活动与卵巢的应答有关,在 vitro 授精(IVF ) ,和胚胎转移成功评价。然而,学习与卵巢的症候群(PCOS ) 正在缺乏的 polycystic 分析在免费激进的清除能力和不肥沃的女人的胚胎质量之间的关系。这研究的目的是与 PCOS 和他们的胚胎评估在女人的免费激进的清除窗户之间的关系优秀的。从有 PCOS 的女人的小囊的液体的免费激进的清除能力被决定由一, a-diphenyl-b-picrylhydrazyl (DPPH ) , 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic 酸) 试金, superoxide 基,和反应的氧种类(ROS ) 试金。在 DPPH 和 ROS 试金,从等级的小囊的液体我和 II 胚胎是比从等级 III 和 IV 胚胎的小囊的液体显著地高级的。激进的清除能力和 ROS 的上面的控制限制铺平的 DPPH 的更低的控制限制分别地是 13.2% 和 109.0 每秒字符数。计算更低的控制限制和上面的控制限制进一步在所有等级的胚胎的小囊的液体被证实。小囊的液体的免费激进的清除活动的这些截止价值能在在经历 IVF 的 PCOS 病人选择胚胎的发展帮助胚胎学家。 | Bo Huang Zhou Li Xinling Ren Jihui Ai Lixia Zhu Lei Jin | 2017 | Frontiers of Medicine2017，11，2： | 2 |
| 17 | Novel nanometer scaffolds regulate the biological behaviors of neural stem cells显示文摘Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber scaffolds were prepared using electronic spinning technology. Their diameters and appearance reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The proportions of cells in the S and G 2 /M phases noticeably increased. Moreover, the proliferation rate of neural stem cells on the aligned collagen nanofiber scaffolds was high. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds. These results indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression. | Jihui Zhou Fuge Sui Meng Yao Yansong Wang Yugang Liu Feipeng Tian Qiang Li Xiaofeng He Lin Shao Zhiqiang Liu | 2013 | Neural Regeneration Research2013，8，16： | 2 |
| 18 | Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants显示文摘Cytocompatibility of high nitrogen nickel-free stainless steel(HNS) with different nitrogen content was evaluated and compared with a conventional austenitic stainless steel 317L.The MTT assay(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was performed on MG63 osteoblasts to assess the cytotoxicity.The expression of selected marker typical of differentiated osteoblasts,such as alkaline phosphatase activity(AKP),was also monitored in MG63 cells cultured on the tested materials.As a result,HNS had higher cell growth than 317L;meanwhile the cytocompatibility was increased with increasing nitrogen content.Furthermore,HNS enhanced osteoblasts differentiation,as confirmed by AKP activity.Overall these facts indicated that HNS had higher cytocompatibility than 317L and the nitrogen content contributed to the higher cytocompatibility of HNS.The influence of nitrogen on surface energy further explained the cytocompatibility of HNS. | Tianchi Ma Peng Wan Yuyan Cui Guirong Zhang Jiqiang Li Jihui Liu Yibin Ren Ke Yangg Li Lu | 2012 | Journal of Materials Science & Technology2012，28，7： | 2 |
| 19 | Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo transfers显示文摘 | Ye Yin Ge Chen Kezhen Li Qiuyue Liao Sijia Zhang Nieying Ma Jing Chen Yan Zhang Jihui Ai | 2017 | Frontiers of Medicine2017，11，4： | 2 |
| 20 | A widespread response of Gram-negative bacterial acyl-homoserine lactone receptors to Gram-positive Streptomyces γ-butyrolactone signaling molecules显示文摘Cell-cell communication is critical for bacterial survival in natural habitats,in which miscellaneous regulatory networks are encompassed.However,elucidating the interaction networks of a microbial community has been hindered by the population complexity.This study reveals thatγ-butyrolactone(GBL)molecules from Streptomyces species,the major antibiotic producers,can directly bind to the acyl-homoserine lactone(AHL)receptor of Chromobacterium violaceum and influence violacein production controlled by the quorum sensing(QS)system.Subsequently,the widespread responses of more Gram-negative bacterial AHL receptors to Gram-positive Streptomyces signaling molecules are unveiled.Based on the cross-talk between GBL and AHL signaling systems,combinatorial regulatory circuits(CRC)are designed and proved to be workable in Escherichia coli(E.coli).It is significant that the QS systems of Gram-positive and Gram-negative bacteria can be bridged via native Streptomyces signaling molecules.These findings pave a new path for unlocking the comprehensive cell-cell communications in microbial communities and facilitate the exploitation of innovative regulatory elements for synthetic biology. | Xiang Liu Wenxi Wang Junyue Li Yue Li Jihui Zhang Huarong Tan | 2021 | Science China(Life Sciences)2021，64，10： | 2 |
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