|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cloned goats (Gapra hircus) from foetal fibroblast cell lines显示文摘Mammalian cloning has been one of the most active research topics in the world. Cioning with in vitro culured foetal fibroblast cells, in comparison with embryonic cells, can be used not only to theoretically study the embryonic or cellular development and differentiation in mammals, but also to utilize the unlimited fibroblast cells to produce large numbers of clonings. The preliminary results are as follows: (i) The division and development of the cloned embryos with embryonic donor cells and goat foetal fibroblast donor cells were 55%, 77% and 35%, 31%, respectively. There is no significant statistical difference between them. (? These studies result in the birth of two cloned goats derived from two 30-day foetal fibroblast celi lines, which are the first cloned mammals from somatic cells in China. This project has established a technological data base for the furture research on adult mammalian somatic cloning and nucleocytoplasmic interactions in animal development, and a novel technique for the | WANG Yuge ZOU Xiangang LIU Jie ZHANG Jingpu ZHANG Xuechen LAO Weide DU Miao CHENG Guoxing CHENG Yong CHEN Jianquan ZHANG Suolin XU Shaofu | 2000 | Chinese Science Bulletin2000，45，1： | 4 |
| 2 | Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing,China显示文摘The objective of this study was to investigate the genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii clinical isolates from Beijing,China.173 A.baumannii clinical isolates from hospitals in Beijing from 2006 to 2009 were first subjected to high level aminoglycoside resistance(HLAR,MIC to gentamicin and amikacin>512 mg/mL)phenotype selection by broth microdilution method.The strains were then subjected to genetic basis analysis by PCR detection of the aminoglycoside modifying enzyme genes(aac(3)-Ⅰ,aac(3)-Ⅱc,aac(60)-Ⅰb,aac(60)-Ⅱ,aph(4)-Ⅰa,aph(30)-Ⅰ,aph(30)-Ⅱb,aph(30)-Ⅲa,aph(30)-Ⅵa,aph(2″)-Ⅰb,aph(2″)-Ⅰc,aph(2″)-Ⅰd,ant(2″)-Ⅰa,ant(3″)-Ⅰand ant(40)-Ⅰa)and the 16S rRNA methylase genes(armA,rmtB and rmtC).Correlation analysis between the presence of aminoglycoside resistance gene and HLAR phenotype were performed by SPSS.Totally 102(58.96%)HLAR isolates were selected.The HLAR rates for year 2006,2007,2008 and 2009 were 52.63%,65.22%,51.11%and 70.83%,respectively.Five modifying enzyme genes(aac(3)-Ⅰ,detection rate of 65.69%;aac(60)-Ⅰb,detection rate of 45.10%;aph(30)-Ⅰ,detection rate of 47.06%;aph(30)-Ⅱb,detection rate of 0.98%;ant(3″)-Ⅰ,detection rate of 95.10%)and one methylase gene(armA,detection rate of 98.04%)were detected in the 102 A.baumannii with aac(3)-Ⅰ+aac(60)-Ⅰ+þant(3″)-Ⅰ+armA(detection rate of 25.49%),aac(3)-Ⅰ+aph(30)-Ⅰ+ant(3″)-Ⅰ+armA(detection rate of 21.57%)and ant(3″)-Ⅰ+armA(detection rate of 12.75%)being the most prevalent gene profiles.The values of chi-square tests showed correlation of armA,ant(3″)-Ⅰ,aac(3)-Ⅰ,aph(30)-Ⅰand aac(60)-Ⅰb with HLAR.armA had significant correlation(contingency coefficient 0.685)and good contingency with HLAR(kappa 0.940).The high rates of HLAR may cause a serious problem for combination therapy of aminoglycoside with β-lactams against A.baumannii infections.As armA was reported to be able to cause high level aminoglycoside resistance to most of the clinical important aminoglycosides(gentamicin,amikacin,tobramycin,etc),the function of aminoglycoside modifying enzyme gene(s)in A.baumannii carrying armA deserves further investigation. | Lu Nie Yuemeng Lv Min Yuan Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Jing Pang Jingpu Zhang Congran Li Xiukun Wang Xuefu You | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 3 |
| 3 | Application of a new wind driving force model in soil wind erosion area of northern China显示文摘The shear stress generated by the wind on the land surface is the driving force that results in the wind erosion of the soil.It is an independent factor influencing soil wind erosion.The factors related to wind erosivity,known as submodels,mainly include the weather factor(WF)in revised wind erosion equation(RWEQ),the erosion submodel(ES)in wind erosion prediction system(WEPS),as well as the drift potential(DP)in wind energy environmental assessment.However,the essential factors of WF and ES contain wind,soil characteristics and surface coverings,which therefore results in the interdependence between WF or ES and other factors(e.g.,soil erodible factor)in soil erosion models.Considering that DP is a relative indicator of the wind energy environment and does not have the value of expressing wind to induce shear stress on the surface.Therefore,a new factor is needed to express accurately wind erosivity.Based on the theoretical basis that the soil loss by wind erosion(Q)is proportional to the shear stress of the wind on the soil surface,a new model of wind driving force(WDF)was established,which expresses the potential capacity of wind to drive soil mass in per unit area and a period of time.Through the calculations in the typical area,the WDF,WF and DP are compared and analyzed from the theoretical basis,construction goal,problem-solving ability and typical area application;the spatial distribution of soil wind erosion intensity was concurrently compared with the spatial distributions of the WDF,WF and DP values in the typical area.The results indicate that the WDF is better to reflect the potential capacity of wind erosivity than WF and DP,and that the WDF model is a good model with universal applicability and can be logically incorporated into the soil wind erosion models. | ZOU Xueyong LI Huiru LIU Wei WANG Jingpu CHENG Hong WU Xiaoxu ZHANG Chunlai KANG Liqiang | 2020 | Journal of Arid Land2020，12，3： | 2 |
| 4 | Human iPS Cells Loaded with MnO2-Based Nanoprobes for Photodynamic and Simultaneous Enhanced Immunotherapy Against Cancer显示文摘How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodynamic therapy(PDT)and enhanced immunotherapy.Ce6 photosensitizer was attached on manganese dioxide(MnO2)nanoparticles,and resultant MnO2@Ce6 nanoprobes were delivered into mitomycin-treated iPSs to form iPS-MnO2@Ce6 nanoprobes.The iPS-MnO2@Ce6 actively targeted in vivo tumors,the acidic microenvironment triggered interaction between MnO2 and H2O2,released large quantities of oxygen,alleviated hypoxia in tumor.Upon PDT,singlet oxygen formed,broken iPSs released tumor-shared antigens,which evoked an intensive innate and adaptive immune response against the tumor,improving dendritic cells matured,effector T cells,and natural killer cells were activated.Meanwhile,regulatory T cells were reduced,and then the immune response induced by iPS-MnO2@Ce6 was markedly stronger than the immune reaction induced by MnO2@Ce6(P<0.05).The iPS-MnO2@Ce6 markedly inhibited tumor growth and metastasis and reduced mortality in mice models with tumor.Human iPS s loaded with MnO2-based nanoprobes are a promising strategy for simultaneous PDT and enhanced immunotherapy against tumor and own clinical translational prospect. | Yanlei Liu Jingxing Yang Bin Liu Wen Cao Jingpu Zhang Yuming Yang Lijun Ma Jesus Martinez de la Fuente Jie Song Jian Ni Chunfu Zhang Daxiang Cui | 2020 | Nano-Micro Letters2020，12，10： | 2 |
| 5 | FLNa negatively regulated proliferation and metastasis in lung adenocarcinoma A549 cells via suppression of EGFR显示文摘Filamin A (FLNa ) 是无所不在地表示的细胞质的蛋白质,它 24 次象 Ig 一样重复跟随的一个 N 终端肌动朊绑定领域(ABD ) 创作。FLNa 作为连接 transmembrane 受体的细胞骨架蛋白质工作,包括 integrins,到 F 肌动朊并且用作发信号的中介。最近的研究作为与超过 90 蛋白质交往并且在细胞的发信号的 transduction 起重要作用的支架蛋白质识别了 FLNa。在人的 FLNa 基因的变化或缺点被显示了引起众多的发展缺点。而且, FLNa 的异常表示在许多癌症被观察了例如甲状旁腺的肿瘤,颈的癌症,和乳癌。然而,它在肺腺癌的角色很少被讨论了。在现在的学习,我们的在里面 vitro 并且在 vivo,研究证明在肺癌症房间线 A549 房间的那 silencing FLNa 表情由提高表皮的生长因素受体和英皇家空军之阶级最低之兵发信号小径的激活支持了 A549 房间的增长,移植,和侵略。这些结果在肺癌症和揭开的新奇机制使 FLNa 的新奇功能清楚些,这些结果为肺腺癌为预言和治疗提供了可能的目标。 | Yuna Zhang Tienian Zhu Jingpu Liu Jiankun Liu Dongmei Gao Tongyi Su Ruijing Zhao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 2 |
| 6 | Co-expression of multiple gene constructs in transgenic mice显示文摘 | Jingpu Zhang Yuge Wang Guangsan Li Yingyun Wei Guofa Hu Yu Shen Miao Du | 2001 | Biotechnology Letters2001，，15： | 1 |
| 7 | Laser rapid melting structure and property for Ni-based tungsten carbide alloy显示文摘 | Zhang Song Kang Yuping Zhu Jingpu | 1996 | Chinese Journal of Laser1996，5，4： | 1 |
| 8 | Desertification and Blown Sand Disaster in China显示文摘 | Yanli Lyu Yanyan Yang Lanlan Guo Lianyou Liu Peijun Shi Guoming Zhang Zhiqiang Qu Xia Hu Jingpu Wang Yiying Xiong Haiming Wen Jie Lei Bo Liang Jiadong Dai | 2016 | Journal of Agricultural Science and Technology(A)2016，6，6： | 1 |
| 9 | Laser Rapid Meting Structure and Property for Ni-Based Tungsten Carbide Alloy显示文摘 | Zhang Song Kang Yuping Zhu Jingpu | 1996 | Chinese Journal of Laser1996，5，4： | 1 |
| 10 | Application of a nuclear localization signal gene in transgene mice显示文摘Efficient gene transfer by cytoplasm co-injec-tion will offer a powerful means for transgenic animals. Us-ing co-injection in cytoplasm, two independent gene con-structs, including bovine a-s1-casein-hG-CSF and a mammal expression vector expressing a nuclear localization signal (mNLS), were introduced into fertilized mouse eggs. The target gene construct was docked into host nucleus probably by the nuclear localization signal. Transgene mice have been obtained at 58% (29/50) of integration ratio. Ex-pression level of the positive transgene mice was detected by Western blotting. Maximal expression of human G-CSF was estimated about 540 mg/L of milk. The expression ratio was up to 75% (9/12). The results here have important practical implications for the generation of mammary gland bioreac-tors and other transgene studies. Co-injection of a target gene with an expression vector of a mammal nuclear localization signal by cytoplasm appears to be a useful, efficient and easy strategy for generating | Jingpu Zhang Yuge Wang Guangsan Li Yu Shen Shaoxia Zhu Miao Du | 2002 | Chinese Science Bulletin2002，47，3： | 1 |
| 11 | 302 W triple-frequency,single-mode,linearly polarized Yb-doped all-fiber amplifier显示文摘Stimulated Brillouin scattering(SBS) effect is currently the major limitation for the power scaling of singlefrequency/narrow linewidth fiber laser systems. A single-mode linearly polarized all-fiber amplifier system is set up to investigate SBS effect in triple-frequency high-power amplifiers. With this amplifier, up to 302 W output power with 83% slope efficiency is achieved and the SBS threshold is scaled up to 12 d B. To the best of our knowledge, this is the highest output power of multifrequency laser from a single-mode polarization maintaining fiber. Good spectral properties and high brightness make this laser source available for the application of second harmonic generation, coherent beam combining. | Xiang Zhao Yifeng Yang Hui Shen Xiaolong Chen Gang Bai Jingpu Zhang Yunfeng Qi Bing He Jun Zhou | 2017 | High Power Laser Science and Engineering2017，5，4： | 0 |
| 12 | Evolution and development of potent monobactam sulfonate candidate IMBZ18g as a dual inhibitor against MDR Gram-negative bacteria producing ESBLs显示文摘A series of new monobactam sulfonates is continuously synthesized and evaluated for their antimicrobial efficacies against Gram-negative bacteria.Compound 33a(IMBZ18G)is highly effective in vitro and in vivo against clinically intractable multi-drug-resistant(MDR)Gram-negative strains,with a highly druglike nature.The checkerboard assay reveals its significant synergistic effect withβ-lactamase inhibitor avibactam,and the MIC values against MDR enterobacteria were reduced up to 4—512folds.X-ray co-crystal and chemoproteomic assays indicate that the anti-MDR bacteria effect of 33a results from the dual inhibition of the common PBP3 and some class A and Cβ-lactamases.Accordingly,preclinical studies of 33a alone and 33a-avibactam combination as potential innovative candidates are actively going on,in the treatment ofβ-lactamase-producing MDR Gram-negative bacterial infections. | Zhiwen Li Zhihao Guo Xi Lu Xican Ma Xiukun Wang Rui Zhang Xinxin Hu Yanxiang Wang Jing Pang Tianyun Fan Yonghua Liu Sheng Tang Haigen Fu Jingpu Zhang Yinghong Li Xuefu You Danqing Song | 2023 | Acta Pharmaceutica Sinica B2023，13，7： | 0 |
| 13 | Cloning and roles of goldfish maternal factor β-Catenin cDNA in embryonic development显示文摘Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and func-tions in this paper. We have cloned goldfish b-Catenin cDNA gene and compared it with zebrafish b-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern of b-Catenin by in situ hybridization and the roles of b-Catenin on embryonic de-velopment by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that b-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of b-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposte-rior axes, and leads embryos to die before larva get hatched. | ZHANG Jingpu1,2,WANG Weixian1 & ZHU Shaoxia1 1.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China 2.The Graduate School of the Chinese Academy of Sciences,Beijing 100039,China Correspondence should be addressed to Zhang Jingpu | 2004 | Chinese Science Bulletin2004，49，7： | 0 |
| 14 | Discovery and development of tricyclic matrinic derivatives as anti-diabetic candidates by AMPKαactivation显示文摘We found compound 12N-p-trifluoromethylbenzenesulfonyl matrinane(1)was a potent anti-diabetic agent.Thirty-five tricyclic matrinic derivatives were synthesized and determined for their stimulatory effects on glucose consumption in L6 myotubes,taking 1 as the lead.In high-fat diet(HFD)and STZ induced diabetic mice,9a significantly lowers blood glucose,improves glucose tolerance,and especially alleviates diabetic nephropathy and islet damage.Mechanism study indicates that 9a simultaneously targets mitochondrial complex I to increase AMP/ATP ratio,as well as liver kinase B1(LKB1)and calcium/calmodulindependent protein kinase(Ca MKK),which synergistically activates AMPKαand then stimulates glucose transporter 4(GLUT4)membrane translocation and 2-deoxyglucose(2-DG)uptake to exert anti-diabetic efficacy.Therefore,compound 9a with a novel structure is a promising anti-diabetic candidate with the advantage of multiple-target mechanism,worthy of further investigation. | Yinghong Li Yuanhui Zhang Tianyu Niu Yudong Pang Yulong Shi Qingxuan Zeng Jingpu Zhang Jingyang Zhu Xiuli Zhong Yanxiang Wang Yan Wang Sheng Tang Weijia Kong Danqing Song Jiandong Jiang | 2023 | Chinese Chemical Letters2023，34，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员