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| 1 | Antitumor activity of fucoidan against diffuse large B cell lymphoma in vitro and in vivo显示文摘Fucoidan 是从棕色的海草孤立的主要 sulfated 多糖之一。在这研究,我们在弥漫的大 B 房间淋巴瘤(DLBCL ) 上决定了 fucoidan 的反癌症活动在 vitro 并且在 vivo 的房间。Fucoidan 在一个剂量依赖者和时间依赖者举止禁止了 DLBCL 细胞的生长,并且 fucoidan 治疗挑起了 G 0/G1 细胞周期拘捕,它被 p21 伴随起来规定和 cyclin D1, Cdk4,和 Cdk6 下面规定。Fucoidan 也在 DLBCL 房间线和主要 DLBCL 房间导致了 caspase 依赖的房间 apoptosis。另外, fucoidan 治疗从线粒体引起了 mitochondrial 膜潜力的损失和细胞色素 c 和导致 apoptosis 因素的版本进 cytosol。Fucoidan 也加强了在杀死 DLBCL 房间的 carfilzomib 的活动。fucoidan 的口头的管理有效地在异种皮移植老鼠模型禁止了肿瘤生长。我们的调查结果作为一个 anti-DLBCL 代理人揭示 fucoidan 的新奇功能,它能在 DLBCL 的临床的治疗被使用。 | Guang Yang Qianqiao Zhang Yuanyuan Kong Bingqian Xie Minjie Gao Yi Tao Hongwei Xu Fenghuang Zhan Bojie Dai Jumei Shi Xiaosong Wu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 9 |
| 2 | Resveratrol induces AMPK and mTOR signaling inhibition-mediated autophagy and apoptosis in multiple myeloma cells显示文摘Resveratrol,a natural compound extracted from the skins of grapes,berries,or other fruits,has been shown to have anti-tumor effects against multiple myeloma(MM)via promoting apoptosis and inhibiting cell viability.In addition to apoptosis,autophagy also plays a significant role in anti-tumor effects.However,whether autophagy is involved in anti-MM activity of resveratrol remains unclear.In this study,human MM cell lines U266,RPMI-8226,and NCI-H929 were treated with resveratrol.Cell Counting Kit-8 assay and colony formation assay were used to measure cell viability.Western blot analysis was used to detect apoptosis-and autophagy-associated proteins.3-Methyladenine(3-MA)was applied to inhibit autophagy.Results showed that resveratrol inhibited cell viability and colony formation via promoting apoptosis and autophagy in MM cell lines U266,RPMI-8226,and NCI-H929.Resveratrol promoted apoptosis-related proteins,Caspase-3 activating poly-ADP-ribose polymerase and Caspase-3 cleavage,and decreased the protein level of Survivin in a dose-dependent manner.Additionally,resveratrol upregulated the levels of LC3 and Beclin1 in a dose-dependent way,indicating that autophagy might be implicated in anti-MM effect of resveratrol.Furthermore,3-MA relieved the cytotoxicity of resveratrol by blocking the autophagic flux.Resveratrol increased the phosphorylation of adenosine monophosphate(AMP)-activated protein kinase and decreased the phosphorylation of mammalian target of rapamycin(mTOR)and its downstream substrates p70S6K and 4EBP1 in a dose-dependent manner,leading to autophagy.Therefore,our results suggest that resveratrol exerts anti-MM effects through apoptosis and autophagy,which can be used as a new therapeutic strategy for MM in clinic. | Ruye Ma Dandan Yu Yu Peng Hongfei Yi Yingcong Wang Taofang Cheng Bingqing Shi Guang Yang Weiming Lai Xiaosong Wu Ye Lu Jumei Shi | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 7 |
| 3 | Dihydrocelastrol inhibits multiple myeloma cell proliferation and promotes apoptosis through ERK1/2 and IL-6/STAT3 pathways in vitro and in vivo显示文摘多重骨髓瘤(公里) 是第二很经常的恶意的 hematological 疾病。Dihydrocelastrol (DHCE ) 被 hydrogenated celastrol 综合,从中国药用的植物 Tripterygium regelii 孤立的 treterpene。在这研究,我们首先在公里房间上报导了 DHCE 的反肿瘤活动。我们发现 DHCE 能禁止房间增长并且在 vitro 通过 caspase 依赖的方法支持 apoptosis。另外, DHCE 能使 interleukin (IL ) 的表达式失去活性 -6 和 downregulate 细胞外的调整蛋白质 kinases (ERK1/2 ) 和信号变换器和在公里的抄写 3 的使活跃之物(STAT3 ) 的 phosphorylation。它也面对 IL-6 对公里房间线保留了它的活动。而且,有 DHCE 的公里房间的处理在房间周期的 G 0/G1 阶段导致了房间的累积。尤其是, DHCE 减少了 4 和 6 在公里房间衬里的 cyclin D1 和 cyclin 依赖的 kinases 的表示。另外,它向 MM 房间线的功效能与 histone deacetylase 禁止者 panobinostat (LBH589 ) 在联合被提高,它在公里作为潜在的治疗学的策略暗示了 DHCE 和 LBH589 的联合处理的可能性。另外,有 DHCE 的 NCI-H929 忍受肿瘤的裸体老鼠的处理(10 mg/kg/d, i.p, 1-14 天) 在 vivo 导致了肿瘤生长的 73% 抑制。一起拿,我们的现在的学习的结果显示 DHCE 能禁止细胞的增长并且在骨髓瘤房间导致房间 apoptosis 通过不同机制调停了,可能通过禁止 IL-6/STAT3 和 ERK1/2 小径。并且它可以为公里病人提供一种新治疗学的选择。 | Liangning Hu Huiqun Wu Bo Li Dongliang Song Guang Yang Gege Chen Bingqian Xie Zhijian Xu Yong Zhang Dandan Yu Jun Hou Wenqin Xiao Xi Sun Gaomei Chang Yiwen Zhang Lu Gao Bojie Dai Yi Tao Jumei Shi Weiliang Zhu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，5： | 5 |
| 4 | Targeting the PI3K/Akt/mTOR signaling pathway by pterostilbene attenuates mantle cell lymphoma progression显示文摘 | Dandan Yu Yong Zhang Gege Chen Yongsheng Xie Zhijian Xu Shuaikang Chang Liangning Hu Bo Li Wenxuan Bu Yingcong Wang Wenqin Xiao Xi Sun Gaomei Chang Lu Gao Sujing Qiang Xiaosong Wu Weiliang Zhu Jumei Shi | 2018 | Acta Biochimica et Biophysica Sinica2018，50，8： | 4 |
| 5 | DCZ0801,a novel compound,induces cell apoptosis and cell cycle arrest via MAPK pathway in multiple myeloma显示文摘Multiple myeloma(MM)is a refractory malignant hematological malignancy,and many therapeutic strategies have been developed to cure patients with MM.DCZ0801 is a compound that consists of oxophenamide and pterostilbene.The role of these compounds in hematological cancers such as MM has yet to be studied.In this study,we explored the potential mechanism of DCZ0801 action,its anti-tumor activity both in vitro and in vivo on MM.This study was carried out via cell cycle proliferation assay,apoptotic analysis,western blot analysis,and examination of xenotransplantation model of tumors.The in vitro studies revealed that DCZ0801 could inhibit cell proliferation and induce apoptosis by regulating both caspase-dependent and mitogen-activated protein kinase signaling pathways,inducing S-phase arrest of the cell cycle related to downregulation of CDK2,cyclin-A2,and CDC25A protein expression.The in vivo studies showed that DCZ0801 could significantly reduce the size of the tumors in nude mice.Our results demonstrated that DCZ0801 may emerge as the new therapeutic option for the patient with MM. | Ting Zhang Bo Li Qilin Feng Zhijian Xu Cheng Huang Huiqun Wu Zhangbo Chen Liangning Hu Lu Gao Peng Liu Guang Yang Hui Zhang Kang Lu Tingye Li Yi Tao Xiaosong Wu Jumei Shi Weiliang Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，5： | 3 |
| 6 | Myeloma cells resistance to NK cell lysis mainly involves an HLA class I-dependent mechanism显示文摘房间具有升起的生来的杀手(NK ) 的反多重的骨髓瘤(公里) 潜力对最近的年的兴趣。然而,到骨髓瘤房间的 NK 房间 cytotoxicity 的分子的机制仍然保持不清楚。在现在的学习,我们调查了人的白血球的 theexpressions 在耐心的骨髓瘤房间的抗原(HLA ) 一级和 HLA-G,并且决定了他们的关联住院病人肿瘤房间危险性到 NK 房间 cytotoxicity。我们的结果给那个病人看了骨髓瘤房间(n = 12 ) 对 NK-92 房间细胞溶解相对抵抗,与骨髓瘤房间线相比(n = 7, P < 0.01 ) 。基因表示介绍和流动 cytometry 分析证明 HLA 一级的 mRNA 和蛋白质是在 12 个耐心的骨髓瘤房间的 highlyexpressed。有趣地,没有或低 HLA-G 表面表示被检测,尽管多重 HLA-Gtranscripts 在这些骨髓瘤房间被检测。NK 房间功能试金证明由酸治疗的下面调整的 HLA 一级 expressionon 病人房间显著地增加了公里房间的危险性到调停 NK 的细胞溶解。而且,我们发现在骨髓瘤上用抗体堵住膜界限 HLA 一级而非 HLA-G 显著地取样 increasedtheir 危险性到调停 NK 的杀死。这些结果证明到 NK 的耐心的公里房间的抵抗 lysismainly 包含一个 HLA 班我依赖的机制,建议那 HLA 一级可以涉及保护公里房间调停 fromNK 在 vivo 攻击并且贡献他们的有免疫力的逃跑。 | Minjie Gao Lu Gao Guang Yang Yi Tao Jun Hou Hongwei Xu Xiaojing Hu Ying Han Qianqiao Zhang Xiaosong Wu Jumei Shi | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 3 |
| 7 | Proteasome inhibitor carfilzomib interacts synergistically with histone deacetylase inhibitor vorinostat in Jurkat T-leukemia cells显示文摘在现在的学习,我们在 Jurkat T 白血病房间调查了在 proteasome 禁止者 carfilzomib (CFZ ) 和 histone deacetylaseinhibitor vorinostat 之间的相互作用。到 CFZ 与的最低限度地致命的集中的细胞的 Coexposure 很, vorinostat 的 lowconcentration 导致了 synergistic antiproliferative 效果并且在 Jurkat T-leukemiacells 提高了 apoptosis ,与严厉地增加的反应的氧种( ROS )伴随了,在 mitochondrial membranepotential ( MMP )的惹人注目的减少,细胞色素 c 的增加的版本, caspase-9 和-3,和 PARP.The 的劈开的提高的激活联合了 Jurkat 细胞的处理与 ROS 预先对待而且, NAC 也在 apoptotic 房间导致了显著减小,显示为由联合处理的 increasedROS 产生的一个关键角色。另外,联合了逮捕的处理在 G 2-M 的房间周期阶段。这些结果暗示 CFZ 在 Jurkat T 白血病房间与 vorinostat synergistically 交往了,哪个有 vorinostat 的 carfilzomib 的联合可以在对待 T 房间代表新奇策略的 raisedthe 可能性白血病。 | Minjie Gao Lu Gao Yi Tao Jun Hou Guang Yang Xiaosong Wu Hongwei Xu Van S. Tompkins Ying Han Huiqun Wu Fenghuang Zhan Jumei Shi | 2014 | Acta Biochimica et Biophysica Sinica2014，46，6： | 3 |
| 8 | Tocilizumab in patients with moderate or severe COVID-19: a randomized, controlled, open-label, multicenter trial显示文摘Tocilizumab has been reported to attenuate the“cytokine storm”in COVID-19 patients.We attempted to verify the effectiveness and safety of tocilizumab therapy in COVID-19 and identify patients most likely to benefit from this treatment.We conducted a randomized,controlled,open-label multicenter trial among COVID-19 patients.The patients were randomly assigned in a 1:1 ratio to receive either tocilizumab in addition to standard care or standard care alone.The cure rate,changes of oxygen saturation and interference,and inflammation biomarkers were observed.Thirty-three patients were randomized to the tocilizumab group,and 32 patients to the control group.The cure rate in the tocilizumab group was higher than that in the control group,but the difference was not statistically significant(94.12%vs.87.10%,rate difference 95%CI−7.19%–21.23%,P=0.4133).The improvement in hypoxia for the tocilizumab group was higher from day 4 onward and statistically significant from day 12(P=0.0359).In moderate disease patients with bilateral pulmonary lesions,the hypoxia ameliorated earlier after tocilizumab treatment,and less patients(1/12,8.33%)needed an increase of inhaled oxygen concentration compared with the controls(4/6,66.67%;rate difference 95%CI−99.17%to−17.50%,P=0.0217).No severe adverse events occurred.More mild temporary adverse events were recorded in tocilizumab recipients(20/34,58.82%)than the controls(4/31,12.90%).Tocilizumab can improve hypoxia without unacceptable side effect profile and significant influences on the time virus load becomes negative.For patients with bilateral pulmonary lesions and elevated IL-6 levels,tocilizumab could be recommended to improve outcome. | Dongsheng Wang Binqing Fu Zhen Peng Dongliang Yang Mingfeng Han Min Li Yun Yang Tianjun Yang Liangye Sun Wei Li Wei Shi Xin Yao Yan Ma Fei Xu Xiaojing Wang Jun Chen Daqing Xia Yubei Sun Lin Dong Jumei Wang Xiaoyu Zhu Min Zhang Yonggang Zhou Aijun Pan Xiaowen Hu Xiaodong Mei Haiming Wei Xiaoling Xu | 2021 | Frontiers of Medicine2021，15，3： | 2 |
| 9 | PKC inhibition of sotrastaurin hasantitumor activity in diffuse large B-cell lymphoma via regulating the expression of MCT-1显示文摘MCT-1 (在 T 房间 lymphoma-1 的多重拷贝) ,新奇 oncogene,原来在 T 房间淋巴瘤被识别。最近的研究证明了 MCT-1 高度在 85% 弥漫的大 B 房间淋巴瘤(DLBCL ) 被表示。PKC (蛋白质 kinase C ) 为激活细胞的功能和增长为多重生物学上活跃的物质在信号 transduction 起一个必要作用。在这研究,我们发现 MCT-1 的 mRNA 和蛋白质表示层次显然在在 SUDHL-4 和 OCI-LY8 DLBCL 房间线由 siRNA 将 PKC 击倒以后被减少。在一个剂量依赖者和时间依赖者举止的一个选择 PKC 禁止者, sotrastaurin,有效地禁止的房间增长和导致的房间 apoptosis。同时,我们也观察到房间周期在对待 sotrastaurin 的房间在 G1 阶段被逮捕。另外, MCT-1 在在 vivo 的 sotrastaurin 处理组是下面调整的。而且,我们证明 PKC 禁止者 sotrastaurin 通过调整 MCT-1 的表示潜在地在 DLBCL 房间导致了房间 apoptosis 和房间周期拘捕。我们的数据建议那指向的 PKC 可以是为展出 MCT-1 的高水平的淋巴瘤和相关恶意的一条潜在的治疗学的途径蛋白质。 | Gaomei Chang Jiayi Zheng Wenqin Xiao Shuaikang Chang Qing Wei Huiqun Wu Yi Tao Guang Yang Bingqian Xie Xiucai Lan Yingcong Wang Dandan Yu Liangning Hu Yongsheng Xie Wenxuan Bu Yuanyuan Kong Bojie Dai Jun Hou Jumei Shi | 2018 | Acta Biochimica et Biophysica Sinica2018，50，4： | 2 |
| 10 | Quercetin inhibits the proliferation of multiple myeloma cells by upregulating PTPRR expression显示文摘Multiple myeloma (MM) is an incurable disease characterized by malignant plasma cell clonal expansion in the bone marrow;therefore, inhibiting the proliferation of plasma cells is an important approach to overcome the progression of MM. Quercetin (Que) is a promising flavonoid with broad-spectrum anti-tumor activity against various cancers, including MM;however, the underlying mechanism is not yet understood. The present study aimed to reveal the gene expression profile of Que-treated MM cells and clarify its potential mechanism. The 30% inhibitory concentration (IC30) of Que against MM cells was calculated, and the proliferation rate was significantly reduced after Que treatment. Next, 495 dysregulated genes were identified via RNA sequencing in Que-treated MM cells. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses indicated that the dysregulated genes were enriched in various apoptosis-related GO terms and amino acid metabolism-related pathways. qPCR validation showed that protein tyrosine phosphatase receptor-type R (PTPRR) had the highest verified log2 FC (abs) among the top 15 dysregulated genes. Overexpression of PTPRR increased the sensitivity of MM cells against Que, significantly inhibiting their proliferation and colony formation ability;silencing of PTPRR showed the opposite results. Furthermore, bioinformatics analyses and PPI network construction of PTPRR indicated that dephosphorylation of ERK might be the potential pathway for the PTPRR-induced inhibition of MM cell proliferation. In summary, our study identified the gene expression profile in Que-treated MM cells and demonstrated that the upregulation of PTPRR was one of the important mechanisms for the Que-induced inhibition of MM cell proliferation. | Houcai Wang Dandan Yu Hui Zhang Ruye Ma Huiqun Wu Huili Zhai Huaping Wang Jingjing Li Liping Li Yingcong Wang Taofang Cheng Jumei Shi | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 2 |
| 11 | Antitumor effect of dobutamine on multiple myeloma via mitogen-activated protein kinase pathway in vitro显示文摘多重骨髓瘤(公里) 是最恶意的癌症之一并且被房间, monoclonal 免疫球蛋白的分泌物和 osteolytic 损害,和它的发生在中年、老的病人正在增加的血浆被骨头髓的渗入描绘[1 ] 。作为公里房间异构并且药抵抗、起始的公里通常进行到 relapsed/refractory 阶段。尽管象镇静药, bortezomib,和 lenalidomide 那样的存在治疗能延长公里病人的幸存,公里是是仍然考虑了医不好。因此,建立新有效治疗对待 relapsed/refractory 公里是必要的。 | Bingqian Xie Zhijian Xu Guang Yang Gege Chen Bo Li Liangning Hu Wenqin Xiao Xi Sun Minjie Gao Lu Gao Xiaosong Wu Yi Tao Weiliang Zhu Jumei Shi | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 1 |
| 12 | DCZ3301, a novel aryl-guanidino inhibitor, induces cell apoptosis and cell cycle arrest via suppressing the PI3K/AKT pathway in T-cell leukemia/lymphoma显示文摘 | Wenqin Xiao Bo Li Xi Sun Dandan Yu Yongsheng Xie Huiqun Wu Shuaikang Chang Yunfei Zhou Houcai Wang Xiucai Lan Zhijian Xu Jumei Shi Weiliang Zhu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，7： | 1 |
| 13 | Anti-DLBCL efficacy of DCZ0825 in vitro and in vivo:involvement of the PI3K‒AKT‒mTOR/JNK pathway显示文摘Diffuse large B-cell lymphoma(DLBCL)is the most common type of non-Hodgkin lymphoma,characterized by high heterogeneity.The poor outcome of a portion of patients who suffer relapsing or resistant to conventional treatment impels the development of novel agents for DLBCL.DCZ0825 is a novel compound derived from pterostilbene and osalmide,whose antitumor activities have drawn our attention.In this study,we found that DCZ0825 exhibited high cytotoxicity toward DLBCL cell lines in a dose-and time-dependent manner,as revealed by cell counting kit-8 assay.Flow cytometry and western blot analysis results showed that DCZ0825 also promoted cell apoptosis via both extrinsic and intrinsic apoptosis pathways mediated by caspase.In addition,DCZ0825 induced cell cycle arrest in the G2/M phase by downregulating Cdc25C,CDK1,and Cyclin B1,thus interfering with cell proliferation.Further investigation showed the involvement of the phosphatidylinositol 3-kinase(PI3K)‒AKT‒mTOR/JNK pathway in the efficacy of DCZ0825 against DLBCL.Remarkably,DCZ0825 also exerted notable cytotoxic effects in vivo as well,with low toxicity to important internal organs such as the liver and kidney.Our results suggest that DCZ0825 may have the potential to become a novel anti-DLBCL agent or to replenish the conventional therapeutic scheme of DLBCL. | Ke Hu Bo Li Ruye Ma Hongfei Yi Zhijian Xu Yu Peng Dandan Yu Huiqun Wu Taofang Cheng Yumeng Lu Yong Zhang Rong Wei Guang Yang Xiaosong Wu Weiliang Zhu Jumei Shi | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 1 |
| 14 | Statins increase thrombomodulin expression and function in human endothelial cells by a nitric oxide-dependent mechanism and counteract tumor necrosis factor alpha-induced thrombomodulin downregulation显示文摘 | Jumei Shi Junru Wang Huaien Zheng Wen Ling Jacob Joseph Dayuan Li Jawahar L Mehta Usha Ponnappan Pei Lin Louis M Fink Martin Hauer-Jensen | 2003 | Blood Coagulation & Fibrinolysis2003，，6： | 1 |
| 15 | Galactic interstellar scintillation observed from four globular cluster pulsars by FAST显示文摘We report detections of scintillation arcs for pulsars in globular clusters M5,M13 and M15 for the first time using the Fivehundred-meter Aperture Spherical radio Telescope(FAST).From observations of these arcs at multiple epochs,we infer that screen-like scattering medium exists at distances 4.1_(-0.3)^(+0.2),6.7_(-0.2)^(+0.2)and 1.3_(-1.0)^(+0.7) kpc from Earth in the directions of M5,M13 and M15,respectively.This means M5's and M13's scattering screens are located at 3.0_(-0.2)^(+0.1) and 4.4_(-0.1)^(+0.1) kpc above the galactic plane,whereas,M15's is at 0.6_(-0.5)^(+0.3) kpc below the plane.We estimate the scintillation timescale and decorrelation bandwidth for each pulsar at each epoch using the one-dimensional auto-correlation in frequency and time of the dynamic spectra.We found that the boundary of the Local Bubble may have caused the scattering of M15,and detected the most distant off-plane scattering screens to date through pulsar scintillation,which provides evidence for understanding the medium circulation in the Milky Way. | Dandan Zhang Zhenzhao Tao Mao Yuan Jumei Yao Pei Wang Qijun Zhi Weiwei Zhu Xun Shi Michael Kramer Di Li Lei Zhang Guangxing Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，9： | 0 |
| 16 | Novel cyclophosphamide of natural products osalmide and pterostilbene induces cytotoxicity and cell cycle arrest in diffuse large B-cell lymphoma cells显示文摘Diffuse large B-cell lymphoma(DLBCL)is the most common category and disease entity of non-Hodgkin lymphoma.Osalmide and pterostilbene are natural products with anticancer activities via different mechanism.In this study,using a new synthetic strategy for the two natural products,we obtained the compound DCZ0801,which was previously found to have anti-multiple myeloma activity.We performed both in vitro and in vivo assays to investigate its bioactivity and explore its underlying mechanism against DLBCL cells.The results showed that DCZ0801 treatment gave rise to a dose-and time-dependent inhibition of cell viability as determined by CCK-8 assay and flow cytometry assay.Western blot analysis results showed that the expression of caspase-3,caspase-8,caspase-9 and Bax was increased,while BCL-2 and BCL-XL levels were decreased,which suggested that DCZ0801 inhibited cell proliferation and promoted intrinsic apoptosis.In addition,DCZ0801 induced G0/G1 phase arrest by downregulating the protein expression levels of CDK4,CDK6 and cyclin D1.Furthermore,DCZ0801 exerted an anti-tumor effect by down-regulating the expressions of p-PI3K and p-AKT.There also existed a trend that the expression of p-JNK and p-P38 was restrained.Intraperitoneal injection of DCZ0801 suppressed tum or development in xenograft mouse models.The preliminary metabolic study showed that DCZ0801 displayed a rapid metabolism within 30 min.These results demonstrated that DCZ0801 may be a new potential anti-DLBCL agent in DLBCL therapy. | Mengyu Xi Wan He Bo Li Jinfeng Zhou Zhijian Xu Huiqun Wu Yong Zhang Dongliang Song Liangning Hu Ye Lu Wenxuan Bu Yuanyuan Kong Gege Chen Shuaikang Chang Jumei Shi Weiliang Zhu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 0 |
| 17 | antitumor activity in bortezomib-resistant multiple myeloma cells through inhibition of JAK2/STAT3 pathway显示文摘Multiple myeloma(MM),the second most common haematological malignancy,is currently incurable because patients often develop multiple drug resistance and experience subsequent relapse of the disease.This study aims to identify a potential therapeutic agent that can counter bortezomib(BTZ)resistance in MM.DCZ0358,a novel alkaloid compound,is found to exert potent cytotoxic effects against BTZ-resistant MM cells in vivo and in vitro.The antimyeloma activity of DCZ0358 is associated with inhibition of cell proliferation,promotion of cell apoptosis via caspase-mediated apoptotic pathways,and induction of G0/G1 phase arrest via downregulation of cyclin D1,CDK4,and CDK6.Further investigation of the molecular mechanism shows that DCZ0358 suppresses the JAK2/STAT3 signaling pathway.In conclusion,DCZ0358 can successfully counter BTZ resistance in MM cells.This study provides evidence that warrants future preclinical assessments of DCZ0358 as a therapeutic agent against BTZ resistance in MM. | Bibo Zhang Bo Li Yongsheng Xie Shuaikang Chang Zhijian Xu Huifang Hu Gege Chen Ting Zhang Jun He Xiaosong Wu Huabin Zhu Weiming Lai Dongliang Song Ying Lu Xinyan Jia Weiliang Zhu Jumei Shi | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
| 18 | A novel silicone derivative of natural osalmid(DCZ0858)induces apoptosis and cell cycle arrest in diffuse large B-cell lymphoma via the JAK2/STAT3 pathway显示文摘Diffuse large B-cell lymphoma(DLBCL)is a highly heterogeneous malignant tumor characterized by diffuse growth.DCZ0858 is a novel small molecule with excellent antitumor effects in DLBCL.This study explored in depth the inhibitory effect of DCZ0858 on DLBCL cell lines.Cell Counting Kit-8(CCK-8)and plate colony formation assays were used to evaluate cell proliferation levels.Flow cytometry was employed to analyze apoptosis and the cell cycle,and western blotting was used to quantify the expression of cell cycle regulators.The results indicated that DCZ0858 inhibited cell growth in a concentration-dependent and time-dependent manner while inducing no significant toxicity in normal cells.Moreover,DCZ0858 initiated cell apoptosis via both internal and external apoptotic pathways.DCZ0858 also induced cell cycle arrest in the G0/G1 phase,thereby controlling cell proliferation.Further investigation of the molecular mechanism showed that the JAK2/STAT3 pathway was involved in the DCZ0858-mediated antitumor effects and that JAK2 was the key target for DCZ0858 treatment.Knockdown of JAK2 partly weakened the DCZ0858-mediated antitumor effect in DLBCL cells,while JAK2 overexpression strengthened the effect of DCZ0858 in DLBCL cells.Moreover,a similar antitumor effect was observed for DCZ0858 and the JAK2 inhibitor ruxolitinib,and combining the two could significantly enhance cancer-suppressive signaling.Tumor xenograft models showed that DCZ0858 inhibited tumor growth in vivo and had low toxicity in important organs,findings that were consistent with the in vitro data.In summary,DCZ0858 is a promising drug for the treatment of DLBCL. | Kang Lu Bo Li Hui Zhang Zhijian Xu Dongliang Song Lu Gao Haiguo Sun Liping Li Yingcong Wang Qilin Feng Gege Chen Liangning Hu Rong Wei Yongsheng Xie Dandan Yu Xiaosong Wu Weiliang Zhu Jumei Shi | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 19 | Dihydrocelastrol induces antitumor activity and enhances the sensitivity of bortezomib in resistant multiple myeloma by inhibiting STAT3-dependent PSMB5 regulation显示文摘Multiple myeloma(MM)is characterized by excessive aggregation of B-cell-derived malignant plasma cells in the hematopoietic system of bone marrow.Previously,we synthesized an innovative molecule named dihydrocelastrol(DHCE)from celastrol,a triterpene purified from medicinal plant Tripterygium wilfordii.Herein,we explore the therapeutic properties and latent signal transduction mechanism of DHCE action in bortezomib(BTZ)-resistant(BTZ-R)MM cells.In this study,we first report that DHCE shows antitumor activities in vitro and in vivo and exerts stronger inhibitory effects than celastrol on BTZ-R cells.We find that DHCE inhibits BTZ-R cell viability by promoting apoptosis via extrinsic and intrinsic pathways and suppresses BTZ-R MM cell proliferation by inducing G0/G1 phase cell cycle arrest.In addition,inactivation of JAK2/STAT3 and PI3K/Akt pathways are involved in the DHCE-mediated antitumor effect.Simultaneously,DHCE acts synergistically with BTZ on BTZ-R cells.PSMB5,a molecular target of BTZ,is overexpressed in BTZ-R MM cells compared with BTZ-S MM cells and is demonstrated to be a target of STAT3.Moreover,DHCE downregulates PSMB5 overexpression in BTZ-R MM cells,which illustrates that DHCE overcomes BTZ resistance through increasing the sensitivity of BTZ in resistant MM via inhibiting STAT3-dependent PSMB5 regulation.Overall,our findings imply that DHCE may become a potential therapeutic option that warrants clinical evaluation for BTZ-R MM. | Shuhan Jin Bo Li Bibo Zhang Xuejie Gao Xinyan Jia Li Xu Shuaikang Chang Ke Hu Guanli Wang Zhijian Xu Ting Zhang Dongliang Song Guang Yang Xiaosong Wu Huabin Zhu Cheng Huang Yumeng Lu Jumei Shi Weiliang Zhu Gege Chen | 2023 | Acta Biochimica et Biophysica Sinica2023，55，12： | 0 |
| 20 | Effect of Combined Foliar Spray on Heavy Metals Accumulation in Facility Fruit Vegetables显示文摘In order to explore the effects of combined foliar spray which was prepared from potassium fulvate and Zn on heavy metal accumulations in three kinds of facility fruit vegetables,cucumber,tomato and white melon,the foliar spray was applied from fruit setting to maturation.The results showed that the combined foliar spray significantly promoted the absorption of Zn and Cu in the three fruit vegetables,blocked the absorption of Cr,As and Pb in cucumber,significantly blocked the absorption of Cd in white melon,blocked the absorption of Pb in white melon,and showed a tendency of blocking the absorption of heavy metals Cd and As in tomato. | Huiwei ZHAO Jumei LI Xueping SHI Yiming LIU Chuan LU Shuo SUN | 2020 | Agricultural Biotechnology2020，9，5： | 0 |
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