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| 1 | Preparing graphene from anode graphite of spent lithium-ion batteries显示文摘 | Wenxuan Zhang Zhanpeng Liu Jing Xia Feng Li Wenzhi He Guangming Li Juwen Huang | 2017 | Frontiers of Environmental Science & Engineering2017，11，5： | 6 |
| 2 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 3 | Preparation of acellular nerve grafts with triton X-100显示文摘BACKGROUND: The source of nerve allograft enriches.We may choose expediently nerve allograft to repair injured nerve and the structure of choice nerve homlolgy or similar with the injured nerve,but the immunological rejiction limits the climical application of nerve allograft.The ideal substitute of autograft never is reaearching.OBJECTIVE: In this experiment,Triton X-100 was used to extract the Schwann cells and myelin sheaths of allograft nerve and obtain the inartiflcial and eliminated antigenicity nerve-transplanter(nerve grafts).DESIGN: Controlled experiment.SETTING: Department of Hand Surgery,the Third Affiliated Hospital of Hebei Medical University;Second Department of Orthopedics,Fourth Center Hospital of Tianjin.MATERIALS: Thirty heath New Zealand big ear white rabbit .of either sex(gender).weighing 2000-3000 g,were provided by the Center of Experimental Animal of Hebei Medical University.Triton X-100 was offered by SIGMA Company.METHODS: The experiment was carried out at the Central Laboratory of the Third Affiliated Hospital of Hebei Medical University from December 2003 to December 2004.Sixty pieces of sciatic nerves.10-mm-long nerve segment,which were taken from 30 rabbits,were incised.They were randomly divided into chemical extraction group(n=50)and control group(n=10).In the chemical extraction groups,the nerves were put into 3% Triton X-100 solution.They were treated with Triton X-100 for 12 hours,24 hours,48 hours,96 hours and 1 week.respectively.They were examined in every period.The control groups did not treated with anything. ①Respectively two segments of nerve by 2 mm length were taken from each nerve in the every periods.②The laminin immunohistochemical stained sections were performed with image acquisition and analyzed with multicolor pathological image analysis system.Measured the laminin antibody reaction part of each section and computed laminin average gray degrees of the unit area.All dates were analyzed by SPSS 10.0 software.MAIN OUTCOME MEASURES:①General observation and histological observation in two groups;②Compared with laminin average gray degrees of the unit area in each section.RESULTS:①General observation:In the control groups,fresh nerve was polish,rigidity and elasticity.After the nerves were chemical extracted,the floccules was seen at two ends and around of the nerves.The nerves being extraction presented ivory and lackluster. Its diameter and length compared reduced,tenderness and tenacity with the fresh nerve.0bserved by light microscope,Schwann cells,myelin sheaths and basement membrane distribute uniformly in control groups.After the nerves were extracted,Schwann cells and myelin sheaths disappeared. Basement membrane presented barrier array in longitudinal sections. Between the membranes was the basement membrane tube.Observed with scanning electron microscope,the basement membrane tubes composed by collagen fibers were remained and collagen fibers maintained their former position,form and structure.Further,the structure of membrane was seen in the tubes.It was Schwann cells basement membrane.②In chemical extraction groups,laminin average gray degrees of the unit area were 140.1±3.41(12 hours),142.1±3.14(24 hours),142.1±3.14(48 hours),140.4±4.03(96 hours),141.7±2.62(1 week).In the control groups,laminin average gray degree of the unit area was 142.7±7.24.There were not significant differences among the groups (P>0.05).CONCLUSION:The method of chemical extraction by using of Triton X-100 may be an ideal measure for preparing tissue-engineered nerve-transplanter and reserved the live of laminin in the basement membrane. | Jinbao Han1, Juwen Chen2, Baohui Zhao1, Jichun Zhang1, Dehu Tian1, Jiuhui Han1 1Department of Hand Surgery, the Third Affiliated Hospital, Hebei Medical University, Shijiazhuang 050051, Hebei Province, China 2Second Department of Orthopaedics, the Fourth Center Hospital of Tianjin, Tianjin 300140, China | 2006 | Neural Regeneration Research2006，1，7： | 2 |
| 4 | Substituent groups from aromatic dicarboxylates modulated structural diversification in the assembly of Co(Ⅱ)complexes based on the bis-pyridyl-bis-amide ligands显示文摘Four coordination polymers based on two bis-pyridyl-bis-amide ligands and three aromatic dicarboxylates with different substituent groups, namely, [Co(3-bpcb) 1.5 (NPH)(H2O)]·4H2O (1), [Co(3-bpcb)(NIPH)] (2), [Co(3-bpcb)0.5 (5-H2 AIP)]·2H2O (3), [Co(3-bpcd) 0.5 (5-H2 AIP)(H2O)]·2H2O (4) [3-bpcb = N,N′-bis(3-pyridinecarboxamide)-1,4-benzene, 3-bpcd = N,N′-bis(3- pyridine)cyclohexane-1,4-dicarboxamide, H2 NPH = 3-nitrophthalic acid, H2 NIPH = 5-nitroisophthalic acid, 5-H4 AIP = 5-aminoisophthalic acid] have been synthetized under hydrothermal conditions. Complex 1 displays a one dimensional (1D) chain based on the binuclear [Co2 (NPH)2 ] units and 3-bpcb ligands, which is extended into a three dimensional (3D) supramolecular framework through hydrogen bonding and - stacking interactions. In complex 2, the (3,5)-connected two dimensional (2D) layers are constructed from 1D Co-NIPH chains and bidentate-bridging 3-bpcb ligands. Complex 3 is a 2D double layer based on Co-5-H2 AIP 2D layers and 3-bpcb pillars. Complex 4 also displays a 2D network, which is constructed from the Co-5-H2AIP 1D double chains and 3-bpcd ligands. Finally, complexes 2-4 are extended into 3D supramolecular frameworks by hydrogen bonding or - stacking interactions. The substituent groups of dicarboxylates play an important role in the assembly and structures of the title complexes. In addition, the fluorescent properties of complexes 1-4 and the electrochemical behaviors of 3 and 4 at room temperature have been investigated. | WANG XiuLi MU Bao LIN HongYan YANG Song LIU GuoCheng TIAN AiXiang ZHANG JuWen | 2013 | Science China Chemistry2013，56，5： | 2 |
| 5 | Effects of Municipal Sewage Sludge on Fixation of Cr,Ni,Cu,and Zn during Co-processing of Heavy Metal-containing Waste in Cement Kilns显示文摘Cement raw meal with MSS and different heavy metals was blended to examine the fixation ratios, chemical species, and cement crystalline phases in clinkers. The results showed that blending MSS could decrease the fixation ratio of Cr, Ni, Cu, and Zn in the produced clinker by 5% to 25%. And Cr, Cu, and Zn were mainly incorporated into clinkers as metal silicates, Ni was mainly solubilized in Mg O to form magnesium nickel oxides, and the transition phases were mainly metal aluminum oxides as indicated by X-ray diffraction. The reduction of fixation ratios was likely attributed to the presence of impure elements, such as sodium and phosphorus in MSS. In addition, high concentrations(eg, 1.7 wt%) of chlorine in MSS led to metal chloride formation that could vaporize Cu, Cr, Ni, and Zn. To summarize, introducing MSS would decrease the fixation ratios of heavy metals due to the presence of impure elements, such as sodium and phosphorus and chlorine. | 刘伟 CAO Haihua 徐竟成 LIU Jia HUANG Juwen HUANG Xiangfeng LI Guangming | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，4： | 2 |
| 6 | Two directional seam tracking system with a scanning infrared sensor and its application in industry 显示文摘 | CHEN Wuzhu WANG Yong MA Juwen | 1994 | Welding in the World1994，34，7： | 1 |
| 7 | Predicting protein-protein interactions based only on sequences information显示文摘 | SHEN Juwen ZHANG Jian LuO Xiaomin ZHU Weiliang Yu Kunqian CHEN Kaixian LI Yixue JIANG Hualiang | 2007 | Proceedings of the National Academy of Sciences2007，104，11： | 1 |
| 8 | Cathode ray tube (CRT) recycling: Current capabilities in China and research progress显示文摘 | Qingbo Xu Guangming Li Wenzhi He Juwen Huang Xiang Shi | 2012 | Waste Management2012，，8： | 1 |
| 9 | Predicting protein-protein interaction based only on sequences information显示文摘 | Juwen Shen Hualiang Jiang | 2007 | Proceedings of the National Academy of Sciences2007，104，11： | 1 |
| 10 | Upgrading pyrolytic carbon-blacks (CBp) from end-of-life tires: Characteristics and modification methodologies显示文摘Over 1 billion end-of-life tires(ELTs)are generating annually,and 4 billion ELTs are currently abandoned in landfills and stockpiles worldwide,according to the statistics,leading to the environmental and health risks.To circumvent these issues,pyrolysis,as an attractive thermochemical process,has been addressed to tackle the ELTs'problem to reduce the risks as well as increase the material recycling.However,due to the lack of systematic characteristic analysis and modification methods,poor quality of CBp limits the improvement of ELTs pyrolysis in industry applications,which plays a crucial role in the economic feasibility of pyrolysis process.In this review,we have summarized the state-of-the-art characteristics and modification methodologies of the upgrading of CBp,to in-depth understand the surface microstructures and physiochemical properties o f CBp for the foundation for modification afterwards.By virtue of the proper selection of modification methods and modifying agents,the new generation of multifunctional carbon materials with desired properties can be instead of the traditional materials of CB,promising broader and various application fields. | Jiaxue Yu Junqing Xu Zhenchen Li Wenzhi He Juwen Huang Junshi Xu Guangming Li | 2020 | Frontiers of Environmental Science & Engineering2020，14，2： | 1 |
| 11 | Predicting protein- protein interactions based only on sequences information 显示文摘 | Shen Juwen Zhang Jian Luo Xiaomin | 2007 | Proc Natl Acad Sci2007，104，11： | 1 |
| 12 | Inhibition of thermal degradation of mackerel surimi by pig plasma protein and L- kininogen显示文摘 | LEE J aijaan TZENG S hinnshuenn WU Juwen | 2000 | Journal of Food Science2000，657，： | 1 |
| 13 | The microtubule inhibiting agent epothilone B antagonizes glioma cell motility associated with reorganization of the actin-binding protein α-actinin 4 显示文摘 | Whitney H Juwen D Quincy Q | 2011 | Oncology Reports2011，25，3： | 1 |
| 14 | Predicting protein-protein interactions based only on sequences information 显示文摘 | SHEN Juwen ZHANG Jian LUO Xiaomin | 2007 | Proc Natl Acad Sci USA2007，104，11： | 1 |
| 15 | Purification and characterization of a cystatin from the leaves of methyl jasmonate treated tomato plants显示文摘 | Juwen Wu Norman F Haard | 2000 | Comparative Biochemistry and Physiology Part C: Comparative Pharmacology and Toxicology2000，，2： | 1 |
| 16 | WEEE recovery strategies and the WEEE treatment status in China显示文摘 | Wenzhi He Guangming Li Xingfa Ma Hua Wang Juwen Huang Min Xu Chunjie Huang | 2006 | Journal of Hazardous Materials2006，，3： | 1 |
| 17 | ‘C-type’ closed state and gating mechanisms of K2P channels revealed by conformational changes of the TREK-1 channel显示文摘Two-pore domain potassium (K2P) channels gate primarily within the selectivity filter, termed ‘C-type’ gating. Due to the lack of structural insights into the nonconductive (closed) state, ‘C-type’ gating mechanisms remain elusive. Here, molecular dynamics (MD) simulations on TREK-1, a K2P channel, revealed that M4 helix movements induce filter closing in a novel ‘deeper-down’ structure that represents a ‘C-type’ closed state. The ‘down’ structure does not represent the closed state as previously proposed and instead acts as an intermediate state in gating. The study identified the allosteric ‘seesaw’ mechanism of M4 helix movements in modulating filter closing. Finally, guided by this recognition of K2P gating mechanisms, MD simulations revealed that gain-of-function mutations and small-molecule activators activate TREK-1 by perturbing state transitions from open to closed states. Together, we reveal a ‘C-type’ closed state and provide mechanical insights into gating procedures and allosteric regulations for K2P channels. | Qiansen Zhang Jie Fu Shaoying Zhang Peipei Guo Shijie Liu Juwen Shen Jiangtao Guo Huaiyu Yang | 2022 | Journal of Molecular Cell Biology2022，14，1： | 0 |
| 18 | Rethinking Art Education: Perception, Comprehension and Communication of Beauty Hsun Chiang: Beauty, The Invisible Competence, China CITIC Press, Beijing, 2011, 236 p., ￥35.00, ISBN: 9787508630236显示文摘 | Juwen Zhang | 2017 | Fudan Journal of the Humanities and Social Sciences2017，10，1： | 0 |
| 19 | 牛魔王:一位睿智的土财主显示文摘牛魔王的第一次出场就是在孙悟空从龙宫抢了金箍棒之后,那时候的花果山兵强马壮,颇有梁山108将齐聚时的声势。之后孙悟空邀游四海广交闲友,结拜了6个兄弟,其中老大就是牛魔王。然后孙悟空称“齐天大圣”,他也就称了所谓“平天大圣”。也许在那时候,年轻的牛魔王是想跟着孙悟空干一番大事业的, | juwen | 2010 | 各界2010，，1： | 0 |
| 20 | 《西游记》中最善良的妖怪显示文摘《西游记》中最能与人和谐相处、善良为妖的,应该是虎口洞的黄狮精。从书上的描述来看,它应该是在山里低调做妖的那种。当孙悟空问天竺王子附近是否有妖怪时,王子们说:“孤这州城之北,有一座豹头山。山中有一座虎口洞。往往人言洞内有仙,又言有虎狼,又言有妖怪。孤未曾访得端的,不知果是何物。”由此可见,它至少不像那种残害生灵兴风作浪的妖怪。 | Juwen | 2009 | 作文与考试(高中版)2009，，11： | 0 |
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