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| 1 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 2 | Abscisic acid dynamics, signaling, and functions in plants显示文摘Abscisic acid(ABA)is an important phytohormone regulating plant growth,development,and stress responses.It has an essential role in multiple physiological processes of plants,such as stomatal closure,cuticular wax accumulation,leaf senescence,bud dormancy,seed germination,osmotic regulation,and growth inhibition among many others.Abscisic acid controls downstream responses to abiotic and biotic environmental changes through both transcriptional and posttranscriptional mechanisms.During the past 20 years,ABA biosynthesis and many of its signaling pathways have been well characterized.Here we review the dynamics of ABA metabolic pools and signaling that affects many of its physiological functions. | Kong Chen Guo-Jun Li Ray ABressan Chun-Peng Song Jian-Kang Zhu Yang Zhao | 2020 | Journal of Integrative Plant Biology2020，62，1： | 46 |
| 3 | Relationship between DNA Barcoding and Chemical Classification of Salvia Medicinal Herbs显示文摘Objective To make the identification of medicinal herbs in Salvia L.quickly and accurately.Methods In this work,DNA barcoding and chemical fingerprint were compared for the identification of herbs in Salvia L.First,the nucleotide sequences of the internal transcribed spacer region two amplified from 48 medicinal plants in Salvia L.,and three other groups of medicinal plants in Lamiaceae were sequenced.A molecular phylogeny was constructed using the minimum evolution and maximum parsimony methods according to their sequence diversity.Second,the water-solution bioactive components and lipid soluble components were tested by HPLC.Then a chemical phylogeny was built using HPLC fingerprint data.Comparing the molecular and chemical phylogenetic trees revealed many similarities.Results DNA barcoding was sequencing based and could therefore provide more accurate results within a shorter time especially in large-scale studies.Conclusion The results show that ITS2 region is a novel DNA barcode for the authentication of the species in Salvia L.This is the first work to show the relationship between DNA barcoding and chemical components. | HAN Jian-ping1,LIU Chang2,LI Min-hui1,3,SHI Lin-chun1,SONG Jing-yuan1,YAO Hui1,PANG Xiao-hui1,CHEN Shi-lin1,4 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 2.Molecular Chinese Medicine Laboratory,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong,China 3.Baotou Medical College,Baotou 014040,China 4.Hubei University of Chinese Medicine,Wuhan 430065,China | 2010 | Chinese Herbal Medicines2010，2，1： | 23 |
| 4 | Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells显示文摘AIM:To investigate whether umbilical cord human mesenchymal stem cell(UC-MSC)was able to differentiate into neural stem cell and neuron in vitro.·METHODS:The umbilical cords were obtained from pregnant women with their written consent and the approval of the Clinic Ethnics Committee.UC-MSC were isolated by adherent culture in the medium contains 20%fetal bovine serum(FBS),then they were maintained in the medium contain 10%FBS and induced to neural cells in neural differentiation medium.We investigated whether UC-MSC was able to differentiate into neural stem cell and neuron in vitro by using flow cytometry,reverse transcriptase-polymerase chain reaction(RT-PCR)and immunofluorescence(IF)analyzes.·RESULTS:A substantial number of UC-MSC was harvested using the tissue explants adherent method at about 2wk.Flow cytometric study revealed that these cells expressed common markers of MSCs,such as CD105(SH2),CD73(SH3)and CD90.After induction of differentiation of neural stem cells,the cells began to form clusters;RT-PCR and IF showed that the neuron specific enolase(NSE)and neurogenic differentiation 1-positive cells reached 87.3%±14.7%and 72.6%±11.8%,respectively.Cells showed neuronal cell differentiation after induced,including neuron-like protrusions,plump cell body,obviously and stronger refraction.RT-PCR and IF analysis showed that microtubule-associated protein 2(MAP2)and nuclear factor-M-positive cells reached 43.1%±10.3%and 69.4%±19.5%,respectively.·CONCLUSION:Human umbilical cord derived MSCscan be cultured and proliferated in vitro and differentiate into neural stem cells,which may be a valuable source for cell therapy of neurodegenerative eye diseases. | Song Chen Wei Zhang Ji-Ming Wang Hong-Tao Duan Jia-Hui Kong Yue-Xin Wang Meng Dong Xue Bi Jian Song | 2016 | International Journal of Ophthalmology(English edition)2016，9，1： | 22 |
| 5 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 6 | Advances in the study of uncertainty quantification of large-scale hydrological modeling system显示文摘因为它不仅被物理因素而且由人的尺寸影响,地区性的水文学系统是极其复杂的。并且水文学模型在模仿复杂系统起一个很重要的作用。然而,由于它的复杂性和困难为模型可靠性和无常分析没有有效方法。在水文学建模的无常来自四个重要方面:在输入数据和参数的无常,在模型结构的无常,在分析方法的无常并且起始并且边界条件。这份报纸系统地在分析根据无常来源在大规模复杂水文学模型接近的无常的学习考察了最近的进展。另外,在为复杂水文学模型的无常分析的缺点和不足被指出。然后新无常 quantification 站台 PSUADE 和它的无常 quantification 方法被介绍,它将为大规模复杂水文学模型的无常分析是一个强大的工具和平台。最后,无常 quantification 上的一些未来观点被提出。 | SONG Xiaomeng ZHAN Chesheng KONG Fanzhe XIA Jun | 2011 | Journal of Geographical Sciences2011，21，5： | 21 |
| 7 | Interleukin-1 beta induction of neuron apoptosis depends on p38 mitogen-activated protein kinase activity after spinal cord injury^1显示文摘瞄准:Interleukin-1 贝它(IL-1beta ) 在脊髓损害(SCI ) 以后在神经原和 oligodendrocytes 在 apoptosis 的开始作为一个细胞外的信号被含有。为了进一步描绘 apoptotic 串联,在 SCI 以后由 IL-1beta 开始了,我们检验了 IL-1beta 的表示, p38 激活 mitogen 的蛋白质激酶(p38 MAPK ) 和在 SCI 以后的 caspase-3,并且进一步调查了 p38 MAPK 是否涉及 IL-1beta 导致的神经原 apoptosis。方法:老鼠与重量落下在 T-10 椎骨,脊椎水平被给殴打 SCI 的成年人影响或(10 g 重量扔了 25.0 公里) 。在 SCI 以后的 IL-1beta, p38 MAPK 和 caspase-3 的表示层次与西方的污点,染色的免疫组织化学,和实时反向的抄写聚合酶链反应(RT-PCR ) 被估计。神经原 apoptosis 与终端 deoxynucleotidyl 被估计标记的调停 transferase 的 deoxyuridine triphosphate-biotin 刻痕结束(TUNEL ) 方法。结果:IL-1beta 和 p38 MAPK 的增加的层次此后不久被观察损害,与在在损害的 6 h 以内的表示层次的一座山峰。由在损害以后的 24 h, caspase-3 表示显著地在受伤脊髓被增加。TUNEL 积极的房间首先在 lesioned 区域被观察在 SCI 以后的 6 h。TUNEL 积极的房间的最大的数字在 24 h post-SCI 被观察。IL-1 受体对手 IL-1Ra 的鞘内注射显著地减少了 p38 MAPK 和 caspase-3 的表示,并且减少了 TUNEL 积极的房间的数字。而且, p38 MAPK 的一个禁止者的鞘内注射, SB203580,显著地也减少了 caspase-3 的表示,并且在受伤脊髓减少了 TUNEL 积极的房间的数字。结论:表明小径的 p38MAPK 在 IL-1beta 起一个重要作用在老鼠的神经原 apoptosis 追随者 SCI 的调停的正式就职。 | Xin-jia WANG Kang-mei KONG Wei-li QI Wei-lian YE Pei-song SONG | 2005 | Acta Pharmacologica Sinica2005，26，8： | 19 |
| 8 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 9 | Radiofrequency ablation for hepatic hemangiomas: A consensus from a Chinese panel of experts显示文摘Recent studies have shown that radiofrequency(RF) ablation therapy is a safe, feasible, and effective procedure for hepatic hemangiomas, even huge hepatic hemangiomas. RF ablation has the following advantages in the treatment of hepatic hemangiomas: minimal invasiveness, definite efficacy, high safety, fast recovery, relatively simple operation, and wide applicability. It is necessary to formulate a widely accepted consensus among the experts in China who have extensive expertise and experience in the treatment of hepatic hemangiomas using RF ablation, which is important to standardize the application of RF ablation for the management of hepatic hemangiomas, regarding the selection of patients with suitable indications to receive RF ablation treatment, the technical details of the techniques, therapeutic effect evaluations, management of complications, etc. A final consensus by a Chinese panel of experts who have the expertise of using RF ablation to treat hepatic hemangiomas was reached by means of literature review, comprehensive discussion, and draft approval. | Jun Gao Rui-Fang Fan Jia-Yin Yang Yan Cui Jian-Song Ji Kuan-Sheng Ma Xiao-Long Li Long Zhang Chong-Liang Xu Xin-Liang Kong Shan Ke Xue-Mei Ding Shao-Hong Wang Meng-Meng Yang Jin-Jin Song Bo Zhai Chun-Ming Nin Shi-Gang Guo Zong-Hai Xin Jun Lu Yong-Hong Dong Hua-Qiang Zhu Wen-Bing Sun | 2017 | World Journal of Gastroenterology2017，23，39： | 15 |
| 10 | Study on the compatibility of cement-superplasticizer system based on the amount of free solution显示文摘The changes of free solution amount,fluidity and the time-depended fluidity loss of cement paste were examined by varying the water-cement ratio and the dosages of superplasticizer. The distribution of solution and flocculation microstructure in fresh cement paste was observed with optical microscope. The change of free solution amount and its effect on the fluidity and bleeding of cement paste was studied. The results show that the adsorbed solution amount has a great influence on the compatibility of cement-superplasticizer system,including the bleeding degree,the fluidity and the time-depended fluidity loss of cement paste. Superplasticizer increases the fluidity of cement paste by destroying the flocculated cement particle structure and increasing the amount of adsorbed solution. Polycarboxylate superplasticizer shows higher ability of adsorption than naphthalene superplasticizer. Over dosage of superplasticizer is not the primary cause of bleeding. The principle reason of bleeding is the high water-cement ratio and the insufficient enhancing ability of water adsorption of superplasticizer. | HAN Song YAN PeiYu KONG XiangMing | 2011 | Science China(Technological Sciences)2011，54，1： | 13 |
| 11 | 3D Shapeable,Superior Electrically Conductive Cellulose Nanofibers/Ti_(3)C_(2)T_(x) MXene Aerogels/Epoxy Nanocomposites for Promising EMI Shielding显示文摘In this work,3D highly electrically conductive cellulose nanofibers(CNF)/Ti_(3)C_(2)T_(x) MXene aerogels(CTA)with aligned porous structures are fabricated by directional freezing followed by freeze-drying technique,and the thermally annealed CTA(TCTA)/epoxy nanocomposites are then fabricated by thermal annealing of CTA,subsequent vacuum-assisted impregnation and curing method.Results show that TCTA/epoxy nanocomposites possess 3D highly conductive networks with ultralow percolation threshold of 0.20 vol%Ti_(3)C_(2)T_(x).When the volume fraction of Ti_(3)C_(2)T_(x) is 1.38 vol%,the electrical conductivity(σ),electromagnetic interference shielding effectiveness(EMI SE),and SE divided by thickness(SE/d)values of the TCTA/epoxy nanocomposites reach 1672 Sm-1,74 dB,and 37 dBmm-1,respectively,which are almost the highest values compared to those of polymer nanocomposites reported previously at the same filler content.In addition,compared to those of the samples without Ti_(3)C_(2)T_(x),the storage modulus and heat-resistance index of TCTA/epoxy nanocomposites are enhanced to 9792.5 MPa and 310.7℃,increased by 62%and 6.9℃,respectively,presenting outstanding mechanical properties and thermal stabilities.The fabricated lightweight,easy-to-process,and shapeable TCTA/epoxy nanocomposites with superior EMI SE values,excellent mechanical properties,and thermal stabilities greatly broaden the applications of MXene-based polymer composites in the field of EMI shielding. | Lei Wang Ping Song Cheng-Te Lin Jie Kong Junwei Gu | 2020 | Research2020，，1： | 13 |
| 12 | An efficient global sensitivity analysis approach for distributed hydrological model显示文摘水文学模型的敏感分析是为模型无常 quantification 的钥匙。然而,怎么有效地验证模型并且为分布式的水文学模型识别主导的参数是一个瓶颈完成参数优化。为这个原因,一条新途径在这份报纸,支持向量机器在被用来起初构造反应表面被建议。然后,它与 Sobol 方法集成基于 SVM 的反应表面,即 RSMSobol 方法,确定参数敏感。在这个工作,分布式的时间变体获得模型(DTVGM ) 被用于 Huaihe 河盆,它被用作一个盒子验证它的有效性和可行性。我们选择了三个客观函数(即水平衡系数 WB, Nash-Sutcliffe 效率系数 NS,和关联系数 RC ) 为敏感分析作为输出回答估计模型性能。参数 g1 和 g2 为所有目的是很重要的结果表演工作,并且他们是几乎对古典途径的一样。而且, RSMSobol 方法不能仅仅完成敏感的 quantification,并且也减少计算费用,与与古典途径相比的好精确性。并且这条途径将在为一个复杂建模系统的全球敏感分析有效、可靠。 | SONG Xiaomeng ZHAN Chesheng XIA Jun KONG Fanzhe | 2012 | Journal of Geographical Sciences2012，22，2： | 12 |
| 13 | Parameter identification and global sensitivity analysis of Xin'anjiang model using meta-modeling approach显示文摘Parameter identification, model calibration, and uncertainty quantification are important steps in the model-building process, and are necessary for obtaining credible results and valuable information. Sensitivity analysis of hydrological model is a key step in model uncertainty quantification, which can identify the dominant parameters, reduce the model calibration uncertainty, and enhance the model optimization efficiency. There are, however, some shortcomings in classical approaches, including the long duration of time and high computation cost required to quantitatively assess the sensitivity of a multiple-parameter hydrological model. For this reason, a two-step statistical evaluation framework using global techniques is presented. It is based on (1) a screening method (Morris) for qualitative ranking of parameters, and (2) a variance-based method integrated with a meta-model for quantitative sensitivity analysis, i.e., the Sobol method integrated with the response surface model (RSMSobol). First, the Morris screening method was used to qualitatively identify the parameters' sensitivity, and then ten parameters were selected to quantify the sensitivity indices. Subsequently, the RSMSobol method was used to quantify the sensitivity, i.e., the first-order and total sensitivity indices based on the response surface model (RSM) were calculated. The RSMSobol method can not only quantify the sensitivity, but also reduce the computational cost, with good accuracy compared to the classical approaches. This approach will be effective and reliable in the global sensitivity analysis of a complex large-scale distributed hydrological model. | Xiao-meng SONG Fan-zhe KONG Che-sheng ZHAN Ji-wei HAN Xin-hua ZHANG | 2013 | Water Science and Engineering2013，6，1： | 12 |
| 14 | Propofol and arrhythmias: two sides of the coin显示文摘催眠代理人 propofol 为麻醉的正式就职和维护是有效的。然而,最近的研究证明了 propofol 管理与心律不齐有关。Propofol 以一种集中依赖者方式显示支持 arrhythmic 和反没有节奏的效果。数据显示 propofol 能变换 supraventricular 心悸亢进和室的心悸亢进并且可以禁止心的传导系统。心脏的效果的机制仍然保持糟糕定义并且可以包含离子隧道,自治神经系统和心脏的差距连接。明确地,在心脏的房间的钠,钙和钾水流被 propofol 的临床上相关的集中压制。Propofol 弄短行动潜力持续时间(APD ) 但是在 APD 减少导致局部缺血的减少。而且, propofol 压制同情、副交感的音调并且在局部缺血期间保存差距连接。所有累积地这些效果贡献 propofol 的 antiarrhythmic 和 proarrhythmic 性质。 | Qiang LIU Ai-ling KONG Rong CHEN Cheng QIAN Shao-wen LIU Bao-gui SUN Le-xin WANG Long-sheng SONG Jiang HONG | 2011 | Acta Pharmacologica Sinica2011，32，6： | 10 |
| 15 | Promoter hypermethylation of death-associated protein kinase gene in cholangiocarcinoma显示文摘BACKGROUND:Death-associated protein kinase(DAPK) is a Ca2+/calmodulin-regulated Ser/Thr kinase which is involved in apoptosis. The aberrant methylation of its promoter region CpG islands may be one of the important mechanisms of carcinogenesis. We studied the relationship of methylation status and expression of the DAPK gene with the clinical findings in cholangiocarcinoma. METHODS:Target DNA was modified by sodium bisulfite,coverting all unmethylated,but not methylated,cytosines to uracil,and subsequently detected by methylation-specific PCR. Moreover,mRNA expression of the DAPK gene was assessed by RT-PCR. RESULTS:Aberrant methylation of the DAPK gene was detected in 11(30.6%) of 36 tissue specimens of cholangiocarcinoma,and in 2(5.6%) of 36 specimens of adjacent normal tissues. DAPK mRNA was not expressed in tumor and adjacent tissues with hypermethylation of the DAPK promoter. There were no statistical differences in the extent of differentiation and invasion,lymph node metastasis or pathologic type between the methylated and unmethylated tissues. CONCLUSIONS:The frequency of DAPK gene methylation in cholangiocarcinoma is high and it may offer an effective means for earlier auxiliary diagnosis of the malignancy. The DAPK gene is probably suppressed by methylation,and it could become resistant to apoptosis and immunologicalsurveillance. The DAPK gene epigenetically affected by methylation may be associated with the carcinogenesis of cholangiocarcinoma. | Liu, Xiao-Fang Kong, Fan-Min Xu, Zheng Yu, Shao-Ping Sun, Fu-Bo Zhang, Cui-Sheng Huang, Qing-Xian Zhou, Xian-Ting Song, Zhan-Wen | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，4： | 9 |
| 16 | Spatial-Temporal Changes in Grain Production, Consumption and Driving Mechanism in China显示文摘The spatial-temporal patterns of grain production and consumption have an important influence on the effective national grain supply on condition of tight balance in the total grain amount in China. In this paper, we analyze the spatial-temporal patterns of grain production, consumption and the driving mechanism for their evolution processes in China. The results indicate that both gravity centers of grain production and consumption in China moved toward the northern and eastern regions, almost in the same direction. The coordination of grain production and consumption increased slightly from 1995 to 2007 but decreased from 2000 to 2007. There is a spatial difference between the major districts of output increase and the strong growth potential in grain consumption, which indicates an increasing difficulty in improving the regional coordination of grain production and consumption. The movement of the gravity center of grain production is significantly correlated with regional differences in grain production policy, different economic development models, and spatial disparity of land and water resource use. For grain consumption, the main driving factors include rapid urbanization, the upgrade of food consumption structure, and distribution of food industries. | XU Shi-wei WU Jian-zhai SONG Wei LI Zhi-qiang LI Zhe-min KONG Fan-tao | 2013 | Journal of Integrative Agriculture2013，12，2： | 9 |
| 17 | A comparative study on the transplantation of different concentrations of human umbilical mesenchymal cells into diabetic rats显示文摘AIM: To observe the effects of intravitreal injections of different concentrations of human umbilical mesenchymal stem cells on retinopathy in rats with diabetes mellitus.METHODS: Healthy and adult male Sprague-Dawley(SD) rats were randomly assigned to a normal control group(group A), a diabetic retinopathy(DR) blank control group(group B), a high-concentration transplantation group(group C), a low-concentration transplantation group(group D) and a placebo transplantation group(group E). The expression of nerve growth factor(NGF)protein in the retinal layers was detected by immunohistochemical staining at 2, 4, 6 and 8wk.RESULTS: The expression of NGF was positive in group A and most positive in the retinal ganglion cell layer. In groups B and E, the expression of NGF was positive 2wk after transplantation and showed an increase in all layers. However, the level of expression had decreased in all layers at 4wk and was significantly reduced at 8wk. In groups C and D, the expression of NGF had increased at 2wk and continued to increase up to 8wk. The level of expression in group C was much higher than that in group D.CONCLUSION: DR can be improved by intravitreal injection of human umbilical mesenchymal stem cells.High concentrations of human umbilical mesenchymal stem cells confer a better protective effect on DR than low concentrations. | Jia-Hui Kong Dan Zheng Song Chen Hong-Tao Duan Yue-Xin Wang Meng Dong Jian Song | 2015 | International Journal of Ophthalmology(English edition)2015，8，2： | 9 |
| 18 | The synergistic effect of resveratrol in combination with cisplatin on apoptosis via modulating autophagy in A549 cells显示文摘几研究证明了有自然产品和化疗代理人的联合治疗能改进化疗代理人的 sensitivityand cytotoxicity。Resveratrol,一个自然产品,包括 antitumor andantiviral 活动,以及脉管的保护的效果有许多生物效果。这研究的目的是与 cisplatin 和涉及 A549 房间的潜在的 anticancer 机制在联合调查 resveratrol 的 synergistic anticancereffect。从数 Kit-8 和 isobolographic 分析的房间的 resultsobtained 表明了 resveratrol 和 cisplatin resultedin synergistic 的那联合在 A549 房间的细胞毒素的效果。从染色的 Hoechst 的结果,流动 cytometry 和西方的污点分析 suggestedthat resveratrol 提高了调停 cisplatin 的 apoptosis。同时, LC3-II 和 P62 层次和在有 cisplatin 的联合的 resveratrol 触发了的 autophagosomesuggested 的形成的变化 autophagy。更重要地,由 3-methyladeninemarkedly 禁止 autophagy 稀释了 resveratrol 和 cisplatin 的联合在 A549 房间引起的 apoptosis。一起拿,我们的 studyprovides resveratrol synergistically 与 cisplatin 相结合的第一条证据在 A549 房间经由 modulating autophagiccell 死亡导致 apoptosis。这些调查结果也帮助我们在肺癌症与 chemotherapyagents 在联合理解自然产品的角色。 | Song Hu Xiaolin Li Rongrong Xu Lingyun Ye Hui Kong Xiaoning Zeng Hong Wang Weiping Xie | 2016 | Acta Biochimica et Biophysica Sinica2016，48，6： | 9 |
| 19 | Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research. | LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER | 2008 | Chinese Science Bulletin2008，53，7： | 8 |
| 20 | Genome-wide identification and analysis of the DREB genes and their expression profiles under abiotic stresses in Chinese jujube(Ziziphus jujuba Mill.)显示文摘CBF/DREB proteins play a critical role in abiotic stress-mediated gene expression and represent attractive regulons for plant breeding programs.However,no study has been conducted for CBF/DREB protein-related genes in jujube(Ziziphus jujuba Mill.).In this study,twenty-five ZjDREB genes were identified and annotated from the jujube(Z.jujuba‘Dongzao’)genome.Detailed analysis,including gene classification,annotation,phylogenetic evaluation,conserved motif determination and expression profiling were performed on all genes.Phylogenetic analysis showed that ZjDREB proteins were divided into five subgroups(A1–A5),but lacking a subgroup A6 corresponding to AtDREBs.The ZjDREB genes were distributed in nine of twelve chromosomes in the genome.Additionally,the expression patterns of the DREB genes under different abiotic stresses were investigated using q RT-PCR.Nineteen ZjDREB genes were down-regulated under low temperature,in contrast six ZjDREB genes(01,03,05,11,23 and 24)were up-regulated.Under drought,salinity and high temperature conditions,expression of ZjDREB03,09,10,14,15,17 and 20 genes were induced and showed similar expression patterns,suggesting that various stress conditions share common elements in the signaling pathway.The results suggest that the family of DREB genes play an important role in abiotic stresses in jujube,and provide a foundation for further functional studies of this important class of transcriptional regulators. | Heying Zhou Jiping Jia Decang Kong Zhendong Zhang Shuang Song Yingyue Li Xiaoming Pang | 2019 | Journal of Forestry Research2019，30，4： | 8 |
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