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| 1 | Development of SNP markers using RNA-seq technology and tetraprimer ARMS-PCR in sweetpotato显示文摘The information of single nucleotide polymorphisms(SNPs) is quite unknown in sweetpotato. In this study,two sweetpotato varieties(Xushu 18 and Xu 781) were sequenced by Illumina technology,as well as de novo transcriptome assembly,functional annotation,and in silico discovery of potential SNP molecular markers. Tetra-primer Amplification Refractory Mutation System PCR(ARMS-PCR) is a simple and sufficient method for detecting different alleles in SNP locus. Total 153 sets of ARMS-PCR primers were designed to validate the putative SNPs from sequences. PCR products from 103 sets of primers were different between Xu 781 and Xushu 18 via agarose gel electrophoresis,and the detection rate was 67.32%. We obtained the expected results from 32 sets of primers between the two genotypes. Furthermore,we ascertained the optimal annealing temperature of 32 sets of primers. These SNPs might be used in genotyping,QTL mapping,or marker-assisted trait selection further in sweetpotato. To our knowledge,this work was the first study to develop SNP markers in sweetpotato by using tetra-primer ARMS-PCR technique. This method was a simple,rapid,and useful technique to develop SNP markers,and will provide a potential and preliminary application in discriminating cultivars in sweetpotato. | KOU Meng XU Jia-lei LI Qiang LIU Ya-ju WANG Xin TANG Wei YAN Hui ZHANG Yun-gang MA Dai-fu | 2017 | Journal of Integrative Agriculture2017，16，2： | 6 |
| 2 | Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes显示文摘Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication. | Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong | 2022 | Molecular Plant2022，15，2： | 5 |
| 3 | Synthesis, DNA-binding and photocleavage studies of ruthenium(Ⅱ) complexes [Ru(btz)_3]^(2+) and [Ru(btz)(dppz)_2]^(2+)显示文摘Two new ruthenium(Ⅱ) complexes, [Ru(btz)3](ClO4)2 (1) and [Ru(btz)(dppz)2](ClO4)2 (2) (btz = 4,4′-bithi-azole, dppz = dipyrido[3,2-a:2′,3′-c]phenazine), have been synthesized and characterized by elemental analysis, 1H NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via an intercalative mode. Under irradiation at 365 nm, both complexes were found to promote the cleavage of plasmid pBR 322 DNA from supercoiled form Ⅰ to nicked form Ⅱ. The mechanism studies reveal that singlet oxygen 1O2 and hydroxyl radical (OH-) play a significant role in the photocleavage process. | YU HuiJuan, HUANG ShuMei, KOU JunFeng, LI LvYing, JIA HaiNa, CHAO Hui & JI LiangNian MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China | 2009 | Science China Chemistry2009，52，9： | 4 |
| 4 | Ipomoea batatas HKT1 transporter homolog mediates K^+ and Na^+ uptake in Saccharomyces cerevisiae显示文摘Soil salinity causes the negative effects on the growth and yield of crops. In this study,two sweet potato(Ipomoea batatas L.) cultivars,Xushu 28(X-28) and Okinawa 100(O-100),were examined under 50 and 100 mmol L^(–1) Na Cl stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly,real-time quantitative polymerase chain reaction(RT-q PCR) results indicated that sweet potato high-affinity K^+ transporter 1(IbHKT1) gene expression was highly induced by 50 and 100 mmol L^(–1) Na Cl stress in the stems of X-28 cultivar than in those of O-100 cultivar,but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter,we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium(Na^+). Yeast complementation assay showed that IbHKT1 could take up K^+ in 50 mmol L^(–1) K^+ medium without the presence of Na Cl. Moreover,Na^+ uptake significantly increased in yeast overexpressing IbHKT1. These results showed that IbHKT1 transporter could have K^+-Na^+ symport function in yeast. Therefore,the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other HKT1 transporters in class I. Potentially,IbHKT1 could be used to improve the salt tolerance nature in sweet potato. | PARK Sung-chul YU Yi-cheng KOU Meng YAN Hui TANG Wei WANG Xin LIU Ya-ju ZHANG Yun-gang KWAK Sang-soo MA Dai-fu SUN Jian LI Qiang | 2017 | Journal of Integrative Agriculture2017，16，10： | 3 |
| 5 | Amyloid precursor protein and growth-associated protein 43 expression in brain white matter and spinal cord tissues in a rat model of experimental autoimmune encephalomyelitis显示文摘Studies have demonstrated that amyloid precursor protein(APP)expression increases in multiple sclerosis tissues during acutely and chronically active stages.To determine the relationship between axonal injury and regeneration in multiple sclerosis,an animal model of experimental autoimmune encephalomyelitis was induced using different doses of myelin basic protein peptide.APP and growth-associated protein 43(GAP-43),which is considered a specific marker of neural regeneration,were assessed by western blot analysis.Expression of APP and GAP-43,as well as the correlation between these two proteins,in brain white matter and spinal cord tissues of experimental autoimmune encephalomyelitis rats at different pathological stages was analyzed.Results showed that APP and GAP-43 expression increased during the acute stage and decreased during remission,with a positive correlation between APP and GAP-43 expression in brain white matter and spinal cord tissues.These results suggest that APP and GAP-43 could provide nutritional and protective effects on damaged neurons. | Yizhou Wang Shuang Kou Jingcheng Tang Ping Zhang Qiuxia Zhang Yan Liu Qi Zheng Hui Zhao Lei Wang | 2011 | Neural Regeneration Research2011，6，2： | 3 |
| 6 | Transarterial chemoembolization with hepatic arterial infusion chemotherapy plus S-1 for hepatocellular carcinoma显示文摘BACKGROUND Transarterial chemoembolization(TACE)and hepatic arterial infusion chemotherapy(HAIC)have shown promising local benefits for advanced hepatocellular carcinoma(HCC).S-1,a composite preparation of a 5-fluorouracil prodrug,has proven to be a convenient oral chemotherapeutic agent with definite efficacy against advanced HCC.AIM To evaluate the efficacy and safety of TACE followed by HAIC with or without oral S-1 for treating advanced HCC.METHODS In this single-center,open-label,prospective,randomized controlled trial,117 participants with advanced HCC were randomized to receive TACE followed by oxaliplatin-based HAIC either with(TACE/HAIC+S-1,n=56)or without(TACE/HAIC,n=61)oral S-1 between December 2013 and September 2017.Two participants were excluded from final analysis for withdrawing consent.The primary endpoint was progression-free survival(PFS)and secondary endpoints included overall survival(OS),objective response rate,disease control rate and safety.RESULTS In total,115 participants(100 males and 15 females;mean age,57.7 years±11.9)were analyzed.The median PFS and OS were 5.0 mo(0.4–58.6 mo)(95%confidence interval(CI):3.82 to 6.18)vs 4.4 mo(1.1–54.4 mo)(95%CI:2.54 to 6.26;P=0.585)and 8.4 mo(0.4–58.6 mo)(95%CI:6.88 to 9.92)vs 8.3 mo(1.4–54.4 m)(95%CI:5.71 to 10.96;P=0.985)in the TACE/HAIC+S-1 and TACE/HAIC groups,respectively.The objective response rate and disease control rate were 30.9%vs 18.4%and 72.7%vs 56.7%in the TACE/HAIC+S-1 and TACE/HAIC groups,respectively.Grade 3/4 adverse events had a similar frequency in both treatment groups.CONCLUSION No improvements in tumor response rates,PFS or OS were observed with the addition of S-1 to TACE/HAIC in advanced HCC.Both treatment regimens had a similar safety profile. | Jian-Hai Guo Shao-Xing Liu Song Gao Fu-Xin Kou Xin Zhang Di Wu Xiao-Ting Li Hui Chen Xiao-Dong Wang Peng Liu Peng-Jun Zhang Hai-Feng Xu Guang Cao Lin-Zhong Zhu Ren-Jie Yang Xu Zhu | 2020 | World Journal of Gastroenterology2020，26，27： | 3 |
| 7 | Absorption and Capture of Methane into Ionic Liquid显示文摘一个可逆存储版本过程由 10 的温差切换 ?? 在室温附近的 6 号元素碳的化学符号能被认识到。这快, recyclable,精力在大量温度和压力以内的有效、低费用和绿系统第一次这里被报导。系统被相信为存储和甲烷的同类的利用开创一条新线路。给词调音:离子的液体;甲烷;吸收;俘获;极性; | Yuan Kou Wei Xiong Guohong Tao Hui Liu Tao Wang | 2006 | Journal of Natural Gas Chemistry2006，15，4： | 3 |
| 8 | Synthesis of pillar[7]arene显示文摘支柱的第一合成[7 ] arene 与二个方法被报导。方法 A:有在 CHCl3 的 paraformaldehyde 的 1,4-dimethoxybenzene (1 ) 的催化 FeCl3 的冷凝作用反应给了 dimethoxypillar [7 ] arene (3 ) 。方法 B:p-toluenesulfonic 酸催化在 CH2Cl2 的 -1,4-dimethoxybenzene (2 ) 给了化合物 3 的 2,5 二度(benzyloxymethyl ) 的冷凝作用反应。有 BBr3 的 3 的 Demethylation 给了支柱[7 ] arene (4 ) 。支柱[7 ] arene 可能是在 hostguest 化学的一位观点 macrocyclic 主人。 | Yu Chen Hong Qi Tao Yu Hui Kou Herbert Meier Jian Long Fu De Rong Cao | 2012 | Chinese Chemical Letters2012，23，5： | 3 |
| 9 | Finite element simulation on the deep drawing of titanium thin-walled surface part显示文摘The deep drawing of titanium thin-walled surface part was simulated based on a self-developed three-dimensional finite element model. After an investigation on forming rules, a virtual orthogonal experimental design was adopted to determine the significance of processing parameters, such as die radius, blank holder force, and friction coefficient, on the forming process. The distributions of thickness and equivalent plastic strain of the drawn part were evaluated. The results show that die radius has a relative major influence on the deep drawing process, followed by friction coefficient and blank holder force. | GAO Enzhi, LI Hongwei, KOU Hongchao, CHANG Hui, LI Jinshan, and ZHOU Lian State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi’an 710072, China | 2010 | Rare Metals2010，29，1： | 2 |
| 10 | Resection of a retrohepatic leiomyosarcoma of the inferior vena cava combined with caudate lobectomy and reconstruction with an allogenic vein显示文摘Primary leiomyosarcoma of the inferior vena cava(IVC)is a rare disease,accounting for 0.5%of soft tissue sarcomas in adults[1].A diversity of therapeutic methods have been applied to treat this type of tumor.The average survival time of patients who are not treated is merely 3-4 months[2].The effect of radiotherapy and chemotherapy remains unclear and controversial[3].On the other hand,the en-bloc resection with negative margins may be the only potentially curative treatment and therefore,can contribute to the long-term survival of the patients[4].During the operation,a reconstruction graft for the defected IVC needs to be chosen[5]as the graft-related complications are the surgeons’major concern,which include graft thrombosis and infection[6].Growing evidence has suggested that an allogeneic vein from donation after brain death or cardiac death is a better choice to avoid these complications[7].An allogeneic vein has been reported to be safely used in patients with pancreatic cancer in case of the portal vein and/or superior mesenteric vein invasion[8].A cryopreserved allograft can even be applied in the management of native and prosthetic aortic infections[7].Herein,we present a case of resection of a retrohepatic leiomyosarcoma of the IVC combined with partial caudate lobectomy and reconstruction with an allogeneic vein. | Xin-Xue Zhang Ji-Qiao Zhu Hui Zhang Jian-Tao Kou Jun Ma Qiang He | 2021 | Hepatobiliary & Pancreatic Diseases International2021，20，1： | 2 |
| 11 | Nano-polycrystalline diamond formation under ultra-high pressure显示文摘 | Chao Xu Duanwei He Haikuo Wang Junwei Guan Chunmei Liu Fang Peng Wendan Wang Zili Kou Kai He Xiaozhi Yan Yan Bi Lei Liu Fengjiao Li Bo Hui | 2013 | International Journal of Refractory Metals and Hard Materials2013，，： | 2 |
| 12 | Association between interleukin-21 gene rs907715 polymorphism and gastric precancerous lesions in a Chinese population显示文摘BACKGROUND The single nucleotide polymorphisms of interleukin-21(IL-21)gene were confirmed to be related to various diseases,but no studies have examined the possible role of IL-21 single nucleotide polymorphisms(SNPs)(rs907715,rs2221903,and rs12508721)in gastric precancerous lesions.AIM To explore the associations between SNPs of IL-21 gene(rs907715,rs2221903,and rs12508721)and gastric precancerous lesions in a Chinese population.METHODS Three SNPs of IL-21 were genotyped using polymerase chain reaction–ligase detection reaction in 588 cases and 290 healthy controls from May 2013 to December 2016 in northwestern China.Gastric precancerous lesions were confirmed by endoscopic examination and categorized as non-atrophic gastritis,atrophic gastritis,and intestinal metaplasia.Descriptive statistic and logistic regression were used for data analyses.RESULTS IL-21 rs907715 genotype CC and C frequencies were higher in in patients with gastric precancerous lesions than in the controls(OR=1.59,95%CI:1.06-2.38,P=0.013;OR=1.28,95%CI:1.01-2.22,P=0.044,respectively)after adjusting for confounding factors.For SNP rs907715 in intestinal metaplasia patients,significant differences between cases and controls were observed in the frequencies of genotype CC and C(OR=1.92,95%CI:1.24-2.98,P=0.004;OR=1.53,95%CI:1.04-2.24,P=0.028,respectively);for non-atrophic gastritis and atrophic gastritis patients,the CC and C genotypes showed no significant association with risk in all models.No association between either rs2221903 or rs12508721 and gastric precancerous lesions was found in the present study.In the haplotype analysis,the TC haplotype(rs907715 and rs12508721)and TT haplotype(rs2221903 and rs907715)were more frequent in the case group than control group(P<0.05).CONCLUSION Our findings indicate that SNP rs907715 of IL-21 gene is associated with gastric precancerous lesions.The TC haplotype(rs907715 and rs12508721)and TT haplotype(rs2221903 and rs907715)increased the risk of gastric precancerous lesions.If confirmed,these findings will shed light on the etiology of precancerous lesions. | Xiao-Qin Wang Yang Li Paul D Terry Wen-Jing Kou Yue Zhang Zhao-Zhao Hui Xiao-Han Ren Ming-Xu Wang | 2020 | World Journal of Gastrointestinal Oncology2020，12，3： | 2 |
| 13 | Strongly Zero-Dimensional Locales | KOU Hui LUO Mao Kang Department of Mathematics. Sichuan University, Chengdu 610064. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，1： | 1 |
| 14 | Shikonin derivatives inhibited LPS-induced NOS in RAW 264.7 cells via downregulation of MAPK/NF-κB signaling显示文摘 | Yu Wen Cheng Ching Yi Chang Kou Lung Lin Chien Ming Hu Cheng Hui Lin Jaw Jou Kang | 2008 | Journal of Ethnopharmacology2008，，2： | 1 |
| 15 | Non-isothermal phase transformation kinetics of ω phase in TB-13 titaniumalloys显示文摘 | ZHOU Zhong-bo LAI Ming-jie TANG Bin KOU Hong-chao CHANG Hui ZHU Zhi-shou LI Jin-shan ZHOU Lian | 2010 | Materials Science and Engineering A2010，527，： | 1 |
| 16 | In- fluence of solution temperature on phase transformation of TC21 alloy显示文摘 | WANG YIHONG KOU HONGCHAO CHANG HUI | | 0，，： | 1 |
| 17 | Synthesis,crystal structures,electrochemical and spectroscopic properties of ruthenium(Ⅱ) complexes containing diamino-1,3,5-triazine derivatives显示文摘Three Ru(Ⅱ) complexes [Ru(bpy)2(1-IQTNH)](ClO4)2 (1), [Ru(bpy)2(2-QTNH)](ClO4)2 (2) and [Ru(bpy)2(3-IQTNH)](ClO4)2 (3) (bpy = 2,2′-bipyridine, 1-IQTNH = 6-(isoquinolin-1-yl)-1,3,5-triazine-2,4-diamine, 2-QTNH = 6-(quinolin-2-yl)-1,3,5-triazine-2,4-diamine, 3-IQTNH = 6-(isoquinolin-3-yl)-1,3,5-triazine-2,4-diamine) have been synthesized and characterized by elemental analysis, 1H NMR spectroscopy, electrospray ionization mass spectrometry and X-ray crystallography. The electrochemical and spectroscopic properties of the complexes differ from those of [Ru(bpy)3]2+ owing to the structural differences between the ligands and their complexes. | CHEN Yu, XU WenChao, KOU JunFeng, WEI XuHui, YU BoLe, CHAO Hui & JI LiangNian MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China | 2010 | Science China Chemistry2010，53，10： | 1 |
| 18 | The reflective and coreflective subcatego- ties of the complete semilattice category显示文摘 | Kou Hui | 1998 | Jour- nal of Sichuan University: Natural Science Edtion1998，35，4： | 1 |
| 19 | Identification of Inonotus obliquus and Analysis of Antioxidation and Antitumor Activities of Polysaccharides显示文摘 | Yana Song Jing Hui Wei Kou Ru Xin Fei Jia Ning Wang Fengqing Hu Huili Zhang Hongsheng Liu | 2008 | Current Microbiology2008，，5： | 1 |
| 20 | Genetic lineage tracing identifies cardiac mesenchymal-to-adipose transition in an arrhythmogenic cardiomyopathy model显示文摘Arrhythmogenic cardiomyopathy(ACM)is one of the most common inherited cardiomyopathies,characterized by progressive fibrofatty replacement in the myocardium.However,the cellular origin of cardiac adipocytes in ACM remains largely unknown.Unraveling the cellular source of cardiac adipocytes in ACM would elucidate the underlying pathological process and provide a potential target for therapy.Herein,we generated an ACM mouse model by inactivating desmosomal gene desmoplakin in cardiomyocytes;and examined the adipogenic fates of several cell types in the disease model.The results showed that SOX9^(+),PDGFRa^(+),and PDGFRb^(+)mesenchymal cells,but not cardiomyocytes or smooth muscle cells,contribute to the intramyocardial adipocytes in the ACM model.Mechanistically,Bmp4 was highly expressed in the ACM mouse heart and functionally promoted cardiac mesenchymal-to-adipose transition in vitro. | Xinyan Huang Lei Yan Jufeng Meng Nanbo Liu Shuoji Zhu Zhen Jiang Shan Kou Teng Feng Chao-Po Lin Bin Zhou Juan Tang Ping Zhu Hui Zhang | 2023 | Science China(Life Sciences)2023，66，1： | 1 |
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