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| 1 | Qingnaoyizhi decoction suppresses the formation of glial fibrillary acidic protein-positive cells in cultured neural stem cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway显示文摘OBJECTIVE: Inactivation of the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling axis plays a crucial role in determining the fate of neural stem cells(NSCs).Qingnaoyizhi decoction(QNYZD) has been used for the treatment of vascular dementia and has shown to improve synaptic remodeling. The aim of this study was to evaluate the effect of cerebrospinal fluid(CSF) containing QNYZD(CSF-QNYZD) on the differentiation of cultured NSCs and the involvement of the JAK2/STAT3 pathway.METHODS: The protein expression levels of glial fibrillary acidic protein(GFAP), tubulin, drosophila mothers against decapentaplegic protein(SMAD-1), STAT3, and phosphorylated-STAT3 were detected by western immunoblot analysis in the groups: control, CSF, JAK/STAT inhibitor(AG490),CSF-QNYZD, and CSF-XDZ(CSF-Xidezhen). The differentiation of NSCs was determined by immunofluorescence staining. The proliferation of NSCs was measured using the Cell Counting Kit-8 proliferation assay.RESULTS: Compared with the control group,CSF-QNYZD and AG490 significantly increased the number and expression of tubulin-positive cells, reduced the number and expression of GFAP-positive cells, and down-regulated the expression of p-STAT3. However, CSF-QNYZD also decreased the expression of SMAD-1 and STAT3.CONCLUSION: Enhanced neuronal differentiation may be associated with the down-regulation of glial differentiation instead of promoting proliferationin treated NSCs. Furthermore, QNYZD may play a direct role in suppressing the formation of GFAP-positive cells and enhancing neuronal differentiation by inhibiting JAK2/STAT3 activation. Overall, these results provide insights into the possible mechanism underlying QNYZD-mediated neurogenesis. | Wu Yanqing Jing Zhiwei Qin Xiude Zhou Zhen Wang Kai Song Wanshan Wang Xueyan Hou Mengmeng Zhang Yulian Kang Liyuan | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 11 |
| 2 | A meta-analysis of Chinese herbal medicines for vascular dementia显示文摘OBJECTIVE: To investigate the efficacy and safety of Chinese herbal medicines in the treatment of patients with vascular dementia. DATA RETRIEVAL: We retrieved publications from Cochrane Library (2004 to July 2011), PubMed (1966 to July 2011), the Chinese Science and Technique Journals Database (1977 to July 2011), the China National Knowledge Infrastructure (1979 to July 2011), Google Scholar (July 2011), and the Chinese Biomedical Database (1977 to July 2011) using the'Chinese medicine OR Chinese herbal medicine' and 'vascular dementia OR mild cognition impair OR multi-infarct dementia OR small-vessel dementia OR strategic infarct dementia OR hypoperfusion dementia OR hemorrhagic dementia OR hereditary vascular dementia'. SELECTION CRITERIA: Randomized controlled trials comparing Chinese herbal medicines with placebo/western medicine in the treatment of patients with vascular dementia were included. Diagnostic standards included Diagnostic and Statistical Manual of Mental Disorders-IV, and National Institute of Neurological Disorders and Stroke and Association Internationale pour la Recherché et l'Enseignement en Neurosciences. Two participants independently conducted literature screening, quality evaluation and data extraction. The quality of each trial was assessed according to the Cochrane Reviewers' Handbook 5.0. MAIN OUTCOME MEASURES: Effective rate, Mini-Mental State Examination scores, Hasegawa Dementia Scale scores, and incidence of adverse reactions. RESULTS: We identified 1 143 articles discussing the effects of Chinese medicine on vascular dementia. Thirty-one of these were included in the analysis. These studies involved a total of 2 868 participants (1 605 patients took Chinese medicine decoctions (treatment group); 1 263 patients took western medicine or placebo). The results of our meta-analysis revealed that Chinese herbal remedies in the treatment group were more efficacious than the control intervention (relative risk (RR)=1.27; 95% confidence interval (CI): 1.18-1.38, P<0.01). Mini-Mental State Examination scores were higher in patients taking Chinese herbal medicines than in those in the control group (weighted mean difference (WMD)=2.83; 95%CI: 2.55-3.12, P<0.01). Patients in the treatment group showed better disease amelioration than those in the control group (Hasegawa Dementia Scale scores; WMD=2.41, 95%CI: 1.48-3.34, P<0.01). There were also considerably fewer adverse reactions among those in the treatment group compared with those in the control group (RR=0.20, 95%CI: 0.08-0.47, P<0.01). CONCLUSION: Chinese herbal medicine appears to be safer and more effective than control measures in the treatment of vascular dementia. However, the included trials were generally low in quality. More well-designed, high-quality trials are needed to provide better evidence for the assessment of the efficacy and safety of Chinese medicines for vascular dementia. | Xiude Qin Yu Liu Yanqing Wu Shuo Wang Dandan Wang Jinqiang Zhu Qiaofeng Ye Wei Mou Liyuan Kang | 2013 | Neural Regeneration Research2013，8，18： | 7 |
| 3 | Efficient and stable tin-based perovskite solar cells by introducing π-conjugated Lewis base显示文摘Tin-based perovskite solar cells(TPSCs)as the most promising candidate for lead-free PSCs have incurred extensive researches all over the world.However,the crystallization process of tin-based perovskite is too fast during the solution-deposited process,resulting in abundant pinholes and poor homogeneity that cause serious charge recombination in perovskite layer.Here,we employed theπ-conjugated Lewis base molecules with high electron density to systematically control the crystallization rate of FASnI3 perovskite by forming stable intermediate phase with the Sn-I frameworks,leading to a compact and uniform perovskite film with large increase in the carrier lifetime.Meanwhile,the introduction of theπ-conjugated systems also retards the permeation of moisture into perovskite crystal,which significantly suppresses the film degradation in air.These benefits contributed to a stabilizing power conversion efficiency(PCE)of 10.1%for the TPSCs and maintained over 90%of its initial PCE after 1000-h light soaking in air.Also,a steady-state efficiency of 9.2%was certified at the accredited test center. | Tianhao Wu Xiao Liu Xin He Yanbo Wang Xiangyue Meng Takeshi Noda Xudong Yang Liyuan Han | 2020 | Science China Chemistry2020，63，1： | 6 |
| 4 | High-speed visual target tracking with mixed rotation invariant description and skipping searching显示文摘This paper proposes a novel high-speed visual target tracking system based on mixed rotation invariant description(MRID) and skipping searching method. MRID is a novel rotation invariant description of texture and edge information by annular histograms and dominant direction. It overcomes rotation variant and large computation issues in conventional LBP-HOG feature description. The skipping searching method used in tracking can remarkably decrease the computation time by avoiding repeated searching operations.The proposed tracking system contains an image sensor, a hierarchical vision processor and an actuator with2 dimensions of freedom(DOF). The vision processor integrates processors with pixel-and row-level parallelism to speed up the tracking algorithm. Experiment results show that the proposed system can achieve over 1000-fps processing speed of the tracking algorithm under 750 × 480 resolution image. | Yongxing YANG Jie YANG Zhongxing ZHANG Liyuan LIU Nanjian WU | 2017 | Science China(Information Sciences)2017，60，6： | 5 |
| 5 | Strength and deformation behaviors of veined marble specimens after vacuum heat treatment under conventional triaxial compression显示文摘The mechanical behaviors of rocks affected by high temperature and stress are generally believed to be significant for the stability of certain projects involving rocks, such as nuclear waste storage and geothermal resource exploitation. In this paper, veined marble specimens were treated to high temperature treatment and then used in conventional triaxial compression tests to investigate the effect of temperature, confining pressure, and vein angle on strength and deformation behaviors. The results show that the strength and deformation parameters of the veined marble specimens changed with the temperature, presenting a critical temperature of 600℃. The triaxial compression strength of a horizontal vein(β = 90°) is obviously larger than that of a vertical vein(β = 0°). The triaxial compression strength,elasticity modulus, and secant modulus have an approximately linear relation to the confining pressure. Finally,Mohr–Coulomb and Hoek–Brown criteria were respectively used to analyze the effect of confining pressure on triaxial compression strength. | Haijian Su Hongwen Jing Qian Yin Liyuan Yu Yingchao Wang Xingjie Wu | 2017 | Acta Mechanica Sinica2017，33，5： | 5 |
| 6 | Serine metabolism orchestrates macrophage polarization by regulating the IGF1–p38 axis显示文摘Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway or by exogenous serine and glycine restriction, robustly enhances the polarization of interferon-γ-activated macrophages (M(IFN-γ)) but suppresses that of interleukin-4-activated macrophages (M(IL-4)) both in vitro and in vivo. Mechanistically, serine metabolism deficiency increases the expression of IGF1 by reducing the promoter abundance of S-adenosyl methionine-dependent histone H3 lysine 27 trimethylation. IGF1 then activates the p38-dependent JAK–STAT1 axis to promote M(IFN-γ) polarization and suppress STAT6-mediated M(IL-4) activation. This study reveals a new mechanism by which serine metabolism orchestrates macrophage polarization and suggests the manipulation of serine metabolism as a therapeutic strategy for macrophage-mediated immune diseases. | Xiao Shan Penghui Hu Lina Ni Long Shen Yanan Zhang Zemin Ji Yan Cui Meihua Guo Haoan Wang Liyuan Ran Kun Yang Ting Wang Lei Wang Bin Chen Zhi Yao Vingjie Wu Qiujing Yu | 2022 | Cellular & Molecular Immunology2022，19，11： | 5 |
| 7 | The landscape of aging显示文摘Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels. | Yusheng Cai Wei Song Jiaming Li Ying Jing Chuqian Liang Liyuan Zhang Xia Zhang Wenhui Zhang Beibei Liu Yongpan An Jingyi Li Baixue Tang Siyu Pei Xueying Wu Yuxuan Liu Cheng-Le Zhuang Yilin Ying Xuefeng Dou Yu Chen Fu-Hui Xiao Dingfeng Li Ruici Yang Ya Zhao Yang Wang Lihui Wang Yujing Li Shuai Ma Si Wang Xiaoyuan Song Jie Ren Liang Zhang Jun Wang Weiqi Zhang Zhengwei Xie Jing Qu Jianwei Wang Yichuan Xiao Ye Tian Gelin Wang Ping Hu Jing Ye Yu Sun Zhiyong Mao Qing-Peng Kong Qiang Liu Weiguo Zou Xiao-Li Tian Zhi-Xiong Xiao Yong Liu Jun-Ping Liu Moshi Song Jing-Dong J.Han Guang-Hui Liu | 2022 | Science China(Life Sciences)2022，65，12： | 4 |
| 8 | A 256×256 time-of-flight image sensor based on center-tap demodulation pixel structure显示文摘This paper proposes a 256×256 time-of-flight(TOF) image sensor based on the center-tap(CT)demodulation pixel structure. The image sensor can capture both the two-dimensional(2D) high speed image and the three-dimensional(3D) depth image. The CT pixel consists of two split pinned photodiode(PPD)regions and two pairs of transfer transistors. The transfer transistors adopt a non-uniform doped channel(NUDC) structure, which can increase the electron transfer speed along the transfer channel and eliminate the image lag for high speed imaging. The pixel size is 10 μm×10 μm, and we design the implementation process of the pixel to increase the electron transfer speed. The sensor is fabricated in a 0.18 μm 1P5 M CMOS image sensor process. Test results show that it can capture the 430-fps intensity image and the 90-fps depth image in two different imaging modes. The rectified non-linearity within the 1.0–7.5 m depth measurement range achieves less than 3 cm, and the measurement accuracy achieves 4.0 cm at 2.5 m, corresponding to the relative error of1.6%. | Zhe CHEN Shan DI Zhongxiang CAO Qi QIN Fei JI Liyuan LIU Nanjian WU | 2016 | Science China(Information Sciences)2016，59，4： | 4 |
| 9 | Acetate-assistant efficient cation-exchange of halide perovskite nanocrystals to boost the photocatalytic CO_(2) reduction显示文摘The judicious implantation of active metal cations into the surface of semiconductor nanocrystal(NC)through cation-exchange is one of the facile and viable strategies to enhance the activity of catalysts for photocatalytic CO_(2)reduction,by shortening the transfer pathway of photogenerated carriers and increasing the active sites simultaneously.However,cation-exchange is hard to achieve for halide perovskite NCs owing to the stable octahedron of[PbX6]4−with strong interaction between halogen and lead.Herein,we report a facile method to overcome this obstacle by replacing partial Br−with acetate(Ac−)to generate CsPbBr_(3) NC(coded as CsPbBr_(3−x)Ac_(x)).A small amount of Ac−instead of Br−does not change the crystal structure of halide perovskite.Owing to the weaker interaction between acetate and lead in comparison with bromide,the corresponding octahedron structure containing acetate in CsPbBr_(3−x)Ac_(x) can be easily opened to realize efficient cation-exchange with Ni^(2+) ions.The resulting high loading amount of Ni^(2+) as active site endows CsPbBr_(3−x)Ac_(x) with an improved performance for photocatalytic CO_(2)reduction under visible light irradiation,exhibiting a significantly increased CO yield of 44.09μmol·g^(−1)·h^(−1),which is over 8 and 3 times higher than those of traditional pristine CsPbBr_(3) and nickel doped CsPbBr_(3) NC,respectively.This work provides a critical solution for the efficient metal doping of low-cost halide perovskite NCs to enhance their photocatalytic activity,promoting their practical applications in the field of photocatalysis. | Jialin Cheng Yanfei Mu Liyuan Wu Zhaolei Liu Ke Su Guangxing Dong Min Zhang Tongbu Lu | 2022 | Nano Research2022，15，3： | 3 |
| 10 | Fiber-shaped organic electrochemical transistors for biochemical detections with high sensitivity and stability显示文摘Precise and continuous monitoring of biochemicals by biosensors assists to understand physiological functions for various diagnostics and therapeutic applications.For implanted biosensors,small size and flexibility are essential for minimizing tissue damage and achieving accurate detection.However,the active surface area of sensor decreases as the sensor becomes smaller,which will increase the impedance and decrease the signal to noise ratio,resulting in a poor detection limit.Taking advantages of local amplification effect,organic electrochemical transistors(OECTs)constitute promising candidates for high-sensitive monitoring.However,their detections in deep tissues are rarely reported.Herein,we report a family of implantable,fiber-shaped all-in-one OECTs based on carbon nanotube fibers for versatile biochemical detection including H2O2,glucose,dopamine and glutamate.These fiber-shaped OECTs demonstrated high sensitivity,dynamical stability in physiological environment and antiinterference capability.After implantation in mouse brain,7-day dopamine monitoring in vivo was realized for the first time.These fiber-shaped OECTs could be great additions to the'life science'tool box and represent promising avenue for biomedical monitoring. | Xiaoying Wu Jianyou Feng Jue Deng Zhichang Cui Liyuan Wang Songlin Xie Chuanrui Chen Chengqiang Tang Zhengqi Han Hongbo Yu Xuemei Sun Huisheng Peng | 2020 | Science China Chemistry2020，63，9： | 3 |
| 11 | High-risk phenotypes of genetic disease in a Neonatal Intensive Care Unit population显示文摘To the Editor: Genetic diseases contribute to 35% of deaths during the first year of life and are a significant cause of intensive care.[1] A previous study based on the China Neonatal Genomes Project investigated the genetic causes of early infant deaths and found that >25% of deceased neonates with genetic diagnoses can be cured if diagnosed in time.[2] Therefore, it is crucial to target and diagnose neonates with genetic diseases as early as possible. According to our experience, the typical phenotypes, such as special facial features or multiple congenital anomalies (MCAs), indicate a high risk of genetic disease and lead physicians to perform genetic testing in neonates as early as possible. However, in practice, infants without typical phenotypes typically undergo a long and costly diagnostic process before genetic diagnoses are confirmed. Moreover, a recent survey by the American College of Medical Genetics and Genomics (ACMG) and other national professional organizations indicated that there are insufficient numbers of qualified geneticists to fulfil genetic service needs.[3] The ACMG published the general clinical features for genetic testing indications. For example, patients with phenotypes or family history data that strongly implicate a genetic cause may undergo genetic testing.[1] However, the study indicated that many genetic conditions arise de novo or are inherited with no family history.[1] A previous study attempted to apply the non-phenotype-driven panel approach in neonates admitted to the neonate intensive care unit (NICU).[4] However, at present, the diagnostic yield is only 3.45% (1/29).[4] In addition, the economic and ethical issues associated with genomic screening remain challenging. Therefore, the available indications for genetic testing may improve the management of genetic diseases. | Tiantian Xiao Qi Ni Huiyao Chen Huijun Wang Lin Yang Bingbing Wu Yun Cao Guoqiang Cheng Laishuan Wang Liyuan Hu Hongfang Mei Yulan Lu Mengchun Gong Xinran Dong Wenhao Zhou | 2022 | Chinese Medical Journal2022，，5： | 2 |
| 12 | Hepatitis B virus evades immune recognition via RNA adenosine deaminase ADAR1-mediated viral RNA editing in hepatocytes显示文摘HBV is considered as a“stealth”virus that does not invoke interferon(IFN)responses;however,the mechanisms by which HBV bypasses innate immune recognition are poorly understood.In this study,we identified adenosine deaminases acting on RNA 1(ADAR1),which is a key factor in HBV evasion from IFN responses in hepatocytes.Mechanically,ADAR1 interacted with HBV RNAs and deaminated adenosine(A)to generate inosine(I),which disrupted host immune recognition and thus promoted HBV replication.Loss of ADAR1 or its deficient deaminase activity promoted IFN responses and inhibited HBV replication in hepatocytes,and blocking the IFN signaling pathways released the inhibition of HBV replication caused by ADAR1 deficiency.Notably,the HBV X protein(HBx)transcriptionally promoted ADAR1 expression to increase the threshold required to trigger intrinsic immune activation,which in turn enhanced HBV escape from immune recognition,leading to persistent infection.Supplementation with 8-azaadenosine,an ADAR1 inhibitor,efficiently enhanced liver immune activation to promote HBV clearance in vivo and in vitro.Taken together,our results delineate a molecular mechanism by which HBx promotes ADAR1-derived HBV immune escape and suggest a targeted therapeutic intervention for HBV infection. | Liyuan Wang Yang Sun Xiaojia Song Zehua Wang Yankun Zhang Ying Zhao Xueqi Peng Xiaodong Zhang Chunyang Li Chengjiang Gao Nailin Li Lifen Gao Xiaohong Liang Zhuanchang Wu Chunhong Ma | 2021 | Cellular & Molecular Immunology2021，18，8： | 2 |
| 13 | The Main Progress of Perovskite Solar Cells in 2020-2021显示文摘Perovskite solar cells(PSCs)emerging as a promising photovoltaic technology with high efficiency and low manufacturing cost have attracted the attention from all over the world.Both the efficiency and stability of PSCs have increased steadily in recent years,and the research on reducing lead leakage and developing eco-friendly lead-free perovskites pushes forward the commercialization of PSCs step by step.This review summarizes the main progress of PSCs in 2020 and 2021 from the aspects of efficiency,stability,perovskite-based tandem devices,and lead-free PSCs.Moreover,a brief discussion on the development of PSC modules and its challenges toward practical application is provided. | Tianhao Wu Zhenzhen Qin Yanbo Wang Yongzhen Wu Wei Chen Shufang Zhang Molang Cai Songyuan Dai Jing Zhang Jian Liu Zhongmin Zhou Xiao Liu Hiroshi Segawa Hairen Tan Qunwei Tang Junfeng Fang Yaowen Li Liming Ding Zhijun Ning Yabing Qi Yiqiang Zhang Liyuan Han | 2021 | Nano-Micro Letters2021，13，10： | 2 |
| 14 | Co-effect of Mechanical Ball-milling and Microenvironmental Polarity on Morphology and Properties of Nanocellulose显示文摘Cellulose is a linear polymer consisting of D-anhydroglucose units joined by β-1,4-glycosidic linkages. The densely packed cellulose molecular chain forms crystalline cellulose through strong hydrogen bonding. Owing to its chemical tunability and excellent mechanical resistance, nanocellulose is widely used in everyday life and the industrial sector. In this work, cellulose materials were nanoprocessed by mechanical ball-milling(1) in polar solvents(N,N-dimethylformamide or dimethyl sulfoxide) with esterification or(2) in hydrophobic agents(polydimethylsiloxane or polytetrafluoroethylene) with different molecular weights. Cellulose nanofibers and nanosheets with different hydrophilic and hydrophobic properties were obtained, and the mechanism of cellulose disintegration along a crystallographic plane induced by mechanical force and the polarity condition were discussed. This work affords a new way to manipulate the morphology and properties of nanocellulose. | Chao Wang LiYuan Li MengMeng Zhao Pei Huang Shigenori Kuga Min Wu Yong Huang | 2017 | Paper And Biomaterials2017，2，4： | 2 |
| 15 | Phylogenetic Analysis of the Dengue Virus Strains Causing the 2019 Dengue Fever Outbreak in Hainan,China显示文摘Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp.mosquitos,causing the acute infectious disease,dengue fever(DF).Until 2019,no dengue outbreak had been reported in Hainan Province for over 20 years.However,in early September of 2019,an increasing number of infected cases appeared and the DF outbreak lasted for over one month in Haikou City,Hainan Province.In our study,we collected 97 plasma samples from DF patients at three hospitals,as well as 1585 mosquito larvae samples from puddles in different areas of Haikou.There were 49(50.5%)plasma samples found to be strongly positive and 9(9.3%)plasma samples were weakly positive against the NS1 antigen.We discovered DENV both in the patient's plasma samples and mosquito larvae samples,and isolated the virus from C6/36 cells inoculated with the acute phase serum of patients.Phylogenetic analysis revealed that the new strains were the most closely related to the epidemic strain in the southern regions of China,belonging to lineageⅣ,genotypeⅠ,DENV-1.Compared to the seven closest strains from neighboring countries and provinces,a total of 18 amino acid mutations occurred in the coding sequences(CDS)of the new isolated strain,DENV1 HMU-HKU-2.Our data shows that dengue virus is re-emerged in Hainan,and pose new threats for public health.Thus regular molecular epidemiological surveillance is necessary for control and prevention of DENV transmission. | Jiang Du Liyuan Zhang Xiaoyuan Hu Ruoyan Peng Gaoyu Wang Yi Huang Wenqi Wang Kunliang Wu Qiang Wang Haoxiang Su Fan Yang Yun Zhang Chuanning Tang Xiuji Cui Lina Niu Gang Lu Meifang Xiao Yongguo Du Feifei Yin | 2021 | Virologica Sinica2021，36，4： | 2 |
| 16 | Overexpression of EphA2 correlates with epithelial–mesenchymal transition-related proteins in gastric cancer and their prognostic importance for postoperative patients显示文摘 | Futao Hou Weijie Yuan Jin Huang Liyuan Qian Zhikang Chen Jie Ge Shaobin Wu Jinxiang Chen Jixu Wang Zihua Chen | 2012 | Medical Oncology2012，，4： | 2 |
| 17 | The establishment of polypeptide PSMA-targeted chimericantigen receptor-engineered natural killer cells forcastration-resistant prostate cancer and the induction of ferroptosis-related cell death显示文摘Background:The mortality of castration-resistant prostate cancer(CRPC)is high due to lack of an effective treatment.Chimeric antigen receptor(CAR)-based therapy is a promising immunotherapeutic strategy.Here,we aimed to design a novel CAR-natural killer(NK)cells with a clinically significant tumoricidal effect on CRPC.Methods:We constructed novel CAR-NK92MI cells with a CD244-based recombinant lentiviral vector.Different intracellular segments(CD244,NKG2D,or CD3ζ)were screened to identify the best candidate according to cell lysis assay and CD107a expression levels.To enhance the affinity of the CAR to the tumor antigen,we compared an antibody specific for prostate-specific membrane antigen(anti-PSMA)with PSMA-targeted polypeptide(p-PSMA),which was screened by phage display combinatorial library.Then,CAR-NK92MI cells with both a high affinity for PSMA and a strong tumoricidal capacity were generated.In addition,we verified their tumor-killing effect in vitro and in vivo.The release of cytokine by NK92MI cells was compared with that by CAR-NK92MI cells through flow cytometry and enzyme-linked immunosorbent assay.Moreover,ferroptosis-related cell death was explored as a possible underlying mechanism.Results:Three different CAR intracellular regions CAR1(CD244),CAR2(CD244,NKG2D)and CAR3(CD244,NKG2D,and CD3ζ)were constructed.CAR2 was chosen to confer a stronger tumoricidal ability on CAR-NK92MI cells.Compared with anti-PSMA,p-PSMA exhibited enhanced affinity for the tumor antigen.Thus,p-PSMA-CAR-NK92MI cells,which expressed CAR with a polypeptide-based antigen-binding region,an intracellular CD244 and a NKG2D costimulatory domain,were generated.They could selectively and successfully kill PSMA+target cells and exhibited specific lysis rate of 73.19%for PSMA-positive C4-2 cells and 33.04%for PSMA-negative PC3 cells.Additionally,p-PSMA-CAR-NK92MI cells had significantly higher concentrations of IFN-γ,TNF-αand granzyme B than NK92MI cells.In a CRPC cancer xenograft model,p-PSMA-CAR-NK92MI cells significantly inhibited tumor growth and exerted a more consistent killing effect than NK92MI cells.Moreover,ferroptosis is a potential mechanism through which CAR-NK92MI cells attack cancer cells,and is triggered by IFN-γ.Conclusions:p-PSMA-CAR-NK92MI cells can effectively kill CRPCPSMA+cells in vitro and in vivo.This strategy may provide additional treatment options for patients with CRPC. | Liyuan Wu Fei Liu Le Yin Fangming Wang Hui Shi Qinxin Zhao Feiya Yang Dong Chen Xiying Dong Yuchun Gu Nianzeng Xing | 2022 | Cancer Communications2022，42，8： | 2 |
| 18 | A novel terbium metal-organic framework for luminescence sensing of pyridine:Synthesis,structure,selectivity,sensitivity and recyclability显示文摘One novel two-dimensional(2D)terbium-based framework[Tb(L2-)(Ac)(DMA)]n(1)(H2 L=4’-(3,5-dicarboxyphenyl)-4,2’:6’,4'-terpyridine)was successfully isolated and structurally characterized.The structural analysis reveals that two Tb3+ions in 1 are bridged by twoη1:η1:μ2 carboxylates from L2-to form a binuclear unit,which is further linked by L2-to generate a 2D layer with kgd topology.Moreover,1 displays excellent thermostability and extensive solvent stability.Luminescent measurements reveal that 1 can be used as a recyclable luminescent probe for detecting pyridine with the lowest detection lim it of 0.12 vol%,and the luminescent mechanism is also discussed. | Ying Shi Liyuan He Xinxin Wang Zhilei Wu Ning Gao Huan Zhang Wenmin Wang Jianzhong Cui | 2020 | Journal of Rare Earths2020，38，11： | 2 |
| 19 | Droplet Manipulation and Crystallization Regulation in Inkjet-Printed Perovskite Film Formation显示文摘Emerging organic–inorganic metal halide perovskite materials have become the focus of the optoelectronics research community owing to their excellent photoelectric properties.Nevertheless,challenges still exist for transferring the lab-made devices to largearea industrial modules.Inkjet printing(IJP)technology provides a promising way to fill the gap because of its precise droplet control and uniform large-scale deposition functions.Hence,an in-depth understanding of inkjet-printed perovskite films in terms of droplet manipulation and crystallization regulation is critical for upscaling the perovskite devices to commercial usage.In this review,we give an overview of inkjet-printed high-quality perovskite films and provide guidelines on inkjet-printing large-scale highperformance perovskite devices.First,we analyze theories of droplet formation and perovskite nucleation/crystallization dynamics and then focus on summarizing the perovskite film-formation strategies via IJP,in the aspects of ink engineering,the printing process,and posttreatment.Furthermore,we review the recent advances of inkjet-printed perovskite films on optoelectronic devices,such as perovskite solar cells,perovskite light-emitting diodes,and perovskite photodetectors.Finally,we highlight the“Trilogy Strategies,”including ink engineering,printing process,and posttreatment for printing high-quality perovskite films. | Yajie Cheng Hangjuan Wu Junjie Ma Pengwei Li Zhenkun Gu Shuangquan Zang Liyuan Han Yiqiang Zhang Yanlin Song | 2022 | CCS Chemistry2022，4，5： | 1 |
| 20 | Adsorption of Cu ( II )-EDTA chelates on tri-ammonium-functionalized mesoporo- us silica from aqueous solution显示文摘 | Wu Liyuan Wang Hongjie Lan Huaehun | 2013 | Separation and Purification Teehnology2013，117，： | 1 |
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