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| 1 | Interaction of Hsp40 with influenza virus M2 protein: implications for PKR signaling pathway显示文摘Influenza virus contains three integral membrane proteins:haemagglutinin,neuraminidase,and matrix protein(M1 and M2).Among them,M2 protein functions as an ion channel,important for virus uncoating in endosomes of virus-infected cells and essential for virus replication.In an effort to explore potential new functions of M2 in the virus life cycle,we used yeast two-hybrid system to search for M2-associated cellular proteins.One of the positive clones was identified as human Hsp40/Hdj1,a DnaJ/Hsp40 family protein.Here,we report that both BM2(M2 of influenza B virus)and A/M2(M2 of influenza A virus)interacted with Hsp40 in vitro and in vivo.The region of M2-Hsp40 interaction has been mapped to the CTD1 domain of Hsp40.Hsp40 has been reported to be a regulator of PKR signaling pathway by interacting with p58^(IPK) that is a cellular inhibitor of PKR.PKR is a crucial component of the host defense response against virus infection.We therefore attempted to understand the relationship among M2,Hsp40 and p58^(IPK) by further experimentation.The results demonstrated that both A/M2 and BM2 are able to bind to p58^(IPK)in vitro and in vivo and enhance PKR autophosphorylation probably via forming a stable complex with Hsp40 and P58^(IPK),and consequently induce cell death.These results suggest that influenza virus M2 protein is involved in p58^(IPK)mediated PKR regulation during influenza virus infection,therefore affecting infected-cell life cycle and virus replication. | Zhenhong Guan Di Liu Shuofu Mi Jie Zhang Qinong Ye Ming Wang George F.Gao Jinghua Yan | 2010 | Protein & Cell2010，1，10： | 11 |
| 2 | Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis显示文摘 | Xu Xiaojie Fan Zhongyi Kang Lei Han Juqiang Jiang Chengying Zheng Xiaofei Zhu Ziman Jiao Huabo Lin Jing Jiang Kai Ding Lihua Zhang Hao Cheng Long Fu Hanjiang Song Yi Jiang Ying Liu Jiahong Wang Rongfu Du Nan Ye Qinong | 2013 | Journal of Clinical Investigation2013，，2： | 7 |
| 3 | Human pescadillo induces large-scale chromatin unfolding显示文摘The human pescadillo gene encodes a protein with a BRCT domain. Pescadillo plays an important role in DNA synthesis, cell proliferation and transformation. Since BRCT do- mains have been shown to induce chromatin large-scale unfolding, we tested the role of Pesca- dillo in regulation of large-scale chromatin unfolding. To this end, we isolated the coding region of Pescadillo from human mammary MCF10A cells. Compared with the reported sequence, the isolated Pescadillo contains in-frame deletion from amino acid 580 to 582. Targeting the Pesca- dillo to an amplified, lac operator-containing chromosome region in the mammalian genome re- sults in large-scale chromatin decondensation. This unfolding activity maps to the BRCT domain of Pescadillo. These data provide a new clue to understanding the vital role of Pescadillo. | ZHANG Hao FANG Yan HUANG Cuifen YANG Xiao YE Qinong | 2005 | Science China(Life Sciences)2005，48，3： | 4 |
| 4 | Metabolism and immunity in breast cancer显示文摘Breast cancer is one of the most common malignancies that seriously threaten women’s health.In the process of the malignant transformation of breast cancer,metabolic reprogramming and immune evasion represent the two main fascinating characteristics of cancer and facilitate cancer cell proliferation.Breast cancer cells generate energy through increased glucose metabolism.Lipid metabolism contributes to biological signal pathways and forms cell membranes except energy generation.Amino acids act as basic protein units and metabolic regulators in supporting cell growth.For tumor-associated immunity,poor immunogenicity and heightened immunosuppression cause breast cancer cells to evade the host’s immune system.For the past few years,the complex mechanisms of metabolic reprogramming and immune evasion are deeply investigated,and the genes involved in these processes are used as clinical therapeutic targets for breast cancer.Here,we review the recent findings related to abnormal metabolism and immune characteristics,regulatory mechanisms,their links,and relevant therapeutic strategies. | Deyu Zhang Xiaojie Xu Qinong Ye | 2021 | Frontiers of Medicine2021，15，2： | 3 |
| 5 | p53-dependent upregulation of PIG3 transcription by γ-ray irradiation and its interaction with KAP1 in responding to DNA damage显示文摘PIG3 (p53-inducible gene 3), originally identified as one of a set of genes induced by p53 before the onset of apoptosis, was assumed to contribute to early cellular response to DNA damage. Here, we studied the relation between p53 status and the increased expression of PIG3 by ionizing radiation (IR), and the related clues regarding the involvement of PIG3 in the cellular response to IR-induced DNA damage signaling. We demonstrated that the pentanucleotide microsatellite sequence was responsible for the p53-dependent induction of PIG3 transcription after irradiation, while sequence upstream of PIG3 promoter could maintain the basal level of expression which was not inducible by irradiation. The interaction of PIG3 and the KRAB-ZFP-associated protein 1 (KAP1), a DNA damage response protein, was revealed. PIG3 nucleus foci were formed 15 min after γ-ray irradiation, and which were found to partially colocalize with the phospho-KAP-1 foci as well as γ-H2AX foci. Although the lac operator tagged EGFP based reporter system revealed that PIG3 does not remodel chromatin in large scale in the cells under normal growing condition, it indeed prompted the chromatin relaxation in the cellular response to DNA damage signaling. All these data suggest that PIG3 is involved in IR-induced DNA damage response, and which maybe partially attribute to its interaction with KAP1. | QIN Xia ZHANG ShiMeng LI Bingi LIU XiaoDan HE XingPeng SHANG ZengFu XU QinZhi ZHAO ZengQiang YE QiNong ZHOU PingKun | 2011 | Chinese Science Bulletin2011，56，30： | 2 |
| 6 | SARS-CoV-2 spike L452R mutation increases Omicron variant fusogenicity and infectivity as well as host glycolysis显示文摘Dear Editor,The SARS-CoV-2 Omicron variant has rapidly displaced the Delta variant and spread across the world.The Omicron variant harbors over 60 mutations,and 15 of the mutations are located in the receptor-binding domain(RBD).1 Compared with parental virus and previous variants,the Omicron variant is characterized by decreased hospitalization rates and less severe disease in patients. | Yanan Zhang Ting Zhang Yihui Fang Jie Liu Qinong Ye Lihua Ding | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 2 |
| 7 | Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-[beta]-like signaling pathway显示文摘 | Ding Lihua Wang Zhaoyun Yan Jinghua Yang Xiao Liu Aijun Qiu Weiyi Zhu Jianhua Han Juqiang Zhang Hao Lin Jing Cheng Long Qin Xi Niu Chang Yuan Bin Wang Xiaohui Zhu Cui Zhou Yan Li Jiezhi Song Haifeng Huang Cuifen Ye Qinong | 2009 | Journal of Clinical Investigation2009，，2： | 1 |
| 8 | The molecular mechanism and potential role of heat shock-induced p53 protein accumulation显示文摘 | Juqiang Han Xiaojie Xu Hongzhen Qin Anheng Liu Zhongyi Fan Lei Kang Jing Fu Jiahong Liu Qinong Ye | 2013 | Molecular and Cellular Biochemistry2013，，1： | 1 |
| 9 | MiR-495 functions as an adjuvant to radiation therapy by reducing the radiation-induced bystander effect显示文摘 | Jie Fu Mengmeng Jiang Meng Zhang Jing Zhang Yu Wang Shensi Xiang Xiaojie Xu Qinong Ye Haifeng Song | 2016 | Acta Biochimica et Biophysica Sinica2016，48，11： | 1 |
| 10 | Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis显示文摘 | Xu Xiaojie Fan Zhongyi Kang Lei Han Juqiang Jiang Chengying Zheng Xiaofei Zhu Ziman Jiao Huabo Lin Jing Jiang Kai Ding Lihua Zhang Hao Cheng Long Fu Hanjiang Song Yi Jiang Ying Liu Jiahong Wang Rongfu Du Nan Ye Qinong | 2013 | Journal of Clinical Investigation2013，，2： | 1 |
| 11 | BRCAl-induced largescale chromatin unfolding and allele-specific effects of cancerpredisposing mutations显示文摘 | Ye Qinong Hu YF Zhong HG | 2001 | J of Cell Biology2001，155，： | 1 |
| 12 | HUNK inhibits cargo uptake and lysosomal traffic in the caveolar pathway via the AGAP3/ARF6显示文摘Endocytosis is a crucial cellular process that takes up cargos by enclosing them in membrane-bound vesicles,and transports cargos to different parts of the cell,such as the lysosomes[1].Endocytosis is classified into several mechanistically and morphologically pathways,including clathrin-mediated endocytosis(CME)and caveolae-mediated endocytosis(CavME)[1,2]. | Siyuan Jiang Xiaoqi Han Tihui Liu Ying He Zidong Zhao Tongfeng Liu Shuwen Cheng Jihang Zhang Liqiang Duan Yajuan Liu Tianyou Cheng Yong Liu Qinong Ye Shan Gao | 2024 | Science Bulletin2024，69，2： | 0 |
| 13 | The ubiquitin ligase E6AP facilitates HDAC6-mediated deacetylation and degradation of tumor suppressors显示文摘Dear Editor,Protein acetylation status can regulate protein stability via the ubiquitin-proteasome pathway,which plays a critical role in the regulation of various cellular functions and becomes a target for cancer therapy.1,2 Histone deacetylase 6(HDAC6)belongs to the class II of the histone deacetylase/acuc/apha family and regulates cancer cell proliferation,invasion,and metastasis.3 Combination of HDAC6 inhibitors(HDAC6i)with proteasome inhibitors(PI)shows synergistic anticancer activity.4 Although many studies reveal how acetyltransferases/deacetylases regulate protein acetylation and subsequent protein ubiquitination and degradation,whether an E3 ubiquitin ligase regulates protein acetylation remains largely unknown.In addition,the mechanisms undelying synergistic anticancer activity of HDAC6i and PI remain poorly understood. | Yanan Zhang Zhida Chen Jing Lin Jie Liu Yahong Lin Huayue Li Yongyi Xi Bo Wei Lihua Ding Qinong Ye | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 14 | A novel aberrant splicing of G_(sa) transcript in human leukemia cell lines显示文摘The α subunit of the stimulatory G protein, Gsα, is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various Gsα mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing of Gsα transcript in human leukemia cell lines is reported. The entire coding region of Gsα gene obtained, referred to as GsαLeu, was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse-transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that,compared with originally reported Gsα, GsαLeu has two in-frame deletions, one in amino acid residues 23—249 and the other in amino acid residues 254—260, suggesting that G(sαLeu) may play an important role in leukemia. The complete coding region of GsαLu was inserted downstream of the thrombin site of pGEX-2T fusion protein expression vector and expressed in the form of GST/G( | YE Qinong, YAO Xiao, WANG Hengliang, ZHANG Shu, LIU Huaitian, SU Guofu, HUANG Cuifen and ZHOU Tingchong Beijing Institute of Biotechnology, Beijing 100071, China Department of Biology, Shaanxi Normal College, Xi’an 710062, China Beijing Medical University, School of Oncology, Beijing 100036, China Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China Beijing Institute of Basic Medicine Sciences , Beijing 100850, China | 1999 | Chinese Science Bulletin1999，44，4： | 0 |
| 15 | DEYA2在肺腺癌中表达升高(英文)显示文摘Objective:Lung cancer has emerged as a leading cause of cancer death in the world.Eyes Absent (EYA) is an important and conserved transcriptional regulator of development.The aim of the present study was to identify the expression of Drosophila Eyes Absent Homologue 2 (EYA2) in non-small cell lung cancer (NSCLC) and to investigate their correlation with clinical parameters.Methods:Fresh,paired lung samples (n=59) of NSCLC were obtained by surgical resection at the Department of Thoracic Surgery of the People's Liberation Army General Hospital.Expression of EYA2 were examined by Western blot and immunohistochemical analysis in specimens of NSCLC and paired normal lung tissue.Clinical data,patho-logic result and Ki67 expression were collected and subsequent correlation with EYA2 expression was analyzed.Results:EYA2 expression was found located in cytoplasm and nucleus,but mostly in cytoplasm.The expression of EYA2 increased in NSCLC by Western blot and immunohistochemistry,which was correlated with histology type,but not correlated with gender,age,pTNM stage,histological differentiation and lymph node metastasis.Compared with normal lung tissue,the expres-sion of EYA2 significantly was up-regulated in lung adenocarcinoma,while no significant difference in lung squamous cell carcinoma.Expression of EYA2 was uncorrelated with expression of Ki67 in NSCLC.Conclusion:Expression of EYA2 was augmented in lung adenocarcinoma.EYA2 is likely participating in tumorigenesis and development of lung adenocarcinoma as transcriptional activator. | Juntang Guo Chaoyang Liang Lihua Ding Naikang Zhou Qinong Ye | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，12： | 0 |
| 16 | Preliminary study on discovery of a novel molecule that interacts with CPP32 using two-hybrid system显示文摘Of ICE protease family, CPP32 (apopain or Yama) plays a central role in different apoptotic pathways. To study the molecules regulating CPP32, yeast two_hybrid system was used to identify proteins (peptides) that interact with CPP32. First, the CPP32 gene was cloned into plasmid vector pGBT9. The resulting recombinant plasmid was designated as pGBT9/CPP. The pGBT9 /CPP plasmid was transformed into the yeast strain HF7C, then the leukemia library was introduced. The transformation mixture was plated on medium lacking Trp, Leu and His in the initial screen. Colonies growing on the selection medium were further assayed for β galactosidase activity. Within 42 Trp +Leu +His + colonies only 5 turned blue in the presence of X_Gal. Plasmid DNA from 5 positive yeast colonies was prepared respectively and used to transform HB101 by electroporation. The transformation mixture was plated on medium lacking Leu to be selected for the library plasmid. Finally, only one library plasmid, designated as pY1, was determined to be truly positive by retransformation of pGBT9/CPP and the library plasmid into HF7C. The inserted cDNA of pY1 encodes a peptide of 15 amino acids, suggesting that it may be the domain interacting with CPP32. | Ye Qinong Yao Xiao Wang Hengliang Su Guofu Huang Cuifen Zhou Tingchong | 1998 | Chinese Science Bulletin1998，43，4： | 0 |
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