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| 1 | Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum-organelle interactions显示文摘The endoplasmic reticulum(ER)is a contiguous and complicated membrane network in eukaryotic cells,and membrane contact sites(MCSs)between the ER and other organelles perform vital cellular functions,including lipid homeostasis,metabolite exchange,calcium level regulation,and organelle division.Here,we establish a whole pipeline to reconstruct all ER,mitochondria,lipid droplets,lysosomes,peroxisomes,and nuclei by automated tape-collecting ultramicrotome scanning electron microscopy and deep learning techniques,which generates an unprecedented 3D model for mapping liver samples.Furthermore,the morphology of various organelles and the MCSs between the ER and other organelles are systematically analyzed.We found that the ER presents with predominantly flat cisternae and is knitted tightly all throughout the intracellular space and around other organelles.In addition,the ER has a smaller volume-to-membrane surface area ratio than other organelles,which suggests that the ER could be more suited for functions that require a large membrane surface area.Our data also indicate that ER-mitochondria contacts are particularly abundant,especially for branched mitochondria.Our study provides 3D reconstructions of various organelles in liver samples together with important fundamental information for biochemical and functional studies in the liver. | Yi Jiang Linlin Li Xi Chen Jiazheng Liu Jingbin Yuan Qiwei Xie Hua Han | 2021 | Journal of Molecular Cell Biology2021，13，9： | 2 |
| 2 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 3 | Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H 2 SO 4 pretreatments in Miscanthus显示文摘 | Fengcheng Li Shuangfeng Ren Wei Zhang Zhengdan Xu Guosheng Xie Yan Chen Yuanyuan Tu Qing Li Shiguang Zhou Yu Li Fen Tu Lin Liu Yanting Wang Jianxiong Jiang Jingping Qin Shizhong Li Qiwei Li Hai-Chun Jing Fasong Zhou Neal Gutterson Liangcai Peng | 2012 | Bioresource Technology2012，，： | 1 |
| 4 | Genetic Research and Utilization Reference of Saccharum spontaneum L.显示文摘[Objectives]The genetic characteristics of wild germplasm resources of Saccharum spontaneum L.were further investigated to provide a scientific theoretical basis for the improved hybridization of sugarcane varieties.[Methods]The first flowering date data of 112 wild resources were statistically analyzed by broad heritability for 3 consecutive years,and pollen quantity and yield were investigated to provide reference for hybridization.[Results]The broad heritability of the first flowering date of S.spontaneum was 0.079.The differences in the first flowering date between the S.spontaneum resources were in the range of 4-49 d,with an average of 13.65 d.The inter-annual changes within 20 d accounted for 90 %,and only 4.5% exceeded 30 d.The first flowering dates of some S.spontaneum resources were obviously postponed after being treated.[Conclusions]In the hybridization season,the hybridization plan must be adjusted according to the actual situation of the flowering period to make full use of it. | Jiale JI Qinnan WANG Yongsheng QIU Cheng FU Qiwei WU Houxiang HU Feng ZHOU Chuiming ZHANG Huanying XU Jing XIE | 2019 | Agricultural Biotechnology2019，8，3： | 1 |
| 5 | Pilot Postoperative lleus Study of Escin in Cancer Patients After Colorectal Surgery显示文摘 | Qiwei Xie Xianglong Zong Baoming Ge | 2009 | World journal of surgery2009，33，5： | 1 |
| 6 | EMD Sifting based on bandwidth显示文摘 | Xuan Bo Xie Qiwei Peng Silong | 2007 | IEEE Signal Processing Letters2007，14，8： | 1 |
| 7 | Pilot Postoperative Ileus Study of Escin in Cancer Patients After Colorectal Surgery显示文摘 | Qiwei Xie Xianglong Zong Baoming Ge Shan Wang Jiafu Ji Yingjiang Ye Lili Pan | 2009 | World Journal of Surgery2009，，： | 1 |
| 8 | AHA1 upregulates IDH1 and metabolic activity to promote growth and metastasis and predicts prognosis in osteosarcoma显示文摘Osteosarcoma(OS)is the most common primary malignant bone tumor in children and adolescents.Although activator of HSP90 ATPase activity 1(AHA1)is reported to be a potential oncogene,its role in osteosarcoma progression remains largely unclear.Since metabolism reprogramming is involved in tumorigenesis and cancer metastasis,the relationship between AHA1 and cancer metabolism is unknown.In this study,we found that AHA1 is significantly overexpressed in osteosarcoma and related to the prognosis of osteosarcoma patients.AHA1 promotes the growth and metastasis of osteosarcoma both in vitro and in vivo.Mechanistically,AHA1 upregulates the metabolic activity to meet cellular bioenergetic needs in osteosarcoma.Notably,we identifed that isocitrate dehydrogenase 1(IDH1)is a novel client protein of Hsp90 AHA1.Furthermore,the IDH1 protein level was positively correlated with AHA1 in osteosarcoma.And IDH1 overexpression could partially reverse the effect of AHA1 knockdown on cell growth and migration of osteosarcoma.Moreover,high IDH1 level was also associated with poor prognosis of osteosarcoma patients.This study demonstrates that AHA1 positively regulates IDH1 and metabolic activity to promote osteosarcoma growth and metastasis,which provides novel prognostic biomarkers and promising therapeutic targets for osteosarcoma patients. | Diwei Zheng Weihai Liu Wenlin Xie Guanyu Huang Qiwei Jiang Yang Yang Jiarong Huang Zihao Xing Mengling Yuan Mengning Wei Yao Li Junqiang Yin Jingnan Shen Zhi Shi | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 1 |
| 9 | Aligned Organization of Synapses and Mitochondria in Auditory Hair Cells显示文摘Recent studies have revealed great functional and structural heterogeneity in the ribbon-type synapses at the basolateral pole of the isopotential inner hair cell(IHC).This feature is believed to be critical for audition over a wide dynamic range,but whether the spatial gradient of ribbon morphology is fine-tuned in each IHC and how the mitochondrial network is organized to meet local energy demands of synaptic transmission remain unclear.By means of three-dimensional electron microscopy and artificial intelligence-based algorithms,we demonstrated the cell-wide structural quantification of ribbons and mitochondria in mature mid-cochlear IHCs of mice.We found that adjacent IHCs in staggered pairs differ substantially in cell body shape and ribbon morphology gradient as well as mitochondrial organization.Moreover,our analysis argues for a location-specific arrangement of correlated ribbon and mitochondrial function at the basolateral IHC pole. | Jing Liu Shengxiong Wang Yan Lu Haoyu Wang Fangfang Wang Miaoxin Qiu Qiwei Xie Hua Han Yunfeng Hua | 2022 | Neuroscience Bulletin2022，38，3： | 1 |
| 10 | Detector development at the Back-n white neutron source显示文摘Purpose Back-n is a white neutron beamline at China spallation neutron source,which was established in the year of 2018.It is a powerful facility for nuclear data measurement,neutron detector calibration,and radiation effect research.Method A series of detectors were built for different experiments,including beam monitoring,beam profile measurement,neutron induced secondaries(fission fragments,light charged particles and gamma)cross section measurement,and neutron resonance radiography,etc.A common digitization electronics and a cluster-based DAQ were developed for these detector systems.Most detectors have been employed at Back-n and serviced for experiments from the beginning of the beamline running.Results and conclusion As an overview of detectors of Back-n,the details of the detector design and the experiment performing are described in this paper.Some developing systems,e.g.,MTPC and B-MCP,are also included. | Fan Ruirui Li Qiang Bao Jie Li Yang Liu Rong Jiang Wei Ren Jie Zhang Qiwei Cao Ping Gu Minhao Ren Zhizhou Yi Han Tang Jingyu An Qi Bai Haofan Bai Jiangbo Chen Qiping Chen Yonghao Chen Zhen Cui Zengqi Fan Anchuan Feng Changqing Feng Fanzhen Gao Keqing Han Changcai Han Zijie He Guozhu He Yongcheng Hong Yang Hu Yiwei Huang Hanxiong Jia Weihua Jiang Haoyu Jiang Zhijie Jin Zhengyao Kang Ling Li Bo Li Chao Li Gong Li Jiawen Li Xiao Liu Jie Liu Shubin Luan Guangyuan Ning Changjun Qi Binbin Ruan Xichao Song Zhaohui Sun Kang Tan Zhixin Tang Shengda Wang Pengcheng Wang Zhaohui Wen Zhongwei Wu Xiaoguang Wu Xuan Xie Likun Yang Yiwei Yu Yongji Zhang Guohui Zhang Linhao Zhang Mohan Zhang Xianpeng Zhang Yuliang Zhang Yue Zhang Zhiyong Zhao Maoyuan Zhou Luping Zhou Zhihao Zhu Kejun The CSNS Back-n Collaboration | 2023 | Radiation Detection Technology and Methods2023，7，2： | 0 |
| 11 | Application of AHP and DTOPSIS in Sugarcane Breeding显示文摘The weights of ten important traits of sugarcane,including the number of hair groups,plant height,defoliation capability,stalk diameter,ratooning property,stalk number of disease resistance,cane yield,sucrose content and sugar yield,were distributed with the Analytic Hierarchy Process( AHP) method. Ten Yuetang series of sugarcane lines were evaluated by Dynamic Technique for Order Preference by Similarity to Ideal Solution( DTOPSIS). AHP results indicated that the weights of ten traits were 0. 013 4,0. 022 7,0. 030 2,0. 040 8,0. 065 3,0. 088 1,0. 116 2,0. 160 6,0. 198 4 and 0. 264 5,respectively,with random consistency ratio C. R. = 0. 098 1 < 0. 1,suggesting that the distribution of the weights of evaluation indicators was reasonable. Comprehensive evaluation of 10 sugarcane lines by DTOPSIS method combining with the conclusion of AHP showed that the comprehensive performances of Yuetang 03-393 and Yuetang 03-373 were much better than other sugarcane lines,with relative approach degree C i ranking the first and second,respectively,which was basically consistent with the practical survey,suggesting that Yuetang 03-393 and Yuetang 03-373 were suitable for large-scale popularization. The above results confirmed the feasibility of applying AHP and DTOPSIS in comprehensive evaluation of new crop varieties and provided reference for the breeding of new sugarcane lines. | Jiantao WU Junxian YANG Fuye LIU Qiwei LI Wenlong WU Fangyin PAN Jing XIE Yuegui CHEN | 2013 | Agricultural Biotechnology2013，2，5： | 0 |
| 12 | Highly efficient and anti-poisoning single-atom cobalt catalyst for selective hydrogenation of nitroarenes显示文摘Developing non-precious metal catalysts to selectively reduce functionalized nitroarenes with high efficiency is urgently desirable for the production of value-added amines.Herein,we report a novel,efficient,anti-poisoning single-atom cobalt catalyst(Co-NAC)for the highly selective hydrogenation of the nitro to amino group for nitroarenes baring various functional groups,including vinyl,cyano,and halogen.Using a combination of structure characterization techniques,we have confirmed that the cobalt species are predominantly present in the form of four-coordinated Co single sites anchored on nitrogen-assembly carbon(NAC)as the ordered mesoporous support.Co-NAC catalysts enable the full conversion and>99%selectivity with molecular H2 as a green reductant under mild conditions(80℃,2 MPa H2).As for the selective hydrogenation of 3-nitrostyrene,Co-NAC catalyst affords high catalytic productivity(19.7 h-1),which is superior to the cobalt nanoparticles(NPs)catalysts and most of the recently reported Co-based catalysts.This is attributed to the highly accessible atomically-dispersed Co active sites,the high surface area with ordered-mesoporous morphology and the prominent high content of nitrogen dopants.Notably,Co-NAC catalyst displays resistance towards sulfur-containing poisons(20 equivalents)and strong non-oxidizing acid(8 M),showing great potential for continuous application in the chemical industry. | Yuemin Lin Renfeng Nie Yuting Li Xun Wu Jiaqi Yu Shaohua Xie Yajing Shen Shanjun Mao Yuzhuo Chen Dan Lu Zongbi Bao Qiwei Yang Qilong Ren Yiwen Yang Fudong Liu Long Qi Wenyu Huang Zhiguo Zhang | 2022 | Nano Research2022，15，12： | 0 |
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