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| 1 | CRISPR/Cas9-mediated PINK1 deletion leads to neurodegeneration in rhesus monkeys显示文摘Dear Editor,PINK1 mutations cause autosomal recessive and early-onset Parkinson’s disease(PD)with selective neurodegeneration.Unfortunately,current PINK1 knockout(KO)mouse1,2 and pig models3,4 are unable to recapitulate the selective and overt neurodegeneration seen in PD patients.Furthermore,endogenous Pink1 in the mouse brain is expressed at very low levels and can only be detected via immunoprecipitation,5 meaning that PINK1’s function in the mammalian brain needs to be assessed using larger animals that are closer to humans.We previously used CRISPR/Cas9 to target the monkey gene in one-cell stage embryos.6 Using the same approach,we designed two gRNAs to target exon 2(T1)and exon 4(T2),which encode a kinase domain in the PINK1 gene of rhesus monkeys(Fig.1a).CRISPR/Cas9 and PINK1 gRNAs were injected into one-cell stage rhesus monkey embryos.A T7E1 assay and sequencing of PCR products from the injected embryos showed high efficiency(61.5%)in targeting PINK1(Fig.1b and Supplementary information,Fig.S1).Transfer of 87 embryos to 28 surrogate rhesus monkeys resulted in 11 pregnancies(39.2%)(Fig.1b).Eleven fetuses developed to term and were born naturally.Of these live monkeys,eight carried PINK1 mutations(M),and three were wild type(WT).However,three mutant monkeys(M1,M3 and M4)were newborn triplets that struggled to survive and died 3-4 days after birth.One WT newborn monkey also died after a difficult labor.Another mutant monkey(M2)died 7 days after birth without noticeable warning signs or symptoms.The other three mutant monkeys(M6,M7 and M8)have lived for three years;M5,however,reduced its food intake and showed weakness at the age of 1.5 years,and died 30 days after anesthesia for MRI examination. | Weili Yang Yunbo Liu Zhuchi Tu Chong Xiao Sen Yan Xishan Ma Xiangyu Guo Xiusheng Chen Peng Yin Zhengyi Yang Su Yang Tianzi Jiang Shihua Li Chuan Qin Xiao-Jiang Li | 2019 | Cell Research2019，29，4： | 12 |
| 2 | A double-blind,randomized,placebo-and positive-controlled phase III trial of 1% benvitimod cream in mild-to-moderate plaque psoriasis显示文摘Background:Benvitimod cream,a novel synthetic small molecule,was effective in treating mild-to-moderate plaque psoriasis.We conducted a phase III clinical trial to assess the efficacy and safety of benvitimod cream in patients with mild-to-moderate plaque psoriasis.Methods:We randomly assigned 686 patients(2:1:1)to receive 1%benvitimod cream,0.005%calcipotriol ointment or placebo twice a day for 12 weeks.The primary efficacy end points were the percentage of patients with a 75%or greater reduction from baseline in the psoriasis area and severity index(PASI 75)score and with a score of 0 or 1 in static physician’s global assessment(sPGA)at week 12.Results:The results showed that 50.4%of patients in the benvitimod group achieved PASI 75,which was significantly higher than that in the calcipotriol(38.5%,P<0.05)and placebo(13.9%,P<0.05)groups.The proportion of patients achieving an sPGA score 0 or 1 was 66.3%in the benvitimod group and 63.9%in the calcipotriol group,which were both significantly higher than that in the placebo group(34%,P<0.05).In the long-term follow-up study,50.8%of patients experienced recurrence.After retreatment with 1%benvitimod,73.3%of patients achieved an sPGA score of 0 or 1 again at week 52.Adverse events included application site irritation,follicular papules,and contact dermatitis.No systemic adverse reactions were reported.Conclusion:During this 12-week study,benvitimod cream was demonstrated with high effectiveness and safety in patients with mild-to-moderate plaque psoriasis. | Lin Cai Gen-Hui Chen Qian-Jin Lu Min Zheng Yu-Zhen Li Jin Chen Jie Zheng Fu-Ren Zhang Jian-Bin Yu Sen Yang Fu-Qiu Li Sheng-Xiang Xiao Qiu-Ning Sun Jin-Hua Xu Xing-Hua Gao Hong Fang Tian-Wen Gao Fei Hao Quan-Zhong Liu Ya-Ting Tu Ruo-Yu Li Bao-Xi Wang Dan-Qi Deng Qing-Shan Zheng Hong-Xia Liu Jian-Zhong Zhang | 2020 | Chinese Medical Journal2020，，24： | 11 |
| 3 | Hepatitis C virus RNA detection in serum and peripheral blood mononuclear cells of patients with hepatitis C显示文摘HepatitisCvirusRNAdetectioninserumandperipheralbloodmononuclearcelsofpatientswithhepatitisCZHOUPing,CAIQing,CHENYouChun,ZHA... | ZHOU Ping, CAI Qing, CHEN You Chun, ZHANG Mu Sen, GUAN Jian and LI Xiao Juan | 1997 | World Journal of Gastroenterology1997，3，2： | 10 |
| 4 | Energy-efficient adaptive sensor scheduling for target tracking in wireless sensor networks显示文摘Sensor scheduling is essential to collaborative target tracking in wireless sensor networks(WSNs).In the existing works for target tracking in WSNs,such as the information-driven sensor query(IDSQ),the tasking sensors are scheduled to maximize the information gain while minimizing the resource cost based on the uniform sampling intervals,ignoring the changing of the target dynamics and the specific desirable tracking goals.This paper proposes a novel energyefficient adaptive sensor scheduling approach that jointly selects tasking sensors and determines their associated sampling intervals according to the predicted tracking accuracy and tracking energy cost.At each time step,the sensors are scheduled in alternative tracking mode,namely,the fast tracking mode with smallest sampling interval or the tracking maintenance mode with larger sampling interval,according to a specified tracking error threshold.The approach employs an extended Kalman filter(EKF)-based estimation technique to predict the tracking accuracy and adopts an energy consumption model to predict the energy cost.Simulation results demonstrate that,compared to a non-adaptive sensor scheduling approach,the proposed approach can save energy cost significantly without degrading the tracking accuracy. | Wendong XIAO Sen ZHANG Jianyong LIN Chen Khong THAM | 2010 | 控制理论与应用(英文版)2010，8，1： | 9 |
| 5 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 6 | Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency显示文摘Objective To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease(KBD) and in an established T-2 toxin-and selenium(Se) deficiency-induced rat model. Methods Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase d UTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and m RNA levels were determined by real-time reverse transcription polymerase chain reaction. Results Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and m RNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet. Conclusion T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD. | YANG Hao Jie ZHANG Ying WANG Zhi Lun XUE Sen Hai LI Si Yuan ZHOU Xiao Rong ZHANG Meng FANG Qian WANG Wen Jun CHEN Chen DENG Xiang Hua CHEN Jing Hong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 7 |
| 7 | Scalable fabrication of printed Zn//MnO2 planar micro-batteries with high volumetric energy density and exceptional safety显示文摘The rapid development of printed and microscale electronics imminently requires compatible micro-batteries(MBs)with high performance,applicable scalability,and exceptional safety,but faces great challenges from the ever-reported stacked geometry.Herein the first printed planar prototype of aqueous-based,high-safety Zn//MnO2 MBs,with outstanding performance,aesthetic diversity,flexibility and modularization,is demonstrated,based on interdigital patterns of Zn ink as anode and MnO2 ink as cathode,with high-conducting graphene ink as a metal-free current collector,fabricated by an industrially scalable screen-printing technique.The planar separator-free Zn//MnO2 MBs,tested in neutral aqueous electrolyte,deliver a high volumetric capacity of 19.3 mAh/cm^3(corresponding to 393 mAh/g)at 7.5 mA/cm^3,and notable volumetric energy density of 17.3 mWh/cm^3,outperforming lithium thin-film batteries(≤10 mWh/cm^3).Furthermore,our Zn//MnO2 MBs present long-term cyclability having a high capacity retention of 83.9%after 1300 cycles at 5 C,which is superior to stacked Zn//MnO2 batteries previously reported.Also,Zn//MnO2 planar MBs exhibit exceptional flexibility without observable capacity decay under serious deformation,and remarkably serial and parallel integration of constructing bipolar cells with high voltage and capacity output.Therefore,low-cost,environmentally benign Zn//MnO2 MBs with in-plane geometry possess huge potential as high-energy,safe,scalable and flexible microscale power sources for direction integration with printed electronics. | Xiao Wang Shuanghao Zheng Feng Zhou Jieqiong Qin Xiaoyu Shi Sen Wang Chenglin Sun Xinhe Bao Zhong-Shuai Wu | 2020 | National Science Review2020，7，1： | 5 |
| 8 | A novel synthesis of pyrazolo[3,4-b]pyridine derivatives through multi-component reaction in ionic liquid显示文摘An efficient and novel procedure for the preparation of pyrazolo[3,4-b]pyridine derivatives through multi-component reaction of aldehyde, 5-amino-3-methyl-1-phenylpyrazole and malononitrile or cyanoacetate in [bmim][BF4] is described in this paper. Advantages of the method presented here include mild conditions, high yields together with a green nature and ease of recovery and reuse of the reaction medium. | Xin Ying Zhang Xiao Yan Li Xue Sen Fan Xia Wang Jian Ji Wang Gui Rong Qu | 2008 | Chinese Chemical Letters2008，19，2： | 3 |
| 9 | Scalable and fast fabrication of graphene integrated micro-supercapacitors with remarkable volumetric capacitance and flexibility through continuous centrifugal coating显示文摘Microscale electrochemical energy storage devices,e.g., micro-supercapacitors(MSCs),possessing tailored performance and diversified form factors of lightweight,miniaturization,flexibility and exceptional integration are highly necessary for the smart power sources-unitized electronics.Despite the great progress,the fabrication of MSCs combining high integration with high volumetric performance remains largely unsolved.Herein,we develop a simple,fast and scalable strategy to fabricate graphene based highly integrated MSCs by a new effective continuous centrifugal coating technique.Notably,the resulting highly conductive graphene films can act as not only patterned microelectrodes but also metal-free current collectors and interconnects,endowing modular MSCs with high integrity,remarkable flexibility,tailored voltage and capacitance output,and outstanding performance uniformity.More importantly,the strong centrifugal force and shear force generated in continuous centrifugal coating process lead to graphene films with high alignment,compactness and packing density,contributing to excellent volumetric capacitance of ~31.8 F cm^(-3) and volumetric energy density of ~2.8 mWh cm^(-3),exceeding most reported integrated MSCs.Therefore,our work paves a novel way for simple and scalable fabrication of integrated MSCs and offers promising opportunities as standalone microscale power sources for new-generation electronics. | Xiaoyu Shi Lijun Tian Sen Wang Pengchao Wen Ming Su Han Xiao Pratteek Das Feng Zhou Zhaoping Liu Chenglin Sun Zhong-Shuai Wu Xinhe Bao | 2021 | Journal of Energy Chemistry2021，30，1： | 3 |
| 10 | Benzimidazoles induce concurrent apoptosis and pyroptosis of human glioblastoma cells via arresting cell cycle显示文摘Glioblastoma multiforme(GBM)is the most malignant and lethal primary brain tumor in adults accounting for about 50%of all gliomas.The only treatment available for GBM is the drug temozolomide,which unfortunately has frequent drug resistance issue.By analyzing the hub genes of GBM via weighted gene co-expression network analysis(WGCNA)of the cancer genome atlas(TCGA)dataset,and using the connectivity map(CMAP)platform for drug repurposing,we found that multiple azole compounds had potential anti-GBM activity.When their anti-GBM activity was examined,however,only three benzimidazole compounds,i.e.flubendazole,mebendazole and fenbendazole,potently and dose-dependently inhibited proliferation of U87 and U251 cells with IC50 values below 0.26μM.Benzimidazoles(0.125−0.5μM)dose-dependently suppressed DNA synthesis,cell migration and invasion,and regulated the expression of key epithelial-mesenchymal transition(EMT)markers in U87 and U251 cells.Benzimidazoles treatment also dose-dependently induced the GBM cell cycle arrest at the G2/M phase via the P53/P21/cyclin B1 pathway.Furthermore,the drugs triggered pyroptosis of GBM cells through the NF-κB/NLRP3/GSDMD pathway,and might also concurrently induced mitochondria-dependent apoptosis.In a nude mouse U87 cell xenograft model,administration of flubendazole(12.5,25,and 50 mg·kg^(−1)·d^(−1),i.p,for 3 weeks)dose-dependently suppressed the tumor growth without obvious adverse effects.Taken together,our results demonstrated that benzimidazoles might be promising candidates for the treatment of GBM. | Li-wen Ren Wan Li Xiang-jin Zheng Jin-yi Liu Yi-hui Yang Sha Li Sen Zhang Wei-qi Fu Bin Xiao Jin-hua Wang Guan-hua Du | 2022 | Acta Pharmacologica Sinica2022，43，1： | 3 |
| 11 | Heavy soil drying during mid-to-late grain filling stage of the main crop to reduce yield loss of the ratoon crop in a mechanized rice ratooning system显示文摘Yield loss(Y_(Loss)) in the ratoon crop due to crushing damage to left stubble from mechanical harvesting of the main crop is a constraint for wide adoption of mechanized rice ratooning technology.Soil drying before the harvest of the main crop has been proposed to overcome this problem.The objective of this study was to determine the effect of soil drying during the mid-to-late grain filling stage of the main crop on grain yield of the ratoon crop in a mechanized rice ratooning system.Field experiments were conducted to compare Y_(Loss) between light(LD) and heavy(HD) soil drying treatments in Hubei province,central China in 2017 and 2018.Y_(Loss) was calculated as the percentage of yield reduction in the ratoon crop with the main crop harvested mechanically,relative to the grain yield of the ratoon crop with the main crop harvested manually.In comparison with LD,soil hardness was increased by 42.8%-84.7% in HD at the 5-20 cm soil depth at maturity of the main crop.Soil hardness at 5 and 10 cm depths reached respectively 4.05 and 7.07 kg cm^(-2) in HD.Soil drying treatment did not significantly affect the grain yield of the main crop.Under mechanical harvesting of the main crop,HD increased the grain yield of the ratoon crop by 9.4% relative to LD.Consequently,Y_(Loss) was only 3.4% in HD,in contrast to 16.3% in LD.The differences in grain yield and Y_(Loos) between the two soil drying treatments were explained mainly by panicles m^(-2),which was increased significantly by HD in the track zone of the ratoon crop compared with LD.These results suggest that heavy soil drying practice during the mid-to-late grain filling stage of the main crop is effective for reducing Y_(Loss) of the ratoon crop in a mechanized rice ratooning system. | Chang Zheng Yuechao Wang Shen Yuan Sen Xiao Yating Sun Jianliang Huang Shaobing Peng | 2022 | The Crop Journal2022，10，1： | 3 |
| 12 | Current Development Situation and Prospects of Blueberry in Guangdong Province显示文摘With high nutrition and health value,blueberry receives much concern in recent years. Blueberry experts in the world are cultivating new blueberry varieties. They have come up with constructive theoretical support and planting technology researches and are constantly devoted to expanding blueberry planting latitudes. This paper elaborated researches of blueberry at home and abroad,current development situation of blueberry in China and Guangdong Province,analyzed strengths of planting blueberry in Guangdong. Besides,it came up with recommendations for development of blueberry industry. Finally,it discussed development prospects of blueberry planting in Guangdong. | Lixia GAO Hualan XIAO Sen LI Fengmin LIU Aiqiong MO | 2015 | Asian Agricultural Research2015，7，9： | 2 |
| 13 | The MING proposal at SHINE:megahertz cavity enhanced X‑ray generation显示文摘The cavity-based X-ray free-electron laser(XFEL)has promise in producing fully coherent pulses with a bandwidth of a few meV and very stable intensity,whereas the currently existing self-amplified spontaneous emission(SASE)XFEL is capable of generating ultra-short pulses with chaotic spectra.In general,a cavity-based XFEL can provide a spectral brightness three orders of magnitude higher than that of the SASE mode,thereby opening a new door for cutting-edge scientific research.With the development of superconducting MHz repetition-rate XFEL facilities such as FLASH,European-XFEL,LCLS-II,and SHINE,practical cavity-based XFEL operations are becoming increasingly achievable.In this study,megahertz cavity enhanced X-ray generation(MING)is proposed based on China’s first hard XFEL facility-SHINE,which we refer to as MING@SHINE. | Nan‑Shun Huang Zi‑Peng Liu Bang‑Jie Deng Zi‑Han Zhu Shao‑Hua Li Tao Liu Zheng Qi Jia‑Wei Yan Wei Zhang Sheng‑Wang Xiang Yang‑Yang Lei Ya Zhu Yong‑Zhou He Qi‑Bing Yuan Fei Gao Rong‑Bing Deng Sen Sun Zhi‑Di Lei Zhi‑Qiang Jiang Meng‑Qi Duan Yuan Zhuan Xue‑Fang Huang Peng‑Cheng Dong Zhong‑Liang Li Shang‑Yu Si Lian Xue Si Chen Yong‑Fang Liu Ya‑Jun Tong Hai‑Xiao Deng Zhen‑Tang Zhao | 2023 | Nuclear Science and Techniques2023，34，1： | 2 |
| 14 | Room-temperature fast assembly of 3D macroscopically porous graphene frameworks for binder-free compact supercapacitors with high gravimetric and volumetric capacitances显示文摘Synthesis and applications of three-dimensional(3 D)porous graphene frameworks(GFs)have attracted extensive interest owing to their intriguing advantages of high specific surface area,enriched porosity,excellent electrical conductivity,exceptional compressibility and processability.However,it is still challenging for economically viable,fast and scalable assembly of 3 D GFs at room-temperature.Herein,we reported a one-step scalable strategy for fast self-assembly of graphene oxide into 3 D macroscopically porous GFs,with assistance of polyoxometalates(POM)as functional cross-linker and hydrazine hydrate as reductant at room-temperature.The resulting 3 D interconnected macroporous POM-GFs uniformly decorated with ultrasmall POM nanoclusters were directly processed into binder-/additive-free film compact electrodes(1.68 g cm^(-3))with highly aligned,layer-stacked structure and electrically conductivity(622 S m-1)for high-performance supercapacitors,showing an impressive gravimetric capacitance of205 F g-1,volumetric capacitance of 334 F cm^(-3) at 1 mV s^(-1),and remarkable cycling stability with capacitance retention of 83%after 10,000 cycles,outperforming the most reported GFs.Further,the solid-state supercapacitors offered excellent gravimetric capacitance of 157 F g-1 exceptionally volumetric capacitance of 115 F cm^(-3) at 2 mV s^(-1) based on single electrode,and volumetric energy density of2.6 mWh cm^(-3).Therefore,this work will open novel opportunities to room-temperature fast assembly of 3 D porous graphene architectures for high-energy-density supercapacitors. | Sen Wang Xiao Wang Chenglin Sun Zhong-Shuai Wu | 2021 | Journal of Energy Chemistry2021，30，10： | 2 |
| 15 | High-payload completely reversible data hiding in encrypted images by an interpolation technique显示文摘本文提出一种新型的高容量密文图像可逆信息隐藏方法。与直接在密文图像中嵌入数据不同,内容拥有者首先利用插值技术在加密前估算出嵌入的位置并生成地图;其次,数据隐藏者根据位置地图,通过翻转密文图像的最高有效位来嵌入额外的数据。在接收端口,接收者在提取嵌入数据和重构图像前先解密图像。实验结果表明该方法可实现完全可逆,即数据提取和图像恢复都无误。此外,与现有大多数密文图像可逆隐藏方案相比,本方案可实现更高的嵌入容量。 | Di XIAO Ying WANG Tao XIANG Sen BAI | 2017 | Frontiers of Information Technology & Electronic Engineering2017，18，11： | 2 |
| 16 | Immunogenicity and safety of NVSI-06-07 as a heterologous booster after priming with BBIBP-CorV:a phase 2 trial显示文摘The increased coronavirus disease 2019(COVID-19)breakthrough cases pose the need of booster vaccination.We conducted a randomised,double-blinded,controlled,phase 2 trial to assess the immunogenicity and safety of the heterologous prime-boost vaccination with an inactivated COVID-19 vaccine(BBIBP-CorV)followed by a recombinant protein-based vaccine(NVSI-06-07),using homologous boost with BBIBP-CorV as control.Three groups of healthy adults(600 individuals per group)who had completed two-dose BBIBP-CorV vaccinations 1–3 months,4–6 months and≥6 months earlier,respectively,were randomly assigned in a 1:1 ratio to receive either NVSI-06-07 or BBIBP-CorV boost.Immunogenicity assays showed that in NVSI-06-07 groups,neutralizing antibody geometric mean titers(GMTs)against the prototype SARS-CoV-2 increased by 21.01–63.85 folds on day 28 after vaccination,whereas only 4.20–16.78 folds of increases were observed in control groups.For Omicron variant,the neutralizing antibody GMT elicited by homologous boost was 37.91 on day 14,however,a significantly higher neutralizing GMT of 292.53 was induced by heterologous booster.Similar results were obtained for other SARS-CoV-2 variants of concerns(VOCs),including Alpha,Beta and Delta.Both heterologous and homologous boosters have a good safety profile.Local and systemic adverse reactions were absent,mild or moderate in most participants,and the overall safety was quite similar between two booster schemes.Our findings indicated that NVSI-06-07 is safe and immunogenic as a heterologous booster in BBIBP-CorV recipients and was immunogenically superior to the homologous booster against not only SARS-CoV-2 prototype strain but also VOCs,including Omicron. | Nawal Al Kaabi Yun Kai Yang Jing Zhang Ke Xu Yu Liang Yun Kang Ji Guo Su Tian Yang Salah Hussein Mohamed Saif ElDein Shuai Shao Sen Sen Yang Wenwen Lei Xue Jun Gao Zhiwei Jiang Hui Wang Meng Li Hanadi Mekki Mekki Walid Zaher Sally Mahmoud Xue Zhang Chang Qu Dan Ying Liu Jing Zhang Mengjie Yang Islam Eltantawy Peng Xiao Zhao Nian Wang Jin Liang Yin Xiao Yan Mao Jin Zhang Ning Liu Fu Jie Shen Liang Qu Yun Tao Zhang Xiao Ming Yang Guizhen Wu Qi Ming Li | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 2 |
| 17 | Connexin 43 Reverses Malignant Phenotypes of Glioma Stem Cells by Modulating E‐Cadherin显示文摘 | Shi‐CangYu Hua‐LiangXiao Xue‐FengJiang Qing‐LiangWang YanLi Xiao‐JunYang Yi‐FangPing Jiang JieDuan Jian‐YongJiang Xian‐ZongYe Sen‐LinXu Yang‐HongXin Xiao‐HongYao Jian‐HongChen Wei‐HuaChu WeiSun BingWang Ji MingWang XiaZhang Xiu‐WuBian | 2012 | STEM CELLS2012，，2： | 1 |
| 18 | Repair of Segmental Long Bone Defect in a Rabbit Radius Nonunion Model: Comparison of Cylindrical Porous Titanium and Hydroxyapatite Scaffolds显示文摘 | Ming Zhang Guang‐lin Wang Hong‐fang Zhang Xu‐dong Hu Xiao‐yuan Shi Sen Li Wei Lin | 2014 | Artificial Organs2014，，6： | 1 |
| 19 | Glucose - induced phosphorylation tif the insulin receptor functional effects and characterization d phosphorylation sites显示文摘 | Pillay TS Xiao Sen Olefsky JM | | 0，，： | 1 |
| 20 | TBN improves motor function and prolongs survival in SOD1G93A and TDP-43M337V mouse model of amyotrophic lateral sclerosis显示文摘OBJECTIVE Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative dis⁃ease characterized by progressive loss of upper and lower motor neurons that results in skeletal muscle atrophy,weakness and paralysis.Oxida⁃tive stress plays a key role in the pathogenesis of ALS,including familial forms of the disease arising from mutation of the gene coding for superoxide dismutase 1(SOD1).Moreover,although the pathogenesis of ALS is unclear,the abnormal accumulation of TAR DNA-binding pro⁃tein of 43 ku(TDP-43)is a pathological feature that exists in almost all patients.Thus far,there is no drug that can cure ALS/FTLD.Tetramethyl⁃pyrazine nitrone(TBN)is a derivative of tetra⁃methylapyrazine,derived from the traditional Chinese medicine Ligusticum chuanxiong,which has been widely proven to have therapeutic effects on models of various neurodegenerative diseases.TBN is currently under clinical investi⁃gation for several indications including a phaseⅡtrial of ALS.Here,we explored the therapeutic effect of TBN in the SOD1G93A and TDP-43M337V ALS mouse model.METHODS In the SOD1G93A transgenic mouse model,TBN was administered to mice by intraperitoneal or intragastric injection after the onset of motor deficits.At the same time,we unilaterally and bilaterally injected the TDP-43M337V virus into the striatum of the WT mouse,and gave the TBN treatment after the mice developed a phenotype.After administering these two models for a period of time,we con⁃ducted behavioral tests,including rotarod test,balance beam test,climbing pole test,etc,to evaluate the efficacy of TBN on SOD1G93A and TDP-43M337V models.Furthermore,we explored the possible mechanism of action of TBN in the treatment of ALS through Western blotting and immunohistochemistry/immunofluorescence staining analysis.RESULTS In the SOD1G93A transgenic mouse model,TBN slowed the pro⁃gression of motor neuron disease as evidenced by improved motor performance,reduced spinal motor neuron loss and the associated glial response,and decreased skeletal muscle fiber denervation and fibrosis.TBN treatment activated mitochondrial antioxidant activity through the PGC-1α/Nrf2/HO-1 pathway and decreased the expression of human SOD1.What′s more,in the TDP-43M337V mice model,the results showed that in mice with unilateral injection of TDP-43M337V,TBN improved motor deficits and cognitive im⁃pairment in the early stages of disease progres⁃sion.In mice with bilateral injection of TDP-43M337V into the striatum,TBN not only improved motor function but also prolonged survival rate.Moreover,we show that its therapeutic effect may be through activation of the Akt/mTOR/GSK-3βand AMPK/PGC-1α/Nrf2 signaling pathways.In summary,TBN is a promising agent for the treat⁃ment of ALS/FTLD.CONCLUSION TBN has shown good efficacy in both SOD1 and TDP-43 ALS-related models,and it may act by activating the AMPK/PGC-1α/Nrf2 signaling pathway,which shows some light for the development of ALS therapeutic drugs. | HUANG Chun-hui LI Jun ZHANG Gui-liang LIN Ying-qi LI Cai-juan ZHENG Xiao SONG Xi-cheng HAN Bo-feng GUO Bao-jian TU Zhu-chi ZHANG Jun SUN Ye-wei WANG Yu-qiang YAN Sen ZHANG Zai-jun | 2021 | 中国药理学与毒理学杂志2021，35，9： | 1 |
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