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| 1 | Clinicopathological and prognostic analysis of 429 patients with intrahepatic cholangiocarcinoma显示文摘AIM:To understand the clinicopathological characteristics and treatment selections and improve survival and provide valuable information for patients with intrahepatic cholangiocarcinoma(ICC). METHODS:We retrospectively evaluated 5311 liver cancer patients who received resection between October 1999 and December 2003.Of these,429(8.1%)patients were diagnosed with ICC,and their clinicopathological, surgical,and survival characteristics were analyzed. RESULTS:Upper abdominal discomfort or pain(65.0%), no symptoms(12.1%),and hypodynamia(8.2%)were the major causes for medical attention.Laboratory tests showed 198(46.4%)patients were HBsAg positive, 90(21.3%)hadα-fetoprotein>20μg/L,50(11.9%) carcinoembryonic antigen>10μg/L,and 242(57.5%) carbohydrate antigen 19-9(CA19-9)>37 U/mL.Survival data was available for 329(76.7%)patients and their mean survival time was 12.4 mo.The overall survival of the patients with R0,R1 resection and punching exploration were 18.3,6.6 and 5.6 mo,respectively. Additionally,CA19-9>37 U/mL was associated with lymph node metastases,but inversely associated withcirrhosis.Multivariate analysis indicated that radical resection,lymph node metastases,macroscopic tumor thrombi and size,and CA19-9 were associated with prognosis. CONCLUSION:Surgical radical resection is still the most effective means to cure ICC.Certain laboratory tests(such as CA19-9)can effectively predict the survival of the patients with ICC. | Wei-Feng Shen Wei Zhong Feng Xu Tong Kan Li Geng Feng Xie Cheng-Jun Sui Jia-Mei Yang | 2009 | World Journal of Gastroenterology2009，15，47： | 39 |
| 2 | Enhancement of acellular cartilage matrix scaffold by Wharton’s jelly mesenchymal stem cell-derived exosomes to promote osteochondral regeneration显示文摘Articular cartilage defect repair is a problem that has long plagued clinicians.Although mesenchymal stem cells(MSCs)have the potential to regenerate articular cartilage,they also have many limitations.Recent studies have found that MSC-derived exosomes(MSC-Exos)play an important role in tissue regeneration.The purpose of this study was to verify whether MSC-Exos can enhance the reparative effect of the acellular cartilage extracellular matrix(ACECM)scaffold and to explore the underlying mechanism.The results of in vitro experiments show that human umbilical cord Wharton’s jelly MSC-Exos(hWJMSC-Exos)can promote the migration and proliferation of bone marrow-derived MSCs(BMSCs)and the proliferation of chondrocytes.We also found that hWJMSC-Exos can promote the polarization of macrophages toward the M2 phenotype.The results of a rabbit knee osteochondral defect repair model confirmed that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration.We demonstrated that hWJMSC-Exos can regulate the microenvironment of the articular cavity using a rat knee joint osteochondral defect model.This effect was mainly manifested in promoting the polarization of macrophages toward the M2 phenotype and inhibiting the inflammatory response,which may be a promoting factor for osteochondral regeneration.In addition,microRNA(miRNA)sequencing confirmed that hWJMSC-Exos contain many miRNAs that can promote the regeneration of hyaline cartilage.We further clarified the role of hWJMSC-Exos in osteochondral regeneration through target gene prediction and pathway enrichment analysis.In summary,this study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. | Shuangpeng Jiang Guangzhao Tian Zhen Yang Xiang Gao Fuxin Wang Juntan Li Zhuang Tian Bo Huang Fu Wei Xinyu Sang Liuqi Shao Jian Zhou Zhenyong Wang Shuyun Liu Xiang Sui Quanyi Guo Weimin Guo Xu Li | 2021 | Bioactive Materials2021，6，9： | 11 |
| 3 | Molecular Cloning and Expression of Squalene Epoxidase from a Medicinal Plant, Bupleurum chinense显示文摘Objective In plant, squalene epoxidase(SE) catalyzes the first oxygenation step in the biosynthetic pathway of triterpenoid and phytosterol, representing one of the rate-limiting enzymes in this pathway. Bupleurum chinense is an important medicinal herb with its major active constituents such as triterpenoid saponins and saikosaponins. In order to obtain the series of enzymatic genes involved in saikosaponin biosynthesis, a c DNA of SE, designated Bc SE1, was cloned from B. chinense. Methods The Bc SE1 gene was cloned by homology-based PCR and 5'/3' RACE methods from the adventitious roots of B. chinense. The physical and chemical parameters of Bc SE1 protein were predicted by protparam. In order to discover hints in amino acid sequences on the dominant functions in the biosynthesis of saponin or phytosterol, sequences of SE from other plants were downloaded from NCBI for sequences alignment and phylogenetic analysis. Bc SE1 was cloned into a yeast mutant KLN1(MATa, erg1::URA3, leu2, ura3, and trp1) to verify the enzyme activity of Bc SE1. Additionally, the tissue-specific expression and methyl jasmonate(Me JA) inducibility of Bc SE1 were investigated using quantitative real-time PCR. Results The predicted protein of Bc SE1 is highly similar to SEs from other plants sharing amino acid sequence identities of up to 88%. The Bc SE1 can functionally complement with yeast SE gene(ERG1) when expressed in the KLN1 mutant(MATa, erg1::URA3, leu2, ura3, and trp1). Using as controls with β-amyrin synthase(β-AS) which is presumed to catalyze the first committed step in saikosaponin biosynthesis and a cycloartenol synthase(CAS) relating to the phytosterol biosynthesis, the transcript of Bc SE1 was significantly elevated by Me JA in adventitious roots of B. chinense and the transcript of Bc SE1 was most abundant in the fruits and flowers of plants, followed by that in the leaves and roots, and least in stems. Conclusion It is the first time to illustrate the molecular information of SE in B. chinense and to clone the full-length SE gene in plants of genus Bupleurum L. | Ke Gao Jie-sen Xu Jing Sun Yan-hong Xu Jian-he Wei Chun Sui | 2016 | Chinese Herbal Medicines2016，8，1： | 10 |
| 4 | Rapid and sensitive detection of pesticides by surface-enhanced Raman spectroscopy technique based on glycidyl methacrylate-ethylene dimethacrylate(GMA-EDMA) porous material显示文摘A novel method of fast and sensitive SERS detection using GMA-EDMA porous material combined with a miniature device was reported in this study.A100μL solution containing sample,silver colloid and NaCl was evenly mixed to ensure the sample molecules would adsorb onto silver nanoparticles.Then the mixture was added onto the porous material surface slowly,so that the aggregation of silver colloid would stay on the surface while the liquid components would flow away.This technology can improve the sensitivity of SERS detection.By this method,two pesticides tricyclazole and paraquat were successfully detected at concentrations of 5×10^(-3) mg/L and 1×10^(-3) mg/L,respectively. | Qing-Qing Li Yi-Ping Du Ying Xu Xuan Wang Shi-Qian Ma Jin-Pei Geng Peng Cao Tao Sui | 2013 | Chinese Chemical Letters2013，24，4： | 10 |
| 5 | Si-and alkali-rich melt inclusions in minerals of mantle peridotites from eastern China:Implication for lithospheric evolution显示文摘Minerals of spinel- and garnet-facies mantle xenoliths entrained in Cenozoic basalts from eastern China (North China, Northeastern China and Southeastern China coastal area) contains lots of melt inclusions. Studies on these melt inclusions show that the glass inclusions are rich in SiO2 (60%―68%) and alkalis (K2O+Na2O=5%―11%, especially for K2O) as well as volatiles such as H2O and CO2 (2%―7%), which belong to dacites and andesites of the high-K calcic alkali series rocks with few shoshonites. High Al and Ca diopside in melt inclusion is the product of melt crystallization at high temperature and pressure, rather than the product of devitrification. Results show that these K-rich (in general K2O>3%) intermediate-acidic silicate melt inclusions have characteristics of continent without a genetical link to host basalts and their phenocrystic minerals. Thus, these trapped melt inclusions represent melts of Mesozoic lithospheric mantle-crust interaction and imply that the continental litho-spheric mantle beneath eastern China had undergone fragmentation and recreation processes during the Mesozoic and Cenozoic periods. This result undoubtly provides important implication for the evo-lution of sub-continental lithosphere beneath eastern China. We propose that these Si- and alka-lis-rich melts should be responsible for the mantle chemical heterogeneity underneath eastern China. | FAN Qicheng1, SUI Jianli2, XU Ping2, LI Ni1, SUN Qian1 & WANG Tuanhua1 1. Institute of Geology, China Seismological Bureau, Beijing 100029, China 2. Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China | 2006 | Science China Earth Sciences2006，49，1： | 9 |
| 6 | Perchlorate removal using granular activated carbon supported iron compounds: Synthesis, characterization and reactivity显示文摘Synthesis and use of the iron compounds supported on granular activated carbon (ICs/GAC) have shown significant environmental implications for perchlorate (ClO 4 ) removal. ICs/GAC was synthesized via hydrolyzing FeSO 4 ·7H 2 O on GAC, reduced by NaBH 4 solution in polyethylene glycol 6000 and ethanol solution, dried in vacuum condition and exposed to air. Synthesized ICs/GAC was characterized using transmission electron micrograph (TEM), Brunauer-Emmett-Teller, X-ray photoelectron spectroscopy (XPS). ICs/GAC was determined to be containing a large amount of FeOHSO 4 , Fe 2 O 3 and a small amount of zero-valent iron (ZVI) nanoparticles according to TEM and XPS measurements. Batch static kinetic tests showed that 97% of ClO 4 was removed within 10 hr at 90°C and 86% of ClO 4 was removed within 12 hr at 25°C, at ICs/GAC dosage of 20 g/L. The experimental results also showed that FeOHSO 4 and Fe 2 O 3 nanoparticles have the function of perchlorate adsorption and play important roles in ClO 4 removal. The activation energy (E a ) was determined to be 9.56 kJ/mol. | Jianhong Xu Naiyun Gao Yulin Tang Yang Deng Minhao Sui | 2010 | Journal of Environmental Sciences2010，22，11： | 8 |
| 7 | Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation显示文摘AIM: To investigate the effect of different dietary fatty acids on hepatic inflammasome activation.METHODS: Wild-type C57BL/6 mice were fed either a high-fat diet or polyunsaturated fatty acid(PUFA)-enriched diet. Primary hepatocytes were treated with either saturated fatty acids(SFAs) or PUFAs as well as combined with lipopolysaccharide(LPS). The expression of NOD-like receptor protein 3(NLRP3) inflammasome, peroxisome proliferator-activated receptor-γ and nuclear factor-kappa B(NF-κB) was determined by real-time PCR and Western blot. The activity of Caspase-1 and interleukine-1β production were measured.RESULTS: high-fat diet-induced hepatic steatosis was sufficient to induce and activate hepatic NLRP3 inflammasome. SFA palmitic acid(PA) directly activated NLRP3 inflammasome and increased sensitization to LPS-induced inflammasome activation in hepatocytes. In contrast, PUFA docosahexaenoic acid(Dh A) had thepotential to inhibit NLRP3 inflammasome expression in hepatocytes and partly abolished LPS-induced NLRP3 inflammasome activation. Furthermore, a highfat diet increased but PUFA-enriched diet decreased sensitization to LPS-induced hepatic NLRP3 inflammasome activation in vivo. Moreover, PA increased but Dh A decreased phosphorylated NF-κB p65 protein expression in hepatocytes.CONCLUSION:Hepatic NLRP 3 inflammasome activation played an important role in the development of non-alcoholic fatty liver disease. Dietary SFAs and PUFAs oppositely regulated the activity of NLRP3 inflammasome through direct activation or inhibition of NF-κB. | Yong-heng Sui Wen-jing Luo Qin-Yu Xu jing hua | 2016 | World Journal of Gastroenterology2016，22，8： | 8 |
| 8 | A study on pathogenicity of hepatitis G virus显示文摘AIM To study the pathogenicity of hepatitis G virus (HGV) and observe the genesis and pathological process of hepatitis G.METHODS HGV-RNA in serum was detected by RT-PCR assay. The immunohistochemical assays of liver tissue were performed with HGV monocoloned antibody (McAb)expressed from the region of HGV NS5 nucleic acid sequence. The clinical and pathological data of 52 patients with hepatitis G were discussed. In animal experiment,the Chinese Rhesus monkeys were infected with the serum of a patient with HGV infection. And the dynamic changes in serology and liver histology of animals were observed.RESULTS One hundred and fifty-four patients with HGVRNA positive were selected from 1552 patients with various kinds of hepatitis. Of 154 patients with HGV infection, 52 were infected with HGV only, which accounted for 33.8% (52/154) and 102 with positive HGVRNA were super-infected with other hepatitis viruses,which accounted for 66.2% (102/154). The clinical and pathological observation showed that the acute and chronic hepatitis could be induced by HGV. The slight abnormality of transaminases ALT and AST in serum of monkeys lasted nearly 12 months and histological results showed a series of pathological changes.CONCLUSION HGV is a hepatotropic virus and has pathogenicty. | Jia-Zhang Xu Zhi-Guo Yang Mei-Zhao Le Mao-Rong Wang Chang-Lun He Yun-Hua Sui Institute of Hepatology,the 81st Hospital of PLA,Nanjing 210002,Jiangsu Province,ChinaDepartment of Pathology,the 81st Hospital of PLA,Nanjing 210002,Jiangsu Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 8 |
| 9 | Application of CRISPR/Cas9 in plant biology显示文摘The CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPRassociated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. | Xuan Liu Surui Wu Jiao Xu Chun Sui Jianhe Wei | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 8 |
| 10 | Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis显示文摘Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer types.However,the effect and molecular mechanism of curcumenol in lung cancer are largely unknown.Here,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer cells.Next,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first time.Subsequently,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated controls.Overexpression of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol treatment.Mechanistically,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of ferroptosis.In conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell death.Therefore,our findings will hopefully provide a valuable drug for treating lung cancer patients. | Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang Qianru Zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui | 2022 | Bioactive Materials2022，7，7： | 8 |
| 11 | Comparative analysis between endometrial proteomes of pregnant and non-pregnant ewes during the peri-implantation period显示文摘Background:Early pregnancy failure has a profound impact on both human reproductive health and animal production.2/3 pregnancy failures occur during the peri-implantation period;however,the underlying mechanism(s)remains unclear.Well-organized modification of the endometrium to a receptive state is critical to establish pregnancy.Aberrant endometrial modification during implantation is thought to be largely responsible for early pregnancy loss.Result:In this study,using well-managed recipient ewes that received embryo transfer as model,we compared the endometrial proteome between pregnant and non-pregnant ewes during implantation period.After embryo transfer,recipients were assigned as pregnant or non-pregnant ewes according to the presence or absence of an elongated conceptus at Day 17 of pregnancy.By comparing the endometrial proteomic profiles between pregnant and non-pregnant ewes,we identified 94 and 257 differentially expressed proteins(DEPs) in the endometrial caruncular and intercaruncular areas,respectively.Functional analysis showed that the DEPs were mainly associated with immune response,nutrient transport and utilization,as well as proteasome-mediated proteolysis.Conclusion:These analysis imply that dysfunction of these biological processes or pathways of DEP in the endometrium is highly associated with early pregnancy loss.In addition,many proteins that are essential for the establishment of pregnancy showed dysregulation in the endometrium of non-pregnant ewes.These proteins,as potential candidates,may contribute to early pregnancy loss. | Haichao Zhao Linlin Sui Kai Miao Lei An Dong Wang Zhuocheng Hou Rui Wang Min Guo Zhilong Wang Jiqiang Xu Zhonghong Wu Jianhui Tian | 2015 | Journal of Animal Science and Biotechnology2015，6，3： | 8 |
| 12 | Highly efficient PdCu3 nanocatalysts for Suzuki-Miyaura reaction显示文摘Suzuki-Miyaura 反应,包含碳卤素的激活结合,特别 C-Cl 契约,因为他们的巨大的工业潜力,引起了普遍注意。然而,这些反应依赖于高度活跃、稳定的催化剂的发展。此处,我们开发了一个方便一个壶综合的湿线路 Pd x Cu y 为 Suzuki-Miyaura 反应的二金属的 nanocrystals。由介绍 Cu,一个地球丰富的元素,当要求的 Pd 的数量被减少时,催化活动极大地被提高。Pd x 不同作文的 Cu y nanocrystals,包括 Pd 3 Cu, Pd 2 Cu, PdCu, PdCu 2, 和 PdCu 3, 被调节 Pd 成功地综合: Cu 比率。他们在在 phenylboronic 酸和 halobenzenes (iodo- , bromo- ,或 chlorobenzene ) 之间的 Suzuki-Miyaura 反应的催化表演证明 PdCu 3 nanocatalyst 表明了最好的功效。 | Weiwei Shi Xiaoqing Chen Suying Xu Jiabin Cui LeyuWang | 2016 | Nano Research2016，9，10： | 7 |
| 13 | SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target显示文摘Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death.However,the origin of excessive damages caused by SARS-CoV-2 remains largely unknown.Here we show that the SARS-CoV-2 envelope(2-E)protein alone is able to cause acute respiratory distress syndrome(ARDS)-like damages in vitro and in vivo. | Bingqing Xia Xurui Shen Yang He Xiaoyan Pan Feng-Liang Liu Yi Wang Feipu Yang Sui Fang Yan Wu Zilei Duan Xiaoli Zuo Zhuqing Xie Xiangrui Jiang Ling Xu Hao Chi Shuangqu Li Qian Meng Hu Zhou Yubo Zhou Xi Cheng Xiaoming Xin Lin Jin Hai-Lin Zhang Dan-Dan Yu Ming-Hua Li Xiao-Li Feng Jiekai Chen Hualiang Jiang Gengfu Xiao Yong-Tang Zheng Lei-Ke Zhang Jingshan Shen Jia Li Zhaobing Gao | 2021 | Cell Research2021，31，8： | 7 |
| 14 | Smart CU1.75S nanocapsules with high and stable photothermal efficiency for NIR photo-triggered drug release显示文摘Thermosensitive 药交货系统(DDS ) 面对主要挑战,例如遥远、可重复的控制在 vivo 温度,尽管这些能增加药的治疗学的功效。处理这个问题,我们涂在红外线附近(NIR ) photothermal Cu 1.75 有 pH/thermos-sensitive 聚合物由的 S nanocrystals 在 situ 聚合。装载内容的 doxorubicine (纪录影片) 直到 40 wt.% ,与纪录影片在正常生理的条件下面正在被漏的不到 8.2 wt.%(pH = 7.4, 37 吗? | Sheng Huang Jing Liu Qian He Hongli Chen Jiabin Cui Suying Xu Yuliang Zhao Chunying Chen Leyu Wang | 2015 | Nano Research2015，8，12： | 7 |
| 15 | Effects of flooding stress on the photosynthetic apparatus of leaves of two Physocarpus cultivars显示文摘We applied under pot-culture conditions and the double-casing pot method to study the characteristics of photosynthetic gas exchange and chlorophyll fluorescence in the leaves of Physocarpus amurensis Maxim(PA) and Physocarpus opulifolius under flooding stress.Our results indicate a significantly higher flooding tolerance of P.opulifolius compared to P.amurensis.Especially in P.amurensis, the limitation of non-stomatal factors played a major role in the advanced stages of flooding stress,observed as a rapid increase of the intercellular CO_2 concentration(C_i) and a decrease of the stomatal limitation value(L_s).The maximal PSII photochemical efficiencies(F_v/F_m) and actual photochemical efficiency(?_(PSII)) in the leaves of P.opulifolius were significantly higher, and the extent of decrease during the flooding process was smaller than in P.amurensis.In addition, the non-chemical quenching(NPQ) in the leaves of P.opulifolius significantly increased from the 10 th day under flooding stress,while the variation of NPQ in the leaves of P.amurensis was much smaller.This indicates that the leaves of P.opulifolius had not only higher PSII photochemical activity, but also improved tolerance to flooding stress, which may be caused by its ability to dissipate excess excitation energy by starting NPQ.At the 16 th day under flooding stress, the PIABSsignificantly decreased with greater extent of decrease than F_v/F_m in the leaves of both Physocarpus,but the decreasing extent of PIABSin P.opulifolius was significantly smaller than in P.amurensis.In the 16 th day under flooding stress, the fluorescence at J and I point(V_J and V_I) in P.amurensis were significantly higher, and the extent of increase in V_J was greater than V_I.However, the variations of V_Jand V_I in the leaves of P.opulifolius were smaller, suggesting that the damage sites of flooding stress to PSII in the leaves of P.amurensis were mainly located in the electron transport process from Q_A at the PSII receptor side to Q_B.Flooding stress reduced the proportion(φ Eo) of luminous energy absorbed by the PSII reaction center for the electron transport following Q_A^-, while the maximum quantum yield(φ Do) of non-photochemical quenching increased.However, the TR_o/RC and ETo/RC in the leaves of P.amurensis decreased accompanied by a dramatic increase of energy(DI_o/RC) from the dissipation in the reaction center.This further indicated that the function of the PSII reaction center in the leaves of P.amurensis was significantly lower than in P.opulifolius. | Huihui Zhang Peng Feng Wei Yang Xin Sui Xin Li Wei Li Rongtao Zhang Siyu Gu Nan Xu | 2018 | Journal of Forestry Research2018，29，4： | 6 |
| 16 | Degradation of carbon tetrachloride in thermally activated persulfate system in the presence of formic acid显示文摘碳四氯化物(CT ) 的热地激活的 persulfate (PS ) 降级面对蚁的酸(FA ) 被调查。结果显示那 CT 降级跟随了零份订单运动模型,和公司 2- | Minhui XU Xiaogang GU Shuguang LU Zhouwei MIAO Xueke ZANG Xiaoliang WU Zhaofu QIU Qian SUI | 2016 | Frontiers of Environmental Science & Engineering2016，10，3： | 6 |
| 17 | Cloning and Expression Analysis on 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase from Aquilaria sinensis显示文摘Objective To clone the full-length cDNA of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGR)from Aquilaria sinensis(AsHMGR1)and to analyze its expression profile in different tissues and in response to different treatments.HMGR is the first rate-limiting enzyme for sesquiterpene synthesis in the mevalonate pathway.Methods RT-PCR and RACE were used to clone the full-length cDNA of HMGR from A.sinensis based on the conserved HMGR gene fragments.The bioinformatic analysis was performed on its nucleic acid and protein sequence.The expression profile of AsHMGR1 in different tissues and in response to different treatments was analyzed by quantitative RT-PCR.Results The full-length AsHMGR1 cDNA was 2026 bp,containing a 1719 bp open reading frame which encoded a protein of 572 amino acids.Amino acid sequence homology alignment and phylogenetic analysis demonstrated that AsHMGR1 belonged to the HMGR gene family.The detection of tissue expression patterns showed that AsHMGR1 was mainly expressed in the stem,followed by roots and branches.AsHMGR1 could be stimulated by methyl jasmonate and H2O2to varying degrees in a time-dependent manner.Conclusion These data will provide a foundation for further investigation on AsHMGR1 functions and regulatory mechanisms in sesquiterpene synthesis in A.sinensis. | XU Yan-hong YANG Xin WEI Jian-he ZHANG Xing-li ZHANG Zheng GAO Zhi-hui SUI Chun | 2013 | Chinese Herbal Medicines2013，5，3： | 6 |
| 18 | Synthesis of graphene materials by electrochemical exfoliation: Recent progress and future potential显示文摘Synthesis of structurally controlled graphene materials is critical for realizing their practical applications.The electrochemical exfoliation of graphite has emerged as a simple method to produce graphene materials.This review examines research progress in the last 5 years,from 2015 to 2019.Graphene material synthesis methods generally have a trade‐off between increasing production yield and achieving better material property control.The synthesis conditions for synthesizing pristine graphene,graphene oxide(GO),and graphene composites are significantly different.Thus,in this review,we first discuss synthesis methods for graphene materials with high C/O ratios from four aspects:graphite electrodes,equipment engineering,electrolytes,and additional reduction methods.Next,we survey synthesis methods for GO and examine how the pretreatment of the graphite electrodes,electrolytes,and operation parameters,such as applied voltages,electrolyte temperatures,and mechanical forces,affect the quality of GO.Further,we summarize electrochemical exfoliation methods used to dope graphene materials,introduce covalent functional groups,incorporate various nanoparticles,and assembly of graphene architectures.For all synthesis methods,we compare the properties of resulting graphene materials such as C/O ratios,lateral size,layer numbers,and quality characterized by Raman spectroscopy.Lastly,we propose our perspectives on further research.We hope this review stimulates more studies to realize the on‐demand production of graphene materials with desired properties using electrochemical exfoliation methods. | Fei Liu Chaojun Wang Xiao Sui Muhammad Adil Riaz Meiying Xu Li Wei Yuan Chen | 2019 | Carbon Energy2019，1，2： | 6 |
| 19 | Study on the resolution of multi-aircraft flight conflicts based on an IDQN显示文摘With the rapid growth of flight flow,the workload of controllers is increasing daily,and handling flight conflicts is the main workload.Therefore,it is necessary to provide more efficient conflict resolution decision-making support for controllers.Due to the limitations of existing methods,they have not been widely used.In this paper,a Deep Reinforcement Learning(DRL)algorithm is proposed to resolve multi-aircraft flight conflict with high solving efficiency.First,the characteristics of multi-aircraft flight conflict problem are analyzed and the problem is modeled based on Markov decision process.Thus,the Independent Deep Q Network(IDQN)algorithm is used to solve the model.Simultaneously,a’downward-compatible’framework that supports dynamic expansion of the number of conflicting aircraft is designed.The model ultimately shows convergence through adequate training.Finally,the test conflict scenarios and indicators were used to verify the validity.In 700 scenarios,85.71%of conflicts were successfully resolved,and 71.51%of aircraft can reach destinations within 150 s around original arrival times.By contrast,conflict resolution algorithm based on DRL has great advantages in solution speed.The method proposed offers the possibility of decision-making support for controllers and reduce workload of controllers in future high-density airspace environment. | Dong SUI Weiping XU Kai ZHANG | 2022 | Chinese Journal of Aeronautics2022，35，2： | 6 |
| 20 | Rapid Assessment on Potential Risks of Schistosomiasis Transmission—7 PLADs,China,2019 and 2021显示文摘Summary What is already known about this topic?Oncomelania hupensis(O.hupensis)and livestock are main infection sources of schistosomiasis.The schistosome infected O.hupensis and livestock’s feces are important risk factors in the transmission of schistosomiasis.What is added by this report?The potential risks of schistosomiasis transmission remain prevalent,giving an early warning to local government with information on existing transmission risks.It is expected that the effectiveness and efficiency of schistosomiasis surveillance could be improved by conducting rapid risk assessment at the beginning of transmission season. | Junyi He Hongqing Zhu Ziping Bao Lijuan Zhang Yinlong Li Ting Feng Suying Guo Wangping Deng Can Wang Hui Dang Tiewu Jia Chao Lyu Zhiqiang Qin Chunli Cao Jing Xu Shizhu Li Xiaonong Zhou | 2021 | China CDC weekly2021，3，51： | 5 |
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