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| 1 | Prognostic value and nomograms of proximal margin distance in gastric cancer with radical distal gastrectomy显示文摘Objective:The proximal margin(PM)distance for distal gastrectomy(DG)of gastric cancer(GC)remains controversial.This study investigated the prognostic value of PM distance for survival outcomes,and aimed to combine clinicopathologic variables associated with survival outcomes after DG with different PM distance for GC into a prediction nomogram.Methods:Patients who underwent radical DG from June 2004 to June 2014 at Department of General Surgery,Nanfang Hospital,Southern Medical University were included.The first endpoints of the prognostic value of PM distance(assessed in 0.5 cm increments)for disease-free survival(DFS)and overall survival(OS)were assessed.Multivariate analysis by Cox proportional hazards regression was performed using the training set,and the nomogram was constructed,patients were chronologically assigned to the training set for dates from June 1,2004 to January 30,2012(n=493)and to the validation set from February 1,2012 to June 30,2014(n=211).Results:Among 704 patients with p TNM stage I,p TNM stage II,T1-2,T3-4,N0,differentiated type,tumor size≤5.0 cm,a PM of(2.1-5.0)cm vs.PM≤2.0 cm showed a statistically significant difference in DFS and OS,while a PM>5.0 cm was not associated with any further improvement in DFS and OS vs.a PM of 2.1-5.0 cm.In patients with p TNM stage III,N1,N2-3,undifferentiated type,tumor size>5.0 cm,the PM distance was not significantly correlated with DFS and OS between patients with a PM of(2.1-5.0)cm and a PM≤2 cm,or between patients with a PM>5.0 cm and a PM of(2.1-5.0)cm,so there were no significant differences across the three PM groups.In the training set,the C-indexes of DFS and OS,were 0.721 and 0.735,respectively,and in the validation set,the C-indexes of DFS and OS,were 0.752 and 0.751,respectively.Conclusions:It is necessary to obtain not less than 2.0 cm of PM distance in early-stage disease,while PM distance was not associated with long-term survival in later and more aggressive stages of disease because more advanced GC is a systemic disease.Different types of patients should be considered for removal of an individualized PM distance intra-operatively.We developed a universally applicable prediction model for accurately determining the 1-year,3-year and 5-year DFS and OS of GC patients according to their preoperative clinicopathologic characteristics and PM distance. | Jun Luo Yuming Jiang Xinhua Chen Yuehong Chen Jhang Lopsang Gurung Tingyu Mou Liying Zhao Guoqing Lyu Tuanjie Li Guoxin Li Jiang Yu | 2020 | Chinese Journal of Cancer Research2020，32，2： | 8 |
| 2 | Verification and fine-mapping of QTLs conferring days to flowering in soybean using residual heterozygous lines显示文摘The results of QTL mapping based on a primary mapping population should be further verified and refined for its real utilization in marker-assisted selection or map-based cloning.The primary mapping population contains 114 BC1F1 plants of the backcross between Essex (maturity group V,MG V) as the donor parent and ZDD2315 (MG II) as the recurrent parent.In this study,a genetic linkage map with 250 SSR markers spanning a total length of 2963.5 cM on 25 linkage groups (LG) was constructed using software MAPMAKER3.0.Six kinds of genetic statistical models of 4 softwares,i.e.WinQTL Cartographer Version 2.5,IciMapping Version 2.0,MapQTL Version 5.0 and QTLnetwork Version 2.0,were used to map QTLs conferring days to flowering of the BC1F3 lines.Nine QTLs were mapped on 6 different linkage groups (LG).Of those,6 QTLs were detected by at least two different genetic statistical models,while the other three were detected by only one procedure.Among the three QTLs,Flwdt7 was mapped between Sat_213 and Satt643 on LG C2 with only 11.0% contribution rate.For confirmation of Flwdt7,5 RHL populations were developed through selfing eight BC1F5 plants heterozygous at seven markers around the locus.The RHL populations with the same segregating loci were bulked and used to construct a secondary linkage map of the specific segment using software JoinMap 3.0.The genetic distances among the markers on the specific segment became shorter than those of the whole genome map.On the secondary map,Flwdt7 was mapped between Satt277 and Satt489,next to its primary interval Sat_213-Satt643,with distance 1.40 cM to Satt277 and 0.45 cM to Satt489,confidence interval narrowed to 2.7 cM,and contribution rate increased to 36.8%.The results were confirmed with significance analysis among marker genotypes on individual loci and comparison analysis of target marker intervals among near isogenic lines (plants).Thus the strategy by using residual heterozygous lines for QTL fine-mapping on target segments based on primary whole genome scanning with multiple mapping models was demonstrated to be effective. | SU ChengFu LU WeiGuo ZHAO TuanJie GAI JunYi | 2010 | Chinese Science Bulletin2010，55，6： | 6 |
| 3 | Genetic diversity,geographic differentiation and evolutionary relationship among ecotypes of Glycine max and G. soja in China显示文摘The knowledge of origin and evolution of cultivated soybeans is one of the basic issues in both biology and agronomy of the crop. In order to investigate the nuclear and cytoplasmic genetic diversity, geographic differentiation and genetic relationship among geographic ecotypes of cultivated (Glycine max) and wild (G. soja) soybeans, the allelic profiles at 60 nuclear simple-sequence repeat (nuSSR) loci and 11 chloroplastic SSR (cpSSR) loci evenly distributed on whole genome of 393 landraces and 196 wild accessions from nation-wide growing areas in China were analyzed. (i) The genetic diversity of the wild soybean was obviously larger than that of the cultivated soybean, with their nuSSR and cpSSR alleles as 1067 vs. 980 and 57 vs 44, respectively. Of the 980 nuclear alleles detected in the cultivated soybean, 377 new ones (38.5%) emerged, while of the 44 chloroplastic alleles in the cultivated soybean, seven new ones (15.9%) emerged after domestication. (ii) Among the cultivated geographic ecotypes, those from southern China, including South-Central China, Southwest China and South China possessed relatively great genetic diversity than those from northern China, while among the wild geographic ecotypes, the Middle and Lower Changjiang Valleys wild ecotype showed the highest genetic diversity. (iii) The analysis of molecular variance, association analysis between geographic grouping and molecular marker clustering and analysis of specific-present alleles of ecotypes demonstrated that the geographic differentiation of both cultivated and wild soybeans associated with their genetic differentiation, or in other words, had their relevant genetic bases. (iv) The cluster analysis of all accessions clearly showed that the wild accessions from Middle and Lower Changjiang Valleys and South-Central & Southwest China had relatively small genetic distances with all cultivated accessions. The UPGMA dendrogram among geographic ecotypes further showed that the genetic distances between all cultivated ecotypes and the Middle and Lower Changjiang Valleys wild ecotype were smaller than those with other wild ones, including their local wild counterparts. Therefore, it is inferred that the wild ancestors in southern China, especially those from Middle and Lower Changjiang Valleys might be the common ancestor of all the cultivated soybeans. | WEN ZiXiang, ZHAO TuanJie, DING YanLai & GAI JunYi Soybean Research Institute of Nanjing Agricultural University National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China | 2009 | Chinese Science Bulletin2009，54，23： | 5 |
| 4 | A β-glucosidase-producing M-2 strain: Isolation from cow dung and fermentation parameter optimization for flaxseed cake显示文摘Flaxseed cake contains cyanogenic glucosides, which can be metabolized into hydrocyanic acid in an animal's body, leading to asphyxia poisoning in cells. Beta-glucosidase is highly efficient in degrading cyanogenic glucosides. The Cattle may have b-glucosidase-producing strains in the intestinal tract after eating small amounts of flaxseed cake for a long time. This study aimed to isolate of a strain from cow dung that produces b-glucosidase with high activity and can significantly reduce the amount of cyanogenic glucosides. We used cow dung as the microflora source and an esculin agar as the selective medium. After screening with 0.05% esculin and 0.01% ferric citrate, we isolated 5 strains producing high amounts of b-glucosidase. In vitro flaxseed cake fermentation was fermented by these 5 strains, in which the strain M-2 exerted the best effect(P < 0.05). The strain M-2 was identified as Lichtheimia ramosa and used as the fermentation strain to optimize the fermentation parameters by a single factor analysis and orthogonal experimental design. The optimum condition was as follows: inoculum size3%, water content 60%, time 144 h, and temperature 32℃. Under this condition, the removal rate of cyanogenic glucosides reached 89%, and crude protein increment reached 44%. These results provided a theoretical basis for the removal of cyanogenic glucosides in flaxseed and the comprehensive utilization of flaxseed cake. | Chenhui Li Junshu Wei Yaping Jing Baoxia Teng Pingrong Yang Xinjun Chen Haiying Huang Tang Zhao Tuanjie Che Chunjiang Zhang | 2019 | Animal Nutrition2019，，1： | 5 |
| 5 | Exploration of presence/absence variation and corresponding polymorphic markers in soybean genome显示文摘This study was designed to reveal the genome‐wide distribution of presence/absence variation(PAV) and to establish a database of polymorphic PAV markers in soybean. The 33 soybean whole‐genome sequences were compared to each other with that of Williams 82 as a reference genome. A total of 33,127 PAVs were detected and 28,912 PAV markers with their primer sequences were designed as the database NJAUSoyPAV_1.0. The PAVs scattered on whole genome while only 518(1.8%) overlapped with simple sequence repeats(SSRs) in BARCSOYSSR_1.0database. In a random sample of 800 PAVs, 713(89.13%) showed polymorphism among the 12 differential genotypes. Using 126 PAVs and 108 SSRs to test a Chinese soybean germplasm collection composed of 828 Glycine soja Sieb. et Zucc. and Glycine max(L.) Merr. accessions, the per locus allele number and its variation appeared less in PAVs than in SSRs. The distinctness among alleles/bands of PCR(polymerase chain reaction) products showed better in PAVs than in SSRs, potential in accurate marker‐assisted allele selection. The association mapping results showed SSR t PAV was more powerful than any single marker systems.The NJAUSoyPAV_1.0 database has enriched the source of PCR markers, and may fit the materials with a range of per locus allele numbers, if jointly used with SSR markers. | Yufeng Wang Jiangjie Lu Shouyi Chen Liping Shu Reid G.Palmer Guangnan Xing Yan Li Shouping Yang Deyue Yu Tuanjie Zhao Junyi Gai | 2014 | Journal of Integrative Plant Biology2014，56，10： | 5 |
| 6 | Mapping Quantitative Trait Loci Associated with Aluminum Toxin Tolerance in NJRIKY Recombinant Inbred Line Population of Soybean (Glycine max)显示文摘To investigate the genetic mechanism of Al-tolerance in soybean, a recombinant inbred line population (RIL) with 184 F2:7:11 lines derived from the cross of Kefeng No.1 × Nannong 1138-2 (Al-tolerant × Al-sensitive) were tested in pot experiment with sand culture medium in net room in Nanjing. Four traits, i.e. plant height, number of leaves, shoot dry weight and root dry weight at seedling stage, were evaluated and used to calculate the average membership index (FAi) as the indicator of Al-tolerance. The composite interval mapping (CIM) under WinQTL Cartographer v. 2.5 detected five QTLs (i.e. qFAi-1, qFAi- 2, qFAi-3, qFAi-4 and qFAi-5), explaining 5.20%-9.07% of the total phenotypic variation individually. While with the multiple interval mapping (MIM) of the same software, five QTLs (qFAi-1, qFAi-5, qFAi-6, qFAi-7, and qFAi-8) explaining 5.7%-24.60% of the total phenotypic variation individually were mapped. Here qFAi-1 and qFAi-5 were detected by both CIM and MIM with the locations in a same ?anking marker region, GMKF046-GMKF080 on B1 and satt278-sat_95 on L, respectively. While qFAi-2 under CIM and qFAi-6 under MIM both on D1b2 were located in neighboring regions with their confidence intervals overlapped and might be the same locus. Segregation analysis under major gene plus polygene inheritance model showed that Al-tolerance was controlled by two major genes (h2mg = 33.05%) plus polygenes (h2pg = 52.73%). Both QTL mapping and segregation analysis confirmed two QTLs responsible for Al-tolerance with relatively low heritability, and there might be a third QTL, confounded with the polygenes in segregation analysis. | Bo Qi Paul Korir Tuanjie Zhao Deyue Yu Shouyi Chen Junyi Gai | 2008 | Journal of Integrative Plant Biology2008，50，9： | 4 |
| 7 | Detecting the QTL-allele system controlling seed-flooding tolerance in a nested association mapping population of soybean显示文摘Soil flooding stress,including seed-flooding,is a key issue in soybean production in highrainfall and poorly drained areas.A nested association mapping(NAM)population comprising 230 lines of two recombinant inbred line(RIL)populations with a common parent was established and tested for seed-flooding tolerance using relative seedling length as indicator in two environments.The population was genotyped using RAD-seq(restriction site-associated DNA sequencing)to generate 6137 SNPLDB(SNP linkage disequilibrium block)markers.Using RTM-GWAS(restricted two-stage multi-locus multiallele genome-wide association study),26 main-effect QTL with 63 alleles and 12 QEI(QTL×environment)QTL with 27 alleles in a total of 33 QTL with 78 alleles(12 dual-effect alleles)were identified,explaining respectively 50.95%and 14.79%of phenotypic variation.The QTL-alleles were organized into main-effect and QEI matrices to show the genetic architecture of seed-flooding tolerance of the three parents and the NAM population.From the main-effect matrix,the best genotype was predicted to have genotypic value 1.924,compared to the parental value range 0.652–1.069,and 33 candidate genes involved in six biological processes were identified and confirmed byχ2 test.The results may provide a way to match the breeding by design strategy. | Muhammad Jaffer Ali Guangnan Xing Jianbo He Tuanjie Zhao Junyi Gai | 2020 | The Crop Journal2020，8，5： | 3 |
| 8 | Aircraft Measurements of the Microphysical Properties of Stratiform Clouds with Embedded Convection显示文摘The presence of embedded convection in stratiform clouds strongly affects ice microphysical properties and precipitation formation.In situ aircraft measurements,including upward and downward spirals and horizontal penetrations,were performed within both embedded convective cells and stratiform regions of a mixedphase stratiform cloud system on 22 May 2017.Supercooled liquid water measurements,particle size distributions,and particle habits in different cloud regions were discussed with the intent of characterizing the riming process and determining how particle size distributions vary from convective to stratiform regions.Significant amounts of supercooled liquid water,with maxima up to 0.6 g m−3,were observed between−3℃ and−6℃ in the embedded convective cells while the peak liquid water content was generally less than 0.1 g m−3 in the stratiform regions.There are two distinct differences in particle size distributions between convective and stratiform regions.One difference is the significant shift toward larger particles from upper−15℃ to lower−10℃ in the convective region,with the maximum particle dimensions increasing from less than 6000μm to over 1 cm.The particles larger than 1 cm at−10℃ are composed of dendrites and their aggregates.The other difference is the large concentrations of small particles(25–205μm)at temperatures between−3℃ and−5℃ in the convective region,where rimed ice particles and needles coexist.Needle regions are observed from three of the five spirals,but only the cloud conditions within the convective region fit into the Hallett-Mossop criteria. | Tuanjie HOU Hengchi LEI Youjiang HE Jiefan YANG Zhen ZHAO Zhaoxia HU | 2021 | Advances in Atmospheric Sciences2021，38，6： | 3 |
| 9 | An optimized protocol for stepwise optimization of real-time RT-PCR analysis显示文摘Computational tool-assisted primer design for real-time reverse transcription(RT)PCR(qPCR)analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.It can lead to false confidence in the quality of the designed primers,which sometimes results in skipping the optimization steps for qPCR.However,the optimization of qPCR parameters plays an essential role in the efficiency,specificity,and sensitivity of each gene’s primers.Here,we proposed an optimized approach to sequentially optimizing primer sequences,annealing temperatures,primer concentrations,and cDNA concentration range for each reference(and target)gene.Our approach started with a sequence-specific primer design that should be based on the single-nucleotide polymorphisms(SNPs)present in all the homologous sequences for each of the reference(and target)genes under study.By combining the efficiency calibrated and standard curve methods with the 2−ΔΔCt method,the standard cDNA concentration curve with a logarithmic scale was obtained for each primer pair for each gene.As a result,an R 2≥0.9999 and the efficiency(E)=100±5% should be achieved for the best primer pair of each gene,which serve as the prerequisite for using the 2^(−ΔΔCt) method for data analysis.We applied our newly developed approach to identify the best reference genes in different tissues and at various inflorescence developmental stages of Tripidium ravennae,an ornamental and biomass grass,and validated their utility under varying abiotic stress conditions.We also applied this approach to test the expression stability of six reference genes in soybean under biotic stress treatment with Xanthomonas axonopodis pv.glycines(Xag).Thus,these case studies demonstrated the effectiveness of our optimized protocol for qPCR analysis. | Fangzhou Zhao Nathan A.Maren Pawel Z.Kosentka Ying-Yu Liao Hongyan Lu James R.Duduit Debao Huang Hamid Ashrafi Tuanjie Zhao Alejandra I.Huerta Thomas G.Ranney Wusheng Liu | 2021 | Horticulture Research2021，8，1： | 2 |
| 10 | HIGH-ACCURACY BAND TO BAND REGISTRATION METHOD FOR MULTI-SPECTRAL IMAGES OF HJ-1A/B显示文摘Band-to-band registration accuracy is an important parameter of multispectral data. A novel band-to-band registration approach with high precision is proposed for the multi-spectral images of HJ-1A/B. Firstly, the main causes resulted in misregistration are analyzed, and a high-order polynomial model is proposed. Secondly, a phase fringe filtering technique is employed to Phase Correlation Method based on Singular Value Decomposition (SVD-PCM) for reducing the noise in phase difference matrix. Then, experiments are carried out to build nonlinear registration models, and images of green band and red band are aligned to blue band with an accuracy of 0.1 pixels, while near infrared band with an accuracy of 0.2 pixels. | Lu Hao Liu Tuanjie Zhao Haiqing | 2012 | Journal of Electronics(China)2012，29，5： | 2 |
| 11 | Microphysical characteristics of precipitating cumulus cloud based on airborne Ka-band cloud radar and droplet measurements显示文摘在积云中,大多数云粒子的直径在7到10微米之间,而在层云中,大多数云粒子的直径不超过2微米.云滴有效半径与云中行星边界层(PBL)及PBL上层的气溶胶数浓度(Na)呈负相关.在1500米以上的高液态水含量区域,云滴浓度(Nc)变化不大,Na含量降低.高雷达反射率对应于大的FCDP云粒子浓度和小的气溶胶粒子浓度.积云中的强上升气流增加了云滴的峰值半径和数浓度,拓宽了云滴谱;较低的空气温度有利于粒子的凝结增长,并产生较大的液滴. | Lei Wei Mengyu Huang Rong Zhang Yuhuan Lü Tuanjie Hou Hengchi Lei Delong Zhao Wei Zhou Yuan Fu | 2022 | Atmospheric and Oceanic Science Letters2022，15，2： | 1 |
| 12 | Geographic differentiation and phylogeographic relationships among world soybean populations显示文摘A fast-growing protein and oil crop,soybean was domesticated in ancient China and disseminated early in Asia and afterwards to other continents,in particular the Americas in recent centuries.After adaptation,locally developed landraces and cultivars formed a diversity of geographic-populations.In an investigation of their phylogeographic features,marker-derived traits were combined with geography-related photo-and temperaturesensitive traits to study 13 geographic-populations comprising 371 accessions.Extreme differentiation among geographic-populations was observed for flowering date(33–94 days),maturity date(79–181 days),and main stem node number(6–25 nodes).Restriction-site associated DNA sequencing revealed strong genetic differentiation among these geographic-populations,including genetic richness(alleles,35,242–44,986)and specific-present alleles(SPAs,0–67).More SPAs(28–67)emerged in some secondary and tertiary centers than in centers of origin(8–11).Phenotypic and genotypic clustering divided 11 of the 13 geographic-populations into the same five sets of sensitivity-similar geographic-populations and grouped the populations of northeast China and northern North America rather than center-of-origin populations as secondary centers,indicating the importance of geography-related traits in determining genetic differences among geographic-populations.A model of four soybean dissemination paths is presented:from the center of origin to the north,east,and south in Asia and from northeast China to Europe and the Americas.These findings provide a detailed phylogeographic understanding of worldwide soybeans. | Xueqin Liu Jianbo He Yufeng Wang Guangnan Xing Yan Li Shouping Yang Tuanjie Zhao Junyi Gai | 2020 | The Crop Journal2020，8，2： | 1 |
| 13 | Domestication or Foreignization:A Cultural Choice——On the Nickname Translation in Sidney Shapiro’s Outlaws of the Marsh显示文摘Through detailed statistics and analysis about the tr anslation of 108 her oes’nicknames,the author manages to probe into the rendering of nicknames in Outlaws of the Marsh fr om the angle of foreignization and domestication theory.And in Outlaws of the Marsh,Sidne y Shapiro managed to achieve a balance between domestication and foreignization in translating the nicknames. | Zhao Tuanjie | 2020 | 校园英语2020，，1： | 0 |
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