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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | GPS-constrained inversion of present-day slip rates along major faults of the Sichuan-Yunnan region, China显示文摘A linked-fault-element model is employed to invert for contemporary slip rates along major active faults in the Sichuan-Yunnan region (96°-108°E, 21°-35°N) using the least squares method. The model is based on known fault geometry, and constrained by a GPS-derived horizontal velocity field. Our results support a model attributing the eastward extrusion of the Tibetan Plateau driven mainly by the north-northeastward indentation of the Indian plate into Tibet and the gravitational collapse of the plateau. Resisted by a relatively stable south China block, materials of the Sichuan-Yunnan region rotate clockwise around the eastern Himalayan tectonic syntaxis. During the process the Garzê-Yushu, Xianshuihe, Anninghe, Zemuhe, Daliangshan, and Xiaojiang faults, the southwest extension of the Xiaojiang fault, and the Daluo-Jinghong and Mae Chan faults constitute the northeast and east boundaries of the eastward extrusion, with their left slip rates being 0.3-14.7, 8.9-17.1, 5.1 ± 2.5, 2.8 ± 2.3, 7.1 ± 2.1, 9.4 ± 1.2, 10.1 ± 2.0, 7.3 ± 2.6, and 4.9 ± 3.0 mm/a respectively. The southwestern boundary consists of a widely distributed dextral transpressional zone other than a single fault. Right slip rates of 4.2 ± 1.3, 4.3 ± 1.1, and 8.5 ± 1.7 mm/a are detected across the Nanhua-Chuxiong-Jianshui, Wuliangshan, and Longling-Lancang faults. Crustal deformation across the Longmenshan fault is weak, with short-ening rates of 1.4 ± 1.0 and 1.6 ± 1.3 mm/a across the Baoxing-Beichuan and Beichuan-Qingchuan segments. Northwest of the Longmenshan fault lies an active deformation zone (the Longriba fault) with 5.1±1.2 mm/a right slip across. Relatively large slip rates are detected across a few faults within the Sichuan-Yunnan block: 4.4±1.3 mm/a left slip and 2.7±1.1 mm/a shortening across the Litang fault, and 2.7±2.3 mm/a right-lateral shearing and 6.7±2.3 mm/a shortening across the Yunongxi fault and its surrounding regions. In conclusion, we find that the Sichuan-Yunnan region is divided into more than a dozen active micro-blocks by a large number of faults with relatively slow slip rates. The eastward extrusion of the Tibetan Plateau is absorbed and adjusted in the region mainly by these faults, other than a small number of large strike-slip faults with fast slip rates. | WANG YanZhao WANG EnNing SHEN ZhengKang WANG Min GAN WeiJun QIAO XueJun MENG GuoJie LI TieMing TAO Wei YANG YongLin CHENG Jia LI Peng | 2008 | Science China Earth Sciences2008，51，9： | 65 |
| 3 | TALEN-based generation of a cynomolgus monkey disease model for human microcephaly显示文摘基因在非人类的首领编辑可以为在人探索病原学和遗传上基于的神经病学的混乱的治疗学的策略导致珍贵模型。然而,仔细在人模仿病理学、行为的赤字的神经病学的混乱的一个猴子模型成功地还没被产生了。Microcephalin 1 (MCPH1 ) 在人的大脑,和智力迟钝伴随的 MCPH1 变化原因头小畸型的进化被含有。这里,我们产生了用抄写带 biallelic MCPH1 变化的一只 cynomolgus 猴子(Macaca fascicularis ) 像使活跃之物的受动器核酸酶。猴子概括了大多数在病人观察的重要临床的特征,包括在头圆周,早熟的染色体冷凝作用(PCC ) ,语料库 callosum 的发育不全和上面的手足 spasticity 的显著减小。而且,在变异的真皮的成纤维细胞的 MCPH1 的 overexpression 救了 PCC 症候群。这个猴子模型可以帮助我们阐明在人的头小畸型并且更好的致病的 MCPH1 的角色在首领大脑尺寸的进化理解这蛋白质的功能。 | Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Jie Ren Xiaofeng Lin Haiqing Zheng Weijun Huang Yunhan Ma Dongdong Xu Zheng Chen Xinming Song Xinyi Lin Min Zhuang Tao Wang Fengfeng Zhuang Jianzhong Xi Frank Fuxiang Mao Huimin Xia Bruce T Lahn Qi Zhou Shihua Yang Andy Peng Xiang | 2016 | Cell Research2016，26，9： | 13 |
| 4 | Human mesenchymal stromal cells enhance the immunomodulatory function of CD8+CD28- regulatory T cells显示文摘 | Qiuli Liu Haiqing Zheng Xiaoyong Chen Yanwen Peng Weijun Huang Xiaobo Li Gang Li Wenjie Xia Qiquan Sun an Andy Peng Xiang | 2015 | Cellular & Molecular Immunology2015，12，6： | 12 |
| 5 | Enhancement of Fenton oxidation for removing organic matter from hypersaline solution by accelerating ferric system with hydroxylamine hydrochloride and benzoquinone显示文摘Fenton oxidation is generally inhibited in the presence of a high concentration of chloride ions.This study investigated the feasibility of using benzoquinone(BQ) and hydroxylamine hydrochloride(HA) as Fenton enhancers for the removal of glycerin from saline water under ambient temperature by accelerating the ferric system.It was found that organics removal was not obviously affected by chloride ions of low concentration(less than 0.1 mol/L),while the mineralization rate was strongly inhibited in the presence of a large amount of chloride ions.In addition,ferric hydrolysis-precipitation was significantly alleviated in the presence of HA and BQ,and HA was more effective in reducing ferric ions into ferrous ions than HA,while the H_2O_2 decomposition rate was higher in the BQ-Fenton system.Electron spin resonance analysis revealed that OH production was reduced in high salinity conditions,while it was enhanced after the addition of HA and BQ(especially HA).This study provided a possible solution to control and alleviate the inhibitory effect of chloride ions on the Fenton process for organics removal. | Siwei Peng Weijun Zhang Jie He Xiaofang Yang Dongsheng Wang Guisheng Zeng | 2016 | Journal of Environmental Sciences2016，28，3： | 10 |
| 6 | Fluid–solid coupling analysis of rock pillar stability for concealed karst cave ahead of a roadway based on catastrophic theory显示文摘In order to study the mechanism of water inrush from a concealed, confined karst cave, we established a fluid–solid coupling model of water inrush from a concealed karst cave ahead of a roadway and a strength reduction method in a rock pillar for preventing water inrush based on catastrophic theory. Fluid–solid coupling effects and safety margins in a rock pillar were studied. Analysis shows that rock pillar instability, exerted by disturbance stress and seepage stress, is the process of rock pillar catastrophic destabilization induced by nonlinear extension of plastic zones in the rock pillar. Seepage flow emerges in the rock pillar for preventing water inrush, accompanied by mechanical instability of the rock pillar. Taking the accident of a confined karst cave water-inrush of Qiyi Mine as an example, by studying the safety factor of the rock pillar and the relationship between karst cave water pressure and thickness of the rock pillar,it is proposed that rock pillar thickness with a safety factor equal to 1.5 is regarded as the calculated safety thickness of the rock pillar, which should be equal to the sum of the blasthole depth, blasting disturbance depth and the calculated safety thickness of the rock pillar. The cause of the karst water inrush at Qiyi Mine is that the rock pillar was so small that it did not possess a safety margin. Combining fluid–solid coupling theory, catastrophic theory and strength reduction method to study the nonlinear mechanical response of complicated rock engineering, new avenues for quantitative analysis of rock engineering stability evaluation should be forthcoming. | Zhao Yanlin Peng Qingyang Wan Wen Wang Weijun Chen Bin | 2014 | International Journal of Mining Science and Technology2014，24，6： | 9 |
| 7 | Changes in biodiversity and ecosystem function during the restoration of a tropical forest in south china显示文摘Tropical forests continue to vanish rapidly,but few long-term studies have ever examined if and how the lost forests can be restored.Based on a 45-year restoration study in south China,we found that a tropical rain forest,once completely destroyed,could not recover naturally without deliberate restoration efforts.We identified two kinds of thresholds that must be overcome with human ameliorative measures before the ecosystem was able to recover.The first threshold was imposed primarily by extreme physical conditions such as exceedingly high surface temperature and impoverished soil,while the second was characterized by a critical level of biodiversity and a landscape context that accommodates dispersal and colonization processes.Our three treatment catchments(un-restored barren land,single-species plantation,and mixed-forest stand)exhibited dramatically different changes in biodiversity and ecosystem functioning over 4 decades.The mixed forest,having the highest level of biodiversity and ecosystem functioning,possesses several major properties of tropical rain forest.These findings may have important implications for the restoration of many severely degraded or lost tropical forest ecosystems. | REN Hai,LI ZhiAn,SHEN WeiJun,YU ZuoYue,PENG ShaoLin,LIAO ChongHui,DING MingMao & WU JianGuo 1 South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China 2 Institute of Ecology and Evolution,Zhongshan University,Guangzhou 510275,China 3 Guangdong Institute of Entomology,Guangzhou 510275,China 4 School of Life Sciences and Global Institute of Sustainability,Arizona State University,Tempe,AZ 85287,USA | 2007 | Science China(Life Sciences)2007，50，2： | 9 |
| 8 | Characteristics of deformation and stress distribution of small coa pillars under leading abutment pressure显示文摘Based on the engineering project on a small coal pillar of 12,521 working face roadway in Xieqiao Coalmine, data regarding surface displacements of the coal pillar, deep displacements and mining stress have been collected and analyzed. The results show that macroscopic transverse fractures of the inner coal pillar are developed within 2–4 m of the roadway surface, which is located outside the coal pillar anchorage zone. There is a displacement of 530 mm at the monitoring point in the 6 m deep zone of the pillar. Transfer of the fracture zone is found in a small coal pillar and the fractures within 3–4 m of the coal-rock zone from the roadway surface undergo propagation and closure of cracks which means this fracture zone is transferred from 3–4 m outside the roadway to only 2–3 m from the roadway surface. In the monitoring zone, vertical and horizontal stresses increase with a feature that shows that acceleration in the deep zone of the pillar is greater than that in the shallow zone. Furthermore, the acceleration of vertical stress is also greater than that of horizontal stress with a peak value in the 4 m zone.The research findings provide a reference for the regulation of a reasonable width of coal pillar in coalmines and optimal control design of surrounding rock. | Wu Hai Zhang Nong Wang Weijun Zhao Yiming Cao Peng | 2015 | International Journal of Mining Science and Technology2015，25，6： | 6 |
| 9 | Microwave strengthens decomposition of mixed rare earth concentrate: Microwave absorption characteristics显示文摘The potential application of microwave heating technology for processing the mixed rare earth concentrate was systematically investigated by analyzing the microwave absorption characteristics in this study. The complex permittivity was measured through resonant cavity perturbation method. The variations of permittivity, the loss factor, loss tangent and the penetration depth with the increasing temperature were investigated numerically. The results indicate that the permittivity increases as the temperature increases, and temperature has a pivotal effect on it. The mixed concentrate is high loss material at the temperature range from 600 to 800℃ according to theoretical analyses of loss tangent and penetration depth. The results of phase transition analysis prove that the variation of microwave absorption characteristics of mixed concentrate is caused by the changes of crystal and lattice structures.The reflectivity, loss factor and penetration depth of the mixed concentrate were also calculated, and the results indicate that processing the mixed concentrate by microwave heating is of high feasibility and industrial potential. | Yukun Huang Ting'an Zhang Zhihe Dou Guozhi Lv Guihong Han Weijun Peng | 2019 | Journal of Rare Earths2019，37，5： | 5 |
| 10 | Effects of alkalinity on interaction between EPS and hydroxy-aluminum with different speciation in wastewater sludge conditioning with aluminum based inorganic polymer flocculant显示文摘Extracellular polymeric substances(EPS)form a stable gel-like structure to combine with water molecules through steric hindrance,making the mechanical dewatering of wastewater sludge considerably difficult.Coagulation/flocculation has been widely applied in improving the sludge dewatering performance,while sludge properties(organic fraction and solution chemistry conditions)are highly changeable and have important effects on sludge flocculation process.In this work,the alkalinity effects on sludge conditioning with hydroxy-aluminum were comprehensively investigated,and the interaction mechanisms between EPS and hydroxy-aluminum with different speciation were unraveled.The results showed that the effectiveness of hydroxy-aluminum conditioning gradually deteriorated with increase in alkalinity.Meanwhile,the polymeric hydroxy-aluminum(Al 13)and highly polymerized hydroxy-aluminum(Al 30)were hydrolysed and converted into amorphous aluminum hydroxide(Al(OH)3),which changed the flocculation mechanism from charge neutralization and complexing adsorption to hydrogen bond interaction.Additionally,both Al 13 and Al 30 showed higher binding capacity for proteins and polysaccharides in EPS than monomeric aluminum and Al(OH)3.Al 13 and Al 30 coagulation changed the secondary structure of proteins in EPS,which caused a gelation reaction to increase molecular hydrophobicity of proteins and consequently sludge dewaterability.This study provided a guidance for optimizing the hydroxy-aluminum flocculation conditioning of sludge with high solution alkalinity. | Weijun Zhang Mingyue Tang Dandan Li Peng Yang Shiwei Xu Dongsheng Wang | 2021 | Journal of Environmental Sciences2021，33，2： | 5 |
| 11 | Hardware-in-the-loop simulation for the contact dynamic process of flying objects in space显示文摘在地面上的一个严肃环境,自从人首先由飞目标探索了空间,在空间的零严肃的环境的二个飞的目标的接触过程的模拟是一个挑战性的问题。Hardware-in-the-loop (HIL ) 模拟是一个重要、有效的方法在空间测试真实停靠机制的可用性,可靠性,和安全。为 HIL 模拟系统有四个主要问题:能够高频率反应,高运动精确,高速度,和快速的加速的模拟器的设计;为模拟失真的赔偿;为 HIL 模拟进程的一个控制模型的设计;并且试验性的确认。这里,我们与 6-DOF 3-3 建议一个新奇 HIL 模拟器系统垂直平行机制和 3-DOF 3-PRS 平行机制;发现模拟失真的原则;为模拟器的力量测量系统,动态反应,和结构的动力学介绍失真赔偿模型;并且为 HIL 模拟过程提供一个控制模型。二种实验在被动未受潮的有弹性的杆和停靠机制上被执行测试他们的表演并且验证 HIL 模拟器的有效性和可用性。在这份报纸建议的 HIL 模拟系统为开发停靠的空格是有用的, berthing, refueling,修理,升级,搬运,并且 rescuing 技术。 | GAO Feng QI ChenKun REN AnYe ZHAOXianChao CAO Rui SUN Qiao WANG Qian HU Yan HE Jun JIN ZhenLin LIU RenQiang ZHANG Yong GUO WeiZhong HU ZhenYu TANG Ping NI Bo JING QianFeng WANG WeiJun GAO Peng | 2016 | Science China(Technological Sciences)2016，59，8： | 4 |
| 12 | A zinc finger protein, interacted with cyclophilin,affects root development via IAA pathway in rice显示文摘The plant hormone auxin plays a crucial role in lateral root development. To better understand the molecular mechanisms underlying lateral root formation,an auxin-responsive gene OsCYP2(Os02g0121300) was characterized from rice. Compared to the wild type,OsCYP2-RNAi(RNA interference) lines exhibited distinctive defects in lateral root development. Yeast two-hybrid and glutathione S-transferase pull-down results confirmed that OsCYP2 interacted with a C2HC-type zinc finger protein(OsZFP, Os01g0252900) which is located in the rice nucleus. T_2 OsZFP-RNAi lines had significantly fewer lateral roots than did wild-type plants, which suggests a role for OsCYP2 and OsZFP in regulating lateral root development.Quantitative real-time polymerase chain reaction showed that the expression of certain Aux/IAA(auxin/indole-3-acetic acid) genes was altered in OsCYP2-and Os ZFP-RNAi lines in response to IAA. These findings imply that OsCYP2 and OsZFP participate in IAA signal pathways controlling lateral root development. More importantly, OsIAA11 showed functional redundancy not only in OsCYP2-RNAi lines but also in Os ZFP-RNAi lines, which provides important clues for the elucidation of mechanisms controlling lateral root development in response to auxin. | Peng Cui Hongbo Liu Songlin Ruan Basharat Ali Rafaqat Ali Gill Huasheng Ma Zhifu Zheng Weijun Zhou | 2017 | Journal of Integrative Plant Biology2017，59，7： | 4 |
| 13 | Effects of microtopography on spatial point pattern of forest stands on the semi-arid Loess Plateau,China显示文摘Microtopography may affect the distribution of forests through its effect on rain redistribution and soil water distribution on the semi-arid Loess Plateau,China.In this study,we investigated the characteristics of microtopography on two shady slopes(slope A,5 hm2,uniform slope;slope B,5 hm2,microtopography slope) and surveyed the height,the diameter at breast height and the location(x,y coordinates) of all selected individual trees(Robinia pseudoacacia Linn.,Pyrus betulifolia Bunge,Populus hopeiensis Hu & Chow,Armeniaca sibirica Lam.,Populus simonii Carr.and Ulmus pumila Linn.) on slope A and slope B in the watersheds of Wuqi county,Shaanxi province.Subsequently,the effects of microtopography on the spatial pattern of forest stands were analyzed using Ripley's K(r) function.The results showed that:(1) The maximal aggregation radiuses of the tree species on the uniform slope(slope A) were larger than 40 m,whereas those of the tree species on the microtopography slope(slope B) were smaller than 30 m.(2) On slope B,the spatial association of R.pseudoacacia with P.betulifolia,A.sibirica,P.simonii and U.pumila varied from being strongly negative to positive at microtopography scales.The spatial association of Populus hopeiensis Hu & Chow with U.pumila also varied from being strongly negative to positive at microtopography scales.However,there was no spatial association between P.betulifolia and P.hopeiensis,P.betulifolia and A.sibirica,P.betulifolia and P.simonii,P.betulifolia and U.pumila,P.hopeiensis and A.sibirica,P.hopeiensis and P.simonii,A.sibirica and P.simonii,A.sibirica and U.pumila,and P.simonii and U.pumila.On slope A,the spatial association between tree species were strongly negative.The results suggest that microtopography may shape tree distribution patterns on the semi-arid Loess Plateau. | WeiJun ZHAO Yan ZHANG QingKe ZHU Wei QIN ShuZhen PENG Ping LI YanMin ZHAO Huan MA Yu WANG | 2015 | Journal of Arid Land2015，7，3： | 3 |
| 14 | Experimental Gaussian Boson sampling显示文摘Gaussian Boson sampling(GBS) provides a highly efficient approach to make use of squeezed states from parametric down-conversion to solve a classically hard-to-solve sampling problem. The GBS protocol not only significantly enhances the photon generation probability, compared to standard Boson sampling with single photon Fock states, but also links to potential applications such as dense subgraph problems and molecular vibronic spectra. Here, we report the first experimental demonstration of GBS using squeezed-state sources with simultaneously high photon indistinguishability and collection efficiency.We implement and validate 3-, 4- and 5-photon GBS with high sampling rates of 832, 163 and 23 kHz,respectively, which is more than 4.4, 12.0, and 29.5 times faster than the previous experiments.Further, we observe a quantum speed-up on a NP-hard optimization problem when comparing with simulated thermal sampler and uniform sampler. | Han-Sen Zhong Li-Chao Peng Yuan Li Yi Hu Wei Li Jian Qin Dian Wu Weijun Zhang Hao Li Lu Zhang Zhen Wang Lixing You Xiao Jiang Li Li Nai-Le Liu Jonathan P.Dowling Chao-Yang Lu Jian-Wei Pan | 2019 | Science Bulletin2019，64，8： | 3 |
| 15 | Far-field coseismic displacements associated with the great Sumatra earthquakes of December 26,2004 and March 29,2005 constrained by Global Positioning System显示文摘Based on continuous GPS observations within China as well as global GPS tracking network, a calculation has been made of far-field coseismic displacements associated with the December, 2004 (Mw = 9.3) and March, 2005 (Mw = 8.7) earthquakes. The far-field coseismic displacements are associated with the 2004 shock range more than 6000―7000 km in both north-south and east-west dimensions, and depict an undulated wave pattern of contraction and extension. The coseismic displacements associ-ated with the 2005 event, however, are distributed near the epicentral region, and the event itself may be an aftershock of the 2004 earthquake. | WANG Min ZHANG Peizhen SHEN Zhengkang LIU Jie SUN Hanrong GAN Weijun LI Peng | 2006 | Chinese Science Bulletin2006，51，14： | 3 |
| 16 | Nanomaterials toward the treatment of Alzheimer's disease:Recent advances and future trends显示文摘Alzheimer’s disease(AD)is a progressive and fatal neurodegenerative condition and the most prevalent cause of dementia.This disease is characterized by progressive cognitive impairment.The prevalence of AD is currently affecting more than 35 million people and is rising worldwide.No efficient therapy is currently available due to low drug potency and a number of various obstacles to delivery.Recent nanotechnological advancements have the potential to offer promising therapeutic options.Progress on nanomaterials as well as their applications in biomedicine is receiving increasing attention,especially the advantages of nanomaterial-based drug delivery systems.The aim of this review is to comprehensively summarize the latest developments in nanomaterial-based strategies for AD treatment,including nanoparticles,liposomes and other options for the delivery of therapeutic compounds and scaffolds for cell delivery strategies.Future research directions are also proposed.We hope this review can provide important information to guide the future development of nanomaterials in AD treatment. | Huihui Zeng Yujie Qi Zheyu Zhang Chuntai Liu Weijun Peng Yi Zhang | 2021 | Chinese Chemical Letters2021，32，6： | 2 |
| 17 | Intercellular adhesion molecule-5 protects PAJU cells during in vitro ischemia following cerebral infarction显示文摘BACKGROUND: Intercellular adhesion molecule-5 (ICAM-5) relieves the damage of beta-amyloid protein to PAJU cells. However, little is known about how ICAM-5 works as a neurotrophic factor, or whether ICAM-5 lessens neuronal damage under ischemic conditions following cerebral infarction. OBJECTIVE: To investigate the effects of ICAM-5 on PAJU cells growth in serum-free medium under ischemic conditions following cerebral infarction. DESIGN, TIME AND SETTING: The cytological in vitro study was performed at the Central Laboratory, Second Xiangya Hospital, Central South University, China, in June 2009. MATERIALS: Human ICAM-5 gene transfected into PAJU-TLN cells was supplied by the Life Science College, Helsinki University, Finland. Empty vector transfected PAJU-NEO cells were established by the Gene Center, Second Xiangya Hospital, Central South University, China. METHODS: PAJU-TLN cells transfected with human ICAM-5 or empty vector were incubated in serum-free medium. MAIN OUTCOME MEASURES: Phase contrast microscopy was used to observe changes in PAJU cell morphology. 3-(4, 5-dimethylthiazolzyl)-2, 5-diphenyltetrazolium bromide was used to determine cell viability. Hoechst 33258 was used to stain cell nuclei. Flow cytometry was utilized to measure the apoptosis rate of both PAJU-TLN and PAJU-NEO cells. RESULTS: Both PAJU-TLN and PAJU-NEO cells were injured by cultivating in serum-free medium, but the survival rate of PAJU-TLN cells was significantly higher. CONCLUSION: ICAM-5 protects PAJU-TLN cells from serum deprivation-induced apoptosis, induces the outgrowth of PAJU cells, and diminishes their morphologic impairment. | Weijun Zhou Heping Yang Xiang Wang Yi Peng Dapeng WU Xiaojie Zhang Zhiping Hu | 2010 | Neural Regeneration Research2010，5，3： | 2 |
| 18 | Retrorsine Cooperates with Gut Microbiota to Promote Hepatic Sinusoidal Obstruction Syndrome by Disrupting the Gut Barrier显示文摘Background and Aims:Hepatic sinusoidal obstruction syndrome(HSOS)is a life-threatening syndrome,and a cause is exposure to pyrrolizidine alkaloid(PA)-containing products.It is well-established that retrorsine(RTS),a rep-resentative Pas,insults hepatic sinusoidal endothelial cells and ensues congestion of hepatic sinusoids.However,little known about the impact of Pas on gut microbiota and intesti-nal barrier and inflammation in HSOS.Methods:Mice were gavaged with or without nonabsorbable antibiotics(ABX),followed by a single dose of RTS.The gut microbiota was examined by 16S rDNA sequencing.Results:ABX pretreat-ment significantly reversed RTS-induced liver damage.RTS altered gut microbiota composition,increasing Gram-nega-tive bacteria and resulting in a sharp elevation of circulating lipopolysaccharides(LPS)in HSOS mice.Gut decontamina-tion with ABX alleviated RTS-induced intestine inflamma-tion,protected against disruption of the intestinal epithelial barrier and gut vascular barrier(GVB),and suppressed he-patic LPS-NF-κB pathway activation in RTS-induced HSOS.Importantly,the LPS level was positively correlated with MELD score in patients with HSOS.Elevated LPS in patients with HSOS confirmed that Gram-negative bacteria were in-volved in the pathogenesis of HSOS.Conclusions:RTS,a PA,cooperated with gut dysbiosis to cause intestinal inflam-mation and gut barrier compromise that increased transport of gut-derived LPS into the liver through the portal vein,which contributed to the pathology of HSOS.Modulating the gut microbiota,protecting the intestinal barrier,and sup-pressing intestinal inflammation with prebiotics or antibiot-ics might be a useful pharmacologic intervention in HSOS. | Li Xiao Lilin Hu Huikuan Chu Liuying Chen Jingjing Yan Weijun Wang Xiaoqian Yang Qingjing Zhu Fan Du Yuhu Song Peng Chen Xiaohua Hou Ling Yang | 2022 | Journal of Clinical and Translational Hepatology2022，10，6： | 2 |
| 19 | Laboratory and pilot-scale study on dehydration of benzene by pervaporation显示文摘 | Jiding Li Cuixian Chen Binbing Han Yong Peng Jian Zou Weijun Jiang | 2002 | Journal of Membrane Science2002，，1： | 1 |
| 20 | 溃疡性结肠炎内镜严重度指数(UCEIS)预测溃疡性结肠炎患者行储袋成形术的截点值:多中心长期随访研究结果显示文摘背景:全结直肠切除回肠储袋肛管吻合(IPAA)是溃疡性结肠炎(UC)患者的首选外科治疗术式。溃疡性结肠炎内镜严重度指数(UCEIS)用以预测UC患者的IPAA手术需求,目前尚缺乏数据。本研究旨在建立明确UCEIS预测UC患者IPAA手术需求的截点值。方法:对1986年6月至2020年3月间两个中心收治的UC患者进行回顾性分析。基于入院后首次结肠镜检查结果进行UCEIS评分。采用受试者操作特征曲线分析确定UCEIS评分预测IPAA需求的截点值。结果:293例UC患者纳入研究,中位UCEIS为4分。13年随访期间,80例(28.3%)患者接受外科治疗,其中75例(93.8%)行IPAA手术,5例(6.2%)行次全结肠切除加永久性造口。采用6分作为截点值,UCEIS对IPAA手术需求的预测价值最高,曲线下面积为0.769(P<0.001),灵敏度和特异度分别为72.0%和81.8%。UCEIS≥6是需要行IPAA手术(P<0.001)和恶性转化(P=0.010)的独立预测因素。UCEIS≥6的患者无IPAA生存时间显著短缩(P<0.001)。结论:UCEIS≥6或可作为IPAA手术决策的依据,推荐对这些UC患者行手术治疗以减少恶性转化的发生率。 | Weimin Xu Weijun Ou Jihong Fu Yubei Gu Long Cui Jie Zhong Peng Du | 2021 | Gastroenterology Report2021，9，5： | 1 |
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