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| 1 | Apelin-13 inhibits lipoprotein lipase expression via the APJ/PKCα/miR-361-5p signaling pathway in THP-1 macrophage-derived foam cells显示文摘动脉粥样硬化患者损害被丰富的类脂化合物和长期的发炎的累积描绘。以前的研究显示了导出巨噬细胞的脂蛋白脂肪分解酵素(LPL ) 由加速类脂化合物累积和支持 inflammatory cytokine 分泌物支持动脉粥样硬化前进。尽管 apelin-13 被认为是一个 atheroprotective 因素,它是否能调整 LPL 的表示,仍然保持不清楚。这研究的目的是在 THP-1 导出巨噬细胞的泡沫房间在 LPL 和内在的机制的表示上探索 apelin-13 的效果。Apelin-13 显著地减少了在 10 和 100 nM 的集中的全部的胆固醇,免费胆固醇,和胆固醇酉旨的细胞的层次。ELISA 分析证实有 apelin-13 的处理减少了支持 inflammatory cytokine 分泌物,例如 interleukin-6 (IL-6 ) , interleukin-1 (IL-1 ) 和肿瘤坏死 factor-alpha (TNF-) 。apelin-13 禁止了由西方的污点和即时 PCR 分析揭示了的 LPL 的表示,这也被发现。生物信息学分析和双酶的记者试金直接显示了那 miR-361-5p downregulated 由指向 LPL 的 3UTR 的 LPL 的表示。另外, apelin-13 + miR-361-5p 显著地模仿 downregulated 在房间的 LPL 的表示。最后,我们表明了那 apelin-13 downregulated 通过激活 PKC 的活动的 LPL 的表示。一起拿,我们的结果显示出那 apelin-13 downregulated 经由激活在 THP-1 导出巨噬细胞的泡沫房间表明小径的 APJ/PKC/miR-361-5p 的 LPL 的表示,导致类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制。因此,我们的研究由 apelin-13 提供重要新卓见进类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制,并且在动脉粥样硬化作为一个有希望的治疗学的目标加亮 apelin-13。 | Xin Zhang Qiong Ye Duo Gong Yuan Lv Haipeng Cheng Chong Huang Lingyan Chen Zhenwang Zhao Liang Li Xie Wei Min Zhang Xiaodan Xia Xiaohua Yu Xilong Zheng Shuzhi Wang Zongbao Wang Chaoke Tang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，6： | 6 |
| 2 | Development and characterization of a clinical strain of Coxsackievirus A16 and an eGFP infectious clone显示文摘Coxsackievirus A16(CA16) is one of the major causes of hand, foot, and mouth disease(HFMD) worldwide, which is a common illness that affects children. The frequent occurrence of HFMD outbreaks has become a serious public health problem in Asia. Therefore, it is important to understand the pathogenesis and replication of CA16. In this study, a stable infectious c DNA clone of an epidemic strain of Coxsackievirus A16(CA16) was assembled, and subsequently a reporter virus(e GFP-CA16) was constructed by inserting the e GFP gene between the 5'-UTR and the N-terminus of VP4, with the addition of a 2A protease cleavage site(ITTLG) at its C-terminus. This was transfected into Vero cells to generate infectious recombinant viruses. The growth characteristics and plaque morphology, in vitro, in mammalian cells were found to be indistinguishable between the parental and recombinant viruses. Although the e GFP-CA16 showed smaller plaque size as compared to recombinant CA16, both were found to exhibit similar growth trends and EC50 of NITD008. In summary, this stable infectious c DNA clone should provide a valuable experimental system to study CA16 infection and host response. The e GFP-CA16 is expected to provide a powerful tool to monitor e GFP expression in infected cells and to evaluate the antiviral activity of potential antiviral agents in the treatment of CA16 infections. | Chenglin Deng Xiaodan Li Siqing Liu Linlin Xu Hanqing Ye Cheng-Feng Qin Bo Zhang | 2015 | Virologica Sinica2015，30，4： | 5 |
| 3 | Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study显示文摘Omsk hemorrhagic fever virus(OHFV) is a tick-borne flavivirus classified as a biosafety level-4(BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion(OHFVDNS1) and reporter virus replacing NS1 with the Gaussia luciferase(Gluc)(OHFV-ΔNS1-Gluc). Both the defective OHFVDNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1(BHK21NS1) but not in na?ve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z0 value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories. | Qiuyan Zhang Na Li Chenglin Deng Zherui Zhang Xiaodan Li Kentaro Yoshii Hanqing Ye Bo Zhang | 2019 | Virologica Sinica2019，34，4： | 4 |
| 4 | p53-dependent upregulation of PIG3 transcription by γ-ray irradiation and its interaction with KAP1 in responding to DNA damage显示文摘PIG3 (p53-inducible gene 3), originally identified as one of a set of genes induced by p53 before the onset of apoptosis, was assumed to contribute to early cellular response to DNA damage. Here, we studied the relation between p53 status and the increased expression of PIG3 by ionizing radiation (IR), and the related clues regarding the involvement of PIG3 in the cellular response to IR-induced DNA damage signaling. We demonstrated that the pentanucleotide microsatellite sequence was responsible for the p53-dependent induction of PIG3 transcription after irradiation, while sequence upstream of PIG3 promoter could maintain the basal level of expression which was not inducible by irradiation. The interaction of PIG3 and the KRAB-ZFP-associated protein 1 (KAP1), a DNA damage response protein, was revealed. PIG3 nucleus foci were formed 15 min after γ-ray irradiation, and which were found to partially colocalize with the phospho-KAP-1 foci as well as γ-H2AX foci. Although the lac operator tagged EGFP based reporter system revealed that PIG3 does not remodel chromatin in large scale in the cells under normal growing condition, it indeed prompted the chromatin relaxation in the cellular response to DNA damage signaling. All these data suggest that PIG3 is involved in IR-induced DNA damage response, and which maybe partially attribute to its interaction with KAP1. | QIN Xia ZHANG ShiMeng LI Bingi LIU XiaoDan HE XingPeng SHANG ZengFu XU QinZhi ZHAO ZengQiang YE QiNong ZHOU PingKun | 2011 | Chinese Science Bulletin2011，56，30： | 2 |
| 5 | Ultrastructural characterization of avian influenza A(H7N9) virus infecting humans in China显示文摘Dear Editor,In March 2013,the first 3 cases of severe disease dueto a novel avian-origin influenza A(H7N9)virus weredetected in the Chinese provinces of Shanghai and Anhui(Gao R,et al.,2013).A total of 339 laboratory-confirmedcases with 100 deaths were reported until January 142014(WHO,2014).To the best of our knowledge,thisis the first time that human infection with the avian in-fluenza A H7N9 subtype has been detected.Prior to | Rongbao Gao Jingdong Song Ye Zhang Shumei Zou Tian Bai Xiaodan Li Jianguo Qu Jianfang Zhou Tao Hung Yuelong Shu | 2014 | Virologica Sinica2014，29，2： | 2 |
| 6 | Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study显示文摘 | HaiYing Chen Hui Yuan Rongbao Gao Jinxiang Zhang Dayan Wang Ying Xiong GuoYin Fan Fan Yang Xiaodan Li Jianfang Zhou Shumei Zou Lei Yang Tao Chen Libo Dong Hong Bo Xiang Zhao Ye Zhang Yu Lan Tian Bai Jie Dong Qun Li ShiWen Wang YanPing Zhang Hui Li Tian Go | 2014 | The Lancet2014，，： | 1 |
| 7 | Genesis and Evolution of the Leimengou Porphyry Mo Deposit in West Henan Province, East Qinling- Dabie Belt, China: Constraints from Hydrothermal Alteration, Fluid Inclusions and Stable Isotope Data显示文摘 | Chen Xiaodan Ye Huishou Wang Huan | 2014 | Journal of Asian Earth Sciences2014，79，： | 1 |
| 8 | Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase显示文摘We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV. | Chao Shan Xiaodan Li Chenglin Deng Baodi Shang Linlin Xu Hanqing Ye Zhiming Yuan Bo Zhang | 2013 | Virologica Sinica2013，28，3： | 1 |
| 9 | Perfluorooctyl bromide nanoemulsions holding MnO_(2) nanoparticles with dual-modality imaging and glutathione depletion enhanced HIFU-eliciting tumor immunogenic cell death显示文摘Tumor-targeted immunotherapy is a remarkable breakthrough,offering the inimitable advantage of specific tumoricidal effects with reduced immune-associated cytotoxicity.However,existing platforms suffer from low efficacy,inability to induce strong immunogenic cell death(ICD),and restrained capacity of transforming immune-deserted tumors into immune-cultivated ones.Here,an innovative platform,perfluorooctyl bromide(PFOB)nanoemulsions holding MnO_(2) nanoparticles(MBP),was developed to orchestrate cancer immunotherapy,serving as a theranostic nanoagent for MRI/CT dual-modality imaging and advanced ICD.By simultaneously depleting the GSH and eliciting the ICD effect via highintensity focused ultrasound(HIFU)therapy,the MBP nanomedicine can regulate the tumor immune microenvironment by inducing maturation of dendritic cells(DCs)and facilitating the activation of CD8^(+)and CD4^(+)T cells.The synergistic GSH depletion and HIFU ablation also amplify the inhibition of tumor growth and lung metastasis.Together,these findings inaugurate a new strategy of tumor-targeted immunotherapy,realizing a novel therapeutics paradigm with great clinical significance. | Xinping Kuai Yuefei Zhu Zheng Yuan Shengyu Wang Lin Lin Xiaodan Ye Yiping Lu Yu Luo Zhiqing Pang Daoying Geng Bo Yin | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 1 |
| 10 | Crosstalk between PC 12 cells and endothelial cells in an artificial neurovascular niche constructed by a dual-functionalized selfassembling peptide nanofiber hydrogel显示文摘The coordination between neurogenesis and angiogenesis plays an important role in nerve tissue development and regeneration.Recently,using bioactive materials to drive neurogenic and angiogenic responses has gained increasing attention.Understanding the neurovascular link between regulatory cues offers valuable insight into the mechanisms underlying nerve regeneration and the design of new bioactive materials.In this study,we utilized a dual-functionalized peptide nanofiber hydrogel presenting the brain-derived neurotrophic factor and vascular endothelial growth factor mimetic peptides RGIDKRHWNSQ(RGI)and KLTWQELYQLKYKGI(KLT)to construct an artificial neurovascular microenvironment.The dual-functionalized peptide nanofiber hydrogel enhanced the neurite outgrowth of pheochromocytoma(PC12)cells and tube-like structures formation of human umbilical vein endothelial cells(HUVECs)in vitro,and promoted rapid lesion infiltration of neural and vascular cells in a rat brain injury model.Using indirect co-culture models,we found that the dual-functionalized peptide hydrogel effectively mediated neurovascular crosstalk by regulating secretion of paracrine factors from PC12 cells and HUVECs.When the two cells types were directly co-cultured on the dua卜functionalized peptide hydrogel,the efficiency of cell-cell communication was enhanced,which further accelerated the differentiation and maturation of PC12 cells with an increased number of pseudopodia and spread morphology,and HUVECs tube-like structure formation.In summary,the dual-functionalized peptide nanofiber hydrogel successfully formed an artificial neurovascular niche to directly regulate the behaviors of neural and vascular cells and promote their neurovascular crosstalk through paracrine signaling and direct cell-cell contact. | Zhe Zhang Yi Chai He Zhao Shuhui Yang Wei Liu Zihui Yang Weilong Ye Chenlong Wang Xiaohan Gao Xiangdong Kong Xiaodan Sun Lingyun Zhao Tuoyu Chen Yuqi Zhang Jiaju Lu Xiumei Wang | 2022 | Nano Research2022，15，2： | 0 |
| 11 | An innovative three-layer strategy in response to a quartan malaria outbreak among forest goers in Hainan Island,China:a retrospective study显示文摘Background:An outbreak of Plasmodium malariae infection among forest goers in Sanya City of Hainan Island,China was reported in 2015.In response to this outbreak,an innovative three-layer strategy(TLS)targeted forest goers was adapted based on the 1-3-7 approach.Main text:Key elements of TLS are:(i)The village with fve malaria cases and adjacent villages were set as the frst layer.All residents including forest goers were taken as the high-risk population(HRP).Active case detection(ACD)by blood smear microscopy and PCR was selected as the primary measure,and passive case detection(PCD)as complementary measure.One case was identifed under TLS implementation.(ii)The township with cases(Gaofeng Town)and the nearby towns were chosen as the second layer.Only forest goers were screened by ACD,while PCD as a routine screening method.7831 blood smears collected by ACD and PCD and tested with negative results.(iii)The city with cases(Sanya City)and others 12 counties/county-level cities were selected as the third layer.Malaria cases were monitored passively.A total of 77,555 blood slides were screened by PCD with zero positive sample.For each layer,the malaria vector mosquitoes were monitored using light traps,cattle-baited/human-bait traps.Anopheles minimus(dominant species),An.sinensis and An.dirus were captured.Vector control measures mainly include insecticide residual spraying and long-lasting insecticide nets.The capacity of clinicians,public health practitioners and laboratory technicians has been improved through training.During 2016‒2018,TLS and chemoprophylaxis were implemented in the same areas.In the frst layer,all residents were monitored by ACD,and malaria chemoprophylaxis were distributed,89.5%of forest goers were using chemoprophylaxis against malaria.The blood smears(3126 by ACD plus 1516 by PCD)were with zero positive results.Chemoprophylaxis and ACD were ofered to forest goers once a year,and PCD in residents as a complementary measure in the second and third layer,77.8%and 95.1%of forest goers received chemoprophylaxis.In each layer,vector surveillance and control of malaria and trainings for medical staf Conclusions:TLS was efective in blocking the outbreak by P.malariae among forest goers in Hainan in malaria elimination stage.However,whether it could prevent the malaria resurgence in the post-elimination phase needs to be further assessed. | Yuchun Li Yingjuan Huang Renqiang Chen Weizhen Huang Huanzhi Xu Rongshen Ye Shaoling Huang Ji Zhen Xiaodan Wen Guoyi Wang Yong Liu Haishan Li Zaichun Zheng Jian Wang Guoshen Wang Chong Chen Wen Zeng Feng Meng Xiaoming Huang Guangze Wang Bing Yang Yan Chen | 2022 | Infectious Diseases of Poverty2022，11，5： | 0 |
| 12 | A method for distinguishing benign and malignant pulmonary nodules based on 3D dual path network aided by K-means clustering analysis显示文摘In the USA,there were about 1806590 new cancer cases in 2020,and 606520 cancer deaths are expected to have occurred in 2021.Lung cancer has become the leading cause of death from cancer in both men and women(Siegel et al.,2020).Clinical studies show that the five-year survival rate of lung cancer patients after early diagnosis and treatment intervention can reach 80%,compared with that of patients having advanced lung cancer.Thus,the early diagnosis of lung cancer is a key factorto reduce mortality. | Dachuan GAO Xiaodan YE Xuewen HOU Yang CHEN Xue KONG Yuanzhong XIE Shengdong NIE | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，11： | 0 |
| 13 | Oridonin inhibits SARS-CoV-2 replication by targeting viral proteinase and polymerase显示文摘COVID-19 has become a global public health crisis since its outbreak in China in December 2019.Currently there are few clinically effective drugs to combat SARS-CoV-2 infection.The main protein(M^(pro)),papain-like protease(PL^(pro))and RNA-dependent RNA polymerase(RdRp)of SARS-CoV-2 are involved in the viral replication,and might be prospective targets for anti-coronavirus drug development.Here,we investigated the antiviral activity of oridonin,a natural small-molecule compound,against SARS-CoV-2 infection in vitro.The time-of-addition analysis showed that oridonin efficiently inhibited SARS-CoV-2 infection by interfering with the genome replication at the post-entry stage.Mechanistically,the inhibition of viral replication by oridonin depends on the oxidation activity ofα,β-unsaturated carbonyl.Further experiments showed that oridonin not only effectively inhibited SARS-CoV-2 Mpro activity,but also had some inhibitory effects on PLpro-mediated deubiquitinating and viral polymerase-catalyzed RNA elongation activities at high concentrations.In particular,oridonin could inhibit the bat SARS-like CoV and the newly emerged SARS-CoV-2 omicron variants(BA.1 and BA.2),which highlights its potential as a pan-coronavirus antiviral agent.Overall,our data provide strong evidence that oridonin is an efficient antiviral agent against SARS-CoV-2 infection. | Zherui Zhang Hongqing Zhang Yanan Zhang Qiuyan Zhang Qiaojie Liu Yanyan Hu Xiaoling Chen Jing Wang Yujia Shi Chenglin Deng Peng Gong Bo Zhang Xiaodan Li Bing Zhu Hanqing Ye | 2023 | Virologica Sinica2023，38，3： | 0 |
| 14 | Response of nitrogen fractions in the rhizosphere and bulk soil to organic mulching in an urban forest plantation显示文摘Nitrogen is an essential component in forest ecosystem nutrient cycling.Nitrogen fractions,such as dissolved nitrogen,ammonium,nitrate,and microbial biomass nitrogen,are sensitive indicators of soil nitrogen pools which affect soil fertility and nutrient cycling.However,the responses of nitrogen fractions in forest soils to organic mulching are less well understood.The rhizosphere is an important micro-region that must be considered to better understand element cycling between plants and the soil.A field investigation was carried out on the effect of mulching soil in a 15-year-old Ligustrum lucidum urban plantation.Changes in total nitrogen and nitrogen fractions in rhizosphere and bulk soil in the topsoil(upper 20 cm)and in the subsoil(20-40 cm)were evaluated following different levels of mulching,in addition to nitrogen contents in fine roots,leaves,and organic mulch.The relationships between nitrogen fractions and other measured variables were analysed.Organic mulching had no significant effect on most nitrogen fractions except for the rhizosphere microbial biomass nitrogen(MBN),and the thinnest(5 cm)mulching layer showed greater effects than other treatments.Rhizosphere MBN was more sensitive to mulching compared to bulk soil,and was more affected by soil environmental changes.Season and soil depth had more pronounced effects on nitrogen fractions than mulching.Total nitrogen and dissolved nitrogen were correlated to soil phosphorus,whereas other nitrogen fractions were strongly affected by soil physical properties(temperature,water content,bulk density).Mulching also decreased leaf nitrogen content,which was more related to soil nitrogen fractions(except for MBN)than nitrogen contents in either fine roots or organic mulch.Frequent applications of small quantities of organic mulch contribute to nitrogen transformation and utilization in urban forests. | Xiaodan Sun Gang Wang Yuqian Ye Qingxu Ma Qingwei Guan Davey L.Jones | 2021 | Journal of Forestry Research2021，32，6： | 0 |
| 15 | The Impact of a Health Forecasting Service on the Visits and Costs in Outpatient and Emergency Departments for COPD Patients--Shanghai Municipality,China,October 2019–April 2020显示文摘What is already known on this topic?The morbidity and mortality of chronic obstructive pulmonary disease(COPD)is associated with adverse weather and air pollution.However,COPD patients are not able to be alerted in advance of high risk environments.What is added by this report?This prospective controlled trial conducted in Pudong New Area of Shanghai from October 2019 to April 2020 provided evidence of COPD risk forecasting service on the reductions in visits and costs of COPD patients in outpatient and emergency departments in China for the first time.What are the implications for public health practice?This study suggests that COPD risk forecasting service could be integrated into existing COPD management in public health to improve the health and economic outcomes. | Xiaofang Ye Zhitao Li Xin Zhou Xiaonan Ruan Tao Lin Ji Zhou Dandan Yang Sixu Yang Xiaodan Chen Kang Wu Xiaonan Wang Juzhong Ke Xiaolin Liu Li Peng Li Luo | 2021 | China CDC weekly2021，3，23： | 0 |
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