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| 1 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 2 | Effect of the Cutter Parameters and Machining Parameters on the Interference in Gear Slicing显示文摘Current researches have not yet found the effect law of the cutter parameters and machining parameters on the interference in gear slicing,the interference between the cutter and machined gear often happens because the appropriate cutter parameters and machining parameters cannot be set,which reduces the gear machining accuracy.The relative position between the major flank face and edge-sweeping surface,distribution law of the interference area in forming process of edge-sweeping surface,and effect law of relative positions among edge-sweeping surfaces on the interference are studied by graphical analysis.The effect law of the cutter parameters and machining parameters on the interference is found.The effect law shows that the interference in gear slicing can be controlled when the relief angle measured on the top edge and feed of every rotation are chosen respectively larger than 9°and smaller than 0.15 mm/r.An internal helical gear is sliced with the spur slice cutter and the cutter parameters and machining parameters are set based on above the effect law.The machined gear is measured in Gear Measuring Center and the detection result shows that the comprehensive accuracy reaches GB/T Class 7,where some reach GB/T Class 6.The result can meet the gear machining accuracy requirement and shows that the effect law found is valid.The problem of the interference in gear slicing is solved and the gear machining accuracy can be improved. | CHEN Xinchun LI Jia LOU Benchao SHI Jiang YANG Qijun | 2013 | Chinese Journal of Mechanical Engineering2013，26，6： | 10 |
| 3 | In vivo evaluation of renal function using diffusion weighted imaging and diffusion tensor imaging in type 2 diabetics with normoalbuminuria versus microalbuminuria显示文摘这个工作试图估计散开的价值加权的成像(DWI ) 和在有希望地与 normoalbuminuria (NAU ) 对 microalbuminuria (MAU ) 在类型 2 糖尿病的病人检测早阶段的肾损害的散开张肌成像(DTI ) 。有正常的肾功能的 30 个 T2DM 病人的一个总数被招募并且分到 NAU 组(n = 14 ) 或 MAU 组(n = 16 ) 根据 8 h,一夜间尿的蛋白尿排泄率(AER ) 结果。健康检查接受者的一个当代的队作为控制被包括(n = 12 ) 。DWI 和 DTI 扫描用 SE 单个射击的 EPI,和明显的散开系数(模数转换器) 在双边的肾上被执行,肾的实质的部分 anisotropy (FA ) 从模数转换器和三个组的 FA 地图是坚定的。模数转换器和 FA 价值在三个组之中被比较。根据有 400 s/mm 的 b 价值的 DWI <啜class=“ a-plus-plus ”> MAU 和 NAU 组显著地显示出的 2 ,与健康控制相比降低了吝啬的模数转换器价值( P 3 公里<啜class=“ a-plus-plus ”> 2 /s]比的稍微低 NAU 组[( 2.31 b >水平枌?枌?? | Xiaoyan Chen Wenxia Xiao Xinchun Li Jianxun He Xiaochun Huang Yuyu Tan | 2014 | Frontiers of Medicine2014，8，4： | 10 |
| 4 | Modified graphene as novel lubricating additive with high dispersion stability in oil显示文摘Graphene is a promising material as a lubricant additive for reducing friction and wear.Here,a dispersing method which combines chemical modification of graphene by octadecylamine and dicyclohexylcarbodiimide with a kind of effective dispersant has been successfully developed to achieve the remarkable dispersion stability of graphene in base oil.The stable dispersion time of modified graphene(0.5 wt%)with dispersant(1 wt%)in PAO-6 could be up to about 120 days,which was the longest time reported so far.At the same time,the lubricant exhibits a significant improvement of tribological performance for a steel ball to plate tribo-system with a normal load of 2 N.The coefficient of friction between sliding surfaces was~0.10 and the depth of wear track on plate was~21 nm,which decreased by about 44%and 90%when compared to pure PAO-6,respectively.Furthermore,the analysis of the lubricating mechanisms in regard to the sliding-induced formation of nanostructured tribo-film has been contacted by using Raman spectra and TEM. | Pu WU Xinchun CHEN Chenhui ZHANG Jiping ZHANG Jianbin LUO Jiyang ZHANG | 2021 | Friction2021，9，1： | 9 |
| 5 | Th1 cytokines, true functional signatures for protective immunity against TB?显示文摘The lack of an effective preventative vaccine against tuberculosis(TB)presents a great challenge to TB control.Since it takes an extremely long time to accurately determine the protective efficacy of TB vaccines,there is a great need to identify the surrogate signatures of protection to facilitate vaccine development.Unfortunately,antigen-specific Th1 cytokines that are currently used to evaluate the protective efficacy of the TB vaccine,do not align with the protection and failure of TB vaccine candidates in clinical trials.In this review,we discuss the limitation of current Th1 cytokines as surrogates of protection and address the potential elements that should be considered to finalize the true functional signatures of protective immunity against TB. | Gucheng Zeng Guoliang Zhang Xinchun Chen | 2018 | Cellular & Molecular Immunology2018，15，3： | 6 |
| 6 | Co-infections of SARS-CoV-2 with multiple common respiratory pathogens in infected patients显示文摘Dear Editor,In late December 2019,an outbreak of severe pneumonia caused by an unknown etiological agent was initially reported in Wuhan,China,and then quickly spread throughout China and even abroad(Zhu et al.,2020).Swift control measures and etiological investigations were conducted by the Chinese government,physicians and researchers,and by using high throughput sequencing and virological testing,the mysterious pneumonia pathogen was shown to be a novel coronavirus(SARS-CoV-2)capable of person-to-person transmission(Zhu et al.,2020).Coronaviruses are a group of non-segmented,enveloped and positive-sense RNA viruses that belong to the subfamily of Coronavirinae in the family of Coronavirdiae and order of Nidovirales. | Dachuan Lin Lei Liu Mingxia Zhang Yunlong Hu Qianting Yang Jiubiao Guo Yongchao Guo Youchao Dai Yuzhong Xu Yi Cai Xinchun Chen Zheng Zhang Kaisong Huang | 2020 | Science China(Life Sciences)2020，63，4： | 6 |
| 7 | The association between metabolic abnormality and endometrial cancer: A large case-control study in China显示文摘 | Yan Zhang Zhiwei Liu Xinchun Yu Xiaofei Zhang Shiming Lü Xiaoduan Chen Bingjian Lü | 2010 | Gynecologic Oncology2010，，1： | 3 |
| 8 | Monodispersed Pt nanoparticles on reduced graphene oxide by a non-noble metal sacrificial approach for hydrolytic dehydrogenation of ammonia borane显示文摘 | Yao Chen Xinchun Yang Mitsunori Kitta Qiang Xu | 2017 | Nano Research2017，10，11： | 2 |
| 9 | Influence of tribofilm on superlubricity of highly-hydrogenated amorphous carbon films in inert gaseous environments显示文摘In this study,we mainly focus on the structural morphology and inter-atomic bonding state of tribofilms resulting from a highly-hydrogenated amorphous carbon(a-C:H) film in order to ascertain the underlying mechanisms for its superlubric behavior(i.e.,less than 0.01 friction coefficient).Specifically,we achieved superlubricity(i.e.,friction coefficients of down to 0.003) with this film in dry nitrogen and argon atmospheres especially when the tribo-pair is made of an a-C:H coated Si disk sliding against an a-C:H coated steel ball,while the a-C:H coated disk against uncoated ball does not provide superlubricity.We also found that the state of superlubricity is more stable in argon than in nitrogen and the formation of a smooth and uniformly-thick carbonaceous tribofilm appears to be one of the key factors for the realization of such superlubricity.Besides,the interfacial morphology of sliding test pairs and the atomic-scale bond structure of the carbon-based tribofilms also play an important role in the observed superlubric behavior of a-C:H films.Using Raman spectroscopy and high resolution transmission electron microscopy,we have compared the structural differences of the tribofilms produced on bare and a-C:H coated steel balls.For the a-C:H coated ball as mating material which provided superlow friction in argon,structural morphology of the tribofilm was similar or comparable to that of the original a-C:H coating;while for the bare steel ball,the sp^2-bonded C fraction in the tribofilm increased and a fingerprint-like nanocrystalline structure was detected by high resolution transmission electron microscopy(HRTEM).We also calculated the shear stresses for different tribofilms,and established a relationship between the magnitude of the shear stresses and the extent of sp^3-sp^2 phase transformation. | LIU ShuWei ZHANG ChenHui OSMAN Eryilmaz CHEN XinChun MA TianBao HU YanZhong LUO JianBin ALI Erdemir | 2016 | Science China(Technological Sciences)2016，59，12： | 2 |
| 10 | Modified ADM1 for modeling free ammonia inhibition in anaerobic acidogenic fermentation with high-solid sludge显示文摘Anaerobic acidogenic fermentation with high-solid sludge is a promising method for volatile fatty acid(VFA) production to realize resource recovery. In this study, to model inhibition by free ammonia in high-solid sludge fermentation, the anaerobic digestion model No. 1(ADM1) was modified to simulate the VFA generation in batch, semicontinuous and full scale sludge. The ADM1 was operated on the platform AQUASIM 2.0.Three kinds of inhibition forms, e.g., simple inhibition, Monod and non-inhibition forms,were integrated into the ADM1 and tested with the real experimental data for batch and semi-continuous fermentation, respectively. The improved particle swarm optimization technique was used for kinetic parameter estimation using the software MATLAB 7.0. In the modified ADM1, the Ksof acetate is 0.025, the km,acis 12.51, and the KI_NH3is 0.02,respectively. The results showed that the simple inhibition model could simulate the VFA generation accurately while the Monod model was the better inhibition kinetics form in semi-continuous fermentation at p H 10.0. Finally, the modified ADM1 could successfully describe the VFA generation and ammonia accumulation in a 30 m3full-scale sludge fermentation reactor, indicating that the developed model can be applicable in high-solid sludge anaerobic fermentation. | Jie Bai He Liu Bo Yin Huijun Ma Xinchun Chen | 2017 | Journal of Environmental Sciences2017，29，2： | 1 |
| 11 | A novel solid state fault current limiter for DC power distribution network显示文摘 | LUO Fang CHEN Jian LIN Xinchun | 2008 | IEEE Trans on Power Electronics2008，9，5： | 1 |
| 12 | Diagnosis of Ac- tive Tuberculosis in China Using an In-House Gamma Interferon En- zyme-Linked Immunospot Assay显示文摘 | Xinchun Chen Qianting :ang Mingxia Zhang | 2009 | Clin Vaccine Immunol2009，16，16： | 1 |
| 13 | Effects of Long-Term Fertilization Strategies on Soil Productivity and Soybean Rhizobial Diversity in a Chinese Mollisol显示文摘Rhizobial diversity is affected by interactions between soil features, fertilization strategy, and cropping system. However, interactions among the rhizobial community, chemical-organic manure fertilization, and plant production have not been well documented in Mollisols from long-term experiments. Aimed at maintaining and recovering the productivity of Chinese Mollisols, a long-term fertilization experiment had been carried out for 29 years under a wheat-maize-soybean rotation system, involving the application of recycled organic manure (ROM), chemical fertilizers (N, P, and/or K), or ROM plus N, P, and/or K. In the present study, the effects of different treatments were evaluated by determining soil physicochemical features, soybean production, and soybean rhizobial diversity. The results showed that application of ROM plus NPK maintained or increased soil fertility, which was accompanied by higher production and higher diversity of rhizobia, as compared with the other treatments. The negative association of Bradyrhizobium japonicum with N fertilizer, positive association of B. diazoefficiens with soil pH, and alleviation of N-inhibition on the diversity of Bradyrhizobium by the addition of ROM were recorded as new findings. Therefore, application of ROM or ROM plus NPK could be a feasible strategy for maintaining and recovering the fertility of Chinese Mollisols, whereas rhizobial diversity could be an indicator of soil fertility. | YAN Jun HAN Xiaozeng CHEN Xu LU Xinchun CHEN Wenfeng WANG Entao ZOU Wenxiu ZHANG Zhiming | 2019 | Pedosphere2019，29，6： | 1 |
| 14 | Tumor-derived CD4 + CD25 + regulatory T cell suppression of dendritic cell function involves TGF- β and IL-10显示文摘 | Nicolas Larmonier Marilyn Marron Yi Zeng Jessica Cantrell Angela Romanoski Marjan Sepassi Sylvia Thompson Xinchun Chen Samita Andreansky Emmanuel Katsanis | 2006 | Cancer Immunology, Immunotherapy2006，，1： | 1 |
| 15 | Association between plasma homocysteine levels and obstructive sleep apnoea in patients with ischaemic stroke显示文摘 | Maogang Chen Bona Wu Xinchun Ye Zhiming Zhou Xuanye Yue Qizhang Wang Wanxiang Wang Yongjun Jiang Xuegan Lian Zhaoyao Chen Gelin Xu Xinfeng Liu | 2011 | Journal of Clinical Neuroscience2011，，11： | 1 |
| 16 | Recombinant hepatitis B core antigen carrying preS1 epitopes induce immune response against chronic HBV infection显示文摘 | Xinchun Chen Meizhong Li Xiaohua Le | 2004 | Vaccine2004，22，： | 1 |
| 17 | Y_(2)O_(3) nanosheets as slurry abrasives for chemical-mechanical planarization of copper显示文摘Continued reduction in feature dimension in integrated circuits demands high degree of flatness after chemical mechanical polishing.Here we report using new yttrium oxide(Y_(2)O_(3))nanosheets as slurry abrasives for chemical-mechanical planarization(CMP)of copper.Results showed that the global planarization was improved by 30%using a slurry containing Y_(2)O_(3) nanosheets in comparison with a standard industrial slurry.During CMP,the two-dimensional square shaped Y_(2)O_(3) nanosheet is believed to induce the low friction,the better rheological performance,and the laminar flow leading to the decrease in the within-wafer-non-uniformity,surface roughness,as well as dishing.The application of the two-dimensional nanosheets as abrasive in CMP would increase the manufacturing yield of integrated circuits. | Xingliang HE Yunyun CHEN Huijia ZHAO Haoming SUN Xinchun LU Hong LIANG | 2013 | Friction2013，1，4： | 1 |
| 18 | Recombinant hepatitis B core antigen carrying pre S1 epitopes induce immune response against chronic HBV infection显示文摘 | Chen Xinchun Li Meizhong Le Xiaohua | 2004 | Vaccine2004，22，： | 1 |
| 19 | Tumor-derived CD4+CD25+ regulatory T cell suppression of dendritic cell function involves TGF-β and IL-10显示文摘 | Nicolas Larmonier Marilyn Marron Yi Zeng Jessica Cantrell Angela Romanoski Marjan Sepassi Sylvia Thompson Xinchun Chen Samita Andreansky Emmanuel Katsanis | 2007 | Cancer Immunology Immunotherapy2007，，1： | 1 |
| 20 | Covert communication with beamforming over MISO channels in the finite blocklength regime显示文摘This paper considers beamforming techniques for covert communication over multiple input single output(MISO) channels with finite blocklength. We first show that the optimal input distribution for covert communication over complex Gaussian channels is circular symmetric complex Gaussian. By reviewing our previous results on the throughput of Gaussian random coding over additive white Gaussian noise(AWGN) channels, the achievability and converse bounds on the attainable throughput over MISO channels are analyzed. Then, the optimal beamforming strategies and their relationship with the transmitting power are thoroughly investigated in a variety of situations in terms of several types of channel state information available at both ends of the system. We reveal the fact that the maximal allowable transmit power is not constrained by covertness requirement when there is full channel information of the adversary, while there is an upper bound of the transmit power which is based on beamforming and an outage-based covertness criterion if there is only partial channel information of the adversary. Finally, numerical results are presented to show that the throughput of covert communication can be increased notably by adopting a proper beamforming strategy in MISO channels in comparison with the single antenna case. | Xinchun YU Yuan LUO Wen CHEN | 2021 | Science China(Information Sciences)2021，64，9： | 1 |
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