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| 1 | Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis显示文摘在哺乳动物的精子发生被 piRNA 表示的二个波浪描绘:一个人对应于负责的经典 piRNAs 因为 silencing retrotransponsons 和第二个波浪主要在 pachytene spermatocytes 从 nontransposon intergenic 区域被导出,但是这些 pachytene piRNAs 的功能大部分是未知的。这里,我们在在老鼠伸长指示巨大的 mRNA 消除报导 pachytene piRNAs 的参与 spermatids (ES ) 。我们证明包含鼠科的 PIWI (MIWI ) 和 deadenylase CAF1 的导致 piRNA 的 silencing 建筑群(pi-RISC ) 有选择地在 ES 被装配,它负责经由在体的房间类似于 miRNAs 的行动的机制导致 mRNA deadenylation 和腐烂。如此的一个高度安排的节目看起来充分利用 pachytene piRNAs 的能力从 nontransposon intergenic 区域导出的多样化的指向的庞大的全部剧目。这些调查结果建议 pachytene piRNAs 负责为精子生产作准备,从 ES 使广阔细胞的节目失去活性。 | Lan-Tao Gou Peng Dai Jian-Hua Yang Yuanchao Xue Yun-Ping Hu Yu Zhou Jun-Yan Kang Xin Wang Hairi Li Min-Min Hua Shuang Zhao Si-Da Hu Li-Gang Wu Hui-Juan Shi Yong Li Xiang-Dong Fu Liang-Hu Qu En-Duo Wang Mo-Fang Liu | 2014 | Cell Research2014，24，6： | 39 |
| 2 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 3 | Infection with novel coronavirus(SARS-CoV-2)causes pneumonia in Rhesus macaques显示文摘The 2019 novel coronavirus(SARS-CoV-2)outbreak is a major challenge for public health.SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases,with a mortality rate of^6.4%worldwide(based on World Health Organization daily situation report).However,the dynamics of viral infection,replication and shedding are poorly understood.Here,we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2.After intratracheal inoculation,the first peak of viral RNA was observed in oropharyngeal swabs one day post infection(1 d.p.i.),mainly from the input of the inoculation,while the second peak occurred at 5 d.p.i.,which reflected on-site replication in the respiratory tract.Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals,characterized by hyperemia and edema,and infiltration of monocytes and lymphocytes in alveoli.We also identified SARS-CoV-2 RNA in respiratory tract tissues,including trachea,bronchus and lung;and viruses were also re-isolated from oropharyngeal swabs,bronchus and lung,respectively.Furthermore,we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2.The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics. | Chao Shan Yan-Feng Yao Xing-Lou Yang Yi-Wu Zhou Ge Gao Yun Peng Lian Yang Xue Hu Jin Xiong Ren-Di Jiang Hua-Jun Zhang Xiao-Xiao Gao Cheng Peng Juan Min Ying Chen Hao-Rui Si Jia Wu Peng Zhou Yan-Yi Wang Hong-Ping Wei Wei Pang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Zheng-Li Shi Zhi-Ming Yuan | 2020 | Cell Research2020，30，8： | 19 |
| 4 | Rapid Detection and Identification of Infectious Pathogens Based on High-throughput Sequencing显示文摘 | Pei-Xiang Ni Xin Ding Yin-Xin Zhang Xue Yao Rui-Xue Sun Peng Wang Yan-Ping Gong Jia-Li Zhou Dong-Fang Li Hong-Long WO Xin Yi Ling Yang Yun Long | 2015 | Chinese Medical Journal2015，，7： | 13 |
| 5 | 重复经颅磁刺激治疗抑郁症的机制研究进展(英文)显示文摘概述:抑郁障碍是目前最常见的心理健康问题之一。然而,对于该疾病的基于机制的治疗方法仍然是难以捉摸的。重复经颅磁刺激(rTMS)是一种非侵入性的程序,它可以以一种脉冲磁场来刺激大脑中的电活动,被认为是一种有效的抑郁症治疗方式。在这里,我们回顾了关于rTMS治疗抑郁症的临床和基础研究的主要发现,包括它的抗抑郁疗效,基本原理,调节神经回路、神经递质和脑网络、海马神经发生、和突出的功效,以及分子通路。 | Zhengwu PENG Cuihong ZHOU Shanshan XUE Jie BAI Shoufen YU Xiaosa LI Huaning WANG Qingrong TAN | 2018 | 上海精神医学2018，30，2： | 13 |
| 6 | Nutritional Composition and Assessment of Gracilaria lemaneiformis Bory显示文摘化学作文,矿物质元素,维生素,免费的丰满的酸和氨基酸可食的红水藻 Gracilaria lemaneiformis Bory 满足,在 Nan'ao 岛附近的海成年,广东省,在现在的学习被分析。Gracilaria lemaneiformis Bory 显示出 14.65% 的一个全部的糖内容。蛋白质内容是 21% ,哪个约 41% 决心是必要氨基酸(EAA ) 。主要氨基酸部件是 glutamic 酸,白氨酸,精氨酸,和丙氨酸。EAA assayed,蛋氨酸和半胱氨酸看起来与食物和联合国的农业组织提供的 EAA 模式相比是大多数限制氨基酸。全部的类脂化合物内容是 0.87% 并且包括了不饱和的丰满的酸(61%) 的一篇高作文,主要当罂酸的酸和油的酸,和一些多元不堡和总计丰满的酸;十六酸酸是浸透的酸的主要部件(39%) 。维生素 C,碘,磷,和锌相对高级也是在 G 的现在。lemaneiformis。在 G 之间的营养的作文。lemaneiformis 和 Nostoc flagelliforme,广泛地在中国社会被吃的稀罕水藻,被比较。结果建议那 N。flagelliforme 能被用代替由 G。lemaneiformis,不是仅仅因为他们的类似的形状,而且因为他们的近似营养的作文。Gracilaria lemaneiformis 以后可以可能在人的食谱用作潜在的健康食物。 | Xue Wen Changlian Peng Houcheng Zhou Zhifang Lin Guizhu Lin Shaowei Chen Ping Li | 2006 | Journal of Integrative Plant Biology2006，48，9： | 13 |
| 7 | On the Key Dynamical Processes Supporting the 21.7 Zhengzhou Record-breaking Hourly Rainfall in China显示文摘An extremely heavy rainfall event occurred in Zhengzhou,China,on 20 July 2021 and produced an hourly rainfall rate of 201.9 mm,which broke the station record for China's Mainland.Based on radar observations and a convection-permitting simulation using the WRF-ARW model,this paper investigates the multiscale processes,especially those at the mesoscale,that support the extreme observed hourly rainfall.Results show that the extreme rainfall occurred in an environment characteristic of warm-sector heavy rainfall,with abundant warm moist air transported from the ocean by an abnormally northward-displaced western Pacific subtropical high and Typhoon In-Fa(2021).However,rather than through back building and echo training of convective cells often found in warm-sector heavy rainfall events,this extreme hourly rainfall event was caused by a single,quasi-stationary storm in Zhengzhou.Scale separation analysis reveals that the extreme-rainproducing storm was supported and maintained by the dynamic lifting of low-level converging flows from the north,south,and east of the storm.The low-level northerly flow originated from a mesoscale barrier jet on the eastern slope of the Taihang Mountain due to terrain blocking of large-scale easterly flows,which reached an overall balance with the southerly winds in association with a low-level meso-β-scale vortex located to the west of Zhengzhou.The large-scale easterly inflows that fed the deep convection via transport of thermodynamically unstable air into the storm prevented the eastward propagation of the weak,shallow cold pool.As a result,the convective storm was nearly stationary over Zhengzhou,resulting in record-breaking hourly precipitation. | Peng WEI Xin XU Ming XUE Chenyue ZHANG Yuan WANG Kun ZHAO Ang ZHOU Shushi ZHANG Kefeng ZHU | 2023 | Advances in Atmospheric Sciences2023，40，3： | 9 |
| 8 | Adefovir Dipivoxil plus Chinese Medicine in HBeAg-Positive Chronic Hepatitis B Patients:A Randomized Controlled 48-Week Trial显示文摘Objective:To evaluate the effects of a 48-week course of adefovir dipivoxil(ADV)plus Chinese medicine(CM)therapy,namely Tiaogan Jianpi Hexue(调肝健脾和血)and Tiaogan Jiedu Huashi(调肝解毒化湿)fomulae,in hepatitis B e antigen(HBeAg)-positive Chinese patients.Methods:A total of 605 HBeAg-positive Chinese CHB patients were screened and 590 eligible participants were randomly assigned to 2 groups in 1:1 ratio including experimental group(EG,received ADV plus CM)and control group(CG,received ADV plus CM-placebo)for 48 weeks.The major study outcomes were the rates of HBeAg and HBV-DNA loss on week 12,24,36,48,respectively.Secondary endpoints including liver functions(enzymes and bilirubin readings)were evaluated every 4 weeks at the beginning of week 24,36,and 48.Routine blood,urine,and stool analyses in addition to electrocardiogram and abdominal B scan were monitored as safety evaluations.Adverse events(AEs)were documented.Results:The combination therapy demonstrated superior HBeAg loss at 48 weeks,without additional AEs.The full analysis population was 560 and 280 in each group.In the EG,population achieved HBeAg loss on week 12,24,36,and 48 were 25(8.90%),34(12.14%),52(18.57%),and 83(29.64%),respectively;the equivalent numbers in the CG were 20(7.14%),41(14.64%),54(19.29%),and 50(17.86%),respectively.There was a statistically significant difference between two groups on week 48(P<0.01).No additional AEs were found in EG.Subgroup analysis suggested different outcomes among treatment patterns.Conclusion:Combination of CM and ADV therapy demonstrated superior HBeAg clearance compared with ADV monotherapy.The finding indicates that this combination therapy may provide an improved therapeutic effect and safety profile(ChiCTR-TRC-11001263). | LI Xiao-ke ZHANG Ming-xiang SHAO Feng-zhen ZHOU Da-qiao XUE Jing-dong LIU Tie-jun CHI Xiao-ling LU Bing-jiu WANG Xian-bo LI Qin LI Jun MAO De-wen YANG Hua-sheng YANG Hong-zhi ZHAO Wen-xia LI Yong ZHANG Guo-liang ZHAO Yi-ming ZOU Jian-dong LIU Meng-yang ZHANG Ke-ke YANG Xian-zhao GAN Da-nan LI Ying ZHANG Peng LI Zhi-guo LI Shuo YE Yong-an | 2020 | Chinese Journal of Integrative Medicine2020，26，5： | 9 |
| 9 | Untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease induced by high-temperature-processed feed in Sprague-Dawley rats显示文摘BACKGROUND Nonalcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases in the world.In our early clinical data and questionnaire analysis of NAFLD,it was found that the body mass index of some patients did not meet the diagnostic criteria for overweight or obesity.The consumption of high-temperature-processed foods such as fried food,hot pot and barbecue is closely related to the occurrence of nonobese NAFLD.Reducing the intake of this kind of food can reduce disease severity and improve prognosis.AIM To explore the untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease in Sprague-Dawley rats induced by high-temperatureprocessed feed.METHODS Fifty-four male Sprague-Dawley rats were divided into three groups:The control group received a standard diet;the nonfried soybeans(NDFS)group received 60%NDFS and 40%basic feed and the dry-fried soybeans(DFS)group received 60%DFS and 40%basic feed.Six rats were sacrificed at week 4,8,and 12 in each group.The food intake,body weight,Lee’s index,liver index,serological index and hepatic histopathology were assessed.Untargeted metabolomics characteristics were used to analyze the changes in liver metabolites of rats at week 12.Correlations between metabolites and pathology scores between the DFS and control groups and between the DFS and NDFS groups were analyzed.We selected some of the metabolites,both within the pathway and outside of the pathway,to explain preliminarily the difference in liver pathology in the three groups of rats.RESULTS There were no statistically significant differences in the food intake,body weight,Lee's index or serological index between the DFS group and the control group(P>0.05).At week 8 and week 12,the steatosis scores in the DFS group were significantly higher than those in the other two groups(P<0.05).At week 12,the liver index of the DFS group was the lowest(NDFS group vs DFS group,P<0.05).The fibrosis score in the DFS group was significantly higher than those in the other two groups(P<0.05).The correlation analysis of the liver pathology score and differential metabolites in the DFS and NDFS groups showed that there were 10 strongly correlated substances:Five positively correlated substances and five negatively correlated substances.The positively correlated substances included taurochenodeoxycholate-3-sulfate,acetylcarnitine,20a,22bdihydroxycholesterol,13E-tetranor-16-carboxy-LTE4 and taurocholic acid.The negatively correlated substances included choline,cholesterane-3,7,12,25-tetrol-3-glucuronide,nicotinamide adenine dinucleotide phosphate,lysoPC[16:1(9Z)]and glycerol 3-phosphate.The correlation analysis of the liver pathology score and differential metabolites in the DFS and control groups showed that there were 13 strongly correlated substances:Four positively correlated substances and 9 negatively correlated substances.The positively correlated substances included 4-hydroxy-6-eicosanone,3-phosphoglyceric acid,13-hydroxy-9-methoxy-10-oxo-11-octadecenoic acid and taurochenodeoxycholate-3-sulfate.The negatively correlated substances included lysoPC[16:1(9Z)],S-(9-hydroxy-PGA1)-glutathione,lysoPC[20:5(5Z,8Z,11Z,14Z,17Z)],SM(d18:1/14:0),nicotinamide adenine dinucleotide phosphate,5,10-methylene-THF,folinic acid,N-lactoylglycine and 6-hydroxy-5-methoxyindole glucuronide.CONCLUSION We successfully induced liver damage in rats by using a specially prepared hightemperature-processed feed and explored the untargeted metabolomics characteristics. | Li-Jun Xue Ju-Qiang Han Yuan-Chen Zhou Hong-Ye Peng Teng-Fei Yin Kai-Min Li Shu-Kun Yao | 2020 | World Journal of Gastroenterology2020，26，46： | 8 |
| 10 | Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver显示文摘Dear Editor,RNA knockdown in vivo carries significant potential for dis-ease modeling and therapies.Despite the emerging approaches of CRISPR/Cas9-mediated permanent knock out of targeted genes,strategies targeting RNA for disruption are advantageous in the treatment of acquired metabolic disorders when permanent modification of genome DNA is not appropriate,and RNA virus infection diseases when pathogenic DNA is not available(such as SARS-Cov-2 and MERS infections). | Bingbing He Wenbo Peng Jia Huang Hang Zhang Yingsi Zhou Xiali Yang Jing Liu Zhijle Li Chunlong Xu Mingxing Xue Hui Yang Pengyu Huang | 2020 | Protein & Cell2020，11，7： | 7 |
| 11 | High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/He N mice显示文摘AIM: To optimize the viral persistence rate in a hydrodynamic injection(HI) based hepatitis B virus(HBV) transfection mouse model.METHODS:(1) 5-6-wk-old male C3H/He N and C57BL/6 mice were hydrodynamically injected with 10 μg endotoxin-free p AAV/HBV1.2 plasmid DNA via the tail vein. Hepatitis B surface antigen(HBs Ag), hepatitis B e antigen(HBe Ag) and HBV DNA, both in the serum and liver, were detected at different time points post HI by ELISA, immunohistochemical staining or quantitative polymerase chain reaction(PCR);(2) male C3H/He N and C57BL/6 mice, either hydrodynamically injected mice at 10 wk post HI or na?ve mice, were all immunized subcutaneously with 5 μg HBs Ag formulated in complete Freund's adjuvant three times at a 2-wk interval. Two weeks after the final immunization, splenocytes were isolated for T cell function analysis by ELISPOT assay; and(3) five weeks post HI, C3H/He N mice were intragastrically administered 0.1 mg/kg entecavir once a day for 14 d, or were intraperitoneally injected with 1 mg/kg interferon(IFN)-α twice a week for 2 wk, or were treated with PBS as controls. The sera were collected and assayed for HBV DNA on days 0, 7 and 14 after drug treatment. RESULTS:(1) Approximately 90%(22/25) of the injected C3H/He N mice were still HBs Ag-positive at 46 wk post HI, whereas HBs Ag in C57BL/6 mice were completely cleared at 24 wk. Serum levels of HBe Ag in C3H/He N mice were higher than those in C57BL/6 mice from 4 wk to 46 wk. HBV DNA levels in the hydrodynamically injected C3H/He N mice were higher than those in the C57BL/6 mice, both in the serum(from 4 wk to 46 wk) and in the liver(detected at 8 wk and 46 wk post HI). Histology showed that hepatitis B core antigen and HBs Ag were expressed longer in the liver of C3H/He N mice than in C57BL/6;(2) HBs Ag specific T cell responses after HBs Ag vaccination in hydrodynamically injected C3H/He N and C57BL/6 mice, or naive control mice were detected by ELISPOT assay. After stimulation with HBs Ag, the frequencies of IFN-γ producing splenocytes in the hydrodynamically injected C3H/He N mice were significantly lower than those in hydrodynamically injected C57BL/6 mice, control C3H/He N and control C57BL/6 mice, which were 0, 17 ± 7, 18 ± 10, and 41 ± 10 SFCs/106 splenocytes, respectively, and the mean spot sizes showed the same pattern. Even just stimulated with PMA and ionomysin, T-cell responses elicited in the vaccinated control C3H/He N were much higher than those in hydrodynamically injected C3H/He N mice; and(3) For drug treatment experiments on the hydrodynamically injected C3H/He N mice, serum HBV DNA levels in the entecavir treatment group declined(131.2 folds, P < 0.01) on day 7 after treatment and kept going down. In the group of IFN-α treatment, serum HBV DNA levels declined to a lowest point(6.42 folds, P < 0.05) on 7 d after treatment and then rebounded.CONCLUSION: We have developed a novel HI-based HBV transfection model using C3H/He N mice, which had a higher HBV persistence rate than the classic C57BL/6 mouse model. | Xiu-Hua Peng Xiao-Nan Ren Li-Xiang Chen Bi-Sheng Shi Chun-Hua Xu Zhong Fang Xue Liu Jie-Liang Chen Xiao-Nan Zhang Yun-Wen Hu Xiao-Hui Zhou | 2015 | World Journal of Gastroenterology2015，21，12： | 7 |
| 12 | 30 GHz GeSn photodetector on SOI substrate for 2 µm wavelength application显示文摘We report the demonstration of a normal-incidence p-i-n germanium-tin(Ge_(0.951)Sn_(0.049))photodetector on silicon-on-insulator substrate for 2μm wavelength application.The DC and RF characteristics of the devices have been characterized.A dark current density under−1 V bias of approximately 125 mA/cm^(2) is achieved at room temperature,and the optical responsivity of 14 mA/W is realized for illumination wavelength of 2μm under−1 V reverse bias.In addition,a 3 dB bandwidth(f_(3dB))of around 30 GHz is achieved at−3 V,which is the highest reported value among all group III–V and group IV photodetectors working in the 2μm wavelength range.This work illustrates that a GeSn photodetector has great prospects in 2μm wavelength optical communication. | Xiuli Li Linzhi Peng Zhi Liu Zhiqi Zhou Jun Zheng Chunlai Xue Yuhua Zuo Baile Chen Buwen Cheng | 2021 | Photonics Research2021，9，4： | 6 |
| 13 | The Lyman-alpha Solar Telescope(LST) for the ASO-S mission——Ⅱ. design of LST显示文摘As one of the three payloads for the Advanced Space-based Solar Observatory(ASO-S)mission,the Lyman-alpha(Lyα)Solar Telescope(LST)is composed of three instruments:a Solar Corona Imager(SCI),a LyαSolar Disk Imager(SDI)and a full-disk White-light Solar Telescope(WST).When working in-orbit,LST will simultaneously perform high-resolution imaging observations of all regions from the solar disk to the inner corona up to 2.5 R⊙(R⊙stands for the mean solar radius)with a spatial resolution of 4.8′′and 1.2′′for coronal and disk observations,respectively,and a temporal resolution of 30–120 s and 1–120 s for coronal and disk observations,respectively.The maximum exposure time can be up to20 s due to precise pointing and image stabilization function.Among the three telescopes of LST,SCI is a dual-waveband coronagraph simultaneously and independently observing the inner corona in the HI Lyα(121.6±10 nm)line and white light(WL)(700±40 nm)wavebands by using a narrowband Lyαbeam splitter and has a field of view(FOV)from 1.1 to 2.5 R⊙.The stray-light suppression level can attain<10^-6 B⊙(B⊙is the mean brightness of the solar disk)at 1.1 R⊙and≤5×10^-8 B⊙at 2.5 R⊙.SDI and WST are solar disk imagers working in the Lyαline and 360.0 nm wavebands,respectively,which adopt an off-axis two-mirror reflective structure with an FOV up to 1.2 R⊙,covering the inner coronal edge area and relating to coronal imaging.We present the up-to-date design for the LST payload. | Bo Chen Hui Li Ke-Fei Song Quan-Feng Guo Pei-Jie Zhang Ling-Ping He Shuang Dai Xiao-Dong Wang Hai-Feng Wang Chun-Long Liu Hong-Ji Zhang Guang Zhang Yunqi Wang Shi-Jie Liu Hong-Xin Zhang Lei Liu Shi-Lei Mao Yang Liu Jia-Hao Peng Peng Wang Liang Sun Yang Liu Zhen-Wei Han Yan-Long Wang Kun Wu Guang-Xing Ding Peng Zhou Xin Zheng Ming-Yi Xia Qing-Wen Wu Jin-Jiang Xie Ya Chen Shu-Mei Song Hong Wang Bo Zhu Chang-Bo Chu Wen-Gang Yang Li Feng Yu Huang Wei-Qun Gan Ying Li Jing-Wei Li Lei Lu Jian-Chao Xue Bei-Li Ying Ming-Zhe Sun Cheng Zhu Wei-Min Bao Lei Deng Zeng-Shan Yin | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 6 |
| 14 | Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued'A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)';now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients. | Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong Yue Xiao-Chun Zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang Zhou Ya-Dong Gao Zhui Yu Feng Xu Ming-Li Tu Li-Ming Tan Min Yang Fang Chen Xiao-Ju Zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang Zhang Wei Li Wen Chen Yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong Ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang | 2020 | Military Medical Research2020，7，3： | 6 |
| 15 | Clinical features, survival and prognostic factors of primary testicular diffuse large B-cell lymphoma显示文摘Objective: To assess the clinical features, survival and prognostic factors of primary testicular diffuse large B-cell lymphoma(DLBCL).Methods: A retrospective study of 37 patients with primary testicular DLBCL was carried out from November 2003 to May 2012. Their clinical features, survival and prognostic factors were analyzed.Results: During a median follow-up period of 39.8 months(5.4-93.0 months), the median progression-free survival(PFS) was 26.2 months(95% CI: 0-65 months) and the 3-year overall survival(OS) rate was 78.4%. Within the whole cohort, the factors significantly associated with a superior PFS were limited stage(stage I/II), lactate dehydrogenase(LDH) ≤245 U/L, international prognostic index(IPI) ≤1, primary tumor diameter <7.5 cm, and patients who had complete response(CR) and received doxorubicin-contained chemotherapy(P<0.05). There was a trend toward superior outcome for patients who received combined therapy(surgery/chemotherapy/radiotherapy)(P=0.055). Patients who had CR, primary tumor diameter <7.5 cm and IPI score ≤1 were significantly associated with longer PFS at multivariate analysis.Conclusions: Primary testicular DLBCL had poorer survival. CR, primary tumor diameter and IPI were independent prognostic factors. The combined therapy of orchectomy, doxorubicin-contained chemotherapy and contralateral testicular radiotherapy(RT) seemed to improve survival. | Bo Jia Yuankai Shi Mei Dong Fengyi Feng Sheng Yang Hua Lin Liqiang Zhou Shengyu Zhou Shanshan Chen Jianliang Yang Peng Liu Yan Qin Changgong Zhang Lin Gui Lin Wang Xue Wang Xiaohui He | 2014 | Chinese Journal of Cancer Research2014，26，4： | 5 |
| 16 | Cell cycle-related kinase reprograms the liver immune microenvironment to promote cancer metastasis显示文摘The liver is an immunologically tolerant organ and a common metastatic site of multiple cancer types.Although a role for cancer cell invasion programs has been well characterized,whether and how liver-intrinsic factors drive metastatic spread is incompletely understood.Here,we show that aberrantly activated hepatocyte-intrinsic cell cycle-related kinase(CCRK)signaling in chronic liver diseases is critical for cancer metastasis by reprogramming an immunosuppressive microenvironment.Using an inducible liverspecific transgenic model,we found that CCRK overexpression dramatically increased both B16F10 melanoma and MC38 colorectal cancer(CRC)metastasis to the liver,which was highly infiltrated by polymorphonuclear-myeloid-derived suppressor cells(PMNMDSCs)and lacking natural killer T(NKT)cells.Depletion of PMN-MDSCs in CCRK transgenic mice restored NKT cell levels and their interferon gamma production and reduced liver metastasis to 2.7% and 0.7%(metastatic tumor weights)in the melanoma and CRC models,respectively.Mechanistically,CCRK activated nuclear factor-kappa B(NF-κB)signaling to increase the PMN-MDSC trafficking chemokine C-X-C motif ligand 1(CXCL1),which was positively correlated with liver-infiltrating PMN-MDSC levels in CCRK transgenic mice.Accordingly,CRC liver metastasis patients exhibited hyperaaivation of hepatic CCRK/NF-κB/CXCL1 signaling,which was associated with accumulation of PMN-MDSCs and paucity of NKT cells compared to healthy liver transplantation donors.In summary,this study demonstrates that immunosuppressive reprogramming by hepatic CCRK signaling undermines antimetastatic immunosurveillance.Our findings offer new mechanistic insights and therapeutic targets for liver metastasis intervention. | Xuezhen Zeng Jingying Zhou Zhewen Xiong Hanyong Sun Weiqin Yang Myth T.S.Mok Jing Wang Jingqing Li Man Liu Wenshu Tang Yu Feng Hector Kwong-Sang W ang Shun-Wa Tsang King-Lau Chow Philip Chun Yeung John Wong Paul Bo-San Lai Anthony Wing-Hung Chan Ka Fai To Stephen Lam Chan Qiang Xia Jing Xue Xiao Chen Jun Yu Sui Peng Joseph Jao-Yiu Sung Ming Kuang Alfred Sze-Lok Cheng | 2021 | Cellular & Molecular Immunology2021，18，4： | 5 |
| 17 | Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates显示文摘The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the disease causative agent.Vaccine is the most effective approach to eradicate a pathogen.The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations.Here we evaluated the safety,immunogenicity,and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates.Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates,and subsequently provided partial(in low dose)or full(in high dose)protection of challenge in the tested animals.In addition,passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice.These results warranted positive outcomes in future clinical trials in humans. | Yan-Feng Yao Ze-Jun Wang Ren-Di Jiang Xue Hu Hua-Jun Zhang Yi-Wu Zhou Ge Gao Ying Chen Yun Peng Mei-Qin Liu Ya-Nan Zhang Juan Min Jia Lu Xiao-Xiao Gao Jing Guo Cheng Peng Xu-Rui Shen Qian Li Kai Zhao Lian Yang Xin Wan Bo Zhang Wen-Hui Wang Jia Wu Peng Zhou Xing-Lou Yang Shuo Shen Chao Shan Zhi-Ming Yuan Zheng-Li Shi | 2021 | Virologica Sinica2021，36，5： | 4 |
| 18 | Application of regional citrate anticoagulation in patients at high risk of bleeding during intermittent hemodialysis:a prospective multicenter randomized controlled trial显示文摘Objective:Safe and effective anticoagulation is essential for hemodialysis patients who are at high risk of bleeding.The purpose of this trial is to evaluate the effectiveness and safety of two-stage regional citrate anticoagulation(RCA)combined with sequential anticoagulation and standard calcium-containing dialysate in intermittent hemodialysis(IHD)treatment.Methods:Patients at high risk of bleeding who underwent IHD from September 2019 to May 2021 were prospectively enrolled in 13 blood purification centers of nephrology departments,and were randomly divided into RCA group and saline flushing group.In the RCA group,0.04 g/mL sodium citrate was infused from the start of the dialysis line during blood draining and at the venous expansion chamber.The sodium citrate was stopped after 3 h of dialysis,which was changed to sequential dialysis without anticoagulant.The hazard ratios for coagulation were according to baseline.Results:A total of 159 patients and 208 sessions were enrolled,including RCA group(80 patients,110 sessions)and saline flushing group(79 patients,98 sessions).The incidence of severe coagulation events of extracorporeal circulation in the RCA group was significantly lower than that in the saline flushing group(3.64%vs.20.41%,P<0.001).The survival time of the filter pipeline in the RCA group was significantly longer than that in the saline flushing group((238.34±9.33)min vs.(221.73±34.10)min,P<0.001).The urea clearance index(Kt/V)in the RCA group was similar to that in the saline flushing group with no statistically significant difference(1.12±0.34 vs.1.08±0.34,P=0.41).Conclusions:Compared with saline flushing,the two-stage RCA combined with a sequential anticoagulation strategy significantly reduced extracorporeal circulation clotting events and prolonged the dialysis time without serious adverse events. | Xiaoyan TANG Dezheng CHEN Ling ZHANG Ping FU Yanxia CHEN Zhou XIAO Xiangcheng XIAO Weisheng PENG Li CHENG Yanmin ZHANG Hongbo LI Kehui LI Bizhen GOU Xin WU Qian YU Lijun JIAN Zaizhi ZHU Yu WEN Cheng LIU Hen XUE Hongyu ZHANG Xin HE Bin YAN Liping ZHONG Bin HUANG Mingying MAO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，11： | 4 |
| 19 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 20 | Dihydro-stilbene gigantol relieves CCl4-induced hepatic oxidative stress and inflammation in mice via inhibiting C5b-9 formation in the liver显示文摘In general,anti-inflammatory treatment is considered for multiple liver diseases despite the etiology.But current drugs for alleviating liver inflammation have defects,making it necessary to develop more potent and safer drugs for liver injury.in this study,we screened a series of(dihydro-)stillene or(dihydro-)phenanthrene derivatives extracted from Pholidota chinensis for theirpotential biological activities.Among 31 compounds,the dihydro-stlbene gigantol exerted most potent protective effects onhuman hepatocytes against lithocholic acid toxicity,and exhibited solid antioxidative and anti-inflammatory effect in vitro.In micewith CCl-induced acute liver injury,pre-administration of gigantol(10,20,40 mg kg^-1·d^-1,po,for 7 days)dose-dependentydecreased serum transaminase levels and improved pathological changes in liver tissues.The elevated lipid peroxidation andinflammatory responses in the livers were also signifhcantly alleviated by gigantol.The pharmacokinetic studies showed thatgigantol was highly concentrated in the mouse lvers,which consisted with its efficacy in preventing liver injury.Using a label-freequantitative proteomic analysis we revealed that gigantol mainly regulated the immune system process in liver tissues of CCl4-treated mice,and the complement and coagulation cascades was the predominant pathway gigantol markedly inhibited theexpression of complement component C9,which was a key component for the formation of terminal complerment complex(TCC)C5b-9.These results were validated by immunohistochemistry(IHC) or real time-PCR.Confocal microscopy analysis showed thatgigantol significantly inhibited the vascular deposition of TCC in the liver.In conclusion,we demonstrate for the first time that oral administration of gigantol potently relieves liver oxidative stress and inflammation,possibly via a novel mechanism of inhibiting the C5b-9 formation in the liver. | Ya-ru Xue Sheng Yao Qian Liu Zhao-liang Peng Qiang-qiang Deng—Bo Liu Zheng-hua Ma Le Wangf Hu Zhou Yang Ye Guo-yu Pan | 2020 | Acta Pharmacologica Sinica2020，41，11： | 3 |
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