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| 1 | Enhanced Poly(ethylene terephthalate)Hydrolase Activity by Protein Engineering显示文摘Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the issue of polyester plastic pollution.Therefore,we explored the interaction between PETase and the substrate(a dimer of the PET monomer ethylene terephthalate,2PET),using a model of PETase and its substrate.In this study,we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering.These PETase mutants were designed and tested.The enzymatic activities of the R61A,L88F,and I179F mutants,which were obtained with a rapid cell-free screening system,exhibited 1.4 fold,2.1 fold,and 2.5 fold increases,respectively,in comparison with wild-type PETase.The I179F mutant showed the highest activity,with the degradation rate of a PET film reaching 22.5 mg perμmol·L^-1 PETase per day.Thus,this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites,and has further illustrated the potential of biodegradable plastics. | Yuan Ma Mingdong Yao Bingzhi Li Mingzhu Ding Bo He Si Chen Xiao Zhou Yingjin Yuan | 2018 | Engineering2018，4，6： | 10 |
| 2 | Shuganjieyu capsule increases neurotrophic factor expression in a rat model of depression显示文摘Shuganjieyu capsule has been approved for clinical treatment by the State Food and Drug Administration of China since 2008. In the clinic, Shuganjieyu capsule is often used to treat mild to moderate depression. In the rat model of depression established in this study, Shuganjieyu capsule was administered intragastrically daily before stress. Behavioral results confirmed that depressive symptoms lessened after treatment with high-dose(150 mg/kg) Shuganjieyu capsule. Immunohistochemistry results showed that high-dose Shuganjieyu capsule significantly increased phosphorylation levels of phosphorylation cyclic adenosine monophosphate response element binding protein and brain-derived neurotrophic factor expression in the medial prefrontal cortex and hippocampal CA3 area. Overall, our results suggest that in rats, Shuganjieyu capsule effectively reverses depressive-like behaviors by increasing expression levels of neurotrophic factors in the brain. | Jinhua Fu Yingjin Zhang Renrong Wu Yingjun Zheng Xianghui Zhang Mei Yang Jingping Zhao Yong Liu | 2014 | Neural Regeneration Research2014，9，5： | 10 |
| 3 | Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations显示文摘Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzyme is required. By recruiting two enzymes—HpaB and HpaC—from several bacteria, we constructed functional 4- hydroxyphenylacetate 3-hydroxylase (4HPA3H) in Saccharomyces cerevisiae to take on a role similar to that of the plant-derived cytochrome P450 enzyme and produce caffeic acid. Along with a common tyrosine ammonia lyase (TAL), the different combinations of HpaB and HpaC presented varied capabilities in producing the target product, caffeic acid, from the substrate, L-tyrosine. The highest production of caffeic acid was obtained with the enzyme combination of HpaB from Pseudomonas aeruginosa and HpaC from Salmonella enterica, which yielded up to (289.4 ± 4.6) mg-L1 in shake-flask cultivation. The compatibility of heterologous enzymes within a yeast chassis was effectively improved, as the caffeic acid production was increased by 40 times from the initial yield. Six key amino acid residues around the flavin adenine dinucleotide (FAD) binding domain in HpaB from Pseudomonas aeruginosa were differentiate from those other HpaBs, and might play critical roles in affecting enzyme activity. We have thus established an effective approach to construct a highly efficient yeast system to synthesize non-native hydroxylated phenylpropanoids. | Lanqing Liu Hong Liu Wei Zhang Mingdong Yao Bingzhi Li Duo Liu Yingjin Yuan | 2019 | Engineering2019，5，2： | 8 |
| 4 | Engineering of β-carotene hydroxylase and ketolase for astaxanthin overproduction in Saccharomyces cerevisiae显示文摘到 astaxanthin 的叶红素的变换是一个复杂小径网络, hydroxylation 的二步和 ketolation 的二步被叶红素 hydroxylase (CrtZ ) 和叶红素 ketolase (CrtW ) 分别地在催化。这里, astaxanthin 生合成小径被介绍 heterologous CrtZ 和 CrtW 进生产紧张的存在高叶红素在 Saccharomyces cerevisiae 构造。基因 crtZ 和 crtW 是在组成的倡导者的控制下面优化并且表示的 codon。通过从多样的种类的 CrtZ 和 CrtW 的组合表示,穿黑暗的红衣服的九紧张视觉上从三十联合被选择。在所有选择紧张,从 Alcaligenes sp 从 Brevundimonas vesicularis DC263 和 CrtZ 与 CrtW 拉紧 SyBE_Sc118060。紧张 PC-1 完成了 3.1 mg/g DCW 的最高的 astaxanthin 收益。蛋白质种系发生的分析看那 CrtW 的进化距离越短, astaxanthin titer 是越多 higher。进一步,当在紧张 SyBE_Sc118060 的 crtZ 的倡导者从 FBA1p 被代替到 TEF1p 时, astaxanthin 收益被 30.4% 增加(从 3.4 ~ 4.5 mg/g DCW ) 。同时, crtZ 抄写水平上的 33.5 褶层增加和到 crtW 的 crtZ 的 transcriptional 比率上的 39.1 褶层改进与 4% 在媒介在早指数的阶段被观察(w/v ) 葡萄糖。不同尽管到 crtW 的 crtZ 的比率被增加在中间 -- ,在有 2% 的媒介(w/v ) 的迟了指数的阶段葡萄糖, crtZ 和 crtW 的抄写水平实际上被减少在期间自始至终路线,因而不在 astaxanthin 上导致重要改进生产。通过用碳来源限制策略的高房间密度喂批发酵,最后,在一个 5-L 生物反应器的 astaxanthin 的生产到达了到 81.0 mg/L,它是在酵母报导的最高的 astaxanthin titer。这研究提供一本参考书极大地由在微生物采用关键酶提高需要的混合物累积。 | Ruizhao Wang Xiaoli Gu Mingdong Yao Caihui Pan Hong Liu Wenhai Xiao Ying Wang Yingjin Yuan | 2017 | Frontiers of Chemical Science and Engineering2017，11，1： | 5 |
| 5 | Fracture Characteristics and Heat Accumulation of Jixianian Carbonate Reservoirs in the Rongcheng Geothermal Field, Xiong'an New Area显示文摘Geothermal energy plays an important role in urban construction of the Xiong’an New Area. Geothermal reservoir fracture distribution of the Mesoproterozoic Jixianian Wumishan Formation(Fm.) carbonate reservoir in the Rongcheng geothermal field are evaluated based on FMI log from Wells D19 and D21. The results show carbonate reservoir fracture density of Well D19 is 15.2/100 m, greater than that of Well D21 with a value of 9.2/100 m. Reservoir porosity and permeability of Well D19 are better than that of Well D21, and the water saturation is bimodally distributed. The movable fluid volume ratio(BVM) of Well D19 is 2% to 8% with some zones exceeding 20%, while the value of Well D21 is less than 4%. Therefore, reservoir fractures in Well D19 are more conducive to fluid flow. Reservoir fractures have a similar occurrence to normal faults, indicating that the tensile stress field controlled the formation of such fractures. Developed reservoir fractures provide a good channel for groundwater convection. The circulation of regional groundwater and the heat exchange between water and rock and the multiple heat accumulation patterns form a stable and high potential heat reservoir in the Rongcheng geothermal field. | WANG Yingjin MA Feng XIE Heping WANG Guiling WANG Zhihe | 2021 | Acta Geologica Sinica(English Edition)2021，95，6： | 5 |
| 6 | Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain显示文摘Peripheral nerve injury could lead to chronic neuropathic pain.Understanding transcriptional changes induced by nerve injury could provide fundamental insights into the complex pathogenesis of neuropathic pain.Gene expression profiles of dorsal root ganglia(DRG)in neuropathic pain condition have been studied.However,little is known about transcriptomic changes in individual DRG neurons after peripheral nerve injury. | Kaikai Wang Sashuang Wang Yan Chen Dan Wu Xinyu Hu Yingjin Lu Liping Wang Lan Bao Changlin Li Xu Zhang | 2021 | Cell Research2021，31，8： | 5 |
| 7 | Medium Optimization for Antifungal Active Substance Production from Streptomyces Lydicus Using Response Surface Methodology显示文摘Response surface methodology was used to optimize the medium for antifungal active substance production from Streptomyces lydicus E12 in flask cultivation.Initially,the component factors,which influence antifungal substance production,were studied by varying one factor at a time.Starch,soybean cake powder,K_2HPO_4·3H_2O and MgSO_4·7H_2O were found to have a significant effect on the production of antifungal substances by the traditional design.Then,a Box-Behnken design was applied for further optimization.A quadratic model was found to fit antifungal active substance production.The analysis revealed that the optimum values of the tested variable were starch 84.96 g/L,soybean cake powder 4.13 g/L,glucose 5 g/L,MgSO_4·7H_2O 1.23 g/L,K_2HPO_4·3H_2O2.14 g/L and NaCl 0.5 g/L.The test result of 67.44%antifungal inhibition agreed with the prediction and increased by 14.28%in comparison with the basal medium. | Yan Zhao Yingquan Liang Lei Liu Jingsheng Cheng Yingjin Yuan | 2017 | Transactions of Tianjin University2017，23，1： | 5 |
| 8 | Optical properties of human normal small intestine tissue determined by Kubelka-Munk method in vitro显示文摘AIM: To study the optical properties of human normal small intestine tissue at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm,532 nm, 808 nm wavelengths of laser irradiation.METHODS: A double-integrating-sphere system, the basic principle of measuring technology of light radiation, and an optical model of biological tissues were used in the study.RESULTS: The results of measurement showed that there were no significant differences in the absorption coefficients of human normal small intestine tissue at 476.5 nm, 488 nm,496.5 nm laser in the Kubelka-Munk two-flux model (P>0.05).The absorption coefficients of the tissue at 514.5 nm, 532 nm,808 nm laser irradiation were obviously increased with the decrease of these wavelengths. The scattering coefficients of the tissue at 476.5 nm, 488 nm, 496.5 nm laser irradiation were increased with the decrease of these wavelengths.The scattering coefficients at 496.5 nm, 514.5 nm, 532 nm laser irradiation were obviously increased with the increase of these wavelengths. The scattering coefficient of the tissue at 532 nm laser irradiation was bigger than that at 808 nm.There were no significant differences in the total attenuation coefficient of the tissue at 476.5 nm and 488 nm laser irradiation (P>0.05). The total attenuation coefficient of the tissue at 488 nm, 496.5 nm, 514.5 nm, 532 nm, 808 nm laser irradiation was obviously increased with the decrease of these wavelengths, and their effective attenuation coefficient revealed the same trend. There were no significant differences among the forward scattered photon fluxe,backward scattered photon fiuxe, and total scattered photon fiuxe of the tissue at 476.5 nm, 488 nm, 496.5 nm laser irradiation. They were all obviously increased with attenuation of tissue thickness. The attenuations of forward and backward scattered photon fluxes, and the total scattered photon fiuxe of the tissue at 514.5 nm laser irradiation were slower than those at 476.5 nm, 488 nm, 496.5 nm laser irradiation respectively. The attenuations of forward and backward scattered photon fluxes, and total scattered photon fluxes at 532 nm laser irradiation were obviously slower than those at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm laser irradiation. The attenuations of forward and backward scattered photon fluxes, and total scattered photon fiuxe at 808 nm laser irradiation were all obviously slower than those at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm, 532 nm laser irradiation respectively.CONCLUSION: There are significant differences in optical parameters of human normal small intestine tissue in the Kubelka-Munk two-flux model at six different wavelengths of laser radiation. The results would provide a new method of information analysis for clinical diagnosis. | Hua-JiangWei DaXing Guo-YongWu YingJin Huai-MinGu | 2003 | World Journal of Gastroenterology2003，9，9： | 4 |
| 9 | Loss of heterozygosity by SCRaMbLEing显示文摘Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE,different levels of loss of heterozygosity(LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome Ⅹ, whole chromosome LOH of synthetic chromosome Ⅹ, and duplication of chromosome Ⅷ(trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function. | Yunxiang Li Yi Wu Lu Ma Zhou Guo Wenhai Xiao Yingjin Yuan | 2019 | Science China(Life Sciences)2019，62，3： | 4 |
| 10 | Effect of tribulus terrestris saponins on behavior and neuroendocrine in chronic mild stress depression rats显示文摘OBJECTIVE:To observe the effect of tribulus terrestris saponins(TTS) on behavior and neuroendocrine of chronic mild stress(CMS) depression rats.METHODS:ThirtymaleSprague-Dawleyratswererandomly allocated to six groups:vehicle group,CMS group,CMS + fluoxetine group and CMS + TTS of low-dosage(0.375g/kg),medium-dosage(0.75g/kg) and high-dosage(2.25 g/kg) groups.All rats except the vehicle group singly housed and exposed an unpredicted sequence of mild stressors.The behavior of rats was detected by open-field test(OFT) and sucrose preference test(SPT).The concentration of corticotropin-releasing factor(CRF) and adrenocorticotropic hormone(ACTH) in serum of the rats were detected by radioimmunoassay.The concentration of cortisol(CORT) in serum was detected by enzyme immunoassay.RESULTS:CMS procedure not only significantly decreased the scores of crossing,rears and grooming in OFT and the sucrose preference in SPT(all P< 0.01),but also markedly increased serum CRH and CORT levels(both P<0.05).Treatment with TTS(0.75 and 2.25 g/kg) could significantly prevent all of these abnormalities induced by CMS(P<0.05,P< 0.01).CONCLUSION:CMS can affect rat behavior and neuroendocrine and cause depression.TTS has the antagonism on CMS and produce antidepressive effects. | Zhe Wang Dongdong Zhang Shan Hui Yingjin Zhang Suiyu Hu | 2013 | Journal of Traditional Chinese Medicine2013，33，2： | 4 |
| 11 | Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica显示文摘Genomic variants libraries are conducive to obtain dominant strains with desirable phenotypic traits.The non-homologous end joining(NHEJ),which enables foreign DNA fragments to be randomly integrated into different chromosomal sites,shows prominent capability in genomic libraries construction.In this study,we established an efficient NHEJ-mediated genomic library technology in Yarrowia lipolytica through regulation of NHEJ repair process,employment of defective Ura marker and optimization of iterative transformations,which enhanced genes integration efficiency by 4.67,22.74 and 1.87 times,respectively.We further applied this technology to create high lycopene producing strains by multi-integration of heterologous genes of CrtE,CrtB and CrtI,with 23.8 times higher production than rDNA integration through homologous recombination(HR).The NHEJ-mediated genomic library technology also achieved random and scattered integration of loxP and vox sites,with the copy number up to 65 and 53,respectively,creating potential for further application of recombinase mediated genome rearrangement in Y.lipolytica.This work provides a high-efficient NHEJ-mediated genomic library technology,which enables random and scattered genomic integration of multiple heterologous fragments and rapid generation of diverse strains with superior phenotypes within 96 h.This novel technology also lays an excellent foundation for the development of other genetic technologies in Y.lipolytica. | Qiuyan Bai Shuai Cheng Jinlai Zhang Mengxu Li Yingxiu Cao Yingjin Yuan | 2021 | Science China(Life Sciences)2021，64，12： | 4 |
| 12 | Influence of cosmic radiation on lymphocyte micronucleus,serum lipid peroxide and antioxidation capacity in aircrew members显示文摘This study looks into the influence of cosmic radiation at high altitudes on human bodies. Results reveal that the cytokinesis-block micronuclei (CBMN) and conventional cultured micronuclei in peripheral blood lymphocytes, serum levels of lipid peroxide, superoxide dismutase, and the total antioxidation capacity by chemical colorimetry all increased significantly in aircrew members. There exists a linear relationship between the CBMN and the average annual effective doses of radiation received or the average annual flying hours. With both of them, a trend shows that the serum lipid peroxide levels increase as well. Either the lipid peroxide or CBMN can sensitively reflect the recent changes in flight load. These findings indicate that cosmic radiation impairs the stability of chromosomes and genome, and induces lipid oxidative damage in aircrews; Lymphocyte CBMN and serum lipid peroxide can be used as monitoring indicators in the cosmic radiation protection for aircrew members. | CHEN Weiru FENG Yingjin LIU Jiangkuang ZHANG Haili GUO Ying ZHANG Yan DUAN Shiying PENG Xintao SUN Tianping JIA Baoshan ZHAO Junxian LI Hua | 2002 | Chinese Science Bulletin2002，47，8： | 3 |
| 13 | Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae显示文摘当先锋 l 酷氨酸被一个复杂 biosynthetic 系统紧地调整,在 Saccharomyces cerevisiae 的 heterologous resveratrol 生产的新陈代谢的工程面对挑战。我们主要基因在在上游的小径指向在下游的 resveratrol 生产上调查他们的影响的 overexpressed。单个基因的 overexpression 和 DNA 指导集会的 multigene overexpression 影响 resveratrol 以及它的先锋 p-coumaric 酸的生产。最后,选择基因目标的合作导致 66.14 的最佳的 resveratrol 生产 ? 铬 ? 铬 ? | Duo Liu Bingzhi Li Hong Liu Xuejiao Guo Yingjin Yuan | 2017 | Frontiers of Chemical Science and Engineering2017，11，1： | 3 |
| 14 | Design, analysis and application of synthetic microbial consortia显示文摘The rapid development of synthetic biology has conferred almost perfect modification on single cells,and provided methodological support for synthesizing microbial consortia,which have a much wider application potential than synthetic single cells.Co-cultivating multiple cell populations with rational strategies based on interacting relationships within natural microbial consortia provides theoretical as well as experimental support for the successful obtaining of synthetic microbial consortia,promoting it into extensive research on both industrial applications in plenty of areas and also better understanding of natural microbial consortia.According to their composition complexity,synthetic microbial consortia are summarized in three aspects in this reviewand are discussed in principles of design and construction,insights and methods for analysis,and applications in energy,healthcare,etc. | Xiaoqiang Jia Chang Liu Hao Song Mingzhu Ding Jin Du Qian Ma Yingjin Yuan | 2016 | Synthetic and Systems Biotechnology2016，1，2： | 3 |
| 15 | An artificial chromosome for data storage显示文摘DNA digital storage provides an alternative for information storage with high density and long-term stability.Here,we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip.The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval.The entire 254 kb sequence was 95.27%occupied by encoded data.The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences.The stability was demonstrated by transmitting the data-carrying chromosome to the 100 th generation.This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals,similar to a compact disc,with potential in economically massive data distribution. | Weigang Chen Mingzhe Han Jianting Zhou Qi Ge Panpan Wang Xinchen Zhang Siyu Zhu Lifu Song Yingjin Yuan | 2021 | National Science Review2021，8，5： | 3 |
| 16 | Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys显示文摘An effective HIV-1 vaccine will be the ultimate solution for the prevention of HIV/AIDS, though HAART plays important roles in treating the disease. In this study, a large-scale recombinant DNA plasmid containing a designed HIV-1 multi-epitope- p24 chimeric gene was prepared and purified. Rhesus monkeys were then inoculated muscularly with the plasmid for four times in week 0, 4, 8 and 18. Whole blood was collected two weeks after the third and fourth inoculation, followed by serum and pe- ripheral blood mononuclear cell (PBMC) separation. The CTL activity and proliferation of PBMCs stimu- lated by macaque MHC-I-restricted HIV-1 CTL epi- tope peptide were analyzed by MTT and LDH release assay, respectively. Th1 cytokines in supernatant of cultured PBMC stimulated by HIV-1 CTL epitope peptide and anti-HIV-1 antibody in serum were as- sayed by ELISA. The results showed that increased CTL target-killing activity, higher secretion of Th1 cytokines (IFN-γ and IL-2) and promoted proliferative reaction of monkey PBMCs stimulated by HIV-1 CTL epitope peptide were detected in the immunization group inoculated by the recombinant DNA vaccine for three times, which were further enhanced by the fourth inoculation. At the same time, HIV-1 specific antibody in serum of immunized monkeys was higher than that in controls. We concluded that the designedHIV-1 DNA vaccine may induce HIV-1 specific cellular and humoral immunity on monkeys. | ZHANG Lishu JIN Ningyi SONG Yingjin SUN Yansong WANG Hong ZHAN Dawei MA Hewen SHANG Yupu JIN Hongtao HONG Baoqing LI Chang | 2006 | Chinese Science Bulletin2006，51，13： | 3 |
| 17 | Determination of optical properties of normal and adenomatous human colon tissues in vitro using integrating sphere techniques显示文摘AIM: The purpose of the present study is to compare the optical properties of normal human colon mucosa/submucosa and muscle layer/chorion, and adenomatous human colon mucosa/submucosa and muscle layer/chorion in vitro at 476.5, 488, 496.5, 514.5 and 532 nm. We believe these differences in optical properties should help differential diagnosis of human colon tissues by using optical methods.METHODS: In vitro optical properties were investigated for four kinds of tissues: normal human colon mucosa/submucosa and muscle layer/chorion, and adenomatous human colon mucosa/submucosa and muscle layer/chorion. Tissue samples were taken from 13 human colons (13 adenomatous, 13 normal). From the normal human colons a total of 26 tissue samples, with a mean thickness of 0.40 mm, were used (13 from mucosa/submucosa and 13 from muscle layer/chorion), and from the adenomatous human bladders a total of 26 tissue samples, with a mean thickness of 0.40 mm, were used (13 from mucosa/submucosa and 13 from muscle layer/chorion). The measurements were performed using a double-integratingsphere setup and the optical properties were assessed from these measurements using the adding-doubling method that was considered reliable.RESULTS: The results of measurement showed that there were significant differences in the absorption coefficients and scattering coefficients between normal and adenomatous human colon mucosa/submucosa at the same wavelength,and there were also significant differences in the two optical parameters between both colon muscle layer/chorion at the same wavelength. And there were large differences in the anisotropy factors between both colon mucosa/submucosa at the same wavelength, there were also large differences in the anisotropy factors between both colon muscle layer/chorion at the same wavelength.There were large differences in the value ranges of the absorption coefficients, scattering coefficients and anisotropy factors between both colon mucosa/submucosa,and there were also large differences in these value ranges between both colon muscle layer/chorion. There are the same orders of magnitude in the absorption coefficients for four kinds of colon tissues. The scattering coefficients of these tissues exceed the absorption coefficients by at least two orders of magnitude.CONCLUSION: There were large differences in the three optical parameters between normal and adenomatous human colon mucosa/submucosa at the same laser wavelength, and there were also large differences in these parameters between both colon muscle layer/chorion at the same laser wavelength. Large differences in optical parameters indicate that there were large differences in compositions and structures between both colon mucosa/submucosa, and between both colon muscle layer/chorion.Optical parameters for four kinds of colon tissues are wavelength dependent, and these differences would be useful and helpful in clinical applications of laser and tumors photodynamic therapy (PDT). | Hua-JiangWei DaXing Jian-JunLu Huai-MinGu Guo-YongWu YingJin | 2005 | World Journal of Gastroenterology2005，11，16： | 2 |
| 18 | Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE显示文摘Varied environmental stress can affect cell growth and activity of the cellular catalyst.Traditional path of adaptive evolution generally takes a long time to achieve a tolerance phenotype,meanwhile,it is a challenge to dissect the underlying genetic mechanism.Here,using SCRaMbLE,a genome scale tool to generate random structural variations,a total of 222 evolved yeast strains with enhanced environmental tolerances were obtained in haploid or diploid yeasts containing six synthetic chromosomes.Whole genome sequencing of the evolved strains revealed that these strains generated different structural variants.Notably,by phenotypic-genotypic analysis of the SCRaMbLEd strains,we find that a deletion of gene YFR009W(GCN20)can improve salt tolerance of Saccharomyces cerevisiae,and a deletion of gene YER056C can improve 5-flucytosine tolerance of Saccharomyces cerevisiae.This study shows applications of SCRaMbLE to accelerate phenotypic evolution for varied environmental stress and to explore relationships between structural variations and evolved phenotypes. | Jianping Kang Jieyi Li Zhou Guo Sijie Zhou Shuxin Su Wenhai Xiao Yi Wu Yingjin Yuan | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 2 |
| 19 | Collaborations of China with the world in Synbio显示文摘 | Yingjin Yuan Jinchuan Wu Xiao Wang | 2017 | Frontiers of Chemical Science and Engineering2017，11，1： | 2 |
| 20 | Directed yeast genome evolution by controlled introduction of trans-chromosomic structural variations显示文摘Naturally occurring structural variations(SVs)are a considerable source of genomic variation that can reshape the 3D architecture of chromosomes.Controllable methods aimed at introducing the complex SVs and their related molecular mechanisms have remained farfetched.In this study,an SV-prone yeast strain was developed using Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution(SCRaMbLE)technology with two synthetic chromosomes,namely synV and synX.The biosynthesis of astaxanthin is used as a readout and a proof of concept for the application of SVs in industries.Our findings showed that complex SVs,including a pericentric inversion and a trans-chromosome translocation between synV and synX,resulted in two neo-chromosomes and a 2.7-fold yield of astaxanthin.Also,genetic targets were mapped,which resulted in a higher astaxanthin yield,thus demonstrating the SVs’ability to reorganize genetic information along the chromosomes.The rational design of trans-chromosome translocation and pericentric inversion enabled precise induction of these phenomena.Collectively,this study provides an effective tool to not only accelerate the directed genome evolution but also to reveal the mechanistic insight of complex SVs for altering phenotypes. | Bin Jia Jin Jin Mingzhe Han Bingzhi Li Yingjin Yuan | 2022 | Science China(Life Sciences)2022，65，9： | 1 |
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