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| 1 | Parameterizing soil organic carbon's impacts on soil porosity and thermal parameters for Eastern Tibet grasslands显示文摘This study investigates the stratification of soil thermal properties induced by soil organic carbon (SOC) and its impacts on the parameterization of the thermal properties. Soil parameters were measured for alpine grassland stations and North China flux stations, with a total of 34 stations and 77 soil profiles. Measured data indicate that the topsoils of alpine grasslands contain high SOC contents than underlying soil layers, which leads to higher soil porosity values and lower thermal conductivity and bulk density values in the topsoils. However, this stratification is not evident at the lowland stations due to low SOC contents. Evaluations against measured data show that three thermal conductivity schemes used in land surface models severely overestimate the values for soils with high SOC content (i.e. topsoils of alpine grassland), but they are better for soils with low SOC content. A new parameterization is then developed to take the impacts of SOC into account. The new one can well estimate the soil thermal conductivity values in both low and high SOC content cases, and therefore, it is a potential candidate of thermal conductivity scheme to be used in land surface models. | CHEN YingYing YANG Kun TANG WenJun QIN Jun ZHAO Long | 2012 | Science China Earth Sciences2012，55，6： | 31 |
| 2 | Exosome-guided bone targeted delivery of Antagomir-188 as an anabolic therapy for bone loss显示文摘The differentiation shift from osteogenesis to adipogenesis of bone marrow mesenchymal stem cells(BMSCs)characterizes many pathological bone loss conditions.Stromal cell-derived factor-1(SDF1)is highly enriched in the bone marrow for C-X-C motif chemokine receptor 4(CXCR4)-positive hematopoietic stem cell(HSC)homing and tumor bone metastasis.In this study,we displayed CXCR4 on the surface of exosomes derived from genetically engineered NIH-3T3 cells.CXCR4+exosomes selectively accumulated in the bone marrow.Then,we fused CXCR4+exosomes with liposomes carrying antagomir-188 to produce hybrid nanoparticles(NPs).The hybrid NPs specifically gathered in the bone marrow and released antagomir-188,which promoted osteogenesis and inhibited adipogenesis of BMSCs and thereby reversed age-related trabecular bone loss and decreased cortical bone porosity in mice.Taken together,this study presents a novel way to obtain bone-targeted exosomes via surface display of CXCR4 and a promising anabolic therapeutic approach for age-related bone loss. | Yan Hu Xiaoqun Li Qin Zhang Zhengrong Gu Ying Luo Jiawei Guo Xiuhui Wang Yingying Jing Xiao Chen Jiacan Su | 2021 | Bioactive Materials2021，6，9： | 12 |
| 3 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of the management of high-risk population显示文摘Aim Cerebrovascular disease is the leading cause of death and disability in China,causing a huge burden among patients and their families.Hence,stroke prevention is critical,especially in the high-risk population.Here,we present the evidence-based guideline suitable for the Chinese population.Methods Literature search of PubMed and Cochrane library(from January 1964 to June 2019)was done.After thorough discussion among the writing group members,recommendations were listed and summarised.This guideline was reviewed and discussed by the fellow writing committees of the Chinese Stroke Association’s Stroke.Results This evidence-based guideline was written in three parts:controlling the risk factors of stroke,utilisation of antiplatelet agents and assessing the risks of first-ever stroke.All recommendations were listed along with the recommending classes and levels of evidence.Conclusions This guideline provides recommendations for primary prevention of cerebrovascular disease among high-risk population in China.Controlling related risk factors,appropriately using antiplatelet agents,assessing the risk of developing first-ever stroke should help reduce the rate of cerebrovascular disease in China. | Yilong Wang Shangrong Han Haiqiang Qin Huaguang Zheng Bin Jiang Yong Cao Yuan Gao Ling Guan Qian Jia Yong Jiang Yuming Jiao Shuya Li Yapeng Li Zixiao Li Wei Liu Xiaojuan Ru Dongling Sun Haixin Sun Penglian Wang Tingting Wang Lixia Zong Lei Guo Xuewei Xie Yuming Xu Yuyuan Xu Xiaomeng Yang Yingying Yang Mengyuan Zhou Wenzhi Wang Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 11 |
| 4 | Myeloid-specific targeting of Notch ameliorates murine renal fibrosis via reduced infiltration and activation of bone marrowderived macrophage显示文摘Macrophages play critical roles in renal fibrosis.However,macrophages exhibit ontogenic and functional heterogeneities,and which population of macrophages contributes to renal fibrosis and the underlying mechanisms remain unclear.In this study,we genetically targeted Notch signaling by disrupting the transcription factor recombination signal binding protein-JK(RBP-J),to reveal its role in regulation of macrophages during the unilateral ureteral obstruction(UUO)-induced murine renal fibrosis.Myeloid-specific disruption of RBP-J attenuated renal fibrosis with reduced extracellular matrix deposition and myofibroblast activation,as well as attenuated epithelial-mesenchymal transition,likely owing to the reduced expression of TGF-β.Mean while,RBP-J deletion significantly hampered macrophage infiltration and activation in fibrotic kidney,although their proliferation appeared unaltered.By using macrophage clearance experiment,we found that kidney resident macrophages made negligible contribution,but bone marrow(BM)-derived macrophages played a major role in renal fibrogenesis.Further mechanistic analyses showed that Notch blockade reduced monocyte emigration from BM by down-regulating CCR2 expression.Finally,we found that myeloid-specific Notch activation aggravated renal fibrosis,which was mediated by CCR2^+macrophages infiltration.In summary,our data have unveiled that myeloid-specific targeting of Notch could ameliorate renal fibrosis by regulating BM-derived macrophages recruitment and activation,providing a novel strategy for intervention of this disease. | Yali Jiang Yuanyuan Wang Pengfei Ma Dongjie An Junlong Zhao Shiqian Liang Yuchen Ye Yingying Lu Peng Zhang Xiaowei Liu Hua Han Hongyan Qin | 2019 | Protein & Cell2019，10，3： | 10 |
| 5 | Development of a daily soil moisture product for the period of2002–2011 in Chinese mainland显示文摘Soil moisture is an essential climate variable(ECV) concerned widely. Due to its high spatial variability, it is costly to measure soil moisture at tens of kilometers scale. In this study, a ten-year(2002–2011) daily soil moisture dataset at 0.25° spatial resolution for Chinese mainland was produced through assimilating the Advanced Microwave Scanning Radiometer for Earth Observing System(AMSR-E) brightness temperature(TB) data into a land surface model(LSM). The obtained soil moisture data was evaluated against soil moisture-measuring networks deployed in two wet areas and one dry area of the Tibetan Plateau.The results show that for the wet areas the accuracy of the soil moisture product obtained from the assimilation is considerably higher than that of both AMSR-E official soil moisture products and land surface simulation results, and for the dry area their accuracy is comparable to each other. The spatial pattern of the soil moisture from the new product is consistent with that of soil porosity from an independent survey-based dataset, further confirming the credibility of the new product. According to this product, the transition regions in China show stronger seasonal variation of soil moisture than dry and wet regions, and drier regions have stronger inter-annual variability of soil moisture than wetter regions, particularly during transitional seasons(spring and autumn). The soil moisture product is accessible at the National Tibetan Plateau Data Center. | Kun YANG Yingying CHEN Jie HE Long ZHAO Hui LU Jun QIN Donghai ZHENG Xin LI | 2020 | Science China Earth Sciences2020，63，8： | 10 |
| 6 | Self-adjusted elastic action and its CBM pool-forming effect of the high rank coal reservoir显示文摘The coal reservoir is a kind of geologic body with considerable elasticity, and its response to stress is more sensitive than the routine ones. In the pool-forming process of the coalbed methane, (CBM) the action of many external geodynamic factors in the coal reservoir is represented by the ability of CBM’s diffusion and migration in the coal reservoir. Therefore, the occurrence of the natural fissures in the coal reservoir becomes a key that determines whether the CBM pool can be formed or not. Based on the principle, the authors have designed a comprehensive method of physical modeling to study the elasticity of the coal matrix block with different coal ranks in the light of the solid to fluid coupling, have established the model of the volume deformation (self-adjusted elastic effect) of the coal reservoir under the condition of the effective stress-adsorption/desorption, and have obtained a correct understanding of the law of the elastic deformation. Afterwards, a new viewpoint, named as the self-closing elastic effect for forming the CBM pool in the high rank reservoir, is put forward, providing an initial point and a scientific basis for a further investigation into its dynamic factors, possible mechanism and role in the CBM energy-balancing system. | QIN Yong FU Xuehai WU Caifang FU Guoyou BU Yingying | 2005 | Chinese Science Bulletin2005，50，S1： | 9 |
| 7 | Au nanorods decorated TiO_(2)nanobelts with enhanced full solar spectrum photocatalytic antibacterial activity and the sterilization file cabinet application显示文摘TiO_(2)photocatalysts have been widely studied and applied for removing bacteria,but its antibacterial efficiency is limited to the ultraviolet(UV)range of the solar spectrum.In this work,we use the gold(Au)nanorods to enhance the visible and near-infrared(NIR)light absorption of TiO_(2)NBs,a typical UV light photocatalyst,thus the enhancement of its full solar spectrum(UV,visible and NIR)photocatalytic antibacterial properties is achieved.Preliminary surface plasmon resonance(SPR)enhancement photocatalytic antibacterial mechanism is suggested.On one hand,transverse and longitudinal SPR of Au NRs is beneficial for visible and NIR light utilization.On the other hand,Au NRs combined with TiO_(2)NBs to form the heterostructure,which can improve the photogenerated carrier separation and direct electron transfer increases the hot electron concentration while Au NRs as the electron channel can well restrain charge recombination.finally produces the high yield of radical oxygen species and exhibits a superior antibacterial efficiency.Furthermore,we design a sterilization file cabinet with Au NR/TiO_(2)NB heterostructures as the photocatalytic coating plates.Our study reveals that Au NR/TiO_(2)NB heterostructure is a potential candidate for sterilization of bacteria and archives protection. | Yingying Qin Yichen Guo Zhangqian Liang Yanjun Xue Xiaoli Zhang Lei Yang Jian Tian | 2021 | Chinese Chemical Letters2021，32，4： | 6 |
| 8 | Thymine DNA glycosylase promotes transactivation of β-catenin/TCFs by cooperating with CBP显示文摘胸腺嘧啶 DNA glycosylase (TDG ) ,开始 G/T 和 G/U 的修理的酶错配,最近被发现 crucialin 胚胎维持 epigenetic 稳定性并且便于活跃 DNA demethylation 的开发。这里,我们在作为 -catenin/TCFs 建筑群的 transcriptional 激活剂发信号的 Wnt 报导 TDG 的 novelrole。我们 TDG 绑在 thetranscriptional 因素家庭 LEF1/TCFs 并且加强的数据表演 -catenin/TCFs transactivation,当 TDG 弄空压制 Wnt3a-stimulatedreporter 活动或目标基因抄写时。下次,我们显示出那 CBP,已知的激活剂,也为在目标倡导者上形成合作建筑群的 TDG functionthrough 被要求。而且,有在人的冒号 cancertissue 的 TDG 层次的举起,并且 TDG 击倒禁止冒号房间的增长。总的来说,我们的结果表明作为新激活剂, TDG 支持 -catenin/TCFs transactivation 并且机能上地在正规 Wnt 发信号与 CBP 合作。 | Yingying Jia Fen Nie Aiying Du Zhangcheng Chen Yuanbo Qin Tao Huang Xiaomin Song Lin Li | 2014 | Journal of Molecular Cell Biology2014，8，3： | 5 |
| 9 | Analysis of Commonly and Specifically Dysregulated Pathways in Three Women Cancers显示文摘Breast, ovarian and endometrial cancer are three most prevalent gynaecological malignancies. Identifying their common and specific biomarkers is significant for cancer prediction and therapy in females. We propose a method to identify dysregulated pathways in cancer through scoring pathways based on the molecular interaction data and genomic data. Commonly and specifically dysregulated pathways are analyzed across the above three female cancers, which have not been studied as a whole to the best of our knowledge. Our results demonstrate that all the three cancers have close relationships with Type II diabetes and cell cycle-related biology processes. Breast cancer is specifically related to immune system while ovarian cancer and endometrial cancer are associated with blood vascular-related systems such as renin-angiotensin system and coagulation system. In addition, dysregulated pathways are used to predict potential driver genes effectively according to their topological structure and biological information. | LIU Xiyang AN Yingying YU Baoguo QIN Guimin | 2018 | Chinese Journal of Electronics2018，27，5： | 4 |
| 10 | Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene显示文摘Detection of pigs epidemic diarrhea virus( PEDV) was conducted on 331 piglets diarrhea fecal samples collected in Nanning,Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction( RT-PCR). The results showed that the positive samples of PEDV were 210 and the positive rate was 63. 44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from Gene Bank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96. 0%- 99. 6% and 94. 3%- 99. 6%,respectively. The genetic variation analysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Beijing,Anhui,Wuhan,Hebei and Guangdong from 2010 to 2013,and which were far from that of the Chinese early isolates CH / S( Gen Bank number: JN547228),vaccine strain CV777( Gen Bank number: AF353511) and Attenuated DR13( Gen Bank number: JQ023162). Indicating that the PEDV strains prevalent in Guangxi in recent years showed significant variation with the early isolates. | Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning Jiang Dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，1： | 3 |
| 11 | Si-doping bone composite based on protein template- mediated assembly for enhancing bone regeneration显示文摘 | Qin YANG Yingying DU Yifan WANG Zhiying WANG Jun MA Jianglin WANG Shengmin ZHANG | 2017 | Frontiers of Materials Science2017，11，2： | 3 |
| 12 | Homocysteine aggravates cerebral hemorrhage in rats: Correlation with oxygen-free radical production and cell apoptosis in tissue surrounding hematoma显示文摘BACKGROUND:Previous studies have demonstrated that homocysteine is an independent risk factor for ischemic stroke,as determined by detection of apoptosis and oxygen-free radical scavengers following cerebral ischemia.However,the mechanisms of homocysteine remain unclear.Several reports have addressed the effects of homocysteine on ischemic stroke.OBJECTIVE:To analyze the effects of homocysteine on apoptosis,intracellular superoxide dismutase(SOD) activity,and malondialdehyde content in tissue surrounding hematoma in rats with cerebral hemorrhage,and to determine the action pathway of malondialdehyde following cerebral hemorrhage.DESIGN,TIME AND SETTING:The randomized,controlled,animal experiment was performed at the Laboratory of Molecular Biology,Hospital Affiliated to Luzhou Medical College,China from April 2007 to April 2008.MATERIALS:In situ apoptosis detection kit(Roche,Mannheim,Germany),SOD detection kit and malondialdehyde detection kit(Nanjing Jiancheng Bioengineering Institute,China),and homocysteine(Sigma,St Louis,MO,USA) were used in the present study.METHODS:A total of 75 Sprague Dawley rats were equally and randomly assigned to sham surgery,model,and homocysteine groups.Autologous blood was infused into the caudate putamen of rats to establish models of cerebral hemorrhage in model and homocysteine groups.Homocysteine was injected directly into the brain through the skull at the hematoma hemisphere at 30 minutes after model induction in the homocysteine group.MAIN OUTCOME MEASURES:At 6,12,24,and 72 hours,as well as 1 week,post-surgery,neurological deficits were observed in each group.Brain water content was measured using the dry-wet weight method.Cell apoptosis in tissue surrounding the hematoma was detected utilizing terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).SOD activity and malondialdehyde content in tissue surrounding the hematoma were respectively measured using the xanthine oxidase and thiobarbituric acid methods.RESULTS:Neurological function was similar between model and homocysteine groups following cerebral hemorrhage(P > 0.05).Brain water content was increased at 12 hours post-surgery,peaked at 3 days,and remained unchanged at 7 days in the model group.Brain edema was not significantly aggravated following homocysteine intervention(P > 0.05),but SOD activity significantly decreased and malondialdehyde content significantly increased(P < 0.05).The number of apoptotic cells increased in rats with cerebral hemorrhage at 12 hours(P < 0.05),and numbers peaked at 72 hours following model establishment(P < 0.05).The time of peak value was identical between model and homocysteine groups.Brain water content was negatively associated with SOD activity(rmodel group =-0.448,P < 0.05;rhomocysteine group =-0.612,P < 0.05),but was positively associated with malondialdehyde content(rmodel group = 0.542,P < 0.05;rhomocysteine group = 0.684,P < 0.05) in brain tissues surrounding the hematoma following surgery in model and homocysteine groups.CONCLUSION:Homocysteine aggravates neurological dysfunction and brain edema in rats with cerebral hemorrhage.The mechanisms of action are likely associated with production of oxygen-free radical and cellular apoptosis following cerebral hemorrhage. | Yingying Liu Xinjun Li Chun Wang Chun Chen Ping Liu Qin Feng Duanxiu Liao Xiaogang Li | 2010 | Neural Regeneration Research2010，5，13： | 3 |
| 13 | An injectable micelle-hydrogel hybrid for localized and prolonged drug delivery in the management of renal fibrosis显示文摘Localized delivery,comparing to systemic drug administration,offers a unique alternative to enhance efficacy,lower dosage,and minimize systemic tissue toxicity by releasing therapeutics locally and specifically to the site of interests.Herein,a localized drug delivery platform ('plum?pudding'structure) with controlled release and long-acting features is developed through an injectable hydrogel ('pudding') crosslinked via self-assembled triblock polymeric micelles ('plum') to help reduce renal interstitial fibrosis.This strategy achieves controlled and prolonged release of model therapeutics in the kidney for up to three weeks in mice.Following a single injection,local treatments containing either anti-inflammatory small molecule celastrol or anti-TGFb antibody effectively minimize inflammation while alleviating fibrosis via inhibiting NF-k B signaling pathway or neutralizing TGF-b1 locally.Importantly,the micelle-hydrogel hybrid based localized therapy shows enhanced efficacy without local or systemic toxicity,which may represent a clinically relevant delivery platform in the management of renal interstitial fibrosis. | Xianyan Qin Yingying Xu Xu Zhou Tao Gong Zhi-Rong Zhang Yao Fu | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 2 |
| 14 | Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy显示文摘Mitochondria as a signaling platform play crucial roles in deciding cell fate.Many classic anticancer agents are known to trigger cell death through induction of mitochondrial damage.Mitophagy,one selective autophagy,is the key mitochondrial quality control that effectively removes damaged mitochondria.However,the precise roles of mitophagy in tumorigenesis and anticancer agent treatment remain largely unclear.Here,we examined the functional implication of mitophagy in the anticancer properties of magnolol,a natural product isolated from herbal Magnolia officinalis.First,we found that magnolol induces mitochondrial depolarization,causes excessive mitochondrial fragmentation,and increases mitochondrial reactive oxygen species(mtROS).Second,magnolol induces PTEN-induced putative kinase protein 1(PINK1)-Parkin-mediated mitophagy through regulating two positive feedforward amplification loops.Third,magnolol triggers cancer cell death and inhibits neuroblastoma tumor growth via the intrinsic apoptosis pathway.Moreover,magnolol prolongs the survival time of tumor-bearing mice.Finally,inhibition of mitophagy by PINK1/Parkin knockdown or using inhibitors targeting different autophagy/mitophagy stages significantly promotes magnolol-induced cell death and enhances magnolol's anticancer efficacy,both in vitro and in vivo.Altogether,our study demonstrates that magnolol can induce autophagy/mitophagy and apoptosis,whereas blockage of autophagy/mitophagy remarkably enhances the anticancer efficacy of magnolol,suggesting that targeting mitophagy may be a promising strategy to overcome chemoresistance and improve anticancer therapy. | Yancheng Tang Liming Wang Tao Yi Jun Xu Jigang Wang Jiang-Jiang Qin Qilei Chen Ka-Man Yip Yihang Pan Peng Hong Yingying Lu Han-Ming Shen Hu-Biao Chen | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 2 |
| 15 | Deficiency of the G protein Gαq ameliorates experimental autoimmune encephalomyelitis with impaired DC-derived IL-6 production and Th17 differentiation显示文摘许多 G 联合蛋白质的受体(GPCR ) 被报导涉及多重硬化(MS ) 的致病,并且 ~40% 所有识别 GPCR 依靠 Gq/11 G 蛋白质家庭刺激肌醇类脂化合物发信号。然而,在 MS 致病的 G 子单元的功能仍然是未知的。在这研究,我们试图在试验性的自体免疫的脑脊髓炎(EAE ) 的致病决定 Gq 的角色, MS 的一个著名老鼠模型。我们发现与野类型的老鼠相比, Gq 大美人老鼠展出了不太严重的 EAE 症状,与更低的临床的分数,减少的白血球渗入和不太广泛的 demyelination。而且, Th17 房间的一个显著地更低的百分比,在 MS 致病的关键播放器之一,在 Gq 大美人 EAE 老鼠被观察。在 vitro 的研究证明在 CD4 + T 房间的 Gq 的那缺乏直接损害了 Th17 区别。另外, Gq 的缺乏显著地损害了表明 DC 涉及减少的 IL-6 生产的小径的导出 DC 的 IL-6 生产,因此禁止的 Th17 区别和 Gq-PLC-PKC 和 Gq-MAPKs。在摘要,我们的数据在调整 Th17 区别和 MS 致病加亮 Gq 的关键角色。 | Weiming Lai Yingying Cai Jinfeng Zhou Shuai Chen Chaoyan Qin Cuixia Yang Junling Liu Xin Xie Changsheng Du | 2017 | Cellular & Molecular Immunology2017，14，6： | 2 |
| 16 | Recent developments in regenerative ophthalmology显示文摘Regenerative medicine(RM)is one of the most promising disciplines for advancements in modern medicine,and regenerative ophthalmology(RO)is one of the most active fields of regenerative medicine.This review aims to provide an overview of regenerative ophthalmology,including the range of tools and materials being used,and to describe its application in ophthalmologic subspecialties,with the exception of surgical implantation of artificial tissues or organs(e.g.,contact lens,artificial cornea,intraocular lens,artificial retina,and bionic eyes)due to space limitations.In addition,current challenges and limitations of regenerative ophthalmology are discussed and future directions are highlighted. | Ye Shen He Shen Dongyu Guo Xinghuai Sun Yuan Sun Nan Hong Xiawei Wang Chen Xie Yuan Zhao Qin He Le Jin Yingying Wen Bo Jiang Chenying Yu Miaomiao Zhu Feng Cai Jianwu Dai | 2020 | Science China(Life Sciences)2020，63，10： | 2 |
| 17 | Hepatitis C virus infection in China: an emerging public health issue显示文摘 | Qianqian Qin M. Kumi Smith Lu Wang Yingying Su Liyan Wang Wei Guo Lan Wang Yan Cui Ning Wang | 2015 | J Viral Hepat2015，，3： | 1 |
| 18 | Recent climate changes over the Tibetan Plateau and their impacts on energy and water cycle: A review显示文摘 | Kun Yang Hui Wu Jun Qin Changgui Lin Wenjun Tang Yingying Chen | 2014 | Global and Planetary Change2014，，： | 1 |
| 19 | Study on the effects of the different polar group of EPA-enriched phospholipids on the proliferation and apoptosis in 95D cells显示文摘EPA-enriched phosphatidylcholine(EPA-PC)and EPA-enriched phosphatidylethanolamine(EPA-PE)are newly identified marine phospholipids.The polar group of phospholipids is known to influence EPA-phospholipid activity.However,the differences in anti-tumor effects between EPA-PC and EPA-PE have not been reported.In this study,we evaluated the effects of two forms of EPA on the proliferation and apoptosis in the lung-cancer cell line 95D as well as possible molecular mechanisms.Our results showed that EPA-PC effectively inhibited proliferative activity and promoted apoptosis of 95D cells in a dose-dependent manner,while EPA-PE had no effect on cell proliferation,although it slightly promoted apoptosis.Western blot results showed that EPA-PC and EPA-PE upregulated the expression of PPARy,RXRoc,and PTEN,and downregulated the PI3K/AKT signaling pathway.Furthermore,EPA-PC and EPA-PE induced the expression of the pro-apoptotic gene,Bax,and reduced the expression of the anti-apoptotic gene,Bcl-xl.Additionally,EPA-PC and EPA-PE promoted the release of cytochrome c and activated the apoptotic enzyme-cleaved caspase-3.These data suggest that the anti-tumor effect of EPA-phospholipids may be exerted via a PPARy-related mechanism.EPA-PC was more efficacious as compared to EPA-PE,which might be due to the different polar groups of phospholipids. | Yao Guo Qin Zhao Yingying Tian Yuanyuan Liu Ziyi Yan Changhu Xue Jingfeng Wang | 2021 | Marine Life Science & Technology2021，3，4： | 1 |
| 20 | Brain organoid-on-chip system to study the effects of breast cancer derived exosomes on the neurodevelopment of brain显示文摘Early human brain development can be affected by multiple prenatal factors that involve chemical exposures in utero,maternal health characteristics such as psychiatric disorders,and cancer.Breast cancer is one of the most common cancers worldwide arising pregnancy.However,it is not clear whether the breast cancer might influence the brain development of fetus.Exosomes secreted by breast cancer cells play a critical role in mediating intercellular communication and interplay between different organs.In this work,we engineered human induced pluripotent stem cells(hiPSCs)-derived brain organoids in an array of micropillar chip and probed the influences of breast cancer cell(MCF-7)derived-exosomes on the early neurodevelopment of brain.The formed brain organoids can recapitulate essential features of embryonic human brain at early stages,in terms of neurogenesis,forebrain regionalization,and cortical organization.Treatment with breast cancer cell derived-exosomes,brain organoids exhibited enhanced expression of stemness-related marker OCT4 and forebrain marker PAX6.RNA-seq analysis reflected several activated signaling pathways associated with breast cancer,medulloblastoma and neurogenesis in brain organoids induced by tumor-derived exosomes.These results suggested that breast cancer cell-derived exosomes might lead to the impaired neurodevelopment in the brain organoids and the carcinogenesis of brain organoids.It potentially implies the fetus of pregnant women with breast cancer has the risk of impaired neurodevelopmental disorder after birth. | Kangli Cui Wenwen Chen Rongkai Cao Yingying Xie Peng Wang Yunsong Wu Yaqing Wang Jianhua Qin | 2022 | Cell Regeneration2022，11，1： | 1 |
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