|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Characterization of M2 antibodies in asymptomatic Chinese population显示文摘AIM: To investigate the presence of M2 antibodies specific for pdmary biliary cirrhosis (PBC) in asymptomatic Chinese and identify patients with early PBC.METHODS: Enzyme-linked immunosorbent assay (ElISA)tests for M2 antibodies to recombinant protein were performed in 5 011 subjects (age range, 26-85 years; mean age: 45.81±15.02 years) who took an annual physical examination. M2-positive subjects were further analyzed for immunoglobulin (Ig) classes and subclasses of M2 antibodies.Clinical, biochemical and immunological data were obtained for M2-positive subjects. In addition, ultrasonography (US)or endoscopic retrograde cholangio-pancreatography (ERCP)was performed to exclude any disorders other than PBC.RESULTS: M2 antibodies were detected in 8 (0.16%) of the 5 0LL subjects studied. Of the 8 subjects, 7 were female and 1 was male (age range: 40-74 years). An unexplained increase of serum alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (γ-GT) values, often to striking levels,was detected in 4 M2-positive subjects, 3 of them accorded with the diagnostic criteria recommended by the American Association for the Study of Liver Diseases, even though they had no symptoms of PBC (such as fatigue, pruritus or jaundice).Liver biopsy was performed in two M2-positive subjects and the histology was compatible with PBC in both cases.CONCLUSION: Our data, while not assessing the true prevalence of asymptomatic PBC in the general population,suggest that asymptomatic PBC is much more common in China than has been supposed. | Xiao-HuaJiang Ren-QianZhong Xiao-YunFan YinHu FengAn Jian-WenSun Xian-TaoKong | 2003 | World Journal of Gastroenterology2003，9，9： | 20 |
| 2 | Construction and expression of a humanized M_2 autoantigen trimer and its application in the diagnosis of primary biliary cirrhosis显示文摘AIM: To construct and express a humanized M2 autoantigen trimer designated as BPO and to apply it in the diagnosis of primary biliary cirrhosis (PBC).
METHODS: cDNA fragments encoding M2-reactive epitopes of pyruvate dehydrogenase complex Ez (PDCE2), branched chain 2-oxo-acid dehydrogenase complex E2 (BCOADC-E2) and 2-oxo-glutarate dehydrogenase complex E2 (OGDC-E2) were amplified with PCR using total RNA extracted from human peripheral mononuclear blood cells. The fragments were cloned into the plasmid vector pQE-30 and then transferred into E. coliM15 (pREP4) for expression, which was induced by isopropylthio-β-Dgalactoside. The expressed recombinant BPO protein was demonstrated by SDS-PAGE, Western-blotting and Immunoabsorption test, its antigenic reactivity and specificity were identified with seven M2-positive sera confirmed at Euroimmun Research Center (Germany).Using the purified BPO, M2 antibodies in sera from patients with PBC and other liver related diseases were detected with ELISA.
RESULTS: The expressed BPO was observed with both antigenic reactivity and specificity of M2 autoantigens. The determination of M2 antibodies by BPO with ELISA was more sensitive than using the Euroimmun's kit with the coefficients of variation less than 10 % in both interassay and intraassay.With the newly established method, M2 antibodies were found in 100 % (20/20) of patients with PBC. Six cases of liver disease with unknown etiology and 1 patient with drug induced liver injury had detectable levels of serum M2antibodies. There were also 2 patients with autoimmune cholangitis and 1 with autoimmune hepatitis showing M2-antibody positive.
CONCLUSION: Compared with the routine immunofluorescenoe assay and commercially available assay kit using porcine heart mitochondrial protein as the antigen, the detection system established in the present study shows higher sensitivity and specificity and may be used as a powerful tool for the diagnosis of PBC. | Xiao-HuaJiang Ren-QianZhong Sheng-QianYu YinHu Weng-WengLi Xian-TaoKong | 2003 | World Journal of Gastroenterology2003，9，6： | 17 |
| 3 | Statistical and frequency analysis of the pressure fluctuation in a fluidized bed of non-spherical particles显示文摘In this paper,the pressure fluctuation in a fluidized bed was measured and processed via standard deviation and power spectrum analysis to investigate the dynamic behavior of the transition from the bubbling to turbulent regime.Two types[Geldart B and D) of non-spherical particles,screened from real bed materials,and their mixture were used as the bed materials.The experiments were conducted in a semiindustrial testing apparatus.The experimental results indicated that the fluidization characteristics of the non-spherical Geldart D particles differed from that of the spherical particles at gas velocities beyond the transition velocity U_c.The standard deviation of the pressure fluctuation measured in the bed increased with the gas velocity,while that measured in the plenum remained constant.Compared to the coarse particles,the fine particles exerted a stronger influence on the dynamic behavior of the fluidized bed and promoted the fluidization regime transition from bubbling toward turbulent.The power spectrum of the pressure fluctuation was calculated using the auto-regressive(AR) model;the hydrodynamics of the fluidized bed were characterized by the major frequency of the power spectrum of the pressure fluctuation.By combining the standard deviation analysis,a new method was proposed to determine the transition velocity U_k via the analysis of the change in the major frequency.The first major frequency was observed to vary within the range of 1.5 to 3 Hz. | Honghao He Xiaofeng Lu Wei Shuang Quanhai Wang Yinhu Kang Liyun Yan Xuanyu Ji Guangyu Luo Hai Liu | 2014 | Particuology2014，12，5： | 6 |
| 4 | An experimental method for measuring friction behaviors of linear rolling guides显示文摘Friction behaviors of linear rolling guides(LRGs)are determined by many factors and are crucial for precision positioning.It is difficult to create an expression to describe the friction behaviors exactly and completely.A new experimental method for measuring the friction behaviors of one LRG individually was developed,and linear motor direct drive technology is utilized in the experiment setup.The position signal is acquired by a grating,and the friction of the LRG is deduced by the product of the mass of the moving part and its acceleration.In the experiment,the LRG can move with the friction only,and the friction transition from normal rolling to prerolling stage was detected.The asperities’deformations were utilized to explain it.Hysteresis loops and internal loops were measured.The results indicate the non-local memory of the LRG,and the historical trajectory have an important impact on the friction behaviors of LRGs in the pre-rolling stage. | Yinhu Xi Yuan Zhou Wei Zhang Junhong Mao | 2014 | Chinese Science Bulletin2014，59，29： | 2 |
| 5 | Clinical Significance of hTERC Gene Detection in Exfoliated Cervical Epithelial Cells for Cervical Lesions显示文摘 | Xiuhui Zheng Peihe Liang Yingru Zheng Ping Yi Qiang Liu Jian Han Yinhu Huang Yuanguo Zhou Jianxin Guo Li Li | 2013 | International Journal of Gynecological Cancer2013，，5： | 1 |
| 6 | Ozonation as an efficient pretreatment method to alleviate reverse osmosis membrane fouling caused by complexes of humic acid and calcium ion显示文摘Humic acid has been considered as one of the most significant sources in feed water causing organic fouling of reverse osmosis (RO) membranes, but the relationship between the fouling behavior of humic acid and the change of its molecular structure has not been well developed yet. In this study, the RO membrane fouling behavior of humic acid was studied systematically with ozonation as a pretreatment method to control RO membrane fouling. Furthermore, the effect of ozone on the structure ofhumic acid was also explored to reveal the mechanisms. Humic acid alone (10-90 mg/L, in deionized water) was found not to cause obvious RO membrane fouling in 45-h operation. However, the presence of Ca^2+ aggravated significantly the RO membrane fouling caused by humic acid, with significant flux reduction and denser fouling layer on RO membrane, as it was observed by scanning electron microscope (SEM) and atomic force microscope (AFM). However, after the pretreatment by ozone, the influence of Ca^2+ was almost eliminated. Further analysis revealed that the addition of Ca^2+ increased the particle size of humic acid solution significantly, while ozonation reduced the SUVA254, particle size and molecular weight of the complexes of humic acid and Ca^2+(HA-Ca^2+ complexes). According to these results and literature, the bridge effect of Ca^2+ aggregating humic acid molecules and the cleavage effect of ozone breaking HA-Ca^2+ complexes were summarized. The change of the structure of humic acid under the effect of Ca^2+ and ozone is closely related to the change of its membrane fouling behavior. | Xuehao Zhao Yinhu Wu Xue Zhang Xin Tong Tong Yu Yunhong Wang Nozomu Ikuno Kazuki Ishii Hongying Hu | 2019 | Frontiers of Environmental Science & Engineering2019，13，4： | 1 |
| 7 | Longitudinal virological changes and underlying pathogenesis in hospitalized COVID-19 patients in Guangzhou,China显示文摘Prolonged viral RNA shedding and recurrence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in coronavirus disease 2019(COVID-19)patients have been reported.However,the clinical outcome and pathogenesis remain unclear.In this study,we recruited 43 laboratory-confirmed COVID-19 patients.We found that prolonged viral RNA shedding or recurrence mainly occurred in severe/critical patients(P<0.05).The average viral shedding time in severe/critical patients was more than 50 days,and up to 100 days in some patients,after symptom onset.However,chest computed tomography gradually improved and complete absorption occurred when SARS-CoV-2 RT-PCR was still positive,but specific antibodies appeared.Furthermore,the viral shedding time significantly decreased when the A1,430G or C12,473T mutation occurred(P<0.01 and FDR<0.01)and increased when G227A occurred(P<0.05 and FDR<0.05).High IL1R1,IL1R2,and TNFRSF21 expression in the host positively correlated with viral shedding time(P<0.05 and false discovery rate<0.05).Prolonged viral RNA shedding often occurs but may not increase disease damage.Prolonged viral RNA shedding is associated with viral mutations and host factors. | Zhengtu Li Yinhu Li Ruilin Sun Shaoqiang Li Lingdan Chen Yangqing Zhan Mingzhou Xie Jiasheng Yang Yanqun Wang Airu Zhu Guoping Gu Le Yu Shuaicheng Li Tingting Liu Zhaoming Chen Wenhua Jian Qian Jiang Xiaofen Su Weili Gu Liyan Chen Jing Cheng Jincun Zhao Wenju Lu Jinping Zheng Shiyue Li Nanshan Zhong Feng Ye | 2021 | Science China(Life Sciences)2021，64，12： | 1 |
| 8 | The Brauer group of Yetter-Drinfeld module algebras 显示文摘 | CAENEPEEL S VAN O F ZHANG Yinhu | 1997 | Transactions of the A- merican Mathematical Society1997，349，9： | 1 |
| 9 | A Method to Estimate DCB of COMPASS Satellite Based on Global Ionosphere Map 显示文摘 | Jiao Wenhai Chang Jianggeng Ma Yinhu | 2012 | Lecture Notes in Electrical Engineering2012，，159： | 1 |
| 10 | Investigations on heat treatment of a high-speed steel roll显示文摘 | Fu Hanguang Qu Yinhu Xing Jiandong | 2008 | Journal of Materials Engineering and Performance2008，17，4： | 1 |
| 11 | Effect of chlorinationand ultraviolet disinfection on tetA : mediated tetracycline resistance ofEscherichia coli显示文摘 | HUANG Jingjing HU Hongying WU Yinhu | 2013 | Chemosphere2013，90，8： | 1 |
| 12 | Investigations on heat treatment of a high-speed steel roll 显示文摘 | Fu Hanguang Qu Yinhu Xing Jiandong Zhi Xiaohui Jiang Zhiqiang Li Mingwei Zhang Yi | 2008 | Journal of Materials Engineering and Performance2008，17，4： | 1 |
| 13 | Modelling the thresholds of nitrogen/phosphorus concentration and hydraulic retention time for bloom control in reclaimed water landscape显示文摘The risks posed by algal blooms caused by nitrogen and phosphorus in reclaimed water used in urban water landscapes need to be carefully controlled.In this study,the combined effects of the nitrogen and phosphorus concentrations and the light intensity and temperature on the specific growth rates of algae were determined using Monod,Steele,and Arrhenius models,then an integrated algal growth model was developed.The algae biomass,nitrogen concentration,and phosphorus concentration mass balance equations were used to establish a new control model describing the nitrogen and phosphorus concentration and hydraulic retention time thresholds for algal blooms.The model parameters were determined by fitting the models to data acquired experimentally.Finally,the control model and numerical simulations for six typical algae and mixed algae under standard conditions were used to determine nitrogen/phosphorus concentration and hydraulic retention time thresholds for landscape water to which reclaimed water is supplied(i.e.,for a reclaimed water landscape). | Keying Song Shufeng Zhu Yun Lu Guohua Dao Yinhu Wu Zhuo Chen Shengnan Wang Junhan Liu Wenguang Zhou Hong-Ying Hu | 2022 | Frontiers of Environmental Science & Engineering2022，16，10： | 1 |
| 14 | Significant increase of assimilable organic carbon(AOC)levels in MBR effluents followed by coagulation,ozonation and combined treatments:Implications for biostability control of reclaimed water显示文摘As water reuse development has increased,biological stability issues associated with reclaimed water have gained attention.This study evaluated assimilable organic carbon(AOC)in effluents from a full-scale membrane biological reactor(MBR)plant and found that they were generally stable over one year(125-216µg/L),with slight increases in warmer seasons.After additional tertiary treatments,the largest increases in absolute and specific AOCs were detected during ozonation,followed by coagulation-ozonation and coagulation.Moreover,UV254 absorbance is known to be an effective surrogate to predict the AOC changes during ozonation.Applying coagulation prior to ozonation of MBR effluents for removal of large molecules was found to reduce the AOC formation compared with ozonation treatment alone.Finally,the results revealed that attention should be paid to seasonal variations in influent and organic fraction changes during treatment to enable sustainable water reuse. | Xiaojie Shi Zhuo Chen Yun Lu Qi Shi Yinhu Wu Hong-Ying Hu | 2021 | Frontiers of Environmental Science & Engineering2021，15，4： | 1 |
| 15 | A novel semiconductor-sensitized solar cell based on P3HT@CdS@TiO 2 core-shell nanotube array显示文摘 | Yanzhong Hao Yinhu Cao Bao Sun Yingpin Li Yanhui Zhang Dongsheng Xu | 2012 | Solar Energy Materials and Solar Cells2012，，： | 1 |
| 16 | Comparison of next-gen-eration sequencing systems显示文摘 | Liu Lin Li Yinhu Li Siliang | 2012 | J Biomed Biotechnol2012，10，1155： | 1 |
| 17 | Comparison of Next-Generation Sequencing systems显示文摘 | LIN L YINHU L SILIANG L | 2012 | Journal of Biomedicine and Biotechnology2012，，12： | 1 |
| 18 | The Study on Method Choice in the Supply of Rural Public Goods显示文摘 | Yinhu Wang Lin Yu | 2010 | International Journal of Business and Management2010，，56： | 1 |
| 19 | Effect of cerium on the as-cast microstrueture of a hypereutectic high chromium cast iron显示文摘 | Qu Yinhu Xing Jiandong Zhi Xiaohui | 2008 | Material Letters2008，62，1617： | 1 |
| 20 | A Method to Estimate DCB of COMPASS Satellite Based on Global Ionosphere Map显示文摘 | Jiao Wenhai Chang Jianggeng Ma Yinhu | 2012 | Lecture Notes in Electrical Engineering2012，，159： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员