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| 1 | Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo显示文摘Silk fibroin(SF)has been used extensively in the biomedical field including tissue engineering for the generation of artificial bones,skins or ligaments.We have previously reported on good in vitro biocompatibility of SF fibers with peripheral nerve tissues and cells.In the present study,we developed a novel design of the SF-based artificial nerve graft(SF graft)which was composed of a SF-nerve guidance conduit(NGC)inserted with SF fibers.MTT assay was performed to determine the cytotoxicity of the SF-NGC extract fluid on the cultured L929 cells derived from an immortalized mouse fibroblast cell line.In addition,this SF graft was implanted into adult rats for bridging a 10-mm long sciatic nerve defect.The following-up experiments at initial stage(1-4 week)of nerve regeneration including routine blood tests and histochemical investigation were conducted to evaluate the in vivo biocompatibility of the SF graft with peripheral nerves.The results demonstrated that the SF-NGC graft was biocompatible with the surrounding tissues and cells due to its low inflammatory potential with a grade 0 under the U.S.Pharmacopeia guidelines and it was generally suitable to a certain degree for bridging peripheral nerve defects in virtue of supporting Schwann cell adherence,expansion and migration.Therefore the SF graft is a promising alternative to classical autografts for peripheral nerve repair. | Chen Xuemei Yang Yumin Wu Jian Zhao Yahong Ding Fei Gu Xiaosong | 2007 | Progress in Natural Science:Materials International2007，17，9： | 5 |
| 2 | Reduced function and disassembled microtubules of cultured cardiomyocytes in spaceflight显示文摘Lack of gravity during spaceflight has profound effects on cardiovascular system, but little is known about how the cardiomyocytes respond to microgravity. In the present study, the effects of spaceflight on the structure and function of cultured cardiomyocytes were reported. The primary cultures of neo- natal rat cardiomyocytes were carried on Shenzhou-6 spacecraft and activated at 4 h in orbit. 8 samples were fixed respectively at 4, 48 and 96 h after launching for immunofluorescence of cytoskeleton, and 2 samples remained unfixed to analyze contractile and secretory functions of the cultures. Ground sam- ples were treated in our laboratory in parallel. After 115 h spaceflight, video recordings displayed that the number of spontaneous beating sites in flown samples decreased significantly, and the cells in the beating aggregate contracted in fast frequency without synchrony. Radioimmunoassay of the medium showed that the atrial natriuretic peptide secreted from flown cells reduced by 59.6%. Confocal images demonstrated the time-dependant disassembly of mirotubules versus unchanged distribution and or- ganization of microfilaments. In conclusion, above results indicate reduced function and disorganized cytoskeleton of cardiomyocytes in spaceflight, which might provide some cellular basis for further investigations to probe into the mechanisms underlying space cardiovascular dysfunction. | YANG Fen LI YingHui DING Bai NIE JieLin WANG HongHui ZHANG XiaoYou WANG ChunYan LING ShuKuan NI ChengZhi DAI ZhongQuan TAN YingJun WAN YuMin | 2008 | Chinese Science Bulletin2008，53，8： | 5 |
| 3 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 4 | Evolution of microstructures and optical properties of gadolinium oxide with oxygen flow rate and annealing temperature显示文摘In this study, the effects of oxygen flow rate and annealing temperature on Gd_2 O_3 structures and optical properties were systematically analyzed. Gd_2 O_3 films were deposited on both quartz and ZnS substrates by magnetron sputtering and then annealed under vacuum at 700, 800 and 900℃, Restructure and phase transformation from cubic to monoclinic occur at different temperatures depending on the oxygen flow rate. The optical band gap, which is more sensitive to the annealing temperature than oxygen flow rate changes from 5.32 to 5.65 eV. The refractive index is approximately 1.75 at 550 nm and is adjustable by the oxygen flow rate. The transmittance of the ZnS substrate with Gd_2 O_3 film exceeds 80% and reaches82% at the 7.5-9.5 μm range. When ZnS is coated on both sides, the transmittance is increased to approximately 90%. Our results indicate that Gd_2 O_3 films are promising new candidates for anti-reflective coatings in the infrared region. | Zhenhuai Yang Lei Yang Bing Dai Pei Lei Shuai Guo Peng Wang Qiang Wang Yujie Ding Yumin Zhang Jiecai Han Jiaqi Zhu | 2019 | Journal of Rare Earths2019，37，4： | 2 |
| 5 | The promotion of peripheral nerve regeneration by chitooligosaccharides in the rat nerve crush injury model显示文摘 | Maorong Jiang Xiaoming Zhuge Yumin Yang Xiaosong Gu Fei Ding | 2009 | Neuroscience Letters2009，，3： | 1 |
| 6 | The diagnostic value of apolipoprotein E in malignant pleural effusion associated with non-small cell lung cancer显示文摘 | Yumin Wang Zengqiang Chen Jie Chen Jingye Pan Wenhui Zhang Qinshi Pan Hongyan Ding Xiaomei Lin Xiushu Wen Yangyang Li Qing H. Meng | 2013 | Clinica Chimica Acta2013，，： | 1 |
| 7 | Enhanced bacterial inhibition activity of layer-by-layer structured polysaccharide film-coated cellulose nanofibrous mats via addition of layered silicate显示文摘 | Hongbing Deng Xiaoying Wang Pu Liu Bin Ding Yumin Du Guoxiang Li Xianwen Hu Jianhong Yang | 2010 | Carbohydrate Polymers2010，，1： | 1 |
| 8 | Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro显示文摘 | Yumin Yang Xuemei Chen Fei Ding Peiyun Zhang Jie Liu Xiaosong Gu | 2006 | Biomaterials2006，，9： | 1 |
| 9 | Cardioprotective effects of saponins from Panax japonicus on acute myocardial ischemia against oxidative stress-triggered damage and cardiac cell death in rats显示文摘 | Haibo He Jia Xu Yuanqing Xu Changcheng Zhang Hongwu Wang Yumin He Ting Wang Ding Yuan | 2011 | Journal of Ethnopharmacology2011，，1： | 1 |
| 10 | The diagnostic value of apolipoprotein E in malignant pleural effusion associated with non-small cell lung cancer显示文摘 | Yumin Wang Zengqiang Chen Jie Chen Jingye Pan Wenhui Zhang Qinshi Pan Hongyan Ding Xiaomei Lin Xiushu Wen Yangyang Li Qing H. Meng | 2013 | Clinica Chimica Acta2013，，： | 1 |
| 11 | Construction of tissue engineered nerve grafts and their application in periph- eral nerve regeneration显示文摘 | Xiaosong Gu Fei Ding Yumin Yang | 2011 | Neurobiology2011，93，： | 1 |
| 12 | Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro显示文摘 | Yumin Yang Xuemei Chen Fei Ding | 2007 | Biomaterials2007，28，： | 1 |
| 13 | Cardioprotective effects of saponins from Panax japonicus on acute myocardial ischemia against oxidative stress-triggered damage and cardiac cell death in rats显示文摘 | Haibo He Jia Xu Yuanqing Xu Changcheng Zhang Hongwu Wang Yumin He Ting Wang Ding Yuan | 2011 | Journal of Ethnopharmacology2011，，1： | 1 |
| 14 | Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration 显示文摘 | YANG Yumin DING Fei WU Jian | 2007 | Biomaterials2007，28，： | 1 |
| 15 | Breathable,stretchable and adhesive nanofibrous hydrogels as wound dressing materials显示文摘In spite of the great progress of wound healing materials,the development of dressing with moisture permeabil-ity,stretchable capability,and adhesive performance remains a huge challenge.In this study,gelatin-dopamine(GT-DA)nanofiber membrane was prepared by introducing dopamine(DA)into gelatin(GT)via electrospinning followed by in-situ reaction.The effects of DA dosage,crosslinking condition and crosslinking time on the mechanical properties of the fiber membrane were studied systematically.The resultant GT-DA nanofibrous hydrogels exhibited integrated stress strength of 1.96 MPa,good water absorption of 700%,and water vapor transmission(WVT:4.3 kg m^(-2)d^(-1)),which were superior than those of Mepiform■,a commercial adhesive silicone dressing.In addition,Fe^(3+)were introduced into one side of the nanofiber membrane.A stronger adhesion strength of 2.5 KPa on the porcine skin,which was 1.6 times higher than that of Mepiform■was achieved.Our research showed that the prepared nanofibrous hydrogel has potential as wound dressing and could be used in post-traumatic tissue healing engineering.This work presented a type of breathable,stretchable and adhesive nanofibrous hydrogels,which would ensure the stable attachment on wounds during movement as a wound healing material. | Xueting Liu Yumin Liu Jingtao Du Xiaoran Li Jianyong Yu Bin Ding | 2021 | Engineered Regeneration2021，2，1： | 1 |
| 16 | Developmental Temporal Patterns and Molecular Network Features in the Transcriptome of Rat Spinal Cord显示文摘The molecular network features of spinal cord development that are integral to tissue engineering remain poorly understood in placental mammals,especially in terms of their relationships with vital biological processes such as regeneration.Here,using a large-scale temporal transcriptomic analysis of rat spinal cord from the embryonic stage to adulthood,we show that fluctuating RNA expression levels reflect highly active transcriptional regulation,which may initiate spinal cord patterning.We also demonstrate that microRNAs(miRNAs)and transcriptional factors exhibit a mosaic profile based on their expression patterns,while differential alternative splicing events reveal that alternative splicing may be a driving force for the development of the node of Ranvier.Our study also supports the existence of a negative correlation between innate immunity and intrinsic growth capacity.Epigenetic modifications appear to perform their respective regulatory functions at different stages of development,while guanine nucleotidebinding protein(G protein)-coupled receptors(including olfactory receptors(ORs))may perform pleiotropic roles in axonal growth.This study provides a valuable resource for investigating spinal cord development and complements the increasing number of single-cell datasets.These findings also provide a genetic basis for the development of novel tissue engineering strategies. | Jian Yang Lili Zhao Sheng Yi Fei Ding Yumin Yang Yan Liu Yongjun Wang Mei Liu Chengbin Xue Lian Xu Leilei Gong Xinghui Wang Yu Zhang Bin Yu Guo-li Ming Xiaosong Gu | 2021 | Engineering2021，7，11： | 0 |
| 17 | National Report on Space Medicine Progress in 2012–2014显示文摘The application of manned spaceflight makes a great impetus on the development of space medicine. With the deep growth of Chinese manned space, our national space medicine has attained great achievements. In this paper, the basic research of Space Medicine and its application in China during 2012–2014 were briefly reviewed. | LI Yinghui ZHANG Xiaoyou WAN Yumin DING Bai LIU Zhaoxia LING Shukuan BAI Yanqiang CHEN Shanguang LIU Shujuan | 2014 | 空间科学学报2014，34，5： | 0 |
| 18 | National Report on Space Medicine Progress in 2014-2016显示文摘The progress of China Spaceflight makes a great impetus for the development of space medicine.With the developing of China Space Station,the national space medicine has attained great achievements.In this paper,the basic research of Space Medicine and its application in China during 2014—2016 are briefly reviewed. | LI Yinghui ZHANG Xiaoyou WAN Yumin DING Bai LIU Zhaoxia LING Shukuan BAI Yanqiang CHEN Shanguang | 2016 | 空间科学学报2016，36，5： | 0 |
| 19 | Path Choice of Rural Land Transfer in China显示文摘Establishing rural land collective property right system with clear ownership,complete powers and functions,smooth transfer,and strict protection is the basic guarantee to the equal exchange of land elements. In this study,the reform of relevant legal systems to China's rural land transfer was introduced firstly,and then the guiding ideology of China's rural land transfer was proposed. Finally,the ways of practicing rural land transfer in China were discussed. Based on the premise of insisting on and improving the strictest land preservation system,farmers should have the right to occupy,use,and transfer their contracted land,and they can transfer their land contracting and management right in ways of exchange,subcontracting,renting,stock cooperation,and trust. Exploring the ways of equal exchange of land elements according to local conditions is an important political and economic issue concerning the livelihood and development of farmers in China,so it should be conducted under the guidance of the correct ideas to integrate scattered land in China's rural areas,develop moderate-scale management,shorten the income gap between urban and rural areas,and realize balancing development of urban and rural areas in China. | Yumin DING Ye LING Yang WU | 2016 | Asian Agricultural Research2016，8，7： | 0 |
| 20 | ROLE OF DISEASE PROPAGATION IN MIGRATORY BIRD POPULATION显示文摘 | SHUJING GAO YUMIN DING JIANPING XIE | 2012 | International Journal of Biomathematics2012，5，3： | 0 |
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