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| 1 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
| 2 | Impacts of aerosol chemical compositions on optical properties in urban Beijing,China显示文摘The optical properties of aerosols and their chemical composition,including water-soluble ions,organic carbon(OC),and elemental carbon(EC) in PM_(2.5) and PM_(10),were measured from 26 May to 30 June of 2012 at an urban site in Beijing.The daily average concentrations of PM_(2.5) and PM_(10) were 103.2 and 159.6 μg/m^3,respectively.On average,the OC and EC contributed 20.1% and 4.3% ,respectively,to PM_(2.5) and 16.3% and3.9% ,respectively,to PM_(10).Secondary ions(SO_4^(2-),NO_3^-,and NH_4^+) dominated the water-soluble ions and accounted for 57.9% and 62.6% of PM_(2.5) and PM_(10),respectively.The wind dependence of PM_(2.5),OC,SO_4^(2-),and NO_3^- implied that the pollution sources mainly came from south and southeast of Beijing during the summer.The monthly mean values of the scattering coefficient(σ_(sc)) and absorption coefficient(σ_(ab)) at525 nm were 312.9 and 28.7 Mm^(-1),respectively,and the mean single-scattering albedo(ω) was 0.85.The wind dependence of σ_(sc) revealed that this value was mainly influenced by regional transport during the summer,and the relationship between σ_(ab) and wind indicated that a high σ_(ab) resulted from the joint effects of local emissions and regional transport.The reconstructed σ_(sc) that was derived from the revised IMPROVE equation agreed well with the observations.The contribution of different chemical species toσ_(sc) was investigated under different pollution levels,and it was found that secondary inorganic aerosols accounted for a large part of σ_(sc) during pollution episodes(35.7% ),while organic matter was the main contributor to σ_(sc) under clean conditions(33.6% ). | Ping Tian Guangfu Wang Renjian Zhang Yunfei Wu Peng Yan | 2015 | Particuology2015，13，1： | 5 |
| 3 | Label-free fiber nanograting sensor for real-time in situ early monitoring of cellular apoptosis显示文摘The achievement of functional nanomodules for subcellular label-free measurement has long been pursued in order to fully understand cellular functions.Here,a compact label-free nanosensor based on a fiber taper and zinc oxide nanogratings is designed and applied for the early monitoring of apoptosis in individual living cells.Because of its nanoscale dimensions,mechanical flexibility,and minimal cytotoxicity to cells,the sensing module can be loaded in cells for long term in situ tracking with high sensitivity.A gradual increase in the nuclear refractive index during the apoptosis process is observed,revealing the increase in molecular density and the decrease in cell volume.The strategy used in our study not only contributes to the understanding of internal environmental variations during cellular apoptosis but also provides a new platform for nonfluorescent fiber devices for investigation of cellular events and understanding fundamental cell biochemical engineering. | Danran Li Nina Wang Tianyang Zhang Guangxing Wu Yifeng Xiong Qianqian Du Yunfei Tian Weiwei Zhao Jiandong Ye Shulin Gu Yanqing Lu Dechen Jiang Fei Xu | 2022 | Advanced Photonics2022，4，1： | 5 |
| 4 | The microfacies and sedimentary responses to the mass extinction during the Permian-Triassic transition at Yangou Section, Jiangxi Province, South China显示文摘A Permian-Triassic(P-Tr) boundary section of continuous carbonate facies, which well recorded the biotic and environmental processes through the great P-Tr transition in the shallow non-microbialite carbonate facies, has been studied in Yangou, Leping County, Jiangxi Province. The P-Tr sequence is well correlated with the Meishan section according to the conodont biostratigraphy and the excursion of carbon isotopes. A series of high-resolution thin-sections from the P-Tr boundary carbonate rocks at the Yangou section are studied to explore the interrelation between environmental change and biological evolution during the transitional time. Six microfacies have been identified based upon the observation of the thin-sections under a microscope on the grains and matrix and their interrelation. Combined with the data of fossils and carbon isotopes, Microfacies 4(MF-4), coated-grain-bearing foraminifer oolitic sparitic limestone, and Microfacies 6(MF-6), dark shelly micritic limestone, should be the different responses to the two episodes of mass extinction and environmental events that can be correlated throughout South China and even over the world. The oolitic limestone of MF-4 is the first finding from the latest Permian strata in South China and it might be a proxy of an unusual environmental condition of high pCO2, low sulfate concentration and of microbial blooming in the aftermath of the latest Permian mass extinction. The micritic limestone of MF-6 containing rich micro-gastropods and ostracods probably represents the blooming event of disaster taxa in the earliest Triassic environment. The microfacies analysis at the Yangou section can well reveal the episodic process of the biological evolution and environmental change in the shallow non-microbialite carbonate facies throughout the great P-Tr transition, thus the Yangou section becomes an important complement to the Meishan section. | TIAN Li TONG JinNan SUN DongYing XIONG YanLin WANG ChengGang SONG HaiJun SONG HuYue HUANG YunFei | 2014 | Science China Earth Sciences2014，57，9： | 4 |
| 5 | printed electrode immunosensors for Schistosoma japonicum显示文摘 | ZENG Shaohua TIAN Zhi CHE Hongli YANG Huihui CHEN Xiuchun FENG Qimei ZHOU Yunfei ZHANG Shuju WU Zhaoyang WANG Shiping | 2012 | 中南大学学报(医学版)2012，37，6： | 3 |
| 6 | TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production of type I interferon by recruiting GSK3β to TBK1显示文摘 | Yunfei Qin Qingxiang Liu Shuo Tian Weihong Xie Jun Cui Rong-Fu Wang | 2016 | Cell Research2016，26，5： | 3 |
| 7 | Predicting the grades of Astragali radix using mass spectrometrybased metabolomics and machine learning显示文摘Astragali radix(AR,the dried root of Astragalus)is a popular herbal remedy in both China and the United States.The commercially available AR is commonly classified into premium graded(PG)and ungraded(UG)ones only according to the appearance.To uncover novel sensitive and specific markers for AR grading,we took the integrated mass spectrometry-based untargeted and targeted metabolomics approaches to characterize chemical features of PG and UG samples in a discovery set(n=16 batches).A series of five differential compounds were screened out by univariate statistical analysis,including arginine,calycosin,ononin,formononetin,and astragalosideⅣ,most of which were observed to be accumulated in PG samples except for astragalosideⅣ.Then,we performed machine learning on the quantification data of five compounds and constructed a logistic regression prediction model.Finally,the external validation in an independent validation set of AR(n=20 batches)verified that the five compounds,as well as the model,had strong capability to distinguish the two grades of AR,with the prediction accuracy>90%.Our findings present a panel of meaningful candidate markers that would significantly catalyze the innovation in AR grading. | Xinyue Yu Jingxue Nai Huimin Guo Xuping Yang Xiaoying Deng Xia Yuan Yunfei Hua Yuan Tian Fengguo Xu Zunjian Zhang Yin Huang | 2021 | Journal of Pharmaceutical Analysis2021，11，5： | 2 |
| 8 | Dipeptidyl Peptidase 4 Inhibitor Sitagliptin Protects Endothelial Function in Hypertension Through a Glucagon–Like Peptide 1–Dependent Mechanism显示文摘 | Limei Liu Jian Liu Wing Tak Wong Xiao Yu Tian Chi Wai Lau Yi-Xiang Wang Gang Xu Yunfei Pu Zhiming Zhu Aimin Xu Karen S.L. Lam Zhen Yu Chen Chi Fai Ng Xiaoqiang Yao Yu Huang | 2012 | Hypertension2012，，3： | 1 |
| 9 | SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches显示文摘Estrogen receptor (ER)-positive luminal breast cancer is a subtype with generally lower risk of metastasis to most distant organs. However, bone recurrence occurs preferentially in luminal breast cancer. The mechanisms of this subtype-specific organotropism remain elusive. Here we show that an ER-regulated secretory protein SCUBE2 contributes to bone tropism of luminal breast cancer. Single-cell RNA sequencing analysis reveals osteoblastic enrichment by SCUBE2 in early bone-metastatic niches. SCUBE2 facilitates release of tumor membrane-anchored SHH to activate Hedgehog signaling in mesenchymal stem cells, thus promoting osteoblast differentiation. Osteoblasts deposit collagens to suppress NK cells via the inhibitory LAIR1 signaling and promote tumor colonization. SCUBE2 expression and secretion are associated with osteoblast differentiation and bone metastasis in human tumors. Targeting Hedgehog signaling with Sonidegib and targeting SCUBE2 with a neutralizing antibody both effectively suppress bone metastasis in multiple metastasis models. Overall, our findings provide a mechanistic explanation for bone preference in luminal breast cancer metastasis and new approaches for metastasis treatment. | Qiuyao Wu Pu Tian Dasa He Zhenchang Jia Yunfei He Wenqian Luo Xianzhe Lv Yuan Wang Peiyuan Zhang Yajun Liang Wenjin Zhao Jun Qin Peng Su Yi-Zhou Jiang Zhi-Ming Shao Qifeng Yang Guohong Hu | 2023 | Cell Research2023，33，6： | 1 |
| 10 | 显示文摘 | Ma Yunfei Zhang Jinlong Tian Baozhu | 2010 | Journal of Hazardous Materials2010，182，： | 1 |
| 11 | Aerosol composition,sources,and secondary processing during autumn at a regional site in the Beijing-Tianjin-Hebei region显示文摘Air pollution is serious during autumn in the Beijing-Tianjin-Hebei(BTH)region,but there are few studies that have utilized real-time observations and source apportionment of the autumn submicron aerosols in this region.In this study,a quadrupole aerosol chemical speciation monitor(Q-ACSM)was deployed for the real-time measurement of the non-refractory compositions of submicron aerosols(NR-PM1)at a regional site(Xianghe)from October 3 to November 14,2017.The results showed that nitrate was the largest inorganic aerosol,and the oxygenated organic aerosol(OOA)was the largest organic aerosol in Xianghe.Hydrocarbon-like OA(HOA)was the largest organic aerosol When the NR-PM1 mass concentrations increased from the lowest to the highest bins,nitrate and biomass burning OA(BBOA)showed increasing trends in the suburban area.Enhanced nitrate formation during the pollution epi-sodes resulted from both photochemical and aqueous processing.To reduce the particulate matter(PM2.5)concentrations and eliminate heavy pollution episodes,control measures should focus on reducing NO_(x),NH_(3),and volatile organic compound(VOCs)emissions. | Yichen Wang Qiyuan Wang Jie Tian Yong Zhang Yunfei Wu Weikang Ran Renjian Zhang Junji Cao | 2023 | Particuology2023，，4： | 0 |
| 12 | Estimation of PM2.5 Mass Concentration from Visibility显示文摘Aerosols in the atmosphere not only degrade visibility,but are also detrimental to human health and transportation.In order to develop a method to estimate PM2.5 mass concentration from the widely measured visibility,a field campaign was conducted in Southwest China in January 2019.Visibility,ambient relative humidity(RH),PM2.5 mass concentrations and scattering coefficients of dry particles were measured.During the campaign,two pollution episodes,i.e.,from 4-9 January and from 10-16 January,were encountered.Each of the two episodes could be divided into two periods.High aerosol hygroscopicity was found during the first period,when RH was higher than 80%at most of the time,and sometimes even approached 100%.The second period experienced a relatively dry but more polluted condition and aerosol hygroscopicity was lower than that during the first period.An empirical relationship between PM2.5 mass concentration and visibility(ambient aerosol extinction)under different RH conditions could thus be established.Based on the empirical relationship,PM2.5 mass concentration could be well estimated from visibility and RH.This method will be useful for remote sensing of PM2.5 mass concentration. | Denghui JI Zhaoze DENG Xiaoyu SUN Liang RAN Xiangao XIA Disong FU Zijue SONG Pucai WANG Yunfei WU Ping TIAN Mengyu HUANG | 2020 | Advances in Atmospheric Sciences2020，37，7： | 0 |
| 13 | A new method for evaluating the firing precision of multiple launch rocket system based on Bayesian theory显示文摘How to effectively evaluate the firing precision of weapon equipment at low cost is one of the core contents of improving the test level of weapon system.A new method to evaluate the firing precision of the MLRS considering the credibility of simulation system based on Bayesian theory is proposed in this paper.First of all,a comprehensive index system for the credibility of the simulation system of the firing precision of the MLRS is constructed combined with the group analytic hierarchy process.A modified method for determining the comprehensive weight of the index is established to improve the rationality of the index weight coefficients.The Bayesian posterior estimation formula of firing precision considering prior information is derived in the form of mixed prior distribution,and the rationality of prior information used in estimation model is discussed quantitatively.With the simulation tests,the different evaluation methods are compared to validate the effectiveness of the proposed method.Finally,the experimental results show that the effectiveness of estimation method for firing precision is improved by more than 25%. | Yunfei Miao Guoping Wang Wei Tian | 2024 | Defence Technology(防务技术)2024，32，2： | 0 |
| 14 | Salt-assisted vapor-liquid-solid growth of high-quality ultrathin nickel oxide flakes for artificial synapses in image recognition applications显示文摘Transition metal oxides have attracted intense interest owing to their abundant physical and chemical properties.The controlled preparation of large-area,high-quality two-dimensional crystals is essential for revealing their inherent properties and realizing high-performance devices.However,fabricating two-dimensional(2D)transition metal oxides using a general approach still presents substantial challenges.Herein,we successfully achieve highly crystalline nickel oxide(NiO)flakes with a thickness as thin as 3.3 nm through the salt-assisted vapor-liquid-solid(VLS)growth method,which demonstrated exceptional stability under ambient conditions.To explore the great potential of the NiO crystal in this work,an artificial synapse based on the NiO-flake resistive switching(RS)layer is investigated.Short-term and long-term synaptic behaviors are obtained with external stimuli.The artificial synaptic performance provides the foundation of the neuromorphic application,including handwriting number recognition based on artificial neuron network(ANN)and the virtually unsupervised learning capability based on generative adversarial network(GAN).This pioneering work not only paves new paths for the synthesis of 2D oxides in the future but also demonstrates the substantial potential of oxides in the field of neuromorphic computing. | Hui Zhang Zongjie Shen Alei Li Lin Wang Qinan Wang Yunfei Li Yunlei Zhong Juntong Zhu Yong Zhang Mengjiao Han Dan Tian Chun Zhao Lixing Kang Qingwen Li | 2024 | Nano Research2024，17，5： | 0 |
| 15 | Direct Radiative Effects of Dust Aerosols over Northwest China Revealed by Satellite-Derived Aerosol Three-Dimensional Distribution显示文摘Northwest China is recognized as a main source and a major transport channel of dust aerosols in East Asia.With a fragile ecological environment,this region is quite sensitive to global climate change.Based on the satellite-derived aerosol three-dimensional distribution,the direct radiative effects of dust aerosols over Northwest China are evaluated.Aerosols over Northwest China are mainly distributed in the Tarim Basin,Junggar Basin,Gobi Desert,and Loess Plateau.The aerosol extinction coefficients are greater than 0.36 km-1 over the Tarim Basin and 0.16 km^(-1) over the Gobi Desert and Loess Plateau,decreasing with height.Aerosols over Northwest China are mainly composed of pure dust and polluted dust.These dust aerosols can modify the horizontal temperature gradient,vertical thermodynamic structure,and diurnal temperature range by absorbing and scattering shortwave radiation and emitting longwave radiation.For the column atmosphere,the radiative effect of dust aerosols shows heating effect of approximately 0.3 K day^(-1) during the daytime and cooling effect of approximately-0.4 K day^(-1) at night.In the vertical direction,dust aerosols can heat up the lower atmosphere(0.5–1.5 K day^(-1))and cool down the upper atmosphere(about-1.0 K day^(-1))during the daytime,while they cool down the lower atmosphere(-3 to-1.5 K day^(-1))and heat up the upper atmosphere(1–1.5 K day^(-1))at night.There are also significant lateral and vertical variations in the dust radiative effects corresponding to their spatial distributions.This study provides some scientific basis for reducing uncertainty in the investigation of aerosol radiative effects and provides observation evidence for simulation studies. | Rui JIA Yuzhi LIU Yan LI Jun LI Xiaolin HU Ronglu GAO Yunfei TIAN Yanling SUN Nannan MU Minfen ZHAO | 2022 | Journal of Meteorological Research2022，36，5： | 0 |
| 16 | Mouse model for pangolin-origin coronavirus GX/P2V/2017 infection and cross-protection from COVID-19 ZF2001 subunit vaccine显示文摘Many severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)–related coronaviruses have been discovered,constituting potential threats to human health.However,it remains unclear whether the currently available vaccines are effective against these coronaviruses.Here,we constructed a wild-type mouse model to evaluate pathogenicity of the SARS-CoV-2-related pangolin coronavirus GX/P2V/2017 and neutralization efficacy of the approved tandemrepeat SARS-CoV-2 spike receptor-binding domain(RBD)vaccine ZF2001.We found that ZF2001-induced cross-reactive and cross-neutralizing antibodies against GX/P2V/2017,and the vaccination alleviated the pathological lung damage caused by GX/P2V/2017 in mice.These results indicate that RBD may work as a promising candidate for pan-coronavirus vaccine development. | Xiao Qu Yunfei Jia Na Jia Huahao Fan Anqi Zheng Luoyuan Xia Zhenfei Wang Di Tian Sheng Niu Yu Hu Wenxia Tian Zhihai Chen Yigang Tong Yuwei Gao Wuchun Cao Qihui Wang George Fu Gao | 2023 | hLife2023，1，1： | 0 |
| 17 | Characteristics of Gray Skin Chewing Sugarcane and Its Precise Cultivation Techniques显示文摘[Objectives] The aim was to provide technical supports for the popularization and application of gray skin chewing sugarcane in large areas in suitable regions. [Methods] Field planting tests were conducted in 2012-2016 to study the yield,major economic traits,disease resistances,growth periods,adaptability and cultivation techniques of gray skin chewing sugarcane. [Results]The characteristics and a set of precise cultivation techniques for gray skin chewing sugarcane were summarized. [Conclusions] The technology can be widely applied in the areas with altitude of less than 1 500 m and areas with similar climate in Yunnan Province. | Zhiming YANG Lin ZENG Xiao ZHAO Shunsheng LU Yunfei SONG Chaozhong ZHANG Wenwei TIAN | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
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