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| 1 | Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks显示文摘Magnolol rich in Magnolia officinalis is a bioactive polyphenolic compound. The aim of this study was to examine the effects of magnolol additive(MA) on growth performance, expression levels of antioxidantrelated genes, and intestinal mucosal morphology of Linwu ducks aged from 49 to 70 days, comparing with that of an antibiotic additive(colistin sulfate [CS]). A total of 275,49-day-old ducks were assigned to5 groups with 5 cages of 11 ducks each and fed diets supplemented with 0,100, 200 and 300 mg of MA/kg and 300 mg of CS/kg for 3 weeks, respectively. The results showed that the average daily body weight gain(ADG) was increased significantly in MA-fed groups(200 and 300 mg/kg), compared with the basal diet(BD) group(P < 0.05). The mRNA levels of superoxide dismutase-1(SOD1), manganese superoxide dismutase-2(MnSOD2) and catalase(CAT) were also increased significantly in MA groups(P < 0.05). In addition, hematoxylin and eosin staining revealed that Linwu ducks fed the diets with MA had more intact intestinal mucosa than those fed the BD and CS diets. In addition, ileal villus height, ileal villus height/crypt depth ratio(V/C) and duodenal V/C were also improved significantly(P < 0.05). Taken together, these data demonstrated that MA is an effective feed additive to enhance the growth performance of the Linwu ducks by improving the antioxidant and intestinal mucosal status, suggesting that MA will be a potential additive to replace antibiotic(CS). | Qian Lin Jianfei Zhao Kun Xie Yushi Wang Guili Hu Guitao Jiang Qiuzhong Dai Zhiyong Fan Jianhua He Xi He De-Xing Hou | 2017 | Animal Nutrition2017，，2： | 14 |
| 2 | Function identification of miR482b, a negative regulator during tomato resistance to Phytophthora infestans显示文摘Tomato is an important horticultural and economic crop cultivated worldwide.As Phytophthora infestans becomes a huge threat to tomato production,it is necessary to study the resistance mechanisms of tomato against P.infestans.Our previous research has found that miR482 might be involved in tomato–P.infestans interaction.In this study,miR482b precursor was cloned from Solanum pimpinellifolium“L3708”and miR482b was shown to decrease in abundance in tomato following P.infestans infection.Compared to wild-type tomato plants,tomato plants that overexpressed miR482b displayed more serious disease symptoms after P.infestans infection,with more necrotic cells,longer lesion diameters,and increased P.infestans abundance.Meanwhile,silencing of miR482b was performed by short tandem target mimic(STTM),resulting in enhancement of tomato resistance to P.infestans.Using miRNA and degradome data sets,NBS–LRR disease-resistance genes targeted by miR482b were validated.Negative correlation between the expression of miR482b and its target genes was found in all miR482b-overexpressing and-silencing tomato plants.Our results provide insight into tomato miR482b involved in the response to P.infestans infection,and demonstrate that miR482b–NBS–LRR is an important component in the network of tomato–P.infestans interaction. | Ning Jiang Jun Meng Jun Cui Guangxin Sun Yushi Luan | 2018 | Horticulture Research2018，5，1： | 13 |
| 3 | Tomato lncRNA23468 functions as a competing endogenous RNA to modulate NBS-LRR genes by decoying miR482b in the tomato-Phytophthora infestans interaction显示文摘Our previous studies indicated that tomato miR482b could negatively regulate the resistance of tomato to Phytophthora infestans and the expression of miR482b was decreased after inoculation with P.infestans.However,the mechanism by which the accumulation of miR482b is suppressed remains unclear.In this study,we wrote a program to identify 89 long noncoding RNA(lncRNA)-originated endogenous target mimics(eTMs)for 46 miRNAs from our RNA-Seq data.Three tomato lncRNAs,lncRNA23468,lncRNA01308 and lncRNA13262,contained conserved eTM sites for miR482b.When lncRNA23468 was overexpressed in tomato,miR482b expression was significantly decreased,and the expression of the target genes,NBS-LRRs,was significantly increased,resulting in enhanced resistance to P.infestans.Silencing lncRNA23468 in tomato led to the increased accumulation of miR482b and decreased accumulation of NBS-LRRs,as well as reduced resistance to P.infestans.In addition,the accumulation of both miR482b and NBS-LRRs was not significantly changed in tomato plants that overexpressed lncRNA23468 with a mutated eTM site.Based on the VIGS system,a target gene of miR482b,Solyc02g036270.2,was silenced.The disease symptoms of the VIGS-Solyc02g036270.2 tomato plants were in accordance with those of tomato plants in which lncRNA23468 was silenced after inoculation with P.infestans.More severe disease symptoms were found in the modified plants than in the control plants.Our results demonstrate that lncRNAs functioning as eTMs may modulate the effects of miRNAs in tomato and provide insight into how the lncRNA23468-miR482b-NBS-LRR module regulates tomato resistance to P.infestans. | Ning Jiang Jun Cui Yunsheng Shi Guanglei Yang Xiaoxu Zhou Xinxin Hou Jun Meng Yushi Luan | 2019 | Horticulture Research2019，6，1： | 12 |
| 4 | A novel Co(phen)_2/C catalyst for the oxygen electrode in rechargeable lithium air batteries显示文摘A novel Co(phen)2/C catalyst was prepared by coating cobalt(II) phenanthroline (phen) chelate on BP2000 carbon black and then heat treating in an inert atmosphere. The obtained Co(phen)2/C product with 1.0 wt% cobalt loading exhibits similar morphology and porosity characteristics to those of the bare BP2000. X-ray diffraction measurements demonstrate a face-centered cubic (fcc) α-Co phase embedded in the carbon support after pyrolysis. Charge/discharge tests of the lithium-oxygen cells using the prepared Co(phen)2/C catalyst show high discharge capacities of 4870 mAh g-1 (0.05 mA cm-2 ), 3353 mAh g-1 (0.1 mA cm-2 ) and 3220 mAh g 1 (0.15 mA cm-2 ), respectively. The Co(phen) 2 /C cathode exhibits reasonable reversibility with capacity retention of 1401 mAh g-1 ( 0.1 mA cm-2 ) after 10 cycles. The superior electrochemical performance of the prepared Co(phen)2/C catalyst and low cost of the phenanthroline chelating agent indicate that Co(phen)2/C is a promising cheap catalyst for lithium-air batteries. | WANG Hong LIAO XiaoZhen JIANG QiZhong YANG XiaoWei HE YuShi Ma ZiFeng | 2012 | Chinese Science Bulletin2012，57，16： | 4 |
| 5 | Study ofphysiological parameters and comfort sensations duringfriction contacts of human skin显示文摘 | LI Wei PANG Qiang JIANG Yushi | 2012 | Tribology Letters2012，48，3： | 1 |
| 6 | Formation Mechanism and Occurrence Law of Pod Shattering in Soybean:A Review显示文摘Seed shattering refers to the phenomenon in which the pods split along the abdominal and back sutures before the crop is received,so that the seeds are spread.Seed shattering is vital to the reproduction of their offspring in wild plants,but it is also the main cause of crop yield loss reason.Pod-explosion resistance is a complex process of physical and physiological and biochemical reactions.Soybean seed shattering phenomenon is widespread,which severely restricts the development of soybean industry.Seed shattering(pod cracking or fruit dropping)is essential for the reproduction of its offspring in wild plants,but it is also the main cause of crop yield loss.This article analyzes the morphology and structure of pods related to seed shattering from the morphology of pods.On the basis of the regularity of the occurrence of seed shattering and the summary of phenotypic index identification methods,physiologically introduced the regulation mechanism of key enzymes and endogenous hormones on seed shattering.The localization,labeling and cloning of seed shattering genes are introduced in molecular biology.The study focused on reviewing the latest advances in the research on soybean seed shattering characteristics,and discussed with the research results of related crops.Finally,the research and application of soybean seed shattering resistance were prospected for several aspects. | Jinfeng Liu Yuzhe Zhang Yushi Jiang Hongji Sun Ruijie Duan Jing Qu Dan Yao Siyan Liu Shuyan Guan | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 1 |
| 7 | Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance显示文摘Exploring the molecular mechanism of soybean response to drought stress,providing a basis for genetic improvement and breeding of heat-resistant varieties,relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18)and Jinong 18 mutant(JB18)soybeans,using reverse transcription,one gene in the sHSP family was cloned using PCR(RT-PCR)and it was named sHSP26.In this experiment,the soybean sHSP26 gene was successfully cloned by RT-PCR,the protein encoded by the sHSP26 gene was analyzed by bioinformatics,and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were constructed.The positive plants were derived from Agrobacterium-mediated transformation of soybean cotyledon nodes,and T2 plants were identified through conventional PCR,QT-PCR,and Southern blot hybridization.Finally,through the determination of drought-related physiological and biochemical indicators and the analysis of agronomic traits,further research on gene function was conducted.The results indicated that the overexpression vector plant GmsHSP26 gene expression increased.After stress,the SOD and POD activities,and the PRO content of the transgenic overexpression plants increased,while the MDA content decreased.The reverse was true for soybean plants with genetically modified editing vectors.A survey of agronomic traits indicated that the fourpod ratio and yield per plant of the transgenic overexpression plants were higher than those of the control and transgenic editing vector soybean plants.It indicates that the expression of the sHSP26 gene can enhance drought resistance and soybean yield.The soybean sHSP26 gene cloning and its functional verification have not yet been reported.This is the first report where PCR amplification of soybean sHSP26 genes and gene expression vector were applied.It lays the foundation for creating new drought-resistant transgenic soybean lines through genetic engineering technology and is essential for improving soybean yield and quality. | Siyan Liu Jinfeng Liu Yuzhe Zhang Yushi Jiang Shaowang Hu Andi Shi Qiyao Cong Shuyan Guan Jing Qu Yao Dan | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 1 |
| 8 | The effects of public spending of Pension insurance and human capital显示文摘 | Yu Lingyun Jiang yushi | | 0，，02： | 1 |
| 9 | A new model for Doppler shift of C-band echoes backscattered from sea surface显示文摘Within the framework of the two-scale scattering model,the Doppler shift of C-band radar return signals from the nonlinear sea surface are numerically evaluated.As an analytical approximation method,the Bragg resonance scattering method cannot accurately describe the backscattering field from sea surface.Therefore,in the twoscale scattering model,more accurate scattering coefficient(the normalized radar cross section,NRCS)evaluated by the C-band dual-polarized(HH/VV)empirical geophysical model function(CSAR model)is employed to replace the traditional Bragg NRCS to weight the Doppler shift.The numerical results indicate that there are obvious differences between the Doppler shift weighted by the CSAR NRCS and that weighted by the traditional Bragg NRCS.The hydrodynamic modulation of the large-scale waves is one of the important factors that affect the difference between the Doppler shift predicted in upwind and downwind directions.If the relaxation rate in the hydrodynamic modulation is set to be the angular frequency of the dominant water waves,the Doppler shift predicted by the numerical method can fit the results of the empirical model(C-band empirical geophysical model function,CDOP)well at moderate wind speed.Under low wind condition,the comparison shows that the empirical CDOP model appears to overestimate the Doppler shift.In order to facilitate the application,at the end of this paper a semi-empirical CSAR-DOP model,which is a polynomial fitting formula,is developed for evaluating the Doppler shift of C-band signals from time varying sea surface. | Jianbo Cui Yunhua Wang Yanmin Zhang Huimin Li Wenzheng Jiang Yushi Zhang Xin Li | 2023 | Acta Oceanologica Sinica2023，42，6： | 0 |
| 10 | Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene显示文摘Soybean(Glycine max(L.)Merr.)is an important cultivated crop,which requires much water during its growth,and drought seriously affects soybean yields.Studies have shown that the expression of small heat shock proteins can enhance drought resistance,cold resistance and salt resistance of plants.In this experiment,soybean GmHsps_p23-like gene was successfully cloned by RT-PCR,the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis,and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed.Agrobacterium-mediated method was used to transform soybeans to obtain positive plants.RT-PCR detection,rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T2 generation positive transgenic soybean plants identified by PCR and Southern hybridization.The results showed that the overexpression vector plant GmHsps_p23-like gene expression increased.After rehydration,the transgenic overexpression plants returned to normal growth,and the damage to the plants was low.After drought stress,the SOD and POD activities and the PRO content of the transgenic overexpression plants increased,while the MDA content decreased.The reverse was true for soybean plants with genetically modified editing vectors.The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group,and had a stronger drought resistance.It showed that the expression of soybean GmHsps_p23-like gene can improve the drought resistance of soybean.The cloning and functional verification of soybean GmHsps_p23-like gene had not been reported yet.This is the first time that PCR technology has been used to amplify the soybean GmHsps_p23-like gene and construct an expression vector for this gene.This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties. | Yuzhe Zhang Guanglong Li Shaowang Hu Jinfeng Liu Yushi Jiang Siyan Liu Shuyan Guan Jing Qu Dan Yao Andi Shi Yixuan Liu | 2022 | Phyton-International Journal of Experimental Botany2022，91，6： | 0 |
| 11 | Identification of microbial metabolites that accelerate the ubiquitindependent degradation of c-Myc显示文摘Myc belongs to a family of proto-oncogenes that encode transcription factors.The overexpression of c-Myc causes many types of cancers.Recently,we established a system for screening c-Myc inhibitors and identified antimycin A by screening the RIKEN NPDepo chemical library.The specific mechanism of promoting tumor cell metastasis by high c-Myc expression remains to be explained.In this study,we screened approximately 5,600 microbial extracts using this system and identified a broth prepared from Streptomyces sp.RK19-A0402 strongly inhibits c-Myc transcriptional activity.After purification of the hit broth,we identified compounds closely related to the aglycone of cytovaricin and had a structure similar to that of oligomycin A.Similar to oligomycin A,the hit compounds inhibited mitochondrial complex V.The mitochondria dysfunction caused by the compounds induced the production of reactive oxygen species(ROS),and the ROS activated GSK3α/βthat phosphorylated c-Myc for ubiquitination.This study provides a successful screening strategy for identifying natural products as potential c-Myc inhibitors as potential anticancer agents. | ZIYU LIU AKIKO OKANO EMIKO SANADA YUSHI FUTAMURA TOSHIHIKO NOGAWA KOSUKE ISHIKAWA KENTARO SEMBA JIANG LI XIAOMENG LI HIROYUKI OSADA NOBUMOTO WATANABE | 2023 | Oncology Research2023，31，5： | 0 |
| 12 | An analysis of the uniformity of multi-fracture initiation based on downhole video imaging technology: A case study of Mahu tight conglomerate in Junggar Basin, NW China显示文摘To solve the problem that the production of Mahu conglomerate reservoir is not up to expectation after the multi-cluster plus temporary plugging fracturing technology is applied in horizontal wells, stages 2–6 in the test well MaHW6285 are selected to carry out erosion tests with different pumping parameters. The downhole video imaging technology is used to monitor the degree of perforations erosion, and then the fracture initiation and proppant distribution of each cluster are analyzed. The results showed that proppant entered 76.7% of the perforations. The proppant was mainly distributed in a few perforation clusters, and the amount of proppant entered in most of the clusters was limited. The proppant distribution in Stage 4 was relatively uniform, and the fracture initiation of each cluster in the stage is more uniform. The proppant distribution in stages 2, 3, 5, and 6 was significantly uneven, and the uniform degree of fracture initiation in each cluster is low. More than 70% of the proppant dose in the stage entered clusters near the heel end, so the addition of diverters did not promote the uniform initiation of hydraulic fractures. There was a positive correlation between the amount of proppant added and the degree of perforations erosion, and the degree of perforations erosion ranged from 15% to 352%, with an average value of 74.5%, which was far higher than the statistical results of shale reservoir tests in North America. The use of 180° phase perforation(horizontal direction) can reduce the “Phase Bias” of perforations erosion, promote uniform perforations erosion and fluid inflow. The research results provide the basis for optimizing the pumping procedure, reducing the perforation erosion and improving the success rate of diversion. | ZANG Chuanzhen JIANG Hanqiao SHI Shanzhi LI Jianmin ZOU Yushi ZHANG Shicheng TIAN Gang YANG Peng | 2022 | Petroleum Exploration and Development2022，49，2： | 0 |
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