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| 1 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 2 | Identification and differentiation of major components in three different “Sheng-ma” crude drug species by UPLC/Q-TOF-MS显示文摘Cimicifugae Rhizoma(Sheng ma) is a Ranunculaceae herb belonging to a composite family and well known in China,has been widely used in traditional Chinese medicine.The Pharmacopoeia of the People's Republic of China contains three varieties(Cimicifuga dahurica(Turcz.),Cimicifuga foetida L.and Cimicifuga heracleifolia Kom.) which have been used clinically as 'Sheng-ma'.However,the chemical constituents of three components of 'Sheng-ma' have never been documented.In this study,a rapid method for the analysis of the main components of 'Sheng-ma' was developed using ultra-high performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC/Q-TOF-MS).The present study reveals the major common and distinct chemical constituents of C.dahurica,C.foetida and C.heracleifolia and also reports principal component and statistical analyses of these results.The components were identified by comparing the retention time,accurate mass,mass spectrometric fragmentation characteristic ions and matching empirical molecular formula with that of the published compounds.A total of 32 common components and 8 markers for different 'Sheng-ma' components were identified.These findings provide an important basis for the further study and clinical utilities of the three 'Sheng-ma' varieties. | Mengxue Fan Kunming Qin Fei Ding Yuting Huang Xiaoli Wang Baochang Cai | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 4 |
| 3 | INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE显示文摘Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases.The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations.Interactions between fennel(Foeniculum vulgare)roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco(Nicotiana tabacum).The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P.nicotianae were assessed.The roots of fennel attracted P.nicotianae zoospores and inhibited their motility and the germination of cystospores,with some cystospores rupturing.4-ethylacetophenone,vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P.nicotianae,especially vanillin.Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species.These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P.nicotianae. | Yuxin YANG He ZHANG Yuting FANG Ying LI Xinyue MEI Huichuan HUANG Fei DU Shusheng ZHU Min YANG Yixiang LIU | 2021 | Frontiers of Agricultural Science and Engineering2021，8，3： | 3 |
| 4 | Generation of purple-violet chrysanthemums via anthocyanin B-ring hydroxylation and glucosylation introduced from Osteospermum hybrid F3'5'H and Clitoria ternatea A3'5'GT显示文摘Chrysanthemums possess no metabolic pathway to synthesize delphinidin because of the lack of endogenous F3'5'H gene encoding the key enzyme in its biosynthetic pathway;therefore,there are no blue or blue-purple chrysanthemums occurring naturally.Currently,the introduction of exogenous F3'5'H into chrysanthemums is an efficient method for breeding bluish chrysanthemums.In this study,we explored the effects of the introduction of mutant CmF3'H(generated via site-directed mutagenesis,T485S,CmF3'Hm)and exogenous Osteospermum hybrid F3'5'H(OhF3'5'H)genes combined with Clitoria ternatea A3'5'GT(CtA3'5'GT)on delphinidin synthesis in chrysanthemum.Among the F3'5'H transgenic lines,those overexpressing endogenous CmF3'Hm could not generate blue flower color,although red color was changed to light pink due to CtA3'5'GT function.Meanwhile,OhF3'5'H introduction promoted the accumulation of delphinidin and its derivatives in chrysanthemum,changing the flower color from red-purple to purple-violet.These results indicate the applicability of exogenous OhF3'5'H and CtA3'5'GT transformation for promoting delphinidin synthesis during the molecular breeding of violet/blue chrysanthemums. | Xiaoying Han Yuting Luo Jiaoyang Lin Huiying Wu Hao Sun Lijie Zhou Sumei Chen Zhiyong Guan Weimin Fang Fei Zhang Fadi Chen Jiafu Jiang | 2021 | Ornamental Plant Research2021，1，1： | 3 |
| 5 | HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway显示文摘As a group of nonspecific inflammatory diseases affecting the intestine,inflammatory bowel disease(IBD)exhibits the characteristics of chronic recurring inflammation,and was proven to be increasing in incidence(Kaplan,2015).IBD induced by genetic background,environmental changes,immune functions,microbial composition,and toxin exposures(Sasson et al.,2021)primarily includes ulcerative colitis(UC)and Crohn's disease(CD)with complicated clinical symptoms featured by abdominal pain,diarrhea,and even blood in stools(Fan et al.,2021;Huang et al.,2021). | Yuting XU Li ZHANG Dickson Kofi Wiredu OCANSEY Bo WANG Yilin HOU Rong MEI Yongmin YAN Xu ZHANG Zhaoyang ZHANG Fei MAO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，5： | 1 |
| 6 | Cinnamon and nisin in alginate–calcium coating maintain quality of fresh northern snakehead fish fillets显示文摘 | Fei Lu Yuting Ding Xingqian Ye Donghong Liu | 2010 | LWT - Food Science and Technology2010，，9： | 1 |
| 7 | Analysis of a Cold Wave Process in Jiujiang and Its Numerical Model Forecast显示文摘The cold wave weather process in Jiujiang in the early spring of February 2020 was analyzed.The results show that the establishment of blocking high near Lake Baikal and the rapid southward of cold air after accumulation resulted in the cold wave weather accompanied by strong cooling,hale and rain(snow)weather in Jiujiang.Before the cold wave broke out,the ground warmed up significantly,which was also one of thermal conditions for this cold wave weather.Water vapor conditions were abundant at middle and low levels;at 850 hPa,temperature dropped by 12-14℃during February 14-15,and-4℃isotherm appeared in the southern part of central Jiangxi,which is a favorable condition for rain(snow)in most areas of Jiujiang. | Jingjing ZHANG Yuting FEI Rong LI | 2021 | Meteorological and Environmental Research2021，12，3： | 1 |
| 8 | Climate technology transfer in BRI era:needs,priorities,and barriers from receivers’perspective显示文摘Technology is an instrument to build BRI relationships,mitigate the environmental and climate impacts of BRI projects,as well as to enhance environmental sustainability in the regions.This study aims to reposition China in global climate technology transfer in BRI era and to obtain initial knowledge on needs,priorities,and barriers from the receivers’perspective.Focus group method with aid of questionnaire survey and follow-up face-to-face interviews was adopted to capture the major issues directly expressed by receivers from these countries.A total of 63 valid questionnaires were collected,and 13 respondents were face-to-face interviewed.The results confirmed that energy and agriculture were the most prioritized sectors for mitigation and adaptation in the developing countries alongside OBOR.The prioritized technologies for mitigation included cogeneration,solar photovoltaic,and biomass/biogas electricity.Irrigation,conservation agriculture,and soil management were prioritized for adaptation in agricultural sector,and water recycling and reuse,source water protection,and urban drainage management in water resource sector.Technology cost during installation and operation was stressed as the most important factor constraining the application and diffusion of climate technologies.But communication including language,information,and ways of communication,was also identified as an important factor.This implied that the conventional climate technology transfer need adapt to changing contexts of BRI and be complemented with innovative approaches involving multi-actors in different phases of climate technology development.Due to the limited representativeness of the sample,the results can hardly be generalized to all the countries,but raised interesting topics for future researches. | Minpeng Chen Lei Zhang Fei Teng Jingjing Dai Zhuang Li Ziqi Wang Yuting Li | 2020 | Ecosystem Health and Sustainability2020，6，1： | 1 |
| 9 | Assessment of heavy metal pollution in Laizhou Bay(China)using the ecological risk index and the integrated biomarker response of the goby Acanthogobius ommaturus显示文摘We used the Integrated Biological Responses version 2(IBRv2)method to evaluate the biological eff ects of heavy metals in the sediments in Laizhou Bay,China on the benthic goby Acanthogobius ommaturus.In December 2018,gobies and sediments were collected from 15 stations.We measured the activities of defense enzymes and the contents of malondialdehyde(MDA)and metallothionein(MT)in the goby liver as well as the levels of heavy metals in the sediments and goby muscle tissue.Most of the heavy metal concentrations in sediment at each station were below the Class I criteria set by Chinese Standards for Marine Sediment Quality,and the Håkanson ecological risk index suggested low risk for the heavy metals.We found that A.ommaturus could eff ectively accumulate mercury,cadmium,arsenic,and zinc and that the contents of MT and MDA and the activities of glutathione peroxidase and glutathione reductase were suitable biomarkers of heavy metal pollution in this species.The IBRv2 method integrated these four biomarkers and discriminated stations according to heavy metal pollution.Higher IBRv2 values suggested more adverse eff ects in gobies,corroborating more serious heavy metal contamination.The stations with high IBRv2 values and high contents of heavy metals were mainly distributed in the west and northeast parts of the bay.These results show that the IBRv2 approach is a feasible strategy for assessing heavy metal pollution through biological response and biological status and that it can be implemented for environmental monitoring in Laizhou Bay. | Maosheng LIU Hua XU Qiang ZOU Fei FANG Shan SUN Yuting ZHAO Xin HE Yonghui BO Lei YAO Yan FANG | 2023 | Journal of Oceanology and Limnology2023，41，4： | 0 |
| 10 | Study on the Performance of Organic Rankine Cycle-Heat Pump(ORC-HP) Combined System Powered by Diesel Engine Exhaust显示文摘This study presents an ORC-HP combined system driven by diesel engine exhaust. This paper focuses on the feasibility and performance of the combined system under heating mode to heat a coach. The performances of the combined system with different parameters, including condensation temperature and evaporation pressure in ORC system, gas cooler outlet pressure, gas cooler outlet temperature and evaporation temperature in HP system, have been analyzed. The results show that the combined system can fully meet the demand of heat production. The optimal flow division ratio(rp) of 0.32 is selected by analyzing the system performance. Low condensation temperature is beneficial to the combined system performance. There exists a suitable range of evaporation temperature, gas cooler outlet pressure and gas cooler outlet temperature to achieve excellent performance. The heating coefficient of performance(COP_h) shows a first increasing and then decreasing trend with gas cooler outlet pressure, namely there exists an optimal gas cooler outlet pressure that achieves the maximum COP_h. The maximum total efficiency, COP_h and heating capacity can reach up to 48.44%, 4.78 and 176.56 kW, respectively. These results show significant energy savings by applying the ORC-HP combined system. | ZHAO Tenglong YU Fei ZHANG Hongguang WU Yuting WANG Yan | 2019 | Journal of Thermal Science2019，28，5： | 0 |
| 11 | A novel synthesized prodrug of gemcitabine based on oxygen-free radical sensitivity inhibited the growth of lung cancer cells显示文摘In the present study,we introduced the H2O2-sensitive thiazolidinone moiety at the 4th amino group of gemcitabine(GEM)to synthesize a new target compound named GEM-ZZQ,and then we confirmed its chemical structure by nuclear magnetic resonance spectroscopy.We further confirmed that GEM-ZZQ had a good chemical stability in different pH solutions in vitro and that it could be activated by H2O2 to release GEM.Pharmacodynamic studies revealed that the growth inhibition of human normal epithelial cells was weaker by GEM-ZZQ than by GEM treatment and that the inhibition of various lung cancer cell lines by GEM-ZZQ was similar to that of GEM.For the lung cancer cell lines that are resistant to the epidermal growth factor receptor(EGFR)-targeting inhibitor osimertinib,GEM-ZZQ showed less growth inhibition than GEM;however,GEM-ZZQ in combination with cisplatin showed better synergistic effects than GEM in the low-dose groups.In summary,we provided a new anti-cancer compound GEM-ZZQ for treating lung cancer by modifying the GEM structure. | Xinlu Chai Yuting Meng Wei Ge Juan Wang Fei Li Xue Jun Wang Xuerong Wang | 2023 | The Journal of Biomedical Research2023，37，5： | 0 |
| 12 | miRNA-195:The New Target of Inhibiting the Proliferation,Migration and Invasion of Gastric Cancer Cells via Propofol显示文摘Objective:To explore the effect of propofol(Prof)on the proliferation,migration and invasion of human gastric cancer cell MGC-803 and its molecular mechanism.Methods:The MTT method was used to study the effects of Prof with different doses and durations on the viability of MGC-803 cells.Hoechst 33258 staining and electron microscopy were used to detect the effects of Prof on MGC-803 cell apoptosis.Transwell experiments were used to detect the effects of Prof on the migration and invasion of MGC-803 cells.RT-PCR detects the effect of Prof on the expression of miR-195 in MGC-803 cells,and Western Blot detects the effect of Prof on the protein expression of JAK/STAT signaling pathway.Results:Compared with 0μg/ml Prof,5μg/ml,10μg/ml and 20μg/ml Prof treatment with 24h,48h and 72h can significantly reduce cell viability(P<0.05).Compared with the Control group,the percentage of Hoechst 33258 staining positive cells in the Prof group and the apoptosis rate under the electron microscope were significantly increased(P<0.05).Compared with the Control group,the cell migration rate and invasion rate of the Prof group were significantly reduced(P<0.05).Compared with the Control group,the expression of miRNA-195 in the Prof group cells was increased significantly(P<0.05).Compared with the Control group,the activity of p-Jak1 and p-STAT3 proteins in the Prof group were significantly reduced(P<0.05).Conclusion:Prof can reduce the cell viability,migration and invasion of gastric cancer cell MGC-803,and promote its apoptosis.Its mechanism may be related to the promotion of miR-195 expression and inhibition of JAK/STAT signal pathway activity. | Yuming Zhang Zhijun Mao Fei Xue Yu Zhang Yuting Min Jun Wang | 2021 | Proceedings of Anticancer Research2021，5，2： | 0 |
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