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| 1 | Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus显示文摘AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells. | Shuang Ping Guo~1 Wen Liang Wang~1 Yu Qiang Zhai~2 Yi Ling Zhao~1 ~1Department of Pathology,Xijing Hospital of the Fourth Military Medical University,Xi’an,China ~2Department of Urology,the Central Hospital of Xi’an,China | 2001 | World Journal of Gastroenterology2001，7，3： | 55 |
| 2 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 3 | Changes in Waist Circumference and Abdominal Obesity among Chinese Adults over a Ten-year Period显示文摘Objective The aim of this study was to describe changes in waist circumference(WC) and prevalence of abdominal obesity over a period of 10 years among Chinese adults in different socio-economic status(SES). Methods Data derived from the China Nutrition and Health Surveillance during 2002 and 2010-2012. We calculated the mean WC and the prevalence of abdominal obesity by gender, place of residence, SES indicators(education, income, and marital status), and body mass index(BMI) categoriesand used pooled t-tests to assess the differences between the two time periods. Results 26.0% of men and 25.3% of women had abdominal obesity in 2010-2012. The age-adjusted mean WC increased by 2.7 cm among men and 2.1 cm among women; the age-adjusted prevalence of abdominal obesity increased by 7.7% among men and 5.3% among women. The rising trends were observed in all subgroups except for a negative growth in high-income women. People living in rural areas with low education and income and with a BMI of 18.5 to 23.9 kg/m^2 had a greater absolute and relative increase in WC. People living in rural areas with low income had a greater relative increase in abdominal obesity. Conclusion The mean WC and prevalence of abdominal obesity among Chinese adults have increased during the past 10 years. Gender differences were noted using various SES indicators. | ZHAI Yi FANG Hong Yun YU Wen Tao YU Dong Mei ZHAO Li Yun LIANG Xiao Feng ZHAO Wen Hua | 2017 | Biomedical and Environmental Sciences2017，30，5： | 19 |
| 4 | Genome Characteristics and Evolution of Pseudorabies Virus Strains in Eastern China from 2017 to 2019显示文摘Since late 2011,outbreaks of pseudorabies virus(PRV)have occurred in southern China causing major economic losses to the pig industry.We previously reported that variant PRV forms and recombination in China could be the source of continued epidemics.Here,we analyzed samples from intensive pig farms in eastern China between 2017 and 2019,and sequenced the main glycoproteins(gB,gC,gD,and gE)to study the evolution characteristics of PRV.Based on the gC gene,we found that PRV variants belong to clade 2 and detected a founder effect during by the PRV epidemic.In addition,we detected inter-and intra-clade recombination;in particular,inter-clade recombination in the gB genes of strains FJ-ZXF and FJ-W2,which were recombinant with clade 1 strains.We also found specific amino-acid changes and positively selected sites,possibly associated with functional changes.This analysis of the emergence of PRV in China illustrates the need for continuous monitoring and the development of vaccines against specific variants of PRV. | Xiaofeng Zhai Wen Zhao Kemang Li Cheng Zhang Congcong Wang Shuo Su Jiyong Zhou Jing Lei Gang Xing Haifeng Sun Zhiyu Shi Jinyan Gu | 2019 | Virologica Sinica2019，34，6： | 12 |
| 5 | Numerical study on Rayleigh-Taylor effect on cylindrically converging Richtmyer-Meshkov instability显示文摘Evolution of a two-dimensional air/SF6 single-mode interface is numerically investigated by an upwind CE/SE method under a cylindrically converging circumstance. The Rayleigh-Taylor effect caused by the flow deceleration on the phase inversion(RTPI)is highlighted. The RTPI was firstly observed in our previous experiment, but the related mechanism remains unclear. By isolating the three-dimensional effect, it is found here that the initial amplitude(a0), the azimuthal mode number(k0) and the re-shocking moment are the three major parameters which determine the RTPI occurrence. In the variable space of(k0, a0), a critical a0 for the RTPI occurrence is solved for each k0, and there exists a threshold value of k0 below which the RTPI will not occur no matter what a0 is. There exists a special k0 corresponding to the largest critical a0, and the reduction rule of critical a0 with k0 can be well described by an exponential decay function. The results show that the occurrence of the RTPI requires a small a0 which should be less than a critical value, a large k0 which should exceed a threshold, and a right impinging moment of the re-shock which should be later than the RTPI occurrence. Finally, the effects of the incident shock strength, the density ratio and the initial position of the interface on the threshold value of k0 and on the maximum critical a0 are examined. These new findings would facilitate the understanding of the converging Richtmyer-Meshkov instability and would be helpful for designing an optimal structure of the inertia confinement fusion capsule. | ZhiGang Zhai Fu Zhang ZhangBo Zhou JuChun Ding Chih-Yung Wen | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，12： | 9 |
| 6 | Estimated cancer incidence and mortality in Hebei province,2012显示文摘Objective: This study estimates the numbers of new cancer cases and cancer deaths in Hebei province using incidence and mortality data from 9 population-based cancer registries in 2012.Methods: The data of new diagnosed cancer cases and cancer deaths in 2012 were collected from 9 population-based cancer registries of Hebei province in 2015. All the data met the National Central Cancer Registry of China(NCCR) criteria of data quality. The pooled data analysis was stratified by areas(urban/rural), gender, age group(0, 1-4, 5-9, 10-14, …, 85+) and cancer type. New cancer cases and deaths in Hebei province were estimated using age-specific rates and corresponding provincial population in 2012. The 10 most common cancers in different groups and the cumulative rates were calculated. Chinese population census in 2000 and Segi's population were used for age-standardized incidence/mortality rates. Results: All cancer registries covered 4,986,847 populations, 6.84% of Hebei provincial population(2,098,547 in urban and 2,888,300 in rural areas). The percentage of cases morphologically verified(MV%) and death certificate-only cases(DCO%) were 76.40% and 4.72%, respectively. The mortality to incidence rate ratio(M/I) was 0.64. In 2012, it is estimated that there were about 187,900 new diagnosed cancer cases and 119,800 cancer deaths in Hebei province. The incidence rate of cancer was 258.12/100,000(275.75/100,000 in males, 239.78/100,000 in females), and the age-standardized incidence rates by Chinese standard population(ASIRC) and by world standard population(ASIRW) were 210.65/100,000 and 208.50/100,000, with the cumulative incidence rates(0-74 years old) of 24.46%. The cancer incidence and ASIRC were 256.99/100,000 and 211.32/100,000 in urban areas and 258.94/100,000 and 209.99/100,000 in rural areas, respectively. The cancer mortality rate was 164.63/100,000(201.85/100,000 in males, 125.92/100,000 in females). Agestandardized mortality rates by Chinese standard population(ASMRC) and by world standard population(ASMRW) were 137.30/100,000 and 137.39/100,000 with the cumulative mortality rate(0-74 years old) of 14.58%, respectively. The cancer mortality rate in rural areas(167.16/100,000) was higher than that in urban areas(161.16/100,000). The most common cancers were lung cancer, stomach cancer, breast cancer, esophageal cancer, liver cancer and colorectal cancer, which accounted for 72.31% of all cancer cases. Lung cancer, stomach cancer, liver cancer, esophageal cancer and colorectal cancer were the major causes of cancer death in Hebei province, which accounted for 75.24% of all cancer deaths. The cancer spectrum differs between urban and rural, males and females in both incidence and mortality rates.Conclusions: The most common cancers were lung cancer, stomach cancer, esophageal cancer, breast cancer, liver cancer and colorectal cancer in Hebei province. | Yutong He Di Liang Daojuan Li Jingbo Zhai Junqing Zhu Jing Jin Denggui Wen Baoen Shan | 2016 | Chinese Journal of Cancer Research2016，28，3： | 8 |
| 7 | Molecular evolution of the rice miR395 gene family显示文摘MicroRNAs (miRNAs) are 20-22 nucleotide non-coding RNAs that play important roles in plant and animal development. They are usually processed from larger precursors that can form stem-loop structures. Among 20 miRNA families that are conserved between Arabidopsis and rice, the rice miR395 gene family was unique because it was organized into compact clusters that could be transcribed as one single transcript. We show here that in fact this family had four clusters of total 24 genes. Three of these clusters were segmental duplications. They contained miR395 genes of both 120 bp and 66 bp long. However, only the latter was repeatedly duplicated. The fourth cluster contained miR395 genes of two different sizes that could be the consequences of intergenic recombination of genes from the first three clusters. On each cluster, both 1-duplication and 2-duplication histories were observed based on the sequence similarity between miR395 genes, some of which were nearly identical suggesting a recent origin. This was supported by a miR395 locus survey among several species of the genus Oryza, where two clusters were only found in species with an AA genome, the genome of the cultivated rice. A comparative study of the genomic organization of Medicago truncatula miR395 gene family showed significant expansion of intergenic spaces indicating that the originally clustered genes were drifting away from each other. The diverse genomic organizations of a conserved microRNA gene family in different plant genomes indicated that this important negative gene regulation system has undergone dramatic tune-ups in plant genomes. | Sreelatha GUDDETI De Chun ZHANG Ai Li LI Chuck H. LESEBERG Hui KANG Xiao Guang LI Wen Xue ZHAI Mitrick A. JOHNS Long MAO | 2005 | Cell Research2005，15，8： | 7 |
| 8 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 9 | N-Soliton Solutions of General Nonlinear Schrdinger Equation with Derivative显示文摘有衍生物(GDNLSE ) 和 N-soliton 答案的一般非线性的 Schrödinger 方程的双线性的方程分别地通过依赖可变转变和 Hirota 方法被获得。有衍生物(DNLSE ) 和它的 multisoliton 答案的非线性的 Schrödinger 方程的双线性的方程被减小给。 | ZHAI Wen CHEN Deng-Yuan | 2008 | Communications in Theoretical Physics2008，49，5： | 6 |
| 10 | Plasmonically induced transparency in double-layered graphene nanoribbons显示文摘Near-field coupled plasmonic systems generally achieve plasmonically induced transparency(PIT) using only one-way bright–dark mode coupling. However, it is challenging to realize such well-designed devices,mainly because they depend significantly on the polarization direction. We exploit surface plasmons supported by two crossed layers of graphene nanoribbons(GNRs) to achieve dynamically tunable PIT, where each GNR operates as both the bright and dark modes simultaneously. The proposed PIT can result from either one-way bright–dark mode interactions or bidirectional bright–bright and bright–dark mode hybridized coupling when the polarization is perpendicular/parallel or at an angle to the GNRs, respectively.Additionally, identical ribbon widths yield polarization-insensitive single-window PIT, whereas different ribbon widths produce polarization-dependent double-window PIT. We examine the proposed technique using plasmon wave functions and the transfer matrix method; analytical and numerical results show excellent agreement. This study can provide physical insight into the PIT coupling mechanisms and advance the applicability and versatility of PIT-based sensing platforms and other active devices. | SHENG-XUAN XIA XIANG ZHAI LING-LING WANG SHUANG-CHUN WEN | 2018 | Photonics Research2018，6，7： | 6 |
| 11 | Validity and Reliability of Chinese Physical Activity Questionnaire for Children Aged 10-17 Years显示文摘Objective This study is aimed to report the development,the reliability and validity of the Chinese Children Physical Activity Questionnaire(CCPAQ)which was designed for the assessment of physical activity pattern in young population in China.Methods The CCPAQ was administered for two times in 119 children(mean age 13.1±2.4 years;boys 47%)to examine reliability by using intraclass correlation coefficients.Validity was determined in 106 participants by agreement with the CCPAQ measures and the objective method,the ActiGraph accelerometer.Data on physical activity patter n including time spe nt on d iff ere nt intensi ties and total physical activity,sedentary behavior as well as physical activity energy expenditure were used to assess the validity with Spearman's correlation coefficient and the Bland-Altman plots.Results The reliability coefficient of the CCPAQ ranged from 0.63-0.93(Intra-class correlation coefficient).Spearman's correlation coefficient for validity of time spent on total physical activity and sedentary behavior were all 0.32(P<0.001),and for physical activity energy expenditure was 0.58(P<0.001).Time spent on moderate-to-vigorous physical activity and light physical activity showed a relatively low correlation with the accelerometer(rho=0.20,P=0.040;rho=0.19,P=0.054).Conclusion The CCPAQ appears to be a promising and feasible method to assess physical activity pattern in Chinese children. | YANG Xi JAGO Russell ZHAI Yi YANG Zhen Yu WANG Yu Ying SI Xiang WANG Jun GAO Jian Fen CHEN Jing Ron YU Ying Jie ZHAO Wen Hua | 2019 | Biomedical and Environmental Sciences2019，32，9： | 6 |
| 12 | Application of MPVR and TL-VR with 64-row MDCT in neonates with congenital EA and distal TEF显示文摘AIM:To assess the application of multiple planar volume reconstruction(MPVR) and three-dimensional (3D) transparency lung volume rendering(TL-VR) with 64-row multidetector-row computed tomography (MDCT) in neonates with congenital esophageal atresia (EA) and distal tracheoesophageal fistula(TEF).METHODS:Twenty neonates(17 boys,3 girls) with EA and distal TEF at a mean age of 4.6 d(range 1-16 d) were enrolled in this study.A helical scan of 64-row MDCT was performed at the 64 mm×0.625 mm collimation.EA and TEF were reconstructed with MPVR and TL-VR,respectively.Initial diagnosis of EA was made by chest radiography showing the inserted catheter in the proximal blind-ended esophageal pouch.Manifestations of MDCT images were compared with the findings at surgery.RESULTS:MDCT showed the proximal and distal esophageal pouches in 20 cases.No significant difference was observed in gaps between the proximal and distal esophageal pouches detected by MPVR and TLVR.The lengths of gaps between the proximal and distal esophageal pouches detected by MPVR and TL-VR correlated well with the findings at surgery(R=0.87,P<0.001).The images of MPVR revealed the orifice of TEF in 13 cases,while TL-VR images showed the orifice of TEF in 4 cases.CONCLUSION:EA and distal TEF can be reconstructed using MPVR and TL-VR of 64-row MDCT,which is a noninvasive technique to demonstrate the distal esophageal pouches and inter-pouch distance in neonates with EA and distal TEF. | Yang Wen Yun Peng Ren-You Zhai Ying-Zi Li | 2011 | World Journal of Gastroenterology2011，17，12： | 5 |
| 13 | Enhanced NO_2 gas sensing of a single-layer MoS_2 by photogating and piezo-phototronic effects显示文摘NO_2 sensors with ultrahigh sensitivity are demanded for future electronic sensing systems. However,traditional sensors are considerably limited by the relative low sensitivity, high cost and complicated process. Here, we report a simply and reliable flexible NO_2 sensor based on single-layer MoS_2. The flexible sensor exhibits high sensitivity to NO_2 gas due to ultra-large specific surface area and the nature of two-dimensional(2 D) semiconductor. When the NO_2 is 400 ppb(parts per billion), compared with the dark and strain-free conditions, the sensitivity of the single-layer sensor is enhanced to 671% with a625 nm red light-emitting diode(LED) illumination of 4 mW/cm^2 power under 0.67% tensile strain.More important, the response time is dramatically reduced to $16 s and it only needs $65 s to complete90% recovery. A theoretical model is proposed to discuss the microscopic mechanisms. We find that the remarkable sensing characteristics are the result of coupling among piezoelectricity, photoelectricity and adsorption-desorption induced charges transfer in the single-layer MoS_2 Schottky junction based device.Our work opens up the way to further enhancements in the sensitivity of gas sensor based on single-layer MoS_2 by introducing photogating and piezo-phototronic effects in mesoscopic systems. | Junmeng Guo Rongmei Wen Junyi Zhai Zhong Lin Wang | 2019 | Science Bulletin2019，64，2： | 5 |
| 14 | Consensus on the digestive endoscopic tunnel technique显示文摘With the digestive endoscopic tunnel technique(DETT), many diseases that previously would have been treated by surgery are now endoscopically curable by establishing a submucosal tunnel between the mucosa and muscularis propria(MP). Through the tunnel, endoscopic diagnosis or treatment is performed for lesions in the mucosa, in the MP, and even outside the gastrointestinal(GI) tract.At present, the tunnel technique application range covers the following:(1)Treatment of lesions originating from the mucosal layer, e.g., endoscopic submucosal tunnel dissection for oesophageal large or circular early-stage cancer or precancerosis;(2) treatment of lesions from the MP layer, per-oral endoscopic myotomy, submucosal tunnelling endoscopic resection, etc.; and(3) diagnosis and treatment of lesions outside the GI tract, such as resection of lymph nodes and benign tumour excision in the mediastinum or abdominal cavity. With the increasing number of DETTs performed worldwide, endoscopic tunnel therapeutics, which is based on DETT, has been gradually developed and optimized. However, there is not yet an expert consensus on DETT to regulate its indications, contraindications, surgical procedure, and postoperative treatment.The International DETT Alliance signed up this consensus to standardize the procedures of DETT. In this consensus, we describe the definition, mechanism,and significance of DETT, prevention of infection and concepts of DETTassociated complications, methods to establish a submucosal tunnel, and application of DETT for lesions in the mucosa, in the MP and outside the GI tract(indications and contraindications, procedures, pre-and postoperative treatments, effectiveness, complications and treatments, and a comparison between DETT and other operations). | Ning-Li Chai Hui-Kai Li En-Qiang Linghu Zhao-Shen Li Shu-Tian Zhang Yu Bao Wei-Gang Chen Philip WY Chiu Tong Dang Wei Gong Shu-Tang Han Jian-Yu Hao Shui-Xiang He Bing Hu1 Bing Hu2 Xiao-Jun Huang Yong-Hui Huang Zhen-Dong Jin Mouen A Khashab James Lau Peng Li Rui Li De-Liang Liu Hai-Feng Liu Jun Liu Xiao-Gang Liu Zhi-Guo Liu Ying-Cai Ma Gui-Yong Peng Long Rong Wei-Hong Sha Pateek Sharma Jian-Qiu Sheng Shui-Sheng Shi Dong Wan Seo Si-Yu Sun Gui-Qi Wang Wen Wang Qi Wu Hong Xu Mei-Dong Xu Ai-Ming Yang Fang Yao Hong-Gang Yu Ping-Hong Zhou Bin Zhang Xiao-Feng Zhang Ya-Qi Zhai | 2019 | World Journal of Gastroenterology2019，25，7： | 5 |
| 15 | Childhood BMI and Adult Obesity in a Chinese Sample:A 13-Year Follow-up Study显示文摘Objective Obesity is recognized as a significant risk factor for diabetes and hypertension. The present study aimed to examine the associations between adults' obesity risk and childhood and parental obesity. Methods A total of 204 children aged 6-17 years were recruited in 2002 with an average follow-up period of 13.2 years. Height and body weight were measured by trained staffs. Overweight and obesity were defined based on the Chinese standard for children and adults. T-test, analysis of variance, and Chi-square analysis were used for single factor analysis. Multiple linear and logistic regression analyses were used to perform multifactor analysis. Results The percentage of non-obese children who grew up to be non-obese adults was 62.6%, and that of obese children who grew up to be obese adults was 80.0%. There was a significant association between childhood body mass index(BMI) and adulthood BMI with a β regression coefficient of 3.76 [95% confidence interval(CI): 1.36-6.16], and between childhood obesity and adulthood obesity with an odds ratio of 5.76(95% CI: 1.37-24.34). There was no statistical difference between parental obesity at baseline and children's adulthood obesity, after adjustment of confounders. Male participants and those aged 10.0-13.0 years had a higher risk of adulthood obesity with odds ratios of 2.50(95% CI: 1.12-5.26) and 3.62(95% CI: 1.17-11.24), respectively. Conclusion Childhood obesity is an important predictor of adulthood obesity. | LIU Dan HAO Yun Xia ZHAO Ting Zhi SONG Peng Kun ZHAI Yi PANG Shao Jie ZHAO Yan Fang ZHANG Mei WANG Zhuo Qun MI Sheng Quan WANG Yu Ying ZHANG Jian ZHAO Wen Hua | 2019 | Biomedical and Environmental Sciences2019，32，3： | 5 |
| 16 | Growth performance of nursery and grower-finisher pigs fed diets supplemented with benzoic acid显示文摘Two experiments were conducted to investigate the efficacy of benzoic acid on the growth performance of nursery and grower-finisher pigs. A randomized complete block design was used in both experiments with the initial body weight as the blocking factor. There were 3 treatments corresponding to 3 dietary levels of benzoic acid: 0, 0.3%, and 0.5%. In experiment 1, a total of 144 PIC L1050 barrows(initial body weight 7.1 ± 0.6 kg) were used with each treatment replicated 8 times. In experiment 2, a total of 288 PIC L1050 barrows(initial body weight 36.1 ± 3.6 kg) were used with each treatment replicated 16 times.There were 6 barrows in each replicate pen for both experiments. Experiments 1 and 2 lasted 28 and 70 days, respectively. In experiment 1, average daily gain(ADG) of all growth phases increased linearly(P < 0.05) with increasing supplementation of benzoic acid, which led to a linear improvement in average body weight on d 28(P < 0.05). There was also an improvement in feed conversion ratio(FCR) of d 0 to 14(linear effect: P < 0.05) and in average daily feed intake(ADFI) of d 14 to 28 and d 0 to 28(linear effect:P < 0.01). In experiment 2, ADG during d 0 to 35 and d 35 to 70 and average body weight on d 35 improved linearly(P < 0.05) with increasing supplementation of benzoic acid. Average daily gain of d 0 to70 and average body weight on d 70 increased significantly in a both linear and quadratic manner. There was a linear improvement in FCR in all growth phases(P < 0.05). In conclusion, the dietary inclusion of benzoic acid at the supplementation levels of 0.3% and 0.5% significantly improved the growth performance of nursery and grower-finisher pigs in the current study; the nursery pigs responded to the dietary supplementation of benzoic acid up to 0.5% linearly while the grower-finisher pigs achieved the optimal ADG at the calculated supplementation level of 0.36%. | Hengxiao Zhai Wen Ren Shikui Wang Jinlong Wu Patrick Guggenbuhl Anna-Maria Kluenter | 2017 | Animal Nutrition2017，，3： | 4 |
| 17 | A preliminary investigation on genetic diversity of Sousa chinensis in the Pearl River Estuary and Xiamen of Chinese waters显示文摘In this study, the mitochondrial DNA (mtDNA) control region and the mitochondrial cytochrome b gene of stranded Indo-Pacific humpback dolphin (Sousa chinensis) samples from the Pearl River Estuary and Xiamen waters were sequenced and analyzed. The result of mtDNA control region revealed 34 variable sites and four unique haplotypes (named as A, B, C and D) identified among the total samples from these two water areas, and the most common haplotype (A) was shared by 75% of the dolphins sampled from the two water areas. The haplotypic diversity (h) was 0.455 and the nucleotide diversity (π) was 0.0088. The phylogenetic analysis showed that the hap- lotype A, C, and D were closely related, but the haplotype B (unique for XM01 from Xiamen) was far from the other three. By scanning cytochrome b fragments, two haplotypes (A and B) were identified in these two water areas, and the most common haplotype (A) was shared by 91.67% individuals, while XM01 from Xiamen as the only exception. The data suggest that there is a possibility of gene exchange between the two populations in the Pearl River Estuary and Xiamen waters, and there possibly exists a unique maternal lineage in Xiamen waters. | Hongshan Chen Kan Zhai Jialin Chen Yulong Chen Haijun Wen Sufang Chen Yuping Wu | 2008 | Journal of Genetics and Genomics2008，35，8： | 4 |
| 18 | A botanical medicine dragon’s blood exhibited clinical antithrombosis efficacy similar to low molecular weight heparin显示文摘Deep vein thrombosis(DVT)is a common complication following traumatic fracture with a 0.5%–1%annual incidence.Low molecular weight heparin(LMWH)is the most commonly used anticoagulation drug for DVT prevention,but treatment with LMWH is invasive.Our aim is to compare the antithrombotic effect of dragon’s blood,an oral botanical anticoagulant medicine approved by the Chinese FDA,with LMWH in patients undergoing hip fracture surgery and to explore the molecular mechanisms of anticoagulation treatment.Our study recruited patients and divided them into LMWH and dragon’s blood treatment group.Coagulation index tests,Doppler ultrasound and mRNA sequencing were performed before and after anticoagulation therapy.There was no significant difference in postoperative DVT incidence between the two groups(23.1%versus 15.4%,P=0.694).D-dimer(D-D)and fibrinogen degradation product(FDP)showed significant reductions in both groups after anticoagulation treatments.We identified SLC4A1,PROS1,PRKAR2B and seven other genes as being differentially expressed during anticoagulation therapy in both groups.Genes correlated with coagulation indexes were also identified.Dragon’s blood and LMWH showed similar effects on DVT and produced similar gene expression changes in patients undergoing hip fracture surgery,indicating that dragon’s blood is a more convenient antithrombosis medicine(oral)than LMWH(hypodermic injection). | Jiangbin Liang Song Mei Xiangyu Qiao Wei Pan Yan Zhao Shaohui Shi Yaling Zhai Haizhao Wen Guoping Wu Chengyu Jiang | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 19 | Global climate damage in 2℃ and 1.5℃ scenarios based on BCC_SESM model in IAM framework显示文摘The quantitative functions for climate damages provide theoretical ground for the cost-benefit analysis in climate change economics,and they are also critical for linking climate module with economic module in the Integrated Assessment Models(IAMs).Nevertheless,it is necessary for IAMs to update sectoral climate impacts in order to catch up the advance in climate change studies.This study updates the sectoral climate damage function at global scale from climate Framework for Uncertainty,Negotiation and Distribution(FUND)model and develops the aggregate climate damage function in a bottom-up fashion.Besides conventional sectors such as agriculture,forestry,water resources,energy consumption and ecosystems,this study expands climate disaster types,assesses human health impacts caused by various air pollutants,and updates coastal damage by sea level rise.The Beijing Climate Center Simple Earth System Model(BCC_SESM)is used to project climate system based on Business-as-Usual(BAU)scenario,and the 2℃ and 1.5℃ scenarios based on RCPs and SSP2 databases.Sectoral results show that the agricultural sector is projected to suffer 63% of the total damage,followed by water resources(16%)and human health(12%)sectors in 2100.The regression results indicate that the aggregate climate damage function is in positive quadratic form for zero discounting.Under BAU scenario,the aggregate climate damage is projected to be 517.7 trillion USD during 2011-2100.Compared to that,the 2℃ and 1.5℃ scenarios are projected to respectively reduce climate damages by 215.6 trillion USD(approximately 41.6%)and 263.5 trillion USD(50.9%)in 2011-2100. | ZHAO Zi-Jian CHEN Xiao-Tong LIU Chang-Yi YANG Fang TAN Xin ZHAO Yang HUANG Han WEI Chao SHI Xue-Li ZHAI Wen GUO Fei Bas J.VAN RUIJVEN | 2020 | Advances in Climate Change Research2020，11，3： | 4 |
| 20 | Optimization of the Ultrasonic-assisted Extraction Process for Protocatechuic Acid from Emilia sonchifolia DC by Response Surface Methodology显示文摘[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/ml.The experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results. | Wen CHEN Xiangjun WANG Lingyan LI Zhewen ZHAI Xiaoling HUANG Wubing RAO Chenghan ZOU Tengtong LI | 2020 | Agricultural Biotechnology2020，9，3： | 3 |
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