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| 1 | A 4-bp Insertion at ZmPLA 1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize显示文摘 | Chenxu Liu Xiang Li Dexuan Meng Yu Zhong Chen Chen Xin Dong Xiaowei Xu Baojian Chen Wei Li Liang Li Xiaolong Tian Haiming Zhao Weibin Song Haishan Luo Qinghua Zhang Jinsheng Lai Weiwei Jin Jianbing Yan Shaojiang Chen | 2017 | Molecular Plant2017，10，3： | 39 |
| 2 | Crop Phenomics and High-Throughput Phenotyping:Past Decades,Current Challenges,and Future Perspectives显示文摘Since whole-genome sequencing of many crops has been achieved,crop functional genomics studies have stepped into the big-data and high-throughput era.However,acquisition of large-scale phenotypic data has become one of the major bottlenecks hindering crop breeding and functional genomics studies.Nevertheless,recent technological advances provide us potential solutions to relieve this bottleneck and to explore advanced methods for large-scale phenotyping data acquisition and processing in the coming years.In this article,we review the major progress on high-throughput phenotyping in controlled environments and field conditions as well as its use for post-harvest yield and quality assessment in the past decades.We then discuss the latest multi-omics research combining high-throughput phenotyping with genetic studies.Finally,we propose some conceptual challenges and provide our perspectives on how to bridge the phenotype-genotype gap.It is no doubt that accurate high-throughput phenotyping will accelerate plant genetic improvements and promote the next green revolution in crop breeding. | Wanneng Yang Hui Feng Xuehai Zhang Jian Zhang John H.Doonan William David Batchelor Lizhong Xiong Jianbing Yan | 2020 | Molecular Plant2020，13，2： | 38 |
| 3 | Glucocorticoid receptor and sequential P53 activation by dexamethasone mediates apoptosis and cell cycle arrest of osteoblastic MC3T3-E1 cells显示文摘 | Li Hui Yao Jianfeng Liu Hui Liu Shuguang Xu Peng Ma Jianbing Zhang Yumin | 2012 | 美中国际创伤杂志2012，11，1： | 22 |
| 4 | Development of highway constructing technology in the permafrost region on the Qinghai-Tibet plateau显示文摘The Qinghai-Tibet Highway passes through the Qinghai-Tibet plateau hinterland from north to south, from Kunlun Mountain to Tanggula Mountain. The average altitude is above 4500 m and permafrost covers more than 700 km length of area with high elevation and high temperature. The climate of the plateau is capricious and the surroundings along the road are bad, where the mean annual atmosphere temperature is -2 to -7℃ and the oxygen content is inadequate, which is less than 50% of the sea level, while the solar radiation is higher than 3600 kJ/m2. The basic characteristics of the plateau surroundings are the permafrost, coldness with litter oxygen, and fragile ecosystem. As the air temperature warms all over the world, the permafrost on the plateau responds quickly. The average temperature of permafrost is up by 0.2―0.3℃ in recent 20 years, and the island-permafrost dwindles with a high rate, and the high temperature permafrost degrades quickly, and the temperature of the low temperature permafrost increases remarkably. These gradually cause the highway engineering diseases in the permafrost region. The Qinghai-Tibet highway has experienced much maintenance and rebuilding for 50 years after it was built, and the continual observation and investigation have been made for more than 30 years. This road is the longest test engineering for permafrost research work in China. It is no doubt that it is also the greatest engineering project in plateau permafrost region. The Ministry of Communication in China initiated the research project 'Research on a series of technologies for highway constructing in the permafrost regions' in 2002, which was to meet the challenges from the effect of climatic warming on the permafrost. The project systematically studied the permafrost engineering theories, methods for survey and design, engineering stabilization measures, preventing of the road disasters and maintaining, environment protection and the techniques to prolong the road engineering life in the cold surroundings with high altitude and less oxygen. This article describes a series of technologies for highway constructing according to the special surroundings in the plateau, including some innovative achievements and the research and development of permafrost engineering. | WANG ShuangJie CHEN JianBing ZHANG JinZhao LI ZhuLong | 2009 | Science China(Technological Sciences)2009，52，2： | 16 |
| 5 | The HuangZaoSi Maize Genome Provides Insights into Genomic Variation and Improvement History of Maize显示文摘Maize is a globally important crop that was a classic model plant for genetic studies. Here, we report a 2.2 Gb draft genome sequence of an elite maize line, HuangZaoSi (HZS). Hybrids bred from HZS-improved lines (HILs) are planted in more than 60% of maize fields in China. Proteome clustering of six completed sequeneed maize genomes show that 638 proteins fall into 264 HZS-specific gene families with the majority of contributions from tandem duplication events. Resequencing and comparative analysis of 40 HZSrelated lines reveals the breeding history of HILs. More than 60% of identified selective sweeps were clustered in identity.by.descent conserved regions, and yield-related genes/QTLs were enriched in HZS characteristic selected regions. Furthermore, we dem on strated that HZS-specific family genes were not uniformly distributed in the genome but enriched in improvement/function.related genomic regions. This study provides an important and novel resource for maize genome research and expands our knowledge on the breadth of genomic variation and improvement history of maize. | Chunhui Li Wei Song Yingfeng Luo Shenghan Gao Ruyang Zhang Zi Shi Xiaqing Wang Ronghuan Wang Fengge Wang Jidong Wang Yanxin Zhao Aiguo Su Shuai Wang Xin Li Meijie Luo Shuaishuai Wang Yunxia Zhang Jianrong Ge Xinyu Tan Ye Yuan Xiaochun Bi Hang He Jianbing Yan Yuandong Wang Songnian Hu Jiuran Zhao | 2019 | Molecular Plant2019，12，3： | 15 |
| 6 | ZmCOL3, a CCT gene represses flowering in maize by interfering with the circadian clock and activating expression of ZmCCT显示文摘Flowering time is a trait vital to the adaptation of flowering plants to different environments.Here, we report that CCT domain genes play an important role in flowering in maize(Zea mays L.).Among the 53 CCT family genes we identified in maize, 28 were located in flowering time quantitative trait locus regions and 15 were significantly associated with flowering time, based on candidate-gene association mapping analysis.Furthermore, a CCT gene named ZmCOL3 was shown to be a repressor of flowering.Overexpressing ZmCOL3 delayed flowering time by approximately 4 d, in either long-day or short-day conditions.The absence of one cytosine in the ZmCOL3 3'UTR and the presence of a 551 bp fragment in the promoter region are likely the causal polymorphisms contributing to the maize adaptation from tropical to temperate regions.We propose a modified model of the maize photoperiod pathway, wherein ZmCOL3 acts as an inhibitor of flowering either by transactivating transcription of ZmCCT, one of the key genes regulating maize flowering, or by interfering with the circadian clock. | Minliang Jin Xiangguo Liu Wei Jia Haijun Liu Wenqiang Li Yong Peng Yanfang Du Yuebin Wang Yuejia Yin Xuehai Zhang Qing Liu Min Deng Nan Li Xiyan Cui Dongyun Hao Jianbing Yan | 2018 | Journal of Integrative Plant Biology2018，60，6： | 14 |
| 7 | Distant eQTLs and Non-coding Sequences Play Critical Roles in Regulating Gene Expression and Quantitative Trait Variation in Maize显示文摘 | Haijun Liu Xin Luo Luyao Niu Yingjie Xiao Lu Chen Jie Liu Xiaqing Wang Minliang Jin Wenqiang Li Qinghua Zhang Jianbing Yan | 2017 | Molecular Plant2017，10，3： | 13 |
| 8 | Detrital K-feldspar^(40)Ar/^(39)Ar Ages:Source Constraints of the Lower Miocene Sandstones in the Pearl River Mouth Basin,South China Sea显示文摘The South China Sea began to outspread in the Oligocene.A great quantity of terraneous detritus was deposited in the northern continental shelf of the sea,mostly in Pearl River Mouth Basin,which constituted the main paleo-Pearl River Delta.The delta developed for a long geological time and formed a superimposed area.Almost all the oil and gas fields of detrital rock reservoir distribute in this delta.Thirty-three oil sandstone core samples in the Zhujiang Formation,lower Miocene(23-16 Ma),were collected from nine wells.The illite samples with detrital K feldspar(Kfs) separated from these sandstone cores in four sub-structural belts were analysed by the high-precision 40Ar/39Ar laser stepwise heating technique.All 33 illite 40Ar/39Ar data consistently yielded gradually rising age spectra at the low-temperature steps until reaching age plateaus at mid-high temperature steps.The youngest ages corresponding to the beginning steps were interpreted as the hydrocarbon accumulation ages and the plateau ages in mid-high temperature steps as the contributions of the detrital feldspar representing the ages of the granitic parent rocks in the provenances.The ages of the detrital feldspar from the Zhujiang Formation in the four sub-structural belts were different:(1) the late Cretaceous ages in the Lufeng 13 fault structural belt;(2) the late Cretaceous and early Cretaceous-Jurassic ages in the Huizhou 21 buried hill-fault belt;(3) the Jurassic and Triassic ages in the Xijiang 24 buried hill-fault belt;and(4) the early Cretaceous-late Jurassic ages in the Panyu 4 oil area.These detrital feldspar 40Ar/39Ar ages become younger and younger from west to east,corresponding to the age distribution of the granites in the adjacent Guangdong Province,Southern China. | LIU Zaisheng SHI Hesheng ZHU Junzhang QIU Huaning ZHANG Zhilin YUN Jianbing | 2012 | Acta Geologica Sinica(English Edition)2012，86，2： | 9 |
| 9 | Genotype variations in accumulation of cadmium and lead in celery (Apium graveolens L.) and screening for low Cd and Pb accumulative cultivars显示文摘To help reduce risks of heavy metal pollution, two pot experiments were conducted to investigate the variations, transfer potential, and stability of Cadmium (Cd) and Lead (Pb) accumulations in celery (Apium graveolens L.) and to screen for low Cd and Pb accumulative cultivars. The maximum differences in shoot Cd concentration were 4.7-fold under low-Cd exposure and 3.3-fold under high-Cd exposure. These genotype variations in Cd accumulation are sufficiently large to help reduce Cd contamination risk in soil by using the Low-Cd-Accumulative genotypes. Cd accumulation of the Low-Cd-Accumulative genotypes is significantly positive correlated with Pb accumulation. Evidence obtained proves that Cd and Pb accumulations in celery are stable and genotype-dependent at the cultivar level. The presence of high-Pb contamination in soil promoted Cd accumulation in shoots of celery. Celery is considered a species with high risks in Cd pollution and low risks in Pb pollution. Among the tested cultivars, cv. Shuanggang- kangbing (SGKB) had the lowest shoot Cd and Pb accumulating abilities, and thus is the most important material for breeding of pollution-safe cultivars (PSCs) to minimize Cd and Pb accumulations in celery. | Kun ZHANG Jianbing WANG Zhongyi YANG Guorong XIN Jiangang YUAN Junliang XIN Charlie HUANG | 2013 | Frontiers of Environmental Science & Engineering2013，7，1： | 9 |
| 10 | Effect of wet flue gas desulfurization(WFGD) on fine particle(PM_(2.5)) emission from coal-fired boilers显示文摘In this study, the characteristics of fine particles before and after wet flue gas desulfurization(WFGD) in three coal-fired heating boilers in northern China were investigated by using a dilution-based emission sampling experimental system. The influences of the WFGD process on the mass and number concentrations as well as the chemical composition of fine particles were analyzed. The removal efficiency of desulfurization processes on particulate matter mass was 30.06%–56.25% for the three study units. The WFGD had a great influence on the size distributions of particle mass concentration and number concentration. A significant increase in the number and mass concentration of particles in the size range of 0.094–0.946 μm was observed. The watersoluble ion content accounted for a very large proportion of PM_(2.5) mass, and its proportion in PM_(2.5) increased from 28.39%–41.08% to 48.96%–61.21% after the WFGD process for the three units. The desulfurizing process also drastically increased the proportion of cation component(Ca^(2+) for unit A, Mg^(2+) for unit B, and Na+for unit C) and the proportion of SO_4^(2-) in PM_(2.5), and it increased the CE/AE values of PM_(2.5) from 0.82–0.98 to 0.93–1.27 for the three study units. | Sen Yao Shuiyuan Cheng Jianbing Li Hanyu Zhang Jia Jia Xiaowei Sun | 2019 | Journal of Environmental Sciences2019，31，3： | 8 |
| 11 | Identification of unconditional and conditional QTL for oil, protein and starch content in maize显示文摘Oil, protein and starch are key chemical components of maize kernels. A population of 245 recombinant inbred lines(RILs) derived from a cross between a high-oil inbred line, By804, and a regular inbred line, B73, was used to dissect the genetic interrelationships among oil, starch and protein content at the individual QTL level by unconditional and conditional QTL mapping. Combined phenotypic data over two years with a genetic linkage map constructed using 236 markers, nine, five and eight unconditional QTL were detected for oil, protein and starch content, respectively. Some QTL for oil, protein and starch content were clustered in the same genomic regions and the direction of their effects was consistent with the sign of their correlation. In conditional QTL mapping, 37(29/8) unconditional QTL were not detected or showed reduced effects, four QTL demonstrated similar effects under unconditional and conditional QTL mapping, and 17 additional QTL were identified by conditional QTL mapping. These results imply that there is a strong genetic relationship among oil, protein and starch content in maize kernels. The information generated in the present investigation could be helpful in marker-assisted breeding for maize varieties with desirable kernel quality traits. | Yuqiu Guo Xiaohong Yang Subhash Chander Jianbing Yan Jun Zhang Tongming Song Jiansheng Li | 2013 | The Crop Journal2013，1，1： | 7 |
| 12 | BRIF-Seq: Bisulfite-Converted Randomly Integrated Fragments Sequencing at the Single-Cell Level显示文摘Single-cell bisulfite sequencing (scBS-seq) was developed to assess DNA methylation heterogeneity in human and mouse. However, the reads are under-represented in regions with high DNA methylation, because these regions are usually fragmented into long segments and are seldom sequenced on the lllumina plat. form. To reduce the read distribution bias and maximize the use of these long segments, we developed bisulfite-converted randomly integrated fragments sequencing (BRIF-seq), a method with high rates of read mapping and genome coverage. Single microspore of maize, which has a highly methylated and repetitive genome, was used to perform BRIF.seq. High coverage of the haploid genome was obtained to evaluate the methylation states of CG, CHG, and CHH (H = A, C, or T). Compared with scBS-seq, BRIF-seq produced reads that were distributed more evenly across the genome, including regions with high DNA methylation. Surprisingly, the methylation rates among the four microspores within one tetrad were similar, but differed significantly among tetrads, suggesting that non-simultaneous methylation reprogramming could occur among tetrads. Similar levels of heterogeneity, which often occur in lowcopy regions, were detected in different genetic backgrounds. These results suggest that BRIF-seq can be applied for single-cell methylome analysis of any species with diverse genetic backgrounds. | Xiang Li Lu Chen Qinghua Zhang Yonghao Sun Qing Li Jianbing Yan | 2019 | Molecular Plant2019，12，3： | 7 |
| 13 | Enhanced Vitamin C Production Mediated by an ABA-Induced PTP-like Nucleotidase Improves Plant Drought Tolerance in Arabidopsis and Maize显示文摘Abscisic acid(ABA)is a key phytohormone that mediates environmental stress responses.Vitamin C,or L-ascorbic acid(AsA),is the most abundant antioxidant protecting against stress damage in plants.How the ABA and AsA signaling pathways interact in stress responses remains elusive.In this study,we characterized the role of a previously unidentified gene,PTPN(PTP-like Nucleotidase)in plant drought tolerance.In Arabidopsis,(AtPTPN was expressed in multiple tissues and upregulated by ABA and drought treatments.Loss-of-function mutants oiAtPTPN were hyposensitive to ABA but hypersensitive to drought stresses,whereas plants with enhanced expression AtPTPN showed opposite phenotypes to.Overexpression of maize PTPN(ZmPTPN)promoted,while knockdown oiZmPTPN inhibited plant drought tolerance,indicating conserved and positive roles of PTPN in plant drought tolerance.We found that both AtPTPN and ZmPTPN release Pi by hydrolyzing GDP/GMP/dGMP/IMP/dIMP,and that AtPTPN positively regulated AsA production via endogenous Pi content control.Consistently,overexpression of VTC2,the rate-limiting synthetic enzyme in AsA biosynthesis,promoted AsA production and plant drought tolerance,and these effects were largely dependent on AtPTPN activity.Furthermore,we demonstrated that the heat shock transcription factor HSFA6a directly binds the AtPTPN promoter and activates AtPTPN expression.Genetic analyses showed that AtPTPN is required for HSFA6a to regulate ABA and drought responses.Taken together,our data indicate that PTPN-mediated crosstalk between the ABA signaling and AsA biosynthesis pathways positively controls plant drought tolerance. | Hui Zhang Yanli Xiang Neng He Xiangguo Liu Hongbo Liu Liping Fang Fei Zhang Xiaopeng Sun Delin Zhang Xingwang Li William Terzaghi Jianbing Yan Mingqiu Dai | 2020 | Molecular Plant2020，13，5： | 7 |
| 14 | Gram-scale synthesis of all-inorganic perovskite quantum dots with high Mn substitution ratio and enhanced dual-color emission显示文摘Mn-doped all-inorganic perovskite quantum dots (QDs) provide prominent applications in the fields of low-cost light source or display,because of their remarkable properties including dual-color emission and reduced lead content,as well as high photoluminescence quantum yields (PLQYs) and high stability.However,the existing synthesis approaches usually require hash conditions,such as high temperature and nitrogen protection,which is a major hurdler for the practical manufacturing.In addition,the significantly high Mn substitution ratio in CsPbX3 QDs is still challenging.The real dual-color emission with two strong emission peaks in the Mn-doped all-inorganic perovskite QDs has attracted great interest.Here we present a gram-scale approach to synthesize both CsPbxMn1-xCI3 and CsPb1-xMnxClyBr3-y QDs at 100 ℃ in the air with high Mn substitution ratio,up to 55.64% atomically.The as-prepared CsPb1-xMnxClyBr3-y QDs exhibit high PLQYs of 62.41% and dual-color emission with two strong emission peaks around at 400-450 nm and 600 nm,respectively.The enhanced peak at 400-450 nm is a result of the hybrid halides in CsPbBrxCl3-x host.Furthermore,the unique advantage of the optical emission and high PLQYs properties of the CsPbxMn1-xCl3 QDs has been demonstrated as invisible ink for encryption applications and polymer composites.Our gram-scale synthesis approach for Mn-doped all-inorganic perovskite QDs may boost the future research and practical applications of QDs-based white LED,spintronics,and molecular barcoding. | Lvming Dong Zhuo Chen Lei Ye Yan Yu Jianbing Zhang Huan Liu Jianfeng Zang | 2019 | Nano Research2019，12，7： | 6 |
| 15 | The RppC-AvrRppC NLR-effector interaction mediates the resistance to southern corn rust in maize显示文摘Southern corn rust(SCR),caused by the fungal pathogen Puccinia polysora,is a major threat to maize pro-duction worldwide.Efficient breeding and deployment of resistant hybrids are key to achieving durable control of SCR.Here,we report the molecular cloning and characterization of RppC,which encodes an NLR-type immune receptor and is responsible for a major SCR resistance quantitative trait locus.Further-more,we identified the corresponding avirulence effector,AvrRppC,which is secreted by P.polysora and triggers RppC-mediated resistance.Allelic variation of AvrRppC directly determines the effectiveness of RppC-mediated resistance,indicating that monitoring of AvrRppC variants in the field can guide the rational deployment of RppC-containing hybrids in maize production.Currently,RppC is the most frequently deployed SCR resistance gene in China,and a better understanding of its mode of action is crit-ical for extending its durability. | Ce Deng April Leonard James Cahill Meng Lv Yurong Li Shawn Thatcher Xueying Li Xiaodi Zhao Wenjie Du Zheng Li Huimin Li Victor Llaca Kevin Fengler Lisa Marshall Charlotte Harris Girma Tabor Zhimin Li Zhiqiang Tian Qinghua Yang Yanhui Chen Jihua Tang Xintao Wang Junjie Hao Jianbing Yan Zhibing Lai Xiaohong Fei Weibin Song Jinsheng Lai Xuecai Zhang Guoping Shu Yibo Wang Yuxiao Chang Weiling Zhu Wei Xiong Juan Sun Bailin Li Junqiang Ding | 2022 | Molecular Plant2022，15，5： | 6 |
| 16 | Alteration of the microRNA expression profile and identification of miRNA/mRNA negative regulation pairs in neural tube defects显示文摘MicroRNAs (miRNAs) are small, noncoding transcripts, and ~22 nucleotides long, which belong to a class of endogenous small regulatory RNA molecules that target mRNAs and trigger either translational suppression or mRNA degradation [1–3]. Several studies have shown that miRNAs play crucial roles in neurogenesis and development of nervous system [4,5] and have distinct expression patterns within the developing brain and central nervous system (CNS)[6]. Abnormal expression of miRNAs has also been reported in dysregulated neural tube (NT) development including NT defects (NTDs)[7,8]. To date, however, all NTD-related miRNA profiles were obtained from different microarray platforms, which always contain limited probes, and the data obtained are less precise in quantitative analysis. No quantitatively profiled global miRNA expression in mouse NTD fetuses has been characterized so far. To gain further insight of the possible roles of miRNAs in NTDs, we used deep sequencing to carry out a comprehensive survey of miRNA expression in all-trans retinoic acid (RA)-induced NTD mouse fetuses collected from three different embryonic time points (E8.5, E9.5, and E10.5). This study is the first, to our knowledge, to report the deep sequencing miRNA profile, which defined unique gene expression signatures of a range of miRNAs and their potential regulatory networks in response to RA stress and the development of NTDs in mouse model. | Juan Zhang Lihong Yang Juan Yu Qiaoyan Yang Jianbing Mu Jun Xie | 2019 | Acta Biochimica et Biophysica Sinica2019，51，7： | 6 |
| 17 | Treatment of coking wastewater using oxic-anoxic-oxic process followed by coagulation and ozonation显示文摘An oxic-anoxic-oxic(O-A-O)system followed by coagulation and ozonation processes was used to study the treatment of coking wastewater.In the O-A-O process,the removals of NH4+-N,total nitrogen and COD were 91.5-93.3%,91.3-92.6%and 89.1-93.8%,respectively when employing hydraulic residence times of 60 h for the biochemical system.High removal of NH4+-N was obtained due to the placement of an aerobic tank in front of A-O system which can mitigate the inhibitory effect of toxic compounds in coking wastewater on nitrifying bacteria.Addition of methanol into the anoxic reactor greatly increased the removal of total nitrogen,indicating that denitrifiers can hardly use organic compounds in coking wastewater as carbon source for denitrification.COD values of the effluent from the O-A-O system were still higher than 260 mg/L even with a prolonged time of 160 h mainly due to the high refractory properties of residual compounds in the effluent.The subsequent coagulation and ozonation processes resulted in the COD removal of 91.5%-93.3%and reduced the relative abundance of large molecular weight(MW)organics(>1 kDa)from 55.8%to 20.93%with the ozone,PAC and PAM dosages of 100,150 and 4 mg/L respectively.Under these conditions,the COD value and concentration of polycyclic aromatic hydrocarbons in the final effluent were less than 80 and 0.05 mg/L,respectively,which meet the requirement of the Chinese emission standard.These results indicate that the combined technology of O-A-O process,coagulation and ozonation is a reliable way for the treatment of coking wastewater. | Jianbing Wang Yuxian Ji Fengyuan Zhang Dongliang Wang Xuwen He Chunrong Wang | 2019 | Carbon Resources Conversion2019，2，2： | 5 |
| 18 | The Distribution of Dissolved Aluminum in the Yellow and East China Seas显示文摘Water samples containing dissolved aluminum were collected from the Yellow and East China Seas in Octo- ber-November 2000. The average concentrations of dissolved Al in the Yellow Sea (YS) and East China Sea (ECS) were 0.042 and 0.056 μmolL-1, respectively. The concentration of dissolved aluminum decreased gradually across the continental shelf. The lower concentrations appeared in the YS cold water center and in the bottom layer at the shelf edge of the ECS, where they were 0.016 and 0.011 μmolL-1, respectively. The distribution of dissolved Al was controlled by physical mixing processes rather than biological up- take processes. The impact of different water masses along the PN transect was calculated based on the mass balance model. The results show that the impact of the Changjiang River was mainly concentrated on the coastal area and the top thermocline water on the ECS shelf, where the impact percentage decreased from 12.6% to 1.1% in the surface water, while the contribution of the Ku- roshio water was dominant on the ECS shelf in this survey, increasing from 77.6% to 97.8% along the PN transect from the Changjiang River Estuary to the Ryukyu Islands. It is concluded that aluminum can serve as a proper tracer for studying the impact of Changjiang terrestrial matter on the ECS shelf water. | LI Jianbing REN Jingling ZHANG Jing LIU Sumei | 2008 | Journal of Ocean University of China2008，7，1： | 5 |
| 19 | Large-Scale Discovery of Non-conventional Peptides in Maize and Arabidopsis through an Integrated Peptidogenomic Pipeline显示文摘Non-conventional peptides(NCPs),which include small open reading frame-encoded peptides,play critical roles in fundamental biological processes.In this study,we developed an integrated peptidogenomic pipeline using high-throughput mass spectra to probe a customized six-frame translation database and applied it to large-scale identification of NCPs in plants.A total of 1993 and 1860 NCPs were unambiguously identified in maize and Arabidopsis,respectively.These NCPs showed distinct characteristics compared with conventional peptides and were derived from introns,3′UTRs,5′UTRs,junctions,and intergenic regions.Furthermore,our results showed that translation events in unannotated transcripts occur more broadly than previously thought.In addition,we found that dozens of maize NCPs are enriched within regions associated with phenotypic variations and domestication selection,indicating that they potentially are involved in genetic regulation of complex traits and domestication in maize.Taken together,our study developed an integrated peptidogenomic pipeline for large-scale identification of NCPs in plants,which would facilitate global characterization of NCPs from other plants.The identification of large-scale NCPs in both monocot(maize)and dicot(Arabidopsis)plants indicates that a large portion of plant genome can be translated into biologically functional molecules,which has important implications for functional genomic studies. | Shunxi Wang Lei Tian Haijun Liu Xiang Li Jinghua Zhang Xueyan Chen Xingmeng Jia Xu Zheng Shubiao Wu Yanhui Chen Jianbing Yan Liuji Wu | 2020 | Molecular Plant2020，13，7： | 4 |
| 20 | Hydrodynamic cavitation as an efficient method for the formation of sub-100 nm O/W emulsions with high stability显示文摘Hydrodynamic cavitation,a newly developed process intensification technique,has demonstrated immense potential for intensifying diverse physical and chemical processes.In this study,hydrodynamic cavitation was explored as an efficient method for the formation of sub-100 nm oil-in-water(O/W) emulsions with high stability.O/W emulsion with an average droplet size of 27 nm was successfully prepared.The average droplet size of O/W emulsions decreased with the increase of the inlet pressure,number of cavitation passes and surfactant concentration.The formed emulsion exhibited admirable physical stability during 8 months.Moreover,the hydrodynamic cavitation method can be generalized to fabricate large varieties of O/W emulsions,which showed great potential for large-scale formation of O/W emulsions with lower energy consumption. | Zhiliang Zhang Guangquan Wang Yong Nie Jianbing Ji | 2016 | Chinese Journal of Chemical Engineering2016，24，10： | 4 |
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