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| 1 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
| 2 | Changes in energy metabolism in the quadriceps femoris after a single bout of acute exhaustive swimming in rats: a 31p-magnetic resonance spectroscopy study显示文摘 | Sun Yingwei Pan Shinong Chen Zhian Zhao Heng Ma Ying Zheng Liqiang Li Qi Deng Chunbo Fu Xihu Lu Zaiming Guo Qiyong | 2014 | Chinese Medical Journal2014，，5： | 5 |
| 3 | Implementation of regional Acute Stroke Care Map increases thrombolysis rates for acute ischaemic stroke in Chinese urban area in only 3 months显示文摘Background The rate of intravenous thrombolysis for acute ischaemic stroke remains low in China.We investigated whether the implementation of a citywide Acute Stroke Care Map(ASCaM)is associated with an improvement of acute stroke care quality in a Chinese urban area.Methods The ASCaM comprises 10 improvement strategies and has been implemented through a network consisting of 20 tertiary hospitals.We identified 7827 patients with ischaemic stroke admitted from April to October 2017,and 506 patients underwent thrombolysis were finally included for analysis.Results Compared with‘pre-ASCaM period’,we observed an increased rate of administration of tissue plasminogen activator within 4.5 hours(65.4% vs 54.5%;adjusted OR,1.724;95% CI 1.21 to 2.45;p=0.003)during‘ASCaM period’.In multivariate analysis models,‘ASCaM period’was associated with a significant reduction in onset-to door time(114.1±55.7 vs 135.7±58.4 min,p=0.0002)and onset-to needle time(ONT)(169.2±58.1 vs 195.6±59.3 min,p<0.0001).Yet no change was found in door-to needle time.Clinical outcomes such as symptomatic intracranial haemorrhage,favourable functional outcome(modified Rankin Scale≤2)and in-hospital mortality remained unchanged.Conclusion The implementation of ASCaM was significantly associated with increased rates of intravenous thrombolysis and shorter ONT.The ASCaM may,in proof-of principle,serve as a model to reduce treatment delay and increase thrombolysis rates in Chinese urban areas and possibly other highly populated Asian regions. | Yi Sui Jianfeng Luo Chunyao Dong Liqiang Zheng Weijin Zhao Yao Zhang Ying Xian Huaguang Zheng Bernard Yan Mark Parsons Li Ren Ying Xiao Haoyue Zhu Lijie Ren Qi Fang Yi Yang Weidong Liu Bing Xu | 2021 | Stroke & Vascular Neurology2021，6，1： | 4 |
| 4 | Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China显示文摘Dear Editor,Rift Valley fever(RVF)is an anthropozoonosis caused by Rift Valley fever virus(RVFV).RVFV belongs to the Phlebovirus genus in the family Bunyaviridae,which is circulating among ruminants.Human infection with RVFV is generally asymptomatic,however,minority of patients develop severe RVF diseases like encephalitis | Yongxia Shi Kui Zheng Xiaobo Li Liqiang Li Shufen Li Jinmin Ma Jun Dai Jingkai Ji Shuai Yuan Haorong Lu Jiandong Li Fangfang Sun Xun Xu Jicheng Huang | 2017 | Virologica Sinica2017，32，3： | 4 |
| 5 | Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury显示文摘Spinal cord injury(SCI)involves diverse injury responses in different cell types in a temporally and spatially specific manner.Here,using single-cell transcriptomic analyses combined with classic anatomical,behavioral,electrophysiological analyses,we report,with single-cell resolution,temporal molecular and cellular changes in crush-injured adult mouse spinal cord.Data revealed pathological changes of 12 different major cell types,three of which infiltrated into the spinal cord at distinct times post-injury.We discovered novel microglia and astrocyte subtypes in the uninjured spinal cord,and their dynamic conversions into additional stage-specific subtypes/states.Most dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged,accompanied with changes in various cell types including neurons,indicative of the second round of attacks.By day-38,major cell types are still substantially deviated from uninjured states,demonstrating prolonged alterations.This study provides a comprehensive mapping of cellular/molecular pathological changes along the temporal axis after SCI,which may facilitate the development of novel therapeutic strategies,including those targeting microglia. | Chen Li Zhourui Wu Liqiang Zhou Jingliang Shao Xiao Hu Wei Xu Yilong Ren Xingfei Zhu Weihong Ge Kunshan Zhang Jiping Liu Runzhi Huang Jing Yu Dandan Luo Xuejiao Yang Wenmin Zhu Rongrong Zhu Changhong Zheng Yi Eve Sun Liming Cheng | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 4 |
| 6 | Effects of biochar amendment on soil quality, crop yield and greenhouse gas emission in a Chinese rice paddy: A field study of 2 consecutive rice growing cycles显示文摘 | Afeng Zhang Rongjun Bian Genxing Pan Liqiang Cui Qaiser Hussain Lianqing Li Jinwei Zheng Jufeng Zheng Xuhui Zhang Xiaojun Han Xinyan Yu | 2011 | Field Crops Research2011，，： | 4 |
| 7 | Effect of biochar amendment on yield and methane and nitrous oxide emissions from a rice paddy from Tai Lake plain, China显示文摘 | Afeng Zhang Liqiang Cui Gengxing Pan Lianqing Li Qaiser Hussain Xuhui Zhang Jinwei Zheng David Crowley | 2010 | Agriculture Ecosystems and Environment2010，，4： | 3 |
| 8 | Effect of KBr on the micellar properties of CTAB显示文摘The effect of KBr on the size, shape and microviscosity of CTAB micelles has been investigated by means of laser light scattering (LLS), 1H NMR measurements and fluorescence probe. The data obtained from the various techniques are quantitatively in agreement. The Rh of micelles in 0.01 mol ·L-1 CTAB solution increases from 3.5 nm to 43 nm and Rg increases to 89 nm with addition of KBr salt. In this process, both the microviscosity and molecular weight of micelles Mw have noticeable increases, too. The rod-like micelles are formed at 0.1 mol ·L -1 KBr and the worm-like micelles are formed at above 0.2 mol ·L-1 KBr. | ZHANG Weican LI Ganzuo MU Jianhai SHEN Qiang ZHENG Liqiang LIANG Haojun WU Chi | 2000 | Chinese Science Bulletin2000，45，20： | 3 |
| 9 | Effect of biochar amendment on yield and methane and nitrous oxide emissions from a rice paddy from Tai Lake plain, China显示文摘 | Afeng Zhang Liqiang Cui Gengxing Pan Lianqing Li Qaiser Hussain Xuhui Zhang Jinwei Zheng David Crowley | 2010 | Agriculture Ecosystems and Environment2010，，4： | 3 |
| 10 | Effect of biochar amendment on yield and methane and nitrous oxide emissions from a rice paddy from Tai Lake plain, China显示文摘 | Afeng Zhang Liqiang Cui Gengxing Pan Lianqing Li Qaiser Hussain Xuhui Zhang Jinwei Zheng David Crowley | 2010 | Agriculture, Ecosystems and Environment2010，，4： | 2 |
| 11 | Aqueous Cr(VI) photo-reduction catalyzed by TiO 2 and sulfated TiO 2显示文摘 | Fang Jiang Zheng Zheng Zhaoyi Xu Shourong Zheng Zhaobing Guo Liqiang Chen | 2005 | Journal of Hazardous Materials2005，，1： | 1 |
| 12 | Prevalence of Dyslipidemia and Associated Factors Among the Hypertensive Rural Chinese Population显示文摘 | Xingang Zhang Zhaoqing Sun Liqiang Zheng Jue Li Shuangshuang Liu Changlu Xu Jiajin Li Fenfen Zhao Dayi Hu Yingxian Sun | 2007 | Archives of Medical Research2007，，4： | 1 |
| 13 | Micelle formation of long - chain imidazolium ionic liquids in aqueous solution measured by isothermal titration microcalorimet:y显示文摘 | GENG FEI I IU J1E ZHENG LIQIANG | 2010 | J Chem Eng Data2010，55，： | 1 |
| 14 | The high yield synthesis and characterization of gold nanoparticles with superior sta- bility and their catalytic activity 显示文摘 | Jia Han Gao Xinpei Zheng Liqiang | 2012 | Cryst Eng Comm2012，14，22： | 1 |
| 15 | Lyotropic liquid crystalline phases formed in ternary mixtures of 1-cetyl-3-methylimidazolium bromide/p-xylene/water:a SAXS,POM,and rheology study显示文摘 | ZHANG Jin DONG Bin ZHENG Liqiang | | 0，，： | 1 |
| 16 | Surface adsorption and mieelle formation of surface active ionic liquids in aqueous solution显示文摘 | Dong Bin Li Na Zheng Liqiang | | 0，，08： | 1 |
| 17 | Dispersion of carbon nanotubes by carbazole-tailed amphiphilic imidazolium ionic liquids in aqueous solutions显示文摘 | Bin Dong Yijin Su Yonghui Liu Jie Yuan Jingkun Xu Liqiang Zheng | 2011 | Journal of Colloid And Interface Science2011，，1： | 1 |
| 18 | Dispersion of multiwalled carbon nanotubes by ionic liquid-type Gemini imidazolium surfactants in aqueous solution显示文摘 | Yonghui Liu Li Yu Shaohua Zhang Jie Yuan Lijuan Shi Liqiang Zheng | 2010 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2010，，1： | 1 |
| 19 | Fabrication of sillca nanopacticles and hollow spheres using ionic liquid microemulsion droplets as templates显示文摘 | Zhao Mingwei Zheng Liqiang Bai Xiangtao | 2009 | Colloids and Surface A:Physicochemical and Engineering Aspect2009，346，： | 1 |
| 20 | Incidenceand Predictors of Hypertension Among Chinese Adults : Results FromLiaoning Province 显示文摘 | Rural Zhaoqing Sun Liqiang Zheng Robert Detrano | 2010 | Annals of Family Medicine2010，8，1： | 1 |
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