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| 1 | Studies on in vitro regeneration competence of pseudobulb cultures in Changnienia amoena Chien显示文摘An efficient procedure is outlined for rapid and mass propagation through in vitro culture of pseudobulbs collected in different seasons of an endangered orchid, Changnienia amoena Chien. Axillary buds formed on intact pseudobulbs (collected in April) after a 12-week incubation on half-strength Murashige and Skoog (1/2 MS) medium supplemented with 1 mg L-1 N6-benzyl- adenine (6-BA), 0.5 mg L-1 α-naphthaleneacetic acid (NAA), 100 ml L-1 coconut water and 0.8 g L-1 polyvinylpyrrolidone; no buds were observed on segmentalized pseudobulbs incubated on the same medium. The axillary buds obtained from pseudobulbs growing in the natural habitat in June were detached and incubated for 7 weeks on the same medium leading to 1.4 shoot buds per explant. With repeated subculturing of the shoots on 1/2 MS medium supplemented with 2 mg L-1 6-BA and 0.5 mg L-1 NAA, a mean of 3.3 shoot buds per explant were observed on successive shoot cultures. A mean of 4.5 roots per shoot were induced on the optimal root induction medium with 1/2 MS medium plus 1.0 mg L-1 NAA and 0.1 mg L-1 6-BA and the highest rooting per centage was 88.9%. Plantlets 4-5 cm in height were transplanted into pots containing a 1:1 humus and sand mixture and grown for 7 weeks in a greenhouse before being transferred to the field. The survival rate of these transplants was about 75% after two months of growth in the wild. | JIANG WeiMei ZHAO MingShui FU ChengXin | 2011 | Chinese Science Bulletin2011，56，24： | 8 |
| 2 | Growth, photosynthesis and podophyllotoxin accumulation of Dysosma versipellis in response to a light gradient and conservation implications显示文摘Dysosma versipellis (Hance) M. Cheng ex Ying (Berberidaceae) is a rare and vulnerable, perennial herb endemic to China with pharmaceutical significance. Increasing habitat loss and over-exploitation of the plant has severely affected the plant's in situ conservation, necessitating ex situ conservation and commercial cultivation. The light regime is a critical environmental factor contributing to successful ex situ conservation via efficient production of biomass and secondary metabolites. We investigated the responses of 2-year-old D. versipellis plants to a light gradient in terms of leaf morphology, growth, biomass production, photo- synthesis and podophyllotoxin (PTOX) accumulation. D. versipellis responded to the light gradient in terms of plant height, leaf size, leaf photosynthesis and PTOX accumulation. Plants demonstrated optimal vegetative growth, photosynthetic capability, and PTOX production, under a light intensity equal to 30% full sunlight. Half full sunlight led to the lowest production of PTOX despite having no obviously different effects on growth. Full sunlight was able to cause leaf death in mid-summer (July), although these plantlets did not significantly differ in growth or biomass production before July and new leaves emerged in August. D. versipellis is therefore proposed to be a shade-tolerant plant, well adapted to variations in irradiance. Irradiance of 10%-50%, particularly ca. 30% full sunlight is strongly recommended for ex situ conservation and commercial cultivation. | ZHAO YunPeng GONG HuaDong LU WeiQiang JIANG WeiMei ZHOU XiaoLong FU ChengXin | 2011 | Chinese Science Bulletin2011，56，24： | 6 |
| 3 | An Investigation of the Potential Malignant Characteristics of Endometriosis显示文摘 | Chengxin Wang Hongyan Zhou Xiaonan You Xiji Shu Yan Li Xiaoling Zhao | 2005 | Chinese Journal of Clinical Oncology2005，2，6： | 4 |
| 4 | Two New C19-Diterpenoid Alkaloids with Anti-inflammatory Activity from Aconitum iochanicum显示文摘二新 C 19-diterpenoid 碱, 7,8-epoxy-franchetine (1 ) 和 N (19 )-en-austroconitine A (2 ) ,从 Aconitum iochanicum 被孤立。化合物 1 是有一个 7,8 环氧基树脂单位的新 C 19-diterpenoid 碱。他们的结构被包括的全面分光镜的分析阐明紫外,红外, MS, 1D 和 2D NMR。生物活动测试显示二新混合物对氮的氧化物展出了禁止的活动(没有) 在激活 LPS 的 RAW264.7 巨噬细胞的生产。与积极控制相比,二新混合物分别地与 27.3% 和 29.2% 的抑制率显示出弱反煽动性的效果。 | Ruihua Guo Chengxin Guo Dan He Dake Zhao Yong Shen | 2017 | Chinese Journal of Chemistry2017，35，10： | 3 |
| 5 | PITPNC1 promotes the thermogenesis of brown adipose tissue under acute cold exposure显示文摘Brown adipose tissue (BAT) plays an essential role in non-shivering thermogenesis. The phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) is identified as a lipid transporter that reciprocally transfers phospholipids between intracellular membrane structures. However, the physiological significance of PITPNC1 and its regulatory mechanism remain unclear. Here, we demonstrate that PITPNC1 is a key player in thermogenesis of BAT. While Pitpnc1^(−/−) mice do not differ with wildtype mice in body weight and insulin sensitivity on either chow or high-fat diet, they develop hypothermia when subjected to acute cold exposure at 4℃. The Pitpnc1^(−/−) brown adipocytes exhibit defective β-oxidation and abnormal thermogenesis-related metabolism pathways in mitochondria. The deficiency of lipid mobilization in Pitpnc1^(−/−) brown adipocytes might be the result of excessive accumulation of phosphatidylcholine and a reduction of phosphatidic acid. Our findings have uncovered significant roles of PITPNC1 in mitochondrial phospholipid homeostasis and BAT thermogenesis. | Guoqing Tang Chengxin Ma Liangkui Li Shaoyan Zhang Fengsheng Li Jin Wu Yesheng Yin Qing Zhu Yan Liang Ru Wang He Huang Tong-Jin Zhao Hongyuan Yang Peng Li Feng-Jung Chen | 2022 | Science China(Life Sciences)2022，65，11： | 2 |
| 6 | A Highly Active Pd(Ⅱ) Complex with 1-Tritylimidazole Ligand for Suzuki-Miyaura and Heck Coupling Reactions显示文摘 | Chengxin Liu Guiyan Liu Hongkun Zhao | 2016 | Chinese Journal of Chemistry2016，34，10： | 2 |
| 7 | A Bifunctional-Modulated Conformal Li/Mn-Rich Layered Cathode for Fast-Charging,High Volumetric Density and Durable Li-Ion Full Cells显示文摘Lithium-and manganese-rich(LMR)layered cathode materials hold the great promise in designing the next-generation high energy density lithium ion batteries.However,due to the severe surface phase transformation and structure collapse,stabilizing LMR to suppress capacity fade has been a critical challenge.Here,a bifunctional strategy that integrates the advantages of surface modification and structural design is proposed to address the above issues.A model compound Li_(1.2)Mn_(0.54)Ni_(0.13)Co_(0.13)O_(2)(MNC)with semi-hollow microsphere structure is synthesized,of which the surface is modified by surface-treated layer and graphene/car-bon nanotube dual layers.The unique structure design enabled high tap density(2.1 g cm^(−3))and bidirectional ion diffusion pathways.The dual surface coatings covalent bonded with MNC via C-O-M linkage greatly improves charge transfer efficiency and mitigates electrode degradation.Owing to the synergistic effect,the obtained MNC cathode is highly conformal with durable structure integrity,exhibiting high volumetric energy density(2234 Wh L^(−1))and predominant capacitive behavior.The assembled full cell,with nanograph-ite as the anode,reveals an energy density of 526.5 Wh kg^(−1),good rate performance(70.3%retention at 20 C)and long cycle life(1000 cycles).The strategy presented in this work may shed light on designing other high-performance energy devices. | Zedong Zhao Minqiang Sun Tianqi Wu Jiajia Zhang Peng Wang Long Zhang Chongyang Yang Chengxin Peng Hongbin Lu | 2021 | Nano-Micro Letters2021，13，8： | 2 |
| 8 | One-pot hydrothermal fabrication of BiVO_(4)/Fe_(3)O_(4)/rGO composite photocatalyst for the simulated solar light-driven degradation of Rhodamine B显示文摘Fabrication of easily recyclable photocatalyst with excellent photocatalytic activity for degradation of organic pollutants in wastewater is highly desirable for practical application.In this study,a novel ternary magnetic photocatalyst BiVO_(4)/Fe_(3)O_(4)/reduced graphene oxide(BiVO_(4)/Fe_(3)O_(4)/rGO)was synthesized via a facile hydrothermal strategy.The BiVO_(4)/Fe_(3)O_(4) with 0.5 wt%of rGO(BiVO_(4)/Fe_(3)O_(4)/0.5%rGO)exhibited superior activity,degrading greater than 99%Rhodamine B(RhB)after 120 min solar light radiation.The surface morphology and chemical composition of BiVO_(4)/Fe_(3)O_(4)/rGO were studied by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy,UV–visible diffuse reflectance spectroscopy,Fourier transform infrared spectroscopy,and Raman spectroscopy.The free radicals scavenging experiments demonstrated that hole(h^(+))and superoxide radical(O_(2)•^(−))were the dominant species for RhB degradation over BiVO_(4)/Fe_(3)O_(4)/rGO under solar light.The reusability of this composite catalyst was also investigated after five successive runs under an external magnetic field.The BiVO_(4)/Fe_(3)O_(4)/rGO composite was easily separated,and the recycled catalyst retained high photocatalytic activity.This study demonstrates that catalyst BiVO_(4)/Fe_(3)O_(4)/rGO possessed high dye removal efficiency in water treatment with excellent recyclability from water after use.The current study provides a possibility for more practical and sustainable photocatalytic process. | Shuangyang Zhao Chengxin Chen Jie Ding Shanshan Yang Yani Zang Nanqi Ren | 2022 | Frontiers of Environmental Science & Engineering2022，16，3： | 1 |
| 9 | Plant-protein-enabled biodegradable triboelectric nanogenerator for sustainable agriculture显示文摘As the use of triboelectric nanogenerators(TENGs)increases,the generation of related electronic waste has been a major challenge.Therefore,the development of environmentally friendly,biodegradable,and low-cost TENGs must be prioritized.Having discovered that plant proteins,by-products of grain processing,possess excellent triboelectric properties,we explore these properties by evaluating the protein structure.The proteins are recycled to fabricate triboelectric layers,and the triboelectric series according to electrical properties is determined for the first time.Using a special structure design,we construct a plant-protein-enabled biodegradable TENG by integrating a polylactic acid film,which is used as a new type of mulch film to construct a growth-promoting system that generates space electric fields for agriculture.Thus,from the plant protein to the crop,a sustainable recycling loop is implemented.Using bean seedlings as a model to confirm the feasibility of the mulch film,we further use it in the cultivation of greenhouse vegetables.Experimental results demonstrate the applicability of the proposed plant-protein-enabled biodegradable TENG in sustainable agriculture. | Chengmei Jiang Qi Zhang Chengxin He Chi Zhang Xiaohui Feng Xunjia Li Qiang Zhao Yibin Ying Jianfeng Ping | 2022 | Fundamental Research2022，2，6： | 1 |
| 10 | Behavior analysis and design of FeMnSi alloy pipe-joints显示文摘 | Lin Chengxin Gu Nanju Zhao Liancheng | 2002 | Mater Sci Forum2002，，： | 1 |
| 11 | Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver显示文摘Even though insulin-like growth factor 2(IGF2)has been reported to be overexpressed in nonalcoholic fatty liver disease(NAFLD),its role in the progression of NAFLD and the potential mechanism remain largely unclear.Using in vitro models,we found that IGF2 was the key overexpressed gene in steatosis,suggesting a possible association between IGF2 and NAFLD.Interestingly,loss-of-function experiments revealed that inhibition of IGF2 protein impaired mitochondrial biogenesis and respiration.It additionally disrupted the expression changes of mitochondrial fusion and fission-related proteins necessary in maintaining mitochondrial homeostasis.Consistently,IGF2 knockdown reduced the mitochondrial membrane potential and increased the production of reactive oxygen species.Mechanistically,IGF2 regulates mitochondrial functions by modulating the expression of SIRT1 and its downstream gene PGCla.This research opens a new frontier on the role of IGF2 in energy metabolism,which potentially participates in the development of NAFLD.As such,IGF2 is a potential therapeutic target against NAFLD. | Weiwei Gui Yiyi Zhu Shuiya Sun Weifen Zhu Bowen Tan Hanxin Zhao Chengxin Shang Fenping Zheng Xihua Lin Hong Li | 2021 | Journal of Molecular Cell Biology2021，13，8： | 1 |
| 12 | A Study on Conjunctional Cohesion Errors by CSL Learners显示文摘 | Chengxin Zhao | 2017 | US-China Education Review(A)2017，7，6： | 0 |
| 13 | Synthesis of (Ph3P)2PdX2 Complexes and Their Catalytic Activity for Heck Reaction Involving Aryl Halide and Acrylate显示文摘 | LIU Chengxin HAN Fangwai LIU Guiyan ZHAO Hongkun | 2017 | Chemical Research in Chinese Universities2017，33，6： | 0 |
| 14 | Analysis of differentially expressed genes in Verruca vulgaris vs.adjacent normal skin by RNA-sequencing显示文摘Introduction:Verruca vulgaris is one of the most common low-risk HPV infections and is characterized by excessive proliferation of keratinocytes.Currently,very little genetic information is available regarding verruca vulgaris in the Chinese population.This study aimed to obtain comprehensive transcript information of verruca vulgaris by RNA sequencing.Methods:High-throughput sequencing was performed on three fresh verruca vulgaris samples and adjacent normal skin on the Illumina sequencing platform.The transcriptomes were analyzed using bioinformatics and the differentially expressed genes(DEGs)were verified by immunohistochemistry.Verruca vulgaris exhibited a unique molecular signature.Results:In total,1,643 DEGs were identified in verruca vulgaris compared to normal skin.The functions of the DEGs were studies by Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,DEGs Reactome analysis,disease annotation function,and STRING protein-protein interaction(PPI)network analysis.The results revealed 595 GO terms associated with the cell cycle,signal transduction,immune system,signaling molecules,and interaction.The Reactome analysis revealed enrichment in reversible hydration of carbon dioxide and BMP signaling,while the disease annotation function revealed that the enriched DEGs are involved in keratosis disorders.The STRING PPI network showed that the edges with the highest density mainly included the 2′-5′oligoadenylate synthase(OAS)family-related proteins.Furthermore,the M-code analysis found ISG15,IRF7,and OASL were scored as significant modules and their high expression compared to the control was verified by immunohistochemistry.Conclusion:These findings contribute to the genetic information of verruca vulgaris in the Chinese population,revealing that interferon-stimulated genes may play essential roles in verruca vulgaris. | QINGQING GUO JIAYUE QI XIAOQIANG LIANG ZIGANG ZHAO JIA BAI FANG XIE CHENGXIN LI | 2023 | BIOCELL2023，47，11： | 0 |
| 15 | Berberine enhances the anti-hepatocellular carcinoma effect of NK92-MI cells through inhibiting IFN-gamma-mediated PD-L1 expression显示文摘Background and aims:Berberine is one of the most promising clinically tested natural alkaloids,and immunotherapy using natural killer(NK)cells is a potentially effective treatment for hepatocellular carcinoma(HCC).This study aims to elucidate the effect of berberine on the anti-HCC effect of NK92-MI cells.Materials and methods:Human HCC SMMC-7721 and Hep3B cells were co-incubated with NK92-MI cells,berberine(60 or 80 mmol/L),or their combination for 36 h.To evaluate the killing effect of NK92-MI cells on HCC cells,the release of lactate dehydrogenase(LDH)in HCC cells was measured.The anti-tumor effects of berberine,NK92-MI cells,and their combinations were evaluated by MTS,EdU,Tunel,and Western blot assays.A male BALB/c nude mouse subcutaneous tumor model was used to investigate the anti-HCC effect of berberine and NK92-MI cells in vivo.Results:The LDH assay showed that berberine enhanced the cytotoxicity of NK92-MI cells on HCC cells.The combination of berberine and NK92-MI cells demonstrated more obvious anti-HCC effect than did the berberine or NK92-MI single treatment in inhibiting cell proliferation and inducing apoptosis both in vitro and in vivo.Mechanistically,the expression of programmed cell death-ligand 1(PD-L1)in HCC cells was upregulated after co-culture with NK-92MI cells.PD-L1 expression was knocked down,thereby inhibiting the proliferation and promoting apoptosis of HCC cells,and inhibited by berberine that blocked the secretion of interferon gamma(IFN-g),thereby enhancing the anti-tumor effect of NK92-MI cells.Conclusions:Current data show that the immunomodulatory role of berberine is to enhance the cytotoxic effect of NK92-MI cells and inhibit tumor immune escape by reducing the expression of PD-L1.Our study provides a rationale for the clinical application of berberine in combination with NK cells for the treatment of HCC. | Kunyuan Wang Chengxin Gu Ganxiang Yu Jiaen Lin Zhilei Wang Qianting Lu Yangzhi Xu Dan Zhao Xiaofeng Jiang Weijian Mai Shiming Liu Hui Yang | 2022 | Liver Research2022，6，3： | 0 |
| 16 | CMOS Pixel Sensor Research and Design Progress of the SLIMP显示文摘The Silicon Lab.at IMPCAS(SLIMP)from Quark Matter Research Center(QMRC)focuses on CMOS pixel sensor design and applications.In year of 2019,we have made the following major progresses. | Zhao Chengxin Wang Yao Zhou Wei Zhang Yuezhao Li Xianqin Niu Xiaoyang Zhang Honglin Tian Yuan Gao Liling Yang Haibo | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 17 | Progress on Readout Electronics of SLIMP显示文摘The Common Data Acquisition Unit(CDAU)At present,the readout system of particle physics experiments are mainly developed on commercial systems in China.These commercial systems are not specifically designed for particle physics experiments. | Yang Haibo Li Xianqin Zhang Honglin Zhao Chengxin | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 18 | Plant and fungal species interactions differ between aboveground and belowground habitats in mountain forests of eastern China显示文摘Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways. | Teng Yang Leho Tedersoo Pamela S.Soltis Douglas E.Soltis Miao Sun Yuying Ma Yingying Ni Xu Liu Xiao Fu Yu Shi Han-Yang Lin Yun-Peng Zhao Chengxin Fu Chuan-Chao Dai Jack A.Gilbert Haiyan Chu | 2023 | Science China(Life Sciences)2023，66，5： | 0 |
| 19 | Genome-wide investigation of defensin genes in peanut(Arachis hypogaea L.)reveals AhDef2.2 conferring resistance to bacterial wilt显示文摘Peanut(Arachis hypogea L.)bacterial wilt(BW)is a devastating disease caused by Ralstonia solanacearum that results in severe yield and quality losses.Plant defensins are short cysteine-rich peptides with antimicrobial activity.The role of defensin genes(Ah Def)in peanut is unclear.A genome-wide investigation of Ah Def genes was undertaken,and 12 identified Ah Def genes were classified into two groups containing the gamma-thionin domain formed by four disulfide pairs:Cys1-Cys8,Cys2-Cys5,Cys3-Cys6,and Cys4-Cys7.In silico analysis revealed that Ah Def genes showed highly conserved architectural features and contained cis-elements associated with phytohormone signaling and defense responses.A highly resistant cultivar,H108(R)and a susceptible accession,H107(S)were tested by R.solanacearum inoculation.H108(R)showed fewer symptoms than H107(S)owing to inhibition of bacterial reproduction and spread in the vascular bundles of roots and stems.In a transcriptomic expression profile,Ah Def genes,particularly Ah Def1.6 and Ah Def2.2,were up-regulated in H108(R)compared with H107(S)under R.solanacearum infection and phytohormone treatment.Subcellular localization showed that the Ah Def1.6 and Ah Def2.2 proteins were both expressed specifically on the plasma membrane.Overexpression of protein fusion Ah Def2.2-YFP in Nicotiana benthamiana and peanut leaves increased resistance to R.solanacearum,suggesting its role in response to BW infection.Ah Def2.2 may be valuable for peanut resistance breeding. | Kai Zhao Rui Ren Xingli Ma Kunkun Zhao Chengxin Qu Di Cao Qian Ma Yingying Ma Fangping Gong Zhongfeng Li Xingguo Zhang Dongmei Yin | 2022 | The Crop Journal2022，10，3： | 0 |
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