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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Disentangling the effects of topography and space on the distributions of dominant species in a subtropical forest显示文摘Topography and space are two important factors determining plant species assemblages in forest communities.Quantification of the contribution of these two factors in determining species distribution helps us to evaluate their relative importance in determining species assemblages.This study aims to disentangle the effect of topography and space on the distributions of 14 dominant species in a subtropical mixed forest.Spearman correlation analysis and the torustranslation test were used to test the species–habitat associations.Variation partitioning was used to quantify the relative contributions of topography and space at three sampling scales and three life stages.Correlation analyses and torus-translation tests showed species abundance was mostly correlated with topographic wetness index,vertical distance from the channel network and convexity.Variation partitioning showed that pure topography,pure space and spatially structured topography explained about 2.1 %,41.2 % and 13.8 %of the variation in species distributions,respectively.For nine species,total topography fractions peaked in 20 m quadrats.For ten species,the pure space fractions peaked in 50 m quadrats.For many species,the total topography fraction andthe pure space fraction were larger for the most abundant life stages,which reflected the importance of sampling effect.However,some cases did not follow this trend suggesting that the effects of ecological processes such as habitat filtering,density dependence or dispersal limitation may exceed the sampling effects.In conclusion,we found that spatially structured topography and pure space primarily shaped the distribution of dominant tree species.Furthermore,their effects were both scale- and life stage-dependent. | Qinggang Wang Yaozhan Xu Zhijun Lu Dachuan Bao Yili Guo Junmeng Lu Kuihan Zhang Haibo Liu Hongjie Meng Xiujuan Qiao Handong Huang Mingxi Jiang | 2014 | Chinese Science Bulletin2014，59，35： | 16 |
| 3 | Establishment and preliminery use of hepatitis Bvirus preS1/2 antigen assay显示文摘 | CHEN Kun, HAN Bao Guang, MA Xian Kai, ZHANG He Qiu, MENG Li, WANG Guo Hua, XIA Fang, SONG Xiao Guo and LING Shi Gan | 1999 | World Journal of Gastroenterology1999，5，6： | 14 |
| 4 | Inner wrinkling control in hydrodynamic deep drawing of an irregular surface part using drawbeads显示文摘Inner wrinkling phenomenon is more likely to develop during hydrodynamic deep drawing(HDD) of complicated component-forms due to the higher demand for controlling deformation sequences. Aiming at the problems in control of inner wrinkling for an irregular surface part featured with both concavity and convex, this research proposes an optimal design method of drawbead parameters to change the material flow. According to theoretical analysis of the mechanism of inner wrinkling, optimizing cavity pressure only is unreasonable to form a wrinkle-free deep-drawn part,so semi-circular drawbeads are employed. The effects of layout and height of drawbeads on forming results are discussed, and a process window is established based on evaluation indicators including the anti-wrinkle coefficient and the minimum wall thickness. Experiments are carried out to validate the process window, and the wall thickness and the wrinkle height are measured and compared with numerical findings. The results show that the anti-wrinkle ability of drawbeads weakens with increasing oblique angle and distance from the die center, while the wall thickness increases with increasing oblique angle and distance, and the inner wrinkling can be completely suppressed by drawbeads arranged in zones I and II with optimum penetration. | Meng Bao Wan Min Wu Xiangdong Yuan Sheng Xu Xudong Liu Jie | 2014 | Chinese Journal of Aeronautics2014，27，3： | 14 |
| 5 | Sodium tanshinone IIA sulfonate attenuates cardiac dysfunction and improves survival of rats with cecal ligation and puncture-induced sepsis显示文摘Cardiac dysfunction, a common consequence of sepsis, is the major contribution to morbidity and mortality in patients. Sodium tanshinone IIA sulfonate(STS) is a water-soluble derivative of Tanshinone IIA(TA), a main active component of Salvia miltiorrhiza Bunge, which has been widely used in China for the treatment of cardiovascular and cerebral system diseases. In the present study, the effect of STS on sepsis-induced cardiac dysfunction was investigated and its effect on survival rate of rats with sepsis was also evaluated. STS treatment could significantly decrease the serum levels of C-reactive protein(CRP), procalcitonin(PCT), cardiac troponin Ⅰ(cTn-Ⅰ), cardiac troponin T(cTn-T), and brain natriuretic peptide(BNP) in cecal ligation and puncture(CLP)-induced) septic rats and improve left ventricular function, particularly at 48 and 72 h after CLP. As the pathogenesis of septic myocardial dysfunction is attributable to dysregulated systemic inflammatory responses, several key cytokines, including tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-10(IL-10) and high mobility group protein B1(HMGB1), were detected to reveal the possible mechanism of attenuation of septic myocardial dysfunction after being treated by STS. Our study showed that STS, especially at a high dose(15 mg×kg–1), could efficiently suppress inflammatory responses in myocardium and reduce myocardial necrosis through markedly reducing production of myocardial TNF-α, IL-6 and HMGB1. STS significantly improved the 18-day survival rate of rats with sepsis from 0% to 30%(P < 0.05). Therefore, STS could suppress inflammatory responses and improve left ventricular function in rats with sepsis, suggesting that it may be developed for the treatment of sepsis. | MENG Zheng-Jie WANG Chao MENG Ling-Tong BAO Bei-Hua WU Jin-Hui HU Yi-Qiao | 2018 | Chinese Journal of Natural Medicines2018，16，11： | 12 |
| 6 | Effect of steam-flaking on chemical compositions,starch gelatinization,in vitro fermentability,and energetic values of maize,wheat and rice显示文摘Intact and steam-flaked grains of maize,wheat and rice(with whole hulls) were analyzed for chemical composition,starch gelatinization degree(SGD) and in vitro fermentation characteristics to investigate the influence of cereal type and steam-flaking(SF) processing on their nutritive values.The treatments were arranged in a 3×2 factorial design.Obvious differences(P<0.001) in chemical composition and energetic values were observed among the different cereal types.SGD and gas production(GP) rate was significantly increased(P<0.001) by SF processing.SF processing also increased(P<0.01) the proportion of propionic acid and decreased the acetic:propionic acid ratio in vitro.Steam-flaking also increased organic matter digestibility and the energetic value of the cereal grains,especially rice.Based on these results,rice probably is more amendable to SF processing than maize and wheat.In conclusion,it is feasible to partially substitute maize grain with wheat or rice in ruminant diets,and steam-flaking can significantly improve the nutritional value of wheat and rice grains. | QIAO Fu-qiang WANG Fei REN Li-ping ZHOU Zhen-ming MENG Qing-xiang BAO Yu-hong | 2015 | Journal of Integrative Agriculture2015，14，5： | 11 |
| 7 | Assimilating Doppler radar observations with an ensemble Kalman filter for convection-permitting prediction of convective development in a heavy rainfall event during the pre-summer rainy season of South China显示文摘This study examines the effectiveness of an ensemble Kalman filter based on the weather research and forecasting model to assimilate Doppler-radar radial-velocity observations for convection-permitting prediction of convection evolution in a high-impact heavy-rainfall event over coastal areas of South China during the pre-summer rainy season. An ensemble of 40 deterministic forecast experiments(40 DADF) with data assimilation(DA) is conducted, in which the DA starts at the same time but lasts for different time spans(up to 2 h) and with different time intervals of 6, 12, 24, and 30 min. The reference experiment is conducted without DA(NODA).To show more clearly the impact of radar DA on mesoscale convective system(MCS)forecasts, two sets of 60-member ensemble experiments(NODA EF and exp37 EF) are performed using the same 60-member perturbed-ensemble initial fields but with the radar DA being conducted every 6 min in the exp37 EF experiments from 0200 to0400 BST. It is found that the DA experiments generally improve the convection prediction. The 40 DADF experiments can forecast a heavy-rain-producing MCS over land and an MCS over the ocean with high probability, despite slight displacement errors. The exp37 EF improves the probability forecast of inland and offshore MCSs more than does NODA EF. Compared with the experiments using the longer DA time intervals, assimilating the radial-velocity observations at 6-min intervals tends to produce better forecasts. The experiment with the longest DA time span and shortest time interval shows the best performance.However, a shorter DA time interval(e.g., 12 min) or a longer DA time span does not always help. The experiment with the shortest DA time interval and maximum DA window shows the best performance, as it corrects errors in the simulated convection evolution over both the inland and offshore areas. An improved representation of the initial state leads to dynamic and thermodynamic conditions that are more conducive to earlier initiation of the inland MCS and longer maintenance of the offshore MCS. | BAO XingHua LUO YaLi SUN JiaXiang MENG ZhiYong YUE Jian | 2017 | Science China Earth Sciences2017，60，10： | 11 |
| 8 | Micromanufacturing technologies of compact heat exchangers for hypersonic precooled airbreathing propulsion: A review显示文摘The Hypersonic Precooled Combined Cycle Engine(HPCCE), which introduces precooler into traditional hypersonic engine, is regarded as the most promising propulsion system for realizing a single-stage-to-orbit vehicle. The unique demands lead to the application of the compact heat exchangers, which can realize high thrust-to-weight ratio, sufficient specific impulse and high compression ratio. However, it is challenging to accurately manufacture the compact heat exchanger due to its extremely high heat dissipation capacity, remarkable compactness, superior adaptability and harsh operating condition. This review summarizes the precooling schemes of combined cycle propulsions and describes the demands and key issues in the fabrication of a compact heat exchanger for HPCCE. The investigation focuses on the application of various micromanufacturing methods of heat exchangers constructed from tubes of less than 1 mm in diameter and microchannels of less than 200 micrometers. Various micromanufacturing processes, which include microforming, micromachining, stereolithography, chemical etching, 3 D printing, joining and other advanced microfabricating processes, were reviewed. In addition, the technologies are compared in terms of dimensional tolerance, material compatibility, and process applicability. Furthermore, the boundaries of the micromanufacturing constraints are specified as references for the design of compact heat exchangers. Ultimately, the technological difficulties and development trends are discussed for the fabrication of compact heat exchangers for HPCCE. | Bao MENG Min WAN Rui ZHAO Zhengping ZOU Huoxing LIU | 2021 | Chinese Journal of Aeronautics2021，34，2： | 10 |
| 9 | Enhanced aromatic selectivity by the sheet-like ZSM-5 in syngas conversion显示文摘Aromatics are important basic chemicals. However, direct conversion of syngas via the conventional Fischer-Tropsch synthesis produces little aromatics. We presented herein that a bifunctional composite of ZSM-5 in combination with Zn Cr Oxcatalyzes syngas conversion to aromatics. Particularly, ZSM-5 crystals with a sheet-like morphology can enhance significantly the aromatization activity. The lower length ratio of the b/a axes of the crystals, the more aromatics form but without influencing the selectivity of small molecules such as CH4 and C2–C4. Since the acid properties and the Al chemical environment were not altered while the morphology changed, the enhanced aromatic selectivity is likely attributed to the favored diffusion of aromatics in these sheet-like crystals. | Junhao Yang Ke Gong Dengyun Miao Feng Jiao Xiulian Pan Xiangju Meng Fengshou Xiao Xinhe Bao | 2019 | Journal of Energy Chemistry2019，28，8： | 10 |
| 10 | Wheat genomic study for genetic improvement of traits in China显示文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world. | Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong | 2022 | Science China(Life Sciences)2022，65，9： | 9 |
| 11 | Progress in ceramic materials and structure design toward advanced thermal barrier coatings显示文摘Thermal barrier coatings(TBCs)can effectively protect the alloy substrate of hot components in aeroengines or land-based gas turbines by the thermal insulation and corrosion/erosion resistance of the ceramic top coat.However,the continuous pursuit of a higher operating temperature leads to degradation,delamination,and premature failure of the top coat.Both new ceramic materials and new coating structures must be developed to meet the demand for future advanced TBC systems.In this paper,the latest progress of some new ceramic materials is first reviewed.Then,a comprehensive spalling mechanism of the ceramic top coat is summarized to understand the dependence of lifetime on various factors such as oxidation scale growth,ceramic sintering,erosion,and calcium–magnesium–aluminium–silicate(CMAS)molten salt corrosion.Finally,new structural design methods for high-performance TBCs are discussed from the perspectives of lamellar,columnar,and nanostructure inclusions.The latest developments of ceramic top coat will be presented in terms of material selection,structural design,and failure mechanism,and the comprehensive guidance will be provided for the development of next-generation advanced TBCs with higher temperature resistance,better thermal insulation,and longer lifetime. | Zhi-Yuan WEI Guo-Hui MENG Lin CHEN Guang-Rong LI Mei-Jun LIU Wei-Xu ZHANG Li-Na ZHAO Qiang ZHANG Xiao-Dong ZHANG Chun-Lei WAN Zhi-Xue QU Lin CHEN Jing FENG Ling LIU Hui DONG Ze-Bin BAO Xiao-Feng ZHAO Xiao-Feng ZHANG Lei GUO Liang WANG Bo CHENG Wei-Wei ZHANG Peng-Yun XU Guan-Jun YANG Hong-Neng CAI Hong CUI You WANG Fu-Xing YE Zhuang MA Wei PAN Min LIU Ke-Song ZHOU Chang-Jiu LI | 2022 | Journal of Advanced Ceramics2022，11，7： | 9 |
| 12 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 13 | Three-dimensional interconnected Ni(Fe)OxHy nanosheets on stainless steel mesh as a robust integrated oxygen evolution electrode显示文摘 | Qi Zhang Haixia Zhong Fanlu Meng Di Bao Xinbo Zhang Xiaolin wei | 2018 | Nano Research2018，11，3： | 8 |
| 14 | Inhibition of growth and metastasis of triple-negative breast cancer targeted by Traditional Chinese Medicine Tubeimu in orthotopic mice models显示文摘Objective: Triple-negative breast cancer(TNBC) is highly invasive and metastatic, which is in urgent need of transformative therapeutics. Tubeimu(TBM), the rhizome of Bolbostemma paniculatum(Maxim.) Franquet, is one of the Chinese medicinal herbs used for breast diseases since the ancient times. The present study evaluated the efficacy, especially the anti-metastatic effects of the dichloromethane extract of Tubeimu(ETBM) on TNBC orthotopic mouse models and cell lines.Methods: We applied real-time imaging on florescent orthotopic TNBC mice model and tested cell migration and invasion abilities with MDA-MB-231 cell line. Digital gene expression sequencing was performed and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis applied to explore the pathways influenced by ETBM.Moreover, quantitative real-time polymerase chain reactions(q RT-PCR) and Western blot were delivered to confirm the gene expression changes.Results: ETBM exhibited noticeable control on tumor metastasis and growth of TNBC tumors with no obvious toxicity. In compliance with this, it also showed inhibition of cell migration and invasion in vitro. Its impact on the changed biological behavior in TNBC may be a result of decreased expression of integrin β1(ITGβ1), integrin β8(ITGβ8) and Rho GTPase activating protein 5(ARHGAP5), which disabled the focal adhesion pathway and caused change in cell morphology.Conclusions: This study reveals that ETBM has anti-metastatic effects on MDA-MB-231-GFP tumor and may lead to a new therapeutic agent for the integrative treatment of highly invasive TNBC. | Jingxiao Wang Xinjie Yang Haibo Han Limin Wang Weiqian Bao Shanshan Wang Robert M.Hoffman Meng Yang Hui Qi Chao An Kaiwen Hu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 8 |
| 15 | Design,methodology,and baseline of whole city‑million scale children and adolescents myopia survey(CAMS)in Wenzhou,China显示文摘Background:Myopia is the most common visual impairment in children and adolescents worldwide.This study described an economical and effective population-based screening pipeline and performed the project of a million scale children and adolescents myopia survey(CAMS),which will shed light on the further study of myopia from the level of epidemiology and precision medicine.Methods:We developed a novel population-based screening pattern,an intelligent screening process and internetbased information transmission and analysis system to carry out the survey consisting of school children in Wenzhou,China.The examination items include unaided distance visual acuity,presenting distance visual acuity,and noncycloplegic autorefraction.Myopia and high myopia were defined as spherical equivalent(SE)≤−1.00 diopters(D)and SE≤−6.00 D,respectively.Next,the reports of the vision checking were automatically sent to parents and the related departments.The CAMS project will be done two to four times annually with the support of the government.An online eyesight status information management system(OESIMS)was developed to construct comprehensive and efficient electronic vision health records(EVHRs)for myopia information inquiry,risk pre-warning,and further study.Results:The CAMS completed the first-round of screening within 30 days for 99.41%of Wenzhou students from districts and counties,in June 2019.A total of 1,060,925 participants were eligible for CAMS and 1,054,251(99.37%participation rate)were selected through data quality control,which comprised 1305 schools,and 580,609,251,050 and 170,967 elementary,middle,and high school students.The mean age of participants was 12.21±3.32 years(6-20 years),the female-to-male ratio was 0.82.The prevalence of myopia in elementary,middle,and high school students was 38.16%,77.52%,and 84.00%,respectively,and the high myopia incidence was 0.95%,6.90%,and 12.98%.Conclusions:The CAMS standardized myopia screening model involves automating large-scale information collection,data transmission,data analysis and early warning,thereby supporting myopia prevention and control.The entire survey reduced 90%of staff,cost,and time consumption compared with previous surveys.This will provide new insights for decision support for public health intervention. | Liangde Xu Youyuan Zhuang Guosi Zhang Yunlong Ma Jian Yuan Changseng Tu MiaoMiao Li Wencan Wang Yaru Zhang Xiaoyan Lu Jing Li Xinting Liu Zhengbo Xue Meng Zhou Jie Sun Jinhua Bao Ming Li Fan Lu Hong Wang Jianzhong Su Jia Qu | 2021 | Eye and Vision2021，8，1： | 7 |
| 16 | A new unconventional HLA-A2-restricted epitope from HBV core protein elicits antiviral cytotoxic T lymphocytes显示文摘细胞毒素的 T 房间(CTL ) 在肝炎 B 的控制起一个关键作用病毒(HBV ) 感染和病毒的清理。然而,大多数识别 CTL epitopes 从遗传型 A 和 D 的 HBV 被导出,并且很少在在亚洲更流行的遗传型 B 和 C 的病毒被定义。因为 HBV 核心蛋白质(HBc ) 是最保守、产生免疫性的部件,在这研究,我们使用了盖住 HBc 屏蔽并且识别特定的 CTL epitopes 的一个重叠 9-mer 肽水池。异乎寻常的 HLA-A2-restricted epitope HBc141149 被结晶化分析发现,在结构上描绘了。immunogenicity 和 anti-HBV 活动进一步在 HBV 和 HLA-A2 被决定转基因的老鼠。最后,我们证明在 HBc141149 epitope 的变化与病毒的参数被联系,在 HBV 的疾病前进感染了病人。我们的数据因此提供卓见进这异乎寻常的 epitope 绑定的结构特征给 MHC-I 分子,以及 epitope 特定的 CTL 活动在 HBV 安排 T 房间反应和有免疫力的避免感染的病人。 | Lu Sun Yu Zhang Bao Zhao Mengmeng Deng Jun Liu Xin Li Junwei Hou Mingming Gui Shuijun Zhang Xiaodong Li George F. Gao Songdong Meng | 2014 | Protein & Cell2014，5，4： | 7 |
| 17 | Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses显示文摘Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.3.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.3.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.3.4.4 b HA gene, an H5 N6 virus bearing the clade 2.3.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.3.4.4 h or 2.3.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature. | ZENG Xian-ying HE Xin-wen MENG Fei MA Qi WANG Yan BAO Hong-mei LIU Yan-jing DENG Guo-hua SHI Jian-zhong LI Yan-bing TIAN Guo-bin CHEN Hua-lan | 2022 | Journal of Integrative Agriculture2022，21，7： | 7 |
| 18 | Competitive virus and host RNAs: the interplay of a hidden virus and host interaction显示文摘在病毒感染期间,病毒的 RNA 和 mRNAs 在天生的免疫作为联系病原体的分子的模式(PAMP ) 为病毒的蛋白质合成并且可能作为蓝图工作。这里,在 microRNAs (miRNAs ) 和竞争内长的 RNA (ceRNAs ) 考虑最近的研究进步,我们作为海绵推测那病毒的 RNA 行为并且能扣押在内的内长的 miRNAs 感染了细胞,这样跨 regulating 稳定性和有分享的 miRNA 反应元素的宿主 mRNAs 的翻译效率。在病毒的 RNA 和主人 mRNAs 之间的这串音和这些相互的相互作用被称为竞争病毒并且主人 RNA (cvhRNAs ) 。我们进一步在肝炎 B 为 cvhRNAs 网络的存在提供最近的试验性的证据病毒(HBV ) ,以及 Herpesvirus saimiri (HVS ) , lysin 的鼠科的 cytomegalovirus (MCMV ) 和人的 cytomegalovirus (HCMV ) 感染。另外, cvhRNA 假设也预言可能在主人和另外的病毒之间跨规定例如丙肝病毒(HCV ) , HIV,流行性感冒病毒,人的乳头状瘤病毒(HPV ) 。因为在 miRNAs 和病毒的 RNA 之间的相互作用不可避免地也导致病毒的 RNA 功能的压抑,我们推测那个病毒可以演变采用 cvhRNA 网络或避免在主机以内为最佳的健康指向的 miRNA。CvhRNA 网络可以因此在病毒的复制,感染建立,和病毒的致病的规定起一个基本作用。 | Changfei Li Jun Hu Junli Hao Bao Zhao Bo Wu Lu Sun Shanxin Peng George F. Gao Songdong Meng | 2014 | Protein & Cell2014，5，5： | 6 |
| 19 | Mutation Analysis of AVPR2 and AQP2 Gene in Chinese Patients with Congenital Nephrogenic Diabetes Insipidus显示文摘To detect mutations of the aquaporin 2 gene(AQP2) and the arginine vasopressin V2 receptor gene(AVPR2) of Chinese congenital nephrogenic diabetes insipidus, and to establish the foundation for further studying the emergence mechanism of the disease and clinical diagnosis, all the exons and part of introns of AQP2 and AVPR2 genes were amplified with intronic primers, using genomic DNA extracted from three patients with congenital nephrogenic diabetes insipidus and two mothers as template, PCR product was ligated into a T-vector and then sequenced. The result was compared with the database sequence to identify the mutable sites via a BLAST search, the incidence of every mutation was analyzed, and the putative transcription factor binding sites that maybe disturbed were analyzed by MAPPER. Mutation g.1394A>G in exon 3 of AVPR2 was detected in all the subjects, g.861C>T(S167L) in exon 2 of AVPR2 and IVS1+3G>A in intron of AQP2 were detected, respectively, in two patients, and c.836A>C in 3′ untranslated region of AQP2 was detected in two patients and one mother. Four mutations were identified. g.1394A>G of AVPR2 and c.836A>C of AQP2 have high incidence in patients with nephrogenic diabetes insipidus. Detection on the two sites may become auxiliary diagnosis index of congenital nephrogenic diabetes insipidus. | WANG Ying LI Hong-jun YU Zhen-xiang BAO Yong-li WU Yin YU Chun-lei MENG Xiang-ying LI Yu-xin | 2008 | Chemical Research in Chinese Universities2008，24，3： | 6 |
| 20 | Selective conversion of syngas to propane over ZnCrO_x-SSZ-39 OX-ZEO catalysts显示文摘Oxide-Zeolite(OX-ZEO) bifunctional catalyst design concept has been exemplified in several processes to direct conversion syngas to value-added chemicals and fuels such as mixed light olefins, ethylene, aromatics and gasoline.Herein we demonstrate that the product can be steered toward liquefied petroleum gas(LPG) with a selectivity up to 89% in hydrocarbons especially propane selectivity reaching 80% at CO conversion of 63% using ZnCrOx-H-SSZ-39 catalyst.Interestingly, the quantity of the acid sites of SSZ-39 does not influence obviously the hydrocarbon distribution but the strength is crucial for selective formation of propane.This finding provides an alternative route of LPG synthesis from a variety of carbon resources via syngas. | Gen Li Feng Jiao Dengyun Miao Yong Wang Xiulian Pan Toshiyuki Yokoi Xiangju Meng Feng-Shou Xiao Andrei-Nicolae Parvulescu Ulrich Müller Xinhe Bao | 2019 | Journal of Energy Chemistry2019，28，9： | 6 |
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