|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 2 | Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus显示文摘 | Cuiqing Zhong Meiling Zhang Qi Yin Han Zhao Yang Wang Sexin Huang Wenrong Tao Keliang Wu Zi-Jiang Chen Jinsong Li | 2016 | Cell Research2016，26，6： | 16 |
| 3 | Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection显示文摘 | YuxuanWu Li Yang Jinsong Li Cuiqing Zhong Zhenfei Xie Qi Yin Rui Dong Suming Yang | 2016 | Cell Research2016，26，1： | 13 |
| 4 | Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy显示文摘 | Keliang Wu Cuiqing Zhong Tailai Chen Xiaoyu Zhang Wenrong Tao Jingye Zhang Hongchang Li Han Zhao Jinsong Li Zi-Jiang Chen | 2017 | Cell Research2017，27，8： | 13 |
| 5 | Preparation of Unsupported Molybdenum Phosphide Hydrofining Catalysts显示文摘 | LI Fengyan, ZHAO Tianbo, SUN Guida, LI Cuiqing (Department of Applied Chemistry, Beijing Institute of Petrochemical Technology, Beijing 102617, China) | 2003 | 催化学报2003，24，2： | 10 |
| 6 | Mitochondrial replacement by pre-pronuclear transfer in human embryos显示文摘 | Wu, Keliang Chen, Tailai Huang, Sexin Zhong, Cuiqing Yan, Junhao Zhang, Xiaoyu Li, Jinsong Gao, Yuan Zhao, Han Chen, Zi-Jiang | 2017 | Cell Research2017，27，6： | 7 |
| 7 | Dosage effect of multiple genes accounts for multisystem disorder of myotonic dystrophy type 1显示文摘Multisystem manifestations in myotonic dystrophy type 1(DM1)may be due to dosage reduction in multiple genes induced by aberrant expansion of CTG repeats in DMPK,including DMPK,its neighboring genes(SIX5 or DMWD)and downstream MBNLh However,direct evidence is lacking.Here,we develop a new strategy to generate mice carrying multigene heterozygous mutations to mimic dosage reduction in one step by injection of haploid embryonic stem cells with mutant Dmpk,Six5 and Mbnll into oocytes.The triple heterozygous mutant mice exhibit adult-onset DM1 phenotypes.With the additional mutation in Dmwd,the quadruple heterozygous mutant mice recapitulate many major manifestations in congenital DM1.Moreover,muscle stem cells in both models display reduced sternness,providing a unique model for screening small molecules for treatment of DM1.Our results suggest that the complex symptoms of DM1 result from the reduced dosage of multiple genes. | Qi Yin Hongye Wang Na Li Yifu Ding Zhenfei Xie Lifang Jin Yan Li Qiong Wang Xinyi Liu Liuqing Xu Qing Li Yongjian Ma Yanbo Cheng Kai Wang Cuiqing Zhong Qian Yu Wei Tang Wanjin Chen Wenjun Yang Fan Zhang Chen Ding Lan Bao Bin Zhou Ping Hu Jinsong Li | 2020 | Cell Research2020，30，2： | 7 |
| 8 | Similar Ability of FbaA with M Protein to Elicit Protective Immunity Against Group A Streptococcus Challenge in Mice显示文摘Group A streptococcus(GAS),an important human pathogen,can cause various kinds of infections including superficial infections and potentially lethal infections,and the search for an effective vaccine to prevent GAS infections has been ongoing for many years. This paper compares the immunogenicity and immunoprotection of FbaA(an Fn-binding protein expressed on the surface of GAS) with that of M protein,the best immunogen of GAS. Assay for immune response showed that FbaA,similar to M protein,could induce protein-specific high IgG titer in BALB/c mice. Furthermore,following GAS challenge,the mice immunized with FbaA showed the same protective rate as those with M protein. These results indicate that FbaA is similar in ability to M protein in inducing protective immunity against GAS challenge in mice. | Cuiqing Ma Caihong Li Xiurong Wang Ruihong Zeng Xiaolin Yin Huidong Feng Lin Wei | 2009 | Cellular & Molecular Immunology2009，6，1： | 4 |
| 9 | Preparation of microbial desulfurization catalysts显示文摘A Rhodococcus sp. lawq, a bacterium isolated from the soil cleaving the C-S bond of dibenzothiophene (DBT) via specific pathway, was investigated for cell growth and for its role in desulfurization. Clearly, the end product, 2-hydroxybiphenyl, inhibited the growth of the strain, the synthesis of the desulfurization enzymes, and the activity of the enzymes. The effects of sulfate on the DBT degradation enzymes were examined in the Rhodococcus sp. lawq growth system with DBT; the sulfate served, concurrently, as the sulfur source. The condition of the resting cells that were used in desulfurization, was also studied. The optimal concentration of the resting cells and the reaction conditions were determined. It was documented that there is no difference between desulfurization activity for resting cells cultured with sulfate as the sole sulfur source and that with the mixture of DBT and sulfate as the sulfur source. | XU Ping MA Cuiqing LI Fuli TONG Mingyou ZENG Yiyong WANG Shuning H.D.Blankespoor | 2002 | Chinese Science Bulletin2002，47，13： | 4 |
| 10 | Medium optimization by combination of response surface methodology and desirability function: an application in glutamine production显示文摘 | Jinshan Li Cuiqing Ma Yanhe Ma Yan Li Wei Zhou Ping Xu | 2007 | Applied Microbiology and Biotechnology2007，，3： | 1 |
| 11 | Medium Optimization by Combination of Response Surface Methodology and Desirability Function :an Application in Glutamine Production显示文摘 | Jinshan Li Cuiqing Ma Yanhe Ma | 2007 | Appl Micrabiol Biotechnol2007，74，： | 1 |
| 12 | Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM显示文摘 | Cuiqing Ma Ailong Wang Jiayang Qin Lixiang Li Xulu Ai Tianyi Jiang Hongzhi Tang Ping Xu | 2009 | Applied Microbiology and Biotechnology2009，，1： | 1 |
| 13 | Gefitinib-sensitive mutations of the epidermalgrowth factor receptor tyrosine kinase domain in Chi-nese patients with non-small cell lung cancer显示文摘 | Mu X L Li L Y Zhang X T’Wang M Z5Feng R E’CuiQ C | 2005 | ClinCancer Res2005，11，： | 1 |
| 14 | Effect of Reduction Temperature on Hydrofining Performance of Supported Molybdenum Phosphide Catalyst显示文摘A series of supported molybdenum phosphide catalysts were prepared by impregnation method. XRD, TG-DTG, XPS and BET were used to study the phase, compositions and surface areas of the prepared catalysts. A model reactant containing thiophene, pyridine and cyclohexene was used for the measurements of catalytic activities. The effect of reduction temperature on catalytic activities was in- vestigated. The analysis results by XRD and BET are very different when the reduction temperature is changed from 400 to 900 °C. MOP/γ-Al2 O3 catalysts and CoMoP/γ-Al2O3 catalysts prepared at the reduction temperature of 500 °C are the most active ones. | Fengyan Li Zhifang Zhao Qingjie Li Tianbo Zhao Cuiqing Li Guida Sun | 2005 | Journal of Natural Gas Chemistry2005，14，4： | 0 |
| 15 | Joint Effects of Heat Stress and PM_(2.5) Exposure on Glucose Metabolism and Hepatic Insulin Signaling显示文摘Background:Heatwave events occur more frequently,accompanied by a significant increase in the ambient concentration of fine particulate matter(PM 2.5).Epidemiological and experimental studies have suggested that heat stress or PM 2.5 exposure would impair glucose homeostasis and insulin sensitivity.Objective:To explore the joint effects of heat stress and PM 2.5 exposure on glucose metabolism and hepatic insulin pathway.Methods:C57BL/6 mice were randomly divided into filtered air(FA)group,fine particulate matter(PM)group,filtered air combined with heat stress(FH)group,and PM combined with heat stress(PH)group.Mice were exposed to PM for a total of 6 weeks,and heat stress combined with PM exposure was applied at the 5th and 6th weeks via a whole-body exposure system.Systemic glucose homeostasis,insulin sensitivity,and circulating inflammatory cytokines were examined.HSP72 expression and insulin signaling in the liver were measured.Results:Glucose tolerance and insulin sensitivity were impaired in response to heat stress,accompanied by less-ened hepatic GLUT2 expression and inhibited insulin signaling pathway.No synergistic effects of heat stress and PM 2.5 exposure on glucose homeostasis were observed,while heat-upregulated HSP72 expression was attenuated with accumulated TNF��induced by further PM 2.5 exposure.Conclusion:Heat stress combined with PM 2.5 exposure induced TNF��,which could inhibit heat-elevated hepatic HSP72 expression.Elevated circulating TNF��impaired hepatic insulin signaling and GLUT2 expression.Then,glucose homeostasis was perturbed,and insulin action was impaired. | Weijia Gu Ziwei Cai Ruiqing Wang Mianhua Zhong Lung-Chi Chen Lu Zhang Rucheng Chen Ran Li Li Qin Qinghua Sun Cuiqing Liu | 2023 | Clinical Complementary Medicine and Pharmacology2023，3，2： | 0 |
| 16 | Synthesis, characterization and hydrotreating performance of supported tungsten phosphide catalysts显示文摘Supported tungsten phosphide catalysts were prepared by temperature-programmed reduction of their precursors(supported phospho-tungstate catalysts)in H2 and characterized by X-ray diffraction(XRD),BET,temperature-programmed desorption of ammonia(NH3-TPD)and X-ray photoelectron spectroscopy(XPS).The reduction-phosphiding processes of the precursors were investigated by thermogravimetry and differential thermal analysis(TG-DTA)and the suitable phosphiding temperatures were defined.The hydrodesulfurization(HDS)and hydrodenitrogenation(HDN)activities of the catalysts were tested by using thiophene,pyridine,dibenzothiophene,carbazole and diesel oil as the feedstock.The TiO2,c-Al_(2)O_(3) supports and the Ni,Co promoters could remarkably increase and stabilize active W species on the catalyst surface.A suitable amount of Ni(3%–5%),Co(5%–7%)and V(1%–3%)could increase dispersivity of the W species and the BET surface area of the WP/c-Al_(2)O_(3) catalyst.The WP/c-Al_(2)O_(3) catalyst possesses much higher thiophene HDS and carbazole HDN activities and the WP/TiO2 catalyst has much higher dibenzothiophene(DBT)HDS and pyridine HDN activities.The Ni,Co and V can obviously promote the HDS activity and inhibit the HDN activity of the WP/c-Al_(2)O_(3) catalyst.The G-Ni5 catalyst possesses a much higher diesel oil HDS activity than the sulphided industrial NiW/c-Al_(2)O_(3) catalyst.In general,a support or promoter in the WP/c-Al_(2)O_(3) catalyst which can increase the amount and dispersivity of the active W species can promote its HDS and HDN activities. | Guida SUN Cuiqing LI Zhijun ZHOU Fengyan LI | 2008 | Frontiers of Chemical Science and Engineering2008，2，2： | 0 |
| 17 | Towards capturing of totipotency显示文摘In June issue of Cell Research,Xu et al.demonstrate that totipotent-like stem cells(TPS cells)can be derived directly from a 2-cell mouse embryo and extended pluripotent stem(EPS)cells by a chemical cocktail(CPEC condition).In addition,the study shows the advanced potential of TPS cells in lineage differentiation and blastocyst-like structure generation,and provides insight into mechanistic regulation of totipotency.Totipotency,the ability of a single cell to generate all the differentiated cells in an organism,is present in the mouse zygote as well as in the 2-cell blastomeres.How to capture the totipotent state in a dish has been a challenge for many decades.Recently,studies reported the successful conversion of pluripotent stem cells(PSCs)into totipotent-like cells by spliceosomal repression1 or modulation of chromatin-remodeling enzymes.2 However,whether the totipotency can be better captured and maintained in culture directly from 2-cell embryos remains less well characterized. | Cuiqing Zhong Ronghui Li Juan Carlos Izpisua Belmonte | 2022 | Cell Research2022，32，8： | 0 |
| 18 | Transcriptomic profiling fuels the derivation of stable pig epiblast stem cells显示文摘It is controversial whether pig pluripotent stem cells can be derived and stably maintained in a culture dish.In a recent paper published in Cell Research,Zhi et al.perform a comprehensive transcriptome landscape analysis of pig embryos at all developmental stages from embryonic(E) day 0 to E14 and identify a culture cocktail to stabilize pig pregastrulation epiblast stem cells(pgEpiSCs)in vitro for long-term passaging;the pgEpiSCs can undergo multiple rounds of gene editing and generate cloned animals. | Cuiqing Zhong Ronghui Li Juan Carlos lzpisua Belmonte | 2022 | Cell Research2022，32，4： | 0 |
| 19 | Many-objective Optimization Method Based on Dimension Reduction for Operation of Large-scale Cooling Energy Systems显示文摘Large-scale cooling energy system has developed well in the past decade.However,its optimization is still a problem to be tackled due to the nonlinearity and large scale of existing systems.Reducing the scale of problems without oversimplifying the actual system model is a big challenge nowadays.This paper proposes a dimension reduction-based many-objective optimization(DRMO)method to solve an accurate nonlinear model of a practical large-scale cooling energy system.In the first stage,many-objective and many-variable of the large system are pre-processed to reduce the overall scale of the optimization problem.The relationships between many objectives are analyzed to find a few representative objectives.Key control variables are extracted to reduce the dimension of variables and the number of equality constraints.In the second stage,the manyobjective group search optimization(GSO)method is used to solve the low-dimensional nonlinear model,and a Pareto-front is obtained.In the final stage,candidate solutions along the Paretofront are graded on many-objective levels of system operators.The candidate solution with the highest average utility value is selected as the best running mode.Simulations are carried out on a 619-node-614-branch cooling system,and results show the ability of the proposed method in solving large-scale system operation problems. | Peng Zhu Lixiao Wang Cuiqing Wu Jinyu Yu Zhigang Li Jiehui Zheng Qing-Hua Wu | 2023 | CSEE Journal of Power and Energy Systems2023，9，3： | 0 |
| 20 | Analysis of the Implementation of Integrated Healthcare Management in Preoperative Preparation显示文摘Objective:To strengthen preoperative preparation management and reduce preoperative preparation defects.Methods:We set up a special medical care management team to investigate the preoperative preparation in surgical departments,analyze the causes of the shortcomings in preoperative preparation,and propose countermeasures.The medical staff jointly formulated plans related to preoperative preparation management including clear outline of responsibilities,strengthening of training,integrated medical care,timely feedback,and continuous improvement.Besides,the preoperative preparation of patients before and after the improvement measures were compared using statistical analysis.Results:The rate of inadequate preoperative preparation was 11.14 per thousand before the integrated management model was implemented.After the implementation of the integrated management model,the rate of inadequate preoperative preparation decreased to 2.99 per thousand,and the difference was statistically(P<0.05).Conclusion:The integrated management of preoperative preparation can help reduce the rate of preoperative complications and ensure the safety of surgery. | Huiling Liu Xuling Li Xinyi Liu Yuhua Ma Haomin Li Cuiqing Liu | 2023 | Journal of Clinical and Nursing Research2023，7，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员