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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Efficacy and safety of a novel anti-HER2 therapeutic antibody RC48 in patients with HER2-overexpressing,locally advanced or metastatic gastric or gastroesophageal junction cancer:a single-arm phase II study显示文摘Background:Current treatment options for human epidermal growth factor receptor 2(HER2)-overexpressing gastric cancer at third-line have shown limited clinical benefit.Further,there is no specific treatment for HER2 immunohistochemistry(IHC)2+and fluorescence in-situ hybridization-negative patients.Here,we report the efficacy and safety of a novel anti-HER2 antibody RC48 for patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer.Methods:Patients with HER2-overexpressing(IHC 2+or 3+),locally advanced or metastatic gastric or gastroesophageal junction cancer who were under at least second-line therapy were eligible and received RC482.5 mg/kg alone every 2 weeks.The primary endpoint was the objective response rate(ORR)assessed by an independent review committee.Secondary endpoints included progressionfree survival(PFS),overall survival(OS),duration of response,time to progression,disease control rate,and safety.Results:Of 179 patients screened,125 were eligible and received RC48 treatment.The ORR was 24.8%(95%confidence interval[CI]:17.5%-33.3%).The median PFS and OS were 4.1 months(95%CI:3.7-4.9 months)and 7.9 months(95%CI:6.7-9.9 months),respectively.The most frequently reported adverse events were decreased white blood cell count(53.6%),asthenia(53.6%),hair loss(53.6%),decreased neutrophil count(52.0%),anemia(49.6%),and increased aspartate aminotransferase level(43.2%).Serious adverse events(SAEs)occurred in 45(36.0%)patients,and RC48-related SAEs were mainly decreased neutrophil count(3.2%).Seven patients had adverse events that led to death were not RC48-related.Conclusions:RC48 showed promising activity with manageable safety,suggesting potential application in patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer who have previously received at least two lines of chemotherapy. | Zhi Peng Tianshu Liu Jia Wei Airong Wang Yifu He Liuzhong Yang Xizhi Zhang Nanfeng Fan Suxia Luo Zhen Li Kangsheng Gu Jianwei Lu Jianming Xu Qingxia Fan Ruihua Xu Liangming Zhang Enxiao Li Yuping Sun Guohua Yu Chunmei Bai Yong Liu Jiangzheng Zeng Jieer Ying Xinjun Liang Nong Xu Chao Gao Yongqian Shu Dong Ma Guanghai Dai Shengmian Li Ting Deng Yuehong Cui Jianmin Fang Yi Ba Lin Shen | 2021 | Cancer Communications2021，41，11： | 29 |
| 3 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 4 | Clinical efficacy of 0.1% pranoprofen in treatment of dry eye patients:a multicenter, randomized, controlled clinical trial显示文摘 | Chen Jingyao Dong Fei Chen Wei Sun Xuguang Deng Yingping Hong Jing Zhang Mingchang Yang Wenzhao Liu Zuguo Xie Lixin | 2014 | Chinese Medical Journal2014，，13： | 18 |
| 5 | An Integrated Analysis of Risk Factors of Cognitive Impairment in Patients with Severe Carotid Artery Stenosis显示文摘Objective To investigate cognitive dysfunction in patients with carotid artery stenosis(CAS) and potential risk factors related to cognitive-especially memory-dysfunction. Methods Forty-seven patients with carotid artery stenosis were recruited into our study cohort. The Mini-Mental State Examination(MMSE) and the Montreal Cognitive Assessment(MoCA) were adopted to assess cognitive function, the Wechsler Memory Scale(WMS) to assess memory function, high-resolution MRI and enhanced ultrasound to evaluate carotid plaques, and computed tomography perfusion(CTP) imaging to evaluate intracranial blood perfusion. Single-factor analysis and multiple-factor regression analysis were used to analyze potential risk factors of cognitive impairment. Results Mini-Mental State Examination test results showed that 22 patients had cognitive impairment, and MoCA test results showed that 10 patients had cognitive impairment. Analysis of various risk factors indicated that the average memory quotient of female patients was higher than that of males(P = 0.024). The cognitive and memory performance of those with an educational background above high school were significantly better than those of participants with high school or lower(P = 0.045). Patients with abnormal intracranial perfusion performed worse on the MMSE test(P = 0.024), and their WMS scores were significantly lower(P = 0.007). The MMSE scores and the memory quotients were significantly lower in patients with a history of cerebral infarction(MMSE, P = 0.047, memory quotient score, P = 0.018). Conclusion A history of cerebral infarction and abnormal cerebral perfusion are associated with decline in overall cognitive function and memory in patients with carotid stenosis. Being female and having an educational background above high school may be protective factors in the development of cognitive dysfunction. | LUO Ru Tao WANG Pei Jiong DENG Xiao Feng ZHOU Shu Jie ZHAO Meng QIAN Jing ZHANG Dong WANG Rong ZHANG Yan | 2018 | Biomedical and Environmental Sciences2018，31，11： | 17 |
| 6 | Enriched environment boosts the post-stroke recovery of neurological function by promoting autophagy显示文摘Autophagy is crucial for maintaining cellular homeostasis,and can be activated after ischemic stroke.It also participates in nerve injury and repair.The purpose of this study was to investigate whether an enriched environment has neuroprotective effects through affecting autophagy.A Sprague-Dawley rat model of transient ischemic stroke was prepared by occlusion of the middle cerebral artery followed by reperfusion.One week after surgery,these rats were raised in either a standard environment or an enriched environment for 4 successive weeks.The enriched environment increased Beclin-1 expression and the LC3-II/LC3-I ratio in the autophagy/lysosomal pathway in the penumbra of middle cerebral artery-occluded rats.Enriched environment-induced elevations in autophagic activity were mainly observed in neurons.Enriched environment treatment also promoted the fusion of autophagosomes with lysosomes,enhanced the lysosomal activities of lysosomal-associated membrane protein 1,cathepsin B,and cathepsin D,and reduced the expression of ubiquitin and p62.After 4 weeks of enriched environment treatment,neurological deficits and neuronal death caused by middle cerebral artery occlusion/reperfusion were significantly alleviated,and infarct volume was significantly reduced.These findings suggest that neuronal autophagy is likely the neuroprotective mechanism by which an enriched environment promotes recovery from ischemic stroke.This study was approved by the Animal Ethics Committee of the Kunming University of Science and Technology,China(approval No.5301002013855)on March 1,2019. | Yi-Hao Deng Ling-Ling Dong Yong-Jie Zhang Xiao-Ming Zhao Hong-Yun He | 2021 | Neural Regeneration Research2021，16，5： | 17 |
| 7 | Association of serum vascular endothelial growth factor-C and lymphatic vessel density with lymph node metastasis and prognosis of patients with gastric cancer显示文摘AIM: To investigate whether serum vascular endothelial growth factor-C (SVEGF-C), VEGF-C, and lymphatic vessel density (LVD) in tumor tissues are related to lymph node metastasis (LNM) and prognosis in gastric cancer.METHODS: SVEGF-C levels of 80 gastric cancer patients and 20 healthy donors were examined using ELISA. VEGF-C expression and LVD were examined using immunohistochemical staining. Kaplan-Meier survival analysis was performed to determine their influence on the prognosis of the patients. RESULTS: The SVEGF-C level in gastric cancer patients (595.9 ± 201.0 ng/L) was significantly higher (P = 0.000) than controls (360.0 ± 97.4 ng/L). Both SVEGF-C and LVD were significantly higher in poorly differentiated adenocarcinomas, T3 and T4, LNM, distant metastasis, and pTNM groups Ⅲ and IV (P = 0.000). The sensitivity and specificity of SVEGF-C for predicting LNM were 82.8% and 81.8%, respectively (cut-off = 542.5 ng/L). The positive expression rate of VEGF-C was significantly higher in cancerous than in normal tissues (65% vs 20%; P = 0.001). VEGF-C expression up-regulation was significantly related to differentiation, depth of invasion, LNM, distant metastasis, and pTNM stage (P = 0.000). LVD was 10.7 ± 3.1/200 HP in the experimental group vs 4.9 ± 1.3/200 HP in controls (P = 0.000); LVD in cancerous tissues with and without LNM was 12.0 ± 2.7/200 HP vs 7.6 ± 0.5/200 HP, respectively (P = 0.000). SVEGF-C and LVD were significantly higher in VEGF-C positive than in negative patients (P=0.000); SVEGF-C level was related to LVD (P = 0.000). Kaplan-Meier survival analysis factors predicating poor prognosis were: SVEGF-C level (P = 0.001), VEGF-C expression and LVD (both P = 0.000). CONCLUSION: SVEGF-C level, VEGF-C and LVD are related to LNM and poor prognosis of patients with gastric cancer. SVEGF-C may be a biomarker for LNM in gastric cancer. | Tian-Bao Wang Mei-hai Deng Wan-Shou Qiu Wen-Guang Dong | 2007 | World Journal of Gastroenterology2007，13，12： | 17 |
| 8 | Three-dimensional Evolutionary Models of the Qiongxi Structures,Southwestern Sichuan Basin,China:Evidence from Seismic Interpretation and Geomorphology显示文摘褶层结束是在有压缩性的差错相关的褶层的学习的特色。如此的结束能由于戳差错上的排水量的损失或 / 并且形成一个侧面或倾斜的斜面。因此,高质量的地震数据将明白地帮助定义哪个机制应该为给定的差错相关的褶层的结束负责。在四川盆的 Qiongxi 和 Qiongxinan 结构,中国是拥有在他们之间的北结束和结构的僵绳的自然差错繁殖褶层的例子。合拢 / 指责几何学和沿着罢工排水量变化被工业 3-D 抑制地震体积。我们解释北结束和结构的僵绳附近的褶层的 plunge 由于排水量的损失罢工。与北结束联系的差错几何学变化从一在 Qiongxinan 结构的冠扁平斜面在排水量最大的地方,到简单地 Qiongxi 结构的北尖端附近的一个斜面,没有形成一个侧面或倾斜的斜面。在这研究,我们也使用排水模式,在沿着一个差错的排水量与推进为 Qiongxinan 的侧面的繁殖建议一个模型的持续时间成正比的 Qiongxinan 结构和假设的冠保存的胚胎的结构和 Qiongxi 结构。明确地,我们建议结构首先在易碎的单位以内作为一个孤立的差错斜面开始了。与弄短增加,差错变得以更弱的页岩单位与更低的分队连接创造 hybridized 差错繁殖褶层。我们的模型为 Qiongxinan 和 Qiongxi 结构的侧面的繁殖历史建议可能的解释,并且也提供一条其他的途径给在迟了的新生代在西南的四川盆证实以前的 Pingluoba 结构的活动。 | JIA Qiupeng JIA Dong LUO Liang CHEN Zhuxin LI Yiquan DENG Fei SUN Shengsi LI Haibin | 2009 | Acta Geologica Sinica(English Edition)2009，83，2： | 17 |
| 9 | Zircon and apatite fission track analyses on mineralization ages and tectonic activities of Tuwu-Yandong porphyry copper deposit in northern Xinjiang, China显示文摘The mineralization ages reported in the past in the Tuwu-Yandong copper district not only are different,but also fall into the Hercynian epoch.This study has achieved 9 zircon and 7 apatite fission track analysis results.The zircon fission track ages range from 158 Ma to 289 Ma and the apatite ages are between 64 Ma and 140 Ma.The mineralization accords with the regional tectonics in the copper district.We consider that the zircon fission track age could reveal the mineralization age based on annealing zone temperature of 140―300℃ and retention temperature of ~250℃ for zircon fission track,and metallogenetic temperature of 120―350℃ in this ore district.Total three mineralization epochs have been identified,i.e.,289―276 Ma,232―200 Ma and 165―158 Ma,and indicate occurrence of the min-eralization in the Indosinian and Yanshan epochs.Corresponding to apatite fission track ages,the three tectonic-mineralizing epochs are 140―132 Ma,109―97 Ma and 64 Ma,which means age at about 100℃ after the mineralization.The three epochs lasted 146 Ma,108 Ma and about 100 Ma from ~250℃ to ~100℃ and trend decrease from early to late.It is shown by the fission track modeling that this district underwent three stages of geological thermal histories,stable in Cretaceous and cooling both before Cretaceous and after 20 Ma. | YUAN WanMing1,BAO ZengKuan2,DONG JinQuan2,GUO ZhaoJie3 & DENG Jun1 1 State Key Laboratory of Geological Processes and Mineral Resources,China University of Geosciences,Beijing100083,China 2 Laboratory of Nuclear Analysis Techniques,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100039,China 3 Department of Geology,Peking University,Beijing 100871,China | 2007 | Science China Earth Sciences2007，50，12： | 16 |
| 10 | DETERMINGING BPA UNDER UNCERTAINTY ENVIRONMENTS AND ITS APPLICATION IN DATA FUSION显示文摘Dempster-Shafer (DS) theory of evidence has been widely used in many data fusion ap- plication systems. However, how to determine basic probability assignment, which is the main and the first step in evidence theory, is still an open issue. In this paper, a new method to obtain Basic Probability Assignment (BPA) is proposed based on the similarity measure between generalized fuzzy numbers. In the proposed method, species model can be constructed by determination of the min, average and max value to construct a fuzzy number. Then, a new Radius Of Gravity (ROG) method to determine the similarity measure between generalized fuzzy numbers is used to calculate the BPA functions of each instance. Finally, the efficiency of the proposed method is illustrated by the classi- fication of Iris data. | Deng Yong Jiang Wen Xu Xiaobin Li Qi Wang Dong | 2009 | Journal of Electronics(China)2009，26，1： | 15 |
| 11 | Effects of land uses on soil physic-chemical properties and erodibility in collapsing-gully alluvial fan of Anxi County,China显示文摘As a special kind of soil erosion that severely deteriorates the qualities of soil in granite regions,collapsing gully is widely distributed in the tropical and subtropical areas of South China.In particular,collapsing-gully erosion produces a large amount of sediment deposited on the plough layer of alluvial fan farmland and causes increase of desertification,great reduction of nutrients and rapid enhancement of erodibility in the soil.This study was designed to evaluate the effects of different land uses on the soil physic-chemical properties and erodibility of collapsing-gully alluvial fan.Our results show that the physical properties of soil in alluvial fan were greatly improved with smaller bulk density,increased soil porosity,strengthened water holding capacity and a higher particulate matter content.The chemical properties of soil were also significantly improved,including pH,cation exchange capacity,and the contents of organic matter,total and available nitrogen,potassium and phosphorus.All the land uses were proved to improve the soil properties,especially in the tea garden,vegetable land and paddy field.The results of correlation analyses among the properties demonstrate that the physical and chemical properties were significantly correlated,indicating that improving the soil physical properties is an effective method to increase the soil nutrient in the farmland of collapsing-gully alluvial fan.In addition,the application of land uses included grass land,eucalyptus forest land,vegetable land,tea garden,sweet potato land,and paddy field reduced the erodibility of the soil compare to bare land.The erodibility K values of soils in grass land,eucalyptus forest land,vegetable land,tea garden,sweet potato land,and paddy field were 14.43,16.97,45.45,33.12,18.94,and 34.01%lowerthan those of bare land,respectively.The results of multiple linear regression analysis show that the soil erodibility had a significant correlation with the physic-chemical properties,especially the soil texture and the content of organic matter.In conclusion,our results demonstrate that different land use patterns are effective to improve the quality of the soil in collapsing-gully alluvial fan,including the improvements of the soil structure,nutrients and anti-erosion ability.Ourfindings provide important implications for the soil improvement in the farmland of collapsing-gully alluvial fan. | DENG Yu-song XIA Dong CAI Chong-fa DING Shu-wen | 2016 | Journal of Integrative Agriculture2016，15，8： | 15 |
| 12 | Endothelial progenitor cells in peripheral blood may serve as a biological marker to predict severe acute pancreatitis显示文摘AIM To investigate the significance of endothelial progenitor cells(EPCs) in predicting severe acute pancreatitis(SAP).METHODS We recruited 71 patients with acute pancreatitis(AP) and excluded 11 of them; finally, cases of mild acute pancreatitis(MAP)(n = 30) and SAP(n = 30), and healthy volunteers(n = 20) were internalized to investigate levels of EPCs, C-reactive protein(CRP), tumor necrosis factor-alpha(TNF-α), fibrinogen(FIB)and white blood cells(WBC) in peripheral blood. RESULTS The levels of TNF-α, WBC, FIB and CRP were higher both in SAP and MAP cases than in healthy volunteers(P < 0.05, all). Interestingly, the level of EPCs was higher in SAP than MAP(1.63% ± 1.47% vs 6.61% ± 4.28%, P < 0.01), but there was no significant difference between the MAP cases and healthy volunteers(1.63% ± 1.47% vs 0.55% ± 0.54%, P > 0.05). Receiver operating characteristics curve(ROC) showed that EPCs, TNF-α, CRP and FIB were significantly associated with SAP, especially EPCs and CRP were optimal predictive markers of SAP. When the cut-off point for EPCs and CRP were 2.26% and 5.94 mg/d L, the sensitivities were 90.0% and 73.3%, and the specificities were 83.3% and 96.7%. Although, CRP had the highest specificity, and EPCs had the highest sensitivity and highest area under the curve value(0.93). CONCLUSION Data suggest that EPCs may be a new biological marker in predicting SAP. | Xiao-Qin Ha Yue-Juan Song Hong-Bin Zhao Wei-Wei Ta Hong-Wei Gao Qiang-Sheng Feng Ju-Zi Dong Zhi-Yun Deng Hong-Yan Fan Jun-Hua Peng Zhi-Hua Yang Yong Zhao | 2017 | World Journal of Gastroenterology2017，23，14： | 15 |
| 13 | A newly discovered Gigantopithecus fauna from Sanhe Cave, Chongzuo, Guangxi, South China显示文摘Among the most important faunas in the Late Cenozoic, the Gigantopithecus faunas have received a good deal of attention. The Gigantopithecus fauna recently discovered in Sanhe Cave consists of more than 80 mammal species, including cf. Hominidae, Pongo sp., Hylobates sp., Sinomastodon yangzien-sis, Stegodon preorientalis, Cervavitus fenqii, Dicoryphochoerus ultimus and Sus xiaozhu. It is the southernmost Gigantopithecus fauna found so far in China. Its geological age is estimated to be Early Pleistocene based on the fauna and stratigraphic correlation. The significant increase in the estimated body sizes of Ailuropoda, Gigantopithecus and Tapirus shows that the Sanhe fauna is middle Early Pleistocene, later than those from Wushan and Liucheng (early Early Pleistocene). Paleomagnetic dating of the fossil-bearing strata in Sanhe Cave gives an age of approximately 1.2 Ma. The fauna is characterized by tropical-subtropical forest types, including Pongo sp., Tupaia sp., Ia sp., Typhlomys intermedius, etc., and it lacks Palaearctic types. It is a typical tropical forest fauna, suggesting an en-vironment with a lush forest and a warm and humid climate. The discovery of the Sanhe Gigantopith-ecus fauna is significant for establishing the chronological stages of the Gigantopithecus faunas in China, and for discussing their origin, evolution and dynamics. | JIN ChangZhu QIN DaGong PAN WenShi TANG ZhiLu LIU JinYi WANG Yuan DENG ChengLong ZHANG YingQi DONG Wei TONG HaoWen | 2009 | Chinese Science Bulletin2009，54，5： | 14 |
| 14 | Nitrification characteristics of nitrobacteria immobilized in waterborne polyurethane in wastewater of corn-based ethanol fuel production显示文摘A technology to achieve stable and high ammonia nitrogen removal rates for corn distillery wastewater (ethanol fuel production) treatment has been designed.The characteristics of nitrifying bacteria entrapped in a waterborne polyurethane (WPU) gel carrier were evaluated after acclimation.In the acclimation period,nitrification rates of WPU-immobilized nitrobacteria were monitored and polymerase chain reaction (PCR) was also carried out to investigate the change in ammonium-oxidizing bacteria.The results showed that the pellet nitrification rates increased from 21 to 228 mg-N/(L-pellet·hr) and the quantity of the ammonia oxidation bacteria increased substantially during the acclimation.A continuous ammonia removal experiment with the anaerobic pond effluent of a distillery wastewater system was conducted with immobilized nitrifying bacteria for 30 days using an 80 L airlift reactor with pellets at a fill ratio of 15% (V/V).Under the conditions of 75 mg/L influent ammonia,hydraulic retention time (HRT) of 3.7-5.6 hr,and dissolved oxygen (DO) of 4 mg/L,the effluent ammonia concentration was lower than 10 mg/L and the ammonia removal efficiency was 90%.While the highest ammonia removal rate,162 mg-N/(L-pellet·hr),was observed when the HRT was 1.3 hr. | Yamei Dong Zhenjia Zhang Yongwei Jin Jian Lut Xuehang Cheng Jun Li Yan-yan Deng Ya-nan Feng Dongning Chen | 2012 | Journal of Environmental Sciences2012，24，6： | 14 |
| 15 | Neuroprotective Autophagic Flux Induced by Hyperbaric Oxygen Preconditioning is Mediated by Cystatin C显示文摘We have previously reported that Cystatin C(CysC) is a pivotal mediator in the neuroprotection induced by hyperbaric oxygen(HBO) preconditioning; however,the underlying mechanism and how CysC changes after stroke are not clear. In the present study, we demonstrated that CysC expression was elevated as early as 3 h after reperfusion, and this was further enhanced by HBO preconditioning. Concurrently, LC3-II and Beclin-1, two positive-markers for autophagy induction, exhibited increases similar to CysC, while knockdown of CysC blocked these elevations. As a marker of autophagy inhibition, p62 was downregulated by HBO preconditioning and this was blocked by CysC knockdown. Besides, the beneficial effects of preserving lysosomal membrane integrity and enhancing autolysosome formation induced by HBO preconditioning were abolished in CysC-/-rats.Furthermore, we demonstrated that exogenous CysC reduced the neurological deficits and infarct volume after brain ischemic injury, while 3-methyladenine partially reversed this neuroprotection. In the present study, we showed that CysC is biochemically and morphologically essential for promoting autophagic flux, and highlighted the translational potential of HBO preconditioning and CysC for stroke treatment. | Zongping Fang Yun Feng Yuheng Li Jiao Deng Huang Nie Qianzhi Yang Shiquan Wang Hailong Dong Lize Xiong | 2019 | Neuroscience Bulletin2019，35，2： | 14 |
| 16 | Hand-sewn vs linearly stapled esophagogastric anastomosis for esophageal cancer:A meta-analysis显示文摘AIM: To compare the outcomes of hand-sewn(HS) and linearly stapled(LS) esophagogastric anastomosis for esophageal cancer.METHODS: Before beginning this study, a rigorous protocol was established according to the recommendations of the Cochrane Collaboration. Databases and references were searched for all randomizedcontrolled trials and comparative clinical studies that compared LS with HS esophagogastric anastomosis for esophageal cancer. The primary outcomes compared were anastomotic leak and stricture. Subgroup analyses were performed according to site of anastomosis.RESULTS: Fifteen studies were used, comprising 3203 patients(n = 2027 LS and 1176 HS). Primary outcome analysis revealed a significant decrease in anastomotic leakage(RR = 0.51, 95%CI: 0.41-0.65; P < 0.00001) a s s o c i a t e d w i t h L S a n a s t o m o s i s. A s i g n i f i c a n t l y reduced rate of anastomotic stricture associated with LS was also found(RR = 0.56, 95%CI: 0.49-0.64; P < 0.00001). A subgroup analysis according to the site of anastomosis revealed a significantly reduced rate of anastomotic stricture(P < 0.00001). Although there was no significant difference in the decrease in thoracic anastomotic leakage, there was a significant decrease in cervical anastomotic leakage associated with LS(P < 0.00001).CONCLUSION: This meta-analysis indicates that the LS technique contributes to a reduced rate of leakage and stricture compared with the HS method. | Xu-Feng Deng Quan-Xing Liu Dong Zhou Jia-Xin Min Ji-Gang Dai | 2015 | World Journal of Gastroenterology2015，21，15： | 13 |
| 17 | Irregular surface of carotid atherosclerotic plaque is associated with ischemic stroke: a magnetic resonance imaging study显示文摘Objective To determine the association between the irregularity of carotid plaque surface using multidimensional magnetic resonance imaging(MRI) of ipsilateral acute cerebral infarction(ACI) cases. Methods Patients with recent cerebrovascular symptoms(stroke or transient ischemic attack < 2 weeks) and atherosclerotic plaque in at least one carotid artery were diagnosed by B-mode ultrasound imaging(intima-media thickness ≥ 1.5 mm) and recruited for the present study. Irregular surface was defined when plaque surface was uneven with high and low fluctuation or plaque with surface ulceration. The irregularity of carotid plaque surface was determined on axial or oblique images alone(single-dimension) and on both axial images and oblique images(multidimensions), separately. Univariate and multivariate logistic regression analyses were performed to calculate the odds ratio(OR) and the corresponding 95% CI of the irregular plaque surface in discriminating the presence of ipsilateral ACI. Results A total of 217 included subjects(mean age: 60.7 ± 10.2 years, 149 men) were recruited and 89(41.0%), 88(40.6%) and 118(54.4%) of them exhibited irregular plaque surface on axial, oblique and multidimensional MR images, respectively. The OR of irregularity of the plaque surface was determined by multidimensional MRI to be 5.88(95% CI: 3.16–10.96, P < 0.001) in discriminating the presence of ipsilateral ACI. Following adjustment for clinical confounding factors, this association remained statistically significant(OR = 5.65, 95% CI: 2.53–12.60, P < 0.001). The analysis included further adjustment for the presence of lipid-rich necrotic core, intraplaque hemorrhage and stenosis and the results included that this association also remained statistically significant(OR = 6.08, 95% CI: 2.52–14.68, P < 0.001). Conclusions The irregular plaque surface was determined by multidimensional MRI as an independent indicator for ipsilateral acute cerebral infarction. | Dan ZHOU Jin LI Dong LIU Li-Ya JI Na-Qin WANG Jie DENG Ji-Chen WANG Min YE Xi-Hai ZHAO | 2019 | Journal of Geriatric Cardiology2019，16，12： | 13 |
| 18 | Riboflavin-induced Priming for Pathogen Defense in Arabidopsis thaliana显示文摘Riboflavin (vitamin B2) participates in a variety of redox processes that affect plant defense responses. Previously we have shown that riboflavin induces pathogen resistance in the absence of hypersensitive cell death (HCD) in plants. Herein, we report that ribo-avin induces priming of defense responses in Arabidopsis thaliana toward infection by virulent Pseudomonas syringae pv. tomato DC3000 (Pst). Induced resistance was mechanistically connected with the expression of defense response genes and cellular defense events, including H2O2 burst, HCD, and callose deposition in the plant. Riboflavin treatment and inoculation of plants with Pst were neither active but both synergized to induce defense responses. The priming process needed NPR1 (essential regulator of systemic acquired resistance) and maintenance of H2O2 burst but was independent of salicylic acid, jasmonic acid, ethylene, and abscisic acid. Our results suggest that the role of riboflavin in priming defenses is subject to a signaling process distinct from the known pathways of hormone signal transduction. | Shujian Zhang Xue Yang Maowu Sun Feng Sun Sheng Deng Hansong Dong | 2009 | Journal of Integrative Plant Biology2009，51，2： | 12 |
| 19 | Multi-factor analysis of initial poor graft function after orthotopic liver transplantation显示文摘BACKGROUND: In the early period of orthotopic liver transplantation (OLT), initial poor graft function (IPGF) is one of the complications which leads to primary graft non-function (PGNF) in serious cases. This study set out to establish the clinical risk factors resulting in IPGF after OLT. METHODS: Eighty cases of OLT were analyzed. The IPGF group consisted of patients with alanine aminotransferase (ALT) and/or aspartate aminotransferase (AST) above 1500 IU/L within 72 hours after OLT, while those in the non-IPGF group had values below 1500 IU/L. Recipient-associated factors before OLT analyzed were age, sex, primary liver disease and Child-Pugh classification; factors analyzed within the peri-operative period were non-heart beating time (NHBT), cold ischemia time (CIT), rewarming ischemic time (RWIT), liver biopsy at the end of cold ischemia; and factors analyzed within 72 hours after OLT were ALT and/or AST values. A logistic regression model was applied to filter the possible factors resulting in IPGF. RESULTS: Donor NHBT, CIT and RWIT were significantly longer in the IPGF group than in the non-IPGF group; in the logistic regression model, NHBT was the risk factor leading to IPGF (P<0.05), while CIT and RWIT were possible risk factors. In one case in the IPGF group, PGNF appeared with moderate hepatic steatosis. CONCLUSIONS: Longer NHBT is an important risk factor leading to IPGF, while serious steatosis in the donor liver, CIT and RWIT are potential risk factors. | Chen, Hao Peng, Cheng-Hong Shen, Bai-Yong Deng, Xia-Xing Shen, Chuan Xie, Jun-Jie Dong, Wei Li, Hong-Wei | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，2： | 12 |
| 20 | miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened显示文摘AIM:To investigate the regulative effect of miRNA-3383p(miR-338-3p) on cell growth in colorectal carcinoma(CRC).METHODS:The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed.The recombinant viral vector encoding the pre-miR338-3p or miR-338-3p-inhibitor and the two packaging plasmids psPAX2 and pMD2.G were cotransfected into human embryonic kidney 293T cells to package lentivirus.The supernatant containing the lentivirus particles was harvested to determine the viral titer,and this supernatant was then used to transduce CRCderived cell line,SW-620.Flow cytometry was utilized for sorting the green fluorescent protein(GFP) + cells to establish the SW-620 cell line stably expressing premiR-338-3p or miR-338-3p-inhibitor.Moreover,the expression of miR-338-3p was determined by real-time reverse transcriptase polymerase chain reaction,andWestern blotting was used to detect the expression of the smoothened(SMO,the possible target of miR-3383p) protein in SW-620 cells.Furthermore,the status of CRC cell proliferation and apoptosis were detected by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry,respectively.RESULTS:Restriction enzyme digestion and DNA sequencing demonstrated that the lentiviral vector pLVTHM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed successfully.GFP was expressed after the SW-620 cells were transduced by the lentivirus.Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p was significantly increased(relative expression 3.91 ± 0.51 vs 2.36 ± 0.44,P < 0.01).Furthermore,overexpression of miR-338-3p inhibited the expression of SMO protein in SW-620 cells,which showed obviously suppressed proliferation ability [cellular proliferation inhibition rate(CPIR) 61.9% ± 5.2% vs 41.6% ± 4.8%,P < 0.01].Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p-inhibitor was significantly decreased(relative expression 0.92 ± 0.29 vs 2.36 ± 0.44,P < 0.01).Moreover,the downregulated expression of miR-338-3p caused upregulated expression of the SMO protein in SW-620 cells,which showed significantly enhanced proliferation ability(CPIR 19.2% ± 3.8% vs 41.6% ± 4.8%,P < 0.01).However,anti-SMO-siRNA largely,but not completely,reversed the effects induced by blockage of miR-338-3p,suggesting that the regulative effect of miR-338-3p on CRC cell growth was indeed mediated by SMO.CONCLUSION:miR-338-3p could suppress CRC growth by inhibiting SMO protein expression. | Kai Sun Hai-Jun Deng Shang-Tong Lei Jing-Qing Dong Guo-Xin Li | 2013 | World Journal of Gastroenterology2013，19，14： | 12 |
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