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| 1 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 2 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
| 3 | Application of CRISPR/Cas9 in plant biology显示文摘The CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPRassociated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. | Xuan Liu Surui Wu Jiao Xu Chun Sui Jianhe Wei | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 8 |
| 4 | 为细菌的房间的一个非凡的紧、敏感的光可切换的基因表达式系统显示文摘 | Chen, Xianjun Liu, Renmei Ma, Zhengcai Xu, Xiaopei Zhang, Haoqian Xu, Jianhe Ouyang, Qi Yang, Yi | 2016 | Cell Research2016，26，7： | 7 |
| 5 | LOWER DOSE OF AMINOGLYCOSIDE OTOTOXIC EXPOSURE CAUSES PRESYNAPTIC ALTERATIONS ASSOICATED WITH HEARING LOSS显示文摘Objective To study presynaptic alternations of cochlear ribbons arising from aminoglycoside ototoxic stimuli in C57BL/6J mice. Methods Animals were injected with low dose gentamicin(100 mg/kg/day) for14 days, From the 14 th to 28 th days, the mice were maintained free of gentamicin treatment. Immunohistochemistry labeling was employed to trace RIBEYE, a major presynaptic componment of ribbon synapses.RIBEYE/CtBP2 expression levels were assessed and compared with hearing threshold shifts. Auditory function was assessed by auditory brainstem responses. The stereocilia of outer hair cells(OHCs) and IHCs was examined by scanning electron microscopy(SEM). Results Hearing thresholds were elevated with peak hearing loss observed on the 7th day after gentamicin exposure, followed by improvement after the 7th day.RIBEYE/CtBP2 expression directly correlated with observed hearing threshold shifts. Strikingly, we did not see any obvious changes in stereocilia in both OHCs and IHCs until the 28 th day. Mild changes in stereocilia were only observed in OHCs on the 28 th day. Conclusions These findings indicate that presynapse cochlear ribbons, rather than stereocilia, may be sensitive to aminoglycoside ototoxic exposure in mice cochleae. A pattern of RIBEYE/CtBP2 expression changes seems to parallel hearing threshold shifts and suggests presynaptic response properties to lower dosage of aminoglycoside ototoxic stimuli. | LIU Ke WANG Xiaoyu LI Sijun TANG Siquan XU Yice WANG Xuefeng SUN Jianhe YANG Weiyan YANG Shiming | 2014 | Journal of Otology2014，9，1： | 2 |
| 6 | Chinese tomato yelIow Ieaf curl virus— a new species of geminivirus显示文摘 | Yule Liu Jianhe Cai Dongling Li Bixia Qin Bo Tian | 1998 | Science in China Series C: Life Sciences1998，，4： | 1 |
| 7 | Percutaneous nephroscopic with holmium laser and ultrasound lithotripsy for complicated renal calculi显示文摘 | Zhengqin Gu Jun Qi Haibo Shen Jianhe Liu Jianhua Chen | 2010 | Lasers in Medical Science2010，，4： | 1 |
| 8 | Chinese tomato yelIow Ieaf curl virus— a new species of geminivirus显示文摘 | Yule Liu Jianhe Cai Dongling Li Bixia Qin Bo Tian | 1998 | Science in China Series C: Life Sciences1998，，4： | 1 |
| 9 | Metformin suppresses calcium oxalate crystal-induced kidney injury by promoting Sirt1 and M2 macrophage-mediated anti-inflammatory activation显示文摘Dear Editor,Kidney stones are one of the most common clinical diseases in urology.Approximately 80%of renal calculi are composed of calcium oxalate(CaOx)crystals,which are gradually deposited in the medullary collecting duct or in the renal interstitium,causing nephrocalcinosis.1 Although CaOx nephrocalcinosis is usually asymptomatic. | Haoran Liu Chen Duan Xiaoqi Yang Jianhe Liu Yaoliang Deng Hans-Göran Tiselius Zhangqun Ye Tao Wang Jinchun Xing Hua Xu | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 1 |
| 10 | Chinese tomato yelIow Ieaf curl virus— a new species of geminivirus显示文摘 | Yule Liu Jianhe Cai Dongling Li Bixia Qin Bo Tian | 1998 | Science in China Series C: Life Sciences1998，，4： | 1 |
| 11 | Chinese tomato yelIow Ieaf curl virus— a new species of geminivirus显示文摘 | Yule Liu Jianhe Cai Dongling Li Bixia Qin Bo Tian | 1998 | Science in China Series C: Life Sciences1998，，4： | 1 |
| 12 | Discussing the Dietary Regimen and Disease Prevention and its Application as an Anti‑epidemic Measure显示文摘This article discusses the role of dietary regimen in the traditional culture of Chinese medicine according to its origin.Dietary regimen is a technique that inherits the traditional culture of Chinese medicine.Through analysis of the characteristics of the dietary regimen and the discussion of the idea of disease prevention,the application of the dietary regimen in disease prevention is sought.Under the guidance of three key factors,the application of the dietary regimen in disease prevention is mainly manifested in two aspects.First,the dietary regimen can be used to guide the daily reasonable diet to maintain the human body in a balance of Yin and Yang,which could prevent the occurrence of diseases.Second,on the basis of minimizing health loss,it can be used for disease treatment,adjuvant treatment,and rehabilitation.Finally,taking preventive treatment as the guiding ideology,the application of a dietary regimen as an“anti‑epidemic”was briefly discussed. | Jianhe Fang Weifeng Zhu Hongning Liu | 2020 | Chinese Medicine and Culture2020，3，3： | 1 |
| 13 | Chinese tomato yelIow Ieaf curl virus— a new species of geminivirus显示文摘 | Yule Liu Jianhe Cai Dongling Li Bixia Qin Bo Tian | 1998 | Science in China Series C: Life Sciences1998，，4： | 1 |
| 14 | Assessing the mechanism of citywide test-trace-isolate Zero-COVID policy and exit strategy of COVID-19 pandemic显示文摘Background:Countries that aimed for eliminating the cases of COVID-19 with test-trace-isolate policy are found to have lower infections,deaths,and better economic performance,compared with those that opted for other mitigation strategies.However,the continuous evolution of new strains has raised the question of whether COVID-19 eradication is still possible given the limited public health response capacity and fatigue of the epidemic.We aim to investigate the mechanism of the Zero-COVID policy on outbreak containment,and to explore the possibility of eradication of Omicron transmission using the citywide test-trace-isolate(CTTI)strategy.Methods:We develop a compartmental model incorporating the CTTI Zero-COVID policy to understand how it contributes to the SARS-CoV-2 elimination.We employ our model to mimic the Delta outbreak in Fujian Province,China,from September 10 to October 9,2021,and the Omicron outbreak in Jilin Province,China for the period from March 1 to April 1,2022.Projections and sensitivity analyses were conducted using dynamical system and Latin Hypercube Sampling/Partial Rank Correlation Coefcient(PRCC).Results:Calibration results of the model estimate the Fujian Delta outbreak can end in 30(95%confdence interval CI:28–33)days,after 10(95%CI:9–11)rounds of citywide testing.The emerging Jilin Omicron outbreak may achieve zero COVID cases in 50(95%CI:41–57)days if supported with sufcient public health resources and population compliance,which shows the efectiveness of the CTTI Zero-COVID policy.Conclusions:The CTTI policy shows the capacity for the eradication of the Delta outbreaks and also the Omicron outbreaks.Nonetheless,the implementation of radical CTTI is challenging,which requires routine monitoring for early detection,adequate testing capacity,efcient contact tracing,and high isolation compliance,which constrain its benefts in regions with limited resources.Moreover,these challenges become even more acute in the face of more contagious variants with a high proportion of asymptomatic cases.Hence,in regions where CTTI is not possible,personal protection,public health control measures,and vaccination are indispensable for mitigating and exiting the COVID-19 pandemic. | Pei Yuan Yi Tan Liu Yang Elena Arufo Nicholas H.Ogden Guojing Yang Haixia Lu Zhigui Lin Weichuan Lin Wenjun Ma Meng Fan Kaifa Wang Jianhe Shen Tianmu Chen Huaiping Zhu | 2022 | Infectious Diseases of Poverty2022，11，5： | 0 |
| 15 | There is the second virus that causes tobacco leaf curl disease (not TbLCV-CHI) in the field显示文摘Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in the Guangxi Zhuang Autonomous Region, China. This virus DNA-A contains 2 734 nt. Large intergenic region (LIR) contains 269 nt, the virus sense strand contains 2 open reading frames (ORFs): AV1 (115 aa) and AV2 (coat protein gene, CP, 256 aa), and the complementary sense strand contains 4 ORFs: AC1 (replicase gene, 361 aa), AC2 (transactivator, 134 aa), ACS (134 aa) and AC4 (97 aa). The virus belongs to one kind of subgroup III gemini- viruses from old world, and could be the Chinese tomato yellow leaf curl virus (TYLCV-CHI). | GONG Qianhong LIU Yule HONG Yiguo WANG Huanyu CHEN Gang CAI Jianhe | 2000 | Chinese Science Bulletin2000，45，12： | 0 |
| 16 | A chromosome-level genome assembly for Dracaena cochinchinensis reveals the molecular basis of its longevity and formation of dragon’s blood显示文摘Dracaena,a remarkably long-lived and slowly maturing species of plant,is world famous for its ability to produce dragon’s blood,a precious traditional medicine used by different cultures since ancient times.However,there is no detailed and high-quality genome available for this species at present;thus,the molecular mechanisms that underlie its important traits are largely unknown.These factors seriously limit the protection and regeneration of this rare and endangered plant resource.Here,we sequenced and assembled the genome of Dracaena cochinchinensis at the chromosome level.The D.cochinchinensis genome covers 1.21 Gb with a scaffold N50 of 50.06 Mb and encodes 31619 predicted protein-coding genes.Analysis showed that D.cochinchinensis has undergone two whole-genome duplications and two bursts of long terminal repeat insertions.The expansion of two gene classes,cis-zeatin O-glucosyltransferase and small auxin upregulated RNA,were found to account for its longevity and slow growth.Two transcription factors(bHLH and MYB)were found to be core regulators of the flavonoid biosynthesis pathway,and reactive oxygen species were identified as the specific signaling molecules responsible for the injuryinduced formation of dragon’s blood.Our study provides high-quality genomic information relating to D.cochinchinensis and significant insight into the molecular mechanisms responsible for its longevity and formation of dragon’s blood.These findings will facilitate resource protection and sustainable utilization of Dracaena. | Yanhong Xu Kaijian Zhang Zhonglian Zhang Yang Liu Feifei Lv Peiwen Sun Shixi Gao Qiuling Wang Cuicui Yu Jiemei Jiang Chuangjun Li Meifang Song Zhihui Gao Chun Sui Haitao Li Yue Jin Xinwei Guo Jianhe Wei | 2022 | Plant Communications2022，3，6： | 0 |
| 17 | Discussing the Dietary Regimen and Disease Prevention and its Application as an Anti-epidemic Measure显示文摘This article discusses the role of dietary regimen in the traditional culture of Chinese medicine according to its origin.Dietary regimen is a technique that inherits die traditional culture of Chinese medicine.Through analysis of the characteristics of the dietary regimen and the discussion of the idea of disease prevention,the application of the dietary regimen in disease prevention is sought.Under the guidance of three key factors,the application of the dietary regimen in disease prevention is mainly manifested in two aspects,h irst,the dietary regimen can be used to guide the daily reasonable diet to maintain the human body in a balance of Yin and Yang,which could prevent the occurrence of diseases.Second,on the basis of minimizing health loss,it can be used for disease treatment,adjuvant treatment,and rehabilitation.Finally,taking preventive treatment as the guiding ideology,the application of a dietary regimen as an'anti-epidemic'was briefly discussed. | Jianhe Fang Weifeng Zhu Hongning Liu | 2019 | Chinese Medicine and Culture2019，2，1： | 0 |
| 18 | Efficacy of Jiangzhi Xiaoban tablet(降脂消斑片)on toll-like receptor 4/nuclear factor-kappa B/nod-like receptor protein 3 signaling pathway in mice with atherosclerosis induced by high-fat diet显示文摘OBJECTIVE:To study the effect of Jiangzhi Xiaoban tablet(降脂消斑片,JZXB)on toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)/Nod-like receptor protein 3(NLRP3)signaling pathway expression in atherosclerosis(AS)mice by establishing a mouse model of AS,and to explore its mechanism of prevention and treatment of AS.METHODS:Sixty-four male C57BL/6J mice were randomly divided into two groups,12 in the normal control group and 52 in the model group(MOD).Seven weeks later,two mice in each of the above two groups were randomly sacrificed,and the whole aortic tissue of the mice was taken out for hematoxylin-eosin staining.After successful modeling,50 mice in the modeling group were randomly divided into 5 groups:MOD,atorvastatin group(ATO),low-dose group of JZXB(JZXB-L),middle-dose group of JZXB(JZXB-M),and high-dose group of JZXB(JZXB-H),10 mice in each group.The mice in each group were killed after 6 weeks of preventive administration.HE staining was used to observe the pathological changes of aorta in AS mice.The levels of serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were detected by automatic biochemical analyzer.The levels of inflammatory factor interleukin-1β(IL-1β)were detected by enzyme linked immunosorbent assay.The expression of TLR4,NF-κB and NLRP3 proteins in aortic tissue was detected by immunohistochemistry.RESULTS:Compared with the MOD,the levels of serum TC,TG and LDL-C in the JZXB-H and ATO were significantly decreased,while the level of HDL-C was significantly increased.The levels of serum TG,LDL-C in the JZXB-M were significantly decreased,and the level of HDL-C was significantly increased.Compared with the MOD,the levels of IL-1βwere significantly decreased,aortic lesions were significantly improved,and the expression of TLR4,NF-κB,and NLRP3 proteins in the aortic tissue was significantly decreased in the JZXB-H,JZXB-M,and ATO.CONCLUSION:JZXB has inhibitory effect on atherosclerosis in mice,and its mechanism may be through regulating the TLR4/NF-κB/NLRP3 signaling pathway and reducing the inflammatory response,so as to play a role in inhibiting atherosclerosis. | LIU Huihui FENG Jun LIU Jianhe CHENG Choufu HU Guoheng | 2024 | Journal of Traditional Chinese Medicine2024，44，1： | 0 |
| 19 | GA-BP Air Quality Evaluation Method Based on Fuzzy Theory显示文摘With the rapid development of China’s economy,the scale of the city has been continuously expanding,industrial enterprises have been increasing,the discharge of multiple pollutants has reached the top of the world,and the environmental problems become more and more serious.The air pollution problem is particularly prominent.Air quality has become a daily concern for people.In order to control air pollution,it is necessary to grasp the air quality situation in an all-round way.It is necessary to evaluate air quality.Accurate results of air quality evaluation can help people know more about air quality.In this paper,refers to previous research results and different evaluation methods,combined with artificial neural network,fuzzy theory,genetic algorithm,GA-BP hybrid algorithm based on fuzzy theory is proposed to evaluate air quality.At the same time,for the problem that the two-grade standard of air quality annual evaluation is not suitable for practical application,the four-grade standard for annual air quality evaluation has been proposed,and its practicality has been verified through experiments.By setting contrast experiments and comparing the air quality evaluation model based on standard BP algorithm,it is proved that the fuzzy GA-BP evaluation model is better than the standard BP model,both in efficiency and accuracy. | Ma Ning Jianhe Guan Pingzeng Liu Ziqing Zhang Gregory M.P.O’Hare | 2019 | Computers, Materials & Continua2019，，1： | 0 |
| 20 | Temporal characteristics of agarwood formation in Aquilaria sinensis after applying whole-tree agarwood-inducing technique显示文摘Objective: Agarwood—a resinous wood produced by Aquilaria plants in response to injury or artificial induction—is a valuable medicinal and fragrance resource. Whole-Tree Agarwood-Inducing Technique(Agar-WIT) has been widely used to produce agarwood. However, the time-dependent characteristics of agarwood formation induced by Agar-WIT are yet to be clarified. To promote technologically efficient utilization and upgradation of Agar-WIT, the dynamic process and mechanism of agarwood formation were analyzed for one year.Methods: Agarwood formation percentage, barrier layer microscopic properties, extract levels, compound level, and characteristic chromatograms of agarwood were examined by referring to the Chinese Pharmacopeia(2020 version).Results: Agar-WIT could maintain a high percentage of agarwood formation over one year compared with that of healthy plants. Alcohol-soluble extract and agarotetrol levels showed fluctuating cyclic changes with peaks occurring first during the fifth and sixth months, and subsequently in the 11th month.Aquilaria trees subjected to Agar-WIT treatment for 1–12 months showed significant characteristics of a dynamic agarwood formation process. The barrier layer began to appear in the fourth month after treatment. Alcohol-soluble extractive levels in agarwood formed in the second month, and thereafter,exceeded 10.0%, and agarotetrol in agarwood produced after four months or later, exceeded 0.10%.Conclusion: According to the Chinese Pharmacopoeia, alcohol-soluble extractive levels in agarwood should not be less than 10.0% and agarotetrol level should exceed 0.10%. After four months of Agar-WIT treatment, the formed agarwood theoretically met these standards and was suitable for developed and utilization. However, the optimal harvest time was found to be the 11th month, followed by the sixth month after Agar-WIT treatment. Therefore, Agar-WIT resulted in swift agarwood formation and stable accumulation of alcohol-soluble extracts and agarotetrol. Thus, this method is efficient for large-scale cultivation of Aquilaria sinensis to produce agarwood and provide raw materials for the agarwood medicinal industry. | Yan Zhang Hui Meng Feifei Lyu Xiaohong Fan Peiwei Liu Xin He Ying Huang Bo Chen Yun Yang Jianhe Wei | 2023 | Chinese Herbal Medicines2023，15，1： | 0 |
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