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| 1 | Size,composition,and mixing state of individual aerosol particles in a South China coastal city显示文摘Aerosol samples were collected in summer in Macao,a coastal city of the Pearl River Delta Region in China.Morphology,size,elemental composition,and mixing state of individual aerosol particles were determined by scanning electron microscopy coupled energy dispersive X-ray(SEM/EDX) and transmission electron microscopy(TEM).Based on the morphologies of 5711 aerosol particles,they consist of soot(32%),mineral(17%),secondary(22%),and unknown fine particles(29%).The sizes of these particles were mostly distributed between 0.1 and 0.4 μm.Compositions of 202 mineral particles were obtained by SEM/EDX.Mineral particles were mainly classified into three types:Si-rich,Ca-rich,and Na-rich.The compositions of typical mineral particles can indicate their sources in sampling location.For example,mineral particles,collected along the main street,were associated with trace amounts of heavy metals,such as Zn,Ti,Mn,Ba,Pb,and As.TEM observations indicate that most Na-rich particles were aged sea salt particles(e.g.,Na2SO4 and NaNO3) which formed through heterogeneous chemical reactions between sea salt and acidic gases.Additionally,aging time of soot was short in Macao due to high humidity,high temperature,and high levels of sunlight in Macao.Most of soot and fine mineral dust particles were internally mixed with secondary particles. | Weijun Li Longyi Shao Zhishi Wang Rongrong Shen Shusheng Yang Uwa Tang | 2010 | Journal of Environmental Sciences2010，22，4： | 21 |
| 2 | Whole-Genome Sequencing and Analysis of the Chinese Herbal Plant Panax notoginseng显示文摘 | Wei Chen Ling Kui Guanghui Zhang Shusheng Zhu Jing Zhang Xiao Wang Min Yang Huichuan Huang Yixiang Liu Yong Wang Yahe Li Lipin Zeng Wen Wang Xiahong He Yang Dong Shengchao Yang | 2017 | Molecular Plant2017，10，6： | 21 |
| 3 | A Review on Graphene-Based Gas/Vapor Sensors with Unique Properties and Potential Applications显示文摘Graphene-based gas/vapor sensors have attracted much attention in recent years due to their variety of structures, unique sensing performances, room-temperature working conditions, and tremendous application prospects, etc.Herein, we summarize recent advantages in graphene preparation, sensor construction, and sensing properties of various graphene-based gas/vapor sensors, such as NH_3, NO_2, H_2, CO, SO_2, H_2S, as well as vapor of volatile organic compounds.The detection mechanisms pertaining to various gases are also discussed. In conclusion part, some existing problems which may hinder the sensor applications are presented. Several possible methods to solve these problems are proposed, for example, conceived solutions, hybrid nanostructures, multiple sensor arrays, and new recognition algorithm. | Tao Wang Da Huang Zhi Yang Shusheng Xu Guili He Xiaolin Li Nantao Hu Guilin Yin Dannong He Liying Zhang | 2016 | Nano-Micro Letters2016，8，2： | 18 |
| 4 | Binding Activity Difference of Anti-CD20 scFv-Fc Fusion Protein Derived from Variable Domain Exchange显示文摘Two novel engineered antibody fragments binding to antigen CD20 were generated by fusing a murine IgM-type anti-CD20 single-chain Fv fragment (scFv) to the human IgG1 CH2 (i.e., Cγ2) and CH3 (i.e., Cγ3) domains with the human IgG1 hinge (i.e. Hγ). Given the relationship between structure and function of protein, the 3-D structures of the two engineered antibody fragments were modeled using computer-aided homology modeling method. Furthermore, the relationship between 3-D conformation and their binding activity was evaluated theoretically. Due to the change of active pocket formed by CDRs, the HL23 (VH-Linker-VL-Hγ-Cγ2-Cγ3) remained its activity because of its preserved conformation, while the binding activity of the LH23 (VL-Linker-VH-Hγ-Cγ2-Cγ3) was impaired severely. Experimental studies by flow cytometry and fluorescence microscopy showed that HL23 possessed significantly superior binding activity to CD20-expressing target cells than LH23. That is to say, the order of variable regions could influence the binding activity of the fusion protein to CD20+ cell lines, which was in accordance with the theoretical results. The study highlights the potential relationship between the antibody binding activity and their 3-D conformation, which appears to be worthwhile in providing direction for future antibody design of recombinant antibody. | Shusheng Geng Jiannan Feng Yan Li Yingxun Sun Xin Gu Ying Huang Yugang Wang Xianjiang Kang Hong Chang Beifen Shen | 2006 | Cellular & Molecular Immunology2006，3，6： | 13 |
| 5 | A Novel Method of Newton Iteration in Complex Field and Lattice Search for Locating Partial Discharges in Transformers显示文摘在变压器局部放电特高频定位的多传感器时间差测量中不可避免地存在误差,这往往导致时间差方程组在实数域内无解。为了得到时间差方程组的最优近似解,提出了复数域牛顿迭代网格搜索方法。该方法在复数域内进行迭代;当迭代结果为实数时就将其作为定位结果;当迭代结果为复数时,以其实部为中心点坐标,在该点周围局部区域内采用网格搜索法计算最优近似解。通过在变压器上开展试验检验了该方法的可行性和准确性。研究表明,在一定时间误差下,在实数域采用牛顿迭代法计算时不收敛,而在复数域可以收敛,平均定位误差为0.20 m。复数域牛顿迭代网格搜索方法能够在一定时间误差下求解时间差方程组,给出最优近似解,实现局部放电定位。 | ZHENG Shusheng LI Chengrong HE Meng | 2013 | 中国电机工程学报2013，33，9： | 9 |
| 6 | A unified approach to type synthesis of both rigid and flexure parallel mechanisms显示文摘Type synthesis of both rigid and compliant parallel mechanisms has become a hot issue in the field of mechanisms and robotics research in recent years. A unified approach to type synthesis of the two classes of mechanisms, however, has not been referred and investigated up to date. Based on the state-of-art analysis for several major type synthesis approaches related to rigid and compliant mechanisms, respectively, it proves feasible to establish a unified methodology for type synthesis of these two classes of mechanisms. That is a synthesis philosophy in terms of the hierarchy mapping between mathematic, physical, and mechanical building blocks in the framework of screw theory, as addressed in this paper. The key point of the proposed method lies in establishing the mapping among three different building blocks (i.e. geometric building block, kinematic or constraint building block, and mechanical building block). As a result, it makes the whole type synthesis process simple and visible. By using the proposed method, two examples are taken to verify the effectiveness for the type synthesis of both rigid and flexure mechanisms. The content of this paper may provide a theoretical frame for constructing a visualized algorithm or software about the unified type synthesis (or conceptual design) of both rigid and flexure parallel mechanisms. | YU JingJun LI ShouZhong PEI Xu BI ShuSheng ZONG GuangHua | 2011 | Science China(Technological Sciences)2011，54，5： | 8 |
| 7 | Time-varying fault-tolerant formation tracking based cooperative control and guidance for multiple cruise missile systems under actuator failures and directed topologies显示文摘This paper studies time-varying fault-tolerant formation tracking problems for the multiple cruise missile system under directed topologies subjected to actuator failures. Firstly, the timevarying fault-tolerant formation tracking process for the multiple cruise missile system is divided into the guidance loop and the control loop. Then protocols are constructed to accomplish distributed fault-tolerant formation tracking in the guidance loop with the adaptive updating mechanism, in the condition where neither the knowledge about actuator malfunctions nor any global information of the communication topology remains available. Moreover, sufficient conditions to accomplish formation tracking are presented, and it is shown that the multiple cruise missile system can carry on the predefined time-varying fault-tolerant control (FTC) formation tracking through the active disturbances rejection controller (ADRC) and the proportion integration (PI) controller by the way of the fault-tolerant protocol utilizing the designed strategies, in the event of actuator failures. At last, numerical analysis and simulation are designed to verify the theoretical results. | XU Xingguang WEI Zhenyan REN Zhang LI Shusheng | 2019 | Journal of Systems Engineering and Electronics2019，30，3： | 8 |
| 8 | Atomic Fe-Zn dual-metal sites for high-efficiency pH-universal oxygen reduction catalysis显示文摘An effective electrocatalyst being highly active in all pH range for oxygen reduction reaction(ORR)is crucial for energy conversion and storage devices.However,most of the high-efficiency ORR catalysis was reported in alkaline conditions.Herein,we demonstrated the preparation of atomically dispersed Fe-Zn pairs anchored on porous N-doped carbon frameworks(Fe-Zn-SA/NC),which works efficiently as ORR catalyst in the whole pH range.It achieves high half-wave potentials of 0.78,0.85 and 0.72 V in 0.1 M HClO4,0.1 M KOH and 0.1 M phosphate buffer saline(PBS)solutions,respectively,as well as respectable stability.The performances are even comparable to Pt/C.Furthermore,when assembled into a Zn-air battery,the high power density of 167.2 mWcm−2 and 120 h durability reveal the feasibility of Fe-Zn-SA/NC in real energy-related devices.Theoretical calculations demonstrate that the superior catalytic activity of Fe-Zn-SA/NC can be contributed to the lower energy barriers of ORR at the Fe-Zn-N6 centers.This work demonstrates the potential of Fe-Zn pairs as alternatives to the Pt catalysts for efficient catalytic ORR and provides new insights of dual-atom catalysts for other energy conversion related catalytic reactions. | Jie Xu Shuhua Lai Defeng Qi Min Hu Xianyun Peng Yifan Liu Wei Liu Guangzhi Hu Heng Xu Fan Li Chao Li Jia He Longchao Zhuo Jiaqiang Sun Yuan Qiu Shusheng Zhang Jun Luo Xijun Liu | 2021 | Nano Research2021，14，5： | 6 |
| 9 | Recombinant vaccine containing an RBD-Fc fusion induced protection against SARS-CoV-2 in nonhuman primates and mice显示文摘The novel coronavirus SARS-CoV-2 has infected more than 104 million individuals and resulted in more than 2.2 million deaths worldwide as of February 7,2021(https://covid19.who.int).The COVID-19 pandemic highlights the need for safe and effective vaccines against SARS-CoV-2 infection. | Shihui Sun Lei He Zhongpeng Zhao Hongjing Gu Xin Fang Tiecheng Wang Xiaolan Yang Shaolong Chen Yongqiang Deng Jiangfan Li Jian Zhao Liang Li Xinwang Li Peng He Ge Li Hao Li Yuee Zhao Chunrun Gao Xiaoling Lang Xin Wang Guoqiang Fei Yan Li Shusheng Geng Yuwei Gao Wenjin Wei Zhongyu Hu Gencheng Han Yansong Sun | 2021 | Cellular & Molecular Immunology2021，18，4： | 5 |
| 10 | TIPE1 suppresses the invasion and migration of breast cancer cells and inhibits epithelial-to-mesenchymal transition primarily via the ERK signaling pathway显示文摘Tumor necrosis factor a-induced protein 8-like-1(TIPE1)functions as an activator or a repressor in a tumorcell type-specific manner.However,the role of TIPE1 in breast cancer,especially regarding metastasis,is unknown.In this study,we aimed to investigate the TIPE1 expression in breast cancer tissues,the biological functions,and the underlying mechanisms of TIPE1 regarding the metastatic properties of breast cancer cells.The results of immunohistochem ical staining and western blot analysis indicated that TIPE1 expression was associated with tumor size and lymph node metastasis,and the expression of TIPE1 was downregulated in the tissues of patients w ith lymph node metastasis.Transwell and w ound healing assay results showed that TIPE1 inhibited the invasive and m igratory capacities of breast cancer cells.Moreover,the epithelial-mesenchym al transition(EMT)was suppressed in TIPE1-overexpressing cells,as demonstrated by western blot analysis.In addition,western blot analysis also showed that TIPE1 reduced the expression levels of MMP2 and MMP9 and decreased the phosphorylation level of ERK.These results suggested that TIPE1 m ight suppress the invasion and m igration of breast cancer cells and inhibit EMT prim arily via the ERK signaling pathway.Our findings revealed the anti-tum or metastasis role of TIPE1 in breast cancer and TIPE1 might be a new candidate prognostic indicator and a potential m olecular target fo r the treatm ent of breast cancer. | Shusheng Qiu Wei Hu Qiuhong Ma Yi Zhao Liang Li Yu Ding | 2019 | Acta Biochimica et Biophysica Sinica2019，51，10： | 4 |
| 11 | Induction of xenogeneic islet transplantation tolerance by simultaneously blocking CD28-B7 and OX40-OX40L co-stimulatory pathways显示文摘It has been demonstrated that prolonged graft survival can be achieved through inhibiting the activation of T cells, and addition of soluble CTLA4Ig and OX40Ig proteins to mixed lymphocyte reactions can effectively inhibit T cell proliferation. To explore the potential of this type of treatment in xenotransplantation, we infected streptozotocin-induced diabetic BalB/c mice (H-2d) (200 mg/kg, IV) with 5×108 pfu AdCTLA4Ig-IRES-OX40Ig on day 1 before islets trans-plantation through the tail vein. The results showed that this treatment prolonged the islet xeno-grafts survival significantly. The reaction to exogenous glucose stimulation was normal and the cytokine secretion of the type Th1 cells was inhibited. The AdCTLA4Ig-IRES-OX40Ig-mediated treatment effectively induced the T cells into anergy and the Th1/Th2 cells into deviation. These results strongly supported the therapeutic potential of blockade of costimulation by Ad-CTLA4Ig-IRES-OX40Ig genes transfer in inducing the organ transplantation tolerance. | WANG Guangming FENG Yougang HAO Jie LI Ailing GAO Xiang XIE Shusheng | 2005 | Science China(Life Sciences)2005，48，5： | 4 |
| 12 | INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE显示文摘Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases.The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations.Interactions between fennel(Foeniculum vulgare)roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco(Nicotiana tabacum).The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P.nicotianae were assessed.The roots of fennel attracted P.nicotianae zoospores and inhibited their motility and the germination of cystospores,with some cystospores rupturing.4-ethylacetophenone,vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P.nicotianae,especially vanillin.Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species.These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P.nicotianae. | Yuxin YANG He ZHANG Yuting FANG Ying LI Xinyue MEI Huichuan HUANG Fei DU Shusheng ZHU Min YANG Yixiang LIU | 2021 | Frontiers of Agricultural Science and Engineering2021，8，3： | 3 |
| 13 | Tenofovir vs.entecavir on recurrence of hepatitis B virus-related hepatocellular carcinoma beyond Milan criteria after hepatectomy显示文摘Background:Hepatectomy for hepatocellular carcinoma(HCC)beyond the Milan criteria is shown to be beneficial.However,a high rate of post-operative HCC recurrence hinders the long-term survival of the patients.This study aimed to investigate and compare the impacts of tenofovir(TDF)and entecavir(ETV)on the recurrence of hepatitis B viral(HBV)-related HCC beyond the Milan criteria.Methods:Data pertaining to 1532 patients who underwent hepatectomy and received antiviral therapy between January 2014 and January 2019 were collected from five centers.Recurrence-free survival(RFS)analysis was performed using the Kaplan-Meier method.Cox proportional hazards regression analysis was performed to determine prognostic factors for HCC recurrence.Results:The analysis incorporates 595 HBV-related HCC patients.The overall 5-year RFS was 21.3%.Among them,533 and 62 patients received ETV and TDF treatment,respectively.The 1-,3-,and 5-year RFS rates were 46.3%,27.4%,and 19.6%,respectively,in the ETV group compared with 65.1%,41.8%,and 37.2%,respectively,in the TDF group(P<0.001).Multivariate analysis showed that TDF treatment(hazard ratio[HR]:0.604,P=0.005),cirrhosis(HR:1.557,P=0.004),tumor size(HR:1.037,P=0.008),microvascular invasion(MVI)(HR:1.403,P=0.002),portal vein tumor thrombus(PVTT)(HR:1.358,P=0.012),capsular invasion(HR:1.228,P=0.040),and creatinine levels(CREA)(HR:0.993,P=0.031)were statistically significant prognostic factors associated with RFS.Conclusions:Patients with HCC beyond the Milan criteria exhibited a high rate of HCC recurrence after hepatectomy.Compared to the ETV therapy,TDF administration significantly lowered the risk of HCC recurrence. | Junyi Shen Weili Qi Junlong Dai Shusheng Leng Kangyi Jiang Yu Zhang Shun Ran Chuan Li Tianfu Wen | 2022 | Chinese Medical Journal2022，，3： | 3 |
| 14 | Effect of anterior tympanomeatal angle blunting on the middle ear transfer function using a finite element ear model显示文摘 | Jie Wang Fei Zhao Yongxin Li Demin Han Shusheng Gong Shouqin Zhao Hua Zhang | 2011 | Medical Engineering and Physics2011，，9： | 3 |
| 15 | Geology, Geochronology, and Hf Isotopic Composition of the Pha Lek Fe Deposit, Northern Laos: Implications for Early Permian Subduction-Related Skarn Fe Mineralization in the Truong Son Belt显示文摘The Truong Son metallogenic belt in central Laos and Vietnam is an important Fe-Cu-Sn-Au polymetallic ore district. The Pha Lek Fe deposit is closely related to Late Carboniferous– Early Permian I-type granitic magmatism, and contains >50 Mt @ 45% to 50% of Fe ore. Ore minerals occur mainly as magnetite and hematite in the skarn alteration zone between a granitic pluton and metamorphosed Middle–Upper Devonian carbonates. The granitic pluton comprises granodiorite and granite, with zircon U-Pb dating indicating synchronous emplacement at 288.2±1.3 and 284.9±1.2 Ma, respectively. Zircons from these granitoids have ε_(Hf)(t) values of 2.9–11.2 and relatively young TDM2 ages(<1.0 Ga), indicating an origin by partial melting of depleted mafic crust or magma mixing. Previous studies have shown that these granitoids have high Y, Yb, and K_2O contents, and low Sr and Na_2O contents, which are interpreted as the melting of mafic continental crust. Pyrite of the main mineralization stage yields an ^(187)Re/^(188)Os-^(187)Os/^(188)Os isochron age of 287±17 Ma, indicating that mineralization is associated with Pha Lek granitic magmatism. A Late Carboniferous–Early Permian subduction-related skarn-type Fe mineralization model is proposed for the Pha Lek deposit. More evidence is needed to verify a hypothesis of volcanic overprinting during Late Triassic post-collisional extension. | Lin Hou Shusheng Liu Linnan Guo Fuhao Xiong Chao Li Meifeng Shi Qiming Zhang Siwei Xu Songyang Wu | 2019 | Journal of Earth Science2019，30，1： | 2 |
| 16 | The number of births in China in 2015: policy meets superstition显示文摘Fertility in China was long constrained by the One-Child policy enacted in 1978. Over time the policy was progressively relaxed,first in rural areas and then for couples who both had no siblings.A Selective Two-Child policy which started in January-June 2014allowed a second child for couples for whom one spouse was an only child. Now with the Universal Two-Child policy, as of | Hong-Tian Li Susan Hellerstein Chuyun Kang Leonardo Trasande Yanqiu Gao Yali Zhang Shusheng Luo Yu-Bo Zhou Jie Qiao Jan Blustein Jian-Meng Liu | 2018 | Science Bulletin2018，63，19： | 2 |
| 17 | Reduced Thiol Compounds-Induced Biosensing,Bioimaging Analysis and Targeted Delivery显示文摘Reduced thiol compounds(RTCs),such as glutathione(GSH),cysteine(Cys),and homocysteine(Hcy),are important components of many amino acids andproteins.They are also basic buiding blocks that make up organisms and play an important role in physiology and pathology.Therefore,based on thefundamental characteristics of RTCs in vivo,researchers have reported their use in biosensing and imaging analysis and as carriers inlfe sciences andhealth sciences.n this review,we will itroduce the principles of RTLs and highlight their applications in biosensing and imaging analysis and as carriersreported from 2015 to 2020. | Yingshu Guo Xiaofei Zheng Xiuping Cao Wenxin Li Di Wu Shusheng Zhang | 2020 | Chinese Journal of Chemistry2020，38，12： | 2 |
| 18 | Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid显示文摘Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms under-lying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin bio-synthesis in P. notoginseng. Acremonium sp. D212 could secrete indole-3-acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. noto-ginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2–15μmol/L) and 1-naphthalenacetic acid (NAA) (10–20μmol/L). Moreover, the roots of the JA signaling-defective coi1-18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild-type Nipponbare and miR393b-overexpressing lines, and the colonization was res-cued by MeJA but not by NAA. It suggests that the cross-talk between JA signaling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants. | Li Han Xuan Zhou Yiting Zhao Shusheng Zhu Lixia Wu Yunlu He Xiangrui Ping Xinqi Lu Wuying Huang Jie Qian Lina Zhang Xi Jiang Dan Zhu Chongyu Luo Saijie Li Qian Dong Qijing Fu Kaiyuan Deng Xin Wang Lei Wang Sheng Peng Jinsong Wu Weimin Li JiǐíFriml Youyong Zhu Xiahong He Yunlong Du | 2020 | Journal of Integrative Plant Biology2020，62，9： | 2 |
| 19 | Bayesian uncertainty analysis of SA turbulence model for supersonic jet interaction simulations显示文摘The Reynolds Averaged Navier-Stokes(RANS) models are still the workhorse in current engineering applications due to its high efficiency and robustness. However, the closure coefficients of RANS turbulence models are determined by model builders according to some simple fundamental flows, and the suggested values may not be applicable to complex flows, especially supersonic jet interaction flow. In this work, the Bayesian method is employed to recalibrate the closure coefficients of Spalart-Allmaras(SA) turbulence model to improve its performance in supersonic jet interaction problem and quantify the uncertainty of wall pressure and separation length. The embedded model error approach is applied to the Bayesian uncertainty analysis. Firstly, the total Sobol index is calculated by non-intrusive polynomial chaos method to represent the sensitivity of wall pressure and separation length to model parameters. Then, the pressure data and the separation length are respectively served as calibration data to get the posterior uncertainty of model parameters and Quantities of Interests(Qo Is). The results show that the relative error of the wall pressure predicted by the SA turbulence model can be reduced from 14.99% to 2.95% through effective Bayesian parameter estimation. Besides, the calibration effects of four likelihood functions are systematically evaluated. The posterior uncertainties of wall pressure and separation length estimated by different likelihood functions are significantly discrepant, and the Maximum a Posteriori(MAP) values of parameters inferred by all functions show better performance than the nominal values. Finally, the closure coefficients are also estimated at different jet total pressures. The similar posterior distributions of model parameters are obtained in different cases, and the MAP values of parameters calibrated in one case are also applicable to other cases. | Jinping LI Shusheng CHEN Fangjie CAI Sheng WANG Chao YAN | 2022 | Chinese Journal of Aeronautics2022，35，4： | 2 |
| 20 | Differential efficiency of a begomovirus to cross the midgut of different species of whiteflies results in variation of virus transmission by the vectors显示文摘Begomoviruses are important crop viral disease agents, and they are transmitted by whiteflies of the Bemisia tabaci complex.Although the transmission of begomoviruses by whiteflies has been studied for many years, the mechanisms governing differential transmission of begomoviruses by different species of the Bemisia tabaci complex remain largely unknown. Here we firstly compared the transmission efficiency of tobacco curly shoot virus(TbCSV) by four species of the B. tabaci complex and found that Asia II 1 transmitted this virus with the highest efficiency, whereas MEAM1 transmitted it with the lowest. Next, by performing quantitative analysis of virus and immune-fluorescence detection, we found that the efficiency of TbCSV to cross the midgut wall was higher in Asia II 1 than in MEAM1. Finally, we set the quantities of virions in the haemolymph to the same level in Asia II 1 and MEAM1 via injection and then compared their capacity in TbCSV transmission, and found that the difference in TbCSV transmission between them became smaller. Taken together, our findings suggest that the efficiency of a begomovirus to cross the midgut wall of a whitefly to reach the vector's haemolymph plays a significant role in determining transmission of the virus. | Lilong Pan Qunfang Chen Tao Guo Xinru Wang Ping Li Xiaowei Wang Shusheng Liu | 2018 | Science China(Life Sciences)2018，61，10： | 2 |
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