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| 1 | Ascorbic acid enhances the cardiac differentiation of induced pluripotent stem cells through promoting the proliferation of cardiac progenitor cells显示文摘导致的 pluripotent 干细胞(iPSCs ) 的产生为心疾病,屏蔽的药和潜在的自体同源的心脏的新生的调查打开了新大街。然而,他们的申请被导出 iPSC 的 cardiomyocytes (iPS 厘米) 的低效的心脏的区别,高写在行间可变性,和差的成熟妨碍。为 iPSCs 的心脏的区别和成熟识别有效 inducers 并且阐明机制,我们系统地在各种各样的鼠科的(m) iPSCs 上屏蔽了十六 cardiomyocyte inducers 并且发现没有自发的心脏性的潜力,仅仅维生素酸(AA ) 一致地并且要用体力地包括八提高了十一根线的心脏的区别。我们然后优化了治疗条件并且表明了那区别天 2-6,为心脏的祖先房间(CPC ) 的说明的经期,是一批评时间让 AA 生效。这被 AA 增加了表示的事实进一步证实心血管然而并非 mesodermal 标记。显著, AA 处理导致了近似 7.3 褶层(miPSCs ) 和 30.2 褶层(人的 iPSCs ) 在 iPS 厘米的收益扩充。如此的效果经由 MEK-ERK1/2 小径被归因于 CPC 的增长的特定的增加由通过支持骨胶原合成。另外,导致 AA 的 cardiomyocytes 显示出更好的 sarcomeric 组织并且提高了行动潜力和钙 transients 的回答到 β肾上腺素能并且 muscarinic 刺激。这些调查结果证明 AA 是合适的 cardiomyocyte inducer 让 iPSCs 普遍,简单地改进心脏的区别和成熟。这些调查结果也强调由在指导 pluripotent 干细胞的心脏的区别操作细胞外的微型环境刺激 CPC 增长的重要性。 | Nan Cao Zumei Liu Zhongyan Chen Jia Wang Taotao Chen Xiaoyang Zhao Yu Ma Lianju Qin Jiuhong Kang Bin Wei Liu Wang Ying Jin Huang-Tian Yang | 2012 | Cell Research2012，22，1： | 28 |
| 2 | Empirical Analysis on Influence Mechanism of Cohesiveness and Collective Efficacy of Virtual Community Based on Triadic Reciprocal Determinism显示文摘The virtual community has become a new life style as for human,but the public's acceptance is relatively low at present.So the research about the mechanism of the influence on the cohesiveness and the collective efficiency in virtual community has an important theoretical value and practical value.In the paper,the key variables that affect virtual community cohesiveness are extracted from two aspects—self-perception of community members and community environment,and the influence mechanism model of virtual community cohesiveness and community collective efficiency is established based on Triadic Reciprocal Determinism.By means of the questionnaire on the community members and the empirical analysis,the key factors influencing cohesiveness of virtual community are brought up,as well as the mechanism that the four dimensions of cohesiveness work,on collective efficiency.According to the results,it is practically meaningful that the problems and insufficiency in the operation of existing virtual community are discovered,which guides the operating managers of the virtual community to formulate operation policy of the virtual community for operating managers to develop strategies. | NING Lianju LIU Qian XIA Wen | 2014 | China Communications2014，11，11： | 2 |
| 3 | Retinoic acid can improve autophagy through depression of the PI3K-Akt-mTOR signaling pathway via RARαto restore spermatogenesis in cryptorchid infertile rats显示文摘Cryptorchidism-caused adult infertility is a common component of idiopathic reasons for male infertility.Retinoic acid(RA)has a vital effect on the spermatogenesis process.Here,we found that the expression of c-Kit,Stra8,and Sycp3 could be up-regulated via the activation of retinoic acid receptorα(RARα)after RA supplementation in neonatal cryptorchid infertile rats.We also demonstrated that the protein expression of PI3K,p-Akt/pan-Akt,and p-mTOR/mTOR was higher in cryptorchid than in normal testes,and could be suppressed with RA in vivo.After RA treatment in infertile cryptorchid testis in vivo,the levels of the autophagy proteins LC3 and Beclin1 increased and those of P62 decreased.Biotin tracer indicated that the permeability of blood-testis barrier(BTB)in cryptorchid rats decreased after RA administration.Additionally,after blocking the RARαwith AR7(an RARαantagonist)in testicle culture in vitro,we observed that compared with normal testes,the PI3K-Akt-mTOR signaling pathway and the autophagy pathway was increased and decreased,respectively,which were coincident with cryptorchisd testes in vivo.Additionally,the appropriate concentrations of RA treatment could depress the PI3K-Akt-mTOR signaling pathway and improve the autophagy pathway.The results confirmed that RA can rehabilitate BTB function and drive key protein levels in spermatogonial differentiation through depressing the PI3K-Akt-mTOR signaling pathway via RARα. | Chunlan Long Yu Zhou Lianju Shen Yihang Yu Dong Hu Xing Liu Tao Lin Dawei He Tao Xu Deying Zhang Jing Zhu Guanghui Wei | 2022 | Genes & Diseases2022，9，5： | 2 |
| 4 | The Browsing Pattern and Review Model of Online Consumers Based on Large Data Analysis显示文摘In the context of online shopping, commodity information and consumer reviews are main factors that will affect purchasing behavior. Started from the preference of commodity information browsing and the inherent property of online reviews, this paper focuses on the browsing data for statistical analysis and the interval distribution of consumer reviews based on the real data of360 buy which is the domestic large-scale B2 C commerce website in China. Researches find that commodity information browsing time distribution on Internet is fragment and can be depicted by the fat tail effect. It also demonstrated that user's browsing patterns are related to the type of information and the displaying of the information,which means that the pieces of the picture and the length of the titles affecting the clicks rate. Reviews on the interval distribution can be depicted by the power-law function and there is a monotonically increasing relationship between power-exponent and the customers' concerns with the corresponding commodity, the higher the exponent,the higher the degree of consumer attention. The finding obtained some basic rules of the browsing mode and review model, which is of important significance for future research. | NING Lianju WANG Haoyu FENG Xin DU Junping | 2015 | Chinese Journal of Electronics2015，24，1： | 2 |
| 5 | Empirical Analysis on Factors Af- fecting Mobile Internet Users' Purchase Intention for 3G Handsets 显示文摘 | NING lianju XIA Wen | 2011 | China Communications2011，8，3： | 1 |
| 6 | Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties显示文摘 | Haifeng Zhao Wei Fan Jianjun Dong Jian Lu Jian Chen Lianju Shan Yan Lin Weibao Kong | 2007 | Food Chemistry2007，，1： | 1 |
| 7 | Pretreatment with Na Cl induces tolerance of rice seedlings to subsequent Cd or Cd+Na Cl stress显示文摘 | Ma Lianju Yu Cuimei Li Xuemei | 2013 | Biologia Plantarum2013，57，3： | 1 |
| 8 | Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress显示文摘 | Ma Lianju Li Yueying Yu Cuimei | 2012 | Protoplasma2012，249，： | 1 |
| 9 | Primary Study on Experiment of Tetraploid Induction in Haliotis discus hannai with PEG显示文摘The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 (34)] was used. Three factors and three levels were molecular weight of PEG: 8 000, 6 000, 4 000 mol·g-1; PEG concentration: 45%, 50%, 55%; treatment duration time: 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were: molecular weight 4 000, concentration 55%, treatment duration time: 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time→concentration→molecular weight. | LI Yajuan MAO Lianju LI Xia WANG Zichen YU Yi | 2006 | Journal of Northeast Agricultural University(English Edition)2006，13，1： | 1 |
| 10 | Pretreatment with NaC1 induces tolerance of rice seedlings to subsequent Cd or Cd+NaC1 stress显示文摘 | MA LIANJU YU CUIMEI LI XUEMEI | 2013 | Biol Plant2013，57，3： | 1 |
| 11 | Bioinformatic identification of key genes and molecular pathways in the spermatogenic process of cryptorchidism显示文摘This study aims to determine key genes and pathways that could play important roles in the spermatogenic process of patients with cryptorchidism.The gene expression profile data of GSE25518 was obtained from the Gene Expression Omnibus(GEO)database.Microarray data were analyzed using BRB-Array Tools to identify differentially expressed genes(DEGs)between high azoospermia risk(HAZR)patients and controls.In addition,other analytical methods were deployed,including hierarchical clustering analysis,class comparison between patients with HAZR and the normal control group,gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and the construction of a proteineprotein interaction(PPI)network.In total,1015 upregulated genes and 1650 downregulated genes were identified.GO and KEGG analysis revealed enrichment in terms of changes in the endoplasmic reticulum cellular component and the endoplasmic reticulum protein synthetic process in the HAZR group.Furthermore,the arachidonic acid pathway and mTOR pathway were also identified as important pathways,while RICTOR and GPX8 were indentified as key genes involved in the spermatogenic process of patients with cryptorchidism.In present study,we found that changes in the synthesis of endoplasmic reticulum proteins,arachidonic acid and the mTOR pathway are important in the incidence and spermatogenic process of cryptorchidism.GPX8 and RICTOR were also identified as key genes associated with cryptorchidism.Collectively,these data may provide novel clues with which to explore the precise etiology and mechanism underlying cryptorchidism and cryptorchidism-induced human infertility. | Yu Zhou Deying Zhang Bo Liu Dong Hu Lianju Shen Chunlan Long Yihang Yu Tao Lin Xing Liu Dawei He Guanghui Wei | 2019 | Genes & Diseases2019，6，4： | 1 |
| 12 | Synthesis and structure–activity relationship analysis of bicyclophosphorothionate blockers with selectivity for housefly γ‐aminobutyric acid receptor channels显示文摘 | Xiu‐LianJu SayakaFusazaki HiroyukiHishinuma XiaomuQiao IzumiIkeda YoshihisaOzoe | 2010 | Pest Manag Sci2010，，9： | 1 |
| 13 | A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism显示文摘Calciphylaxis is a rare disease characterized histologically by microvessel calcification and microthrombosis,with high mortality and no proven therapy.Here,we reported a severe uremic calciphylaxis patient with progressive skin ischemia,large areas of painful malodorous ulcers,and mummified legs.Because of the worsening symptoms and signs refractory to conventional therapies,treatment with human amnion-derived mesenchymal stem cells(hAMSCs)was approved.Preclinical release inspections of hAMSCs,efficacy,and safety assessment,including cytokine secretory ability,immunocompetence,tumorigenicity,and genetics analysis in vitro,were introduced.We further performed acute and long-term hAMSC toxicity evaluations in C57BL/6 mice and rats,abnormal immune response tests in C57BL/6 mice,and tumorigenicity tests in neonatal Balbc-nu nude mice.After the preclinical research,the patient was treated with hAMSCs by intravenous and local intramuscular injection and external supernatant application to the ulcers.When followed up to 15 months,the blood-based markers of bone and mineral metabolism improved,with skin soft tissue regeneration and a more favorable profile of peripheral blood mononuclear cells.Skin biopsy after 1-month treatment showed vascular regeneration with mature noncalcified vessels within the dermis,and 20 months later,the re-epithelialization restored the integrity of the damaged site.No infusion or local treatment-related adverse events occurred.Thus,this novel long-term intravenous combined with local treatment with hAMSCs warrants further investigation as a potential regenerative treatment for uremic calciphylaxis due to effects of inhibiting vascular calcification,stimulating angiogenesis and myogenesis,anti-inflammatory and immune modulation,multidifferentiation,re-epithelialization,and restoration of integrity. | Lianju Qin Jing Zhang Yujie Xiao Kang Liu Yugui Cui Fangyan Xu Wenkai Ren Yanggang Yuan Chunyan Jiang Song Ning Xiaoxue Ye Ming Zeng Hanyang Qian Anning Bian Fan Li Guang Yang Shaowen Tang Zhihong Zhang Juncheng Dai Jing Guo Qiang Wang Bin Sun Yifei Ge Chun Ouyang Xueqiang Xu Jing Wang Yaoyu Huang Hongqing Cui Jing Zhou Meilian Wang Zhonglan Su Yan Lu Di Wu Jingping Shi Wei Liu Li Dong Yinbing Pan Baiqiao Zhao Ying Cui Xueyan Gao Zhanhui Gao Xiang Ma Aiqin Chen Jie Wang Meng Cao Qian Cui Li Chen Feng Chen Youjia Yu Qiang Ji Zhiwei Zhang Mufeng Gu Xiaojun Zhuang Xiaolin Lv Hui Wang Yanyan Pan Ling Wang Xianrong Xu Jing Zhao Xiuqin Wang Cuiping Liu Ningxia Liang Changying Xing Jiayin Liu Ningning Wang | 2022 | Journal of Molecular Cell Biology2022，14，2： | 1 |
| 14 | Integrate Fuzzy Logic Stable Control System of Bank-to-Turn Missiles显示文摘The question of stable control system of bank-to-turn (BTT) missiles is a bottleneckin BTT technology. Integrate fuzzy logic stable control system of BTT missiles is designed in whichthree main problems are resolved. How to select input variables Of the fuzzy logic controller and howto guarantee completeness of the output control are two of them. The last one is how to coordinatethe fuzzy logic controllers in integrate fuzzy logic stable control system. Simulating results prov that integrate fuzzy logic stable coatrol system of BTT missiles is sueccessful, and it can be widelyused in future. | Guo Yuchun Sun Lianju Dai Zongli & Jiang Yong(Beijing Institute of Electronic System Engineering, 100854, P. R. China) | 1998 | Journal of Systems Engineering and Electronics1998，9，4： | 0 |
| 15 | Age Structure of Two Species of Odorous Frogs(Odorrana margaretae and Odorrana grahami)显示文摘Variation in age structure and body size benefits are identified to understand the evolution of life history.Here,we estimated the age structure and body size of two species of odorous frogs(Odorrana margaretae and Odorrana grahami)by using skeletochronology.The ages at sexual maturity of O.grahami and O.margaretae in both sexes were 1 and 2 years,respectively.For both sexes,the maximum age observed in O.margaretae was six years.For O.grahami,the maximum age observed in males and females were 4 and 5 years,respectively.Males and females did not differ in mean age in the two species.The average body size of both species considerably differed between sexes,with females being larger than males.The body size of females was also larger than that of males when the effect of age was removed.We also found positive correlations between body size and age within each sex in O.margaretae,but only for female in O.grahami.The female-biased sexual size dimorphism of the two species suggested that fecundity selection for larger female size may increase the reproductive output. | LIANJU YUAN MAOJUN ZHONG WENBO LIAO | 2021 | Asian Herpetological Research2021，12，3： | 0 |
| 16 | Study of matching model between tariff package and user behavior显示文摘As the telecommunication market in China becomes increasingly mature, operators have begun to focus their primary effort on user management; within this focus, determining the proper tariff package for users and offering them relevant recommendations are key issues to resolve. This paper introduces a matching model that links tariff packages and users' usage behavior(e.g., the total minutes used, data usage, etc.) based on the market segmenting theory. Microsoft Visual Fox Pro 9.0 is selected as the development tool to implement the matching model, while the tariff packages and user behavior data for a city branch of China Mobile are used to validate the model. | Xiao Shuochen Ning Lianju Zhuang Wenying | 2017 | The Journal of China Universities of Posts and Telecommunications2017，24，3： | 0 |
| 17 | Yundi Behavior and Health Research Center Joins Hands with Five Neighboring Countries to Carry out Online Training on COVID-19 Community Prevention and Control显示文摘The Belt and Road Initiative has brought rich and diverse possibilities to global cooperation,recommending that countries strengthen multilateral cooperation,help each other,and jointly face and resolve various risks. | Luo Zhi Hong Duo He Lianju | 2020 | International Understanding2020，，3： | 0 |
| 18 | Cell-free synthetic biology in the new era of enzyme engineering显示文摘With the gradual rise of enzyme engineering,it has played an essential role in synthetic biology,medicine,and biomanufacturing.However,due to the limitation of the cell membrane,the complexity of cellular metabolism,the difficulty of controlling the reaction environment,and the toxicity of some metabolic products in traditional in vivo enzyme engineering,it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds.Recently,cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies.This cell-free method has no limitation of the cell membrane and no need to maintain cell viability,and each biosynthetic pathway is highly flexible.This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize.This article aims to discuss the latest advances in cell-free enzyme engineering,assess the trend of this developing topical filed,and analyze its prospects. | Nan Jiang Lianju Ma Yuan Lu | 2020 | Chinese Journal of Chemical Engineering2020，28，11： | 0 |
| 19 | Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system显示文摘Amber suppression has been widely used to incorporate unnatural amino acids(UNAAs)with unique structures or functional side-chain groups into specific sites of the target protein,which expands the scope of protein-coding chemistry.However,this traditional strategy does not allow multiple-site incorporation of different UNAAs into a single protein,which limits the development of unnatural proteins.To address this challenge,the suppression method using multiple termination codons(TAG,TAA or TGA)was proposed,and cell-free unnatural protein synthesis(CFUPS)system was employed.By the analysis of incorporating 3 different UNAAs(p-propargyloxy-L-phenylalanine,p-azyl-phenylalanine and L-4-Iodophenylalanine)and mass spectrometry,the simultaneous usage of the codons TAG and TAA were suggested for better multiple-site UNAA incorporation.The CFUPS conditions were further optimized for better UNAA incorporation efficiency,including the orthogonal translation system(OTS)components,magnesium ions,and the redox environment.This study established a CFUPS approach based on multiple termination codon suppression to achieve efficient and precise incorporation of different types of UNAAs,thereby synthesizing unnatural proteins with novel physicochemical functions. | Jiaqi Hou Xinjie Chen Nan Jiang Yanan Wang Yi Cui Lianju Ma Ying Lin Yuan Lu | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 0 |
| 20 | Antitumor effect of Escherichia coli-derived outer membrane vesicles on neuroblastoma in vitro and in vivo显示文摘Bacterial outer membrane vesicles(OMVs)are spherical microbubbles that contain biological content and are produced by gram-negative bacteria.The use of OMVs as adjuvants for cancer immunotherapy or as drug carriers for targeted therapies has attracted the interest of many scholars.However,it is unclear whether OMVs can exert direct antitumor effects and whether OMVs can inhibit pediatric tumors.Here,we explore the potential of Escherichia coli-derived OMVs to directly suppress neuroblastoma.Our results demonstrate the antitumor effects of OMVs in vitro and in vivo,and no serious adverse reactions were observed.OMV uptake into the cytoplasm and nucleus directly decreases cell stemness,DNA damage,apoptosis and cell cycle arrest,which may be the mechanisms bywhichOMVs suppress tumors.Ourresultsdemonstrate thepotential of bacterial OMVs to be usedas antitumor adjuvant therapies,increasing the number of candidates for the development of cancer therapies in the future.More relevant studies are urgently needed to demonstrate the efficacy and safety of OMVs. | Liming Jin Zhaoxia Zhang Xiaojun Tan Zhaoying Wang Bo Tang Zhang Wang Mujie Li Tao Mi Lianju Shen Chunlan Long Guanghui Wei Dawei He | 2022 | Acta Biochimica et Biophysica Sinica2022，54，9： | 0 |
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