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| 1 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 2 | Substance P-modified human serum albumin nanoparticles loaded with paclitaxel for targeted therapy of glioma显示文摘The blood–brain barrier(BBB) and the poor ability of many drugs to cross that barrier greatly limits the efficacy of chemotherapies for glioblastoma multiforme(GBM). The present study exploits albumin as drug delivery vehicle to promote the chemotherapeutic efficacy of paclitaxel(PTX) by improving the stability and targeting efficiency of PTX/albumin nanoparticles(NPs). Here we characterize PTX-loaded human serum albumin(HSA) NPs stabilized with intramolecular disulfide bonds and modified with substance P(SP) peptide as the targeting ligand. The fabricated SP-HSA-PTX NPs exhibited satisfactory drug-loading content(7.89%) and entrapment efficiency(85.7%) with a spherical structure(about 150 nm) and zeta potential of -12.0 mV. The in vitro drug release from SP-HSA-PTX NPs occurred in a redox-responsive manner. Due to the targeting effect of the SP peptide, cellular uptake of SP-HSA-PTX NPs into brain capillary endothelial cells(BCECs) and U87 cells was greatly improved.The low IC_(50), prolonged survival period and the obvious pro-apoptotic effect shown by TUNEL analysis all demonstrated that the fabricated SP-HSA-PTX NPs showed a satisfactory anti-tumor effect and could serve as a novel strategy for GBM treatment. | Chunhui Ruan Lisha Liu Yifei Lu Yu Zhang Xi He Xinli Chen Yujie Zhang Qinjun Chen Qin Guo Tao Sun Chen Jiang | 2018 | Acta Pharmaceutica Sinica B2018，8，1： | 19 |
| 3 | Jianpijiedu Fang improves survival of hepatocarcinoma mice by affecting phosphatase and tensin homolog, phosphoinositide 3-kinase, and focal adhesion kinase显示文摘OBJECTIVE: To investigate the effect of Jianpijiedu Fang (JPJDF) on phosphatase and tensin homolog (PTEN), phosphoinositide 3-kinase (PI3K), and focal adhesion kinase (FAK), and on the survival of hepatocellular carcinoma (HCC) nude mice. METHODS: Forty male nude mice were randomly divided into 4 groups. Human HCC tissue was implanted in the livers of three groups. After 24 h, the three groups were treated respectively with JPJDF (37.5 g/kg), saline (20 mL/kg) and Tegafur (FT-207, 160 mg/kg) once a day for 10 weeks. The control group without implanting the tissue was concurrently treated with saline (20 mL/kg). The survival data and body weight of all mice were recorded, and expression levels of PTEN, PI3K and FAK in normal tissue and cancer tissue of the livers were eval-uated with immunohistochemical method. RESULTS: The cumulative survival rate of the mice in the JPJDF group was higher than those of the other groups. The rate of weight loss was the lowest in JPJDF group. The survivability and weight loss rate in FT-207 group were the poorest in all groups. The expression intensity of PTEN was higher in normal tissues than in cancer tissues, and lower in the normal tissues of HCC models than in that of mice without HCC. The PTEN expression intensity in normal tissue and cancer tissue from mice treated with FT-207 were lower than that from the mice treated with JPJDF or saline.The expression intensity of PI3K was higher in cancer tissue than in normal tissue. The PI3K expression intensity was the lowest in normal tissue and cancer tissue from mice treated with JPJDF, and the intensity from mice treated with FT-207 was the highest. In mice treated with JPJDF, the expression intensity of FAK was higher in the normal tissue and lower in the cancer tissue than those of the other treatment groups. CONCLUSION: The mechanism accounting for the prolonged survival of HCC-bearing mice treated with JPJDF might be related to the reduction in weight loss and the benign regulation of PTEN, PI3K, and FAK. | Baoguo Sun Jun Meng Ting Xiang Zexiong Chen Yulong Li Lisha Lu Shijun Zhang Xiaolin Chen | 2013 | Journal of Traditional Chinese Medicine2013，33，4： | 12 |
| 4 | Diagnostic value of 5 serum biomarkers for hepatocellular carcinoma with different epidemiological backgrounds:A large-scale,retrospective study显示文摘Objective:Hepatocellular carcinoma(HCC)is a lethal global disease that requires an accurate diagnosis.We assessed the potential of 5 serum biomarkers(AFP,AFU,GGT-II,GPC3,and HGF)in the diagnosis of HCC.Methods:In this retrospective study,we measured the serum levels of each biomarker using ELISAs in 921 participants,including 298 patients with HCC,154 patients with chronic hepatitis(CH),122 patients with liver cirrhosis(LC),and 347 healthy controls from 3 hospitals.Patients negative for hepatitis B surface antigen and hepatitis C antibody(called'NBNC-HCC')and patients positive for the above indices(called'HBV-HCC and HCV-HCC')were enrolled.The selected diagnostic model was constructed using a training cohort(n=468),and a validation cohort(n=453)was used to validate our results.Receiver operating characteristic analysis was used to evaluate the diagnostic accuracy.Results:Theα-L-fucosidase(AFU)/α-fetoprotein(AFP)combination was best able to distinguish NBNC-HCC[area under the curve:0.986(95%confidence interval:0.958–0.997),sensitivity:92.6%,specificity:98.9%]from healthy controls in the test cohort.For screening populations at risk of developing HCC(CH and LC),the AFP/AFU combination improved the diagnostic specificity for early-stage HCC[area under the curve:0.776(0.712–0.831),sensitivity:52.5%,specificity:91.6%in the test group].In all-stage HBV-HCC and HCV-HCC,AFU was also the best candidate biomarker combined with AFP[area under the curve:0.835(0.784–0.877),sensitivity 69.1%,specificity:87.4%in the test group].All results were verified in the validation group.Conclusions:The AFP/AFU combination could be used to identify NBNC-HCC from healthy controls and hepatitis-related HCC from at-risk patients. | Dongming Liu Yi Luo Lu Chen Liwei Chen Duo Zuo Yueguo Li Xiaofang Zhang Jing Wu Qing Xi Guangtao Li Lisha Qi Xiaofen Yue Xiehua Zhang Zhuoyu Sun Ning Zhang Tianqiang Song Wei Lu Hua Guo | 2021 | Cancer Biology & Medicine2021，18，1： | 10 |
| 5 | MSX2 mediates entry of human pluripotent stem cells into mesendoderm by simultaneously suppressing SOX2 and activating NODAL signaling显示文摘发信号的 BMP 怎么集成到并且使动摇人的 pluripotent 干细胞(hPSCs ) 的 pluripotency 电路开始区别进单个细菌层是一个长期的难题。这里,我们报导肌肉片断 homeobox 2 (MSX2 ) , msh 家庭的一个 homeobox 抄写因素, BMP 发信号的直接目标基因和 hPSC 到 mesendoderm 的区别的一个主人调停人。MSX2 的强制表示足够废除 pluripotency 并且导致 hPSCs 的指导 mesendoderm 区别,当 MSX2 弄空损害 mesendoderm 正式就职时。MSX2 是在 hPSCs 的 BMP 小径的直接目标基因,并且能被 Wnt 信号 synergistically 在 mesendoderm 正式就职期间经由 LEF1 激活。而且, MSX2 使动摇通过到 SOX2 倡导者和 SOX2 抄写的压抑的直接绑定的 pluripotency 电路,当 MSX2 通过节的倡导者的直接绑定和激活由 SOX2 的同时的抑制和节的表示的正式就职控制 mesendoderm 系承诺时。有趣地, SOX2 能支持 MSX2 蛋白质的降级,建议在在干细胞命运的控制的二个指定系的因素之间的相互的对抗。一起,我们的调查结果揭示使动摇的关键新机制在 hPSCs 的 pluripotency 和指导系承诺。 | Qingqing Wu Leisheng Zhang Pei Su Xiaohua Lei Xin Liu Hongtao Wang Lisha Lu Yang Bai Tao Xiong Dong Li Zhengmao Zhu Enkui Duan Erlie Jiang Sizhou Feng Mingzhe Hang Yuanfu Xu Fei Wang Jiaxi Zhou | 2015 | Cell Research2015，25，12： | 8 |
| 6 | The role of bone marrow-derived cells in the origin of liver cancer revealed by single-cell sequencing显示文摘Objective:Epithelial cancers often originate from progenitor cells,while the origin of hepatocellular carcinoma(HCC)is still controversial.HCC,one of the deadliest cancers,is closely linked with liver injuries and chronic inflammation,which trigger massive infiltration of bone marrow-derived cells(BMDCs)during liver repair.Methods:To address the possible roles of BMDCs in HCC origination,we established a diethylnitrosamine(DEN)-induced HCC model in bone marrow transplanted mice.Immunohistochemistry and frozen tissue immunofluorescence were used to verify DENinduced HCC in the pathology of the disease.The cellular origin of DEN-induced HCC was further studied by single cell sequencing,single-cell nested PCR,and immunofluorescence-fluorescence in situ hybridization.Results:Studies by using single cell sequencing and biochemical analysis revealed that HCC cells in these mice were coming from donor mice BMDCs,and not from recipient mice.Furthermore,the copy numbers of mouse orthologs of several HCC-related genes previously reported in human HCC were also altered in our mouse model.DEN-induced HCCs exhibited a similar histological phenotype and genomic profile as human HCCs.Conclusions:These results suggested that BMDCs are an important origin of HCC,which provide important clues to HCC prevention,detection,and treatments. | Lu Chen Xianfu Yi Piao Guo Hua Guo Ziye Chen Chunyu Hou Lisha Qi Yongrong Wang Chengwen Li Peng Liu Yucun Liu Yuanfu Xu Ning Zhang | 2020 | Cancer Biology & Medicine2020，17，1： | 4 |
| 7 | Laser-based micro/nanofabrication in one, two and three dimensions显示文摘有各种各样的尺寸的功能的材料和结构的先进 micro/nanofabrication 在现代 nanoscience 和技术代表一个关键研究话题并且为象 nanoelectronics, nanophotonics 和 micro/nanoelectromechanical 系统那样的众多的出现技术变得极其重要。这评论系统地探索在制作挑战性经由常规途径被制作的各种各样的尺寸的 nanomaterials 和 micro/nanostructures 处理途径的非常规的材料。研究努力为生长和制造集中于基于激光的技术一个维(1D ) ,二维(2D ) 并且三维(3D ) nanomaterials 和 micro/nanostructures。下列研究话题被论及,包括:1 ) 为碳 nanotubes (CNT ) 的 1D nanomaterial 的高度有效的生长和集成的帮助激光的化学蒸汽免职(CVD ) , 2 ) 在周围的条件下面的 graphene 带子的直接写的激光(LDW ) ,并且 3 ) 经由添加剂和减少性的过程的 3D micro/nanostructures 的 LDW。与常规制造方法作比较,基于激光的方法在先进功能的材料和结构的 micro/nanofabrication 展出几个唯一的优点。为 1D CNT 生长,帮助激光的 CVD 进程能由于高度强烈的精力交货和导致激光的光近地的效果认识到两快速的材料合成和生长地点的紧密的控制和 CNT 的取向。为 2D graphene 合成和 patterning,房间温度并且露天绝缘的表面上的大规模 graphene 模式的制造被一个 LDW 过程成功地认识到。为 3D micro/nanofabrication,二光子的聚合(TPP ) 和减少性的多光子脱离(MPA ) 处理的添加剂的联合启用为常规制造方法挑战性的任意的复杂 3D micro/nanostructures 的制造。就基于激光的技术的众多的唯一的优点而言,基于激光的 micro/nanofabrication 被期望在先进功能的 micro/nano-devices 的制造起一个越来越重要的作用。 | Wei XIONG Yunshen ZHOU Wenjia HOU Lijia JIANG Masoud MAHJOURI-SAMANI Jongbok PARK Xiangnan HE Yang GAO Lisha FAN Tommaso BALDACCHINI Jean-Francois SILVAIN Yongfeng LU | 2015 | Frontiers of Optoelectronics2015，8，4： | 4 |
| 8 | Pyridinic nitrogen enriched porous carbon derived from bimetal organic frameworks for high capacity zinc ion hybrid capacitors with remarkable rate capability显示文摘Aqueous zinc ion hybrid capacitors(ZIHCs)hold great potential for large-scale energy storage applications owing to their high safety and low cost,but suffer from low capacity and energy density.Herein,pyridinic nitrogen enriched porous carbon(nPC)was successfully synthesized via the growth,subsequent annealing and acid etching of bimetal organic frameworks for high capacity and safe ZIHCs with exceptional rate capability.Benefiting from the mesopores for easy ion diffusion,high electrical conductivity enabled by in-situ grown carbon nanotubes matrix and residual metal Co nanoparticles for fast electron transfer,sufficient micropores and high N content(8.9 at%)with dominated pyridinic N(54%)for enhanced zinc ion storage,the resulting nPC cathodes for ZIHCs achieved high capacities of 302 and137 m Ah g^(-1) at 1 and 18 A g^(-1),outperforming most reported carbon based cathodes.Theoretical results further disclosed that pyridinic N possessed larger binding energy of-4.99 eV to chemically coordinate with Zn2+than other N species.Moreover,quasi-solid-state ZIHCs with gelatin based gel electrolytes exhibited high energy density of 157.6 Wh kg^(-1) at 0.69 kW kg^(-1),high safety and mechanical flexibility to withstand mechanical deformation and drilling.This strategy of developing pyridinic nitrogen enriched porous carbon will pave a new avenue to construct safe ZIHCs with high energy densities. | Yao Li Pengfei Lu Ping Shang Lisha Wu Xiao Wang Yanfeng Dong Ronghuan He Zhong-Shuai Wu | 2021 | Journal of Energy Chemistry2021，30，5： | 3 |
| 9 | Cytosolic phospholipase A2α modulates cell-matrix adhesion via the FAK/paxillin pathway in hepatocellular carcinoma显示文摘Objective: To explore the effect of cytosolic phospholipase A2α(cPLA2α) on hepatocellular carcinoma(HCC) cell adhesion and the underlying mechanisms.Methods: Cell adhesion, detachment, and hanging-drop assays were utilized to examine the effect of cPLA2α on the cell-matrix and cell-cell adhesion. Downstream substrates and effectors of cPLA2α were screened via a phospho-antibody microarray.Associated signaling pathways were identified by the functional annotation tool DAVID. Candidate proteins were verified using Western blot and colocalization was investigated via immunofluorescence. Western blot and immunohistochemistry were used to detect protein expression in HCC tissues. Prognosis evaluation was conducted using Kaplan-Meier and Cox-proportional hazards regression analyses.Results: Our findings showed that cPLA2α knockdown decreases cell-matrix adhesion but increases cell-cell adhesion in HepG2 cells. Microarray analysis revealed that phosphorylation of multiple proteins at specific sites were regulated by cPLA2α. These phosphorylated proteins were involved in various biological processes. In addition, our results indicated that the focal adhesion pathway was highly enriched in the cPLA2α-relevant signaling pathway. Furthermore, cPLA2α was found to elevate phosphorylation levels of FAK and paxillin, two crucial components of focal adhesion. Moreover, localization of p-FAK to focal adhesions in the plasma membrane was significantly reduced with the downregulation of cPLA2α. Clinically, cPLA2α expression was positively correlated with p-FAK levels. Additionally, high expression of both cPLA2α and p-FAK predicted the worst prognoses for HCC patients.Conclusions: Our study indicated that cPLA2α may promote cell-matrix adhesion via the FAK/paxillin pathway, which partly explains the malignant cPLA2α phenotype seen in HCC. | Piao Guo Yuchao He Lu Chen Lisha Qi Dongming Liu Ziye Chen Manyu Xiao Liwei Chen Yi Luo Ning Zhang Hua Guo | 2019 | Cancer Biology & Medicine2019，16，2： | 2 |
| 10 | Improved Electrochemical Performance Based on Nanostructured SnS_2@CoS_2–rGO Composite Anode for Sodium-Ion Batteries显示文摘A promising anode material composed of SnS_2@CoS_2 flower-like spheres assembled from SnS_2 nanosheets and CoS_2 nanoparticles accompanied by reduced graphene oxide(rGO) was fabricated by a facile hydrothermal pathway. The presence of rGO and the combined merits of SnS_2 and CoS_2 endow the SnS_2@-CoS_2–rGO composite with high conductivity pathways and channels for electrons and with excellent properties as an anode material for sodium-ion batteries(SIBs). A high capacity of 514.0 mAh g^(-1) at a current density of200 m A g^(-1) after 100 cycles and a good rate capability can be delivered. The defined structure and good sodium-storage performance of the SnS_2@CoS_2–rGO composite demonstrate its promising application in high-performance SIBs. | Xia Wang Xueying Li Qiang Li Hongsen Li Jie Xu Hong Wang Guoxia Zhao Lisha Lu Xiaoyu Lin Hongliang Li Shandong Li | 2018 | Nano-Micro Letters2018，10，3： | 2 |
| 11 | Improvement in the risk assessment of oral leukoplakia through morphology-related copy number analysis显示文摘Oral leukoplakia is the most common type of oral potentially malignant disorders and considered a precursor lesion to oral squamous cell carcinoma.However,a predictor of oral leukoplakia prognosis has not yet been identified.We investigated whether copy number alteration patterns may effectively predict the prognostic outcomes of oral leukoplakia using routinely processed paraffin sections.Comparison of copy number alteration patterns between oral leukoplakia with hyperplasia(HOL,n=22)and dysplasia(DOL,n=21)showed that oral leukoplakia with dysplasia had a higher copy number alteration rate(86%)than oral leukoplakia with hyperplasia(46%).Oral leukoplakia with dysplasia exhibited a wider range of genomic variations across all chromosomes compared with oral leukoplakia with hyperplasia.We also examined a retrospective cohort of 477 patients with oral leukoplakia with hyperplasia with detailed follow-up information.The malignant transformation(MT,n=19)and leukoplakia recurrence(LR,n=253)groups had higher frequencies of aneuploidy events and copy number loss rate than the free of disease(FD,n=205)group.Together,our results revealed the association between the degree of copy number alterations and the histological grade of oral leukoplakia and demonstrated that copy number alteration may be effective for prognosis prediction in oral leukoplakia patients with hyperplasia. | Xiaotian Li Lu Liu Jianyun Zhang Ming Ma Lisha Sun Xuefen Li Heyu Zhang Jianbin Wang Yanyi Huang Tiejun Li | 2021 | Science China(Life Sciences)2021，64，9： | 2 |
| 12 | Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening显示文摘The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ripening.However,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely unknown.Here,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening stage.Consistent with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the ripening.DNA affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB AM1.Stably overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ripening.Moreover,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation activity.Importantly,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by MaMsrA4.Collectively,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9. | Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang | 2023 | Journal of Integrative Plant Biology2023，65，1： | 1 |
| 13 | A new and informative active learning approach for support vector machine显示文摘 | Lisha Hu Shuxia Lu Xizhao Wang | 2013 | Information Sciences2013，，： | 1 |
| 14 | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study显示文摘 | Lu Li Shengzheng Wu Zheng Liu Zhongxiong Zhuo Kaibin Tan Hongmei Xia Lisha Zhuo Xiaojun Deng Yunhua Gao Yali Xu Kishore K. Wary | 2015 | Stem Cells International2015，，： | 1 |
| 15 | The β-alanyl-monoamine synthase ebony is regulated by schizophrenia susceptibility gene dysbindin in Drosophila显示文摘The Drosophila homolog of schizophrenia susceptibility gene dysbindin(Ddysb)affects a range of behaviors through regulation of multiple neurotransmitter signals,including dopamine activity.To gain insights into mechanisms underlying Ddysb-dependent regulation of dopamine signal,we investigated interaction between Ddysb and Ebony,the Drosophilaβ-alanyl-monoamine synthase involved in dopamine recycling.We found that Ddysb was capable of regulating expression of Ebony in a bi-directional manner and its subcellular distribution.Such regulation is confined to glial cells.The expression level of ebony and its accumulation in glial soma depend positively on Ddysb activity,whereas its distribution in glial processes is bound to be reduced in response to any alterations of Ddysb from the normal control level,either an increase or decrease.An optimal binding ratio between Dysb and Ebony might contribute to such non-linear effects.Thus,Ddysb-dependent regulation of Ebony could be one of the mechanisms that mediate dopamine signal. | LU BinYan SHAO LiSha FENG ShanXi WANG Tian ZHONG Yi | 2014 | Science China(Life Sciences)2014，57，1： | 1 |
| 16 | Healing Effect and Underlying Mechanism of Silver Nanoparticles Prepared Using Carboxymethyl Chitosan under Solar Irradiation显示文摘In our previous study,silver nanoparticles were prepared using AgNO_(3) and carboxymethyl chitosan(CMCS)which is commercially available with solar irradiation.In this study,the efficacy and safety of silver nanoparticles prepared by this method were evaluated for healing wounds in rats with diabetes.We also attempted to determine the underlying mechanism and influencing factors of the silver nanomaterial preparation method.Compared with growth factors,silver nanoparticles exhibit better healing effects for rats with diabetes.No remnant silver ions were detected in the major organs of these rats after the application of silver nanoparticles.Silver nanoparticles prepared using CMCS are less toxic than those prepared from the conventional method,promote the proliferation of skin fibroblasts,and are promising as a topical medication for infected wounds.An obvious breakage process of the particles is observed during the growth of nanocrystalline silver in CMCS.In this study,we also attempted to determine whether this method is suitable for synthesizing silver nanoparticles using N-trimethyl chitosan chloride and sodium alginate were used in this particular experiment.The characteristic UV-vis absorbance peak of silver nanoparticles was found only in the reaction mixture containing N-trimethyl chitosan chloride.Our study demonstrates that free radicals are the key factor in this silver nanoparticle preparation method. | Yi Long Chunling Zhang Lan Chen Tietao Di Lu Chen Lisha Tang Kaizhong Luo Yu Yan Pingzhen Tong Jie Xiang | 2021 | Journal of Renewable Materials2021，9，6： | 0 |
| 17 | Induction of diabetes in cynomolgus monkey with one shot of analytical grade streptozotocin显示文摘Backgrounds:Streptozotocin(STZ)-induced diabetic monkey is a wide used preclinical animal model for the investigation of diabetes such as islet transplantation and development of diabetic drugs.There are serious side effects of this method,including nausea,emesis,weight loss,liver damage,renal failure,and metabolic acidosis.In order to reduce the side effects,diabetic monkeys were induced using clinicalgrade STZ.However,clinical-grade STZ is not available in China.Here,we establised a method by using 100 mg/kg analytical-grade STZ to induce complete diabetes in cynomolgus monkey without generating adverse effects to liver and renal.Methods:Three cynomolgus monkeys were used in this study.100 mg/kg STZ dissolved in normal saline and infused through vein line in 5 minutes after indwelling catheter in the carotid artery and jugular vein.After the STZ administration,blood glucose levels were examined every 1 or 2 hours in the first 48 hours.Then,blood glucose levels were examined twice per day during the first week after the STZ injection.Insulin and C-peptide levels were measured by ELISA.Blood chemistry of hepatic and renal function tests were performed.Insulin and glucagon expression in the islet of diabetic monkey and normal monkey were examined by immunohistochemistry assays.Results:The stimulated C-peptide level(Intravenous glucose tolerance test)which is less than 0.5 ng/mL,the triphasic blood glucose response and the destroyed β cell suggested the complete induction of diabetes model.No apparent adverse effects were observed including no signs of vomiting and toxicity after STZ injection.Conclusion:In summary,we established a safe and reproducible STZ-induced diabetic cynomolgus monkey model for islet transplantation which will be used to develop novel approaches for the treatment of diabetes. | Zhengzhao Liu Ying Lu Wenbao Hu Hidetaka Hara Yifan Dai Zhiming Cai Lisha Mou | 2020 | Animal Models and Experimental Medicine2020，3，1： | 0 |
| 18 | Heterogeneity of neutrophils in cancer:one size does not fit all显示文摘Neutrophils play an essential role in the defense against bacterial infections and orchestrate both the innate and adaptive immune responses.With their abundant numbers,diverse function and short life span,these cells are at the forefront of immune responses,and have gained attention in recent years because of their presence in tumor sites.Neutrophil involvement pertains to tumor cells'ability to construct a suitable tumor microenvironment(TME)that accelerates their own growth and malignancy,by facilitating their interaction with surrounding cells through the circulatory and lymphatic systems,thereby influencing tumor development and progression.Studies have indicated both pro-and anti-tumor properties of infiltrating neutrophils.The TME can exploit neutrophil function,recruitment,and even production,thus resulting in pro-tumor properties of neutrophils,including promotion of genetic instability,tumor cell proliferation,angiogenesis and suppression of anti-tumor or inflammatory response.In contrast,neutrophils can mediate anti-tumor resistance by direct cytotoxicity to the tumor cells or by facilitating anti-tumor functions via crosstalk with T cells.Here,we summarize current knowledge regarding the effects of neutrophil heterogeneity under homeostatic and tumor conditions,including neutrophil phenotype and function,in cancer biology. | Song Chen Qingyu Zhang Lisha Lu Chunhui Xu Jiajia Li Jiali Zha Fengxia Ma Hongbo R.Luo Alan Y.Hsu | 2022 | Cancer Biology & Medicine2022，19，12： | 0 |
| 19 | Nucleoporin 160 Regulates Flowering through Anchoring HOS1 for Destabilizing CO in Arabidopsis显示文摘Nuclear pore complexes(NPCs),which comprise multiple copies of nucleoporins(Nups),are large protein assemblies embedded in the nuclear envelope connecting the nucleus and cytoplasm.Although it has been known that Nups affect flowering in Arabidopsis,the underlying mechanisms are poorly understood.Here,we show that loss of function of Nucleoporin 160(Nup160)leads to increased abundance of CONSTANS(CO)protein and the resulting upregulation of FLOWERING LOCUS T(FT)specifically in the morning.We demonstrate that Nup160 regulates CO protein stability through affecting NPC localization of an E3-ubiquitin ligase,HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1(HOS1),which destabilizes CO protein in the morning period.Taken together,these results provide a mechanistic understanding of Nup function in the transition from vegetative to reproductive growth,suggesting that deposition of HOS1 at NPCs by Nup160 is essential for preventing precocious flowering in response to photoperiod in Arabidopsis. | Chunying Li Lu Liu Zhi Wei Norman Teo Lisha Shen Hao Yu | 2020 | Plant Communications2020，1，2： | 0 |
| 20 | Dry passivation of austenitic SUS 301L stainless steel against pitting corrosion in marine atmospheric environment显示文摘Being an exclusive construction material for lightweight rail vehicles,protection from pitting corrosion in harsh marine atmospheric environment in high humidity and Cl-ion concentration is critical for austenitic SUS 301L stainless steel(SS),especially when it inevitably suff ers from mechanical damages during post disposals.Herein,an innovative dry passivation method for austenitic SUS 301L SS was established in a closed air atmosphere at low temperature and constant pressure.The process parameters were optimized,and the passivation mechanism was explained using polarization curve,electrochemical impedance spectroscopy(EIS),X-ray photoelectron spectroscopy(XPS),and contact angle measurement.The pitting corrosion susceptibility of the passive fi lm prepared in a closed air chamber under 1.0×10^(5) Pa at 80℃ for 80 min was evaluated in 3.5%NaCl solution and exhibited higher pitting potential and corrosion resistance,lower passivity-maintaining current density,and wettability when compared with conventional nitric acid treatment.Besides,dry passivation facilitated the repairing of the surface structural defect itself and the post-processing damage,similar to the accelerated aging of fi lm.The decrease in oxygen concentration and convection-diff usion strengthened the preferential chromium oxidation to form a compact chromium-rich passive fi lm to resist the aggression of Cl-ion. | Yonghong LU Xiao LIU Lisha WANG Jinghong YANG Haibo XU | 2022 | Journal of Oceanology and Limnology2022，40，4： | 0 |
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