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| 1 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://www.hi-tom.net/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 2 | Chitosan conduits combined with nerve growth factor microspheres repair facial nerve defects显示文摘Microspheres containing nerve growth factor for sustained release were prepared by a compound method,and implanted into chitosan conduits to repair 10-mm defects on the right buccal branches of the facial nerve in rabbits.In addition,chitosan conduits combined with nerve growth factor or normal saline,as well as autologous nerve,were used as controls.At 90 days post-surgery,the muscular atrophy on the right upper lip was more evident in the nerve growth factor and normal saline groups than in the nerve growth factor-microspheres and autologous nerve groups.Electrophysiological analysis revealed that the nerve conduction velocity and amplitude were significantly higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups.Moreover,histological observation illustrated that the diameter,number,alignment and myelin sheath thickness of myelinated nerves derived from rabbits were higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups.These findings indicate that chitosan nerve conduits combined with microspheres for sustained release of nerve growth factor can significantly improve facial nerve defect repair in rabbits. | Huawei Liu Weisheng Wen Min Hu Wenting Bi Lijie Chen Sanxia Liu Peng Chen Xinying Tan | 2013 | Neural Regeneration Research2013，8，33： | 22 |
| 3 | The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance显示文摘真核细胞的有机体有允许错误褶层或 unassembled 蛋白质在 endoplasmic 蜂窝胃被保留的质量控制的机制(嗯) 并且随后由嗯联系降级(ERAD ) 降级了。ERAD 小径很好在酵母和哺乳动物被学习;然而,植物 ERAD 的生物功能没被报导。通过分子、细胞的生物途径,我们发现 ERAD 是必要的让植物克服盐应力。在蛋白质在植物房间增加了的盐处理 ubiquitinated 之上,特别展开了快速在 ER 积累了并且随后导致了的蛋白质嗯压力回答。在 ERAD 小径的 HRD1/HRD3 建筑群的 HRD3A 的缺点导致了展开的蛋白质反应(UPR ) 的改变,腌的增加的植物敏感,和在植物房间的 ERAD 底层的保留。而且,我们证明从 ER 的那个 Ca 2+ 版本涉及 UPR 的举起,反应的氧种类(ROS ) 参与 ERAD 相关的植物盐反应小径。 | Lijing Liu Feng Cui Qingliang Li Bojiao Yin Huawei Zhang Baoying Lin Zaorong Wu Ran Xia Sanyuan Tang Qi Xie | 2011 | Cell Research2011，21，6： | 19 |
| 4 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 5 | Myelin protein zero and its antibody in serum as biomarkers of n-hexane-induced peripheral neuropathy and neurotoxicity effects显示文摘 | Jia Xiaowei Liu Qingjun Zhang Yanshu Dai Yufei Duan Huawei Bin Ping Niu Yong Liu Jie Zhong Liuzhen Guo Jisheng Liu Xiaofeng Zheng Yuxin | 2014 | Chinese Medical Journal2014，，8： | 8 |
| 6 | Uncompromised NK cell activation is essential for virus-specific CTL activity during acute influenza virus infection显示文摘Natural killer (NK) cells are indispensable components of both the innate and adaptive immune response. However,their precise roles in the cross-talk between innate and adaptive immunity during influenza virus infection remaincontroversial. By comparing NK cell dynamics and activity under a sub-lethal dose and high dose of influenza virusinfection, we showed that influenza virus PR8 directly infected NK cells during natural infection, which wasconsistent with our previous findings obtained from an in vitro investigation of human NK cells. The impairments incytotoxicity and IFN-γ production by spleen NK cells following high-dose infection were accompanied by decreasedvirus-specific killing mediated by cytotoxic T lymphocytes (CTLs). Importantly, the weakened CTL activity could bereversed by adoptive transfer of spleen NK cells harvested from low-dose-infected mice but not healthy donors.Taken together, our data provide direct evidence supporting the contribution of NK cells to antiviral T-cellresponses. This study also indicates that a novel NK-targeted immune evasion strategy is used by influenza virusto shrink both innate and adaptive immune responses. | Yuan Liu Jian Zheng Yinping Liu Liyan Wen Lei Huang Zheng Xiang Kwok-Tai Lam Aizhen Lv Huawei Mao Yu-Lung Lau Wenwei Tu | 2018 | Cellular & Molecular Immunology2018，15，9： | 6 |
| 7 | Study on the Technology of Ni-P Electroless Plating on the Mg Alloy显示文摘Base on the analysis for the corrosion mechanism of the Mg alloys,the technology of Ni-P directly electroless plating on the Mg-10Li-1Zn alloys was studied.The effects of the pre-treatment on the morphologies of substrate and plating films were investigated,The result shows that Ni-P electroless plating could be divided two steps:the induction period and the rapid deposition period.The Ni-P film is amorphous structure:A excellent Ni-P coating can be obtained under the optimal parameter.The Ni-P coating can obviously improve the corrosion resistance of Mg alloy in variety environment. | Liu Yihan Sun Huawei Jiang Yanan | 2012 | 稀有金属材料与工程2012，41，S1： | 5 |
| 8 | Bioinspired Unidirectional Liquid Transport Micro-nano Structures:A Review显示文摘Unidirectional liquid transport without any need of external energy has drawn worldwide attention for its potential applications in various fields such as microfluidics,biomedicine and mechanical engineering.In nature,numerous creatures have evolved such extraordinary unidirectional liquid transport ability such as spider sik,Sarracenia's trichomes,and Nepenthes alata's peristome,etc.This review summarizes the current progresses of natural unidirectional liquid transport on 1-Dimensional(1D)linear structure and 2-Dimensional(2D)surface stucture.The driving force of unidirectional liquid transport which is determined by unique structure exist distinct differences in physics.The fundamental understanding of 1D and 2D unidirectionaliquid transport especially about hierarchical structural characteristics and their transport mechanism were concentrated,and various bioinspired fabrication methods are also introduced.The applications of bioinspired directional liquid transport are demonstrated especially in fields of microfluidies,biomedical devices and anti-icing surfaces.With newly developed smart materials,various liquid transport regulation strategies are also summarized for the control of transport speed,direction guiding,etc.Finally,we provide new insights and future perspectives of the directional transport materials. | Liwen Zhang Guang Liu Huawei Chen Xiaolin Liu Tong Ran Yi Zhang Yang Gan Deyuan Zhang | 2021 | Journal of Bionic Engineering2021，18，1： | 4 |
| 9 | Avian influenza virus directly infects human natural killer cells and inhibits cell activity显示文摘Natural killer(NK) cell is a key component of innate immunity and plays an important role in host defense against virus infection by directly destroying infected cells. Influenza is a respiratory disease transmitted in the early phase of virus infection. Evasion of host innate immunity including NK cells is critical for the virus to expand and establish a successful acute infection. Previously,we showed that human influenza H1N1 virus infects NK cells and induces cell apoptosis, as well as inhibits NK cell activity. In this study, we further demonstrated that avian influenza virus also directly targeted NK cells as an immunoevasion strategy. The avian virus infected human NK cells and induced cell apoptosis. In addition, avian influenza virion and HA protein inhibited NK cell cytotoxicity. This novel strategy has obvious advantages for avian influenza virus, allowing the virus sufficient time to expand and subsequent spread before the onset of the specific immune response. Our findings provide an important clue for the immunopathogenesis of avian influenza,and also suggest that direct targeting NK cells may be a common strategy used by both human and avian influenza viruses to evade NK cell immunity. | Huawei Mao Yinping Liu Sin Fun Sia JS Malik Peiris Yu-Lung Lau Wenwei Tu | 2017 | Virologica Sinica2017，32，2： | 4 |
| 10 | Nutrient resorption strategies of three oak tree species in response to interannual climate variability显示文摘Background:Nutrient resorption is critical for plants toward balancing their nutritional requirements and adapting to environmental variabilities,which further impacts litter quality and nutrient cycling.However,the interannual variability of nutrient resorption under climate change remains unclear.Methods:We investigated the five-year nutrient resorption efficiencies(NuRE,%)of 14 elements in three deciduous oak tree species(Quercus aliena var.acuteserrata,Q.glandulifera,and Q.variabilis)in a warm-temperate forest of Central China and assessed their relationships with interannual climate and soil factors.Results:Nutrient resorption did not differ between species but varied significantly between different years.For each year,N,P,S,K,C,Mg,and Zn were preferentially resorbed in all of the oak species in contrast to Ca,Na,Mn,Ba,Al,Fe,Cu,which were to some extent discriminated.Among the 14 elements,the NuRE of C,N,P,S,Ca,and Mg was more sensitive to interannual climate variations in the three oak species.The carbon resorption efficiency was significantly increased during the driest year of the study(2014);N resorption efficiency was reduced with temperature;whereas N and P resorption efficiency initially decreased and then increased with precipitation.Moreover,the elements with higher NuREs typically had lower coefficient of variation(CV)in all three oak species.Conclusions:Different oak species exhibited analogous nutrient conservation strategies in response to annual climate variabilities,and interannual climate variations strongly impacted plant nutrient resorption.Deciduous plants may establish a tradeoff mechanism to rebalance somatic nutrients for regrowth at the end of the growing season. | Baoming Du Huawei Ji Shirong Liu Hongzhang Kang Shan Yin Chunjiang Liu | 2021 | Forest Ecosystems2021，8，4： | 4 |
| 11 | Robust reliable guaranteed cost control for uncertain singular systems with time-delay显示文摘The robust reliable guaranteed cost control for uncertain singular delay systems with actuator failures and a given quadratic cost function is studied. The system under consideration involves constant time-delay and norm-bounded parameter uncertainties. The purpose is to design state feedback controllers which can tolerate actuator failure, such that the closed-loop system is stable, and the specified cost function has an upper bound for all admissible uncertainties. The sufficient conditions for the solvability of this problem are obtained by a linear matrix inequality (LMI) method. Furthermore, a numerical example is given to demonstrate the applicability of the proposed approach. | Qixun Lan Yuxiao Liu Huawei Niu Jiarong Liang | 2010 | Journal of Systems Engineering and Electronics2010，21，1： | 4 |
| 12 | Effect of ultrasonic vibration on the dechromisation corrosion of a CuCr alloy in HCl solution显示文摘The effect of ultrasonic vibration on the dechromisation corrosion of a CuCr alloy in HCl solution was studied and the corrosion mechanisms were analyzed. It is found that ultrasonic vibration reduces the dechromisation incubation time, accelerates the dechromisation corrosion rate, decreases the temperature and concentration of HCl solution, and when the dechromisation occurs it seriously weakens the microstructure of dechromisation layer. It is concluded that ultrasonic vibration can accelerate destruction of the passivation film on the Cr surface and increase the activities of Cl· and Cr. | XU Tao GAO Huawei LIU Jianhua YU Cuiyan | 2007 | Rare Metals2007，26，4： | 4 |
| 13 | Evolution of Pore Size Distribution and Mean Pore Size in Lotus-type Porous Magnesium Fabricated with Gasar Process显示文摘吝啬的毛孔上的压力缩放的气体的效果,孔和毛孔缩放莲花类型的分发与 Gasar 过程制作的多孔的镁被调查。理论分析和试验性的结果都显示那在那里存在到为与更狭窄的毛孔尺寸分发和更小的毛孔尺寸生产 lotus-typestructures 的氩 p_(Ar ) 的氢 p_(H_2 ) 的部分压力的最佳的比率。毛孔尺寸分发和毛孔尺寸上的团结模式的效果也被讨论。 | Yuan LIU Yanxiang LI Huawei ZHANG Jiang WAN | 2006 | Journal of Materials Science & Technology2006，22，3： | 3 |
| 14 | Springback characteristics in U-channel forming of tailor rolled blank显示文摘Residual stress developed during the rolling process of tailor rolled blank (TRB) can affect the springback of finished parts considerably. Springback characteristics of unannealed and annealed TRBs were investigated by means of numerical simulation and experiments taking U-Channel as an example. TRBs were annealed by the annealing process (700 ℃, holding time 10 h), then stamping and springback processes of unannealed and annealed TRBs were simulated, and corresponding experiments were also carried out. Effects of the transition zone length, the blank thickness, the friction coeffcient and the die clearance on the springback of TRB were analyzed. The results demonstrate that the springback of TRB annealed at 700 ℃ for 10 h reduces significantly. For unannealed and annealed U-Channels, the springback of TRB U-Channel is in direct proportion to the die clearance and is in inverse proportion to the transition zone length, the blank thickness and the friction coeffcient. Springbacks of the thinner monolithic (uniform thickness) blank, the thinner side of TRB, the thicker side of TRB and the thicker monolithic blank are sorted in descending order. | Huawei ZHANG Lizhong LIU Ping HU Xianghua LIU | 2012 | Acta Metallurgica Sinica(English Letters)2012，25，3： | 3 |
| 15 | Dual-channel type tunable field-effect transistors based on vertical bilayer WS2(1−x)Se2x/SnS2 heterostructures显示文摘Layered semiconductor heterostructures are essential elements in modern electronic and optoelectronic devices.Dynamically engineering the composition of these heterostructures may enable the flexible design of the properties of heterostructure-based electronics and optoelectronics as well as their optimization.Here,we report for the first time a two-step chemical vapor deposition approach for a series of WS2(1−x)Se2x/SnS2 vertical heterostructures with high-quality and large areas.The steady-state photoluminescence results exhibit an obvious composition-related quenching ratio,revealing a strong coherence between the band offset and the charge transfer efficiency at the junction interface.Based on the achieved heterostructures,dual-channel backgate field-effect transistors were successfully designed and exhibited typical composition-dependent transport behaviors,and pure n-type unipolar transistors to ambipolar transistors were realized in such systems.The direct vapor growth of these novel vertical WS2(1−x)Se2x/SnS2 heterostructures could offer an interesting system for probing new physical properties and provide a series of layered heterostructures for high-quality devices. | Biyuan Zheng Dong Li Chenguang Zhu Jianyue Lan Xingxia Sun Weihao Zheng Huawei Liu Xuehong Zhang Xiaoli Zhu Yexin Feng Tao Xu Litao Sun Gengzhao Xu Xiao Wang Chao Ma Anlian Pan | 2020 | InfoMat2020，2，4： | 3 |
| 16 | The Effect of 2,5-hexanedione on Myelin Protein Zero Expression,and Its Mitigation Using Ginkgo Biloba Extract显示文摘Objective To investigate the role of myelin protein zero (P 0) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury,and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.Methods After 4 weeks of treatment with 2,5-HD at different doses (50,100,200,400 mg/kg) in rats,changes in the levels of P 0 in rat sciatic nerves was investigated,and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.Results The blood-nerve barrier (BNB) permeability of the sciatic nerve increased,and the expression of P 0 mRNA and P 0 protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks.Pretreatment with Egb761 protected against BNB interruption,and inhibited P 0 mRNA and protein reduction during 2,5-HD treatment.Pretreatment with Egb761 significantly reduced loss of body weight (P<0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P<0.01).It also reduced the signs of neurotoxicity induced by 2,5-HD.Conclusion 2,5-HD inhibited the expression of P 0 in a dose-dependent manner,and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD.Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats. | ZHAO Lei LIU QingJun CHEN Hong DUAN HuaWei BIN Ping LIU Qing NIU Yong DAI YuFei ZHENG YuXin | 2011 | Biomedical and Environmental Sciences2011，24，4： | 3 |
| 17 | Ambient TSP concentration and dustfall variation in Urumqi,China显示文摘Total suspended particulate(TSP, particle diameter≤100 μm) was the dominant air pollutant and significantly influenced local air quality. In this paper, we investigated the interannual and seasonal variations of TSP and dustfall of the atmosphere over Urumqi, the capital city of Xinjiang Uygur autonomous region, northwestern China, basing on environmental monitoring records and meteorological data from 1986–2012. The results showed that during the study period, annual average TSP concentration decreased from 716 to 260 μg/m3, with an average level of 422.9 μg/m3, while dustfall intensity reduced from 350.4 to 166.6 t/(km2?a), with an average level of 259 t/(km2?a). Over 50% of the annual pollution days were induced mainly by TSP. Spring and winter had relatively higher dustfall intensities, and dense traffic and residential areas had the highest dustfall intensities in the Urumqi metropolitan area. With an annual average precipitation of less than 300 mm, atmospheric particulates in Urumqi could be hardly removed through wet deposition. During spring and summertime prevailing winds from northwest and northeast could carry aeolian dust particles from sandy deserts to Urumqi. Aeolian dusts from deserts would remain to be a priority regarding air pollution control in arid oasis cities. | XiaoXiao ZHANG Xi CHEN YuHong GUO ZiFa WANG LianYou LIU Cottle PAUL ShengYu LI HuaWei PI | 2014 | Journal of Arid Land2014，6，6： | 3 |
| 18 | QTL mapping of drought tolerance traits in soybean with SLAF sequencing显示文摘Drought stress is an important factor affecting soybean yield.Improving drought tolerance of soybean varieties can increase yield and yield stability when the stress occurs.Identifying QTL related to drought tolerance using molecular marker-assisted selection is able to facilitate the development of drought-tolerant soybean varieties.In this study,we used a high-yielding and drought-sensitive cultivar‘Zhonghuang 35’and a drought-tolerant cultivar‘Jindou 21’to establish F6:9 recombinant inbred lines.We constructed a highdensity genetic map using specific locus amplified fragment sequencing(SLAF-Seq)technology.The genetic map contained 8078 SLAF markers distributing across 20 soybean chromosomes with a total genetic distance of 3780.98 c M and an average genetic distance of0.59 c M between adjacent markers.Two treatments(irrigation and drought)were used in the field tests,the Additive-Inclusive Composite Interval Mapping(ICIM-ADD)was used to call QTL,and plant height and seed weight per plant were used as the indicators of drought tolerance.We identified a total of 23 QTL related to drought tolerance.Among them,seven QTL(q PH2,q PH6,q PH7,q PH17,q PH19-1,q PH19-2,and q PH19-3)on chromosomes 2,6,7,17,and 19 were related to plant height,and five QTL(q SWPP2,q SWPP6,q SWPP13,q SWPP17,and q SWPP19)on chromosomes 2,6,13,17,and 19 were related to seed weight and could be considered as the major QTL.In addition,three common QTL(q PH6/q SWPP6,q PH17/q SWPP17,and q PH19-3/q SWPP19)for both plant height and seed weight per plant were located in the same genomic regions on the same chromosomes.Three(q PH2,q PH17,and q PH19-2)and four novel QTL(q SWPP2,q SWPP13,q SWPP17,and q SWPP19)were identified for plant height and seed weight per plant,respectively.Two pairs of QTL(q PH2/q SWPP2 and q PH17/q SWPP17)were also common for both plant height and seed weight per plant.These QTL and closely linked SLAF markers could be used to accelerate breeding for drought tolerant cultivars via MAS. | Honglei Ren Jianan Han Xingrong Wang Bo Zhang Lili Yu Huawei Gao Huilong Hong Rujian Sun Yu Tian Xusheng Qi Zhangxiong Liu Xiaoxia Wu Li-Juan Qiu | 2020 | The Crop Journal2020，8，6： | 2 |
| 19 | Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice显示文摘In study,we aimed to determine the mechanisms underlying the gastroprotective effects of sodium copper chlorophyllin(SCC)against ethanol-induced gastric ulcer injury in mice.First,the gastroprotective effects of SCC against gastric ulcer induced by ethanol were assessed.Then,biochemical,histopathological,immunohistochemistry assays,and western blot analysis were conducted to determine the possible mechanisms of action underlying the effects of SCC.Compared to the effects of omeprazole(OME)in a confirmed mouse model of ethanol-induced gastric ulcer injury,treatment with various doses of SCC resulted in up-regulation of Bcl-2 and down-regulation of the pro-apoptotic protein Bax.Significant decreases in the levels of the malondialdehyde(MDA),myeloperoxidase(MPO),and NO in the gastric tissues were observed.Furthermore,inflammatory cytokine analysis revealed that SCC treatment inhibited the expressions of TNF-αand IL-6,greatly reduced the phosphorylation level of IκB,and repressed the nuclear translocation of NF-κB p65,which demonstrated that SCC inhibited the activation of the NF-κB pathway.The present findings suggest that the protective effects of SCC may be beneficial as a potential preventive and therapeutic agent for gastric ulcer through the NF-κB pathway.Taken together,SCC administration significantly decreased the levels of MPO,NO,and MDA in gastric tissue and exerted a powerful anti-inflammatory activity as demonstrated by reduction in the secretions of proinflammatory mediators such as IL-6 and TNF-α in the serum of mice exposed to ethanol. | Huawei Lv Yan Lin Peigang Liu Weiqing Liang Kemin Wei Jinbao Pu Hongjian Zhang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 2 |
| 20 | Metal-gas eutectic growth during unidirectional solidification显示文摘 | Yuan Liu Yanxiang Li Jiang Wan Huawei Zhang | 2006 | Metallurgical and Materials Transactions A2006，，9： | 2 |
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