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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Exosome-mediated transfer of miR-1290 promotes cell proliferation and invasion in gastric cancer via NKD1显示文摘Currently, exosomes rich in RNAs and proteins are regarded as vital mediators of intercellular communication. Here, we aimed to explore the effects of exosomal miR-1290 in gastric cancer (GC) and understand its mechanism of action on GC progression. We first isolated exosomes from serum samples of GC patients and healthy people and characterized them by transmission electron microscopy. Then, we examined the expression level of miR-1290 contained in the exosomes by quantitative reverse-transcription polymerase chain reaction and found that exosomal miR-1290 was overexpressed in GC patients and cell lines. Promotion of proliferation, migration, and invasiveness of GC cells was noted after they were incubated with the isolated miR-1290-rich exosomes compared with incubation with a negative control. Furthermore, we predicted that naked cuticle homolog 1 (NKD1) mRNA is a direct target of miR-1290 and confirmed their interaction by a dual luciferase reporter assay. NKD1 overexpression attenuated the stimulatory effects of miR-1290 on GC cells. Collectively, our results suggest that exosomal miR-1290 enhances GC cell proliferation and invasion by targeting NKD1 mRNA and downregulating NKD1 expression. A better understanding of this process may facilitate the development of novel therapeutic agents for GC. | Jiying Huang Manru Shen Meizhu Yan Ying Cui Zhenjun Gao Xin Meng | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 19 |
| 3 | The Occurrence of Oleananes in the Beibuwan Basin and Its Application to the Study of Maturity and Oil-Source Rock Correlation显示文摘摘要: oleanane 参数,即,在 Beibuwan 盆的二沉积的列的 OP (oleananes/( oleananes+C30hopanes )),华南海,能被用来限定油产生窗户的顶,与在井 M1 的 0.53 的 Ro (/%)并且 0.55 在井 H1/Hd1/Hd2 ,分别地。与 vitrinite 反射(Ro/%) 作比较, OP 展示一个动态范围和罐头更精确显示油产生窗户。由使用 OP 和另外的 geochemical 索引,油来源关联也被进行。它建议在井 M1 和 M2 的油在 situ 从来源岩石被导出。在 Huachang 的 mudstone 高举不是为在这个区域的油的主要来源岩石。OP 也是在一些第三级的湖的盆的一个有用油来源关联参数。 | LI Meijun WANG Tieguan LIU Ju ZHANG Meizhu LU Hong MA Qinglin GAO Lihui | 2008 | Acta Geologica Sinica(English Edition)2008，82，3： | 9 |
| 4 | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose显示文摘The speed of flower opening is closely related to their ornamental period.Ethylene functions as a negative regulator involved in the regulation of the petal expansion process.In this study,we isolated a NF-YC transcription factor gene,RhNF-YC9,fromrose petals.RhNF-YC9 expression was induced at the early stages of flower opening but was inhibited by ethylene treatment.Silencing RhNF-YC9 decreased the speed of petal expansion from stage 2 to stage 5.The expressions of 11 cell expansion-related genes involved in cell wall loosening,cell turgor modulation,and cytoskeleton remodeling were significantly down-regulated in RhNF-YC9-silenced petals.We also found that silencing RhNF-YC9 decreased the expression of gibberellin acid(GA)biosynthetic gene RhGA20ox while significantly increasing the transcripts of GA catabolic gene RhGA2ox,reducing the accumulation of GA4 and GA7.The influence of ethylene treatment on the expression of RhGA20ox and RhGA2ox showed the same trend.These results together suggested that RhNF-YC9 positively regulated the speed of petal expansion and mediated the crosstalk between ethylene and GA.Our findings revealed a new insight into the function of NF-YC transcription factors involved in ethylene-regulated petal expansion. | Changxi Chen Nisar Hussain Yaru Wang Mingtong Li Lin Liu Meizhu Qin Nan Ma Junping Gao Xiaoming Sun | 2020 | Horticultural Plant Journal2020，6，6： | 9 |
| 5 | The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes显示文摘 | Juan Chen Zhaokui Cai Meizhu Bai Xiaohua Yu Chao Zhang Changchang Cao Xihao Hu Lei Wang Ruibao Su Di Wang Yingpeng Yao Rong Ye Baidong Hou Yang Yu Shuyang Yu Jinsong Li Yuanchao xue | 2018 | Cell Research2018，28，10： | 4 |
| 6 | IMPROVEMENTS IN THE TEACHING OF LISTENING显示文摘In teaching listening the teacher’s attention should go to training the students for listening inreal life.Authentic listening material and sound teaching procedures are two key-features of a good listen-ing class. | Hu Meizhu Anqing Teachers’ College,Anhui | 1999 | Chinese Journal of Applied Linguistics1999，22，2： | 3 |
| 7 | 如何评阅学生作文(英文)显示文摘批改学生的作文费时又费力,但却收效甚微。笔者在本文中首先列举了写作老师批改作文的普遍特点,并指出这种做法将学生当做语言学习者而不是作者,老师是惟一的读者和权威,这样给出的评语对学生的写作并无多大的帮助。继而笔者提出了四条可行性建议和做法:1注重内容;2评语具体;3标准灵活;4同伴阅读。在结论部分笔者重申老师应该改变对学生作文作出反应的方式和方法以便学生更好地修改文章,最终使学生提高写作兴趣和写作水平。 | Hu Meizhu Anqing Teachers’ College | 2002 | Chinese Journal of Applied Linguistics2002，25，1： | 3 |
| 8 | Characteristics of Oil and Gas Accumulation in Yong'an-Meitai Area of the Fushan Depression,Beibuwan Basin,South China Sea显示文摘The Yong'an-Meitai area is the focus of the present exploration in the Fushan Depression,Beibuwan Basin,South China Sea. All oils from this area are geochemically characterized by higher Pr/Ph ratio,higher proportion of heavy molecular weight hydrocarbons,and higher proportion of C29 regular steranes,which indicate that the organic matter of source rocks might have been deposited in an oxidizing palaeoenvironment and be dominated by higher plant organic matter input. The oil from E3w2 (the second member of Weizhou Fm. of the Oligocene) has a much higher density,relatively higher Pr/nC17 and Ph/nC18 ratios,and a 'UCM-unresolved complex mixture' on gas chromatograms,which indicate that it has been slightly biodegraded. CPI and other terpane and sterane isomer ratios suggest they are all mature oils. The timing of oil charging in E3w2 and E2l1 (the first member of the Liushagang Fm. of the Eocene) determined by the homogenization temperatures of fluid inclusions and thermal evolution history are from 9-3 Ma and 8-3 Ma,respectively. Thus,the interpretation of E3w2 as a secondary reservoir is unlikely. The timing of oil charging is later than that of hydrocarbon generating and expulsion of Liushagang Fm. source rocks and trap formation,which is favorable for oil accumulation in this area. All molecular parameters that are used for tracing oil filling direction decrease with shallower burial depth,which suggests vertical oil migration. The widely occurring faults that penetrate through the source rocks of the Liushagang Fm. may serve as a fine oil charging conduit. | Li Meijun Wang Tieguan Liu Ju Zhang Meizhu Lu Hong Ma Qinglin Gao Lihui | 2007 | Petroleum Science2007，4，4： | 2 |
| 9 | Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9显示文摘 | Yuxuan Wu Dan Liang Yinghua Wang Meizhu Bai Wei Tang Shiming Bao Zhiqiang Yan Dangsheng Li Jinsong Li | 2013 | Cell Stem Cell2013，，6： | 2 |
| 10 | Alkyl Naphthalenes and Phenanthrenes:Molecular Markers for Tracing Filling Pathways of Light Oil and Condensate Reservoirs显示文摘冷凝物和轻油被高成熟, sterane 的低集中和 terpane biomarkers 和非烃部分的低内容通常描绘。作为结果,某使用得通常的 sterane, terpane 和 carbazole,在常规油水库的迁居参数可以在煤气的层析团 spectrometry (GCMS ) 为他们的差的信号噪音比率在冷凝物和轻油水库有某个限制。然而,萘,菲和他们的 methylated 取代者在在冷凝物和轻油的重要集中是在场的。拿 Fushan 消沉(在 Beibuwan 盆,北华南海) 作为一个例子,这份报纸第一次试图使用多不的芳香的烃(哼) 跟踪迁居方向的相关参数和为冷凝物和光的充满的小径给水库上油。结果显示出那 TMNr (即 1,3,7-TMN/(1,3,7-TMN + 1,2,5-TMN ) , TMN:trimethylnaphthalene )) , MPI-1 (即 1.5 自从热历史在古生代不能在更低的古生代的继任由于有效热指示物的缺乏客观地被重建。磷灰石和锆石(U-Th )/He thermochronometry 能被用作技术的一种新类型学习热历史并且构造沉积的盆高举。把年龄,磷灰石分裂磁道数据和等价 vitrinite 反射(% EVRo ) 基于测量磷灰石和锆石(U-Th )/He,在 Bachu 的 5 口井的 tectonothermal 历史高举被建模。当模特儿的结果证明有相对高的坡度一吗? | LI Meijun WANG Tieguan LIU Ju ZHANG Meizhu LU Hong MA Qinglin GAO Lihui | 2010 | Acta Geologica Sinica(English Edition)2010，84，5： | 2 |
| 11 | Neurobehavioral toxicity study of dibutyl phthalate on rats following in utero and lactational exposure显示文摘 | Li Yuanfeng Zhuang Meizhu Li Tao | 2009 | Journal of Applied Toxicology2009，29，： | 1 |
| 12 | Polyvalent mRNA vaccination elicited potent immune response to monkeypox virus surface antigens显示文摘Dear Editor,The global monkeypox(Mpox)outbreak leads to a surge in demand for vaccine.The Mpox vaccine in short supply is JYNNEOS(modified vaccinia ankara,MVA)due to less side effects,while ACAM2000 supply is abundant because of the strategic stockpile in U.s.1 The ACAM2000 replaced the first-generation smallpox vaccine Dryvax that has a questionable safety profile.However,the ACAM2000 is associated with high rate of myocarditis and pericarditis,and can cause severe illness in immunocompromised patients,foreshadowing the need to update the strategic stockpile. | Zhenhao Fang Valter S.Monteiro Paul A.Renauer Xingbo Shang Kazushi Suzuki Xinyu Ling Meizhu Bai Yan Xiang Andre Levchenko Carmen J.Booth Carolina Lucas Sidi Chen | 2023 | Cell Research2023，33，5： | 1 |
| 13 | Antidepressant-like effect of flavonoids extracted fromApocynum venetum leaves on brain monoamine levels and dopaminergic system显示文摘 | ZHENG Meizhu FAN Yajun SHI Dongfang | 2013 | Journal of Ethnopharmacology2013，147，1： | 1 |
| 14 | Melting intercalation method to prepare lauric acid/organophilic montmorillonite shape-stabilized phase change material显示文摘 | Meizhu Chen Shaoping Zheng Shaopeng Wu Guangji Xu | 2010 | Journal of Wuhan University of Technology - Mater Sci Ed2010，，4： | 1 |
| 15 | Anionic polymerization characteristics of the 1:1 complex of n-butylsodium and n,s-dibutylmagnesium(sodium tributylmagnesiate)显示文摘 | Liu Meizhu Kamienski C Morton M | 1986 | J Macromol Sci Chem1986，23，12： | 1 |
| 16 | A pH sensor based on the Ti02 nanotube array modified Tielectrode显示文摘 | Rongrong Zhao Meizhu Xu Jian Wang Guonan Chert | 2010 | Electrochimica Acta2010，55，20： | 1 |
| 17 | Pro- tective effects of flavonoid extract from Apocynum venetum leaves against corticosterone-induced neurotoxicity in PC12 cells显示文摘 | Zhang Meizhu Liu Chunming Pan Fengguang | 2011 | Cell Mol Neurobiol2011，31，3： | 1 |
| 18 | Utilization of silicone maintenance materials to improve the moisture sensitivity of asphalt mixtures 显示文摘 | Lin Juntao Chen Meizhu Wu Shaopeng | 2012 | Construction and Building Materials2012，，33： | 1 |
| 19 | Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9显示文摘Tissue-specific knockout technology enables the analysis of the gene function in specific tissues in adult mammals.However,conventional strategy for producing tissue-specific knockout mice is a time- and labor-consuming process,restricting rapid study of the gene function in vivo.CRISPR-Cas9 system from bacteria is a simple and efficient gene-editing technique,which has enabled rapid generation of gene knockout lines in mouse by direct injection of CRISPR-Cas9 into zygotes.Here,we demonstrate CRISPR-Cas9-mediated spermatogenic cell-specific disruption of Scp3 gene in testes in one step.We first generated transgenic mice by pronuclear injection of a plasmid containing Hspa2 promoter driving Cas9 expression and showed Cas9 specific expression in spermatogenic cells.We then produced transgenic mice carrying Hspa2 promoter driven Cas9 and constitutive expressed sgRNA targeting Scp3 gene.Male founders were infertile due to developmental arrest of spermatogenic cells while female founders could produce progeny normally.Consistently,male progeny from female founders were infertile and females could transmit the transgenes to the next generation.Our study establishes a CRISPR-Cas9-based one-step strategy to analyze the gene function in adult tissues by a temporal-spatial pattern. | Meizhu Bai Dan Liang Yinghua Wang Qing Li Yuxuan Wu Jinsong Li | 2016 | Journal of Genetics and Genomics2016，43，5： | 1 |
| 20 | Physical,Chemical and Rheological Properties of Waste Edible Vegetable Oil Rejuvenated Asphalt Binders显示文摘 | Chen Meizhu Leng Binbin Wu Shaopeng | 2014 | Construction and Building Materials2014，66，： | 1 |
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