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| 1 | Renewable Energy Transmission by HVDC Across the Continent:System Challenges and Opportunities显示文摘Rapid development of renewable energy in China is driving a major shift in the characteristics and control requirements of the electricity grid.Since the best renewable energy resources are far away from load centers in the east and southeast,transmission over long distances is required.Over 20 high-voltage DC(HVDC)transmission lines,with a combined capacity exceeding 150 GW,are in operation or are currently under construction.This rapid expansion of new generation and transmission capacities based on power electronics starts to change the characteristics of the grid,especially in areas where they concentrate,creating new stability problems and operational challenges.New system theories and technologies are required to support the development and operation of a future grid that relies more and more on power electronics.This paper highlights the characteristics of power electronics as used in renewable energy generation and HVDC transmission systems,discusses the impacts of these power-electronics-based assets on grid stability and operational requirements,and identifies opportunities for the development of both new system theories and system technologies to support a national energy policy that emphasizes the use of clean energy. | Jian Sun Mingjie Li Zhigang Zhang Tao Xu Jingbo He Haijiao Wang Guanghui Li | 2017 | CSEE Journal of Power and Energy Systems2017，3，4： | 43 |
| 2 | Multi-layer Quasi Three-dimensional Equivalent Model of Axial-Flux Permanent Magnet Synchronous Machine显示文摘Axial-flux permanent magnet synchronous machine(AFPMSM)enjoys the merits of high torque density and high efficiency,which make it one good candidate in the direct-drive application.The AFPMSM is usually analyzed based on the three-dimensional finite element method(3D FEM)due to its three-dimensional magnetic field distribution.However,the 3D FEM suffers large amount of calculation,time-consuming and is not suitable for the optimization of AFPMSM.Addressing this issue,a multi-layer quasi three-dimensional equivalent model of the AFPMSM is investigated in this paper,which could take the end leakage into consideration.Firstly,the multi-layer quasi three-dimensional equivalent model of the AFPMSM with single stator and single rotor is derived in details,including the equivalent processes and conversions of structure dimensions,motion conditions and electromagnetic parameters.Then,to consider the influence of end leakage on the performance,a correction factor is introduced in the multi-layer quasi three-dimensional equivalent model.Finally,the proposed multi-layer quasi three-dimensional equivalent model is verified by the 3D FEM based on an AFPMSM under different structure parameters.It demonstrates that the errors of flux linkage and average torque obtained by the multi-layer quasi three-dimensional equivalent model and 3D FEM are only around 2%although the structure parameters of the AFPMSM are varied.Besides,the computation time of one case based on the multi-layer quasi three-dimensional equivalent model is only 6 min,which is much less than that of the 3D FEM,1.8 h,under the same conditions.Thus,the proposed multi-layer quasi three-dimensional equivalent model could be used to optimize the AFPMSM and much time could be saved by this method compared with the 3D FEM. | Mingjie He Weiye Li Jun Peng Jiangtao Yang | 2021 | CES Transactions on Electrical Machines and Systems2021，5，1： | 7 |
| 3 | Land use change characteristics affected by water saving practices in Manas River Basin,China using Landsat satellite images显示文摘The characteristics and influencing factors of land use change under arid conditions were studied in the Manas River Basin in Xinjiang Region,Northwest China.Landsat satellite images acquired in 1976,1990,2000,2010 and 2015 over the study area were used as basic data.Land use change,the rate of change of land use,land use transfer and other aspects revealed the characteristics of land use change and related factors as influenced by water conditions in the basin.The results showed that:(1)Over nearly 50 years,land reclamation in the Manas River Basin resulted in the rapid expansion of an artificial oasis area,and promoted the process of‘oasis urbanization’,and accelerated the development of the river basin economy.(2)In 2000,the popularization of drip irrigation under mulch technology led to the rapid growth of cultivated land and development land in the watershed.Meanwhile,the water table declined in the desert area of the lower reaches of the river basin,and the area occupied by sparse shrub forest and grassland decreased.(3)Before popularization of water-saving technology,woodland,grassland and development land transformed to cultivated land in the amounts of 93.46 km^(2),2542.93 km^(2) and 137.53 km^(2),respectively,and woodland transformed in the amount of 189.64 km^(2).After water-saving technology was popularized,woodland,grassland and development land were transformed into cultivated land in the amounts of 567.41 km^(2),1756.2 km^(2) and 37.36 km^(2),respectively.(4)The popularization of water-saving technology made the dynamic degree of cultivated land and development land more active,and further increased landscape fragmentation and landscape heterogeneity.The level of urbanization development,the level of economic development and the dry humidity of the basin became the main factors affecting the change of land use in the basin. | Yang Guang Chen Dong He Xinlin Long Aihua Yang Mingjie Li Xiaolong | 2017 | International Journal of Agricultural and Biological Engineering2017，10，6： | 5 |
| 4 | Nickel single atom overcoordinated active sites to accelerate the electrochemical reaction kinetics for Li-S cathode显示文摘Lithium-sulfur(Li-S)batteries with high theoretical energy density are promising advanced energy storage devices.However,shuttling of dissolute lithium polysulfide(LiPSs)and sluggish conversion kinetics impede their applications.Herein,single nickel(Ni)atoms on two-dimensional(2D)nitrogen(N)-doped carbon with Ni-N_(4)-O overcoordinated structure(SANi-N_(4)-O/NC)are prepared and firstly used as a sulfur host of Li-S batteries.Due to the efficient polysulfides traps and highly LiPSs conversion effect of SANi-N_(4)-O/NC,the electrochemical performance of Li-S batteries obviously improved.The batteries can well operate even under high sulfur loading(5.8 mg cm^(-2))and lean electrolyte(6.1μL mg^(-1))condition.Meanwhile,density functional theory(DFT)calculations demonstrate that Ni single atom’s active sites decrease the energy barriers of conversion reactions from Li_(2)S_(8)to Li2S due to the strong interaction between SANi-N_(4)-O/NC and LiPSs.Thus,the kinetic conversion of LiPSs was accelerated and the shuttle effect is suppressed on SANi-N_(4)-O/NC host.This study provides a new design strategy for a 2D structure with single-atom overcoordinated active sites to facilitate the fast kinetic conversion of LiPSs for Li-S cathode. | Juan Zhu Xinyue Wang Tian Ke Mingji Jia Biyu Jin Yuanyuan Li Qiwei Yang Lanhui Ren Yongyuan Ren Dangguo Cheng Jianguo Lu Xiang Gao Qinggang He Yang Hou Xiaoli Zhan Qinghua Zhang | 2023 | Journal of Energy Chemistry2023，，3： | 2 |
| 5 | Seasonal variations, temperature dependence, and sources of size-resolved PM components in Nanjing, east China显示文摘Size-segregated ambient particulate matter(PM) samples were collected seasonally in suburban Nanjing of east China from 2016 to 2017 and chemically speciated. In both fine(< 2.1μm, PM2.1) and coarse(> 2.1 μm, PM>2.1) PM, organic carbon(OC) accounted for the highest fractions(26.9% ± 10.9% and 23.1% ± 9.35%) of all measured species, and NO3-lead in average concentrations of water-soluble inorganic ions(WSIIs). The size distributions of measured components were parameterized using geometric mean diameter(GMD). GMD values of NO_(3)^(-), Cl^(-), OC, and PM for the whole size range varied from < 2.1 μm in winter to > 2.1μm in warm seasons, which was due to the fact that the size distributions of semi-volatile components(e.g., NH4NO3, NH4Cl, and OC) had a dependency on the ambient temperature.Unlike OC, elemental carbon(EC), and elements, NH_(4)^(+), NO_(3)^(-), and SO_(4)^(2-)exhibited an increase trend in GMD values with relative humidity, indicating that the hygroscopic growth might also play a role in driving seasonal changes of PM size distributions. Positive matrix factorization was performed using compositional data of fine and coarse particles, respectively. The secondary formation of inorganic salts contributing to the majority(> 70%) of fine PM and 20.2% ± 19.9% of speciated coarse PM. The remaining coarse PM content was attributed to a variety of dust sources. Considering that coarse and fine PM had comparable mass concentrations, more attention should be paid to local dust emissions in future air quality plans. | Mingjie Xie Wei Feng Shuyan He Qin’geng Wang | 2022 | Journal of Environmental Sciences2022，34，11： | 2 |
| 6 | Analysis of aseismic performances of SRC frame supported short-leg pier shear wall structure with energy dissipation haunch brace 显示文摘 | Wu Yi He Mingji Zhou Yun | 2009 | Journal of Harbin Institute of Technology2009，41，2： | 1 |
| 7 | Molecular structure on the detoxification of fluorinated liquid crystal monomers with reactive oxidation species in the photocatalytic process显示文摘Fluorinated liquid crystal monomers(LCMs)are begun to emerge as new persistent organic pollutants.Herein,the structure-reactivity relationships of fluorinated LCMs 1,2,3-trifluoro-5-[3-(3-propylcyclohexyl)cyclohexyl]benzene(TPrCB),1,2-difluoro-4-[trans-4-(trans-4-propylcyclohexyl)cyclohexyl]benzene(DPrCB),4-[(trans,trans)-4'-(3-Buten-1-yl)[1,10-bicyclohexyl]-4-yl]-1,2-difluoro-benzene(BBDB)and 1-[4-(4-ethylcyclohexyl)cyclohexyl]-4(trifluoromethoxy)benzene(ECTB)subject to photocatalysis-generated oxidation species were investigated.The degradation rate constant of BBDB was 3.0,2.6,and 6.8 times higher than DPrCB,TPrCB and ECTB,respectively.The results reveal that BBDB,DPrCB and TPrCB had mainly negative electrostatic potential(ESP)regions which were vulnerable to electrophilic attack by h^(+),·OH and·O_(2)^(-),while ECTB was composed of mainly positive ESP regions which were vulnerable to nucleophilic attack by·OH and·O_(2)^(-).The detoxification processes of BBDB,DPrCB and TPrCB included carbon bond cleavage and benzene ring opening.However,the methoxy group of ECTB reduced the nucleophilic reactivity on the benzene ring,leading to slower detoxification efficiency.These findings may help to develop LCMs treatment technologies based on structure-reactivity relationships。 | Shaoxiong He Mingjie Shen Enya Wu Renli Yin Mingshan Zhu Lixi Zeng | 2022 | Environmental Science and Ecotechnology2022，，1： | 1 |
| 8 | Association of Interleukin 6 gene polymorphisms with genetic susceptibilities to spastic tetraplegia in males: A case-control study显示文摘 | Mingjie Chen Tongchuan Li Sheyu Lin Dan Bi Dengna Zhu Qing Shang Caiyun Ma Honglian Wang Lei Wang Yiting Zhang Lin He Changlian Zhu Qinghe Xing | 2013 | Cytokine2013，，： | 1 |
| 9 | Three dimensional digital measurement of the facet joint in normal and lumbar intervertebral disc herniation aged 13-18 years显示文摘To find out the normal lumbar facet joints between 13 and 18 years old,in order to provide anatomical basis for early diagnosis and therapy of lumbar disc herniation.CT imagings of 32 subjects with lumbar disc herniation aged from 13 to 18 years old in Inner Mongolia have been collected as the ALDH group,and 62 healthy subjects in the equal period had been chosen as the normal group.Continuous scanning lumbar tomography pix were imported into Mimics 21.0 for evaluation and size in DICOM format. | Wang Yidan Wang Haiyan Li Xiaohe Li Zhijun Wu Chao Zhang Yunfeng He Yujie Gao Mingjie Guan Huanhuan Dai Lina | 2021 | 解剖学杂志2021，44，S01： | 1 |
| 10 | A novel two- step fermentation process for improved arachidonic acid production by Mortierella alpina显示文摘 | Mingjie Jin He Huang Ai-Hua Xiao | 2008 | Biotechnology Letters2008，30，6： | 1 |
| 11 | Influences of drought on the stability of an alpine meadow ecosystem显示文摘Drought plays a prominent role in affecting ecosystem stability and ecosystem productivity.Based on eddy covariance and climatic observations during 2012-2020,the Fisher discriminant analysis method was employed to accurately detect drought occurrences.Furthermore,the ecosystem water sensitivity and its resistance to drought were quantified to evaluate the ecosystem stability.The results showed that the alpine meadow suffered drought most frequently at the beginning of the growing seasons.However,drought during the peak growing seasons reduced the gross primary productivity(GPP)the most,by 30.5±15.2%.In the middle of the peak growing seasons,the ecosystem water sensitivity was weak,and thus,the resistance to drought was strong,which resulted in high ecosystem stability.At the beginning and end of the peak growing seasons,the ecosystem stability was relatively weak.Ecosystem stability was positively related to the corresponding multiyear average soil water content(SWC_(ave)).However,drought occurring during high SWC_(ave)periods led to larger reductions in GPP,which indicated that the inhibitory effects of drought on ecosystems were more dependent on the occurrence time of droughts than on ecosystem stability. | Tao Zhang Ximeng Ji Mingjie Xu Guang Zhao Zhoutao Zheng Yuanyuan Tang Ning Chen Juntao Zhu Yongtao He Yangjian Zhang | 2022 | Ecosystem Health and Sustainability2022，8，1： | 0 |
| 12 | Step‑by‑Step Modulation of Crystalline Features and Exciton Kinetics for 19.2%Efficiency Ortho‑Xylene Processed Organic Solar Cells显示文摘With plenty of popular and effective ternary organic solar cells(OSCs)construction strategies proposed and applied,its power conversion efficiencies(PCEs)have come to a new level of over 19%in single-junction devices.However,previous studies are heavily based in chloroform(CF)leaving behind substantial knowledge deficiencies in understanding the influence of solvent choice when introducing a third component.Herein,we present a case where a newly designed asymmetric small molecular acceptor using fluoro-methoxylated end-group modification strategy,named BTP-BO-3FO with enlarged bandgap,brings different morphological evolution and performance improvement effect on host system PM6:BTP-eC9,processed by CF and ortho-xylene(o-XY).With detailed analyses supported by a series of experiments,the best PCE of 19.24%for green solvent-processed OSCs is found to be a fruit of finely tuned crystalline ordering and general aggregation motif,which furthermore nourishes a favorable charge generation and recombination behavior.Likewise,over 19%PCE can be achieved by replacing spin-coating with blade coating for active layer deposition.This work focuses on understanding the commonly met yet frequently ignored issues when building ternary blends to demonstrate cutting-edge device performance,hence,will be instructive to other ternary OSC works in the future. | Bosen Zou Weiwei Wu Top Archie Dela Pena Ruijie Ma Yongmin Luo Yulong Hai Xiyun Xie Mingjie Li Zhenghui Luo Jiaying Wu Chuluo Yang Gang Li He Yan | 2024 | Nano-Micro Letters2024，16，2： | 0 |
| 13 | Synergy of in-situ heterogeneous interphases tailored lithium deposition显示文摘The implementation of a robust artificial solid electrolyte interphase(ASEI)to replace the unstable natural SEI can regulate lithium deposition behaviors and avoid the safety hazards caused by dendrites permeation in lithium metal batteries.Despite of devoted efforts in tailoring components of ASEI,the intrinsic mechanism of interfacial synergy within the heterogeneous interphases has not been well elucidated yet.Herein,we show that the lithium plating/striping behaviors can be substantially enhanced(over 900 h with an overpotential of less than 20 mV at 1 mA·cm^(−2)in Li|Li symmetric cells and 146 cycles in anode-free cells)by regulating the heterogeneous interphases.This favorable ASEI composed of LiF and Li_(3)N components can be in-situ generated during cycling by large-scale fabricated fluorinated boron nitride coatings.Further,the synergy of each heterogeneous component within ASEI was explored theoretically and experimentally.Li_(3)N has high adsorption energy and low ion diffusion barrier,which facilitates the transport of lithium ions and avoids its local accumulation to evolve into dendrites.Both the substrate and LiF are interfacially stable with high electron tunneling barriers,preventing the electrolyte decomposition and parasitic reactions.Finally,the high stiffness of the boron nitride also ensures lithium dendrites are suppressed once they grow,providing a stable environment for long-term cycling of lithium metal batteries. | Yinuo Li Anjun Hu Xingdong Gan Miao He Jun Zhu Wei Chen Yin Hu Tianyu Lei Fei Li Yaoyao Li Yuxin Fan Fan Wang Mingjie Zhou An Wen Baihai Li | 2023 | Nano Research2023，16，6： | 0 |
| 14 | Remodeling “cold” tumor immune microenvironment via epigenetic-based therapy using targeted liposomes with in situ formed albumin corona显示文摘There is a close connection between epigenetic regulation,cancer metabolism,and immunology.The combination of epigenetic therapy and immunotherapy provides a promising avenue for cancer management.As an epigenetic regulator of histone acetylation,panobinostat can induce histone acetylation and inhibit tumor cell proliferation,as well as regulate aerobic glycolysis and reprogram intratumoral immune cells.JQ1 is a BRD4 inhibitor that can suppress PD-L1 expression.Herein,we proposed a chemo-free,epigenetic-based combination therapy of panobinostat/JQ1 for metastatic colorectal cancer.A novel targeted binary-drug liposome was developed based on lactoferrin-mediated binding with the LRP-1 receptor.It was found that the tumor-targeted delivery was further enhanced by in situ formation of albumin corona.The lactoferrin modification and endogenous albumin adsorption contribute a dual-targeting effect on the receptors of both LRP-1 and SPARC that were overexpressed in tumor cells and immune cells(e.g.,tumor-associated macrophages).The targeted liposomal therapy was effective to suppress the crosstalk between tumor metabolism and immune evasion via glycolysis inhibition and immune normalization.Consequently,lactic acid production was reduced and angiogenesis inhibited;TAM switched to an anti-tumor phenotype,and the anti-tumor function of the effector CD8+T cells was reinforced.The strategy provides a potential method for remodeling the tumor immune microenvironment(TIME). | Yang He Yuefei Fang Meng Zhang Yuge Zhao Bin Tu Mingjie Shi Bahtiyor Muhitdinov Akmal Asrorov Qin Xu Yongzhuo Huang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 0 |
| 15 | SUMOylation-triggered ALIX activation modulates extracellular vesicles circTLCD4-RWDD3 to promote lymphatic metastasis of non-small cell lung cancer显示文摘Lymph node(LN)metastasis is one of the predominant metastatic routes of non-small cell lung cancer(NSCLC)and is considered as a leading cause for the unsatisfactory prognosis of patients.Although lymphangiogenesis is well-recognized as a crucial process in mediating LN metastasis,the regulatory mechanism involving lymphangiogenesis and LN metastasis in NSCLC remains unclear.In this study,we employed high-throughput sequencing to identify a novel circular RNA(circRNA),circTLCD4-RWDD3,which was significantly upregulated in extracellular vesicles(EVs)from LN metastatic NSCLC and was positively associated with deteriorated OS and DFS of patients with NSCLC from multicenter clinical cohort.Downregulating the expression of EV-packaged circTLCD4-RWDD3 inhibited lymphangiogenesis and LN metastasis of NSCLC both in vitro and in vivo.Mechanically,circTLCD4-RWDD3 physically interacted with hnRNPA2B1 and mediated the SUMO2 modification at K108 residue of hnRNPA2B1 by upregulating UBC9.Subsequently,circTLCD4-RWDD3-induced SUMOylated hnRNPA2B1 was recognized by the SUMO interaction motif(SIM)of ALIX and activated ALIX to recruit ESCRT-III,thereby facilitating the sorting of circTLCD4-RWDD3 into NSCLC cell-derived EVs.Moreover,EV-packaged circTLCD4-RWDD3 was internalized by lymphatic endothelial cells to activate the transcription of PROX1,resulting in the lymphangiogenesis and LN metastasis of NSCLC.Importantly,blocking EV-mediated transmission of circTLCD4-RWDD3 via mutating SIM in ALIX or K108 residue of hnRNPA2B1 inhibited the lymphangiogenesis and LN metastasis of NSCLC in vivo.Our findings reveal a precise mechanism underlying SUMOylated hnRNPA2B1-induced EV packaging of circTLCD4-RWDD3 in facilitating LN metastasis of NSCLC,suggesting that EV-packaged circTLCD4-RWDD3 could be a potential therapeutic target against LN metastatic NSCLC. | Xiayao Diao Chao Guo Hanhao Zheng Ke Zhao Yuming Luo Mingjie An Yan Lin Jiancheng Chen Yuanlong Li Yuting Li Xuehan Gao Jiaqi Zhang Mengxin Zhou Wenliang Bai Lei Liu Guige Wang Lanjun Zhang Xiaotian He Rusi Zhang Zhihua Li Changhao Chen Shanqing Li | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 16 | 3D Printed Antennas for 5G Communication:Current Progress and Future Challenges显示文摘With the advent of 5G and future trends for communication systems moving to millimeter wave(MMW)and higher frequencies,antennas will be required to have high gain,wide bandwidth,and low losses.3D printing realizes structures by sequential stacking layer-by-layer,which enables the manufacturing of antennas with ar-bitrary shapes in a cheaper,faster,and flexible manner.This study provides a review of current state-of-the-art 3D printed antennas for different frequencies.First,an overview of 3D printing technology is presented.A huge number of 3D printed antennas,categorized by their material composition,have been described,including poly-mer,metallic,ceramic,composite material,and multi-material integrated antennas.Finally,the main challenges and prospects are discussed to provide insight into how 3D printing can be further progressed in antenna manu-facturing. | Yaru Wang Xueqin Zhang Ruyue Su Mingji Chen Chujing Shen Hao Xu Rujie He | 2023 | Chinese Journal of Mechanical Engineering(Additive Manufacturing Frontiers)2023，2，1： | 0 |
| 17 | Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1显示文摘Aging increases the risks of various diseases and the vulnerability to death.Cellular senescence is a hallmark of aging that contributes greatly to aging and aging-related diseases.This study demonstrates that extracellular vesicles from human urine-derived stem cells(USC-EVs)efficiently inhibit cellular senescence in vitro and in vivo.The intravenous injection of USC-EVs improves cognitive function,increases physical fitness and bone quality,and alleviates aging-related structural changes in different organs of senescence-accelerated mice and natural aging mice.The anti-aging effects of USC-EVs are not obviously affected by the USC donors’ages,genders,or health status.Proteomic analysis reveals that USC-EVs are enriched with plasminogen activator urokinase(PLAU)and tissue inhibitor of metalloproteinases 1(TIMP1).These two proteins contribute importantly to the anti-senescent effects of USC-EVs associated with the inhibition of matrix metalloproteinases,cyclin-dependent kinase inhibitor 2A(P16INK4a),and cyclin-dependent kinase inhibitor 1A(P21cip1).These findings suggest a great potential of autologous USC-EVs as a promising anti-aging agent by transferring PLAU and TIMP1 proteins. | Shanshan Rao Zehui He Zun Wang Hao Yin Xiongke Hu Yijuan Tan Tengfei Wan Hao Zhu Yi Luo Xin Wang Hongming Li Zhenxing Wang Xinyue Hu Chungu Hong Yiyi Wang Mingjie Luo Wei Du Yuxuan Qian Siyuan Tang Hui Xie Chunyuan Chen | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 18 | Changes in the Features of Major Contradictions显示文摘Immediately after humanity entered the new century,the worldseems somewhat a bit turbulent because of the following events.First are the September 11 events,which were followed by U.S.militaryattacks against Afghanistan.Last year witnessed the continuity of Arab-Israel conflicts in the Middle East,and at the very beginning of the newyear,the United States dispatched warships to the Persian Gulf for a“war to topple the Saddam regime”.The Democratic People’s Republicof Korea(DPRK)suddenly declared that it has decided to withdraw fromthe Nuclear Non-proliferation Treaty just after Iraq stated that it wasdetermined to resist the United States.Whither the world in the new year?This is a question that all of the academic circles on international relationsare trying to answer.To this end,on January 9,2002,we speciallyinvited Lu Zhongwei,Research Professor and President of China Instituteof Contemporary International Relations and some other experts oninternational relations from the institute to hold a symposium with the theme“Prospect on World Trend 2003.”Now we present their discussions as anew-year gift to our readers who are always in support of our journal.Wehope this would do a bit for their mastery of international situation. | Lu Zhongwei Wang Zaibang Ji Zhiye Fu Mengzi Feng Zhongping Yang Bojiang Dao Shulin Yang Mingjie Lin Limin Su Jingxiang He Xiquan | 2003 | Contemporary International Relations2003，13，2： | 0 |
| 19 | Damage Analysis of Circulating Soaked Rock Sample Based on Sonic-test Trials显示文摘 | HE Ye ZHAO Mingjie CAI Ying | 2014 | International English Education Research2014，，12： | 0 |
| 20 | Analysis of Bacterial Communities Present in Agaricus bisporus Phase Ⅱ Compost Using Amplified Ribosomal DNA Restriction Analysis(ARDRA)(Ⅱ)——Analysis of Bacterial Communities显示文摘Changes in the bacterial communities inhabiting Agaricus bisporus compost during Phase Ⅱ treatment were monitored using amplified rDNA restriction analysis(ARDRA).Sequence analysis data indicated that the bacterial communities associated with the compost samples were far richer in composition when determined by ARDRA compared with traditional methods based on bacterial isolation.Furthermore,the composition of the communities,especially in terms of the dominant bacteria during each of the four treatment stages(beginning of Phase Ⅱ,after pasteurization,5 days aeration,end of Phase Ⅱ,respectively),were very different.Restriction endonuclease digestion of mainly bacterial clones from four 16S rDNA libraries,C1,C3,C6 and C7,previously constructed on the basis of 16S rDNA fragments amplified from the four different stages of the Phase Ⅱ composting process,revealed the presence of unique microbial types.Thermophilic bacteria belonging to the Bacilli,and previously unreported strains of Trichococcus,Planococcus,Caryophanon,and subclass γ-Proteobacteria,were identified among the clones from the compost sampled during the aeration period(C6).Bacteria belonging to Thermus thermophilus and subclass α-Proteobacteria were detected in C1 and C7 composts sampled at the beginning and the end of Phase Ⅱ,respectively.Clones of some uncultured bacteria were also scored. | HE Lihong YU Rongli CHEN Mingjie PAN Yingjie | 2009 | 食用菌学报2009，16，1： | 0 |
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