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| 1 | Anti-loosening performance of coatings on fasteners subjected to dynamic shear load显示文摘This paper investigates the self-loosening of threaded fasteners subjected to dynamic shear load. Three kinds of typical coatings, PTFE, MoS_2, and TiN applied to bolts and nuts, are tested in this investigation. The study experimentally examines the loosening mechanisms of fasteners and assesses the anti-loosening performance of the three tested coatings based on their tightening characteristics, loosening curves, and the damage of thread surface. Additionally, the anti-loosening performance of the three coatings is compared under different load forms. The results indicate that the PTFE and MoS_2 coatings have significant anti-loosening effect, whereas the anti-loosening performance of Ti N coating is not satisfactory. It is also found that an appropriate increase of the initial tightening torque can significantly improve the anti-loosening effect. In addition, the microscopic analyses of PTFE and MoS2 coating reveal that a reduced initial tightening torque leads to fretting wear on the thread contact surfaces of fasteners, thereby aggravating the damage. | Junbo ZHOU Jianhua LIU Huajiang OUYANG Zhenbing CAI Jinfang PENG Minhao ZHU | 2018 | Friction2018，6，1： | 10 |
| 2 | Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies显示文摘Neutralizing monoclonal antibodies(nAbs)to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)represent promising candidates for clinical intervention against coronavirus disease 2019(COVID-19).We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain(RBD)of the viral spike(S)protein and the receptor angiotensin converting enzyme 2(ACE2).However,the structural basis for their potent neutralizing activity remains unclear.Here,we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2.The bivalent binding of the full-length IgG is found to associate with more RBDs in the“up”conformation than the monovalent binding of Fab,perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein.Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities.This work provides a structural basis for further understanding the mechanism of nAbs,especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit. | Renhong Yan Ruoke Wang Bin Ju Jinfang Yu Yuanyuan Zhang Nan Liu Jia Wang Qi Zhang Peng Chen Bing Zhou Yaning Li Yaping Shen Shuyuan Zhang Long Tian Yingying Guo Lu Xia Xinyue Zhong Lin Cheng Xiangyang Ge Juanjuan Zhao Hong-Wei Wang Xinquan Wang Zheng Zhang Linqi Zhang Qiang Zhou | 2021 | Cell Research2021，31，5： | 4 |
| 3 | MIT1, encoding a 15-cis-ζ-carotene isomerase, regulates tiller number and stature in rice显示文摘Lateral branching is an important determinant of shoot architecture and crop yield. The plant hormone strigolactone (SL) inhibits lateral bud outgrowth in various plant species. Deficiencies in SL biosynthesis and signal transduction result in excessive outgrowth of lateral buds (Stirnberg et al., 2002;Sorefan et al., 2003). | Lihua Liu Mengmeng Ren Peng Peng Yan Chun Lu Li Jinfeng Zhao Jingjing Fang Lixiang Peng Jijun Yan Jinfang Chu Yiqin Wang Shoujiang Yuan Xueyong Li | 2021 | Journal of Genetics and Genomics2021，48，1： | 3 |
| 4 | Fretting Wear Behavior of Medium Carbon Steel Modified by Low Temperature Gas Multi-component Thermo-chemical Treatment显示文摘The introduction of surface engineering is expected to be an effective strategy against fretting damage. A large number of studies show that the low gas multi-component (such as carbon, nitrogen, sulphur and oxygen, etc) thermo-chemical treatment(LTGMTT) can overcome the brittleness of nitriding process, and upgrade the surface hardness and improve the wear resistance and fatigue properties of the work-pieces significantly. However, there are few reports on the anti-fretting properties of the LTGMTT modified layer up to now, which limits the applications of fretting. So this paper discusses the fretting wear behavior of modified layer on the surface of LZ50 (0.48%C) steel prepared by low temperature gas multi-component thermo-chemical treatment (LTGMTT) technology. The fretting wear tests of the modified layer flat specimens and its substrate (LZ50 steel) against 52100 steel balls with diameter of 40 mm are carried out under normal load of 150 N and displacement amplitudes varied from 2 μm to 40 μm. Characterization of the modified layer and dynamic analyses in combination with microscopic examinations were performed through the means of scanning electron microscope(SEM), optical microscope(OM), X-ray diffraction(XRD) and surface profilometer. The experimental results showed that the modified layer with a total thickness of 60 μm was consisted of three parts, i.e., loose layer, compound layer and diffusion layer. Compared with the substrate, the range of the mixed fretting regime(MFR) of the LTGMTT modified layer diminished, and the slip regime(SR) of the modified layer shifted to the direction of smaller displacement amplitude. The coefficient of friction(COF) of the modified layer was lower than that of the substrate in the initial stage. For the modified layer, the damage in partial slip regime(PSR) was very slight. The fretting wear mechanism of the modified layer both in MFR and SR was abrasive wear and delamination. The modified layer presented better wear resistance than the substrate in PSR and MFR; however, in SR, the wear resistance of the modified layer decreased with the increase of the displacement amplitudes. The experimental results can provide some experimental bases for promoting industrial application of LTGMTT modified layer in anti-fretting wear. | LUO Jun ZHENG Jianfeng PENG Jinfang HE Liping ZHU Minhao | 2010 | Chinese Journal of Mechanical Engineering2010，23，3： | 3 |
| 5 | Expression levels of CD28, CTLA?4, PD?1 and Tim?3 as novel indicatorsof T?cell immune function in patients with chronic hepatitis B virus infection显示文摘 | Lin Wang Chunnan Zhao Qunxin Peng Jinfang Shi Guohao Gu | 2014 | Biomedical Reports2014，，2： | 2 |
| 6 | Development of a novel cycling impact–sliding wear rig to investigate the complex friction motion显示文摘In many industrial devices, impact-sliding wear is caused by a variety of complex vibrations between the contacted interfaces. Under actual conditions, impact and sliding motions do not occur in only one direction, and different complex impact-sliding motions exist on the tribology surfaces. In this study, an impact-sliding wear test rig is developed to investigate the wear effect of different complex motions. Using this rig, multi-type impact-sliding wear effects are realized and measured, such as those derived from unidirectional, reciprocating, and multi-mode combination motions. These three types of impact–sliding wear running behavior are tested and the wear damage mechanism is discussed. | Zhenbing CAI Zhiqiang CHEN Yang SUN Jianying JIN Jinfang PENG Minhao ZHU | 2019 | Friction2019，7，1： | 2 |
| 7 | Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle显示文摘Whether glucose is predomi nantly metabolized via oxidative phosphorylati on or glycolysis differs between quiesce nt versus proliferating cells,iincluding tumor cells.However,how glucose metabolism is coordinated with cell cycle in mammalian cells remains elusive.Here,we report that mammalian cells predominantly utilize the tricarboxylic acid(TCA)cycle in G1 phase,but prefer glycolysis in S phase.Mechanistically,coupling cell cycle with metabolism is largely achieved by timely destruction of IDH1/2,key TCA cycle enzymes,in a Skp2-dependent manner.As such,depleting SKP2 abolishes cell cycle-dependent fluctuation of IDH1 protein abundance,leading to reduced glycolysis in S phase.Furthermore,elevated Skp2 abundance in prostate cancer cells destabilizes IDH1 to favor glycolysis and subsequent tumorigenesis.Therefore,our study reveals a mechanistic link between two cancer hallmarks,aberrant cell cycle and addiction to glycolysis,and provides the underlying mechanism for the coupling of metabolic fluctuation with periodic cell cycle in mammalian cells. | Jing Liu Yunhua Peng Le Shi Lixin Wan Hiroyuki Inuzuka Jiangang Long Jianping Guo Jinfang Zhang Min Yuan Shuangxi Zhang Xun Wang Jing Gao Xiangpeng Dai Shozo Furumoto Lijun Jia Pier Paolo Pandolfic John M.Asara William G.Kaelin Jr. Jjankang Liu Wenyi Wei | 2021 | Cell Research2021，31，1： | 2 |
| 8 | Creation of fragrant sorghum by CRISPR/Cas9显示文摘Sorghum,the fifth largest cereal crop,has high value as a staple food and raw material for liquor and vinegar brewing.Due to its high biomass and quality,it is also used as the second most planted silage resource.No fragrant sorghums are currently on the market.Through CRISPR/Cas9-mediated knockout of Sb BADH2,we obtained sorghum lines with extraordinary aromatic smell in both seeds and leaves.Animal feeding experiments showed that fragrant sorghum leaves were attractable.We believe this advantage will produce great value in the sorghum market for both grain and whole biomass forage. | Dan Zhang Sanyuan Tang Peng Xie Dekai Yang Yaorong Wu Shujing Cheng Kai Du Peiyong Xin Jinfang Chu Feifei Yu Qi Xie | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 9 | Tenglong Buzhong granules(藤龙补中颗粒) inhibits the growth of SW620 human colon cancer in vivo显示文摘OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD), i.e Tenglong Buzhong granules(藤龙补中颗粒, TBG), in human SW620 colon cancer. METHODS: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG(2.56 g/kg, once per day) and/or 5-Fu(104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry. RESULTS: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anticancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3,-8, and-9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a(CDKN1a) and cyclin-dependent kinase inhibitor 2a(CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein(RB) and expression of cyclin E1(CCNE1) and cyclindependent kinases(CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor(VEGF) and hypoxiainducible factor-1α(HIF-1α). CONCLUSIONS: TBG inhibits SW620 colon cancer growth, induces apoptosis via SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu. | LI Miao ZHENG Jialu WANG Shuangshuang CHEN Lei PENG Xiao CHEN Jinfang AN Hongmei HU Bing | 2022 | Journal of Traditional Chinese Medicine2022，42，5： | 1 |
| 10 | Problems in the Biogas Construction in Weihui City and Development Strategies显示文摘Taking the demonstration biogas construction city in Henan Province-Weihui City as the survey and research city, we give an overview of the industrial and agricultural base and current situation of biogas development in Weihui City, and analyze the main problems in biogas construction, such as simple development mode of biogas adopted by rural households, laggard construction of large and medium-sized biogas projects, unsound service system for biogas development and failure to comprehensively use biogas resources. According to the practical work experience, we sum up the following. | Yilun WANG Chao YUAN Qian ZHANG Jinfang TAN Peng ZHAO | 2013 | Asian Agricultural Research2013，5，2： | 0 |
| 11 | Optimization of several surface treatment processes for alleviating fretting damage of a locking pin显示文摘The operational safety and reliability of a variable gauge train are affected by the anti-fretting wear performance of the locking mechanism.The main purpose of this study is to optimize the surface treatment process for a locking pin material under actual service conditions to alleviate fretting damage.Based on the two basic principles of surface strengthening and friction reduction,a substrate(AISI 4135 steel)surface was treated by laser quenching(LQ),plasma nitriding(PN),and bonded MoS2 coating.Systematic fretting wear tests were conducted,and the wear behavior and damage mechanism of various treated surfaces were comprehensively investigated.The results indicate that the wear resistances of the LQ-and PN-treated surfaces were significantly improved,and their main wear mechanisms were abrasive wear,delamination,and oxidation wear.The MoS_(2) coating exhibits the lowest friction coefficient and energy dissipation due to its self-lubricating property,but it incurs the highest wear rate and failure in the form of plastic deformation.Furthermore,the rough compound layer with a high hardness on the PN-treated surface is conducive to the formation and maintenance of the third-body contact at the fretting interface,consequently resulting in a significant reduction in wear.An optimal surface treatment process for alleviating fretting damage of the locking pin is recommended via comprehensive evaluation,which provides a reference for the anti-fretting protection of related mechanical components. | Jifan HE Zhenbing CAI Yanping REN Jinfang PENG Jianhua LIU Minhao ZHU | 2022 | Friction2022，10，8： | 0 |
| 12 | Author Correction:Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle显示文摘The author affiliations were mixed up in the previous published version.In addition,the author contribution section missed a statement of The idea was conceived by J.K.L.,W.W.and This correction does not affect the description of the results or the conclusion of this work.We apologize for any inconvenience this may have caused. | Jing Liuc Yunhua Peng Le Shi Lixin Wanc Hiroyuki Inuzuka Jiangang Long Jianping Guo Jinfang Zhang Min Yuan Shuangxi Zhang Xun Wangc Jing Gao Xiangpeng Dai Shozo Furumoto Lijun Jia Pier Paolo Pandolfi John M.Asara William G.Kaelin Jr. Jiankang Liu Wenyi Wei | 2021 | Cell Research2021，31，1： | 0 |
| 13 | Burden of Skin Disease—China,1990−2019显示文摘Summary What is already known about this topic?Skin diseases are common,affect society and individuals,and have high incidences to relapse,which reduces the quality of life.In 2019,skin diseases were the seventh leading global causes of years lived with disability(YLDs).What is added by this report?All-age disability-adjusted life years(DALYs)and YLDs from skin diseases have been steadily increasing in China from 1990 through 2019,although with a decline in the standardized rate of years of life lost(YLLs).In 2019,dermatitis was the leading cause of YLLs among people over the age of 15 years,while viral skin diseases had the greatest burden among people under 15 years.Acne vulgaris increased significantly among people aged 15–49 years,and psoriasis increased among people over 50 years.The male∶female ratio of DALYs lost due to skin disease did not change between 1990 and 2019. | Dan Peng Jinfang Sun Jinyi Wang Xiao Qi Guoxing Li | 2021 | China CDC weekly2021，3，22： | 0 |
| 14 | Friction and Wear Properties of High-velocity Oxygen Fuel Sprayed WC-17Co Coating under Rotational Fretting Conditions显示文摘Rotational fretting which exist in many engineering applications has incurred enormous economic loss. Thus, accessible methods are urgently needed to alleviate or eliminate damage by rotational fretting. Surface engineering is an effective approach that is successfully adopted to enhance the ability of components to resist the fretting damage. In this paper, using a high-velocity oxygen fuel sprayed(HVOF) technique WC-17 Co coating is deposited on an LZ50 steel surface to study its properties through Vickers hardness testing, scanning electric microscope(SEM), energy dispersive X-ray spectroscopy(EDX), and X-ray diffractrometry(XRD). Rotational fretting wear tests are conducted under normal load varied from 10 N to 50 N, and angular displacement amplitudes vary from 0.125° to 1°. Wear scars are examined using SEM, EDX, optical microscopy(OM), and surface topography. The experimental results reveal that the WC-17 Co coating adjusted the boundary between the partial slip regime(PSR) and the slip regime(SR) to the direction of smaller amplitude displacement. As a result, the coefficients of friction are consistently lower than the substrate's coefficients of friction both in the PSR and SR. The damage to the coating in the PSR is very slight. In the SR, the coating exhibits higher debris removal efficiency and load-carrying capacity. The bulge is not found for the coating due to the coating's higher hardness to restrain plastic flow. This research could provide experimental bases for promoting industrial application of WC-17 Co coating in prevention of rotational fretting wear. | LUO Jun CAI Zhenbing MO Jiliang PENG Jinfang ZHU Minhao | 2016 | Chinese Journal of Mechanical Engineering2016，29，3： | 0 |
| 15 | Vitamin B6 prevents Isocarbophos-induced posterior cerebral artery injury in offspring rats through up-regulating S1P receptor expression显示文摘We have previously reported that the long-term exposure of Isocarbophos,a kind of organophosphorus compounds,induces vascular dementia(VD)in rats.Studies have also shown that organophosphorus compounds have adverse effects on offsprings.Vitamin B6 is a coenzyme mainly involved in the regulation of metabolism and has been demonstrated to ameliorate VD.Sphingosine-1-phosphate(S1P),a biologically active lipid,plays a vital role in the cardiovascular system.However,whether S1P is involved in the therapeutic effects of Vitamin B6 on posterior cerebral artery injury has yet to be further answered.In the present study,we aimed to explore the potential influence of Vitamin B6 on Isocarbophos-induced posterior cerebral artery injury in offspring rats and the role of the S1P receptor in this process.We found that Vitamin B6 significantly improves the vasoconstriction function of the posterior cerebral artery in rats induced by Isocarbophos by the blood gas analysis and endothelium-dependent relaxation function assay.We further demonstrated that Vitamin B6 alleviates the Isocarbophos-induced elevation of ICAM-1,VCAM-1,IL-1,and IL-6 by using the enzyme-linked immunosorbent assay kits.By performing immunofluorescence and the western blot assay,we revealed that Vitamin B6 prevents the down-regulation of S1P in posterior cerebral artery injury.It is worth noting that Fingolimod,the S1P inhibitor,significantly inhibits the Vitamin B6-induced up-regulation of S1P in posterior cerebral artery injury.Collectively,our data indicate that Vitamin B6 may be a novel drug for the treatment of posterior cerebral artery injury and that S1P may be a drug target for its treatment. | Yanhua Liu Kunli Yang Ling Wang Jinfang Yang Yang Wang Hu Luo Peng Li Yaling Yin | 2021 | Acta Biochimica et Biophysica Sinica2021，53，12： | 0 |
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