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| 1 | Protective effects of polydatin against CCl_4-induced injury to primarily cultured rat hepatocytes显示文摘NTRODUCTIONPolygonumcuspidatumSieb.etZucc.(Polygonaceae)isatraditionalChineseherbaldrug,withbitertasteandcoldnature.Itmainly... | HUANG Zhao Sheng, WANG Zong Wei, LIU Ming Ping, ZHONG Shi Qing, LI Qiao Mei and RONG Xiang Lu | 1999 | World Journal of Gastroenterology1999，5，1： | 24 |
| 2 | The Aging Feature of Al-L-Cu-Mg-Zr Alloy Containing Sc显示文摘The aging behaviors of Al 1.42%Li 2.41%Cu 0.93%Mg 0.073%Zr 0.17%Sc(mass fraction, the same below) alloy at room temperature, 160 ℃ , and 160 ℃ after 8% pre deformation were studied respectively by hardness measurement. The microstructure of the alloy in various aging conditions was observed by TEM. The results show that the main precipitations of the alloy in quenching condition are the particles containing Sc and Zr which have certain coherent relation with the matrix. Addition of Sc in Al Li Cu Mg Zr alloy will be favorable to promoting precipitation. The particles can serve as preferred nucleation sites for δ′ phases which accelerate the aging hardening rate at initial aging. The main hardening phases of the alloy aged at 160 ℃ are δ′ and δ′/β′ composite precipitates. The size of the composite precipitates is very small (nanometer size). The composite precipitates will preclude efficiently the formation concentrative slip location and will improve the mechanical properties of the alloy. S′ phase will occur in the alloy aged at 160 ℃ after 8% pre deformation. It is found that 8% pre deformation has no obvious influence on the precipitation of the composite phase. | TAN Cheng yu,ZHENG Zi qiao,XIA Chang qing,LIANG Ying (Department of Materials Science and Engineering, Central South University of Technology, Changsha 410083, China) | 2000 | Journal of Central South University2000，12，2： | 22 |
| 3 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 4 | Principles and technology for stepwise utilization of resources for mitigating deep mine heat hazards显示文摘As is well known, deep mines are hot. As mining depth increases, the temperature of the surrounding rock also increases. This seriously affects mine safety and production and has restricted the exploitation of deep coal resources. Therefore, reducing the working face temperature to improve working conditions by controlling these heat hazards is an urgent problem. Considering problems in cooling deep mines both domestically and abroad along with the actual conditions of the Zhangshuanglou coal mine, we propose a HEMS technology that uses heat resources from deep mines in a stepwise manner. HEMS means a high temperature ex-change machinery system. Mine inrush-water is used as a source of cooling. Twice the energy is extracted from the mine inrush water. Heat is used for building heating in the winter and cold water is used for cooling buildings in the summer. This opens a new technology for stepwise utilization of heat energy in deep mines. Energy conservation and reduced pollution, an improved environment and sustainable economic development are realized by this technique. The economic and social effects are obvious and illustrate a good prospect for the application and extension of the method. | HE Manchao CAO Xiuling XIE Qiao YANG Jiahua QI Ping YANG Qing CHEN Xueqian | 2010 | Mining Science and Technology2010，20，1： | 15 |
| 5 | The prognostic and therapeutic implications of circulating tumor cell phenotype detection based on epithelial-mesenchymal transition markers in the first-line chemotherapy of HER2-negative metastatic breast cancer显示文摘Background:Epithelial-mesenchymal transition(EMT)is implicated in the metastatic process and presents a chal-lenge to epithelial cell adhesion molecule-based detection of circulating tumor cells(CTCs),which have been demon-strated to be a prognostic indicator in metastatic breast cancer.Although evidence has indicated that heterogeneity of CTCs based on EMT markers is associated with disease progression,no standard recommendations have been established for clinical practice.This study aimed to evaluate the prognostic significance of dynamic CTC detection based on EMT for metastatic breast cancer patients.Methods:We enrolled 108 human epidermal growth factor receptor 2-negative metastatic breast cancer patients from the prospective phase III CAMELLIA study and applied the CanPatrol CTC enrichment technique to identify CTC phenotypes(including epithelial CTCs,biphenotypic epithelial/mesenchymal CTCs,and mesenchymal CTCs)in peripheral blood samples.Receiver operating characteristic curve analyses of total CTC count and the proportion of mesenchymal CTCs for predicting the 1-year progression-free survival(PFS)rate were conducted to determine the optimal cut-off values,and Kaplan-Meier analysis and Cox proportional hazards regression analysis were performed to investigate the prognostic value of the cut-off values of both total CTC count and the proportion of mesenchymal CTCs in combination.Results:For predicting the 1-year PFS rate,the optimal cut-off value of total CTC count was 9.5(Area under the curve[AUC]=0.538,95%confidence interval[CI]=0.418-0.657),and that of the proportion of mesenchymal CTCs was 10.7%(AUC=0.581,95%CI=0.463-0.699).We used the two cut-off values in combination to forecast PFS in which the total CTC count was equaled to or exceeded 10/5 mL with the proportion of mesenchymal CTCs surpassed 10.7%.Patients who met the combined criteria had significantly shorter median PFS than did those who did not meet the criteria(6.2 vs.9.9 months,P=0.010).A nomogram was constructed based on the criteria and significant clinicopatho-logical characteristics with a C-index of 0.613(P=0.010).Conclusions: The criteria, which combine the total CTC count and the proportion of mesenchymal CTCs, may be used to monitor therapeutic resistance and predict prognosis in patients with metastatic breast cancer. | Xiuwen Guan Fei Ma Chunxiao Li Shiyang Wu Shangying Hu Jiefen Huang Xiaoying Sun Jiayu Wang Yang Luo Ruigang Cai Ying Fan Qiao Li Shanshan Chen Pin Zhang Qing Li Binghe Xu | 2019 | Cancer Communications2019，39，1： | 15 |
| 6 | Plasma resistin is increased in patients with unstable angina显示文摘背景 Resistin ,在啮齿类动物连接到胰岛素抵抗和肥胖的新奇 adipokine ,主要从巨噬细胞被导出并且在人在粉瘤识别了,被显示了在 atherosclerosis.Resistin 层次起一个潜在的作用被报导在冠的动脉疾病( CAD )增加,当有关在在中国人民的 CAD 的不同阶段的 resistin 的数据正在缺乏时。这研究的目的是估计 resistin 的血浆集中是否在病人之间不同与不稳定的,稳定的咽峡炎 pectoris.Methods 血浆 resistin 层次在有不稳定的咽峡炎( UAP )的 46 个病人借助于连接酶的 immunosorbent 试金( ELISA )是坚定的, 37 显著地在 UAP 组与稳定的咽峡炎(树液)和 resistin 的 31 控制 subjects.Results 血浆集中被增加(几何平均数( interquartile 范围) 12.09 ng/ml ( 8.40 , 18.08 ))与 S 比较在 resistin 层次的差别都没与树液和控制一起在病人之间被发现。我们也发现血浆 resistin 断然与白血球计数相关( r=0.21 , P=0.027 ),高敏感的C反应的蛋白质( hs-CRP )( r=0.25 , P=0.008 ),并且 endothelin-1 ( r=0.21 , P=0.025 )在调整以后好久,性和 BMI.Conclusion Resistin 可以被影响全身的发炎和 endothelial 激活涉及 CAD 的发展。 | HU Wen-lan QIAO Shu-bin HOU Qing YUAN Jian-song | 2007 | Chinese Medical Journal2007，，10： | 14 |
| 7 | Chemical and optical properties of aerosols and their interrelationship in winter in the megacity Shanghai of China显示文摘A field campaign on air quality was carried out in Shanghai in winter of 2012. The concentrations of NO, NO2, NOx, SO2, CO, and PM2.5increased during haze formation. The average masses of SO42-, NO3-and NH4+were 10.3, 11.7 and 6.7 μg/m3 during the haze episodes, which exceeded the average(9.2, 7.9, and 3.4 μg/m3) of these components in the non-haze days. The mean values for the aerosol scattering coefficient(b sp), aerosol absorption coefficient(b ap) and single scattering albedo(SSA) were 288.7, 27.7 and0.91 Mm-1, respectively. A bi-peak distribution was observed for the mass concentrations of CO, NO, NO2, and NOx. More sulfate was produced during daytime than that in the evening due to photochemical reactions. The mass concentration of NH4+achieved a small peak at noontime. NO3-showed lower concentrations in the afternoon and higher concentrations in the early morning. There were obvious bi-peak diurnal patterns for b sp and b ap as well as SSA. b sp and b ap showed a positive correlation with PM2.5mass concentration.(NH4)2SO4, NH4NO3, organic mass, elemental carbon and coarse mass accounted for 21.7%, 19.3%, 31.0%, 9.3% and 12.3% of the total extinction coefficient during non-haze days, and 25.6%, 24.3%, 30.1%, 8.1% and 8.2% during hazy days. Organic matter was the largest contributor to light extinction. The contribution proportions of ammonium sulfate and ammonium nitrate to light extinction were significantly higher during the hazy time than during the non-haze days. | Tingting Han Liping Qiao Min Zhou Yu Qu Jianfei Du Xingang Liu Shengrong Lou Changhong Chen Hongli Wang Fang Zhang Qing Yu Qiong Wu | 2015 | Journal of Environmental Sciences2015，27，1： | 14 |
| 8 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 9 | Allergy-related Evidences in Relation to Serum IgE:Data from the China State Key Laboratory of Respiratory Disease,2008-2013显示文摘Objective To investigate the serum total IgE(tlgE) and specific IgE(slgE) to common allergens among allergic patients in Guangzhou,China.Methods 7 085 patients were examined for tlgE and slgE to 15 allergens,based on the protocols of reversed enzyme allergosorbent test and the sandwich enzyme-linked immunosorbent assay.Results 3 758(53.04%) patients tested positive for tlgE,and 4 640(65.49%) for slgE.Der pteronyssinus,Der farinae,eggs,and cow's milk were the most common allergens leading to higher positive rates of slgE responses.Several peaks of sensitization were:Der pteronyssinus,Der farinae,and Blomia tropicalis at age 10-12;cow's milk at age below 3;eggs at age 4-6.The mean level and positive rate of tlgE tended to increase in subjects sensitized to more allergens.Sensitization to Der pteronyssinus(OR,1.6;P<0.05),Der farinae(OR,1.5;P<0.05),Blomia tropicalis(OR,1.4;P<0.05),Blattella germanica(OR,1.5;P<0.05),cow's milk(OR,1.3;P<0.05),and soy beans(OR,2.0;P<0.05) were independently correlated with allergy-related conditions in preliminary diagnosis.Conclusion The major allergens in Guangzhou include Der pteronyssinus,Der farinae,cow's milk,and eggs.Sensitization to these allergens appears to be predictors of allergy-related disorder. | SUN Bao Qing CHEN De Hui ZHENG Pei Yan HUANG Hui Min LUO Wen Ting ZENG Guang Qiao ZHANG Xiao Wen | 2014 | Biomedical and Environmental Sciences2014，27，7： | 11 |
| 10 | Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats显示文摘OBJECTIVE:To study the effect of Wuziyanzong treatment on the sperm quality and content of calcium ions(Ca 2+) in oligoasthenospermia rats.METHODS:A model of oligoasthenospermia was induced in 50 Sprague Dawley rats by treatment with tripterygium glycosides at 30 mg/kg per day for 8 weeks.They were divided randomly into a model group,a positive group(Huangjingzanyu capsule,3.01 g/kg),and low,medium and high dose Wuziyanzong treatment groups(2.30,4.60,9.20 g/kg crude drug respectively) with 10 in each group.Another 10 rats were used as a control group.The rats in the control and model groups were administered distilled water,while the rats in the remaining groups were administered Wuziyanzong for 30 d.The epididymides were removed,spermatozoa recovered and the sperm density and viability were measured.The spermatozoa were purified and the contents of Ca 2+ in the cytoplasm and mitochondria were detected by flow cytometry and atomic absorption spectrometry,respectively.RESULTS:After 8 weeks of treatment with tripterygium glycosides,the sperm density,sperm activity and the Ca 2 + content of spermatozoa in the model rats were all significantly decreased compared with the control group(all P<0.05).After 30 d treatment,the sperm density and activity improved and the Ca 2 + content of sperm were increased significantly in the medium and high dose Wuziyanzong treatment groups in comparison with the model group(all P<0.05).CONCLUSION:The Wuziyanzong treatment increased sperm density,improved sperm viability and enhanced the content of Ca 2+ in the sperm cytoplasm and mitochondria in this rat model of oligoasthenospermia. | Tongsheng Wang Jinling Huang Deling Wu Qing Li Xiangguo Liu Han Chen Lei Qiao Dongdong Li Jun Li | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 11 |
| 11 | Association Between Serum Uric Acid and Prevalence of Type 2 Diabetes Diagnosed using HbA1c Criteria Among Chinese Adults in Qingdao,China显示文摘Objective To determine whether elevated serum uric acid(UA)levels are associated with type 2 diabetes diagnosed using Hb A1 c levels among Chinese adults.Methods We conducted two population-based cross-sectional studies in Qingdao in China in 2006 and 2009.A total of 6894(39.4% men)subjects aged 35-74 years were included in the data analysis.Newly diagnosed diabetes was defined as Hb A1 c level of ≥6.5%,and prediabetes was classified as Hb A1 c level between 5.7% and 6.4% according to the International Diabetes Federation criteria.Multivariate logistic regression was employed to assess the association between UA and prevalence of type 2 diabetes defined using Glycated hemoglobin A1c(Hb A1 c levels.Results Subjects with prediabetes had higher UA levels than those with normal glucose tolerance,newly diagnosed diabetes,and known diabetes,with corresponding values of 325.1(82.5)μmol/L,310.9(84.2)μmol/L,291.3(81.7)μmol/L,305.2(83.6)μmol/L,respectively(P<0.001 for all comparisons).Binary logistic regression analysis showed that UA was a possible predictor for the prevalence of type 2 diabetes diagnosed using Hb A1 c levels,and the second quartile of UA levels had a higher odds ratio(OR:4.088;95% CI:2.900-5.765)for Hb A1 c than the other quartiles after adjusting for age,body mass index,sex,marital status,education,income,alcohol consumption,smoking,and cardiometabolic parameters.Conclusion Serum UA is significantly associated with type 2 diabetes diagnosed using Hb A1 c levels,independent of other cardiometabolic parameters. | XUE Bai TAN Ji Bin NING Feng SUN Jian Ping ZHANG Ke Yi LIU Li WANG Shao Jie ZHANG Dong Feng QIAO Qing PANG Zeng Chang | 2015 | Biomedical and Environmental Sciences2015，28，12： | 9 |
| 12 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 13 | Diabetes onset at an earlier age and high HbA1c levels as risk factors of diabetic retinopathy显示文摘AIM:To assess the effect of age at diabetes onset and uncontrollable high Hb A1 c levels on the development of diabetic retinopathy(DR)among Chinese type 2 diabetes mellitus(DM)patients.METHODS:This was a cross-sectional survey of diabetic patients in Subei district,China.Data covering physical measurements,fasting blood-glucose(FBG),glycosylated hemoglobin(Hb A1 c),blood lipid,urinary albumin/creatinine ratio(UACR),ocular fundus examination,and diabetes treatment records were collected.An independent sample t-test were used to analyze differences.A Logistic regression analysis was applied to study the independent risk factors of DR.RESULTS:A total of 1282 patients with type 2 DM were enrolled,and 191 cases had DR(14.9%).The age at diabetes onset,education level,alcohol consumption,Hb A1 c level,UACR level,and hypoglycemic drugs were independent influencing factors for DR.The older the onset of diabetes,the less likely to develop DR(OR:0.958,95%CI:0.942-0.975,P=0.000).Patients were then divided in terms of age at diabetes onset as follows:<50 y,50-59 y,60-69 y,and≥70 y.Compared with diabetes onset age<50 y,50-59 y(OR:0.463,95%CI:0.306-0.699,P=0.000),60-69 y(OR:0.329,95%CI:0.203-0.535,P=0.000)and≥70 y(OR:0.232,95%CI:0.094-0.577,P=0.002)were at a lower risk of DR.The prevalence of DR was highest in patients with diabetes onset age<50 y(29.5%,P<0.05).The Hb A1 c level(8.67±1.97)%and proportion of insulin injection(52.5%)in patients with diabetes onset<40 y were higher than in patients with older diabetes onset age(P<0.05).CONCLUSION:Diabetes onset at an earlier age and uncontrollable high Hb A1 c level could be independent risk factors for DR. | Rui-Fang Feng Hai-Yang Liu Ya-Lu Liu Qing Xu Lei Qiao Chao-Ju Gong Yi-Peng Zhang Jie Li Li-Na Guan Wei Fan Mei-Li Li Wen-Jin Li Su-Yan Li | 2021 | International Journal of Ophthalmology(English edition)2021，14，2： | 7 |
| 14 | How to make insulin-producing pancreatic β cells for diabetes treatment显示文摘Around 400 million people worldwide suffer from diabetes mellitus.The major pathological event for Type 1 diabetes and advanced Type 2 diabetes is loss or impairment of insulin-secreting β cells of the pancreas.For the past 100 years,daily insulin injection has served as a life-saving treatment for these patients.However,insulin injection often cannot achieve full glucose control,and over time poor glucose control leads to severe complications and mortality.As an alternative treatment,islet transplantation has been demonstrated to effectively maintain glucose homeostasis in diabetic patients,but its wide application is limited by the scarcity of donated islets.Therefore,it is important to define new strategies to obtain functional human β cells for transplantation therapies.Here,we summarize recent progress towards the production of β cells in vitro from pluripotent stem cells or somatic cell types including a cells,pancreatic exocrine cells,gastrointestinal stem cells,fibroblasts and hepatocytes.We also discuss novel methods for optimizing β cell transplantation and maintenance in vivo.From our perspective,the future of βcell replacement therapy is very promising although it is still challenging to control differentiation of β cells in vitro and to protect these cells from autoimmune attack in Type 1 diabetic patients.Overall,tremendous progress has been made in understanding βcell differentiation and producing functional β cells with different methods.In the coming years,we believe more clinical trials will be launched to move these technologies towards treatments to benefit diabetic patients. | Jiaqi Lu Qing Xia Qiao Zhou | 2017 | Science China(Life Sciences)2017，60，3： | 7 |
| 15 | Color-related chlorophyll and carotenoid concentrations of Chinese kale can be altered through CRISPR/Cas9 targeted editing of the carotenoid isomerase gene BoaCRTISO显示文摘The carotenoid isomerase gene(BoaCRTISO)of Chinese kale was targeted and edited using the CRISPR/Cas9 system in the present study.The results showed a high mutation rate(81.25%),and 13 crtiso mutants were obtained.Only two types of mutations,insertions and replacements,were found.Both the total and individual carotenoid and chlorophyll concentrations of the biallelic and homozygous mutants were reduced,and the total levels declined by 11.89–36.33%.The color of the biallelic and homozygous mutants changed from green to yellow,likely reflecting a reduction in the color-masking effect of chlorophyll on carotenoids.The expression levels of most carotenoid and chlorophyll biosynthesis-related genes,including CRTISO,were notably lower in the mutants than in the WT plants.In addition,the functional differences between members of this gene family were discussed.In summary,these findings indicate that CRISPR/Cas9 is a promising technique for the quality improvement of Chinese kale and other Brassica vegetables. | Bo Sun Min Jiang Hao Zheng Yue Jian Wen-Li Huang Qiao Yuan Ai-Hong Zheng Qing Chen Yun-Ting Zhang Yuan-Xiu Lin YanWang Xiao-Rong Wang Qiao-Mei Wang Fen Zhang Hao-Ru Tang | 2020 | Horticulture Research2020，7，1： | 7 |
| 16 | Trastuzumab plus adjuvant chemotherapy for human epidermal growth factor receptor 2(HER2)-positive early-stage breast cancer: A real-world retrospective study in Chinese patients显示文摘Objective: To assess the long-term effectiveness and safety of trastuzumab in adjuvant therapy for Chinese patients with early-stage human epidermal growth factor 2(HER2)-positive breast cancer in a real-world setting.Methods: This retrospective observational study analyzed the medical records of HER2-positive breast cancer patients between 2000 and 2012 at the Chinese Academy of Medical Sciences. Patients who received adjuvant chemotherapy alone or adjuvant chemotherapy followed by/combined with trastuzumab were included. The Kaplan-Meier method was used to estimate disease-free survival(DFS) and overall survival(OS). Hazard ratios(HR) and 95% confidence intervals(95% CI) were calculated using the Cox regression model.Results: Of the 1,348 patients analyzed, 909 received chemotherapy alone and 439 received chemotherapy plus trastuzumab. The 3-year, 5-year and 10-year DFS rates were 83.70%, 76.38% and 68.94%, respectively, in the chemotherapy-alone cohort, and 90.21%, 86.19% and 83.45% in the chemotherapy plus trastuzumab cohort. The3-year, 5-year and 10-year OS rates were 96.10%, 91.40% and 81.88% in the chemotherapy-alone cohort, and98.17%, 94.91% and 90.01% in the chemotherapy plus trastuzumab cohort. The chemotherapy plus trastuzumab group had a significantly lower risk of disease recurrence and death than the chemotherapy-alone group(DFS:HR=0.50, 95% CI, 0.37-0.68;P<0.001;OS: HR=0.53, 95% CI, 0.34-0.81;P=0.004) after adjusting for covariates.In the 439 patients treated with trastuzumab, multivariate analysis suggested that lymph node positivity, higher T stages, and hormone receptor-negative status were significantly associated with higher risks of disease recurrence,and lymph node positivity and hormone receptor-negative status were significantly associated with higher risks of death. Grade 3/4 adverse events(incidence ≥1%) were more common in patients receiving trastuzumab(54.44% vs.15.73%).Conclusions: Early-stage HER2-positive breast cancer patients treated with trastuzumab plus adjuvant chemotherapy have a significant survival benefit compared with chemotherapy-alone in real-world settings. Lymph node positivity, hormone receptor-negative status, and higher T stages may be associated with higher risks of recurrence, and effective therapy for patients with these factors is required. | Jihong Guo Qing Li Pin Zhang Peng Yuan Jiayu Wang Fei Ma Ying Fan Ruigang Cai Yang Luo Qiao Li Binghe Xu | 2019 | Chinese Journal of Cancer Research2019，31，5： | 6 |
| 17 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
| 18 | Prognostic Value of NT-proBNP in Stable Coronary Artery Disease in Chinese Patients after Percutaneous Coronary Intervention in the Drug-eluting Stent Era显示文摘Objective The predictive value of N-terminal pro-brain natriuretic peptide(NT-proBNP) in patients with stable coronary artery disease(SCAD) in the drug-eluting stent era is not yet clear. We aimed to evaluate the prognostic value of NT-proBNP in SCAD patients after percutaneous coronary intervention(PCI). Methods We examined 4,293 consecutive SCAD patients who underwent PCI between January 2013 and December 2013 in Fuwai Hospital, China. The primary endpoint was all-cause death. NT-proBNP levels were measured before PCI using Elisa kits(Biomedica, Austria). The indication for PCI was based on the degree of coronary stenosis and evidence of ischemia. Results Among 3,187 SCAD patients with NT-proBNP data, after a 2-year follow-up, NT-proBNP levels were predictive for all-cause death in the SCAD population [area under the receiver operating characteristic curve, 0.768; 95% confidence interval(CI), 0.687-0.849; P < 0.001]. At the optimum cutoff point of 732 pg/mL, the sensitivity and specificity of death was 75.0% and 72.3%, respectively. In a multivariable Cox regression model, the death hazard ratio was 6.43(95% CI, 2.99-13.82; P < 0.001) for patients with NT-proBNP levels ≥ 732 pg/mL, compared with < 732 pg/mL. Conclusion NT-proBNP is a strong predictor of 2-year death with SCAD after PCI in the drug-eluting stent era. | ZHAO Xue Yan LI Jian Xin TANG Xiao Fang XU Jing Jing SONG Ying JIANG Lin CHEN Jue SONG Lei GAO Li Jian GAO Zhan QIAO Shu Bin YANG Yue Jin GAO Run Lin XU Bo YUAN Jin Qing | 2018 | Biomedical and Environmental Sciences2018，31，12： | 5 |
| 19 | Anti-Hypertensive Action of Fenofibrate via UCP2 Upregulation Mediated by PPAR Activation in Baroreflex Afferent Pathway显示文摘Fenofibrate, an agonist for peroxisome proliferator-activated receptor alpha(PPAR-a), lowers blood pressure, but whether this action is mediated via baroreflex afferents has not been elucidated. In this study, the distribution of PPAR-a and PPAR-c was assessed in the nodose ganglion(NG) and the nucleus of the solitary tract(NTS). Hypertension induced by drinking high fructose(HFD) was reduced, along with complete restoration of impaired baroreceptor sensitivity, by chronic treatment with fenofibrate. The molecular data also showed that both PPAR-a and PPAR-c were dramatically up-regulated in the NG and NTS of the HFD group. Expression of the downstream signaling molecule of PPAR-a, the mitochondrial uncoupling protein 2(UCP2), was up-regulated in the baroreflex afferent pathway under similar experimental conditions, along with amelioration of reduced superoxide dismutase activity and increased superoxide in HFD rats.These results suggest that chronic treatment with fenofibrate plays a crucial role in the neural control of blood pressure by improving baroreflex afferent function due at least partially to PPAR-mediated up-regulation of UCP2 expression and reduction of oxidative stress. | Jian Guan Miao Zhao Chao He Xue Li Ying Li Jie Sun Wei Wang Ya-Li Cui Qing Zhang Bai-Yan Li Guo-Fen Qiao | 2019 | Neuroscience Bulletin2019，35，1： | 5 |
| 20 | Spinodal surface instability of soft elastic thin films显示文摘当薄电影的厚度归结为微米或甚至纳米时,表面精力和表面相互作用经常在他们的变丑行为和表面形态学起一个重要作用。瓦得瓦力完美地在一部软有弹性的薄电影导致的 spinodal 表面不稳定性结合了到僵硬底层用有弹性的结构的分叉理论理论上被调查。分析答案被财务为货车 der Waals 相互作用精力,有弹性的紧张精力和表面精力的比赛为 spinodal 表面形态学不稳定性的临界情况导出。表面精力,厚度和这部电影的有弹性的性质的效果上的详细考试证明变丑分叉模式的典型波长经由一种指数的关系取决于电影厚度,与在从 0.749 ~ 1.0 的范围的力量索引。理论答案与相关实验结果有一个好协议。 | Shi Qing Huang Xi Qiao Feng | 2008 | Acta Mechanica Sinica2008，24，3： | 5 |
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