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| 1 | Inhibitory effect of IGF-Ⅱ antisense RNA on malignant phenotype of hepatocellular carcinoma显示文摘INIRODUCTIONAccording to the therapeutic effect and strategy ofantisense RNA for hepatoccllular carcinoma(HCC),we have specifically synthesized partialcDNA of human insulin-like growth factor Ⅱ(IGF-Ⅱ)and constructed IGF-Ⅱ cDNA antisenseeukaryotic expression vector.The constructedvector was introduced into hepatoma cell lineSMMC-7721 to block the intrinsic IGF-Ⅱexpression.The biological behavior changes ofhepatoma cells were observed.All these | Dong Hua Yang Ming Qing Zhang Jiang Du Chong Xu Oiao Ming Liang Ji Fang Mao Han Rong Qin Zi Rong Fan Department of Gastroenterology,Zhujiang Hospital,the First Military Medical University,Guangzhou 510282,China Laboratory of Molecular Biology,Zhujiang Hospital,the First Military Medical University,Guangzhou,China Departrnent of Biochemistry,the Second Military Medical University,Shanghai,China | 2000 | World Journal of Gastroenterology2000，6，2： | 54 |
| 2 | Mitochondrial PKM2 regulates oxidative stress-induced apoptosis by stabilizing Bcl2显示文摘 | Ji Liang Ruixiu Cao Xiongjun Wang Yajuan Zhang Pan Wang Hong Gao Chen Li Fan Yang Rong Zeng Ping Wei Dawei Li Wenfeng Li Weiwei Yang | 2017 | Cell Research2017，27，3： | 39 |
| 3 | Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis显示文摘AIM:To perform a meta-analysis of observational studies and randomized controlled trials(RCTs)on the association between Helicobacter pylori(H.pylori)and iron deficiency anemia(IDA).METHODS:A defined search strategy was used to search Medline,Embase,the Cochrane Library,Clinical Trials,Cochrane Central Register of Controlled Trials,Premedline and Healthstar.Odds ratio(OR)was used to evaluate observational epidemiology studies,and weighted mean difference(WMD)was used to demonstrate the difference between control and intervention groups.RESULTS:Fifteen observational studies and 5 RCTs were identified and used for calculation.The pooled OR for observational studies was 2.22(95%CI:1.52-3.24,P<0.0001).The WMD for hemoglobin(HB) was 4.06 g/L(95%CI:-2.57-10.69,P=0.01),and the WMD for serum ferritin(SF)was 9.47μg/L(95%CI:-0.50-19.43,P<0.0001).Results were heterogeneous for all comparisons.CONCLUSION:This meta-analysis on observational studies suggests an association between H.pylori and IDA.In RCTs,eradication of H.pylori can improve HB and SF levels but not significantly. | Qu, Xin-Hua Huang, Xiao-Lu Xiong, Ping Zhu, Cui-Ying Huang, You-Liang Lu, Lun-Gen Sun, Xu Rong, Lan Zhong, Liang Sun, Da-Yu Lin, Hai Cai, Ming-Ci Chen, Zhi-Wei Hu, Bing Wu, Lian-Ming Jiang, Yi-Bin Yan, Wei-Li | 2010 | World Journal of Gastroenterology2010，16，7： | 34 |
| 4 | Possible early Neoproterozoic magmatism associated with slab window in the Pingshui segment of the Jiangshan-Shaoxing suture zone: Evidence from zircon LA-ICP-MS U-Pb geochronology and geochemistry显示文摘We report here geochemical data, U-Pb zircon ages, and Hf isotopes for the high-Mg diorites (HMDs), Nb-enriched basaltic porphyrys (NEBPs) and plagiogranites (PLAGs) in the Pingshui segment of the Jiangshan-Shaoxing suture zone. The HMDs are characterized by high Mg# (>60), high Na and LREE contents, depletion of HREE and HFSE, and pronounced positive εNd(t) values of 7.0 to 7.7, similar to some adakitic high-Mg andesites. The NEBPs are relatively Na-rich (Na2O/K2O>6) and display high abundances of P2O5 (~1.00%), TiO2 (~3.08%) and HFSE (e.g., Nb=9.53-10.27 ppm). Their Nd isotopic compositions (εNd(t)=6.8-8.0) are comparable to those of the HMDs. The PLAGs are metaluminous (A/CNK=0.84-0.89) and sodic (Na2O/K2O>10). Their depletion in HFSE (e.g., Nb, Ta) is consistent with 'SSZ-type' plagiogranite. Zircon LA-ICP-MS U-Pb dating yields an age of 932±7 Ma for the HMD, 916±6 Ma for the NEBP, and 902±5 Ma for the PLAG, respectively, indicating that they were products of early Neoproterozoic magmatism. The PLAGs exhibit relatively high zircon Hf isotopes and positive εHf(t) values of 11.0 to 16.2, consistent with their Nd isotopic data (εNd(t)=7.5-8.4). Such features are similar to those of oceanic plagiogranites in ophiolites and distinct from those of crust-derived granites. The PLAGs were most likely derived from partial melting of subducted oceanic crust in an active continental margin. Considering these results in the context of the regional geology, we suggest that a slab window in the subducting oceanic crust between the Yangtze Block and Cathaysia Block was possibly the principal cause for the unique arc magmatism in the area. The upwelling asthenosphere below the slab window may have provided significant thermodynamic conditions. | CHEN ZhiHong GUO KunYi DONG YongGuan CHEN Rong LI LongMing LIANG YiHong LI ChunHai YU XiMing ZHAO Ling XING GuangFu | 2009 | Science China Earth Sciences2009，52，7： | 26 |
| 5 | DESIGN METHODOLOGY OF NETWORKED SOFTWARE EVOLUTION GROWTH BASED ON SOFTWARE PATTERNS显示文摘最近,建筑群的新特征联网的一些在不同领域里吸引科学家的注意,并且导致许多种新兴的研究方向。到目前为止,大多数研究工作被结构分析在大规模软件系统在复杂网络特征的发现限制了。这篇论文论述理论基础,设计方法,算法和研究的实验结果。它第一为网络拓扑学强调进化生长的设计方法的意义面向对象(OO ) 软件系统,并且主张有各种各样的拓扑学特征的网络模型的选择和调整将在设计的进程和 OO 软件系统的实现上带不可忽略的效果。然后,我们与不同拓扑学特征和软件建模方法的发展分析“在网络模型的进化之间的否定和妥协的否定”的类似的纪律。根据软件模式的生长特征的分析,我们连续建议一个面向对象的软件网络进化生长方法和它的算法。另外,我们也基于复杂网络理论为 OO 软件系统度量标准建议参数系统。基于这些参数系统,它能分析各种各样的节点的特征,连接并且本地世界,调制网络拓扑学并且指导软件度量标准。所有这些能对详细设计,实现和表演分析有用。最后,我们集中于进化算法的应用程序并且由案例研究表明它。在实验软件工程从我们的早实验把结果与方法论作比较,我们相信建议软件工程设计方法基于复杂网络理论是一条计算软件工程途径。我们主张这个方法应该是极大地为在大规模 OO 的功能,结构和性能的设计,实现,调整和度量标准有益软件建筑群系统。 | Keqing HE Rong PENG Jing LIU Fei HE Peng LIANG Bing LI | 2006 | Journal of Systems Science & Complexity2006，19，2： | 24 |
| 6 | 电针和经皮穴位电刺激技术在生殖医学中的应用:专家共识(英文)显示文摘本文从传统针刺治疗不孕症的选穴依据与现代电针/经皮穴位电刺激技术的相关原理着手,从最优刺激参数选择、穴位辨证、疗程与治疗次数的确定及基本原理等方面阐述了电针/经皮穴位电刺激技术在多囊卵巢综合症、取卵镇痛、卵巢储备功能降低、胚胎移植以及男性少/弱精症等生殖领域中的具体应用。首次对电针/经皮穴位电刺激技术在生殖领域优势病种中的应用进行了系统的分析和总结,在最优刺激参数选择、穴位辨证、疗程与治疗次数的确定等方面提出了一系列专家共识,并为临床实践提供了指导意见和理论依据。 | Fan QU Rong LI Wei SUN Ge LIN Rong ZHANG Jing YANG Li TIAN Guo-gang XING Hui JIANG Fei GONG Xiao-yan LIANG Yan MENG Jia-yin LIU Li-ying ZHOU Shu-yu WANG Yan WU Yi-jing HE Jia-yu YE Song-ping HAN Ji-sheng HAN | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 24 |
| 7 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 8 | Application of nonlinear chemical fingerprinting to identification,evaluation and clinical use of Glycyrrhiza显示文摘Nonlinear chemical fingerprinting is a new technique for analyzing traditional Chinese medicines (TCMs). We developed such a method and applied it to Glycyrrhiza, a significant TCM. Nonlinear chemical fingerprints were obtained for Glycyrrhiza from four different production areas, and for 14 other TCMs with similar induction time. The calculation of their similarities was based on similarity system theory. Using intuitive visual comparison of the data and the similarity calculation results, Glycyrrhiza was discriminated from the 14 other TCMs. There was a linear relationship between the content of active TCM components and the induction time. This was used to compare the content of active components in Glycyrrhiza from the four different production areas, and establish an equivalent-dose relationship for these four samples. This method provides a new tool for discrimination and evaluation of Glycyrrhiza, and a dosage reference for clinical application of Glycyrrhiza from the four regions studied. | FANG XuanQi ZHANG TaiMing ZHAO Zhe XIANG FengQin LIANG YiZeng WANG Mei ZHU Rong CHEN Su QIAO JunXi | 2010 | Chinese Science Bulletin2010，55，26： | 20 |
| 9 | An alternative therapy for drug-resistant epilepsy: transcutaneous auricular vagus nerve stimulation显示文摘 | Rong Peijing Liu Aihua Zhang Jianguo Wang Yuping Yang Anchao Li Liang Ben Hui Li Liping Liu Rupeng He Wei Liu Huanguang Huang Feng Li Xia Wu Peng Zhu Bing | 2014 | Chinese Medical Journal2014，，2： | 20 |
| 10 | Proteomic characteristics of the liver and skeletal muscle in the Chinese tree shrew(Tupaia belangeri chinensis)显示文摘Valid animal models are useful for studying the pathophysiology of specific disorders,such as neural disease,diabetes and cancer.Previous molecular phylogeny studies indicate that the tree shrew is in the same order as(or a close sister to)primates,and thus may be an ideal model in which to study human disease.In this study,the proteome of liver and muscle tissue in tree the shrew was identified by combining peptide fractionation and LC-MS/MS identification.In total,2146 proteins were detected,including 1759 proteins in liver samples and 885 proteins in skeletal muscle samples from the tree shrew.Further sub-source analysis revealed that nearly half of the identified proteins(846 proteins and 418 proteins)were derived from human database.In this study,we are the first to describe the characteristics of the proteome from the liver and skeletal muscle of the tree shrew.Phylogenetic tree analysis based on these proteomic data showed that the tree shrew is closer to primates(human)than to glires(the mouse and rat). | Rongxia Li Wei Xu Zhen Wang Bin Liang Jia-Rui Wu Rong Zeng | 2012 | Protein & Cell2012，3，9： | 18 |
| 11 | Determining method,conditional factors,traits and applications of nonlinear chemical fingerprint by using dissipative components in samples显示文摘The thermodynamic systems and dynamic model suitable for determining the nonlinear chemical fingerprints of samples were analyzed.The results indicated that the damp nonlinear chemical reactions in close systems away from the equilibrium and open systems without the complementarity of the dissipation substances have important significance for the throng characterization and whole content analysis of chemical components in samples.Various factors influencing on nonlinear chemical fingerprint,such as reactant species and their concentrations,electrode types,temperature,stir rate,the sort,dosage and granularity of the sample,etc.were amply researched by a nonlinear chemistry reaction,namely,damp B-Z oscillation which used acetone and glucose as the main dissipative substances.In addition,the quantitative information on the whole of chemical components in samples and the traits and applications of the fingerprint were investigated.The method and its important conditions for determining nonlinear chemistry fingerprint used in distinguishing and evaluating complex samples were successfully put forward. | ZHANG TaiMing ZHAO Zhe FANG XuanQi QIAO JunXi XIANG FengQin ZHU Rong LIANG YiZeng DING Feng | 2012 | Science China Chemistry2012，55，2： | 16 |
| 12 | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats显示文摘Objective To explore the Intervention effect of Rosiglitozone in ovarian fibrosis of PCOS rats.Methods 60 female SD rats were randomly divided into 3 groups:control group,model group and treatment group.The model and treatment groups were established by subcutaneous injection of DHEA,while the treatment group was given RGZ.The serum hormone values,pathohistology of ovarian structure of rats,ovarian ultrastructure and the expressions of TGF-β 1 and CTGF were detected.Results The PCOS model was established successfully.The expression intensity of TGF-β 1 and CTGF in Oocytes of the PCOS groups was 9.545±2.954 and 9.665±2.400,respectively and was significantly higher than that of the control group 6.636±2.264 and 7.036±2.133;after treatment with rosiglitazone,the expression was significantly decreased 6.980±2.421 and 6.642±2.721 as compared with that of the model group (P<0.05,P<0.001).The values in serum of the PCOS groups were 3.749±2.054 and 0.265±0.129,and 1.914±1.801 and 0.096±0.088 in the control group which had statistically significant difference (P<0.05,P<0.001).After treatment with rosiglitazone,the values were 2.3100±1.825 and 0.112±0.187 and were significantly different with those of the model group (P<0.05,P<0.001).Conclusion TGF-β 1 and CTGF play an important role in the development of ovary fibrosis in PCOS.However,RGZ may postpone the development of fibrosis by decreasing the levels of TGF-β 1 and CTGF. | MIAO Zhu Lin GUO Liang WANG Yong Xia CUI Rong YANG Ning HUANG Mi Qiong QIN Wei Bing CHEN Jin LI Hong Mei WANG Zi Neng WEI Xiang Cai | 2012 | Biomedical and Environmental Sciences2012，25，1： | 14 |
| 13 | Principle of nonlinear chemical fingerprint by using dissipative components in samples as well as calculation and evaluation of similarity显示文摘Under the conditions of constant temperature and pressure,different influences of samples with different chemical components on the mechanism of nonlinear chemical reaction will cause different changes of the potential-time relationship curve of the nonlinear chemical reaction system.Using it as the character,and using the B-Z nonlinear chemical system to use acetone and substrates in samples as main dissipative substances qua an example,the principle of nonlinear chemical fingerprint has been researched and discussed in detail.At the same time,the general method for calculating the system similarity about nonlinear chemical fingerprint was also put forward,and similarities of nonlinear chemistry fingerprints of different batches of Guhan Yangshengjing and 18 sorts of other samples were calculated by Euclidean distance,correlation coefficient,included angle cosine and system similarity,at the same time,the various similarities were analyzed.The results showed that,both of correlation coefficient and included angle cosine are unable to be used as the criterion for quantitatively evaluating the similarity of nonlinear chemistry fingerprint;as non-parametric similarity,Euclidean distance can accurately reflect the feature differences in the fingerprints,but as parametric similarity,sometimes,Euclidean distance can not accurately reflect the relative extent of characteristic difference in the nonlinear chemical fingerprints;system similarity can most truthfully reflect the characteristic difference in the nonlinear chemical fingerprints,and is the best evaluating method among the four ones.Therefore,system similarity can be used to quantitatively calculate the similar extent between the nonlinear chemical fingerprints.An economical,simple and convenient,easy pushing and effective method for identifying and evaluating complicated samples has successfully been put forward. | ZHANG TaiMing ZHAO Zhe FANG XuanQi QIAO JunXi XIANG FengQin ZHU Rong LIANG YiZeng DING Feng | 2012 | Science China Chemistry2012，55，2： | 13 |
| 14 | Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway显示文摘In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P<0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P<0.05),while BMP-2 was not (P>0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P<0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P<0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P>0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein. | Fang LIU Hui ZHONG Jing-yuan LIANG Ping FU Zhi-juan LUO Li ZHOU Rong GOU Jun HUANG | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，12： | 12 |
| 15 | Effect of acupuncture at Neiguan (PC 6) on cardiac function using echocardiography in myocardial ischemia rats induced by isoproterenol显示文摘OBJECTIVE: To investigate the effect of acupuncture at Neiguan(PC 6) on cardiac function using echocardiography in rat models of myocardial ischemia(MI) induced by isoproterenol(ISO).METHODS: Twenty-seven Sprague-Dawley rats were randomly assigned to normal, model, and acupuncture groups. The model and acupuncture groups were given injections of ISO(85 mg/kg) to establish the MI model. After model establishment,the acupuncture group was treated with acupuncture at Neiguan(PC 6) for 30 min. Echocardiography was used to monitor diastolic and systolic function for 30 min starting from the time after the acupuncture needles were removed. Changes in the length of left ventricular internal diameter at end-diastole(LVIDd), length of left ventricular internal diameter at end-systole(LVIDs), the ratio of mitral peak velocity at early diastole and atrial contraction(E/A), ejection fraction(EF), fractional shortening(FS), and stroke volume(SV) were measured.RESULTS: Compared with the model group at 0and 15 min after needles were removed, the means of LVIDd and LVIDs were significantly lower(P <0.01) and E/A, EF, FS, and SV significantly higher(P < 0.01) in the acupuncture group. In the acupuncture group, the means of LVIDd and LVIDs15 min after the needles were removed were significantly higher(P < 0.01) than those at 0 min. The means of E/A, EF, FS, and SV significantly decreased(P < 0.01) from 0 to 15 min in the acupuncture group.CONCLUSION: These findings indicate that acupuncture at Neiguan(PC 6) can affect cardiac function by increasing left ventricular diastolic and systolic function in MI rat models, but the effect only lasts for 15 min. | Wang Songzi Ren Lu Jia Lianqun Gu Zhongyue Fan Xu Chen Wenna Hou Ping Liang Fanrong Rong Peijing Ma Tieming Liu Xulai Chen Yiguo | 2015 | Journal of Traditional Chinese Medicine2015，35，6： | 12 |
| 16 | Equivalent thermal resistance minimization for a circular disc heat sink with reverting microchannels based on constructal theory and entransy theory显示文摘Thermal designs for microchannel heat sinks with laminar flow are conducted numerically by combining constructal theory and entransy theory. Three types of 3-D circular disc heat sink models, i.e. without collection microchannels, with center collection microchannels, and with edge collection microchannels, are established respectively. Compared with the entransy equivalent thermal resistances of circular disc heat sink without collection microchannels and circular disc heat sink with edge collection microchannels, that of circular disc heat sink with center collection microchannels is the minimum, so the overall heat transfer performance of circular disc heat sink with center collection microchannels has obvious advantages. Furthermore, the effects of microchannel branch number on maximum thermal resistance and entransy equivalent thermal resistance of circular disc heat sink with center collection microchannels are investigated under different mass flow rates and heat fluxes. With the mass flow rate increasing, both the maximum thermal resistances and the entransy equivalent thermal resistances of heat sinks with respective fixed microchannel branch number all gradually decrease. With the heat flux increasing, the maximum thermal resistances and the entransy equivalent thermal resistances of heat sinks with respective fixed microchannel branch number remain almost unchanged. With the same mass flow rate and heat flux, the larger the microchannel branch number, the smaller the maximum thermal resistance. While the optimal microchannel branch number corresponding to minimum entransy equivalent thermal resistance is 6. | WANG Liang XIE ZhiHui CHEN LinGen WANG Rong FENG HuiJun | 2021 | Science China(Technological Sciences)2021，64，1： | 11 |
| 17 | Microstructural evolution and stress state related to mechanical properties of electron beam melted Ti-6Al-4V alloy modified by laser shock peening显示文摘This work characterizes microstructural evolutions of electron beam melted(EBM) Ti-6 Al-4 V alloy modified via laser shock peening(LSP).The depth stress distribution and tensile properties of EBM Ti-6 Al-4 V alloy were measured before and after LSP.The results indicate that microstructure consists of β phase with 7.2%±0.4% vol.% and balance α lamellar in EBM sample,and the α lamella was refined into nano-equiaxed grains and submicro-equiaxed grains after LSP.The dominant refinement mechanism is revealed during LSP.Stacking faults were found in the LSP-treated sample,and their corresponding planes were determined as(0001) basal plane,(1010) prismatic plane,and(1011) pyramidal plane obtained by high resolution transmission electron microscopy.The subgrains and high-angle grains formed during dynamic recrystallization were identified by selected area electron diffraction pattern.The LSP treatment produces a significantly residual compressive stress approximately-380 MPa with the depth of compressive stress layer reaching 450 μm.Strength and elongation of the EBM sample were significantly increased after LSP.The strength and ductility enhancements are attributed to compre s sive stress,grain refinement and grain gradient distribution of α phase. | Liang Lan Xinyuan Jin Shuang Gao Bo He Yonghua Rong | 2020 | Journal of Materials Science & Technology2020，47，15： | 11 |
| 18 | DNA barcoding of 18 species of Bovidae显示文摘Genetic divergences of mitochondrial cytochrome c oxidase subunit I genes,known as DNA barcodes,have been used in species identification in the animal kingdom.Barcodes can assist field workers and taxonomists to determine groups in need of taxa analysis,and facilitate the recognition of appropriate populations and scales for conservation planning.In this study,18 species of Bovidae were selected to evaluate the effectiveness of DNA barcoding for species differentiation.The results showed that all but 2 species had unique DNA barcodes.The mean intraspecific variation was 0.63%,yielding a threshold of 6.3% for flagging putative species.The results supported the inference that barcode variation within species of mammals is somewhat higher than within other animal groups.The present study validated the effectiveness of barcoding for the identification of bovid species. | CAI YanSen ZHANG Liang SHEN FuJun ZHANG WenPing HOU Rong YUE BiSong LI Jing ZHANG ZhiHe | 2011 | Chinese Science Bulletin2011，56，2： | 10 |
| 19 | Keyhole approach surgery for petroclival meningioma显示文摘在中国的背景,在 petroclival meningioma 的外科的处理的锁眼途径的可行性很好没被评估。这份报告在 25 总结了我们的经验与锁眼被对待的有 petroclival meningioma 的病人走近外科。从 2000 年 7 月的方法到 2005 年 7 月,有 petroclival meningioma 的 25 个病人经由潜水艇受到切除术时间,制动火箭 S 字形或联合的锁眼途径。肿瘤切除术的程度在外科,和手术后的复杂并发症以后到 MRI 3 月被评估我们 reinvestigated。结果肿瘤的最大的直径从 2 ~ 7 厘米(平均数, 4.5 厘米) 。Grosstotal 切除术(GTR ) 在 14 个病人被完成,给 56% 的 GTR 率。小计切除术(STR ) 在 3 在 8 个病人和部分切除术被执行。而其它受不了头部的神经赤字(Ⅶ,Ⅶ,Ⅲ和更低的 CN ) , 13 个病人使神经病学的地位正常。一个病人在手术后的时期死了。结论锁眼途径外科,特别联合锁眼途径对 petroclival meningioma 的治疗合适。它提供容易、快的存取给在上 -- 并且没有岩石骨头钻的 infratentorial juxta-clival 区域。与途径有关的复杂并发症能被最小化。 | ZHU Wei MAO Ying ZHOU Liang-fu ZHANG Rong CHEN Liang | 2006 | Chinese Medical Journal2006，，16： | 9 |
| 20 | Isothermal Microcalorimetry:A Review of Applications in Soil and Environmental Sciences显示文摘Isothermal microcalorimetry provides thermodynamic and kinetic information on various reactions and processes and is thereby a powerful tool to elucidate their mechanisms. Certain improvement in isothermal microcalorimetry with regard to the studies on soil and environmental sciences is briefly described. This review mainly focuses on the use of microcalorimetry in the determination of soil microbial activity, monitoring the toxicity and biodegradation of soil organic pollutants, the risk evaluation of metals and metalloids, the heat effect of ion exchange and adsorption in soil, and environmental researches. Promising prospects for the applications of the technique in the field are also discussed. | RONG Xing-Min HUANG Qiao-Yun JIANG Dai-Hua CAI Peng LIANG Wei | 2007 | Pedosphere2007，17，2： | 9 |
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