|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China显示文摘To control the spread of the 2019 novel coronavirus(COVID-19),China imposed nationwide restrictions on the movement of its population(lockdown)after the Chinese New Year of 2020,leading to large reductions in economic activities and associated emissions.Despite such large decreases in primary pollution,there were nonetheless several periods of heavy haze pollution in eastern China,raising questions about the well-established relationship between human activities and air quality.Here,using comprehensive measurements and modeling,we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution.In particular,large decreases in NOx emissions from transportation increased ozone and nighttime NO_(3)radical formation,and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter.Our results,afforded by the tragic natural experiment of the COVID-19 pandemic,indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants. | Xin Huang Aijun Ding Jian Gao Bo Zheng Derong Zhou Ximeng Qi Rong Tang Jiaping Wang Chuanhua Ren Wei Nie Xuguang Chi Zheng Xu Liangduo Chen Yuanyuan Li Fei Che Nini Pang Haikun Wang Dan Tong Wei Qin Wei Cheng Weijing Liu Qingyan Fu Baoxian Liu Fahe Chai Steven JDavis Qiang Zhang Kebin He | 2021 | National Science Review2021，8，2： | 47 |
| 2 | Changes in Mean and Extreme Temperature and Precipitation over the Arid Region of Northwestern China: Observation and Projection显示文摘This paper reports a comprehensive study on the observed and projected spatiotemporal changes in mean and extreme climate over the arid region of northwestern China, based on gridded observation data and CMIP5 simulations under the RCP4.5 and RCP8.5 scenarios. The observational results reveal an increase in annual mean temperature since 1961, largely attributable to the increase in minimum temperature. The annual mean precipitation also exhibits a significant increasing tendency. The precipitation amount in the most recent decade was greater than in any preceding decade since 1961. Seasonally,the greatest increase in temperature and precipitation appears in winter and in summer, respectively. Widespread significant changes in temperature-related extremes are consistent with warming, with decreases in cold extremes and increases in warm extremes. The warming of the coldest night is greater than that of the warmest day, and changes in cold and warm nights are more evident than for cold and warm days. Extreme precipitation and wet days exhibit an increasing trend, and the maximum number of consecutive dry days shows a tendency toward shorter duration. Multi-model ensemble mean projections indicate an overall continual increase in temperature and precipitation during the 21 st century. Decreases in cold extremes, increases in warm extremes, intensification of extreme precipitation, increases in wet days, and decreases in consecutive dry days, are expected under both emissions scenarios, with larger changes corresponding to stronger radiative forcing. | Yujie WANG Botao ZHOU Dahe QIN Jia WU Rong GAO Lianchun SONG | 2017 | Advances in Atmospheric Sciences2017，34，3： | 37 |
| 3 | Mitochondrial PKM2 regulates oxidative stress-induced apoptosis by stabilizing Bcl2显示文摘 | Ji Liang Ruixiu Cao Xiongjun Wang Yajuan Zhang Pan Wang Hong Gao Chen Li Fan Yang Rong Zeng Ping Wei Dawei Li Wenfeng Li Weiwei Yang | 2017 | Cell Research2017，27，3： | 39 |
| 4 | China National Medical Products Administration approval summary:anlotinib for the treatment of advanced non-small cell lung cancer after two lines of chemotherapy显示文摘Background:On May 8,2018,the China National Medical Products Administration(NMPA)approved anlotinib,an orally administered anti-angiogenesis inhibitor,for the treatment of patients with advanced non-small cell lung can-cer(NSCLC)who have progressed after treatment with two or more lines of prior systemic chemotherapy.Main body of the abstract:China NMPA reviewed and inspected a regional double-blinded,placebo-controlled,Phase III trial comparing the overall survival(OS)of NSCLC patients between the anlotinib and placebo arms.A total of 437 patients were randomized(2:1)to receive either anlotinib(n=294)or placebo(n=143)once daily on a 2-week on and 1-week off schedule.Patients with epidermal growth factor receptor(EGFR)or activating anaplastic lymphoma kinase(ALK)genomic tumor aberrations should have disease progression on NMPA-approved therapy.Anlotinib is the first NMPA-approved drug for patients with advanced NSCLC who have progressed on at least two lines of prior systemic chemotherapies in China.The approval was based on a statistically and clinically significant improvement in median OS with anlotinib(9.46 months)compared with placebo[6.37 months;hazard ratio(HR])=0.70,95%confidence interval(CI)=0.55-0.89;two-sided log-rank P=0.002].The confirmed objective response rate(ORR)was 9.2%in the anlotinib arm and 0.7%in the placebo arm.The median duration of response(DoR)was 4.83 months,with a 95%CI of 3.31-6.97 months.The toxicity profile of anlotinib was consistent with that of known anti-angiogenesis inhibitors.Common adverse drug reactions(ADRs)in anlotinib-treated patients included hypertension(67.4%),hand-foot syndrome(43.9%),hemoptysis(14.0%),thyroid stimulating hormone(TSH)elevation(46.6%),and corrected QT interval(QTc)prolongation(26.2%).Short conclusion:Anlotinib demonstrated a clinically significant OS prolongation as a novel therapeutic option for advanced or metastatic NSCLC following at least two lines of chemotherapy. | Ming Zhou Xiaoyuan Chen Hong Zhang Lin Xia Xin Tong Limin Zou Ruimin Hao Jianhong Pan Xiao Zhao Dongmei Chen Yuanyuan Song Yueli Qi Ling Tang Zhifang Liu Rong Gao Yuankai Shi Zhimin Yang | 2019 | Cancer Communications2019，39，1： | 35 |
| 5 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 6 | Telomerase activity in gastric cancer and its clinical implications显示文摘INTRODUCTIONThedevelopmentofcarcinomaresultsfrommultipleindependentgeneticchangesthatactivateprotooncogenesorinactivatetheac... | ZHAN Wen Hua, MA Jin Ping, PENG Jun Sheng, GAO Jing Song, CAI Shi Rong, WANG Jian Ping, ZHENG Zhang Qing and WANG Lei | 1999 | World Journal of Gastroenterology1999，5，4： | 25 |
| 7 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 8 | Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China显示文摘 | LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng | 2011 | Chinese Medical Journal2011，，5： | 21 |
| 9 | Microwave synthesis of Li_2FeSiO_4 cathode materials for lithium-ion batteries显示文摘A novel synthetic method of microwave processing to prepare Li_2FeSiO_4 cathode materials is adopted.The Li_2FeSiO_4 cathode material is prepared by mechanical ball-milling and subsequent microwave processing.Olivin-type Li_2FeSiO_4 sample with uniform and fine particle sizes is successfully and fast synthesized by microwave heating at 700℃in 12 min.And the obtained Li_2FeSiO_4 materials show better electrochemical performance and microstructure than those of Li_2FeSiO_4 sample by the conventional solidstate reaction. | Zhong Dong Peng Yan Bing Cao Guo Rong Hu Ke Du Xu Guang Gao Zheng Wei Xiao | 2009 | Chinese Chemical Letters2009，20，8： | 20 |
| 10 | The endoplasmic reticulum stress inhibitor salubrinal inhibits the activation of autophagy and neuroprotection induced by brain ischemic preconditioning显示文摘 | Bo GAO Xiang-yang ZHANG Rong HAN Tong-tong ZHANG Cheng CHEN Zheng-hong QIN Rui SHENG | 2013 | Acta Pharmacologica Sinica2013，34，5： | 19 |
| 11 | Manipulating mRNA splicing by base editing in plants显示文摘Precursor-mRNAs(pre-mRNA) can be processed into one or more mature m RNA isoforms through constitutive or alternative splicing pathways. Constitutive splicing of pre-mRNA plays critical roles in gene expressional regulation, such as intronmediated enhancement(IME), whereas alternative splicing(AS) dramatically increases the protein diversity and gene functional regulation. However, the unavailability of mutants for individual spliced isoforms in plants has been a major limitation in studying the function of mRNA splicing. Here, we describe an efficient tool for manipulating the splicing of plant genes. Using a Cas9-directed base editor, we converted the 5′ splice sites in four Arabidopsis genes from the activated GT form to the inactive AT form. Silencing the AS of HAB 1.1(encoding a type 2 C phosphatase) validated its function in abscisic acid signaling, while perturbing the AS of RS31 A revealed its functional involvement in plant response to genotoxic treatment for the first time. Lastly,altering the constitutive splicing of Act2 via base editing facilitated the analysis of IME. This strategy provides an efficient tool for investigating the function and regulation of gene splicing in plants and other eukaryotes. | Chenxiao Xue Huawei Zhang Qiupeng Lin Rong Fan Caixia Gao | 2018 | Science China(Life Sciences)2018，61，11： | 18 |
| 12 | Evaluation of preoperative staging for esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC) is known for its rapid progression and poor outcomes. China has the highest incidence and mortality in the world. Diagnoses made at early stages and accurate staging are associated with better outcomes, all of which can play a significant role in the selection of treatment protocols. ESCC is staged according to the widely accepted TNM system. Common imaging modalities used in staging ESCC before treatment include endoscopy, computed tomography(CT), positron emission tomography(PET) and magnetic resonance imaging(MRI). Endoscopic ultrasound is useful for staging tumor depth and nodal status. Narrow band imaging is valuable for early stage disease assessment. CT and PET provide additional valuable information regarding node and metastasis staging. The ability of MRI to delineate ESCC is continuously being improved and adds information regarding locoregional status to routine examinations. | Lin-Na Luo Long-Jun He Xiao-Yan Gao Xin-Xin Huang Hong-Bo Shan Guang-Yu Luo Yin Li Shi-Yong Lin Guo-Bao Wang Rong Zhang Guo-Liang Xu Jian-Jun Li | 2016 | World Journal of Gastroenterology2016，22，29： | 18 |
| 13 | Incidence of gastroesophageal reflux disease in Uygur and Han Chinese adults in Urumqi显示文摘AIM:To investigate the incidence of gastroesophageal reflux disease(GERD) and its related risk factors in Uygur and Han Chinese adult in Urumqi,China.METHODS:A population-based cross-sectional survey was undertaken in a total of 972 Uygur(684 male and 288 female) aged from 24 to 61 and 1023 Han Chinese(752 male and 271 female) aged from 23 to 63 years.All participants were recruited from the residents who visited hospital for health examination from November 2011 to May 2012.Each participant signed an informed consent and completed a GERD questionnaire(GerdQ) and a lifestyle-food frequency questionnaire survey.Participants whose Gerd Q score was ≥ 8 and met one of the following requirements would be enrolled into this research:(1) being diagnosed with erosive esophagitis(EE) or Barrett's esophagus(BE) by endoscopy;(2) negative manifestation under endoscopy(non-erosive reflux disease,NERD) with abnormal acid reflux revealed by 24-h esophageal pH monitoring;and(3) suffering from typical heartburn and regurgitation with positive result of proton pump inhibitor test.RESULTS:According to Gerd Q scoring criteria,340 cases of Uygur and 286 cases of Han Chinese were defined as GERD.GERD incidence in Uygur was significantly higher than in Han Chinese(35% vs 28%,χ2 = 11.09,P < 0.005),Gerd Q score in Uygur was higher than in Han Chinese(7.85 ± 3.1 vs 7.15 ± 2.9,P < 0.005),and Gerd Q total score in Uygur male was higher than in female(8.15 ± 2.8 vs 6.85 ± 2.5,P < 0.005).According to normalized methods,304(31%) cases of Uygur were diagnosed with GERD,including 89 cases of EE,185 cases of NERD and 30 cases of BE;256(25%) cases of Han Chinese were diagnosed with GERD,including 90 cases of EE,140 cases of NERD and 26 cases of BE.GERD incidence in Uygur was significantly higher than in Han Chinese(31% vs 25%,χ2 = 9.34,P < 0.005) while the incidences were higher in males of both groups than in females(26% vs 5% in Uygur,χ2 = 35.95,P < 0.005,and 19.8% vs 5.2% in Han,χ2 = 5.48,P < 0.025).GERD incidence in Uygur male was higher than in Han Chinese male(26% vs 19.8%,χ2 = 16.51,P < 0.005),and incidence of NERD in Uygur was higher than in Han Chinese(χ2 = 10.06,P < 0.005).Occupation(r = 0.623),gender(r = 0.839),smoking(r = 0.322),strong tea(r = 0.658),alcohol drinking(r = 0.696),meat-based diet(mainly meat)(r = 0.676) and body mass index(BMI)(r = 0.567) were linearly correlated with GERD in Uygur(r = 0.833,P = 0.000);while gender(r = 0.957),age(r = 0.016),occupation(r = 0.482),strong tea(r = 1.124),alcohol drinking(r = 0.558),meat diet(r = 0.591) and BMI(r = 0.246) were linearly correlated with GERD in Han Chinese(r = 0.786,P = 0.01).There was no significant difference between Gerd Q scoring and three normalized methods for the diagnosis of GERD.CONCLUSION:GERD is highly prevalent in adult in Urumqi,especially in Uygur.Male,civil servant,smoking,strong tea,alcohol drinking,meat diet and BMI are risk factors correlated to GERD. | Chun-Yan Niu Yong-Li Zhou Rong Yan Ni-La Mu Bao-Hua Gao Fang-Xiong Wu Jin-Yan Luo | 2012 | World Journal of Gastroenterology2012，18，48： | 17 |
| 14 | Changes of wetland landscape patterns in Dadu River catchment from 1985 to 2000, China显示文摘基于在 1985 和 2000 遥感图象处理的解释和向量,在在最后二十年的 Dadu 河集水的沼泽地风景模式的空间变化用空间分析被学习方法。由 Apack 软件支持了,沼泽地的索引美化模式被计算,并且信息熵(IE ) 是也介绍了证明沼泽地的变化美化信息。结果证明在这个区域的那处沼泽地风景 patch-corridor-matrix 配置是特征的并且主导地由自然沼泽地组成了。风景模式随着低碎片有点变化了并且从 1985 ~ 2000 在时期期间与部分散布分发显示出集中的分发。使稳定的补丁密度和集中索引的值,和差异,平均索引和 IE 的值显示出细微减少,当优势和分数维的尺寸索引被增加时。沼泽地风景的所有类型在 1985 和 2000 与草地风景有更高的毗邻可能性,并且由于低毗邻矩阵价值在河,冰河,水库和池溏沼泽地之中有极其弱的水文学连接和大空间差距。因为他们的累积贡献通过 PCA 分析超过了 81% ,农业活动将在过去的 15 年期间是到风景变化的主要驱动力[出版摘要] | Laibin HUANG Junhong BAI Denghua YAN Bin CHEN Rong XIAO Haifeng GAO | 2012 | Frontiers of Earth Science2012，6，3： | 17 |
| 15 | Primary intestinal non-Hodgkin's lymphoma:A clinicopathologic analysis of 81 patients显示文摘AIM:To analyze the clinicopathologic features and the prognosis of primary intestinal lymphoma.METHODS:Patients were included in the study based on standard diagnostic criteria for primary gastrointesti-nal lymphoma,and were treated at Sun Yat-sen Univer-sity Cancer Centre between 1993 and 2008.RESULTS:The study comprised 81 adults.The most common site was the ileocaecal region.Twenty-two point two percent patients had low-grade B-cell lym-phoma.Fifty-one point nine percent patients had high-grade B-cell lymphoma and 25.9% patients had T-cell lymphoma.Most patients had localized disease.There were more patients and more early stage diseases in the latter period,and the origin sites changed.The ma-jority of patients received the combined treatment,andabout 20% patients only received nonsurgical therapy.The wverall survival and event-free survival rates after 5 years were 71.6% and 60.9% respectively.The mul-tivariate analysis revealed that small intestine and ileo-caecal region localization,B-cell phenotype,and normal lactate dehydrogenase were independent prognostic factors for better patient survival.Surgery based treat-ment did not improve the survival rate.CONCLUSION:Refined stratification of the patients according to the prognostic variables may allow indi-vidualized treatment.Conservative treatment may be an optimal therapeutic modality for selected patients. | Guo-Bao Wang Guo-Liang Xu Guang-Yu Luo Hong-Bo Shan Yin Li Xiao-Yan Gao Jian-Jun Li Rong Zhang | 2011 | World Journal of Gastroenterology2011，17，41： | 15 |
| 16 | Effect of six kinds of scale inhibitors on calcium carbonate precipitation in high salinity wastewater at high temperatures显示文摘Precipitation of calcium carbonate(Ca CO3) scale on heat transfer surfaces is a serious and expensive problem widely occurring in numerous industrial processes. In this study, we compared the scale inhibition effect of six kinds of commercial scale inhibitors and screened out the best one(scale inhibitor SQ-1211) to investigate its scale inhibition performance in highly saline conditions at high temperature through static scale inhibition tests. The influences of scale inhibitor dosage, temperature, heating time and p H on the inhibition efficiency of the optimal scale inhibitor were investigated. The morphologies and crystal structures of the precipitates were characterized by Scanning Electron Microscopy and X-ray Diffraction analysis. Results showed that the scale inhibition efficiency of the optimal scale inhibitor decreased with the increase of the reaction temperature. When the concentration of Ca2+was 1600 mg/L, the scale inhibition rate could reach 90.7% at 80°C at p H 8. The optimal scale inhibitor could effectively retard scaling at high temperature. In the presence of the optimal scale inhibitor, the main crystal structure of Ca CO3 changed from calcite to aragonite. | Xiaochen Li Baoyu Gao Qinyan Yue Defang Ma Hongyan Rong Pin Zhao Pengyou Teng | 2015 | Journal of Environmental Sciences2015，27，3： | 15 |
| 17 | Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection显示文摘 | DAI Ning LI De-zhi CHEN Ji-chao CHEN Yu-sheng GENG Rong HU Ying-hui YANG Jing-ping DU Juan HU Cheng-ping ZHANG Wei LI Jia-shu YU Qin WAN Huan-ying MU Lan ZHONG Xiao-ning WEI Li-ping MA Jian-jun WANG Qiu-yue HU Ke TIAN Gui-zhen CAI Shao-xi WANG Rui-qin HE Bei WANG Si-qin WANG Zhan-wei ZHAO Su-rui GAO Zhan-cheng | 2010 | Chinese Medical Journal2010，，18： | 13 |
| 18 | Response to Hepatocarcinoma Hca-F of Mice Immunized with Heat Shock Protein 70 from Elemene Combo Tumor Cell Vaccine显示文摘为了分析有免疫力的反应到老鼠的鼠科的 hepatocarcinoma Hca-F,与热吃惊使免疫(HSP70 ) 蛋白质 70 源于 elemene 组合肿瘤 Hca-F 的房间疫苗(EC-TCV ) , HSP70 被自动数据处理从 EC-TCV 孤立亲密关系层析。老鼠 intraperitoneally 与 HSP70 被使免疫三次,怒气房间被取样。为房间,他们对 Hca-F 的增长和 cytotoxicity 与 MTT 试金被测量,他们的显型与流动 cytometry 被分析。有 HSP70 的使免疫的鼠标的怒气房间比与 EC-TCV 使免疫的鼠标的比正常控制鼠标,而是更少的有势力的对 Hca-F 和增长展出了更多的有势力 cytotoxicity。在三个组之中,在与 HSP70 (35.5%) 使免疫的老鼠的 T 淋巴细胞的百分比在正常老鼠与 6.25% 相比是最高的,并且 28.4% 与 EC-TCV 在老鼠使免疫。从 EC-TCV 导出的 HSP70 的免疫能得到有势力免疫者反应到 Hca-F。HSP70 是对 Hca-F 导致反肿瘤免疫者回答的元素之一。细胞与分子的免疫学。2006; 3 (4 ): 291-295。 | Lianying Guo Guangxia Shi Zhihong Gao Jie Shen Rong Xing Zhenchao Qian | 2006 | Cellular & Molecular Immunology2006，3，4： | 13 |
| 19 | Clinical characteristics of human infection with a novel avian-origin influenza A(H10N8) virus显示文摘 | Zhang Wei Wan Jianguo Qian Kejian Liu Xiaoqing Xiao Zuke Sun Jian Zeng Zhenguo Wang Qi Zhang Jinxiang Jiang Guanghui Nie Cheng Jiang Rong Ding Chengzhi Li Ran Horby Peter Gao Zhancheng | 2014 | Chinese Medical Journal2014，，18： | 12 |
| 20 | Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats显示文摘背景:为心肌的修理的房间移植被早细胞死亡限制。有人的生长激素(hGH ) 的基因治疗被显示了在实验动物支持血管生成和 attenuate apoptosis。这研究被进行在心肌的梗塞以后在心功能恢复和血管生成上探索基于 myoblast 的 hGH 基因治疗的效果,并且比较基于 myoblast 的 hGH 基因治疗和成肌细胞治疗之间的差别。方法:Myoblasts 从几只 SD 老鼠被孤立,有教养,净化,并且有原生质标志 pLghGHSN 和 pLgGFPSN 的 transfected。放射性免疫测定(RIA ) 被用来在这些 myoblasts 检测 hGH 的表示。SD 老鼠经历了左前面的下降冠的动脉的结扎以便建立一个心局部缺血模型。在离开了冠的动脉吸藏以后,经历了结扎的三十幸存老鼠 2 个星期随机被划分成 3 个相等的组:pLghGHSN 组织感染 hGH 基因移植的收到的成肌细胞;pLgGFPSN 组织感染 GFP 基因移植的收到的成肌细胞;控制组织:仅仅收到了有教养的媒介。在注射以后的四个星期幸存老鼠由回响心动描记法经历了心脏的功能的评估。老鼠被打死,室的样品是有 hematoxylin 曙红和第八因子的经历的免疫组织化学。Cryosection 被萤光显微镜检查分析检验绿荧光灯的蛋白质的表示。反向的 transcriptase 聚合酶链反应(RT-PCR ) 被用来检验脉管的内皮生长因素(VEGF ) 的 mRNA 表示, bax 和 Bcl-2。在心肌层的 hGH 表示被西方的污点检验。结果:成肌细胞能成功地被孤立,有教养并且 transfected。在 transfected 成肌细胞的 hGH 的表示与 RIA 被表明。在治疗以后的四个星期,心脏的功能在 pLghGHSN 显著地被改进组和 pLgGFPSN 组。部分弄短(FS ) 并且在 pLghGHSN 组的喷射部分(EF ) 在治疗以后与 pLgGFPSN 组和控制组相比显著地被提高(FS:36.9+/-5.3 对 29.5+/-3.5, 21.8+/-2.9;EF:56.9+/-4.3 对 47.1+/-3.6, 38.4+/-4.8, P<0.05 ) 。左室的结束心脏舒张的尺寸(LVEDD ) 和在 pLghGHSN 组的心 infracted 尺寸在治疗以后与 pLgGFPSN 组和控制组相比显著地被减少(LVEDD:5.9+/-0.3 对 6.8+/-0.2, 8.6+/-0.3;心 infracted 尺寸:(34.5+/-4.2 )% 对(40.0+/-3.9 )% ,(46.1+/-3.8 )% , P<0.05 ) ;格林荧光在 pLgGFPSN 组的 cryosection 被检测。pLgGFPSN 组的毛状的密度比 pLghGHSN 组和控制组(P<0.05 ) 的那些显著地大。在 pLghGHSN 组的 VEGF 和 Bcl-2/bax 的 mRNA 表达式比在 pLgGFPSN 组或控制组(P<0.05 ) 高。在心肌层织物的 hGH 基因的表示能被西方的污点试金在 pLghGHSN 组检测。结论:有 hGH 的心房间 transfected 的移植与 GFP 比房间 transfected 的移植导致了 antiapoptosis 的更大的血管生成和效果。联合 GH 基因转移和房间移植为改进柱子梗塞提供了有效策略室的功能。 | RONG Shu-ling LU Yong-xin LIAO Yu-hua WANG Xiao-lin WANG Yong-jin CHANG Chao WANG Yu-qin LIU Qi-yun GAO Yan-zhang MI Shao-hua | 2008 | Chinese Medical Journal2008，，4： | 12 |
您所在的机构暂未开通此部分的功能，请联系管理员