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| 1 | Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity显示文摘感觉神经原被不同发信号的网络和接受特征区分。因此,感觉神经原类型能被连接基于 transcriptome 的神经原与感觉显型打字定义。这里,我们分类老鼠的 somatosensory 神经原由高范围的单个房间的定序 RNA 的背面的根中心(DRG )(10 950 ±1 218 基因每神经原) 并且神经原基于尺寸的层次聚类。而且,对皮肤的刺激作出回应的单个 DRG 神经原被记录用一在里面 vivo 整个房间的补丁夹钳技术并且由神经原类型分类基因标记。小直径 DRG 神经原被分类进低阀值的 mechanoreceptor 的一种类型和 mechanoheat nociceptors (MHN ) 的五种类型。每 MHN 类型进一步被分成二种子类型。大 DRG 神经原被分成四种类型,包括表示联系 BAI1 的蛋白质 2-like 的 neurexophilin 1 快车 MHN 和机械 nociceptors (MN ) 1 (Baiap2l1 ) 。表示 trafficking 蛋白质粒子建筑群 3-like 和 Baiap2l1 显著的 MN 的 Mechanoreceptors 各被细分进二种子类型。这些结果为编目 somatosensory 神经原和他们的 transcriptome 数据库提供一个新系统。 | Chang-Lin Li Kai-Cheng Li Dan Wu Yan Chen Hao Luo Jing-Rong Zhao Sa-Shuang Wang Ming-Ming Sun Ying-Jin Lu Yan-Qing Zhong Xu-Ye Hu Rui Hou Bei-Bei Zhou Lan Bao Hua-Sheng Xiao Xu Zhang | 2016 | Cell Research2016，26，1： | 21 |
| 2 | Aneuploidy in Early Miscarriage and its Related Factors显示文摘 | Chan-Wei Jia Li Wang Yong-Lian Lan Rui Song Li-Yin Zhou Lan Yu Yang Yang Yu Liang Ying Li Yan-Min Ma Shu-Yu Wang | 2015 | Chinese Medical Journal2015，，20： | 20 |
| 3 | Preparation and electrochemical properties of Sn/C composites显示文摘The metal tin(Sn),as one potential anode material for lithium-ion batteries,rapidly degrades its cyclic performance due to huge volume expansion/contraction during lithium intercalation/de-intercalation process.Amorphous carbon was adopted as conductive and buffer matrix to form Sn/C composites.The products were prepared by hydrothermal reaction and carbothermal reduction using tin tetrachloride and glucose as raw materials.The composites were characterized by X-ray diffraction(XRD),Raman spectroscopy,scanning electron microscopy(SEM),transmission electron microscopy(TEM),cyclic voltammetry(CV) and galvanostatic charge/discharge measurements.The results show that relative smaller metallic tin particles in 1:8 Sn/C composite are formed and distributed more uniformly in the carbon matrix.The lithium intercalation capacity of Sn/C composites reaches 820.4 mAh·g-1,and the capacity retention over 60 cycles remains 54.1%.1:8 Sn/C composite exhibits enhanced rate performance and cyclic stability compared to1:5 and 1:10 samples. | Bei-Ping Wang Rui Lv Dong-Sheng Lan | 2019 | Rare Metals2019，38，10： | 17 |
| 4 | Novel and potent inhibitors targeting DHODH are broad-spectrum antivirals against RNA viruses including newly-emerged coronavirus SARS-CoV-2显示文摘Emerging and re-emerging RNA viruses occasionally cause epidemics and pandemics worldwide,such as the on-going outbreak of the novel coronavirus SARS-CoV-2.Herein,we identified two potent inhibitors of human DHODH,S312 and S416,with favorable drug-likeness and pharmacokinetic profiles,which all showed broad-spectrum antiviral effects against various RNA viruses,including influenza A virus,Zika virus,Ebola virus,and particularly against SARS-CoV-2.Notably,S416 is reported to be the most potent inhibitor so far with an EC5o of 17 nmol/L and an SI value of 10,505.88 in infec-ted cells.Our results are the first to validate that DHODH is an attractive host target through high antiviral efficacy in vivo and low virus replication in DHODH knock-out cells.This work demonstrates that both S312/S416 and old drugs(Leflunomide/Teriflunomide)with dual actions of antiviral and immuno-regulation may have clinical potentials to cure SARS-CoV-2 or other RNA viruses circulating worldwide,no matter such viruses are mutated or not. | Rui Xiong Leike Zhang Shiliang Li Yuan Sun Minyi Ding Yong Wang Yongliang Zhao Yan Wu Weijuan Shang Xiaming Jiang Jiwei Shan Zihao Shen Yi Tong Liuxin Xu Yu Chen Yingle Liu Gang Zou Dimitri Lavillete Zhenjiang Zhao Rui Wang Lili Zhu Gengfu Xiao Ke Lan Honglin Li Ke Xu | 2020 | Protein & Cell2020，11，10： | 14 |
| 5 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
| 6 | Glycyrrhizic acid attenuates CCl_4-induced hepatocyte apoptosis in rats via a p53-mediated pathway显示文摘AIM: To investigate the effect of glycyrrhizic acid (GA) on carbon tetrachloride (CCl4)-induced hepatocyte apo-ptosis in rats via a p53-dependent mitochondrial path-way. METHODS: Forty-five male Sprague-Dawley rats were randomly and equally divided into three groups, the control group, the CCl4 group, and the GA treatment group. To induce liver fibrosis in this model, rats were given a subcutaneous injection of a 40% solution of CCl4 in olive oil at a dose of 0.3 mL/100 g body weight biweekly for 8 wk, while controls received the same isovolumetric dose of olive oil by hypodermic injection, with an initial double-dose injection. In the GA group,rats were also treated with a 40% solution of CCl4 plus 0.2% GA solution in double distilled water by the intraperitoneal injection of 3 mL per rat three times a week from the first week following previously published methods, with modifications. Controls were given the same isovolumetric dose of double distilled water. Liver function parameters, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were de-termined. Pathologic changes in the liver were detected by hematoxylin and eosin staining. Collagen fibers were evaluated by Sirius red staining. Hepatocyte apoptosis was investigated using the terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) assay and the cleaved caspase-3 immunohistochemistry assay. The expression levels of p53 and apoptosis-related proteins were evaluated by immunohistochemistry or Western blotting analysis. RESULTS: After 8 wk of treatment, GA significantly re-duced serum activity of ALT (from 526.7 ± 57.2 to 342 ± 44.8, P<0.05) and AST (from 640 ± 33.7 to 462.8 ± 30.6, P<0.05), attenuated the changes in liver his-topathology and reduced the staging score (from 3.53 ± 0.74 to 3.00 ± 0.76, P<0.05) in CCl4 -treated rats. GA markedly reduced the positive area of Sirius red and the ratio of the hepatic fibrotic region (from 7.87% ± 0.66% to 3.68% ± 0.32%, P<0.05) compared with the CCl4 group. GA also decreased the expression level of cleaved caspase-3 compared to the CCl4 group. TU-NEL assay indicated that GA significantly diminished the number of TUNEL-positive cells compared with the CCl4 group (P<0.05). GA treatment clearly decreased the level of p53 (P<0.05) detected by immunohis-tochemistry and Western blotting analysis. Compared with the CCl4 group, we also found that GA reduced the Bax/Bcl-2 ratio (P<0.05), the expression of cleaved caspase-3 (P<0.05), cleaved caspase-9 (P<0.05), and inhibited cytochrome C and second mitochondria-derived activator of caspases (Smac) release from mito-chondria to cytoplasm, i.e. , GA reduced the expressionlevel of Smac, which inhibited c-IAP1 activity (P<0.05), ultimately inhibiting the activity of caspase-3, according to Western blotting analysis. As a result, GA suppressed activation of the caspase cascades and prevented he-patocyte apoptosis. | Xiao-Ling Guo Bo Liang Xue-Wei Wang Fu-Gang Fan Jing Jin Rui Lan Jing-Hui Yang Xiao-Chun Wang Lei Jin Qin Cao | 2013 | World Journal of Gastroenterology2013，19，24： | 12 |
| 7 | Analysis of inpatient payments of breast cancer patients with different medical insurance coverages in China(mainland) in 2011–2015显示文摘Objective: An understanding of the levels and trends of medical cost is made for breast cancer patients with different medical insurance coverages in China(mainland), in an attempt to offer a clue to further control the costs.Methods: The inpatient payments of 9,716,180 breast cancer patients spent in medical institutions of different types and grades during 2011–2015 were collected from the inpatient medical record home page(IMRHP) dataset.The data were then processed with SAS(Version 9.3; SAS Institute, Cary, NC, USA). Indicators like means,increase(decrease) percentages were used to descriptively analyze the average hospitalization expense of each time(AHEET) and its trends of breast cancer patients with different medical insurance coverages treated in medical institutions of different types and grades.Results:In 2011–2015,the AHEET borne by breast cancer patients in China had been constantly increasing.Specifically,the self-pay inpatients had the largest increase,inpatients covered by Urban Employee Basic Medical Insurance(UEBMI)and Urban Resident Basic Medical Insurance(URBMI)were the next,and those covered by New Rural Cooperative Medical System(NRCMS)had the least increase.Breast cancer inpatient treated in public hospitals had quite greater increase and higher expenditure level than those in private hospitals.The AHEET borne by the inpatients in Grade 3 hospitals had greater increase and higher cost than those in Grade 2 hospitals.Conclusions:The inpatient payments of breast cancer patients will be wisely controlled by reducing the number of self-pay inpatients,taking advantage of restriction mechanism of the medical insurances,and promoting healthy competition between private hospitals and public hospitals.The economic burden imposed on the society by breast cancer can be relieved through further control of inpatient payments of UEBMI-and URBMI-covered breast cancer patients and of Grade 3 hospitals. | Rui Li Liang Zhang Jinxia Yang Yue Cai Wanqing Chen Lan Lan Ming Xue Qun Meng | 2017 | Chinese Journal of Cancer Research2017，29，5： | 9 |
| 8 | Advances in research of the mid-deep South China Sea circulation显示文摘The South China Sea(SCS)is a large marginal sea connecting the Indian and Pacific oceans.Under the factors of monsoons,strait transport,and varied bathymetry,the SCS presents a three-layer structure and strong diapycnal mixing which is far greater than that in the open ocean.Theoretical analysis and observations reveal that internal tides,internal solitary waves,and strong winds are the sources of the strong mixing in the northern SCS.A major consequence of the strong mixing is an active mid-deep circulation system.This system promotes exchange of water between the SCS and adjacent oceans,and also regulates the upper layer of wind-driven circulation,making the 3 dimensional SCS circulation clearly different from that in other tropical and subtropical marginal seas.The mass transport capacity of the mid-deep circulation has a substantial impact on marine sedimentation,the biogeochemical cycle,and other processes in the SCS.This paper summarizes the recent advances in middeep sea circulation dynamics of the SCS,and discusses the opportunities and challenges in this area. | Dongxiao WANG Qiang WANG Shuqun CAI Xiaodong SHANG Shiqiu PENG Yeqiang SHU Jingen XIAO Xiaohui XIE Zhiwei ZHANG Zhiqiang LIU Jian LAN Dake CHEN Huijie XUE Guihua WANG Jianping GAN Xinong XIE Rui ZHANG Hui CHEN Qingxuan YANG | 2019 | Science China Earth Sciences2019，62，12： | 8 |
| 9 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 10 | Simulation of hydrological processes of mountainous watersheds in inland river basins: taking the Heihe Mainstream River as an example显示文摘The hydrological processes of mountainous watersheds in inland river basins are complicated.It is absolutely significant to quantify mountainous runoff for social,economic and ecological purposes.This paper takes the mountainous watershed of the Heihe Mainstream River as a study area to simulate the hydrological processes of mountainous watersheds in inland river basins by using the soil and water assessment tool(SWAT)model.SWAT simulation results show that both the Nash–Sutcliffe efficiency and the determination coefficient values of the calibration period(January 1995 to December 2002)and validation period(January 2002 to December 2009)are higher than 0.90,and the percent bias is controlled within±5%,indicating that the simulation results are satisfactory.According to the SWAT performance,we discussed the yearly and monthly variation trends of the mountainous runoff and the runoff components.The results show that from 1996 to 2009,an indistinctive rising trend was observed for the yearly mountainous runoff,which is mainly recharged by lateral flow,and followed by shallow groundwater runoff and surface runoff.The monthly variation demonstrates that the mountainous runoff decreases slightly from May to July,contrary to other months.The mountainous runoff is mainly recharged by shallow groundwater runoff in January,February,and from October to December,by surface runoff in March and April,and by lateral flow from May to September. | ZhenLiang YIN HongLang XIAO SongBing ZOU Rui ZHU ZhiXiang LU YongChao LAN YongPing SHEN | 2014 | Journal of Arid Land2014，6，1： | 7 |
| 11 | BiCuSeO as state-of-the-art thermoelectric materials for energy conversion:from thin films to bulks显示文摘BiCuSeO-based thermoelectric material has attracted great attention as state-of-the-art thermoelectric materials since it was first reported in 2010. In this review,we update the studies on the BiCuSeO thin films first.Then, we focus on the most recent progress of multiple approaches that enhance the thermoelectric performance including advanced synthesized technologies,notable mechanisms for higher power factor(optimizing carrier concentration, carrier mobility, Seebeck coefficient)and doping effects predicted by calculation. And finally,aiming at further enhancing the performance of these materials and ultimately commercial application, we give a brief discussion on the urgent issues to which should be paid close attention. | Rui Liu Xing Tan Yao-Chun Liu Guang-Kun Ren Jin-Le Lan Zhi-Fang Zhou Ce-Wen Nan Yuan-Hua Lin | 2018 | Rare Metals2018，37，4： | 7 |
| 12 | The molecular characterization of maize B chromosome specific AFLPs显示文摘The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGGGTTT conserved in plant chromosome telomeres. In addition, the sequence of M8-2D shared low homology to clones from maize chromosome 4 centromere as well. M8-2D were localized to B chromosome centrorneric and telomeric regions. | ZHONG XIA QI, Hui ZENG, Xiu LAN LI, CHENG BIN CHEN, WEN QIN SONG, RUI YANG CHEN The College of Life Sciences, Nankai University, Tianjin 300071, China | 2002 | Cell Research2002，12，1： | 7 |
| 13 | Experimental modeling of polymer latex spray coating for producing controlled-release urea显示文摘Spray coating of polymer latex onto fertilizer particles in a fluidized bed for producing controlled-release urea is an environment friendly technology as it does not need any toxic organic solvent. Since the spray coating process in a fluidized bed occurs in the presence of particle collisions, the coating of the particles is random, intermittent and multiple, thus making it difficult to investigate the film formation process. In this paper, an experimental model apparatus was designed and used to investigate the effects of the key factors in the spray coating process. This apparatus reasonably simplified the complex process to avoid particle collisions and randomness in the coating. The intermittent coating in the fluidized bed was modeled by periodic coating and dewatering in the experimental apparatus. A large area film was obtained, and the film permeability was measured. The effects of atomizing gas flow rate, spray rate of latex, solid content of latex and gas temperature on film structure and film permeability were investigated. It was found that water transfer played a dominant role in the spray coating process. | Rui Lan Yonghui Liu Guanda Wang Tingjie Wang Chengyou Kan Yong Jin | 2011 | Particuology2011，9，5： | 6 |
| 14 | Comparative analysis of A,B,C and D genomes in the genus Oryza with C_(0)t-1 DNA of C genome显示文摘Fluorescence in situ hybridization (FISH)was applied to somatic chromosomes preparations of Oryza officinalis Wall. (CC), O. sativa L. (AA)×O. offi-cinalis F1 hybrid (AC), backcross progenies BC1 (AAC and ACC), O. latifolia Desv. (CCDD), O. alta Swallen (CCDD) and O. punctata Kotschy (BBCC) with a labelled probe of C0t-1 DNA from O. officinalis. In O. officinalis, the homologous chromosomes showed similar signal bands probed by C0t-1 DNA and karyotype analysis was conducted based on the band patterns. Using no blocking DNA, the probe identified the chromosomes of C genome clearly, but detected few signals on chromosomes of A genome in the F1 hybrid and two backcross progenies of BC1. It is obvious that the highly and moderately repetitive DNA sequences were considerably different between C and A genomes. The chromosomes of C genome were also discriminated from the chromosomes of D- and B-genome in the tetraploid species O. latifolia, O. alta and O. punctata by C0t-1 DNA-FISH. Compari-son of the fluorescence intensity on the chromo-somes of B, C and D genomes in O. latifolia, O. alta, and O. punctata indicated that the differentiations between C and D genomes are less than that be-tween C and B genomes. The relationship between C and D genomes in O. alta is closer than that of C and D genomes in O. latifolia. This would be one of thecauses for the fact that both the genomes are of the same karyotype (CCDD) but belong to different spe-cies. The above results showed that the C0t-1 DNA had a high specificity of genome and species. In this paper, the origin of allotetraploid in genus Oryza is also discussed. | LAN Weizhen QIN Rui LI Gang HE Guangcun | 2006 | Chinese Science Bulletin2006，51，14： | 6 |
| 15 | Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material显示文摘Stem cell-based therapy has been used to treat ischaemic heart diseases for two decades.However,optimal cell types and transplantation methods remain unclear.This study evaluated the therapeutic effects of human umbilical cord mesenchymal stem cell(hUCMSC)sheet on myocardial infarction(MI).Methods:hUCMSCs expressing luciferase were generated by lentiviral transduction for in vivo bio-luminescent imaging tracking of cells.We applied a temperature-responsive cell culture surface-based method to form the hUCMSC sheet.Cell retention was evaluated using an in vivo bio-luminescent imaging tracking system.Unbiased transcriptional profiling of infarcted hearts and further immunohistochemical assessment of monocyte and macrophage subtypes were used to determine the mechanisms underlying the therapeutic effects of the hUCMSC sheet.Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function,angiogenesis and left ventricular remodelling.Results:When transplanted to the infarcted mouse hearts,hUCMSC sheet significantly improved the retention and survival compared with cell suspension.At the early stage of MI,hUCMSC sheet modulated inflammation by decreasing Mcp1-positive monocytes and CD68-positive macrophages and increasing Cx3cr1-positive non-classical macrophages,preserving the cardiomyocytes from acute injury.Moreover,the extracellular matrix produced by hUCMSC sheet then served as bioactive scaffold for the host cells to graft and generate new epicardial tissue,providing mechanical support and routes for revascularsation.These effects of hUCMSC sheet treatment significantly improved the cardiac function at days 7 and 28 post-MI.Conclusions:hUCMSC sheet formation dramatically improved the biological functions of hUCMSCs,mitigating adverse post-MI remodelling by modulating the inflammatory response and providing bioactive scaffold upon transplantation into the heart.Translational perspective:Due to its excellent availability as well as superior local cellular retention and survival,allogenic transplantation of hUCMSC sheets can more effectively acquire the biological functions of hUCMSCs,such as modulating inflammation and enhancing angiogenesis.Moreover,the hUCMSC sheet method allows the transfer of an intact extracellular matrix without introducing exogenous or synthetic biomaterial,further improving its clinical applicability. | Rui Guo Feng Wan Masatoshi Morimatsu Qing Xu Tian Feng Hang Yang Yichen Gong Shuhong Ma Yun Chang Siyao Zhang Youxu Jiang Heqing Wang Dehua Chang Hongjia Zhang Yunpeng Ling Feng Lan | 2021 | Bioactive Materials2021，6，9： | 6 |
| 16 | MiR-125a-5p Regulates Vitamin D Receptor Expression in a Mouse Model of Experimental Autoimmune Encephalomyelitis显示文摘Multiple sclerosis(MS)is a chronic and incurable autoimmune neurodegenerative disease of the central nervous system.Although the symptoms of MS can be managed by vitamin D3 treatment alone,this condition cannot be completely eradicated.Thus,there might be unknown factors capable of regulating the vitamin D receptor(VDR).Genome-wide analysis showed that miRNAs were associated with VDRs.We sought to determine the role and mechanism of action of miRNA-125a-5p and VDRs in a model of MS,mice with experimental autoimmune encephalomyelitis(EAE),which was induced by myelin oligodendrocyte glycoprotein 35–55 peptides.EAE mice showed decreased mean body weight but increased mean clinical scores compared with vehicle or control mice.And inflammatory infiltration was found in the lumbosacral spinal cord of EAE mice.In addition,VDR expression was significantly lower while the expression of miR-125a-5p was markedly higher in the spinal ventral horn of EAE mice than in vehicle or control mice.Importantly,activation of VDRs by paricalcitol or inhibition of miR-125a-5p by its antagomir markedly decreased the mean clinical scores in EAE mice.Interestingly,VDR and miR-125a-5p were co-localized in the same neurons of the ventral horn.More importantly,inhibition of miR-125a-5p remarkably blocked the decrease of VDRs in EAE mice.These results support a critical role for miR-125a-5p in modulating VDR activity in EAE and suggest potential novel therapeutic interventions. | Han-Chun Long Rui Wu Chun-Feng Liu Fei-Long Xiong Zu Xu Dian He Yi-Fan Zhang Bing Shao Ping-An Zhang Guang-Yin Xu Lan Chu | 2020 | Neuroscience Bulletin2020，36，2： | 6 |
| 17 | EBV-Induced Human CD8^+ NKT Cells Synergise CD4^+ NKT Cells Suppressing EBV-Associated Tumours upon Induction of Thl-Bias显示文摘 | Wei Xiao Li Li Rui Zhou Ruijing Xiao Yujuan Wang Xiang Ji Mengjun Wu Lan Wang Wei Huang Xiaoling Zheng Xinti Tan Lang Chen Tao Xiong Jie Xiong Youxin Jin Jinquan Tan Yuling He | 2009 | Cellular & Molecular Immunology2009，6，5： | 5 |
| 18 | Exposure Effects of Terahertz Waves on Primary Neurons and Neuron-like Cells Under Nonthermal Conditions显示文摘Objective This study aimed to explore the potential effects of terahertz(THz) waves on primary cultured neurons from 4 rat brain regions(hippocampus, cerebral cortex, cerebellum, and brainstem)and 3 kinds of neuron-like cells(MN9 D, PC12, and HT22 cells) under nonthermal conditions.Methods THz waves with an output power of 50(0.16 THz) and 10(0.17 THz) m W with exposure times of 6 and 60 min were used in this study. Analysis of temperature change, neurite growth, cell membrane roughness, micromorphology, neurotransmitters and synaptic-related proteins(SYN and PSD95) was used to evaluate the potential effects.Results Temperature increase caused by the THz wave was negligible. THz waves induced significant neurotransmitter changes in primary hippocampal, cerebellar, and brainstem neurons and in MN9 D and PC12 cells. THz wave downregulated SYN expression in primary hippocampal neurons and downregulated PSD95 expression in primary cortical neurons.Conclusion Different types of cells responded differently after THz wave exposure, and primary hippocampal and cortical neurons and MN9 D cells were relatively sensitive to the THz waves. The biological effects were positively correlated with the exposure time of the THz waves. | TAN Sheng Zhi TAN Peng Cheng LUO Lan Qing CHI Yun Liang YANG Zi Long ZHAO Xue Long ZHAO Li DONG Ji ZHANG Jing YAO Bin Wei XU Xin Ping TIAN Guang CHEN Jian Kui WANG Hui PENG Rui Yun | 2019 | Biomedical and Environmental Sciences2019，32，10： | 5 |
| 19 | Research on design and firing performance of Si-based detonator显示文摘For the chip integration of MEMS(micro-electromechanical system) safety and arming device, a miniature detonator needs to be developed to reduce the weight and volume of explosive train. A Si-based micro-detonator is designed and fabricated, which meets the requirement of MEMS safety and arming device. The firing sensitivity of micro-detonator is tested according to GJB/z377A-94 sensitivity test methods:Langlie. The function time of micro-detonator is measured using wire probe and photoelectric transducer. The result shows the average firing voltage is 6.4 V when the discharge capacitance of firing electro-circuit is 33 mF. And the average function time is 5.48 ms. The firing energy actually utilized by Si-based micro-detonator is explored. | Rui-zhen XIE Xiao-ming REN Lan LIU Yan XUE Dong-xiao FU Rui ZHANG | 2014 | Defence Technology(防务技术)2014，10，1： | 5 |
| 20 | Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses显示文摘Human skeletal stem cells(SSCs)have been discovered in fetal and adult long bones.However,the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive.Here we map the transcriptional landscape of human limb buds and embryonic long bones at single-cell resolution to address this fundamental question.We found remarkable heterogeneity within human limb bud mesenchyme and epithelium,and aligned them along the proximal-distal and anterior-posterior axes using known marker genes.Osteo-chondrogenic progenitors first appeared in the core limb bud mesenchyme,which give rise to multiple populations of stem/progenitor cells in embryonic long bones undergoing endochondral ossification.Importantly,a perichondrial embryonic skeletal stem/progenitor cell(eSSPC)subset was identified,which could self-renew and generate the osteochondral lineage cells,but not adipocytes or hematopoietic stroma.eSSPCs are marked by the adhesion molecule CADM1 and highly enriched with FOXP1/2 transcriptional network.Interestingly,neural crest-derived cells with similar phenotypic markers and transcriptional networks were also found in the sagittal suture of human embryonic calvaria.Taken together,this study revealed the cellular heterogeneity and lineage hierarchy during human embryonic skeletogenesis,and identified distinct skeletal stem/progenitor cells that orchestrate endochondral and intramembranous ossification. | Jian He Jing Yan Jianfang Wang Liangyu Zhao Qian Xin Yang Zeng Yuxi Sun Han Zhang Zhijie Bai Zongcheng Li Yanli Ni Yandong Gong Yunqiao Li Han He Zhilei Bian Yu Lan Chunyu Ma Lihong Bian Heng Zhu Bing Liu Rui Yue | 2021 | Cell Research2021，31，7： | 5 |
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