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| 1 | Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha显示文摘The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources. | Zhangxiong Liu Jun Li Xuhong Fan Nang Myint Phyu Sin Htwe Shuming Wang Wen Huang Jiyu Yang Lili Xing Lijun Chen Yinghui Li Rongxia Guan Ruzhen Chang Dechun Wang Lijuan Qiu | 2017 | The Crop Journal2017，5，4： | 10 |
| 2 | Sheep manure application increases soil exchangeable base cations in a semi-arid steppe of Inner Mongolia显示文摘The long-term productivity of a soil is greatly influenced by cation exchange capacity(CEC).Moreover,interactions between dominant base cations and other nutrients are important for the health and stability of grassland ecosystems.Soil exchangeable base cations and cation ratios were examined in a 11-year experiment with sheep manure application rates 0–1,500 g/(m2?a) in a semi-arid steppe in Inner Mongolia of China,aiming to clarify the relationships of base cations with soil p H,buffer capacity and fertility.Results showed that CEC and contents of exchangeable calcium(Ca2+),magnesium(Mg2+),potassium(K+) and sodium(Na+) were significantly increased,and Ca2+ saturation tended to decrease,while K+ saturation tended to increase with the increases of sheep manure application rates.The Ca2+/Mg2+ and Ca2+/K+ ratios decreased,while Mg2+,K+ and Na+ saturations increased with increasing manure application rates.Both base cations and CEC were significantly and positively correlated with soil organic carbon(SOC) and soil p H.The increases of SOC and soil p H would be the dominant factors that contribute to the increase of cations in soil.On a comparison with the initial soil p H before the experiment,we deduced that sheep manure application could partly buffer soil p H decrease potentially induced by atmospheric deposition of nitrogen and sulfur.Our results indicate that sheep manure application is beneficial to the maintenance of base cations and the buffering of soil acidification,and therefore can improve soil fertility in the semi-arid steppes of northeastern China. | YuGe ZHANG Shan YANG MingMing FU JiangPing CAI YongYong ZHANG RuZhen WANG ZhuWen XU YongFei BAI Yong JIANG | 2015 | Journal of Arid Land2015，7，3： | 7 |
| 3 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 4 | Mapping and validation of a dominant salt tolerance gene in the cultivated soybean(Glycine max) variety Tiefeng 8显示文摘Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng 8.The objective of the present study was to genetically map the salt-tolerance gene in an F2:3population and a recombinant inbred line(RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8(tolerant) and 85-140(sensitive). The F2:3families and RILs were treated with 200 mmol L-1Na Cl to evaluate salt tolerance. The F2:3population showed 1(42 tolerant): 2(132 segregating): 1(65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region(SCAR) marker from a previously identified random amplified polymorphic DNA(RAPD)marker and four insertion/deletion polymorphism(In Del) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_3_1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean. | Rongxia Guan Jiangang Chen Jinghan Jiang Guangyu Liu Ying Liu Lei Tian Lili Yu Ruzhen Chang Li-juan Qiu | 2014 | The Crop Journal2014，2，6： | 5 |
| 5 | Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis显示文摘Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress. | ZHOU GuoAn GUAN RongXia LI YingHui CHANG RuZhen QIU LiJuan | 2009 | Chinese Science Bulletin2009，54，19： | 4 |
| 6 | Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers显示文摘Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25. | QIN Jun CHEN Weiyuan GUAN Rongxia JIANG Chengxi LI Yinghui FU Yashu LIU Zhangxiong ZHANG Mengchen CHANG Ruzhen QIU Lijuan | 2006 | Chinese Science Bulletin2006，51，9： | 4 |
| 7 | A review of the techniques for the mold manufacturing of micro/nanostructures for precision glass molding显示文摘Micro/nanostructured components play an important role in micro-optics and optical engineering,tribology and surface engineering,and biological and biomedical engineering,among other fields.Precision glass molding technology is the most efficient method of manufacturing micro/nanostructured glass components,the premise of which is meld manufacturing with complementary micro/nanostructures.Numerous mold manufacturing methods have been developed to fabricate extremely small and high-quality micro/nanostructures to satisfy the demands of functional micro/nanostructured glass components for various applications.Moreover,the service performance of the mold should also be carefully considered.This paper reviews a variety of technologies for manufacturing micro/nanostructured molds.The authors begin with an introduction of the extreme requirements of mold materials.The following section provides a detailed survey of the existing micro/nanostructured mold manufacturing techniques and their corresponding mold materials,including nonmechanical and mechanical methods.This paper concludes with a detailed discussion of the authors recent research on nickel-phosphorus(Ni-P)mold manufacturing and its service performance. | Tianfeng Zhou Yupeng He Tianxing Wang Zhanchen Zhu Ruzhen Xu Qian Yu Bin Zhao Wenxiang Zhao Peng Liu Xibin Wang | 2021 | International Journal of Extreme Manufacturing2021，3，4： | 3 |
| 8 | Enhanced carbon acquisition and use efficiency alleviate microbial carbon relative to nitrogen limitation under soil acidification显示文摘Background:Soil microbial communities cope with an imbalanced supply of resources by adjusting their element acquisition and utilization strategies.Although soil pH has long been considered an essential driver of microbial growth and community composition,little is known about how soil acidification affects microbial acquisition and utilization of carbon(C)and nitrogen(N).To close the knowledge gap,we simulated soil acidification and created a pH gradient by adding eight levels of elemental sulfur(S)to the soil in a meadow steppe.Results:We found that S-induced soil acidification strongly enhanced the ratio of fungi to bacteria(F:B)and microbial biomass C to N(MBC:MBN)and subsequently decreased the C:N imbalance between microbial biomass and their resources.The linear decrease in the C:N imbalance with decreasing soil pH implied a conversion from N limitation to C limitation.To cope with enhanced C versus N limitation,soil microbial communities regulated the relative production of enzymes by increasing the ratio ofβ-glucosidase(BG,C-acquiring enzyme)to leucine aminopeptidase(LAP,N-acquiring enzyme),even though both enzymatic activities decreased with S addition.Structural equation modeling(SEM)suggested that higher C limitation and C:N-acquiring enzyme stimulated microbial carbon-use efficiency(CUE),which counteracted the negative effect of metal stress(i.e.,aluminum and manganese)under soil acidification.Conclusions:Overall,these results highlight the importance of stoichiometric controls in microbial adaption to soil acidification,which may help predict soil microbial responses to future acid deposition. | Tianpeng Li Ruzhen Wang Jiangping Cai Yani Meng Zhirui Wang Xue Feng Heyong Liu Ronald F.Turco Yong Jiang | 2021 | Ecological Processes2021，10，1： | 2 |
| 9 | Implementation of Dynamic System Control Circuit Board Test System of Pure Electric Vehicles显示文摘The dynamic system control circuit board(DSCCB)is one of the most important components for dynamic system of pure electric vehicles. The current detection of the DSCCB is done manually, which is not only inefficient in the detection but also difficult to guarantee the data accuracy. In order to improve the detection efficiency and accuracy, a new testing system is designed by Labview. The total test time can be further reduced by about 75% compared with the results of the manual detection. In this paper, the three-parameter sine wave curve-fit algorithm theory is applied to the phase delay detection of the current sensor sampling circuit on the DSCCB. This method solves the problem of big error in the phase delay detection. | BI Wei, WANG Jianming, DOU Ruzhen, GAO Hua (Department of Information and Communication, Tianjin Polytechnic University, Tianjin 300160, CHN) | 2010 | Semiconductor Photonics and Technology2010，16，4： | 2 |
| 10 | The study of high efficient AgI pyrotechnics and their nucleating properties显示文摘 | Feng Daxiong Chen Ruzhen Jiang Gengwang | 1994 | Acta Meteo-rological Sinica1994，8，3： | 1 |
| 11 | Optimization of totalflavonoid compound extraction from gynuramedica leaf u- sing responsesurfaeemcthodology and chemical composition analy sis显示文摘 | LIU WEI YU YANYING YANG RUZHEN | 2010 | Int J MolSci2010，11，11： | 1 |
| 12 | Discrete particle simulation of particulate systems:theoretical developments显示文摘 | ZHU Haiping ZHOU Zongyan YANG Ruzhen | 2007 | Chemical Engineering Science2007，62，13： | 1 |
| 13 | Cloning and sequence diversity analysis of GmHs1 pro-1 in Chinese domesticated and wild soybeans显示文摘 | Cuiping Yuan Guoan Zhou Yinghui Li Kejing Wang Zhi Wang Xianghua Li Ruzhen Chang Lijuan Qiu | 2008 | Molecular Breeding2008，，4： | 1 |
| 14 | Preparation of Niobium Metal by the Aluminothermic Reduction of Its Pentoxide 显示文摘 | Zhong Haiyun Wang Ruzhen Feng Shuifu | 1987 | Zhongnan Kuangye Xueyuan Xuebao1987，18，4： | 1 |
| 15 | Genome-wide signatures of the geographic expansion and breeding of soybean显示文摘Soybean is a leguminous crop that provides oil and protein. Exploring the genomic signatures of soybean evolution is crucial for breeding varieties with improved adaptability to environmental extremes. We analyzed the genome sequences of 2,214 soybeans and proposed a soybean evolutionary route, i.e., the expansion of annual wild soybean(Glycine soja Sieb. & Zucc.) from southern China and its domestication in central China, followed by the expansion and local breeding selection of its landraces(G. max(L.) Merr.). We observed that the genetic introgression in soybean landraces was mostly derived from sympatric rather than allopatric wild populations during the geographic expansion. Soybean expansion and breeding were accompanied by the positive selection of flowering time genes, including GmSPA3c. Our study sheds light on the evolutionary history of soybean and provides valuable genetic resources for its future breeding. | Ying-Hui Li Chao Qin Li Wang Chengzhi Jiao Huilong Hong Yu Tian Yanfei Li Guangnan Xing Jun Wang Yongzhe Gu Xingpeng Gao Delin Li Hongyu Li Zhangxiong Liu Xin Jing Beibei Feng Tao Zhao Rongxia Guan Yong Guo Jun Liu Zhe Yan Lijuan Zhang Tianli Ge Xiangkong Li Xiaobo Wang Hongmei Qiu Wanhai Zhang Xiaoyan Luan Yingpeng Han Dezhi Han Ruzhen Chang Yalong Guo Jochen C.Reif Scott A.Jackson Bin Liu Shilin Tian Li-juan Qiu | 2023 | Science China(Life Sciences)2023，66，2： | 1 |
| 16 | Research on Change of Rhizosphere Soil Properties of Chinese fir Plantation显示文摘This article emphatically reviews the difference of soil biological activities, biochemical activities and soil chemical properties between the rhizosphere and non-rhizosphere soil of first rotation of Chinese fir (Cunninghamia lanceolata (Lamb) Hook) plantation. It also reviews their dynamic patterns during Chinese fir plantation development. The results show that the contents of organic and inorganic nutrients in the rhizosphere soil of young, half-mature and near-mature Chinese fir of first-rotation ... | YANG Chengdong JIAO Ruzhen SUN QiwuResearch Institute of Forestry. Chinese Academy of Forestry. Beijing 100091. China | 2002 | Chinese Forestry Science and Technology2002，1，4： | 1 |
| 17 | Optimization of totalflavonoid compound extraction from Gynura medica leaf usingresponse surface methodology and chemical composition analysis显示文摘 | LIU Wei YU Yanying YANG Ruzhen | 2010 | International Journal of Molecular Sciences2010，11，11： | 1 |
| 18 | Antioxidant Activity of Pine Needle Chlorophyllins, Food Technol 显示文摘 | Ruzhen Zhan Jian Wu | 2014 | Biotechnol2014，52，4： | 1 |
| 19 | Walnut shell-based activa?ted carbon with excellent copper ( II ) adsorption and lower chro?mium CVI) removal prepared by acid-base modification显示文摘 | Xie Ruzhen Wang Hui Chen Yao | 2013 | Environ Prog Sustainable Energy2013，32，3： | 1 |
| 20 | Responses and sensitivity of N, P and mobile carbohydrates of dominant species to increased water, N and P availability in semi- arid grasslands in northern China显示文摘Aims We aimed to improve the understanding of the carbon and nutri-ent physiological responses and adaptation of semi-arid grassland plants to environmental changes.Methods We investigated plant leaf non-structural carbohydrate(NSC=solu-ble sugars+starch),nitrogen(N)and phosphorus(P)levels in an Inner Mongolian semi-arid grassland community treated with water,N and P additions for 8 years.Two dominant grasses(Agropyron cris-tatum(L.)Gaertn.,Stipa krylovii Roshev.)and two forbs(Artemisia frigida Willd.,Potentilla bifurca L.)were analyzed.Important Findings Water addition decreased plant leaf N and P concentrations,whereas N and P addition increased them,indicating that the semi-arid grassland studied suffers from a shortage of N and P sup-ply.Both N and P addition decreased the levels of soluble sugars,starch and thus also NSC in plant leaves,which may be attributed to(i)increased carbohydrate consumption associated with a higher growth rate,and(ii)a dilution effect of greater plant size under N and P addition.Water addition tended to increase the leaf NSC levels both in the grasses(+9.2%)and forbs(+0.6%only),which may be a result of increased photosynthesis of plants with increased water availability.Under conditions of ambient and increased water supply in the present study,N addition resulted in an N/P ratio of>16 in the grasses but a significantly lower N/P ratio of<11 in the forb species.This finding implies that growth of the two grass spe-cies will be limited mainly by P availability but the forbs will still be mainly limited by N supply if N deposition,alone or in combination with summer precipitation,continues to increase as predicted in Inner Mongolia. | Xue Wang Zhuwen Xu Caifeng Yan Wentao Luo Ruzhen Wang Xingguo Han Yong Jiang Mai-He Li | 2017 | Journal of Plant Ecology2017，10，3： | 1 |
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