|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Nonhuman primate models of focal cerebral ischemia显示文摘Rodents have been widely used in the production of cerebral ischemia models. However, successful therapies have been proven on experimental rodent stroke model, and they have often failed to be effective when tested clinically. Therefore, nonhuman primates were recommended as the ideal alternatives, owing to their similarities with the human cerebrovascular system, brain metabolism, grey to white matter ratio and even their rich behavioral repertoire. The present review is a thorough summary of ten methods that establish nonhuman primate models of focal cerebral ischemia; electrocoagulation, endothelin-1-induced occlusion, microvascular clip occlusion, autologous blood clot embolization, balloon inflation, microcatheter embolization, coil embolization, surgical suture embolization, suture, and photochemical induction methods. This review addresses the advantages and disadvantages of each method, as well as precautions for each model, compared nonhuman primates with rodents, different species of nonhuman primates and different modeling methods. Finally it discusses various factors that need to be considered when modelling and the method of evaluation after modelling. These are critical for understanding their respective strengths and weaknesses and underlie the selection of the optimum model. | Jingjing Fan Yi Li Xinyu Fu Lijuan Li Xiaoting Hao Shasha Li | 2017 | Neural Regeneration Research2017，12，2： | 14 |
| 2 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 3 | High-throughput sequencing exclusively identified a novel Torque teno virus genotype in serum of a patient with fatal fever显示文摘Torque teno virus(TTV) has been found to be prevalent world-wide in healthy populations and in patients with various diseases, but its etiological role has not yet been determined. Using high-throughput unbiased sequencing to screen for viruses in the serum of a patient with persistent high fever who died of suspected viral infection and prolonged weakness, we identified the complete genome sequence of a TTV(isolate Hebei-1). The genome of TTV-Hebei-1 is 3649 bp in length, encoding four putative open reading frames, and it has a G+C content of 49%. Genomic comparison and a BLASTN search revealed that the assembled genome of TTV-Hebei-1 represented a novel isolate, with a genome sequence that was highly heterologous to the sequences of other reported TTV strains. A phylogenetic tree constructed using the complete genome sequence showed that TTV-Hebei-1 and an uncharacterized Taiwan Residents strain, TW53A37, constitute a new TTV genotype. The patient was strongly suspected of carrying a viral infection and died eventually without any other possible causes being apparent. No virus other than the novel TTV was identified in his serum sample. Although a direct causal link between the novel TTV genotype infection and the patient's disease could not be confirmed, the findings suggest that surveillance of this novel TTV genotype is necessary and that its role in disease deserves to be explored. | Zhiqiang Mi Xin Yuan Guangqian Pei Wei Wang Xiaoping An Zhiyi Zhang Yong Huang Fan Peng Shasha Li Changqing Bai Yigang Tong | 2014 | Virologica Sinica2014，29，2： | 4 |
| 4 | Association between thyroid hormone levels and frailty in the community-dwelling oldest-old: a cross-sectional study显示文摘Background:Changes in thyroid hormone levels are commonly recognized characters among the elderly,which were reported to potentially influence incident frailty.Therefore,we examined the cross-sectional associations of thyroid hormones(THs)with frailty as well as the five components characterizing frailty(fatigue,resistance,ambulation,number ofillnesses,and loss of weight)among the oldest-old.Methods:Four hundred and eighty-seven community-dwelling oldest-old from a local community in Haidian District,Beijing,participated in our recruitment campaign between April 2019 and May 2020.The primary outcomes were a definitive diagnosis of frailty according to the FRAIL scale(Fatigue,Resistance,Ambulation,Illnesses,Loss of weight)and a positive score for each frailty subdomain.Demographic information(age,sex,marital status,and educational status),comorbidities,and details on the participants lifestyles were recorded.Serum THs including free triodothyronin(fT3),triiodothyronine(T3),free thyroxine(fT4),and thyroxine(T4)and thyroid stimulating hormone(TSH)levels were also measured at the beginning of our study.Logistic regressions were conducted to screen for potential risk factors for frailty and its subdomains.Results:Among the total 487 subjects at enrollment,60(12.23%)of them were diagnosed with subclinical hypothyroidism and 110(22.59%)of the total population scored positive for frailty.Logistic regression analyses adjusted for all potential confounders,showed that frailty was significantly associated with the serum TSH concentration(odds ratio[OR]:1.06),fT3 concentration(OR:0.54),and subclinical hypothyroidism score(OR:2.18).The association between fT4 and frailty was absent in our observational study.The fT3/fT4 ratio characterizing peripheral hormone conversion was also tested to be correlated with frailty.Conclusion:Subclinical hypothyroidism,higher TSH level,lower fT3 level,and decreased fT3/fT4 ratio were all associated with frailty assessed by the FRAIL scale among the community-dwelling oldest-old,suggesting a relevant role of thyroid function in aging.Future longitudinal studies are warranted to determine the casual relationship between thyroid dysfunction and frailty in the oldest-old. | Bo Guan Jiakun Luo Xin Huang Fan Tian Shasha Sun Yufei Ma Yan Yu Ruihan Liu Jian Cao Li Fan | 2022 | Chinese Medical Journal2022，135，16： | 2 |
| 5 | Activated coke impregnated with cerium chloride used for elemental mercury removl from simulated flue gas 显示文摘 | Tao Shasha Li Caiting Fan Xiaopeng | 2012 | Chemical Engineering Journal2012，210，： | 1 |
| 6 | Enhanced CO2 adsorption capacity and stability using CaO‐based adsorbents treated by hydration显示文摘 | Shengping Wang Hui Shen Shasha Fan Yujun Zhao Xinbin Ma Jinlong Gong | 2013 | AIChE J2013，，10： | 1 |
| 7 | Novel CeO2 yolk-shell structures loaded with tiny Au nanoparti- cles for superior catalytic reduction of p-nitrophenol 显示文摘 | Fan Congmin Zhang Linfei Wang Shasha | 2012 | Nanoscale2012，4，21： | 1 |
| 8 | Somatic Embryogenesis in Lily Bulb Scale Cultures显示文摘Somatic embryogenesis from lily bulb scales has not been studied in details,although tissue culture methods have been applied to the propagation for decades.The effects of different kinds and concentration of auxins for oriental lily somatic embryogenesis were investigated(Lilium hybrida var.Sorbonne).2,4-dichlorophenoxyacetic acid(2,4-D),thidiazuron(TDZ)and α-naphthaleneacetic acid(NAA)media with benzyladenine(6-BA)and lactalbumin hydrolysate(LH)were used for embryogenic callus in the darkness.The best response on embryogenic callus formation was obtained on MS media supplemented 2,4-D 2.0 mg?L-1, 6-BA 0.5 mg·L-1 and LH 300 mg·L-1.Transfer embryogenic callus to the media with TDZ,6-BA,kinetin(KT)supplemented 2,4-D. The highest number of somatic embryos has been produced on medium with 0.5 mg·L-1 2,4-D and 0.3 mg?L-1 KT.Germinated embryos with shoot axes were changed to MS media with 6-BA 0.5 mg?L-1.The results suggest that in vitro culture of somatic embryogenesis from lily bulb scales can be used for plant regeneration. | WANG Shasha WANG Jingang FAN Jinping CHE Daidi | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，4： | 1 |
| 9 | FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect显示文摘African swine fever(ASF)is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar,which is caused by the African swine fever virus(ASFV).ASF has caused huge economic losses to the pig industry and seriously threatens global food security and livestock health.To date,there is no safe and effective commercial vaccine against ASF.Unveiling the underlying mechanisms of ASFV-host interplay is critical for developing effective vaccines and drugs against ASFV.In the present study,RNA-sequencing,RT-qPCR and Western blotting analysis revealed that the transcriptional and protein levels of the host factor FoxJ1 were significantly down-regulated in primary porcine alveolar macrophages(PAMs)infected by ASFV.RT-qPCR analysis showed that overexpression of FoxJ1 upregulated the transcription of type I interferon and interferon stimulating genes(ISGs)induced by poly(dA:dT).FoxJ1 revealed a function to positively regulate innate immune response,therefore,suppressing the replication of ASFV.In addition,Western blotting analysis indicated that FoxJ1 degraded ASFV MGF505-2R and E165R proteins through autophagy pathway.Meanwhile,RT-qPCR and Western blotting analysis showed that ASFV S273R inhibited the expression of FoxJ1.Altogether,we determined that FoxJ1 plays an antiviral role against ASFV replication,and ASFV protein impairs FoxJ1-mediated antiviral effect by degradation of FoxJ1.Our findings provide new insights into the antiviral function of FoxJ1,which might help design antiviral drugs or vaccines against ASFV infection. | Caina Ma Shasha Li Fan Yang Weijun Cao Huisheng Liu Tao Feng Keshan Zhang Zixiang Zhu Xiangtao Liu Yonghao Hu Haixue Zheng | 2022 | Virologica Sinica2022，37，3： | 1 |
| 10 | S2-Net:Self-Supervision Guided Feature Representation Learning for Cross-Modality Images显示文摘Dear Editor,This letter focuses on combining the respective advantages of cross-modality images which can compensate for the lack of information in the single modality.Meanwhile,due to the great appearance differences between cross-modality image pairs,it often fails to make the feature representations of correspondences as close as possible. | Shasha Mei Yong Ma Xiaoguang Mei Jun Huang Fan Fan | 2022 | IEEE/CAA Journal of Automatica Sinica2022，9，10： | 0 |
| 11 | Co-thermal in-situ reduction of inorganic carbonates to reduce carbon-dioxide emission显示文摘Thermal decomposition of inorganic metal carbonates is the main path to prepare metal oxides;nonetheless,it is always accompanied by the emission of large amounts of CO_(2) as one of the gas products.This study reports a concept of co-thermal insitu reduction of inorganic carbonates by using the energy released by carbonate decomposition under pure hydrogen atmosphere,which reduces the decarboxylation temperature and significantly inhibits the CO_(2) emissions.A combination of hydrogen–deuterium exchange,isotope experiment,and density functional theory calculations demonstrates that the CO results from the selective cleavage of Ca–O bonds at the surface of CaCO_(3) via the direct hydrogenation mechanism at relatively low temperature.However,it undergoes the reverse water–gas shift reaction path at high temperature,i.e.,CO being produced by the reduction of CO_(2) released by the decomposition of carbonates.This study sheds light on the potential of green hydrogen technology for inorganic carbonate valorization toward high value-added products,which can facilitate the large-scale industrial applications. | Zhen Xue Jingyi Guo Shasha Wu Wenfu Xie Yujing Fu Xiaojie Zhao Kui Fan Ming Xu Hong Yan Mingfei Shao Xue Duan | 2023 | Science China Chemistry2023，66，4： | 0 |
| 12 | Legume-specific SnRK1 promotes malate supply to bacteroids for symbiotic nitrogen fixation显示文摘Nodulation is an energy-expensive behavior driven by legumes by providing carbon sources to bacteroids and obtaining nitrogen sources in return.The energy sensor sucrose nonfermenting 1-related protein kinase 1(SnRK1)is the hub of energy regulation in eukaryotes.However,the molecular mechanism by which SnRK1 coordinates the allocation of energy and substances during symbiotic nitrogen fixation(SNF)remains unknown.In this study,we identified the novel legume-specific SnRK1α4,a member of the SnRK1 family that positively regulates SNF.Phenotypic analysis showed that nodule size and nitrogenase activity increased in SnRK1α4-overexpressing plants and decreased significantly in snrk1α4 mutants.We demonstrated that a key upstream kinase involved in nodulation,Does Not Make Infection 2(DMI2),can phosphorylate SnRK1α4 at Thr175 to cause its activation.Further evidence clarified that SnRK1α4 phosphorylates the malate dehydrogenases MDH1/2 to promote malate production in the cytoplasm,supplying carbon sources to bacteroids.Therefore,our findings reveal an essential role of the DMI2–SnRK1α4–MDH pathway in supplying carbon sources to bacteroids for SNF and provide a new module for constructing cereal crops with SNF. | Da Guo Peng Liu Qianwen Liu Lihua Zheng Sikai Liu Chen Shen Li Liu Shasha Fan Nan Li Jiangli Dong Tao Wang | 2023 | Molecular Plant2023，16，9： | 0 |
| 13 | Systemic and single cell level responses to 1 nm size biomaterials demonstrate distinct biological effects revealed by multi-omics atlas显示文摘Although ultra-small nanoclusters(USNCs,<2 nm)have immense application capabilities in biomedicine,the investigation on body-wide organ responses towards USNCs is scant.Here,applying a novel strategy of single-cell mass cytometry combined with Nano Genome Atlas of multi-tissues,we systematically evaluate the interactions between the host and calcium phosphate(CaP)USNCs at the organism level.Combining single-cell mass cytometry,and magnetic luminex assay results,we identify dynamic immune responses to CaP USNCs at the single cell resolution.The innate immune is initially activated and followed by adaptive immune activation,as evidenced by dynamic immune cells proportions.Furthermore,using Nano Genome Atlas of multi-tissues,we uncover CaP USNCs induce stronger activation of the immune responses in the cartilage and subchondral bone among the five local tissues while promote metabolic activities in the liver and kidney.Moreover,based on the immunological response profiles,histological evaluation of major organs and local tissue,and a body-wide transcriptomics,we demonstrate that CaP USNCs are not more hazardous than the Food and Drug Administration-approved CaP nanoparticles after 14 days of injection.Our findings provide valuable information on the future clinical applications of USNCs and introduce an innovative strategy to decipher the whole body response to implants. | Tao Zhang Tingyun Lei Ruojin Yan Bo Zhou Chunmei Fan Yanyan Zhao Shasha Yao Haihua Pan Yangwu Chen Bingbing Wu Yuwei Yang Lijuan Hu Shen Gu Xiaoyi Chen Fangyuan Bao Yu Li Hanqi Xie Ruikang Tang Xiao Chen Zi Yin | 2022 | Bioactive Materials2022，7，12： | 0 |
| 14 | Rubicon deficiency exacerbates fasting-induced hepatic steatosis显示文摘Objective: Rubicon is an inhibitory interacting protein of the autophagy-related protein Uvrag. We previously showed thatRubicon deficiency promotes autophagic fluxin vivo and that autophagy can degrade lipid droplets. This study aimed to investigate the effects of Rubicon deficiency on fasting-induced hepatic steatosis.Methods: Two-month-old wild-type (WT) andRubicon-deficient mice were subjected to feeding or fasting for 24 hours to induce hepatic steatosis. The distribution of liver lipid droplets was revealed by oil red O staining. Hepatic and plasma triglyceride, non-esterified fatty acid (NEFA), and cholesterol levels were detected using commercially available kits. Real-time reverse transcriptasepolymerase chain reaction was performed to analyze the mRNA expression of genes related to lipid metabolism in the liver. Western blot was conducted to assess autophagy-related protein levels in the liver. The animal experiments were approved by the Institutional Animal Care and Use Committee at Shanghai Jiao Tong University, China.Results: We showed that under fasting conditions,Rubicon-deficient mice had more lipid droplets in the liver than WT controls. Consistent with these results, the hepatic triglyceride, NEFA, and cholesterol levels in fastedRubicon-deficient mice were significantly higher than those of fasted WT controls. The levels ofSREBP-1, a key regulator of lipid synthesis, were significantly lower in livers from fasted WT mice than those of fed WT mice. However, the decrease inSREBP-1 in fasted mice was attenuated byRubicon deficiency. Western blot analysis demonstrated that the fasting-induced increase in autophagic flux was amplified byRubicon deficiency. Finally, we showed thatRubicon deficiency in mice led to elevated plasma triglyceride and NEFA acid levels under fasting conditions.Conclusion: Rubicon deficiency exacerbates fasting-induced hepatic steatosis in mice. | Fan Dong Xiao-Wen Hu Shasha Zhang Fan He Amber Naz Lin He Hongxin Zhu | 2022 | Journal of Bio-X Research2022，5，1： | 0 |
| 15 | A nanoscale observation to explain the discrepancy of electron exchange capacities between biochar containing comparable surface redox-active moieties显示文摘Biochar,possessing electron exchange capacities(EEC),is generally involved in environmental redox reactions due to the presence of redox-active moieties(RAMs).The phenomenon that chars containing comparable RAMs possess differential EEC revealed that the accessibility of RAMs is important to the redox properties.However,many studies have focused on the type of RAMs,whereas the distribution has been insufficiently investigated.Herein,we achieved nanoscale observation of electroactive moieties on the surface of six chars using a conductive atomic force microscope.For the two specific kinds of chars with submicron particles and opposite current distributions,the submicron particles took up only 1-4‰wt of biochar accounting for approximately 30-50%of electron-donating capacity(EDC),and electron-accepting capacity(EAC)became 87%and 1.40 times as before after removing submicron particles,respectively.Meanwhile,the combined impact of RAMs and surface topography(that uneven distribution of RAMs resulted in outstanding EEC by enhancing accessibility)was clarified.Furthermore,direct evidence of the link between char structure and EEC(that condensed aromatic structures were indispensable to EAC while both heteroatoms and amorphous aromatics contributed to EDC)was established.These findings can aid in understanding the functions of biochar in biotic and abiotic redox processes. | Shasha Li Liming Shao Hua Zhang Xuemin Lu Fan Lü Pinjing He | 2022 | Biochar2022，4，1： | 0 |
| 16 | Power Quality Dectection and Classification in Active Distribution Networks Based on Improved Empiricial Wavelet Transform and Disperson Entropy显示文摘In this paper,a model including wind power generation,photovoltaic power generation and electric vehicle for high permeability active distribution network(ADN)is established.The power quality(PQ)disturbance signals in the high permeability are extracted,and the characteristics of disturbance signals are analyzed in the situation of grid connection,interruption and islanding.The multi-scale fluctuation dispersion entropy(MFDE)initialized by the improved empirical wavelet transform(IEWT)is utilized to detect and classify the disturbance signals in the high permeability ADN.First,the eigenvectors of the disturbance signals are obtained by using the multi-scale fluctuation dispersion entropy initiated by the IEWT,and then the reduced eigenvectors are put into the support vector machine to classify the PQ disturbances caused by the access of the different distributed generators accessed.The classification results are compared with that in the traditional methods and other similar ways;the effectiveness of the IEWT-MFDE system is verified. | Yanchun Xu Shirong Fan Shasha Xie Mi Lu | 2022 | CSEE Journal of Power and Energy Systems2022，8，6： | 0 |
| 17 | Porcine epidemic diarrhea virus nsp14 inhibits NF-κB pathway activation by targeting the IKK complex and p65显示文摘Coronaviruses(CoVs)are a group of related enveloped RNA viruses that have severe consequences in a wide variety of animals by causing respiratory,enteric or systemic diseases.Porcine epidemic diarrhea virus(PEDV)is an economically important CoV distributed worldwide that causes diarrhea in pigs.nsp14 is a nonstructural protein of PEDV that is involved in regulation of innate immunity and viral replication.However,the function and mechanism by which nsp14 modulates and manipulates host immune responses remain largely unknown.Here,we report that PEDV nsp14 is an NF-κB pathway antagonist.Overexpression PEDV nsp14 protein remarkably decreases SeV-,poly(I:C)-and TNF-α-induced NF-κB activation.Meanwhile,expression of proinflammatory cytokines is suppressed by nspl4.nsp14 inhibits the phosphorylation of IKKs by interacting with IKKs and p65.Furthermore,nsp14 suppresses TNF-α-induced phosphorylation and nuclear import of p65.Overexpression nsp14 considerably increases PEDV replication.These results suggest a novel mechanism employed by PEDV to suppress the host antiviral response,providing insights that can guide the development of antivirals against CoVs. | Shasha Li Fan Yang Caina Ma Weijun Cao Jinping Yang Zhenxiang Zhao Hong Tian Zixiang Zhu Haixue Zheng | 2021 | Animal Diseases2021，1，3： | 0 |
| 18 | Boosting ferroptosis and microtubule inhibition for antitumor therapy via a carrier-free supermolecule nanoreactor显示文摘Traditional microtubule inhibitors fail to significantly enhance+e effect of colorectal cancer;hence,new and efficient strategies are necessary.In+is study,a supramolecular nanoreactor(DOC@TA-Fe^(3+))based on tannic acid(TA),iron ion(Fe^(3+)),and docetaxel(DOC)wi+microtubule inhibition,reactive oxygen species(ROS)generation,and gluta+ione peroxidase 4(GPX4)inhibition,is prepared for ferroptosis/apoptosis treatment.After internalization by CT26 cells,+e DOC@TA-Fe^(3+)nanoreactor escapes from+e lysosomes to release payloads.+e subsequent Fe^(3+)/Fe^(2+)conversion mediated by TA reducibility can trigger+e Fenton reaction to enhance+e ROS concentration.Additionally,Fe^(3+)can consume gluta+ione to repress+e activity of GPX4 to induce ferroptosis.Meanwhile,+e released DOC controls microtubule dynamics to activate+e apoptosis pa+way.+e superior in vivo antitumor efficacy of DOC@TA-Fe^(3+)nanoreactor in terms of tumor grow+inhibition and improved survival is verified in CT26 tumor-bearing mouse model.+erefore,+e nanoreactor can act as an effective apoptosis and ferroptosis inducer for application in colorectal cancer+erapy. | Min Mu Xiaoyan Liang Na Zhao Di Chuan Bo Chen Shasha Zhao Guoqing Wang Rangrang Fan Bingwen Zou Bo Han Gang Guo | 2023 | Journal of Pharmaceutical Analysis2023，13，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员